pycom01g06560

14-3-3-like protein GF14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0004574
Physical Location & Seq
Reverse (-)
6967712 .. 6969134
1423 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1095 bp
ATGTTGGATAGGAACCAAACGAAAGCAACAGAAACCTGTAACGAAAGGCCGTTGGATAGGAACCAAACGAAAGCAACAGAAACCTGTAACGAAAGGCCATTGGGCTTTTTGTGGATTGAAAATACAACGACCTGGCCTCCCGGCCCAAGCCAAGCCCCAGCAGTAAACGTACCTTTTTCGACCAAAGTTGTTCAAAATCAAACGAATCTCAACCCCTCTCGCTCTCCGGTCGCACTCACCTCTTCCTCCTCTCTCCAACTCTATCACTCCTCAGCTCCTCCAATGGCTTCCGCCGTCCCAGAAAACCTCAGCCGCGAGCAGTACGTGTACCTGGCGAAGCTCGCCGAGCAGGCCGAGCGCTACGAGGAGATGGTGAGCTTCATGGAGAAGCTCGTGGTGAGCTCCACCGCCGCCGGGACCGAGCTGACGGTGGAAGAGCGCAATCTGCTCAGCGTCGCCTACAAGAATGTCATCGGCTCGCTCCGCGCGGCCTGGCGCATAATCTCGTCGATCGAGCAGAAGGAGGAAAGCCGTCGCAACGAGGAGCACGTGGCGCTCGTCAAGCAGTACAGATCCAAGGTGGAGACTGAGCTCTCCGCCATCTGCGCTGGAATCCTGGAGCTCCTGCAATCGCACCTTGTTCCTTCGGCGACCACAGGAGAGTCCAAGGTCTTCTACTTGAAGATGAAGGGCGATTATCATCGATACCTCGCCGAGTTCAAAAATGGCGACGAGAGGAAGACTGCCGCCGAGGACACCATGGTTGCATACAAGGCTGCTCAGGATATTGCGGCGAGTGATTTGGCGCCCACGCATCCAATAAGGTTGGGGCTGGCGCTCAACTTTTCGGTGTTTTACTACGAGATCCTCAACTCCTCGGAGAAAGCTTGTAGCATGGCTAAACAGGTTTGGACTGGTTGCGAAATTCTTCATTTTATTGATTTAAGAACTTGTAATTCTTTGTTTGTTTTTCATTTTGTAATACTTGATCATATATGGATGAAAGAGGAAACACTGAGGATGTTGAATTCGAGAATGCGCGTGAAAAAAGTCAATTGCTTTTACCTGTCTAGTTATTTGTTTGTTTGGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

365

Amino Acids

41.3

Weight (kDa)

6.73

Isoelectric Point (pI)

53.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 111 - 303 8.7e-82 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 805
AccII CGCG 4 cut(s) 315, 486, 488, 1041
AciI CCGC 9 cut(s) 291, 313, 408, 411, 484, 488, 597, 747, 791
AclWI GGATC 2 cut(s) 567, 859
AcsI RAATTY 2 cut(s) 924, 1027
AcvI CACGTG 1 cut(s) 550
AcyI GRCGYC 1 cut(s) 806
AfaI GTAC 4 cut(s) 171, 323, 329, 569
AfeI AGCGCT 1 cut(s) 359
AfiI CCNNNNNNNGG 1 cut(s) 414
AflIII ACRYGT 1 cut(s) 324
AgsI TTSAA 5 cut(s) 119, 194, 682, 721, 1027
AjnI CCWGG 4 cut(s) 131, 330, 491, 615
AluBI AGCT 9 cut(s) 275, 340, 378, 391, 402, 424, 592, 622, 887
AluI AGCT 9 cut(s) 275, 340, 378, 391, 402, 424, 592, 622, 887
Alw21I GWGCWC 4 cut(s) 404, 549, 594, 624
Alw26I GTCTC 1 cut(s) 578
AlwI GGATC 2 cut(s) 567, 859
Aor51HI AGCGCT 1 cut(s) 359
AoxI GGCC 6 cut(s) 47, 95, 134, 142, 351, 489
ApeKI GCWGC 1 cut(s) 776
ApoI RAATTY 2 cut(s) 924, 1027
Asp700I GAANNNNTTC 1 cut(s) 927
AspLEI GCGC 9 cut(s) 360, 441, 488, 498, 556, 608, 808, 838, 1041
AspS9I GGNCC 2 cut(s) 143, 417
AsuC2I CCSGG 2 cut(s) 141, 415
AsuHPI GGTGA 3 cut(s) 229, 385, 409
AvaII GGWCC 1 cut(s) 417
BanI GGYRCC 1 cut(s) 805
BanII GRGCYC 3 cut(s) 404, 594, 624
BauI CACGAG 1 cut(s) 392
BbrPI CACGTG 1 cut(s) 550
BbsI GAAGAC 2 cut(s) 664, 746
Bbv12I GWGCWC 4 cut(s) 404, 549, 594, 624
BbvCI CCTCAGC 2 cut(s) 271, 308
BbvI GCAGC 1 cut(s) 763
BccI CCATC 2 cut(s) 364, 608
BceAI ACGGC 3 cut(s) 34, 278, 516
BciT130I CCWGG 4 cut(s) 133, 332, 493, 617
BclI TGATCA 1 cut(s) 988
BcnI CCSGG 2 cut(s) 141, 415
BcoDI GTCTC 1 cut(s) 578
BfaI CTAG 1 cut(s) 1071
BfoI RGCGCY 4 cut(s) 361, 557, 809, 839
BglI GCCNNNNNGGC 1 cut(s) 350
BisI GCNGC 6 cut(s) 313, 411, 489, 747, 777, 792
BlpI GCTNAGC 1 cut(s) 449
BlsI GCNGC 6 cut(s) 314, 412, 490, 748, 778, 793
Bme1390I CCNGG 6 cut(s) 133, 141, 332, 415, 493, 617
Bme18I GGWCC 1 cut(s) 417
BmgT120I GGNCC 2 cut(s) 143, 417
BmiI GGNNCC 4 cut(s) 14, 62, 418, 807
BmrFI CCNGG 6 cut(s) 133, 141, 332, 415, 493, 617
BmsI GCATC 1 cut(s) 823
BpiI GAAGAC 2 cut(s) 664, 746
BpmI CTGGAG 1 cut(s) 638
Bpu10I CCTNAGC 3 cut(s) 271, 308, 780
Bpu1102I GCTNAGC 1 cut(s) 449
BpuMI CCSGG 2 cut(s) 141, 415
Bsa29I ATCGAT 1 cut(s) 703
BsaAI YACGTR 2 cut(s) 325, 550
BsaBI GATNNNNATC 1 cut(s) 699
BsaHI GRCGYC 1 cut(s) 806
BsaJI CCNNGG 5 cut(s) 576, 666, 750, 759, 876
BsaWI WCCGGW 1 cut(s) 226
BsaXI ACNNNNNCTCC 2 cut(s) 121, 151
Bsc4I CCNNNNNNNGG 1 cut(s) 414
Bse1I ACTGG 1 cut(s) 919
Bse8I GATNNNNATC 1 cut(s) 699
BseBI CCWGG 4 cut(s) 133, 332, 493, 617
BseCI ATCGAT 1 cut(s) 703
BseDI CCNNGG 5 cut(s) 576, 666, 750, 759, 876
BseGI GGATG 3 cut(s) 814, 1005, 1026
BseJI GATNNNNATC 1 cut(s) 699
BseLI CCNNNNNNNGG 1 cut(s) 414
BseMII CTCAG 6 cut(s) 285, 322, 463, 579, 794, 1007
BseNI ACTGG 1 cut(s) 919
BseRI GAGGAG 6 cut(s) 238, 259, 267, 380, 557, 865
BseXI GCAGC 1 cut(s) 763
BseYI CCCAGC 1 cut(s) 157
Bsh1236I CGCG 4 cut(s) 315, 486, 488, 1041
Bsh1285I CGRYCG 2 cut(s) 231, 513
BshFI GGCC 6 cut(s) 49, 97, 136, 144, 353, 491
BshNI GGYRCC 1 cut(s) 805
BshVI ATCGAT 1 cut(s) 703
BsiEI CGRYCG 2 cut(s) 231, 513
BsiHKAI GWGCWC 4 cut(s) 404, 549, 594, 624
BsiSI CCGG 3 cut(s) 141, 227, 414
BslFI GGGAC 2 cut(s) 281, 430
BslI CCNNNNNNNGG 1 cut(s) 414
BsmAI GTCTC 1 cut(s) 578
BsmFI GGGAC 2 cut(s) 281, 430
BsmI GAATGC 1 cut(s) 1041
BsnI GGCC 6 cut(s) 49, 97, 136, 144, 353, 491
Bsp1286I GDGCHC 4 cut(s) 404, 549, 594, 624
Bsp143I GATC 4 cut(s) 510, 572, 864, 988
Bsp1720I GCTNAGC 1 cut(s) 449
Bsp19I CCATGG 1 cut(s) 759
BspACI CCGC 9 cut(s) 291, 313, 408, 411, 484, 488, 597, 747, 791
BspANI GGCC 6 cut(s) 49, 97, 136, 144, 353, 491
BspCNI CTCAG 6 cut(s) 284, 321, 462, 580, 793, 1008
BspDI ATCGAT 1 cut(s) 703
BspFNI CGCG 4 cut(s) 315, 486, 488, 1041
BspLI GGNNCC 4 cut(s) 14, 62, 418, 807
BspPI GGATC 2 cut(s) 567, 859
BspQI GCTCTTC 1 cut(s) 429
BspT107I GGYRCC 1 cut(s) 805
BsrI ACTGG 1 cut(s) 919
BssECI CCNNGG 5 cut(s) 576, 666, 750, 759, 876
BssMI GATC 4 cut(s) 510, 572, 864, 988
BssNI GRCGYC 1 cut(s) 806
BssSI CACGAG 1 cut(s) 392
BssT1I CCWWGG 3 cut(s) 576, 666, 759
Bst2BI CACGAG 1 cut(s) 392
Bst2UI CCWGG 4 cut(s) 133, 332, 493, 617
Bst4CI ACNGT 1 cut(s) 430
Bst6I CTCTTC 2 cut(s) 247, 429
BstACI GRCGYC 1 cut(s) 806
BstBAI YACGTR 2 cut(s) 325, 550
BstC8I GCNNGC 5 cut(s) 317, 342, 351, 479, 834
BstDEI CTNAG 6 cut(s) 271, 308, 449, 588, 780, 1016
BstDSI CCRYGG 1 cut(s) 759
BstF5I GGATG 3 cut(s) 814, 1005, 1026
BstFNI CGCG 4 cut(s) 315, 486, 488, 1041
BstH2I RGCGCY 4 cut(s) 361, 557, 809, 839
BstHHI GCGC 9 cut(s) 360, 441, 488, 498, 556, 608, 808, 838, 1041
BstKTI GATC 4 cut(s) 513, 575, 867, 991
BstMAI GTCTC 1 cut(s) 578
BstMBI GATC 4 cut(s) 510, 572, 864, 988
BstMCI CGRYCG 2 cut(s) 231, 513
BstNI CCWGG 4 cut(s) 133, 332, 493, 617
BstSCI CCNGG 6 cut(s) 131, 139, 330, 413, 491, 615
BstUI CGCG 4 cut(s) 315, 486, 488, 1041
BstV1I GCAGC 1 cut(s) 763
BstV2I GAAGAC 2 cut(s) 664, 746
BstX2I RGATCY 2 cut(s) 572, 864
BstYI RGATCY 2 cut(s) 572, 864
Bsu15I ATCGAT 1 cut(s) 703
BsuRI GGCC 6 cut(s) 49, 97, 136, 144, 353, 491
BsuTUI ATCGAT 1 cut(s) 703
BtgI CCRYGG 1 cut(s) 759
BtsCI GGATG 3 cut(s) 814, 1005, 1026
BtsIMutI CAGTG 1 cut(s) 1013
Cac8I GCNNGC 5 cut(s) 317, 342, 351, 479, 834
CfoI GCGC 9 cut(s) 360, 441, 488, 498, 556, 608, 808, 838, 1041
Cfr13I GGNCC 2 cut(s) 143, 417
ClaI ATCGAT 1 cut(s) 703
CseI GACGC 1 cut(s) 442
Csp6I GTAC 4 cut(s) 170, 322, 328, 568
CviAII CATG 3 cut(s) 382, 760, 895
CviQI GTAC 4 cut(s) 170, 322, 328, 568
DdeI CTNAG 6 cut(s) 271, 308, 449, 588, 780, 1016
DinI GGCGCC 1 cut(s) 807
DpnI GATC 4 cut(s) 512, 574, 866, 990
DpnII GATC 4 cut(s) 510, 572, 864, 988
Eam1104I CTCTTC 2 cut(s) 247, 429
EarI CTCTTC 2 cut(s) 247, 429
EciI GGCGGA 2 cut(s) 280, 586
Ecl136II GAGCTC 3 cut(s) 402, 592, 622
Eco130I CCWWGG 3 cut(s) 576, 666, 759
Eco24I GRGCYC 3 cut(s) 404, 594, 624
Eco47I GGWCC 1 cut(s) 417
Eco47III AGCGCT 1 cut(s) 359
Eco53kI GAGCTC 3 cut(s) 402, 592, 622
Eco72I CACGTG 1 cut(s) 550
EcoICRI GAGCTC 3 cut(s) 402, 592, 622
EcoRI GAATTC 1 cut(s) 1027
EcoRII CCWGG 4 cut(s) 131, 330, 491, 615
EcoT14I CCWWGG 3 cut(s) 576, 666, 759
EcoT38I GRGCYC 3 cut(s) 404, 594, 624
EgeI GGCGCC 1 cut(s) 807
EheI GGCGCC 1 cut(s) 807
ErhI CCWWGG 3 cut(s) 576, 666, 759
FaeI CATG 3 cut(s) 385, 763, 898
FaiI YATR 8 cut(s) 383, 500, 761, 769, 896, 993, 995, 997
FaqI GGGAC 2 cut(s) 281, 430
FatI CATG 3 cut(s) 381, 759, 894
FbaI TGATCA 1 cut(s) 988
Fnu4HI GCNGC 6 cut(s) 313, 411, 489, 747, 777, 792
FokI GGATG 3 cut(s) 801, 1012, 1033
FriOI GRGCYC 3 cut(s) 404, 594, 624
Fsp4HI GCNGC 6 cut(s) 313, 411, 489, 747, 777, 792
FspBI CTAG 1 cut(s) 1071
GlaI GCGC 9 cut(s) 359, 440, 487, 497, 555, 607, 807, 837, 1040
GluI GCNGC 6 cut(s) 313, 411, 489, 747, 777, 792
GsaI CCCAGC 1 cut(s) 161
GsuI CTGGAG 1 cut(s) 638
HaeII RGCGCY 4 cut(s) 361, 557, 809, 839
HaeIII GGCC 6 cut(s) 49, 97, 136, 144, 353, 491
HapII CCGG 3 cut(s) 141, 227, 414
HgaI GACGC 1 cut(s) 442
HhaI GCGC 9 cut(s) 360, 441, 488, 498, 556, 608, 808, 838, 1041
Hin1I GRCGYC 1 cut(s) 806
Hin1II CATG 3 cut(s) 385, 763, 898
Hin6I GCGC 9 cut(s) 358, 439, 486, 496, 554, 606, 806, 836, 1039
HinP1I GCGC 9 cut(s) 358, 439, 486, 496, 554, 606, 806, 836, 1039
HindIII AAGCTT 1 cut(s) 885
HinfI GANTC 3 cut(s) 205, 612, 662
HpaII CCGG 3 cut(s) 141, 227, 414
HphI GGTGA 3 cut(s) 229, 385, 409
Hpy166II GTNNAC 2 cut(s) 166, 328
Hpy188I TCNGA 1 cut(s) 880
Hpy188III TCNNGA 2 cut(s) 782, 1032
Hpy8I GTNNAC 2 cut(s) 166, 328
Hpy99I CGWCG 4 cut(s) 458, 511, 537, 734
HpyAV CCTTC 3 cut(s) 514, 654, 682
HpyCH4III ACNGT 1 cut(s) 430
HpyCH4IV ACGT 3 cut(s) 168, 324, 549
HpyCH4V TGCA 2 cut(s) 628, 767
HpyF3I CTNAG 6 cut(s) 271, 308, 449, 588, 780, 1016
HpySE526I ACGT 3 cut(s) 168, 324, 549
Hsp92I GRCGYC 1 cut(s) 806
Hsp92II CATG 3 cut(s) 385, 763, 898
HspAI GCGC 9 cut(s) 358, 439, 486, 496, 554, 606, 806, 836, 1039
KasI GGCGCC 1 cut(s) 805
Ksp22I TGATCA 1 cut(s) 988
Kzo9I GATC 4 cut(s) 510, 572, 864, 988
LguI GCTCTTC 1 cut(s) 429
LmnI GCTCC 6 cut(s) 280, 407, 486, 544, 619, 627
Lsp1109I GCAGC 1 cut(s) 763
LweI GCATC 1 cut(s) 823
MaeI CTAG 1 cut(s) 1071
MaeII ACGT 3 cut(s) 168, 324, 549
MaeIII GTNAC 2 cut(s) 38, 86
MalI GATC 4 cut(s) 512, 574, 866, 990
MboI GATC 4 cut(s) 510, 572, 864, 988
MboII GAAGA 6 cut(s) 234, 446, 664, 694, 751, 920
MfeI CAATTG 1 cut(s) 1054
MflI RGATCY 2 cut(s) 572, 864
MhlI GDGCHC 4 cut(s) 404, 549, 594, 624
MluCI AATT 5 cut(s) 924, 955, 1027, 1054, 1090
Mly113I GGCGCC 1 cut(s) 806
MlyI GAGTC 1 cut(s) 671
MmeI TCCRAC 2 cut(s) 33, 280
MroXI GAANNNNTTC 1 cut(s) 927
MseI TTAA 2 cut(s) 944, 1093
MspI CCGG 3 cut(s) 141, 227, 414
MspR9I CCNGG 6 cut(s) 133, 141, 332, 415, 493, 617
MunI CAATTG 1 cut(s) 1054
Mva1269I GAATGC 1 cut(s) 1041
MvaI CCWGG 4 cut(s) 133, 332, 493, 617
MvnI CGCG 4 cut(s) 315, 486, 488, 1041
NarI GGCGCC 1 cut(s) 806
NciI CCSGG 2 cut(s) 141, 415
NcoI CCATGG 1 cut(s) 759
NdeII GATC 4 cut(s) 510, 572, 864, 988
NlaIII CATG 3 cut(s) 385, 763, 898
NlaIV GGNNCC 4 cut(s) 14, 62, 418, 807
NmeAIII GCCGAG 4 cut(s) 370, 379, 739, 775
PciSI GCTCTTC 1 cut(s) 429
PcsI WCGNNNNNNNCGW 2 cut(s) 546, 555
PctI GAATGC 1 cut(s) 1041
PdmI GAANNNNTTC 1 cut(s) 927
PfeI GAWTC 2 cut(s) 205, 612
PfoI TCCNGGA 1 cut(s) 615
PkrI GCNGC 6 cut(s) 314, 412, 490, 748, 778, 793
Ple19I CGATCG 1 cut(s) 513
PleI GAGTC 1 cut(s) 670
PluTI GGCGCC 1 cut(s) 809
PmaCI CACGTG 1 cut(s) 550
PmlI CACGTG 1 cut(s) 550
PpsI GAGTC 1 cut(s) 670
Ppu21I YACGTR 2 cut(s) 325, 550
Psp124BI GAGCTC 3 cut(s) 404, 594, 624
Psp6I CCWGG 4 cut(s) 131, 330, 491, 615
PspCI CACGTG 1 cut(s) 550
PspFI CCCAGC 1 cut(s) 157
PspGI CCWGG 4 cut(s) 131, 330, 491, 615
PspN4I GGNNCC 4 cut(s) 14, 62, 418, 807
PspPI GGNCC 2 cut(s) 143, 417
PsuI RGATCY 2 cut(s) 572, 864
PvuI CGATCG 1 cut(s) 513
RsaI GTAC 4 cut(s) 171, 323, 329, 569
RsaNI GTAC 4 cut(s) 170, 322, 328, 568
SacI GAGCTC 3 cut(s) 404, 594, 624
SapI GCTCTTC 1 cut(s) 429
SaqAI TTAA 2 cut(s) 944, 1093
SatI GCNGC 6 cut(s) 313, 411, 489, 747, 777, 792
Sau3AI GATC 4 cut(s) 510, 572, 864, 988
Sau96I GGNCC 2 cut(s) 143, 417
SchI GAGTC 1 cut(s) 671
ScrFI CCNGG 6 cut(s) 133, 141, 332, 415, 493, 617
SduI GDGCHC 4 cut(s) 404, 549, 594, 624
SfaNI GCATC 1 cut(s) 823
SfoI GGCGCC 1 cut(s) 807
SinI GGWCC 1 cut(s) 417
Sse9I AATT 5 cut(s) 924, 955, 1027, 1054, 1090
SsiI CCGC 9 cut(s) 291, 313, 408, 411, 484, 488, 597, 747, 791
SspDI GGCGCC 1 cut(s) 805
SspMI CTAG 1 cut(s) 1071
SstI GAGCTC 3 cut(s) 404, 594, 624
StyD4I CCNGG 6 cut(s) 131, 139, 330, 413, 491, 615
StyI CCWWGG 3 cut(s) 576, 666, 759
TaaI ACNGT 1 cut(s) 430
TaiI ACGT 3 cut(s) 171, 327, 552
TaqI TCGA 5 cut(s) 179, 509, 513, 703, 1031
TaqII GACCGA 1 cut(s) 434
TasI AATT 5 cut(s) 924, 955, 1027, 1054, 1090
TatI WGTACW 1 cut(s) 567
TauI GCSGC 5 cut(s) 315, 413, 491, 749, 794
TfiI GAWTC 2 cut(s) 205, 612
Tru1I TTAA 2 cut(s) 944, 1093
Tru9I TTAA 2 cut(s) 944, 1093
TscAI CASTG 1 cut(s) 1020
TseI GCWGC 1 cut(s) 776
TspDTI ATGAA 5 cut(s) 370, 701, 920, 962, 1016
TspRI CASTG 1 cut(s) 1020
VpaK11BI GGWCC 1 cut(s) 417
XapI RAATTY 2 cut(s) 924, 1027
XmnI GAANNNNTTC 1 cut(s) 927
XspI CTAG 1 cut(s) 1071
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.