pycom09g17550

IST1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0015717
Physical Location & Seq
Forward (+)
18047740 .. 18048396
657 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g17550.1

Sequence Viewer

Length: 657 bp
ATGGTATCATCCTTCTCGAATCCTGGTTTCCCTATTGTGCATACCCACCCGACCCGTCTTCCTGCCATGGATGCACGCCTCTCCGCTGCAATTGACTCATTTGAAGCTTCGATGCATGCTGCAATCCGTGATGTGATCTGCGAAGCTATGGATTCTGCACTCACCGTTATTCACAAGGCTGTGAATCCTGTACTCGTAGAAATCTGCAGTGACCTTGCTCAGTTACGTCGTGACTTGGCTCTCGATGTCGAGGGAGATTCTGATCCAGCCTCAATGTGTTCTAAGGAAGCTGAATCGATCGAGGTAGGTCACCAGAAACTTCAAAAACTTGAGACGCATGATGGAGACGGCAACCTATTGCGAATTCCATCAACAATCCACGACCATTCCATTCCGCCATTGCTCTTATCGTCACCGATGTTGAGAGCACTTGCAAGTTCTCCGGTCAAGTCCCAATTAAAAGAGTCTGTGGTAGCCACAATTCCACTGCAACATTTTGAGAAACCCATGCTTATTGCGATGGTGACCATAATGTCGTTCCCATTTTCAGTAATCGTTGGCAAAACTGAGGCCTTATCTCAACTGGCAGACCAGGACGCCAATGTTACCACCCACATTGAGGGGTGTTGTGACATCCCGTCCCAAAATTCATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

219

Amino Acids

23.48

Weight (kDa)

5.13

Isoelectric Point (pI)

52.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 532
AciI CCGC 2 cut(s) 84, 395
AclWI GGATC 1 cut(s) 257
AcsI RAATTY 2 cut(s) 363, 646
AcyI GRCGYC 1 cut(s) 597
AfaI GTAC 1 cut(s) 192
AfiI CCNNNNNNNGG 1 cut(s) 619
AgsI TTSAA 2 cut(s) 104, 323
AhdI GACNNNNNGTC 1 cut(s) 637
AjnI CCWGG 2 cut(s) 22, 591
AluBI AGCT 3 cut(s) 107, 146, 290
AluI AGCT 3 cut(s) 107, 146, 290
Alw21I GWGCWC 1 cut(s) 430
Alw26I GTCTC 2 cut(s) 326, 339
AlwI GGATC 1 cut(s) 257
AoxI GGCC 1 cut(s) 570
ApeKI GCWGC 2 cut(s) 86, 119
ApoI RAATTY 2 cut(s) 363, 646
AsuHPI GGTGA 4 cut(s) 154, 302, 405, 535
BbsI GAAGAC 1 cut(s) 50
Bbv12I GWGCWC 1 cut(s) 430
BbvI GCAGC 2 cut(s) 73, 106
BccI CCATC 3 cut(s) 335, 376, 514
BceAI ACGGC 1 cut(s) 364
BciT130I CCWGG 2 cut(s) 24, 593
BcoDI GTCTC 2 cut(s) 326, 339
BfmI CTRYAG 1 cut(s) 205
BisI GCNGC 2 cut(s) 87, 120
BlsI GCNGC 2 cut(s) 88, 121
Bme1390I CCNGG 2 cut(s) 24, 593
BmeRI GACNNNNNGTC 1 cut(s) 637
BmrFI CCNGG 2 cut(s) 24, 593
BmsI GCATC 2 cut(s) 61, 102
BpiI GAAGAC 1 cut(s) 50
BpuEI CTTGAG 1 cut(s) 350
Bsa29I ATCGAT 1 cut(s) 296
BsaBI GATNNNNATC 1 cut(s) 261
BsaHI GRCGYC 1 cut(s) 597
BsaJI CCNNGG 1 cut(s) 66
BsaWI WCCGGW 1 cut(s) 442
Bsc4I CCNNNNNNNGG 1 cut(s) 619
Bse1I ACTGG 1 cut(s) 588
Bse3DI GCAATG 1 cut(s) 398
Bse8I GATNNNNATC 1 cut(s) 261
BseBI CCWGG 2 cut(s) 24, 593
BseCI ATCGAT 1 cut(s) 296
BseDI CCNNGG 1 cut(s) 66
BseGI GGATG 3 cut(s) 8, 76, 633
BseJI GATNNNNATC 1 cut(s) 261
BseLI CCNNNNNNNGG 1 cut(s) 619
BseMI GCAATG 1 cut(s) 398
BseMII CTCAG 2 cut(s) 233, 558
BseNI ACTGG 1 cut(s) 588
BseXI GCAGC 2 cut(s) 73, 106
BsgI GTGCAG 1 cut(s) 141
Bsh1285I CGRYCG 1 cut(s) 300
BshFI GGCC 1 cut(s) 572
BshVI ATCGAT 1 cut(s) 296
BsiEI CGRYCG 1 cut(s) 300
BsiHKAI GWGCWC 1 cut(s) 430
BsiSI CCGG 1 cut(s) 443
BslFI GGGAC 2 cut(s) 436, 625
BslI CCNNNNNNNGG 1 cut(s) 619
BsmAI GTCTC 2 cut(s) 326, 339
BsmBI CGTCTC 2 cut(s) 326, 339
BsmFI GGGAC 2 cut(s) 436, 625
BsnI GGCC 1 cut(s) 572
Bsp1286I GDGCHC 1 cut(s) 430
Bsp143I GATC 3 cut(s) 135, 262, 297
Bsp19I CCATGG 1 cut(s) 66
BspACI CCGC 2 cut(s) 84, 395
BspANI GGCC 1 cut(s) 572
BspCNI CTCAG 2 cut(s) 232, 559
BspDI ATCGAT 1 cut(s) 296
BspMAI CTGCAG 1 cut(s) 209
BspPI GGATC 1 cut(s) 257
BsrDI GCAATG 1 cut(s) 398
BsrI ACTGG 1 cut(s) 588
BssECI CCNNGG 1 cut(s) 66
BssMI GATC 3 cut(s) 135, 262, 297
BssNI GRCGYC 1 cut(s) 597
BssT1I CCWWGG 1 cut(s) 66
Bst2UI CCWGG 2 cut(s) 24, 593
Bst4CI ACNGT 1 cut(s) 166
BstACI GRCGYC 1 cut(s) 597
BstC8I GCNNGC 2 cut(s) 76, 117
BstDEI CTNAG 3 cut(s) 219, 282, 567
BstDSI CCRYGG 1 cut(s) 66
BstEII GGTNACC 2 cut(s) 308, 523
BstF5I GGATG 3 cut(s) 8, 76, 633
BstKTI GATC 3 cut(s) 138, 265, 300
BstMAI GTCTC 2 cut(s) 326, 339
BstMBI GATC 3 cut(s) 135, 262, 297
BstMCI CGRYCG 1 cut(s) 300
BstMWI GCNNNNNNNGC 1 cut(s) 71
BstNI CCWGG 2 cut(s) 24, 593
BstNSI RCATGY 1 cut(s) 119
BstPI GGTNACC 2 cut(s) 308, 523
BstSCI CCNGG 2 cut(s) 22, 591
BstSFI CTRYAG 1 cut(s) 205
BstV1I GCAGC 2 cut(s) 73, 106
BstV2I GAAGAC 1 cut(s) 50
Bsu15I ATCGAT 1 cut(s) 296
BsuRI GGCC 1 cut(s) 572
BsuTUI ATCGAT 1 cut(s) 296
BtgI CCRYGG 1 cut(s) 66
BtgZI GCGATG 1 cut(s) 533
BtsCI GGATG 3 cut(s) 8, 76, 633
BtsI GCAGTG 2 cut(s) 214, 485
BtsIMutI CAGTG 2 cut(s) 214, 485
Cac8I GCNNGC 2 cut(s) 76, 117
ClaI ATCGAT 1 cut(s) 296
CseI GACGC 2 cut(s) 343, 605
Csp6I GTAC 1 cut(s) 191
CspCI CAANNNNNGTGG 2 cut(s) 598, 633
CviAII CATG 5 cut(s) 67, 116, 338, 508, 651
CviJI RGCY 8 cut(s) 107, 146, 179, 239, 269, 290, 476, 572
CviKI_1 RGCY 8 cut(s) 107, 146, 179, 239, 269, 290, 476, 572
CviQI GTAC 1 cut(s) 191
DdeI CTNAG 3 cut(s) 219, 282, 567
DpnI GATC 3 cut(s) 137, 264, 299
DpnII GATC 3 cut(s) 135, 262, 297
DrdI GACNNNNNNGTC 1 cut(s) 532
DriI GACNNNNNGTC 1 cut(s) 637
DseDI GACNNNNNNGTC 1 cut(s) 532
Eam1105I GACNNNNNGTC 1 cut(s) 637
EciI GGCGGA 1 cut(s) 384
Eco130I CCWWGG 1 cut(s) 66
Eco147I AGGCCT 1 cut(s) 572
Eco91I GGTNACC 2 cut(s) 308, 523
EcoO65I GGTNACC 2 cut(s) 308, 523
EcoRI GAATTC 1 cut(s) 363
EcoRII CCWGG 2 cut(s) 22, 591
EcoT14I CCWWGG 1 cut(s) 66
EcoT22I ATGCAT 1 cut(s) 117
ErhI CCWWGG 1 cut(s) 66
Esp3I CGTCTC 2 cut(s) 326, 339
FaeI CATG 5 cut(s) 70, 119, 341, 511, 654
FaiI YATR 8 cut(s) 42, 68, 117, 149, 339, 509, 530, 652
FaqI GGGAC 2 cut(s) 436, 625
FatI CATG 5 cut(s) 66, 115, 337, 507, 650
Fnu4HI GCNGC 2 cut(s) 87, 120
FokI GGATG 2 cut(s) 83, 620
Fsp4HI GCNGC 2 cut(s) 87, 120
GluI GCNGC 2 cut(s) 87, 120
HaeIII GGCC 1 cut(s) 572
HapII CCGG 1 cut(s) 443
HgaI GACGC 2 cut(s) 343, 605
Hin1I GRCGYC 1 cut(s) 597
Hin1II CATG 5 cut(s) 70, 119, 341, 511, 654
HindIII AAGCTT 1 cut(s) 105
HinfI GANTC 7 cut(s) 19, 95, 152, 184, 257, 293, 464
HpaII CCGG 1 cut(s) 443
HphI GGTGA 4 cut(s) 154, 302, 405, 535
Hpy188I TCNGA 1 cut(s) 262
Hpy188III TCNNGA 3 cut(s) 16, 230, 242
Hpy99I CGWCG 1 cut(s) 231
HpyAV CCTTC 1 cut(s) 22
HpyCH4III ACNGT 1 cut(s) 166
HpyCH4IV ACGT 1 cut(s) 226
HpyCH4V TGCA 9 cut(s) 40, 74, 89, 115, 122, 158, 207, 434, 490
HpyF10VI GCNNNNNNNGC 1 cut(s) 71
HpyF3I CTNAG 3 cut(s) 219, 282, 567
HpySE526I ACGT 1 cut(s) 226
Hsp92I GRCGYC 1 cut(s) 597
Hsp92II CATG 5 cut(s) 70, 119, 341, 511, 654
Kzo9I GATC 3 cut(s) 135, 262, 297
Lsp1109I GCAGC 2 cut(s) 73, 106
LweI GCATC 2 cut(s) 61, 102
MaeII ACGT 1 cut(s) 226
MaeIII GTNAC 8 cut(s) 209, 222, 230, 308, 411, 523, 604, 629
MalI GATC 3 cut(s) 137, 264, 299
MboI GATC 3 cut(s) 135, 262, 297
MboII GAAGA 1 cut(s) 50
MfeI CAATTG 1 cut(s) 90
MhlI GDGCHC 1 cut(s) 430
MluCI AATT 5 cut(s) 90, 363, 455, 480, 646
MlyI GAGTC 2 cut(s) 89, 473
MnlI CCTC 6 cut(s) 89, 244, 280, 295, 562, 613
Mph1103I ATGCAT 1 cut(s) 117
MseI TTAA 2 cut(s) 458, 655
MspA1I CMGCKG 1 cut(s) 86
MspI CCGG 1 cut(s) 443
MspR9I CCNGG 2 cut(s) 24, 593
MunI CAATTG 1 cut(s) 90
MvaI CCWGG 2 cut(s) 24, 593
MwoI GCNNNNNNNGC 1 cut(s) 71
NcoI CCATGG 1 cut(s) 66
NdeII GATC 3 cut(s) 135, 262, 297
NlaIII CATG 5 cut(s) 70, 119, 341, 511, 654
NmuCI GTSAC 6 cut(s) 209, 230, 308, 411, 523, 629
NsiI ATGCAT 1 cut(s) 117
NspI RCATGY 1 cut(s) 119
PaeI GCATGC 1 cut(s) 119
PceI AGGCCT 1 cut(s) 572
PfeI GAWTC 5 cut(s) 19, 152, 184, 257, 293
PkrI GCNGC 2 cut(s) 88, 121
Ple19I CGATCG 1 cut(s) 300
PleI GAGTC 2 cut(s) 89, 472
PpsI GAGTC 2 cut(s) 89, 472
Psp6I CCWGG 2 cut(s) 22, 591
PspEI GGTNACC 2 cut(s) 308, 523
PspGI CCWGG 2 cut(s) 22, 591
PstI CTGCAG 1 cut(s) 209
PvuI CGATCG 1 cut(s) 300
RsaI GTAC 1 cut(s) 192
RsaNI GTAC 1 cut(s) 191
SaqAI TTAA 2 cut(s) 458, 655
SatI GCNGC 2 cut(s) 87, 120
Sau3AI GATC 3 cut(s) 135, 262, 297
SchI GAGTC 2 cut(s) 89, 473
ScrFI CCNGG 2 cut(s) 24, 593
SduI GDGCHC 1 cut(s) 430
SetI ASST 8 cut(s) 109, 148, 216, 229, 292, 306, 310, 357
SfaNI GCATC 2 cut(s) 61, 102
SfcI CTRYAG 1 cut(s) 205
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
SphI GCATGC 1 cut(s) 119
Sse9I AATT 5 cut(s) 90, 363, 455, 480, 646
SseBI AGGCCT 1 cut(s) 572
SsiI CCGC 2 cut(s) 84, 395
StuI AGGCCT 1 cut(s) 572
StyD4I CCNGG 2 cut(s) 22, 591
StyI CCWWGG 1 cut(s) 66
TaaI ACNGT 1 cut(s) 166
TaiI ACGT 1 cut(s) 229
TaqI TCGA 6 cut(s) 17, 110, 243, 249, 296, 300
TasI AATT 5 cut(s) 90, 363, 455, 480, 646
TatI WGTACW 1 cut(s) 190
TfiI GAWTC 5 cut(s) 19, 152, 184, 257, 293
Tru1I TTAA 2 cut(s) 458, 655
Tru9I TTAA 2 cut(s) 458, 655
TscAI CASTG 2 cut(s) 214, 492
TseFI GTSAC 6 cut(s) 209, 230, 308, 411, 523, 629
TseI GCWGC 2 cut(s) 86, 119
Tsp45I GTSAC 6 cut(s) 209, 230, 308, 411, 523, 629
TspDTI ATGAA 1 cut(s) 639
TspGWI ACGGA 1 cut(s) 116
TspRI CASTG 2 cut(s) 214, 492
XapI RAATTY 2 cut(s) 363, 646
XceI RCATGY 1 cut(s) 119
Zsp2I ATGCAT 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.