pycom12494g00040

IST1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012494
OG0015717
Physical Location & Seq
Forward (+)
115917 .. 116141
225 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12494g00040.1

Sequence Viewer

Length: 225 bp
ATGGTATCATCCTTCTCGAATCCTGGTTTCCCTCTTGTGCACACCCACCCAACCCGTCTCCTTGCCATGGATGCGCGTCTCTCAGCTGCAATTGACTCCTTCGAAGTTTCGATGCGTGTTGCGGCCTGTGAAGCTATGGATTCTGTATGCGACGCCATTCACAAAGCTATGAATCCTGTGCTCGCAGAGATCCACTGTGAAATTGCTTGCTTTAATCCGCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

75

Amino Acids

7.99

Weight (kDa)

5.3

Isoelectric Point (pI)

48.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 76
AciI CCGC 2 cut(s) 122, 218
AclWI GGATC 1 cut(s) 184
AcyI GRCGYC 1 cut(s) 153
AfiI CCNNNNNNNGG 1 cut(s) 67
AjnI CCWGG 1 cut(s) 22
AluBI AGCT 3 cut(s) 86, 134, 167
AluI AGCT 3 cut(s) 86, 134, 167
Alw21I GWGCWC 2 cut(s) 42, 183
Alw26I GTCTC 2 cut(s) 62, 83
Alw44I GTGCAC 1 cut(s) 38
AlwI GGATC 1 cut(s) 184
AoxI GGCC 1 cut(s) 123
ApaLI GTGCAC 1 cut(s) 38
ApeKI GCWGC 1 cut(s) 86
AspLEI GCGC 1 cut(s) 76
AsuII TTCGAA 1 cut(s) 102
BaeGI GKGCMC 1 cut(s) 42
Bbv12I GWGCWC 2 cut(s) 42, 183
BbvI GCAGC 1 cut(s) 73
BceAI ACGGC 1 cut(s) 205
BciT130I CCWGG 1 cut(s) 24
BcoDI GTCTC 2 cut(s) 62, 83
BisI GCNGC 2 cut(s) 87, 123
BlsI GCNGC 2 cut(s) 88, 124
Bme1390I CCNGG 1 cut(s) 24
BmrFI CCNGG 1 cut(s) 24
BmsI GCATC 2 cut(s) 61, 102
Bpu14I TTCGAA 1 cut(s) 102
BsaHI GRCGYC 1 cut(s) 153
BsaJI CCNNGG 1 cut(s) 66
Bsc4I CCNNNNNNNGG 1 cut(s) 67
BseBI CCWGG 1 cut(s) 24
BseDI CCNNGG 1 cut(s) 66
BseGI GGATG 2 cut(s) 8, 76
BseLI CCNNNNNNNGG 1 cut(s) 67
BseMII CTCAG 1 cut(s) 96
BseSI GKGCMC 1 cut(s) 42
BseXI GCAGC 1 cut(s) 73
Bsh1236I CGCG 1 cut(s) 76
BshFI GGCC 1 cut(s) 125
BsiHKAI GWGCWC 2 cut(s) 42, 183
BslI CCNNNNNNNGG 1 cut(s) 67
BsmAI GTCTC 2 cut(s) 62, 83
BsmBI CGTCTC 2 cut(s) 62, 83
BsnI GGCC 1 cut(s) 125
Bsp119I TTCGAA 1 cut(s) 102
Bsp1286I GDGCHC 2 cut(s) 42, 183
Bsp143I GATC 1 cut(s) 189
Bsp19I CCATGG 1 cut(s) 66
BspACI CCGC 2 cut(s) 122, 218
BspANI GGCC 1 cut(s) 125
BspCNI CTCAG 1 cut(s) 95
BspFNI CGCG 1 cut(s) 76
BspPI GGATC 1 cut(s) 184
BspT104I TTCGAA 1 cut(s) 102
BssECI CCNNGG 1 cut(s) 66
BssMI GATC 1 cut(s) 189
BssNI GRCGYC 1 cut(s) 153
BssT1I CCWWGG 1 cut(s) 66
Bst2UI CCWGG 1 cut(s) 24
Bst4CI ACNGT 1 cut(s) 197
BstACI GRCGYC 1 cut(s) 153
BstBI TTCGAA 1 cut(s) 102
BstC8I GCNNGC 2 cut(s) 183, 208
BstDEI CTNAG 1 cut(s) 82
BstDSI CCRYGG 1 cut(s) 66
BstF5I GGATG 2 cut(s) 8, 76
BstFNI CGCG 1 cut(s) 76
BstHHI GCGC 1 cut(s) 76
BstKTI GATC 1 cut(s) 192
BstMAI GTCTC 2 cut(s) 62, 83
BstMBI GATC 1 cut(s) 189
BstMWI GCNNNNNNNGC 2 cut(s) 71, 131
BstNI CCWGG 1 cut(s) 24
BstSCI CCNGG 1 cut(s) 22
BstSLI GKGCMC 1 cut(s) 42
BstUI CGCG 1 cut(s) 76
BstV1I GCAGC 1 cut(s) 73
BstX2I RGATCY 1 cut(s) 189
BstYI RGATCY 1 cut(s) 189
BsuRI GGCC 1 cut(s) 125
BtgI CCRYGG 1 cut(s) 66
BtsCI GGATG 2 cut(s) 8, 76
BtsIMutI CAGTG 1 cut(s) 193
Cac8I GCNNGC 2 cut(s) 183, 208
CfoI GCGC 1 cut(s) 76
CseI GACGC 2 cut(s) 65, 161
CviAII CATG 1 cut(s) 67
CviJI RGCY 4 cut(s) 86, 125, 134, 167
CviKI_1 RGCY 4 cut(s) 86, 125, 134, 167
DdeI CTNAG 1 cut(s) 82
DpnI GATC 1 cut(s) 191
DpnII GATC 1 cut(s) 189
EciI GGCGGA 1 cut(s) 207
Eco130I CCWWGG 1 cut(s) 66
EcoRII CCWGG 1 cut(s) 22
EcoT14I CCWWGG 1 cut(s) 66
ErhI CCWWGG 1 cut(s) 66
Esp3I CGTCTC 2 cut(s) 62, 83
FaeI CATG 1 cut(s) 70
FaiI YATR 4 cut(s) 68, 137, 148, 170
FatI CATG 1 cut(s) 66
Fnu4HI GCNGC 2 cut(s) 87, 123
FokI GGATG 1 cut(s) 83
Fsp4HI GCNGC 2 cut(s) 87, 123
GlaI GCGC 1 cut(s) 75
GluI GCNGC 2 cut(s) 87, 123
HaeIII GGCC 1 cut(s) 125
HgaI GACGC 2 cut(s) 65, 161
HhaI GCGC 1 cut(s) 76
Hin1I GRCGYC 1 cut(s) 153
Hin1II CATG 1 cut(s) 70
Hin6I GCGC 1 cut(s) 74
HinP1I GCGC 1 cut(s) 74
HinfI GANTC 4 cut(s) 19, 95, 140, 172
Hpy166II GTNNAC 1 cut(s) 40
Hpy188III TCNNGA 1 cut(s) 16
Hpy8I GTNNAC 1 cut(s) 40
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 2 cut(s) 22, 109
HpyCH4III ACNGT 1 cut(s) 197
HpyCH4V TGCA 2 cut(s) 40, 89
HpyF10VI GCNNNNNNNGC 2 cut(s) 71, 131
HpyF3I CTNAG 1 cut(s) 82
Hsp92I GRCGYC 1 cut(s) 153
Hsp92II CATG 1 cut(s) 70
HspAI GCGC 1 cut(s) 74
Kzo9I GATC 1 cut(s) 189
LpnPI CCDG 4 cut(s) 9, 36, 139, 189
Lsp1109I GCAGC 1 cut(s) 73
LweI GCATC 2 cut(s) 61, 102
MalI GATC 1 cut(s) 191
MboI GATC 1 cut(s) 189
MfeI CAATTG 1 cut(s) 90
MflI RGATCY 1 cut(s) 189
MhlI GDGCHC 2 cut(s) 42, 183
MluCI AATT 2 cut(s) 90, 201
MlyI GAGTC 1 cut(s) 89
MnlI CCTC 1 cut(s) 42
MseI TTAA 1 cut(s) 213
MspA1I CMGCKG 1 cut(s) 86
MspR9I CCNGG 1 cut(s) 24
MunI CAATTG 1 cut(s) 90
MvaI CCWGG 1 cut(s) 24
MvnI CGCG 1 cut(s) 76
MwoI GCNNNNNNNGC 2 cut(s) 71, 131
NcoI CCATGG 1 cut(s) 66
NdeII GATC 1 cut(s) 189
NlaIII CATG 1 cut(s) 70
NspV TTCGAA 1 cut(s) 102
PfeI GAWTC 3 cut(s) 19, 140, 172
PkrI GCNGC 2 cut(s) 88, 124
PleI GAGTC 1 cut(s) 89
PpsI GAGTC 1 cut(s) 89
Psp6I CCWGG 1 cut(s) 22
PspGI CCWGG 1 cut(s) 22
PsuI RGATCY 1 cut(s) 189
PvuII CAGCTG 1 cut(s) 86
SaqAI TTAA 1 cut(s) 213
SatI GCNGC 2 cut(s) 87, 123
Sau3AI GATC 1 cut(s) 189
SchI GAGTC 1 cut(s) 89
ScrFI CCNGG 1 cut(s) 24
SduI GDGCHC 2 cut(s) 42, 183
SetI ASST 3 cut(s) 88, 136, 169
SfaNI GCATC 2 cut(s) 61, 102
SfuI TTCGAA 1 cut(s) 102
Sse9I AATT 2 cut(s) 90, 201
SsiI CCGC 2 cut(s) 122, 218
StyD4I CCNGG 1 cut(s) 22
StyI CCWWGG 1 cut(s) 66
TaaI ACNGT 1 cut(s) 197
TaqI TCGA 3 cut(s) 17, 102, 110
TasI AATT 2 cut(s) 90, 201
TauI GCSGC 1 cut(s) 125
TfiI GAWTC 3 cut(s) 19, 140, 172
Tru1I TTAA 1 cut(s) 213
Tru9I TTAA 1 cut(s) 213
TscAI CASTG 1 cut(s) 200
TseI GCWGC 1 cut(s) 86
TspDTI ATGAA 1 cut(s) 185
TspRI CASTG 1 cut(s) 200
VneI GTGCAC 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.