pycom12g00010
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0006438
Physical Location & Seq
Reverse (-)
4192 .. 5609
1418 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g00010.3

Sequence Viewer

Length: 1179 bp
ATGGATGATTTTGACTTAGAATTGGAAGAGATGGAACTAACTGCAGCAGCTGCTGGTTACTATTATTATAACAGTATTACCAGGCAACCTTGTCGTGGTCTGTCACCTAGTAAAGCATCTGGATTTATGACCGAAGTCCTGAGTGGTCATGATGATGTGTTCCAGGAAATGTTTCGGATGGATAAACATGTGTTTCACAAGTTATGTGACATTCTCCGGCAAAGAGGAATGCTACGTGATACTGCTGGTGTTATGATAGAGGAGCAGCTGGGAATCTTTTTGAATATTATTGGCCATAATGAACGCAACAGAGTTATCCAAGAGCGATTTCATCATTCAGGTGAAACCATCAGCCGTCATTTCAATAATGTGTTGAAGGCAGTCAAGTCACTGTCACGCGAATTTCTACAAACACCAACCCCCACCAGACCTTCAGAAATCCGTGGAAGCATTAGATTTTATCCATATTTCCAGGATTGTCTCGGAGTCATTGGTGGAATGCACATTCCAGCCCATGTCCCAGCCAAAGATCAATCCCGGTTTCGAAATAAGAAAGGTGTTCTTTCACAAAATGTGTTGGCAGCTTGCACGTTTGACCTTCAGTTTATATTCATTTATCCTGGCTGGGAAGGCTCAGCTGCAGGAGCCCGTGTGTTGAAGGCAGTCCTTGATGATCCGCATCAGAATTTCCCTTGTATTCCCGAAGGAAAATATTACCTTGTCGACCCAGGATATGCAAATATGAAAGGCTTTATTGCCCCATTTCAAGGCGTCAGGTATCATGCTAATGAATATAGAGGTGCAAATCAATTACCTAGAAATGCAAGGGAGCTATTTAATCACAGGCACTCATCTCTTAGGAATGTCATCCAGAGATCTTTTTATTTGTTGAAGACTAGGTTTCCGATACTCAAACTTGCTCCTCAATACGCATTTCATATTCAAAGGGATATAGTTATAGCTGCGTGTGTTCTACACAATTACATCCGGCGTATGTCAAAAAAGGATTGGCTGTTTTCGAGTGCGGAAGGGACAACAATAGATGAATTGCCTGATCTCGATGAAAATCTTGACACAGAGTTACTTACTTCGATACAAGAACATGTTGCTTTATCAGTGAGAGAATCAATTGCCACAGAAATGTGGGATGACTTTATAAATAAATGGGATCAATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

393

Amino Acids

45.26

Weight (kDa)

6.76

Isoelectric Point (pI)

51.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 69, 1157
AccI GTMKAC 1 cut(s) 723
AccII CGCG 1 cut(s) 399
AciI CCGC 2 cut(s) 677, 1025
AclWI GGATC 2 cut(s) 668, 1176
AcoI YGGCCR 1 cut(s) 292
AcsI RAATTY 2 cut(s) 401, 685
AcuI CTGAAG 2 cut(s) 417, 584
AcyI GRCGYC 1 cut(s) 771
AfiI CCNNNNNNNGG 2 cut(s) 95, 767
AflIII ACRYGT 2 cut(s) 187, 1102
AgsI TTSAA 7 cut(s) 283, 364, 376, 658, 767, 892, 944
AjnI CCWGG 5 cut(s) 80, 162, 471, 619, 727
AjuI GAANNNNNNNTTGG 2 cut(s) 312, 344
AluBI AGCT 6 cut(s) 50, 268, 584, 638, 832, 962
AluI AGCT 6 cut(s) 50, 268, 584, 638, 832, 962
Alw26I GTCTC 1 cut(s) 485
AlwI GGATC 2 cut(s) 668, 1176
AlwNI CAGNNNCTG 2 cut(s) 50, 53
AoxI GGCC 1 cut(s) 292
ApeKI GCWGC 7 cut(s) 44, 47, 50, 265, 581, 638, 962
ApoI RAATTY 2 cut(s) 401, 685
Asp700I GAANNNNTTC 1 cut(s) 171
AsuC2I CCSGG 1 cut(s) 538
AsuHPI GGTGA 2 cut(s) 96, 353
AsuII TTCGAA 1 cut(s) 544
BalI TGGCCA 1 cut(s) 294
BanII GRGCYC 1 cut(s) 649
BbsI GAAGAC 1 cut(s) 899
BbvI GCAGC 7 cut(s) 37, 56, 59, 277, 593, 625, 949
BccI CCATC 3 cut(s) 25, 172, 356
BceAI ACGGC 1 cut(s) 339
BcgI CGANNNNNNTGC 2 cut(s) 74, 108
BciT130I CCWGG 5 cut(s) 82, 164, 473, 621, 729
BcnI CCSGG 1 cut(s) 538
BcoDI GTCTC 1 cut(s) 485
BfaI CTAG 3 cut(s) 108, 816, 897
BfmI CTRYAG 2 cut(s) 42, 639
BglII AGATCT 1 cut(s) 875
BisI GCNGC 7 cut(s) 45, 48, 51, 266, 582, 639, 963
BlpI GCTNAGC 1 cut(s) 634
BlsI GCNGC 7 cut(s) 46, 49, 52, 267, 583, 640, 964
Bme1390I CCNGG 6 cut(s) 82, 164, 473, 538, 621, 729
BmiI GGNNCC 1 cut(s) 646
BmrFI CCNGG 6 cut(s) 82, 164, 473, 538, 621, 729
BmsI GCATC 2 cut(s) 125, 688
BoxI GACNNNNGTC 1 cut(s) 134
BpiI GAAGAC 1 cut(s) 899
Bpu1102I GCTNAGC 1 cut(s) 634
Bpu14I TTCGAA 1 cut(s) 544
BpuMI CCSGG 1 cut(s) 538
BsaAI YACGTR 1 cut(s) 236
BsaBI GATNNNNATC 2 cut(s) 678, 1065
BsaHI GRCGYC 1 cut(s) 771
BsaJI CCNNGG 2 cut(s) 442, 727
BsaXI ACNNNNNCTCC 2 cut(s) 636, 666
Bsc4I CCNNNNNNNGG 2 cut(s) 95, 767
Bse8I GATNNNNATC 2 cut(s) 678, 1065
BseBI CCWGG 5 cut(s) 82, 164, 473, 621, 729
BseDI CCNNGG 2 cut(s) 442, 727
BseGI GGATG 5 cut(s) 10, 183, 867, 984, 1153
BseJI GATNNNNATC 2 cut(s) 678, 1065
BseLI CCNNNNNNNGG 2 cut(s) 95, 767
BseMII CTCAG 2 cut(s) 131, 648
BseRI GAGGAG 2 cut(s) 275, 912
BseXI GCAGC 7 cut(s) 37, 56, 59, 277, 593, 625, 949
BseYI CCCAGC 3 cut(s) 268, 520, 624
Bsh1236I CGCG 1 cut(s) 399
BshFI GGCC 1 cut(s) 294
BsiSI CCGG 3 cut(s) 217, 538, 988
BslFI GGGAC 2 cut(s) 503, 1045
BslI CCNNNNNNNGG 2 cut(s) 95, 767
BsmAI GTCTC 1 cut(s) 485
BsmFI GGGAC 2 cut(s) 503, 1045
BsmI GAATGC 2 cut(s) 234, 504
BsnI GGCC 1 cut(s) 294
Bsp119I TTCGAA 1 cut(s) 544
Bsp1286I GDGCHC 1 cut(s) 649
Bsp143I GATC 5 cut(s) 529, 673, 875, 1054, 1168
Bsp1720I GCTNAGC 1 cut(s) 634
BspACI CCGC 2 cut(s) 677, 1025
BspANI GGCC 1 cut(s) 294
BspCNI CTCAG 2 cut(s) 132, 647
BspFNI CGCG 1 cut(s) 399
BspHI TCATGA 1 cut(s) 148
BspLI GGNNCC 1 cut(s) 646
BspMAI CTGCAG 2 cut(s) 46, 643
BspPI GGATC 2 cut(s) 668, 1176
BspT104I TTCGAA 1 cut(s) 544
BssECI CCNNGG 2 cut(s) 442, 727
BssMI GATC 5 cut(s) 529, 673, 875, 1054, 1168
BssNI GRCGYC 1 cut(s) 771
Bst2UI CCWGG 5 cut(s) 82, 164, 473, 621, 729
Bst4CI ACNGT 2 cut(s) 74, 393
Bst6I CTCTTC 1 cut(s) 21
BstACI GRCGYC 1 cut(s) 771
BstAPI GCANNNNNTGC 1 cut(s) 50
BstBAI YACGTR 1 cut(s) 236
BstBI TTCGAA 1 cut(s) 544
BstC8I GCNNGC 1 cut(s) 586
BstDEI CTNAG 4 cut(s) 16, 140, 634, 857
BstDSI CCRYGG 1 cut(s) 442
BstF5I GGATG 5 cut(s) 10, 183, 867, 984, 1153
BstFNI CGCG 1 cut(s) 399
BstKTI GATC 5 cut(s) 532, 676, 878, 1057, 1171
BstMAI GTCTC 1 cut(s) 485
BstMBI GATC 5 cut(s) 529, 673, 875, 1054, 1168
BstMWI GCNNNNNNNGC 3 cut(s) 50, 630, 644
BstNI CCWGG 5 cut(s) 82, 164, 473, 621, 729
BstNSI RCATGY 2 cut(s) 191, 1106
BstPAI GACNNNNGTC 1 cut(s) 134
BstSCI CCNGG 6 cut(s) 80, 162, 471, 536, 619, 727
BstSFI CTRYAG 2 cut(s) 42, 639
BstUI CGCG 1 cut(s) 399
BstV1I GCAGC 7 cut(s) 37, 56, 59, 277, 593, 625, 949
BstV2I GAAGAC 1 cut(s) 899
BstX2I RGATCY 1 cut(s) 875
BstYI RGATCY 1 cut(s) 875
BsuRI GGCC 1 cut(s) 294
BtgI CCRYGG 1 cut(s) 442
BtsCI GGATG 5 cut(s) 10, 183, 867, 984, 1153
BtsIMutI CAGTG 2 cut(s) 389, 1122
Cac8I GCNNGC 1 cut(s) 586
CaiI CAGNNNCTG 2 cut(s) 50, 53
CciI TCATGA 1 cut(s) 148
CseI GACGC 1 cut(s) 760
CviAII CATG 5 cut(s) 149, 188, 515, 782, 1103
DdeI CTNAG 4 cut(s) 16, 140, 634, 857
DpnI GATC 5 cut(s) 531, 675, 877, 1056, 1170
DpnII GATC 5 cut(s) 529, 673, 875, 1054, 1168
EaeI YGGCCR 1 cut(s) 292
Eam1104I CTCTTC 1 cut(s) 21
EarI CTCTTC 1 cut(s) 21
Eco24I GRGCYC 1 cut(s) 649
Eco57I CTGAAG 2 cut(s) 417, 584
EcoRII CCWGG 5 cut(s) 80, 162, 471, 619, 727
EcoT38I GRGCYC 1 cut(s) 649
FaeI CATG 5 cut(s) 152, 191, 518, 785, 1106
FalI AAGNNNNNCTT 2 cut(s) 546, 578
FaqI GGGAC 2 cut(s) 503, 1045
FatI CATG 5 cut(s) 148, 187, 514, 781, 1102
FblI GTMKAC 1 cut(s) 723
Fnu4HI GCNGC 7 cut(s) 45, 48, 51, 266, 582, 639, 963
FokI GGATG 5 cut(s) 17, 190, 854, 971, 1160
FriOI GRGCYC 1 cut(s) 649
Fsp4HI GCNGC 7 cut(s) 45, 48, 51, 266, 582, 639, 963
FspBI CTAG 3 cut(s) 108, 816, 897
GluI GCNGC 7 cut(s) 45, 48, 51, 266, 582, 639, 963
GsaI CCCAGC 3 cut(s) 272, 524, 628
HaeIII GGCC 1 cut(s) 294
HapII CCGG 3 cut(s) 217, 538, 988
HgaI GACGC 1 cut(s) 760
Hin1I GRCGYC 1 cut(s) 771
Hin1II CATG 5 cut(s) 152, 191, 518, 785, 1106
HincII GTYRAC 1 cut(s) 724
HindII GTYRAC 1 cut(s) 724
HinfI GANTC 3 cut(s) 273, 486, 1124
HpaII CCGG 3 cut(s) 217, 538, 988
HphI GGTGA 2 cut(s) 96, 353
Hpy166II GTNNAC 1 cut(s) 724
Hpy188I TCNGA 5 cut(s) 177, 436, 485, 684, 906
Hpy188III TCNNGA 7 cut(s) 120, 139, 149, 701, 871, 1058, 1070
Hpy8I GTNNAC 1 cut(s) 724
HpyAV CCTTC 7 cut(s) 370, 441, 608, 623, 652, 698, 1022
HpyCH4III ACNGT 2 cut(s) 74, 393
HpyCH4IV ACGT 2 cut(s) 235, 590
HpyCH4V TGCA 7 cut(s) 44, 502, 588, 641, 737, 803, 824
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 630, 644
HpyF3I CTNAG 4 cut(s) 16, 140, 634, 857
HpySE526I ACGT 2 cut(s) 235, 590
Hsp92I GRCGYC 1 cut(s) 771
Hsp92II CATG 5 cut(s) 152, 191, 518, 785, 1106
Kzo9I GATC 5 cut(s) 529, 673, 875, 1054, 1168
LmnI GCTCC 4 cut(s) 262, 644, 829, 925
Lsp1109I GCAGC 7 cut(s) 37, 56, 59, 277, 593, 625, 949
LweI GCATC 2 cut(s) 125, 688
MaeI CTAG 3 cut(s) 108, 816, 897
MaeII ACGT 2 cut(s) 235, 590
MaeIII GTNAC 6 cut(s) 56, 102, 206, 387, 393, 1080
MalI GATC 5 cut(s) 531, 675, 877, 1056, 1170
MboI GATC 5 cut(s) 529, 673, 875, 1054, 1168
MboII GAAGA 2 cut(s) 38, 904
MfeI CAATTG 1 cut(s) 1128
MflI RGATCY 1 cut(s) 875
MhlI GDGCHC 1 cut(s) 649
MlsI TGGCCA 1 cut(s) 294
MluCI AATT 7 cut(s) 20, 401, 685, 809, 979, 1046, 1128
MluNI TGGCCA 1 cut(s) 294
MlyI GAGTC 1 cut(s) 495
MnlI CCTC 4 cut(s) 218, 253, 791, 933
Mox20I TGGCCA 1 cut(s) 294
MroXI GAANNNNTTC 1 cut(s) 171
MscI TGGCCA 1 cut(s) 294
MseI TTAA 1 cut(s) 837
MslI CAYNNNNRTG 4 cut(s) 153, 339, 786, 1139
Msp20I TGGCCA 1 cut(s) 294
MspA1I CMGCKG 3 cut(s) 50, 268, 638
MspI CCGG 3 cut(s) 217, 538, 988
MspR9I CCNGG 6 cut(s) 82, 164, 473, 538, 621, 729
MunI CAATTG 1 cut(s) 1128
Mva1269I GAATGC 2 cut(s) 234, 504
MvaI CCWGG 5 cut(s) 82, 164, 473, 621, 729
MvnI CGCG 1 cut(s) 399
MwoI GCNNNNNNNGC 3 cut(s) 50, 630, 644
NciI CCSGG 1 cut(s) 538
NdeII GATC 5 cut(s) 529, 673, 875, 1054, 1168
NlaIII CATG 5 cut(s) 152, 191, 518, 785, 1106
NlaIV GGNNCC 1 cut(s) 646
NmuCI GTSAC 4 cut(s) 102, 206, 387, 393
NspI RCATGY 2 cut(s) 191, 1106
NspV TTCGAA 1 cut(s) 544
PagI TCATGA 1 cut(s) 148
PciI ACATGT 2 cut(s) 187, 1102
PctI GAATGC 2 cut(s) 234, 504
PdmI GAANNNNTTC 1 cut(s) 171
PfeI GAWTC 2 cut(s) 273, 1124
PfoI TCCNGGA 2 cut(s) 162, 471
PkrI GCNGC 7 cut(s) 46, 49, 52, 267, 583, 640, 964
PleI GAGTC 1 cut(s) 494
PpsI GAGTC 1 cut(s) 494
Ppu21I YACGTR 1 cut(s) 236
PscI ACATGT 2 cut(s) 187, 1102
PshAI GACNNNNGTC 1 cut(s) 134
PsiI TTATAA 2 cut(s) 69, 1157
Psp6I CCWGG 5 cut(s) 80, 162, 471, 619, 727
PspFI CCCAGC 3 cut(s) 268, 520, 624
PspGI CCWGG 5 cut(s) 80, 162, 471, 619, 727
PspN4I GGNNCC 1 cut(s) 646
PstI CTGCAG 2 cut(s) 46, 643
PstNI CAGNNNCTG 2 cut(s) 50, 53
PsuI RGATCY 1 cut(s) 875
PvuII CAGCTG 3 cut(s) 50, 268, 638
RseI CAYNNNNRTG 4 cut(s) 153, 339, 786, 1139
SalI GTCGAC 1 cut(s) 722
SaqAI TTAA 1 cut(s) 837
SatI GCNGC 7 cut(s) 45, 48, 51, 266, 582, 639, 963
Sau3AI GATC 5 cut(s) 529, 673, 875, 1054, 1168
SchI GAGTC 1 cut(s) 495
ScrFI CCNGG 6 cut(s) 82, 164, 473, 538, 621, 729
SduI GDGCHC 1 cut(s) 649
SfaNI GCATC 2 cut(s) 125, 688
SfcI CTRYAG 2 cut(s) 42, 639
SfuI TTCGAA 1 cut(s) 544
SmiMI CAYNNNNRTG 4 cut(s) 153, 339, 786, 1139
Sse9I AATT 7 cut(s) 20, 401, 685, 809, 979, 1046, 1128
SsiI CCGC 2 cut(s) 677, 1025
SspI AATATT 2 cut(s) 286, 713
SspMI CTAG 3 cut(s) 108, 816, 897
StyD4I CCNGG 6 cut(s) 80, 162, 471, 536, 619, 727
TaaI ACNGT 2 cut(s) 74, 393
TaiI ACGT 2 cut(s) 238, 593
TaqI TCGA 5 cut(s) 544, 723, 1019, 1059, 1091
TaqII GACCGA 1 cut(s) 146
TasI AATT 7 cut(s) 20, 401, 685, 809, 979, 1046, 1128
TfiI GAWTC 2 cut(s) 273, 1124
Tru1I TTAA 1 cut(s) 837
Tru9I TTAA 1 cut(s) 837
TscAI CASTG 2 cut(s) 396, 1122
TseFI GTSAC 4 cut(s) 102, 206, 387, 393
TseI GCWGC 7 cut(s) 44, 47, 50, 265, 581, 638, 962
Tsp45I GTSAC 4 cut(s) 102, 206, 387, 393
TspDTI ATGAA 8 cut(s) 315, 320, 601, 758, 804, 926, 1059, 1077
TspGWI ACGGA 1 cut(s) 431
TspRI CASTG 2 cut(s) 396, 1122
XapI RAATTY 2 cut(s) 401, 685
XceI RCATGY 2 cut(s) 191, 1106
XmiI GTMKAC 1 cut(s) 723
XmnI GAANNNNTTC 1 cut(s) 171
XspI CTAG 3 cut(s) 108, 816, 897
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.