pycom05g02320

IST1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0015717
Physical Location & Seq
Reverse (-)
2666148 .. 2667108
961 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g02320.1

Sequence Viewer

Length: 525 bp
ATGGCCACAACGGTAGAGTTGCTACCAGCCTTGATGTGTTCTGAGGCAGCTGAATTGATCGATGTAGGTCACCCAAAACTTCAACACCTTGCGACGCAAGACGGAGACGGCAACCCACTGCAAATTCCATCGACAGTCCACGACCATTCCATTCCGCCATTGCTCTCATCTTCACCGATGTTGCAAGCACTGGCAACTTCGGCCGTCATGCCACACTCGAACAACTCCGCTACATCTACAGTCTTGCTTCCAGAATCGAAGACGATGACCTCTGCTCCGCAGCTACTCCATAAGCACTCGTTTTCCGATACTACGAAAACTGGACCTGAGGTAGTTCTTAATTTGAGTATCATTTTAGCAAATTCGGAGGCTAAATTCCTTTCTCTTTTGCATGATAACTATATTGGATGGGTTAGTTGGTGCTATGAGATTTCTGAACTGATTGCAGTAAGGAATATTTTTGAGGAAATTATGGTAAAGATTTTGTATATGCAGCTGTTGATGTACGTCCCAGCTGTGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

175

Amino Acids

19.02

Weight (kDa)

5.16

Isoelectric Point (pI)

50.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 155, 228, 278
AcoI YGGCCR 2 cut(s) 3, 201
AcsI RAATTY 3 cut(s) 123, 361, 374
AfaI GTAC 1 cut(s) 506
AgsI TTSAA 1 cut(s) 83
AluBI AGCT 4 cut(s) 50, 283, 496, 515
AluI AGCT 4 cut(s) 50, 283, 496, 515
Alw26I GTCTC 1 cut(s) 99
AoxI GGCC 2 cut(s) 3, 201
ApeKI GCWGC 3 cut(s) 47, 280, 493
ApoI RAATTY 3 cut(s) 123, 361, 374
AspS9I GGNCC 1 cut(s) 323
AsuHPI GGTGA 2 cut(s) 62, 165
AvaII GGWCC 1 cut(s) 323
AxyI CCTNAGG 1 cut(s) 327
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 266
BbvI GCAGC 3 cut(s) 59, 292, 505
BccI CCATC 2 cut(s) 136, 402
BceAI ACGGC 2 cut(s) 124, 188
BcoDI GTCTC 1 cut(s) 99
BfmI CTRYAG 1 cut(s) 237
BisI GCNGC 3 cut(s) 48, 281, 494
BlsI GCNGC 3 cut(s) 49, 282, 495
Bme18I GGWCC 1 cut(s) 323
BmgT120I GGNCC 1 cut(s) 323
BpiI GAAGAC 1 cut(s) 266
Bsa29I ATCGAT 1 cut(s) 60
BsaXI ACNNNNNCTCC 2 cut(s) 259, 289
Bse1I ACTGG 2 cut(s) 195, 325
Bse21I CCTNAGG 1 cut(s) 327
Bse3DI GCAATG 1 cut(s) 158
BseCI ATCGAT 1 cut(s) 60
BseGI GGATG 1 cut(s) 413
BseMI GCAATG 1 cut(s) 158
BseMII CTCAG 2 cut(s) 33, 318
BseNI ACTGG 2 cut(s) 195, 325
BseX3I CGGCCG 1 cut(s) 201
BseXI GCAGC 3 cut(s) 59, 292, 505
BseYI CCCAGC 1 cut(s) 511
Bsh1285I CGRYCG 1 cut(s) 204
BshFI GGCC 2 cut(s) 5, 203
BshVI ATCGAT 1 cut(s) 60
BsiEI CGRYCG 1 cut(s) 204
BslFI GGGAC 1 cut(s) 494
BsmAI GTCTC 1 cut(s) 99
BsmBI CGTCTC 1 cut(s) 99
BsmFI GGGAC 1 cut(s) 494
BsnI GGCC 2 cut(s) 5, 203
Bsp143I GATC 1 cut(s) 57
BspACI CCGC 3 cut(s) 155, 228, 278
BspANI GGCC 2 cut(s) 5, 203
BspCNI CTCAG 2 cut(s) 34, 319
BspDI ATCGAT 1 cut(s) 60
BsrDI GCAATG 1 cut(s) 158
BsrI ACTGG 2 cut(s) 195, 325
BssMI GATC 1 cut(s) 57
Bst4CI ACNGT 3 cut(s) 13, 136, 241
BstC8I GCNNGC 1 cut(s) 186
BstDEI CTNAG 2 cut(s) 42, 327
BstEII GGTNACC 1 cut(s) 68
BstF5I GGATG 1 cut(s) 413
BstKTI GATC 1 cut(s) 60
BstMAI GTCTC 1 cut(s) 99
BstMBI GATC 1 cut(s) 57
BstMCI CGRYCG 1 cut(s) 204
BstMWI GCNNNNNNNGC 1 cut(s) 200
BstPI GGTNACC 1 cut(s) 68
BstSFI CTRYAG 1 cut(s) 237
BstV1I GCAGC 3 cut(s) 59, 292, 505
BstV2I GAAGAC 1 cut(s) 266
BstZI CGGCCG 1 cut(s) 201
Bsu15I ATCGAT 1 cut(s) 60
Bsu36I CCTNAGG 1 cut(s) 327
BsuRI GGCC 2 cut(s) 5, 203
BsuTUI ATCGAT 1 cut(s) 60
BtsCI GGATG 1 cut(s) 413
BtsI GCAGTG 1 cut(s) 116
BtsIMutI CAGTG 2 cut(s) 116, 188
Cac8I GCNNGC 1 cut(s) 186
Cfr13I GGNCC 1 cut(s) 323
ClaI ATCGAT 1 cut(s) 60
CseI GACGC 1 cut(s) 103
Csp6I GTAC 1 cut(s) 505
CviAII CATG 2 cut(s) 208, 392
CviJI RGCY 8 cut(s) 5, 29, 50, 203, 283, 371, 496, 515
CviKI_1 RGCY 8 cut(s) 5, 29, 50, 203, 283, 371, 496, 515
CviQI GTAC 1 cut(s) 505
DdeI CTNAG 2 cut(s) 42, 327
DpnI GATC 1 cut(s) 59
DpnII GATC 1 cut(s) 57
EaeI YGGCCR 2 cut(s) 3, 201
EagI CGGCCG 1 cut(s) 201
EciI GGCGGA 1 cut(s) 144
EclXI CGGCCG 1 cut(s) 201
Eco47I GGWCC 1 cut(s) 323
Eco52I CGGCCG 1 cut(s) 201
Eco81I CCTNAGG 1 cut(s) 327
Eco91I GGTNACC 1 cut(s) 68
EcoO65I GGTNACC 1 cut(s) 68
Esp3I CGTCTC 1 cut(s) 99
FaeI CATG 2 cut(s) 211, 395
FaiI YATR 8 cut(s) 209, 291, 393, 402, 426, 473, 489, 491
FaqI GGGAC 1 cut(s) 494
FatI CATG 2 cut(s) 207, 391
Fnu4HI GCNGC 3 cut(s) 48, 281, 494
FokI GGATG 1 cut(s) 420
Fsp4HI GCNGC 3 cut(s) 48, 281, 494
GluI GCNGC 3 cut(s) 48, 281, 494
GsaI CCCAGC 1 cut(s) 515
HaeIII GGCC 2 cut(s) 5, 203
HgaI GACGC 1 cut(s) 103
Hin1II CATG 2 cut(s) 211, 395
HinfI GANTC 1 cut(s) 254
HphI GGTGA 2 cut(s) 62, 165
Hpy166II GTNNAC 1 cut(s) 139
Hpy188I TCNGA 4 cut(s) 43, 307, 367, 436
Hpy188III TCNNGA 1 cut(s) 251
Hpy8I GTNNAC 1 cut(s) 139
Hpy99I CGWCG 1 cut(s) 97
HpyCH4III ACNGT 3 cut(s) 13, 136, 241
HpyCH4IV ACGT 1 cut(s) 507
HpyCH4V TGCA 5 cut(s) 121, 184, 391, 446, 493
HpyF10VI GCNNNNNNNGC 1 cut(s) 200
HpyF3I CTNAG 2 cut(s) 42, 327
HpySE526I ACGT 1 cut(s) 507
Hsp92II CATG 2 cut(s) 211, 395
Kzo9I GATC 1 cut(s) 57
LmnI GCTCC 1 cut(s) 280
LpnPI CCDG 5 cut(s) 39, 176, 264, 306, 339
Lsp1109I GCAGC 3 cut(s) 59, 292, 505
MaeII ACGT 1 cut(s) 507
MaeIII GTNAC 1 cut(s) 68
MalI GATC 1 cut(s) 59
MboI GATC 1 cut(s) 57
MboII GAAGA 2 cut(s) 162, 271
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 53, 123, 340, 361, 374, 468
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 5 cut(s) 37, 280, 322, 361, 457
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 339
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 3 cut(s) 50, 496, 515
MwoI GCNNNNNNNGC 1 cut(s) 200
NdeII GATC 1 cut(s) 57
NlaIII CATG 2 cut(s) 211, 395
NmuCI GTSAC 1 cut(s) 68
PfeI GAWTC 1 cut(s) 254
PkrI GCNGC 3 cut(s) 49, 282, 495
PspEI GGTNACC 1 cut(s) 68
PspFI CCCAGC 1 cut(s) 511
PspPI GGNCC 1 cut(s) 323
PvuII CAGCTG 3 cut(s) 50, 496, 515
RsaI GTAC 1 cut(s) 506
RsaNI GTAC 1 cut(s) 505
SaqAI TTAA 1 cut(s) 339
SatI GCNGC 3 cut(s) 48, 281, 494
Sau3AI GATC 1 cut(s) 57
Sau96I GGNCC 1 cut(s) 323
SfcI CTRYAG 1 cut(s) 237
SinI GGWCC 1 cut(s) 323
Sse9I AATT 6 cut(s) 53, 123, 340, 361, 374, 468
SsiI CCGC 3 cut(s) 155, 228, 278
SspI AATATT 1 cut(s) 457
TaaI ACNGT 3 cut(s) 13, 136, 241
TaiI ACGT 1 cut(s) 510
TaqI TCGA 4 cut(s) 60, 131, 218, 257
TasI AATT 6 cut(s) 53, 123, 340, 361, 374, 468
TfiI GAWTC 1 cut(s) 254
Tru1I TTAA 1 cut(s) 339
Tru9I TTAA 1 cut(s) 339
TscAI CASTG 2 cut(s) 123, 195
TseFI GTSAC 1 cut(s) 68
TseI GCWGC 3 cut(s) 47, 280, 493
Tsp45I GTSAC 1 cut(s) 68
TspGWI ACGGA 1 cut(s) 117
TspRI CASTG 2 cut(s) 123, 195
VpaK11BI GGWCC 1 cut(s) 323
XapI RAATTY 3 cut(s) 123, 361, 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.