pycom01g06530

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0008632
Physical Location & Seq
Forward (+)
6903188 .. 6908691
5504 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1869 bp
ATGTTAGAAGTTTGCCTTTTTCTGTCAAGGAAAACAAAAAAGCCGGCTTTCGCAAACGTTGAAAACGCCGAAAAGCAGAGAACGCCAAAAAAGAAAGAGAAAGGCTTTGACAGCTTGGACTTCAAAACCTCCTGCAGCCTGCTAATAAGTCAGCTCGTACGGCCCTCCGTCGTTGCTTTCTATTTTCCCTCCTTAATTATTTATTTACTCTCTCTCCTCTCTTTCTGCAATTCTTCCAACTCTCCCATTGATCAATACAAACTTGAAGAAATCTTAAGTATTATGGCCACTCTCTCGCACGCGATCGCCCCGCCGGGACTCGGCCCCCACCGCCGCGCTTCTCACGGATTCTCTCCGCCGTCTCTGAGACTACTGACTACTTCCAGTGGAGTCGCCAAGAACAGCAGCCGCGTCTTCGCTCAGAGCAAGAGCCATGGCCGGCAGCAGATCGATTTCAGTGATCCCGACTGGAAATCCAAGTTCCAGAAGGATTTCGAGAAGCGGTTTAACATCCCTCACATCACTGATGTCTTCCCTGACGCCGTTCCCATTCCTTCCACCTTCTGCTTACAAATGAGGACTCCGGTGATTGAAGACTTTGCAGGTGGGTACCCGTCGGACGAAGAGTGGCACGGCTACATAAATAACAATGACCGGGTACTCCTTAAGACCATATACTACTCGTCGCATACGTCTGCTGGTGCTGAATGCATTGATCCTAATTGTACTTGGGTGGAGCAATGGGTTCATCGTGCTGGGCCTCGGGAAAAAATATATTTTAAACCAGAAGAAGTGAAAGCAGCAATTGTAACATGCGGAGGGCTCTGCCCTGGTCTTAATGACGTCATCAGACAGATCGTCATTACACTTGAAATATACGGTGTCAAACAGATTGTGGGGATTCCTTATGGTTACCGCGGATTTTCTGAAAATGAATTAGCTGAACTGCCACTATCTAGGAAAGTGGTTCAAAATGTTCATCTTTCTGGTGGAAGCTTGTTAGGAGTTTCACGTGGAGGTCCCAGCGTTAGTGACATTGTGGACAGTTTGGAGGAAAGAGGGATCAACATGGTTTTTATTTTGGGCGGAAACGGCACACATGCTGGAGCCGATGCAATACACAATGAGTGCCGTAAAAGACAGTTAAGAGTGGCTGTAGTTGGTGTGCCGAAAACCATAGACAATGATATTTTGCTGATGGACAAAACTTTTGGTTTCGACACAGCTGTTGAAGAAGCTCAACGGGCAATAAATTCAGCATACATTGAGGCACATAGTGCTTACCATGGCGTTGGCATTGTTAAATTGATGGGTCGTAGCAGTGGATTCATAGCAATGCATGCATCCCTAGCTAGTGGACAAATTGACATATGTTTGATACCTGAGGTACCTTTTCAAATACATGGCCCTCATGGTATTTTGCACCATCTAAAATACCTAATTCAAACAAAGGGATCAGCTGTGGTCTGCGTAGCAGAGGGAGCAGGGCAGAATTTACTAGAGAAAACCAATGCTACTGATGCATCTGGAAATATGGTATTTGGGGATATTGGCGTACATATTCAGCAAGAGACAAAAAAGTATTTTAAAGAGGCTGGTGTTCCCGTTGACGTGAAGTATATAGACCCCACATACATGATCCGTGCATGTCGTGCAAATGCATCAGACGGAATACTTTGCGCTGTATTAGGGCAAAATGCTGTTCACGGGGCGTTCGCTGGATACAGTGGAATCACAGTTGGCACTTGCAACACTCATTATGTATACCTTCCAATTCCTGAGGTCATATCTCATCCCAGGCTGGTGGATCCGAACAGCCGAATGTGGCATCGTTGCTTGACTTCCACAGGTCAGCCGGACTTTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

623

Amino Acids

68.26

Weight (kDa)

7.6

Isoelectric Point (pI)

38.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 267 - 569 9.3e-61 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 593
AatII GACGTC 1 cut(s) 846
Acc36I ACCTGC 1 cut(s) 593
Acc65I GGTACC 2 cut(s) 609, 1387
AccB1I GGYRCC 2 cut(s) 609, 1387
AccI GTMKAC 1 cut(s) 1764
AccII CGCG 4 cut(s) 302, 336, 411, 918
AclI AACGTT 1 cut(s) 57
AclWI GGATC 7 cut(s) 455, 710, 1070, 1462, 1633, 1802, 1815
AcoI YGGCCR 2 cut(s) 285, 436
AcsI RAATTY 2 cut(s) 1252, 1492
AcvI CACGTG 1 cut(s) 1013
AcyI GRCGYC 2 cut(s) 540, 843
AdeI CACNNNGTG 1 cut(s) 1277
AfaI GTAC 6 cut(s) 159, 611, 660, 727, 1389, 1557
AfiI CCNNNNNNNGG 1 cut(s) 320
AflII CTTAAG 2 cut(s) 274, 665
AgsI TTSAA 9 cut(s) 62, 124, 266, 593, 872, 971, 1232, 1397, 1445
AhdI GACNNNNNGTC 1 cut(s) 857
AjiI CACGTC 1 cut(s) 1612
AjnI CCWGG 2 cut(s) 829, 1796
AluBI AGCT 8 cut(s) 114, 154, 941, 996, 1226, 1238, 1352, 1460
AluI AGCT 8 cut(s) 114, 154, 941, 996, 1226, 1238, 1352, 1460
Alw26I GTCTC 3 cut(s) 361, 366, 1565
AlwI GGATC 7 cut(s) 455, 710, 1070, 1462, 1633, 1802, 1815
Ama87I CYCGRG 1 cut(s) 762
AoxI GGCC 6 cut(s) 161, 285, 322, 436, 758, 1405
ApeKI GCWGC 4 cut(s) 135, 405, 442, 800
ApoI RAATTY 2 cut(s) 1252, 1492
ArsI GACNNNNNNTTYG 2 cut(s) 1193, 1225
AsiSI GCGATCGC 1 cut(s) 306
Asp700I GAANNNNTTC 1 cut(s) 491
Asp718I GGTACC 2 cut(s) 609, 1387
AspLEI GCGC 2 cut(s) 338, 1682
AspS9I GGNCC 5 cut(s) 162, 323, 758, 1019, 1406
AsuC2I CCSGG 2 cut(s) 315, 656
AsuHPI GGTGA 1 cut(s) 598
AvaI CYCGRG 1 cut(s) 762
AvaII GGWCC 1 cut(s) 1019
AxyI CCTNAGG 2 cut(s) 1383, 1779
BalI TGGCCA 1 cut(s) 287
BamHI GGATCC 1 cut(s) 1807
BanI GGYRCC 2 cut(s) 609, 1387
BanII GRGCYC 1 cut(s) 825
BbrPI CACGTG 1 cut(s) 1013
BbsI GAAGAC 3 cut(s) 406, 523, 600
BbvI GCAGC 4 cut(s) 147, 417, 454, 812
BccI CCATC 3 cut(s) 1192, 1303, 1434
BceAI ACGGC 6 cut(s) 176, 343, 527, 649, 1108, 1116
BciT130I CCWGG 2 cut(s) 831, 1798
BciVI GTATCC 1 cut(s) 1715
BclI TGATCA 1 cut(s) 250
BcnI CCSGG 2 cut(s) 315, 656
BcoDI GTCTC 3 cut(s) 361, 366, 1565
BfaI CTAG 5 cut(s) 957, 1349, 1353, 1499, 1867
BfmI CTRYAG 2 cut(s) 133, 1155
BfrI CTTAAG 2 cut(s) 274, 665
BfuAI ACCTGC 1 cut(s) 593
BfuI GTATCC 1 cut(s) 1715
BisI GCNGC 6 cut(s) 136, 334, 406, 409, 443, 801
BlsI GCNGC 6 cut(s) 137, 335, 407, 410, 444, 802
Bme1390I CCNGG 4 cut(s) 315, 656, 831, 1798
Bme18I GGWCC 1 cut(s) 1019
BmeRI GACNNNNNGTC 1 cut(s) 857
BmeT110I CYCGRG 1 cut(s) 762
BmgBI CACGTC 1 cut(s) 1612
BmgT120I GGNCC 5 cut(s) 162, 323, 758, 1019, 1406
BmiI GGNNCC 6 cut(s) 325, 611, 1021, 1108, 1389, 1809
BmrFI CCNGG 4 cut(s) 315, 656, 831, 1798
BmsI GCATC 6 cut(s) 1102, 1352, 1510, 1532, 1670, 1837
BpiI GAAGAC 3 cut(s) 406, 523, 600
BpmI CTGGAG 1 cut(s) 1125
BpuMI CCSGG 2 cut(s) 315, 656
Bsa29I ATCGAT 1 cut(s) 450
BsaAI YACGTR 1 cut(s) 1013
BsaHI GRCGYC 2 cut(s) 540, 843
BsaJI CCNNGG 6 cut(s) 433, 761, 829, 916, 1285, 1796
BsaWI WCCGGW 1 cut(s) 583
BsaXI ACNNNNNCTCC 2 cut(s) 198, 228
Bsc4I CCNNNNNNNGG 1 cut(s) 320
Bse118I RCCGGY 2 cut(s) 43, 438
Bse1I ACTGG 2 cut(s) 384, 473
Bse21I CCTNAGG 2 cut(s) 1383, 1779
Bse3DI GCAATG 2 cut(s) 746, 1341
BseBI CCWGG 2 cut(s) 831, 1798
BseCI ATCGAT 1 cut(s) 450
BseDI CCNNGG 6 cut(s) 433, 761, 829, 916, 1285, 1796
BseGI GGATG 3 cut(s) 510, 1343, 1792
BseLI CCNNNNNNNGG 1 cut(s) 320
BseMI GCAATG 2 cut(s) 746, 1341
BseMII CTCAG 4 cut(s) 356, 434, 1374, 1770
BseNI ACTGG 2 cut(s) 384, 473
BseRI GAGGAG 1 cut(s) 206
BseXI GCAGC 4 cut(s) 147, 417, 454, 812
BseYI CCCAGC 2 cut(s) 755, 1022
Bsh1236I CGCG 4 cut(s) 302, 336, 411, 918
Bsh1285I CGRYCG 1 cut(s) 306
BshFI GGCC 6 cut(s) 163, 287, 324, 438, 760, 1407
BshNI GGYRCC 2 cut(s) 609, 1387
BshVI ATCGAT 1 cut(s) 450
BsiEI CGRYCG 1 cut(s) 306
BsiHKCI CYCGRG 1 cut(s) 762
BsiSI CCGG 6 cut(s) 44, 314, 439, 584, 655, 1856
BsiWI CGTACG 1 cut(s) 157
BslFI GGGAC 2 cut(s) 330, 1005
BslI CCNNNNNNNGG 1 cut(s) 320
BsmAI GTCTC 3 cut(s) 361, 366, 1565
BsmBI CGTCTC 1 cut(s) 366
BsmFI GGGAC 2 cut(s) 330, 1005
BsmI GAATGC 1 cut(s) 713
BsnI GGCC 6 cut(s) 163, 287, 324, 438, 760, 1407
BsoBI CYCGRG 1 cut(s) 762
Bsp1286I GDGCHC 1 cut(s) 825
Bsp19I CCATGG 2 cut(s) 433, 1285
BspANI GGCC 6 cut(s) 163, 287, 324, 438, 760, 1407
BspCNI CTCAG 4 cut(s) 357, 433, 1375, 1771
BspDI ATCGAT 1 cut(s) 450
BspFNI CGCG 4 cut(s) 302, 336, 411, 918
BspLI GGNNCC 6 cut(s) 325, 611, 1021, 1108, 1389, 1809
BspMAI CTGCAG 1 cut(s) 137
BspMI ACCTGC 1 cut(s) 593
BspPI GGATC 7 cut(s) 455, 710, 1070, 1462, 1633, 1802, 1815
BspT107I GGYRCC 2 cut(s) 609, 1387
BspTI CTTAAG 2 cut(s) 274, 665
BsrDI GCAATG 2 cut(s) 746, 1341
BsrFI RCCGGY 2 cut(s) 43, 438
BsrI ACTGG 2 cut(s) 384, 473
BssAI RCCGGY 2 cut(s) 43, 438
BssECI CCNNGG 6 cut(s) 433, 761, 829, 916, 1285, 1796
BssNAI GTATAC 1 cut(s) 1765
BssNI GRCGYC 2 cut(s) 540, 843
BssT1I CCWWGG 2 cut(s) 433, 1285
Bst1107I GTATAC 1 cut(s) 1765
Bst2UI CCWGG 2 cut(s) 831, 1798
Bst4CI ACNGT 5 cut(s) 881, 1046, 1143, 1727, 1738
Bst6I CTCTTC 1 cut(s) 618
BstACI GRCGYC 2 cut(s) 540, 843
BstAFI CTTAAG 2 cut(s) 274, 665
BstAPI GCANNNNNTGC 3 cut(s) 1277, 1340, 1652
BstBAI YACGTR 1 cut(s) 1013
BstC8I GCNNGC 5 cut(s) 45, 140, 300, 440, 1341
BstDEI CTNAG 4 cut(s) 365, 420, 1383, 1779
BstDSI CCRYGG 3 cut(s) 433, 916, 1285
BstEII GGTNACC 1 cut(s) 911
BstF5I GGATG 3 cut(s) 510, 1343, 1792
BstFNI CGCG 4 cut(s) 302, 336, 411, 918
BstHHI GCGC 2 cut(s) 338, 1682
BstMAI GTCTC 3 cut(s) 361, 366, 1565
BstMCI CGRYCG 1 cut(s) 306
BstNI CCWGG 2 cut(s) 831, 1798
BstNSI RCATGY 4 cut(s) 816, 1103, 1343, 1650
BstPI GGTNACC 1 cut(s) 911
BstSCI CCNGG 4 cut(s) 313, 654, 829, 1796
BstSFI CTRYAG 2 cut(s) 133, 1155
BstUI CGCG 4 cut(s) 302, 336, 411, 918
BstV1I GCAGC 4 cut(s) 147, 417, 454, 812
BstV2I GAAGAC 3 cut(s) 406, 523, 600
BstX2I RGATCY 1 cut(s) 1807
BstXI CCANNNNNNTGG 2 cut(s) 1292, 1804
BstYI RGATCY 1 cut(s) 1807
BstZ17I GTATAC 1 cut(s) 1765
Bsu15I ATCGAT 1 cut(s) 450
Bsu36I CCTNAGG 2 cut(s) 1383, 1779
BsuI GTATCC 1 cut(s) 1715
BsuRI GGCC 6 cut(s) 163, 287, 324, 438, 760, 1407
BsuTUI ATCGAT 1 cut(s) 450
BtgI CCRYGG 3 cut(s) 433, 916, 1285
BtrI CACGTC 1 cut(s) 1612
BtsCI GGATG 3 cut(s) 510, 1343, 1792
BtsI GCAGTG 1 cut(s) 1327
BtsIMutI CAGTG 5 cut(s) 391, 463, 522, 1327, 1732
BveI ACCTGC 1 cut(s) 593
Cac8I GCNNGC 5 cut(s) 45, 140, 300, 440, 1341
CfoI GCGC 2 cut(s) 338, 1682
Cfr10I RCCGGY 2 cut(s) 43, 438
Cfr13I GGNCC 5 cut(s) 162, 323, 758, 1019, 1406
Cfr42I CCGCGG 1 cut(s) 919
ClaI ATCGAT 1 cut(s) 450
CseI GACGC 2 cut(s) 400, 548
Csp6I GTAC 6 cut(s) 158, 610, 659, 726, 1388, 1556
CviQI GTAC 6 cut(s) 158, 610, 659, 726, 1388, 1556
DdeI CTNAG 4 cut(s) 365, 420, 1383, 1779
DraI TTTAAA 2 cut(s) 781, 1588
DraIII CACNNNGTG 1 cut(s) 1277
DriI GACNNNNNGTC 1 cut(s) 857
EaeI YGGCCR 2 cut(s) 285, 436
Eam1104I CTCTTC 1 cut(s) 618
Eam1105I GACNNNNNGTC 1 cut(s) 857
EarI CTCTTC 1 cut(s) 618
EciI GGCGGA 2 cut(s) 345, 1101
Eco130I CCWWGG 2 cut(s) 433, 1285
Eco24I GRGCYC 1 cut(s) 825
Eco47I GGWCC 1 cut(s) 1019
Eco72I CACGTG 1 cut(s) 1013
Eco81I CCTNAGG 2 cut(s) 1383, 1779
Eco88I CYCGRG 1 cut(s) 762
Eco91I GGTNACC 1 cut(s) 911
EcoO109I RGGNCCY 1 cut(s) 1019
EcoO65I GGTNACC 1 cut(s) 911
EcoRII CCWGG 2 cut(s) 829, 1796
EcoT14I CCWWGG 2 cut(s) 433, 1285
EcoT22I ATGCAT 5 cut(s) 713, 1341, 1345, 1525, 1663
EcoT38I GRGCYC 1 cut(s) 825
ErhI CCWWGG 2 cut(s) 433, 1285
Esp3I CGTCTC 1 cut(s) 366
FalI AAGNNNNNCTT 1 cut(s) 32
FaqI GGGAC 2 cut(s) 330, 1005
FauI CCCGC 1 cut(s) 318
FauNDI CATATG 1 cut(s) 1370
FbaI TGATCA 1 cut(s) 250
FblI GTMKAC 1 cut(s) 1764
Fnu4HI GCNGC 6 cut(s) 136, 334, 406, 409, 443, 801
FokI GGATG 3 cut(s) 497, 1330, 1779
FriOI GRGCYC 1 cut(s) 825
Fsp4HI GCNGC 6 cut(s) 136, 334, 406, 409, 443, 801
FspBI CTAG 5 cut(s) 957, 1349, 1353, 1499, 1867
GlaI GCGC 2 cut(s) 337, 1681
GluI GCNGC 6 cut(s) 136, 334, 406, 409, 443, 801
GsaI CCCAGC 2 cut(s) 759, 1026
GsuI CTGGAG 1 cut(s) 1125
HaeIII GGCC 6 cut(s) 163, 287, 324, 438, 760, 1407
HapII CCGG 6 cut(s) 44, 314, 439, 584, 655, 1856
HgaI GACGC 2 cut(s) 400, 548
HhaI GCGC 2 cut(s) 338, 1682
Hin1I GRCGYC 2 cut(s) 540, 843
Hin6I GCGC 2 cut(s) 336, 1680
HinP1I GCGC 2 cut(s) 336, 1680
HincII GTYRAC 1 cut(s) 1609
HindII GTYRAC 1 cut(s) 1609
HindIII AAGCTT 1 cut(s) 994
HinfI GANTC 7 cut(s) 318, 348, 390, 580, 901, 1326, 1731
HpaII CCGG 6 cut(s) 44, 314, 439, 584, 655, 1856
HphI GGTGA 1 cut(s) 598
Hpy166II GTNNAC 5 cut(s) 1042, 1358, 1609, 1705, 1765
Hpy188I TCNGA 7 cut(s) 366, 423, 619, 851, 928, 1666, 1812
Hpy188III TCNNGA 6 cut(s) 464, 484, 496, 764, 1527, 1778
Hpy8I GTNNAC 5 cut(s) 1042, 1358, 1609, 1705, 1765
Hpy99I CGWCG 3 cut(s) 173, 619, 688
HpyAV CCTTC 4 cut(s) 481, 564, 571, 1778
HpyCH4III ACNGT 5 cut(s) 881, 1046, 1143, 1727, 1738
HpyCH4IV ACGT 5 cut(s) 57, 692, 843, 1012, 1611
HpyF3I CTNAG 4 cut(s) 365, 420, 1383, 1779
HpySE526I ACGT 5 cut(s) 57, 692, 843, 1012, 1611
Hsp92I GRCGYC 2 cut(s) 540, 843
HspAI GCGC 2 cut(s) 336, 1680
KpnI GGTACC 2 cut(s) 613, 1391
KroI GCCGGC 2 cut(s) 43, 438
KroNI GCCGGC 2 cut(s) 45, 440
Ksp22I TGATCA 1 cut(s) 250
KspI CCGCGG 1 cut(s) 919
LmnI GCTCC 3 cut(s) 736, 1106, 1481
Lsp1109I GCAGC 4 cut(s) 147, 417, 454, 812
LweI GCATC 6 cut(s) 1102, 1352, 1510, 1532, 1670, 1837
MaeI CTAG 5 cut(s) 957, 1349, 1353, 1499, 1867
MaeII ACGT 5 cut(s) 57, 692, 843, 1012, 1611
MaeIII GTNAC 3 cut(s) 808, 911, 1031
MboII GAAGA 8 cut(s) 225, 278, 406, 523, 605, 635, 800, 1244
MfeI CAATTG 1 cut(s) 804
MflI RGATCY 1 cut(s) 1807
MhlI GDGCHC 1 cut(s) 825
MlsI TGGCCA 1 cut(s) 287
MluNI TGGCCA 1 cut(s) 287
MlyI GAGTC 3 cut(s) 312, 399, 574
MmeI TCCRAC 2 cut(s) 261, 597
Mox20I TGGCCA 1 cut(s) 287
Mph1103I ATGCAT 5 cut(s) 713, 1341, 1345, 1525, 1663
MroNI GCCGGC 2 cut(s) 43, 438
MroXI GAANNNNTTC 1 cut(s) 491
MscI TGGCCA 1 cut(s) 287
MseI TTAA 9 cut(s) 194, 275, 507, 666, 780, 837, 1145, 1302, 1587
MslI CAYNNNNRTG 2 cut(s) 1334, 1634
Msp20I TGGCCA 1 cut(s) 287
MspA1I CMGCKG 3 cut(s) 918, 1226, 1460
MspCI CTTAAG 2 cut(s) 274, 665
MspI CCGG 6 cut(s) 44, 314, 439, 584, 655, 1856
MspR9I CCNGG 4 cut(s) 315, 656, 831, 1798
MunI CAATTG 1 cut(s) 804
Mva1269I GAATGC 1 cut(s) 713
MvaI CCWGG 2 cut(s) 831, 1798
MvnI CGCG 4 cut(s) 302, 336, 411, 918
NaeI GCCGGC 2 cut(s) 45, 440
NciI CCSGG 2 cut(s) 315, 656
NcoI CCATGG 2 cut(s) 433, 1285
NdeI CATATG 1 cut(s) 1370
NgoMIV GCCGGC 2 cut(s) 43, 438
NlaIV GGNNCC 6 cut(s) 325, 611, 1021, 1108, 1389, 1809
NmeAIII GCCGAG 1 cut(s) 300
NmuCI GTSAC 1 cut(s) 1031
NsiI ATGCAT 5 cut(s) 713, 1341, 1345, 1525, 1663
NspI RCATGY 4 cut(s) 816, 1103, 1343, 1650
PaeI GCATGC 1 cut(s) 1343
PaqCI CACCTGC 1 cut(s) 593
PcsI WCGNNNNNNNCGW 1 cut(s) 689
PctI GAATGC 1 cut(s) 713
PdiI GCCGGC 2 cut(s) 45, 440
PdmI GAANNNNTTC 1 cut(s) 491
PfeI GAWTC 4 cut(s) 348, 901, 1326, 1731
Pfl23II CGTACG 1 cut(s) 157
PkrI GCNGC 6 cut(s) 137, 335, 407, 410, 444, 802
Ple19I CGATCG 1 cut(s) 306
PleI GAGTC 3 cut(s) 312, 398, 574
PmaCI CACGTG 1 cut(s) 1013
PmlI CACGTG 1 cut(s) 1013
PpsI GAGTC 3 cut(s) 312, 398, 574
Ppu21I YACGTR 1 cut(s) 1013
PpuMI RGGWCCY 1 cut(s) 1019
Psp1406I AACGTT 1 cut(s) 57
Psp5II RGGWCCY 1 cut(s) 1019
Psp6I CCWGG 2 cut(s) 829, 1796
PspCI CACGTG 1 cut(s) 1013
PspEI GGTNACC 1 cut(s) 911
PspFI CCCAGC 2 cut(s) 755, 1022
PspGI CCWGG 2 cut(s) 829, 1796
PspLI CGTACG 1 cut(s) 157
PspN4I GGNNCC 6 cut(s) 325, 611, 1021, 1108, 1389, 1809
PspPI GGNCC 5 cut(s) 162, 323, 758, 1019, 1406
PspPPI RGGWCCY 1 cut(s) 1019
PstI CTGCAG 1 cut(s) 137
PsuI RGATCY 1 cut(s) 1807
PvuI CGATCG 1 cut(s) 306
PvuII CAGCTG 2 cut(s) 1226, 1460
RgaI GCGATCGC 1 cut(s) 306
RsaI GTAC 6 cut(s) 159, 611, 660, 727, 1389, 1557
RsaNI GTAC 6 cut(s) 158, 610, 659, 726, 1388, 1556
RseI CAYNNNNRTG 2 cut(s) 1334, 1634
SacII CCGCGG 1 cut(s) 919
SaqAI TTAA 9 cut(s) 194, 275, 507, 666, 780, 837, 1145, 1302, 1587
SatI GCNGC 6 cut(s) 136, 334, 406, 409, 443, 801
Sau96I GGNCC 5 cut(s) 162, 323, 758, 1019, 1406
SchI GAGTC 3 cut(s) 312, 399, 574
ScrFI CCNGG 4 cut(s) 315, 656, 831, 1798
SduI GDGCHC 1 cut(s) 825
SfaAI GCGATCGC 1 cut(s) 306
SfaNI GCATC 6 cut(s) 1102, 1352, 1510, 1532, 1670, 1837
SfcI CTRYAG 2 cut(s) 133, 1155
Sfr303I CCGCGG 1 cut(s) 919
SgfI GCGATCGC 1 cut(s) 306
SgrBI CCGCGG 1 cut(s) 919
SinI GGWCC 1 cut(s) 1019
SmiMI CAYNNNNRTG 2 cut(s) 1334, 1634
SmlI CTYRAG 2 cut(s) 274, 665
SmoI CTYRAG 2 cut(s) 274, 665
SphI GCATGC 1 cut(s) 1343
SspMI CTAG 5 cut(s) 957, 1349, 1353, 1499, 1867
StyD4I CCNGG 4 cut(s) 313, 654, 829, 1796
StyI CCWWGG 2 cut(s) 433, 1285
TaaI ACNGT 5 cut(s) 881, 1046, 1143, 1727, 1738
TaiI ACGT 5 cut(s) 60, 695, 846, 1015, 1614
TaqI TCGA 3 cut(s) 450, 495, 1218
TatI WGTACW 1 cut(s) 725
TauI GCSGC 2 cut(s) 336, 411
TfiI GAWTC 4 cut(s) 348, 901, 1326, 1731
Tru1I TTAA 9 cut(s) 194, 275, 507, 666, 780, 837, 1145, 1302, 1587
Tru9I TTAA 9 cut(s) 194, 275, 507, 666, 780, 837, 1145, 1302, 1587
TscAI CASTG 5 cut(s) 391, 463, 529, 1327, 1732
TseFI GTSAC 1 cut(s) 1031
TseI GCWGC 4 cut(s) 135, 405, 442, 800
Tsp45I GTSAC 1 cut(s) 1031
TspDTI ATGAA 4 cut(s) 737, 948, 968, 1318
TspGWI ACGGA 4 cut(s) 157, 360, 1631, 1683
TspRI CASTG 5 cut(s) 391, 463, 529, 1327, 1732
Vha464I CTTAAG 2 cut(s) 274, 665
VpaK11BI GGWCC 1 cut(s) 1019
XapI RAATTY 2 cut(s) 1252, 1492
XceI RCATGY 4 cut(s) 816, 1103, 1343, 1650
XmiI GTMKAC 1 cut(s) 1764
XmnI GAANNNNTTC 1 cut(s) 491
XspI CTAG 5 cut(s) 957, 1349, 1353, 1499, 1867
ZraI GACGTC 1 cut(s) 844
Zsp2I ATGCAT 5 cut(s) 713, 1341, 1345, 1525, 1663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.