pycom08g13400

IST1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0015717
Physical Location & Seq
Forward (+)
12196896 .. 12197081
186 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g13400.1

Sequence Viewer

Length: 186 bp
ATGGTATCATCCTTCTTGAATCCTGGTTTTCTTCTTGTGCACGCCCACCTGACCCGTCTTCCTGCCATGGATGCGTGTATCTCAGTTTCAATTGACTCTTTCGAAGCTTCGATGTGTGCTGTGGTCCGCGAAGCTATGGATTCTACACGCGACACCATTCACAAGGCTGTGAATCCTGTACTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

62

Amino Acids

6.62

Weight (kDa)

5.76

Isoelectric Point (pI)

37.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 129, 150
AciI CCGC 1 cut(s) 127
AfaI GTAC 1 cut(s) 180
AgsI TTSAA 2 cut(s) 19, 90
AjnI CCWGG 1 cut(s) 22
AluBI AGCT 2 cut(s) 107, 134
AluI AGCT 2 cut(s) 107, 134
Alw21I GWGCWC 1 cut(s) 42
Alw44I GTGCAC 1 cut(s) 38
ApaLI GTGCAC 1 cut(s) 38
AspS9I GGNCC 1 cut(s) 124
AsuII TTCGAA 1 cut(s) 102
AvaII GGWCC 1 cut(s) 124
BaeGI GKGCMC 1 cut(s) 42
BbsI GAAGAC 1 cut(s) 50
Bbv12I GWGCWC 1 cut(s) 42
BciT130I CCWGG 1 cut(s) 24
BfmI CTRYAG 1 cut(s) 182
Bme1390I CCNGG 1 cut(s) 24
Bme18I GGWCC 1 cut(s) 124
BmgT120I GGNCC 1 cut(s) 124
BmrFI CCNGG 1 cut(s) 24
BmsI GCATC 1 cut(s) 61
BpiI GAAGAC 1 cut(s) 50
Bpu14I TTCGAA 1 cut(s) 102
BsaJI CCNNGG 1 cut(s) 66
BseBI CCWGG 1 cut(s) 24
BseDI CCNNGG 1 cut(s) 66
BseGI GGATG 2 cut(s) 8, 76
BseMII CTCAG 1 cut(s) 96
BseSI GKGCMC 1 cut(s) 42
Bsh1236I CGCG 2 cut(s) 129, 150
BsiHKAI GWGCWC 1 cut(s) 42
Bsp119I TTCGAA 1 cut(s) 102
Bsp1286I GDGCHC 1 cut(s) 42
Bsp19I CCATGG 1 cut(s) 66
BspACI CCGC 1 cut(s) 127
BspCNI CTCAG 1 cut(s) 95
BspFNI CGCG 2 cut(s) 129, 150
BspT104I TTCGAA 1 cut(s) 102
BssECI CCNNGG 1 cut(s) 66
BssT1I CCWWGG 1 cut(s) 66
Bst2UI CCWGG 1 cut(s) 24
Bst4CI ACNGT 1 cut(s) 183
BstBI TTCGAA 1 cut(s) 102
BstC8I GCNNGC 1 cut(s) 42
BstDEI CTNAG 1 cut(s) 82
BstDSI CCRYGG 1 cut(s) 66
BstF5I GGATG 2 cut(s) 8, 76
BstFNI CGCG 2 cut(s) 129, 150
BstMWI GCNNNNNNNGC 1 cut(s) 71
BstNI CCWGG 1 cut(s) 24
BstSCI CCNGG 1 cut(s) 22
BstSFI CTRYAG 1 cut(s) 182
BstSLI GKGCMC 1 cut(s) 42
BstUI CGCG 2 cut(s) 129, 150
BstV2I GAAGAC 1 cut(s) 50
BtgI CCRYGG 1 cut(s) 66
BtsCI GGATG 2 cut(s) 8, 76
Cac8I GCNNGC 1 cut(s) 42
Cfr13I GGNCC 1 cut(s) 124
Csp6I GTAC 1 cut(s) 179
CviAII CATG 1 cut(s) 67
CviJI RGCY 3 cut(s) 107, 134, 167
CviKI_1 RGCY 3 cut(s) 107, 134, 167
CviQI GTAC 1 cut(s) 179
DdeI CTNAG 1 cut(s) 82
Eco130I CCWWGG 1 cut(s) 66
Eco47I GGWCC 1 cut(s) 124
EcoRII CCWGG 1 cut(s) 22
EcoT14I CCWWGG 1 cut(s) 66
ErhI CCWWGG 1 cut(s) 66
FaeI CATG 1 cut(s) 70
FaiI YATR 2 cut(s) 68, 137
FatI CATG 1 cut(s) 66
FokI GGATG 1 cut(s) 83
Hin1II CATG 1 cut(s) 70
HindIII AAGCTT 1 cut(s) 105
HinfI GANTC 4 cut(s) 19, 95, 140, 172
Hpy166II GTNNAC 1 cut(s) 40
Hpy188III TCNNGA 1 cut(s) 16
Hpy8I GTNNAC 1 cut(s) 40
HpyAV CCTTC 1 cut(s) 22
HpyCH4III ACNGT 1 cut(s) 183
HpyCH4V TGCA 1 cut(s) 40
HpyF10VI GCNNNNNNNGC 1 cut(s) 71
HpyF3I CTNAG 1 cut(s) 82
Hsp92II CATG 1 cut(s) 70
LpnPI CCDG 4 cut(s) 9, 36, 62, 75
LweI GCATC 1 cut(s) 61
MboII GAAGA 2 cut(s) 23, 50
MfeI CAATTG 1 cut(s) 90
MhlI GDGCHC 1 cut(s) 42
MluCI AATT 1 cut(s) 90
MlyI GAGTC 1 cut(s) 89
MspR9I CCNGG 1 cut(s) 24
MunI CAATTG 1 cut(s) 90
MvaI CCWGG 1 cut(s) 24
MvnI CGCG 2 cut(s) 129, 150
MwoI GCNNNNNNNGC 1 cut(s) 71
NcoI CCATGG 1 cut(s) 66
NlaIII CATG 1 cut(s) 70
NspV TTCGAA 1 cut(s) 102
PfeI GAWTC 3 cut(s) 19, 140, 172
PleI GAGTC 1 cut(s) 89
PpsI GAGTC 1 cut(s) 89
Psp6I CCWGG 1 cut(s) 22
PspGI CCWGG 1 cut(s) 22
PspPI GGNCC 1 cut(s) 124
RsaI GTAC 1 cut(s) 180
RsaNI GTAC 1 cut(s) 179
Sau96I GGNCC 1 cut(s) 124
SchI GAGTC 1 cut(s) 89
ScrFI CCNGG 1 cut(s) 24
SduI GDGCHC 1 cut(s) 42
SetI ASST 3 cut(s) 51, 109, 136
SfaNI GCATC 1 cut(s) 61
SfcI CTRYAG 1 cut(s) 182
SfuI TTCGAA 1 cut(s) 102
SinI GGWCC 1 cut(s) 124
Sse9I AATT 1 cut(s) 90
SsiI CCGC 1 cut(s) 127
StyD4I CCNGG 1 cut(s) 22
StyI CCWWGG 1 cut(s) 66
TaaI ACNGT 1 cut(s) 183
TaqI TCGA 2 cut(s) 102, 110
TasI AATT 1 cut(s) 90
TatI WGTACW 1 cut(s) 178
TfiI GAWTC 3 cut(s) 19, 140, 172
VneI GTGCAC 1 cut(s) 38
VpaK11BI GGWCC 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.