pycom01g04860

IST1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0015717
Physical Location & Seq
Reverse (-)
4150247 .. 4151509
1263 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g04860.1

Sequence Viewer

Length: 1161 bp
ATGTGTTCCGAGGAAGTTGAATCGATCGAGGTCATTCACCACAAATCTCCAAACGGCGACCACAACCCACTGCGAATTCCATCGACAATCCACGACCATTCCATTCCGCCATTGCTTTCATCTTCACCGATGTTGCGAGCACTGGCAAATTCAACCGTCAAGCCACACTCGAACAACTCCGCTGCATCTACAGTTTTGCTTCCGGAATCGAAGACAATGACCTTTGGCCCGCAACTACTCCATAAGCCCTTGTTCTCCGATACTACGAAAATTGGACCTAAGGTTTATTGGTATCTTGTGGGAAAATTTGTGAAGGAAGATTTTTATGCATTGGAAGTGTTTGAGAAAATGTCTGAGAAAGGGAGTGAAGACCAAATGGAAATATCTCACTGGAAAGATATCTACAATTTCGAGAACAGTAGTTGTATCTGGAATTCCTGTGGCTGCACATTTGTTGTTGAGCTTTTAGTAAGTCAAAATCAAGGTATCTCTGCTACTTTTCACCAATATGCTACAAGTGGACCACAAGTTGAGGATGACCTTTCCCAATTTCAAAATGTGGAGTTCGATCATGTTGCGAAGGCAATTGAGAATGTGGGGGTTAGATGGATAGCACTACCAACTGCTTCAATCTTATTTCATGATTTTGATGAAAAGTCTAAGAGACAAGGTGAATGCACTATGGGCACCTATTTTGAATTCATATTCTCTCTCAAGGATCACCAGGAAATCAATATTGGTTCCTTAACTGTCGATCTACATGCTAATTTCCCTGTGTCGTTCACTAGTAACCTCAACTTTCGGCCAGTATGGGATCTCGGAGATGCATTGGCTCAAGCTGTAAAGCTGATGCTCTATCTTCAGTGCAAGGAGGGCAAGCACATGGATAATCTTTTCAATGTTCAAAGACTTTTGCACTTGTTAGTAGTGTATAGCAACATAAGAGGGGCATTGCTTTATGAGATTGAGCGGTACAAAAAGAAAGCCTTAGTTAAGCAACTTGGTTGTCTTGCCATCCAGCTCCACTGTTCTACTTTCAATTTGGAAACAAATAATGAAGAAAATTGGTCCAAGGAGCATAATGGAGCTAATATTACTGTAGTTATGTTCCTTATTGTTGGTACAAATGTTCCCATTGAACAAGAGAGCTTTATTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

387

Amino Acids

43.73

Weight (kDa)

5.62

Isoelectric Point (pI)

46.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 686
AccBSI CCGCTC 1 cut(s) 970
AccIII TCCGGA 1 cut(s) 202
AciI CCGC 4 cut(s) 107, 180, 230, 970
AclWI GGATC 2 cut(s) 726, 822
AcoI YGGCCR 1 cut(s) 803
AcsI RAATTY 5 cut(s) 75, 148, 305, 433, 698
AcuI CTGAAG 1 cut(s) 845
AfaI GTAC 2 cut(s) 974, 1123
AgsI TTSAA 9 cut(s) 20, 153, 554, 630, 698, 898, 905, 1039, 1139
AhlI ACTAGT 1 cut(s) 785
AjnI CCWGG 1 cut(s) 723
AjuI GAANNNNNNNTTGG 2 cut(s) 720, 752
AluBI AGCT 6 cut(s) 463, 839, 847, 1021, 1088, 1149
AluI AGCT 6 cut(s) 463, 839, 847, 1021, 1088, 1149
Alw21I GWGCWC 1 cut(s) 142
Alw26I GTCTC 1 cut(s) 658
AlwI GGATC 2 cut(s) 726, 822
Aor13HI TCCGGA 1 cut(s) 202
AoxI GGCC 2 cut(s) 226, 803
ApeKI GCWGC 2 cut(s) 182, 444
ApoI RAATTY 5 cut(s) 75, 148, 305, 433, 698
AspS9I GGNCC 4 cut(s) 227, 275, 521, 1068
AsuHPI GGTGA 5 cut(s) 29, 117, 494, 683, 713
AvaII GGWCC 3 cut(s) 275, 521, 1068
AxyI CCTNAGG 1 cut(s) 279
BaeGI GKGCMC 1 cut(s) 689
BaeI ACNNNNGTAYC 2 cut(s) 1113, 1146
BanI GGYRCC 1 cut(s) 686
BbsI GAAGAC 2 cut(s) 218, 375
Bbv12I GWGCWC 1 cut(s) 142
BbvI GCAGC 2 cut(s) 169, 431
BccI CCATC 3 cut(s) 88, 600, 1022
BceAI ACGGC 1 cut(s) 70
BcgI CGANNNNNNTGC 4 cut(s) 557, 591, 743, 777
BciT130I CCWGG 1 cut(s) 725
BcoDI GTCTC 1 cut(s) 658
BcuI ACTAGT 1 cut(s) 785
BfaI CTAG 1 cut(s) 786
BfmI CTRYAG 2 cut(s) 189, 1098
BisI GCNGC 2 cut(s) 183, 445
BlsI GCNGC 2 cut(s) 184, 446
Bme1390I CCNGG 1 cut(s) 725
Bme18I GGWCC 3 cut(s) 275, 521, 1068
BmgT120I GGNCC 4 cut(s) 227, 275, 521, 1068
BmiI GGNNCC 2 cut(s) 688, 742
BmrFI CCNGG 1 cut(s) 725
BmsI GCATC 3 cut(s) 194, 814, 840
BpiI GAAGAC 2 cut(s) 218, 375
BpuEI CTTGAG 2 cut(s) 698, 819
Bsa29I ATCGAT 1 cut(s) 23
BsaJI CCNNGG 2 cut(s) 9, 1071
BsaWI WCCGGW 1 cut(s) 202
Bse1I ACTGG 3 cut(s) 147, 395, 806
Bse21I CCTNAGG 1 cut(s) 279
Bse3DI GCAATG 2 cut(s) 110, 950
BseAI TCCGGA 1 cut(s) 202
BseBI CCWGG 1 cut(s) 725
BseCI ATCGAT 1 cut(s) 23
BseDI CCNNGG 2 cut(s) 9, 1071
BseGI GGATG 2 cut(s) 541, 1014
BseMI GCAATG 2 cut(s) 110, 950
BseMII CTCAG 1 cut(s) 345
BseNI ACTGG 3 cut(s) 147, 395, 806
BseSI GKGCMC 1 cut(s) 689
BseXI GCAGC 2 cut(s) 169, 431
BsgI GTGCAG 1 cut(s) 430
Bsh1285I CGRYCG 1 cut(s) 27
BshFI GGCC 2 cut(s) 228, 805
BshNI GGYRCC 1 cut(s) 686
BshVI ATCGAT 1 cut(s) 23
BsiEI CGRYCG 1 cut(s) 27
BsiHKAI GWGCWC 1 cut(s) 142
BsiSI CCGG 1 cut(s) 203
BsmAI GTCTC 1 cut(s) 658
BsmI GAATGC 1 cut(s) 680
BsnI GGCC 2 cut(s) 228, 805
Bsp1286I GDGCHC 2 cut(s) 142, 689
Bsp13I TCCGGA 1 cut(s) 202
Bsp143I GATC 5 cut(s) 24, 568, 718, 754, 814
BspACI CCGC 4 cut(s) 107, 180, 230, 970
BspANI GGCC 2 cut(s) 228, 805
BspCNI CTCAG 1 cut(s) 346
BspDI ATCGAT 1 cut(s) 23
BspEI TCCGGA 1 cut(s) 202
BspHI TCATGA 1 cut(s) 640
BspLI GGNNCC 2 cut(s) 688, 742
BspPI GGATC 2 cut(s) 726, 822
BspT107I GGYRCC 1 cut(s) 686
BsrBI CCGCTC 1 cut(s) 970
BsrDI GCAATG 2 cut(s) 110, 950
BsrI ACTGG 3 cut(s) 147, 395, 806
BssECI CCNNGG 2 cut(s) 9, 1071
BssMI GATC 5 cut(s) 24, 568, 718, 754, 814
BssT1I CCWWGG 1 cut(s) 1071
Bst2UI CCWGG 1 cut(s) 725
Bst4CI ACNGT 6 cut(s) 157, 193, 419, 751, 1028, 1099
BstC8I GCNNGC 3 cut(s) 138, 230, 878
BstDEI CTNAG 4 cut(s) 279, 354, 660, 988
BstF5I GGATG 2 cut(s) 541, 1014
BstKTI GATC 5 cut(s) 27, 571, 721, 757, 817
BstMAI GTCTC 1 cut(s) 658
BstMBI GATC 5 cut(s) 24, 568, 718, 754, 814
BstMCI CGRYCG 1 cut(s) 27
BstMWI GCNNNNNNNGC 2 cut(s) 684, 873
BstNI CCWGG 1 cut(s) 725
BstNSI RCATGY 1 cut(s) 764
BstSCI CCNGG 1 cut(s) 723
BstSFI CTRYAG 2 cut(s) 189, 1098
BstSLI GKGCMC 1 cut(s) 689
BstV1I GCAGC 2 cut(s) 169, 431
BstV2I GAAGAC 2 cut(s) 218, 375
BstX2I RGATCY 1 cut(s) 814
BstYI RGATCY 1 cut(s) 814
Bsu15I ATCGAT 1 cut(s) 23
Bsu36I CCTNAGG 1 cut(s) 279
BsuRI GGCC 2 cut(s) 228, 805
BsuTUI ATCGAT 1 cut(s) 23
BtsCI GGATG 2 cut(s) 541, 1014
BtsI GCAGTG 1 cut(s) 68
BtsIMutI CAGTG 5 cut(s) 68, 140, 388, 869, 1024
Cac8I GCNNGC 3 cut(s) 138, 230, 878
CciI TCATGA 1 cut(s) 640
Cfr13I GGNCC 4 cut(s) 227, 275, 521, 1068
ClaI ATCGAT 1 cut(s) 23
Csp6I GTAC 2 cut(s) 973, 1122
CviAII CATG 4 cut(s) 572, 641, 761, 883
CviQI GTAC 2 cut(s) 973, 1122
DdeI CTNAG 4 cut(s) 279, 354, 660, 988
DpnI GATC 5 cut(s) 26, 570, 720, 756, 816
DpnII GATC 5 cut(s) 24, 568, 718, 754, 814
EaeI YGGCCR 1 cut(s) 803
EciI GGCGGA 1 cut(s) 96
Eco130I CCWWGG 1 cut(s) 1071
Eco32I GATATC 1 cut(s) 400
Eco47I GGWCC 3 cut(s) 275, 521, 1068
Eco57I CTGAAG 1 cut(s) 845
Eco81I CCTNAGG 1 cut(s) 279
EcoRI GAATTC 3 cut(s) 75, 433, 698
EcoRII CCWGG 1 cut(s) 723
EcoRV GATATC 1 cut(s) 400
EcoT14I CCWWGG 1 cut(s) 1071
EcoT22I ATGCAT 2 cut(s) 331, 829
ErhI CCWWGG 1 cut(s) 1071
FaeI CATG 4 cut(s) 575, 644, 764, 886
FalI AAGNNNNNCTT 2 cut(s) 971, 1003
FatI CATG 4 cut(s) 571, 640, 760, 882
FauI CCCGC 1 cut(s) 237
Fnu4HI GCNGC 2 cut(s) 183, 445
FokI GGATG 2 cut(s) 548, 1001
Fsp4HI GCNGC 2 cut(s) 183, 445
FspBI CTAG 1 cut(s) 786
GluI GCNGC 2 cut(s) 183, 445
HaeIII GGCC 2 cut(s) 228, 805
HapII CCGG 1 cut(s) 203
Hin1II CATG 4 cut(s) 575, 644, 764, 886
HinfI GANTC 2 cut(s) 20, 206
HpaII CCGG 1 cut(s) 203
HphI GGTGA 5 cut(s) 29, 117, 494, 683, 713
Hpy166II GTNNAC 2 cut(s) 521, 783
Hpy188I TCNGA 4 cut(s) 10, 259, 355, 821
Hpy188III TCNNGA 4 cut(s) 203, 412, 430, 641
Hpy8I GTNNAC 2 cut(s) 521, 783
HpyAV CCTTC 2 cut(s) 307, 574
HpyCH4III ACNGT 6 cut(s) 157, 193, 419, 751, 1028, 1099
HpyCH4V TGCA 7 cut(s) 185, 329, 447, 678, 827, 867, 916
HpyF10VI GCNNNNNNNGC 2 cut(s) 684, 873
HpyF3I CTNAG 4 cut(s) 279, 354, 660, 988
Hsp92II CATG 4 cut(s) 575, 644, 764, 886
Kpn2I TCCGGA 1 cut(s) 202
Kzo9I GATC 5 cut(s) 24, 568, 718, 754, 814
LmnI GCTCC 3 cut(s) 1026, 1075, 1085
Lsp1109I GCAGC 2 cut(s) 169, 431
LweI GCATC 3 cut(s) 194, 814, 840
MaeI CTAG 1 cut(s) 786
MaeIII GTNAC 1 cut(s) 788
MalI GATC 5 cut(s) 26, 570, 720, 756, 816
MbiI CCGCTC 1 cut(s) 970
MboI GATC 5 cut(s) 24, 568, 718, 754, 814
MboII GAAGA 6 cut(s) 114, 223, 329, 380, 851, 1070
MfeI CAATTG 1 cut(s) 585
MflI RGATCY 1 cut(s) 814
MhlI GDGCHC 2 cut(s) 142, 689
MnlI CCTC 6 cut(s) 4, 22, 526, 803, 865, 938
Mph1103I ATGCAT 2 cut(s) 331, 829
MroI TCCGGA 1 cut(s) 202
MseI TTAA 2 cut(s) 746, 993
MslI CAYNNNNRTG 1 cut(s) 507
MspA1I CMGCKG 1 cut(s) 182
MspI CCGG 1 cut(s) 203
MspR9I CCNGG 1 cut(s) 725
MunI CAATTG 1 cut(s) 585
Mva1269I GAATGC 1 cut(s) 680
MvaI CCWGG 1 cut(s) 725
MwoI GCNNNNNNNGC 2 cut(s) 684, 873
NdeII GATC 5 cut(s) 24, 568, 718, 754, 814
NlaIII CATG 4 cut(s) 575, 644, 764, 886
NlaIV GGNNCC 2 cut(s) 688, 742
NsiI ATGCAT 2 cut(s) 331, 829
NspI RCATGY 1 cut(s) 764
PagI TCATGA 1 cut(s) 640
PctI GAATGC 1 cut(s) 680
PfeI GAWTC 2 cut(s) 20, 206
PkrI GCNGC 2 cut(s) 184, 446
Ple19I CGATCG 1 cut(s) 27
Psp6I CCWGG 1 cut(s) 723
PspGI CCWGG 1 cut(s) 723
PspN4I GGNNCC 2 cut(s) 688, 742
PspPI GGNCC 4 cut(s) 227, 275, 521, 1068
PsuI RGATCY 1 cut(s) 814
PvuI CGATCG 1 cut(s) 27
RsaI GTAC 2 cut(s) 974, 1123
RsaNI GTAC 2 cut(s) 973, 1122
RseI CAYNNNNRTG 1 cut(s) 507
SaqAI TTAA 2 cut(s) 746, 993
SatI GCNGC 2 cut(s) 183, 445
Sau3AI GATC 5 cut(s) 24, 568, 718, 754, 814
Sau96I GGNCC 4 cut(s) 227, 275, 521, 1068
ScrFI CCNGG 1 cut(s) 725
SduI GDGCHC 2 cut(s) 142, 689
SfaNI GCATC 3 cut(s) 194, 814, 840
SfcI CTRYAG 2 cut(s) 189, 1098
SinI GGWCC 3 cut(s) 275, 521, 1068
SmiMI CAYNNNNRTG 1 cut(s) 507
SmlI CTYRAG 2 cut(s) 713, 834
SmoI CTYRAG 2 cut(s) 713, 834
SpeI ACTAGT 1 cut(s) 785
SsiI CCGC 4 cut(s) 107, 180, 230, 970
SspI AATATT 2 cut(s) 736, 1093
SspMI CTAG 1 cut(s) 786
StyD4I CCNGG 1 cut(s) 723
StyI CCWWGG 1 cut(s) 1071
TaaI ACNGT 6 cut(s) 157, 193, 419, 751, 1028, 1099
TaqI TCGA 8 cut(s) 23, 27, 83, 170, 209, 411, 567, 753
TfiI GAWTC 2 cut(s) 20, 206
Tru1I TTAA 2 cut(s) 746, 993
Tru9I TTAA 2 cut(s) 746, 993
TscAI CASTG 5 cut(s) 75, 147, 395, 869, 1031
TseI GCWGC 2 cut(s) 182, 444
TspDTI ATGAA 5 cut(s) 108, 629, 666, 691, 1071
TspRI CASTG 5 cut(s) 75, 147, 395, 869, 1031
VpaK11BI GGWCC 3 cut(s) 275, 521, 1068
XapI RAATTY 5 cut(s) 75, 148, 305, 433, 698
XceI RCATGY 1 cut(s) 764
XspI CTAG 1 cut(s) 786
Zsp2I ATGCAT 2 cut(s) 331, 829
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.