pycom13g01890

metallopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0015717
Physical Location & Seq
Forward (+)
1269594 .. 1271660
2067 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g01890.3

Sequence Viewer

Length: 1332 bp
ATGTTGCGAGCACTGGCAAAGTCGGCCGTCAAGCCACACTCGAACAACTCTGCTGCATCTACAGTTATCATTTTAGCAACTCCGGAGGCTAAATTCCCTTCTTTTTTGACTGATTACTATGGTGCATACGTGAAAGATAAATTTGGATGGGTTAATTGGTATCTTGTGGGTAAATTTGTGAAGAAAAATTTCTATGCATTGCATCTGTTTGATGATATGTCTAAGAGGCATAATAGGGAGAATATGTCATTGAGTAACAACCTGGAAGCTTTTGATATTGTTGTACTTGTTTGTCGTTATTTACCTAAAGATCTTGCTGCTAGTTTCACTGAGGAGAATGGGACTTTGGGTTATATCAATGTTATCAAGCAGTTCCTTTGCTTATCACTGTTACACATCTGTGCGTTCACAGTCATGGCCATTTCCCAACTTGACTATTCTATTTTCACCAGCTTGTTATCGAAATTGAATCAGGAATTGTGTAATAGGGTTGATGTGCACCTCGATGCTATTTTATTTCCTAATTGCATAGCGAATGACAAGATTTTGCAGCAACCGGGTTGGTGTCACTCTCTAACTGATCCTTGTGACAGTATATCTTATCAAAGGGACTCAGCTATGGTTGCTGCTAGTGCTCTCAATGATACAATCAGTAAAGGGAAGAACCTATATGTATCGATATTTGTTCAGGAAAGTACGAATGATAACAGGCTCCGTATCTTTTCCAACACTCTGAACCCTGCATTGTCTCAGGATGTGGTCTGTTACAAAGGTCTGGAACTTGCTACAAATATTACAGAAGCATGTAACAATCATGTTTTTGGTCAAATTGTGCTCTTGCTGGTGCAAATTGTTACAATCATAATCAGGCTCTTGGAAGAATATGCTAACTTGAAAGGGCTTCAAGATGGACATTTCAGACAAGAAGAGGAAATGCTTGCAATACTCAGCCATAAACACAGAAATGACCCTCTCAAAATCAAGGAAATCATTTCCATGGTTGCCAACAATCTAACAAGTCTCCTGAATTTAGAGATTGAGTTCAAGCTTCTTTTCTGTTTAAATTTTTGGAACTTGATACTACTCAGCCACCTTATGTGTTCAAATGCCAACTGCCAACTATACACCTTAATAGAGGTGATGAAGGACATGGTTGAAGCTTTGGGTCTGCTCAGGTCCTCTAAATCTTCCATGCATAATATCGGTGTTGCTTATGACGGTTTAAAGTTTAATGATTTCAGCGATTCAGAAAGAATTGTAGTTTTCAATATCCAATATAGGCTAGTCCCATCCATCAGCTTATTTCAGAAAATGGAGTTTTATGACAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
M16C_assoc Peptidase_M16_C

Protein Analysis

444

Amino Acids

50.32

Weight (kDa)

6.19

Isoelectric Point (pI)

42.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 82
AclWI GGATC 1 cut(s) 575
AcoI YGGCCR 2 cut(s) 24, 417
AcsI RAATTY 6 cut(s) 92, 140, 173, 187, 1027, 1063
AfaI GTAC 2 cut(s) 285, 697
AgsI TTSAA 7 cut(s) 469, 895, 905, 1045, 1104, 1157, 1267
AjnI CCWGG 1 cut(s) 261
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AleI CACNNNNGTG 1 cut(s) 399
AluBI AGCT 6 cut(s) 269, 453, 617, 1048, 1160, 1299
AluI AGCT 6 cut(s) 269, 453, 617, 1048, 1160, 1299
Alw21I GWGCWC 4 cut(s) 13, 501, 637, 837
Alw26I GTCTC 2 cut(s) 753, 1025
Alw44I GTGCAC 1 cut(s) 497
AlwI GGATC 1 cut(s) 575
Aor13HI TCCGGA 1 cut(s) 82
AoxI GGCC 2 cut(s) 24, 417
ApaLI GTGCAC 1 cut(s) 497
ApeKI GCWGC 4 cut(s) 53, 317, 550, 626
ApoI RAATTY 6 cut(s) 92, 140, 173, 187, 1027, 1063
Asp700I GAANNNNTTC 1 cut(s) 188
AspS9I GGNCC 1 cut(s) 1176
AsuC2I CCSGG 1 cut(s) 558
AsuHPI GGTGA 2 cut(s) 439, 1150
AvaII GGWCC 1 cut(s) 1176
BaeGI GKGCMC 1 cut(s) 501
BalI TGGCCA 1 cut(s) 419
Bbv12I GWGCWC 4 cut(s) 13, 501, 637, 837
BbvI GCAGC 4 cut(s) 40, 304, 562, 613
BccI CCATC 4 cut(s) 141, 902, 1297, 1301
BceAI ACGGC 1 cut(s) 11
BciT130I CCWGG 1 cut(s) 263
BcnI CCSGG 1 cut(s) 558
BcoDI GTCTC 2 cut(s) 753, 1025
BfaI CTAG 3 cut(s) 321, 630, 1283
BfmI CTRYAG 1 cut(s) 60
BglII AGATCT 1 cut(s) 310
BisI GCNGC 4 cut(s) 54, 318, 551, 627
BlsI GCNGC 4 cut(s) 55, 319, 552, 628
Bme1390I CCNGG 2 cut(s) 263, 558
Bme18I GGWCC 1 cut(s) 1176
BmgT120I GGNCC 1 cut(s) 1176
BmiI GGNNCC 1 cut(s) 713
BmrFI CCNGG 2 cut(s) 263, 558
BmsI GCATC 3 cut(s) 65, 211, 496
Bpu10I CCTNAGC 1 cut(s) 1172
BpuMI CCSGG 1 cut(s) 558
Bsa29I ATCGAT 1 cut(s) 677
BsaAI YACGTR 1 cut(s) 130
BsaJI CCNNGG 1 cut(s) 996
BsaWI WCCGGW 1 cut(s) 82
BsaXI ACNNNNNCTCC 2 cut(s) 230, 260
Bse1I ACTGG 1 cut(s) 18
Bse3DI GCAATG 1 cut(s) 197
BseAI TCCGGA 1 cut(s) 82
BseBI CCWGG 1 cut(s) 263
BseCI ATCGAT 1 cut(s) 677
BseDI CCNNGG 1 cut(s) 996
BseGI GGATG 3 cut(s) 152, 760, 1289
BseMI GCAATG 1 cut(s) 197
BseMII CTCAG 6 cut(s) 321, 627, 764, 961, 1099, 1186
BseNI ACTGG 1 cut(s) 18
BseRI GAGGAG 1 cut(s) 347
BseSI GKGCMC 1 cut(s) 501
BseX3I CGGCCG 1 cut(s) 24
BseXI GCAGC 4 cut(s) 40, 304, 562, 613
Bsh1285I CGRYCG 1 cut(s) 27
BshFI GGCC 2 cut(s) 26, 419
BshVI ATCGAT 1 cut(s) 677
BsiEI CGRYCG 1 cut(s) 27
BsiHKAI GWGCWC 4 cut(s) 13, 501, 637, 837
BsiSI CCGG 2 cut(s) 83, 557
BslFI GGGAC 3 cut(s) 355, 623, 1271
BsmAI GTCTC 2 cut(s) 753, 1025
BsmFI GGGAC 3 cut(s) 355, 623, 1271
BsnI GGCC 2 cut(s) 26, 419
Bsp1286I GDGCHC 4 cut(s) 13, 501, 637, 837
Bsp13I TCCGGA 1 cut(s) 82
Bsp143I GATC 2 cut(s) 310, 580
Bsp19I CCATGG 1 cut(s) 996
BspANI GGCC 2 cut(s) 26, 419
BspCNI CTCAG 6 cut(s) 322, 626, 763, 960, 1098, 1185
BspDI ATCGAT 1 cut(s) 677
BspEI TCCGGA 1 cut(s) 82
BspLI GGNNCC 1 cut(s) 713
BspPI GGATC 1 cut(s) 575
BsrDI GCAATG 1 cut(s) 197
BsrI ACTGG 1 cut(s) 18
BssECI CCNNGG 1 cut(s) 996
BssMI GATC 2 cut(s) 310, 580
BssT1I CCWWGG 1 cut(s) 996
Bst2UI CCWGG 1 cut(s) 263
Bst4CI ACNGT 5 cut(s) 64, 390, 412, 593, 1220
Bst6I CTCTTC 1 cut(s) 921
BstBAI YACGTR 1 cut(s) 130
BstC8I GCNNGC 2 cut(s) 9, 939
BstDEI CTNAG 7 cut(s) 222, 330, 613, 750, 947, 1085, 1172
BstDSI CCRYGG 1 cut(s) 996
BstF5I GGATG 3 cut(s) 152, 760, 1289
BstKTI GATC 2 cut(s) 313, 583
BstMAI GTCTC 2 cut(s) 753, 1025
BstMBI GATC 2 cut(s) 310, 580
BstMCI CGRYCG 1 cut(s) 27
BstMWI GCNNNNNNNGC 3 cut(s) 23, 623, 632
BstNI CCWGG 1 cut(s) 263
BstNSI RCATGY 1 cut(s) 807
BstSCI CCNGG 2 cut(s) 261, 556
BstSFI CTRYAG 1 cut(s) 60
BstSLI GKGCMC 1 cut(s) 501
BstV1I GCAGC 4 cut(s) 40, 304, 562, 613
BstX2I RGATCY 1 cut(s) 310
BstYI RGATCY 1 cut(s) 310
BstZI CGGCCG 1 cut(s) 24
Bsu15I ATCGAT 1 cut(s) 677
BsuRI GGCC 2 cut(s) 26, 419
BsuTUI ATCGAT 1 cut(s) 677
BtgI CCRYGG 1 cut(s) 996
BtsCI GGATG 3 cut(s) 152, 760, 1289
BtsIMutI CAGTG 3 cut(s) 11, 327, 386
Cac8I GCNNGC 2 cut(s) 9, 939
Cfr13I GGNCC 1 cut(s) 1176
ClaI ATCGAT 1 cut(s) 677
Csp6I GTAC 2 cut(s) 284, 696
CviAII CATG 6 cut(s) 415, 804, 815, 997, 1150, 1192
CviQI GTAC 2 cut(s) 284, 696
DdeI CTNAG 7 cut(s) 222, 330, 613, 750, 947, 1085, 1172
DpnI GATC 2 cut(s) 312, 582
DpnII GATC 2 cut(s) 310, 580
DraI TTTAAA 2 cut(s) 1062, 1224
EaeI YGGCCR 2 cut(s) 24, 417
EagI CGGCCG 1 cut(s) 24
Eam1104I CTCTTC 1 cut(s) 921
EarI CTCTTC 1 cut(s) 921
EclXI CGGCCG 1 cut(s) 24
Eco130I CCWWGG 1 cut(s) 996
Eco47I GGWCC 1 cut(s) 1176
Eco52I CGGCCG 1 cut(s) 24
EcoO109I RGGNCCY 1 cut(s) 1176
EcoRII CCWGG 1 cut(s) 261
EcoT14I CCWWGG 1 cut(s) 996
EcoT22I ATGCAT 2 cut(s) 199, 1197
ErhI CCWWGG 1 cut(s) 996
FaeI CATG 6 cut(s) 418, 807, 818, 1000, 1153, 1195
FaqI GGGAC 3 cut(s) 355, 623, 1271
FatI CATG 6 cut(s) 414, 803, 814, 996, 1149, 1191
Fnu4HI GCNGC 4 cut(s) 54, 318, 551, 627
FokI GGATG 3 cut(s) 159, 767, 1276
Fsp4HI GCNGC 4 cut(s) 54, 318, 551, 627
FspBI CTAG 3 cut(s) 321, 630, 1283
GluI GCNGC 4 cut(s) 54, 318, 551, 627
HaeIII GGCC 2 cut(s) 26, 419
HapII CCGG 2 cut(s) 83, 557
Hin1II CATG 6 cut(s) 418, 807, 818, 1000, 1153, 1195
HindIII AAGCTT 3 cut(s) 267, 1046, 1158
HinfI GANTC 3 cut(s) 469, 611, 1244
HpaII CCGG 2 cut(s) 83, 557
HphI GGTGA 2 cut(s) 439, 1150
Hpy166II GTNNAC 2 cut(s) 408, 499
Hpy188I TCNGA 4 cut(s) 735, 920, 1249, 1308
Hpy188III TCNNGA 7 cut(s) 83, 473, 689, 752, 776, 905, 1024
Hpy8I GTNNAC 2 cut(s) 408, 499
HpyAV CCTTC 2 cut(s) 108, 1138
HpyCH4III ACNGT 5 cut(s) 64, 390, 412, 593, 1220
HpyCH4IV ACGT 1 cut(s) 129
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 623, 632
HpyF3I CTNAG 7 cut(s) 222, 330, 613, 750, 947, 1085, 1172
HpySE526I ACGT 1 cut(s) 129
Hsp92II CATG 6 cut(s) 418, 807, 818, 1000, 1153, 1195
Kpn2I TCCGGA 1 cut(s) 82
Kzo9I GATC 2 cut(s) 310, 580
LmnI GCTCC 1 cut(s) 717
Lsp1109I GCAGC 4 cut(s) 40, 304, 562, 613
LweI GCATC 3 cut(s) 65, 211, 496
MaeI CTAG 3 cut(s) 321, 630, 1283
MaeII ACGT 1 cut(s) 129
MaeIII GTNAC 7 cut(s) 254, 390, 566, 587, 764, 806, 853
MalI GATC 2 cut(s) 312, 582
MboI GATC 2 cut(s) 310, 580
MboII GAAGA 5 cut(s) 193, 673, 890, 938, 1179
MflI RGATCY 1 cut(s) 310
MhlI GDGCHC 4 cut(s) 13, 501, 637, 837
MlsI TGGCCA 1 cut(s) 419
MluNI TGGCCA 1 cut(s) 419
MlyI GAGTC 1 cut(s) 605
MmeI TCCRAC 1 cut(s) 750
MnlI CCTC 8 cut(s) 79, 219, 325, 512, 922, 981, 1129, 1189
Mox20I TGGCCA 1 cut(s) 419
Mph1103I ATGCAT 2 cut(s) 199, 1197
MroI TCCGGA 1 cut(s) 82
MroXI GAANNNNTTC 1 cut(s) 188
MscI TGGCCA 1 cut(s) 419
MseI TTAA 5 cut(s) 153, 1061, 1130, 1223, 1230
MslI CAYNNNNRTG 5 cut(s) 399, 413, 504, 963, 995
Msp20I TGGCCA 1 cut(s) 419
MspI CCGG 2 cut(s) 83, 557
MspR9I CCNGG 2 cut(s) 263, 558
MvaI CCWGG 1 cut(s) 263
MwoI GCNNNNNNNGC 3 cut(s) 23, 623, 632
NciI CCSGG 1 cut(s) 558
NcoI CCATGG 1 cut(s) 996
NdeII GATC 2 cut(s) 310, 580
NlaIII CATG 6 cut(s) 418, 807, 818, 1000, 1153, 1195
NlaIV GGNNCC 1 cut(s) 713
NmuCI GTSAC 2 cut(s) 566, 587
NsiI ATGCAT 2 cut(s) 199, 1197
NspI RCATGY 1 cut(s) 807
OliI CACNNNNGTG 1 cut(s) 399
PdmI GAANNNNTTC 1 cut(s) 188
PfeI GAWTC 2 cut(s) 469, 1244
PkrI GCNGC 4 cut(s) 55, 319, 552, 628
PleI GAGTC 1 cut(s) 605
PpsI GAGTC 1 cut(s) 605
Ppu21I YACGTR 1 cut(s) 130
PpuMI RGGWCCY 1 cut(s) 1176
Psp5II RGGWCCY 1 cut(s) 1176
Psp6I CCWGG 1 cut(s) 261
PspGI CCWGG 1 cut(s) 261
PspN4I GGNNCC 1 cut(s) 713
PspPI GGNCC 1 cut(s) 1176
PspPPI RGGWCCY 1 cut(s) 1176
PsuI RGATCY 1 cut(s) 310
RsaI GTAC 2 cut(s) 285, 697
RsaNI GTAC 2 cut(s) 284, 696
RseI CAYNNNNRTG 5 cut(s) 399, 413, 504, 963, 995
SaqAI TTAA 5 cut(s) 153, 1061, 1130, 1223, 1230
SatI GCNGC 4 cut(s) 54, 318, 551, 627
Sau3AI GATC 2 cut(s) 310, 580
Sau96I GGNCC 1 cut(s) 1176
SchI GAGTC 1 cut(s) 605
ScrFI CCNGG 2 cut(s) 263, 558
SduI GDGCHC 4 cut(s) 13, 501, 637, 837
SfaNI GCATC 3 cut(s) 65, 211, 496
SfcI CTRYAG 1 cut(s) 60
SinI GGWCC 1 cut(s) 1176
SmiMI CAYNNNNRTG 5 cut(s) 399, 413, 504, 963, 995
SspI AATATT 1 cut(s) 793
SspMI CTAG 3 cut(s) 321, 630, 1283
StyD4I CCNGG 2 cut(s) 261, 556
StyI CCWWGG 1 cut(s) 996
TaaI ACNGT 5 cut(s) 64, 390, 412, 593, 1220
TaiI ACGT 1 cut(s) 132
TaqI TCGA 4 cut(s) 41, 461, 504, 677
TatI WGTACW 1 cut(s) 283
TfiI GAWTC 2 cut(s) 469, 1244
Tru1I TTAA 5 cut(s) 153, 1061, 1130, 1223, 1230
Tru9I TTAA 5 cut(s) 153, 1061, 1130, 1223, 1230
TscAI CASTG 3 cut(s) 18, 334, 393
TseFI GTSAC 2 cut(s) 566, 587
TseI GCWGC 4 cut(s) 53, 317, 550, 626
Tsp45I GTSAC 2 cut(s) 566, 587
TspDTI ATGAA 1 cut(s) 1157
TspGWI ACGGA 1 cut(s) 704
TspRI CASTG 3 cut(s) 18, 334, 393
VneI GTGCAC 1 cut(s) 497
VpaK11BI GGWCC 1 cut(s) 1176
XapI RAATTY 6 cut(s) 92, 140, 173, 187, 1027, 1063
XceI RCATGY 1 cut(s) 807
XmnI GAANNNNTTC 1 cut(s) 188
XspI CTAG 3 cut(s) 321, 630, 1283
Zsp2I ATGCAT 2 cut(s) 199, 1197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.