pycom01g10310

phosphatidylinositol 4-phosphate 5-kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0006578
Physical Location & Seq
Forward (+)
11105204 .. 11108809
3606 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1197 bp
ATGGAGCTTCTGCAAGAAGAGCTTTCACCAGATGACTTCAAAGCTACTAAAACAAACTGTATAAAGTATACCAGGCAGGACTCGATACATGGAGCTTACTTCACAAATGACTTTGAATGGAAGGATTACAGTCCTGCAGTTTTCAGACGTTTGCTGGAGCTTGACAGTATAATTAGTTATGATGACTACCTGCTTACAGTATGTGGAGATGACACTCTAAGGGCGGTTTCTTTACCAGGACGGTACGGTAGGGTGTATATATTGCCTGGTGACAAACGCTTAGTGATTAAAACTCTGCGGAAATCAGAAAAGCAGGTGTACCTAGAAATGCTTCCAAACTATTACCGCCATGTTAAGAAGCACGGGGCCTCACTTTTGAAAAGACTGTATGGACTTCATGTTATCAGGCCACCGGGAGGTATCAAGGTTTACTTTGCGGTTTGCGCAAGTGTAATGCCGTCAGAACTTTGCTTATATAAGTGCTATGATCTCAAAGGCTCTTCAGTAGGTAGAGCCCGCAACAGATTACACGTCCACGACACGGCCATACTTAAGGATCTGGATTTTGATTTTTGCTTTTACTTGGATCCATTGGTCCATGCTCGGCTCTTAGCACAAATCAAGTACGACTGCGAGTTTTTGGAGGCCGAGGGCATCATGGATTATAGTTTATTGCTTGGTATGCACATAGATACTTCGCGTTCACGGGAAGAATCCATCTATCCCTTGGCAGGTTCAATTGATGAAAGAAATTCCAGAAGTTTTGATGCCAAAAAGCAAACAGATGATGCTTCTGAAGACGATGAAACCTCATCAGAGTTAACTCTTGCCGATATCTGCGATCTAATTGACAGGCCTGGTTTTAAATTTGGCGACAGACTGCCAGCACGCGCTGTCCGAGCCTTCAATAATGAAATGGAGAGTAAATCATATAGGTCCAGCAGATCACAGGAATGCTTCAATGTTCTTTTGTTCTTTGGAATAATAGACTTTTGTCAGAATTACAATATGAAGAAACGCATTGAGCATGCTTTCAAGGCAATCAAATATGACTCTAAGTCCATCAATACTGTGAACCCCAAAGCCTATTCTTCTCGCTTTCAAGAGTTTCTGAGCGAAGTATTTCTGCCCGAAGAGTCGGATGACTTTGATCTCAACCAGCAGGTTCCTTCTGCTCCTTCAAAATCTGACCCTTAA

Protein Analysis

399

Amino Acids

45.86

Weight (kDa)

5.81

Isoelectric Point (pI)

48.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIP5K PF01504 68 - 373 9.5e-72 Phosphatidylinositol-4-phosphate 5-Kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 304
Acc16I TGCGCA 1 cut(s) 445
Acc36I ACCTGC 4 cut(s) 198, 304, 722, 1153
AccI GTMKAC 1 cut(s) 68
AccII CGCG 2 cut(s) 700, 891
AciI CCGC 5 cut(s) 224, 298, 346, 437, 517
AclWI GGATC 3 cut(s) 564, 581, 594
AcoI YGGCCR 1 cut(s) 543
AcsI RAATTY 2 cut(s) 751, 866
AcuI CTGAAG 2 cut(s) 486, 816
AfaI GTAC 3 cut(s) 245, 320, 626
AfiI CCNNNNNNNGG 3 cut(s) 416, 541, 731
AflII CTTAAG 1 cut(s) 551
AflIII ACRYGT 1 cut(s) 529
AgsI TTSAA 9 cut(s) 40, 116, 379, 738, 907, 961, 1036, 1103, 1182
AhdI GACNNNNNGTC 1 cut(s) 1057
AjiI CACGTC 1 cut(s) 532
AjnI CCWGG 4 cut(s) 71, 235, 265, 856
AluBI AGCT 5 cut(s) 7, 22, 44, 95, 160
AluI AGCT 5 cut(s) 7, 22, 44, 95, 160
AlwI GGATC 3 cut(s) 564, 581, 594
AoxI GGCC 5 cut(s) 366, 407, 543, 645, 854
ApoI RAATTY 2 cut(s) 751, 866
Asp700I GAANNNNTTC 2 cut(s) 330, 1122
AspLEI GCGC 2 cut(s) 446, 893
AspS9I GGNCC 3 cut(s) 366, 595, 936
AsuC2I CCSGG 1 cut(s) 414
AsuHPI GGTGA 2 cut(s) 18, 281
AvaII GGWCC 2 cut(s) 595, 936
BamHI GGATCC 1 cut(s) 586
BanII GRGCYC 1 cut(s) 517
BbsI GAAGAC 1 cut(s) 804
BccI CCATC 2 cut(s) 725, 1070
BceAI ACGGC 2 cut(s) 442, 558
BciT130I CCWGG 4 cut(s) 73, 237, 267, 858
BcnI CCSGG 1 cut(s) 414
BfaI CTAG 1 cut(s) 323
BfmI CTRYAG 1 cut(s) 135
BfrI CTTAAG 1 cut(s) 551
BfuAI ACCTGC 4 cut(s) 198, 304, 722, 1153
Bme1390I CCNGG 5 cut(s) 73, 237, 267, 414, 858
Bme18I GGWCC 2 cut(s) 595, 936
BmeRI GACNNNNNGTC 1 cut(s) 1057
BmgBI CACGTC 1 cut(s) 532
BmgT120I GGNCC 3 cut(s) 366, 595, 936
BmiI GGNNCC 3 cut(s) 367, 588, 1167
BmrFI CCNGG 5 cut(s) 73, 237, 267, 414, 858
BmsI GCATC 3 cut(s) 663, 757, 778
BoxI GACNNNNGTC 1 cut(s) 993
BpiI GAAGAC 1 cut(s) 804
BpmI CTGGAG 1 cut(s) 176
BpuMI CCSGG 1 cut(s) 414
BsaJI CCNNGG 2 cut(s) 648, 726
Bsc4I CCNNNNNNNGG 3 cut(s) 416, 541, 731
BseBI CCWGG 4 cut(s) 73, 237, 267, 858
BseDI CCNNGG 2 cut(s) 648, 726
BseGI GGATG 1 cut(s) 1147
BseLI CCNNNNNNNGG 3 cut(s) 416, 541, 731
BseMII CTCAG 1 cut(s) 1103
Bsh1236I CGCG 2 cut(s) 700, 891
BshFI GGCC 5 cut(s) 368, 409, 545, 647, 856
BsiSI CCGG 1 cut(s) 413
BslI CCNNNNNNNGG 3 cut(s) 416, 541, 731
BsmI GAATGC 1 cut(s) 959
BsnI GGCC 5 cut(s) 368, 409, 545, 647, 856
Bsp1286I GDGCHC 1 cut(s) 517
Bsp143I GATC 6 cut(s) 487, 556, 586, 841, 944, 1150
BspACI CCGC 5 cut(s) 224, 298, 346, 437, 517
BspANI GGCC 5 cut(s) 368, 409, 545, 647, 856
BspCNI CTCAG 1 cut(s) 1104
BspFNI CGCG 2 cut(s) 700, 891
BspLI GGNNCC 3 cut(s) 367, 588, 1167
BspMAI CTGCAG 1 cut(s) 139
BspMI ACCTGC 4 cut(s) 198, 304, 722, 1153
BspPI GGATC 3 cut(s) 564, 581, 594
BspQI GCTCTTC 2 cut(s) 12, 505
BspTI CTTAAG 1 cut(s) 551
BssECI CCNNGG 2 cut(s) 648, 726
BssMI GATC 6 cut(s) 487, 556, 586, 841, 944, 1150
BssNAI GTATAC 1 cut(s) 69
BssT1I CCWWGG 1 cut(s) 726
Bst1107I GTATAC 1 cut(s) 69
Bst2UI CCWGG 4 cut(s) 73, 237, 267, 858
Bst4CI ACNGT 8 cut(s) 59, 131, 167, 199, 243, 248, 387, 1072
Bst6I CTCTTC 3 cut(s) 12, 505, 1128
BstAFI CTTAAG 1 cut(s) 551
BstC8I GCNNGC 4 cut(s) 517, 885, 889, 1029
BstDEI CTNAG 5 cut(s) 218, 280, 610, 1056, 1112
BstF5I GGATG 1 cut(s) 1147
BstFNI CGCG 2 cut(s) 700, 891
BstHHI GCGC 2 cut(s) 446, 893
BstKTI GATC 6 cut(s) 490, 559, 589, 844, 947, 1153
BstMBI GATC 6 cut(s) 487, 556, 586, 841, 944, 1150
BstMWI GCNNNNNNNGC 5 cut(s) 19, 443, 682, 899, 1037
BstNI CCWGG 4 cut(s) 73, 237, 267, 858
BstNSI RCATGY 1 cut(s) 1031
BstPAI GACNNNNGTC 1 cut(s) 993
BstSCI CCNGG 5 cut(s) 71, 235, 265, 412, 856
BstSFI CTRYAG 1 cut(s) 135
BstUI CGCG 2 cut(s) 700, 891
BstV2I GAAGAC 1 cut(s) 804
BstX2I RGATCY 2 cut(s) 556, 586
BstYI RGATCY 2 cut(s) 556, 586
BstZ17I GTATAC 1 cut(s) 69
BsuRI GGCC 5 cut(s) 368, 409, 545, 647, 856
BtrI CACGTC 1 cut(s) 532
BtsCI GGATG 1 cut(s) 1147
BveI ACCTGC 4 cut(s) 198, 304, 722, 1153
Cac8I GCNNGC 4 cut(s) 517, 885, 889, 1029
CfoI GCGC 2 cut(s) 446, 893
Cfr13I GGNCC 3 cut(s) 366, 595, 936
Csp6I GTAC 3 cut(s) 244, 319, 625
CviAII CATG 6 cut(s) 89, 350, 398, 599, 658, 1028
CviQI GTAC 3 cut(s) 244, 319, 625
DdeI CTNAG 5 cut(s) 218, 280, 610, 1056, 1112
DpnI GATC 6 cut(s) 489, 558, 588, 843, 946, 1152
DpnII GATC 6 cut(s) 487, 556, 586, 841, 944, 1150
DraI TTTAAA 1 cut(s) 865
DriI GACNNNNNGTC 1 cut(s) 1057
EaeI YGGCCR 1 cut(s) 543
Eam1104I CTCTTC 3 cut(s) 12, 505, 1128
Eam1105I GACNNNNNGTC 1 cut(s) 1057
EarI CTCTTC 3 cut(s) 12, 505, 1128
Eco130I CCWWGG 1 cut(s) 726
Eco147I AGGCCT 1 cut(s) 856
Eco24I GRGCYC 1 cut(s) 517
Eco32I GATATC 1 cut(s) 835
Eco47I GGWCC 2 cut(s) 595, 936
Eco57I CTGAAG 2 cut(s) 486, 816
EcoO109I RGGNCCY 1 cut(s) 366
EcoRII CCWGG 4 cut(s) 71, 235, 265, 856
EcoRV GATATC 1 cut(s) 835
EcoT14I CCWWGG 1 cut(s) 726
EcoT38I GRGCYC 1 cut(s) 517
ErhI CCWWGG 1 cut(s) 726
FaeI CATG 6 cut(s) 92, 353, 401, 602, 661, 1031
FalI AAGNNNNNCTT 3 cut(s) 38, 416, 448
FatI CATG 6 cut(s) 88, 349, 397, 598, 657, 1027
FauI CCCGC 1 cut(s) 524
FblI GTMKAC 1 cut(s) 68
FokI GGATG 1 cut(s) 1154
FriOI GRGCYC 1 cut(s) 517
FspBI CTAG 1 cut(s) 323
FspI TGCGCA 1 cut(s) 445
GlaI GCGC 2 cut(s) 445, 892
GsuI CTGGAG 1 cut(s) 176
HaeIII GGCC 5 cut(s) 368, 409, 545, 647, 856
HapII CCGG 1 cut(s) 413
HhaI GCGC 2 cut(s) 446, 893
Hin1II CATG 6 cut(s) 92, 353, 401, 602, 661, 1031
Hin6I GCGC 2 cut(s) 444, 891
HinP1I GCGC 2 cut(s) 444, 891
HincII GTYRAC 1 cut(s) 822
HindII GTYRAC 1 cut(s) 822
HinfI GANTC 4 cut(s) 80, 713, 1052, 1136
HpaI GTTAAC 1 cut(s) 822
HpaII CCGG 1 cut(s) 413
HphI GGTGA 2 cut(s) 18, 281
Hpy166II GTNNAC 7 cut(s) 69, 319, 430, 535, 704, 822, 1075
Hpy188III TCNNGA 3 cut(s) 560, 756, 1103
Hpy8I GTNNAC 7 cut(s) 69, 319, 430, 535, 704, 822, 1075
HpyAV CCTTC 4 cut(s) 115, 913, 1179, 1188
HpyCH4III ACNGT 8 cut(s) 59, 131, 167, 199, 243, 248, 387, 1072
HpyCH4IV ACGT 2 cut(s) 148, 531
HpyCH4V TGCA 3 cut(s) 13, 137, 685
HpyF10VI GCNNNNNNNGC 5 cut(s) 19, 443, 682, 899, 1037
HpyF3I CTNAG 5 cut(s) 218, 280, 610, 1056, 1112
HpySE526I ACGT 2 cut(s) 148, 531
Hsp92II CATG 6 cut(s) 92, 353, 401, 602, 661, 1031
HspAI GCGC 2 cut(s) 444, 891
KspAI GTTAAC 1 cut(s) 822
Kzo9I GATC 6 cut(s) 487, 556, 586, 841, 944, 1150
LguI GCTCTTC 2 cut(s) 12, 505
LmnI GCTCC 4 cut(s) 4, 92, 157, 1180
LweI GCATC 3 cut(s) 663, 757, 778
MaeI CTAG 1 cut(s) 323
MaeII ACGT 2 cut(s) 148, 531
MaeIII GTNAC 1 cut(s) 269
MalI GATC 6 cut(s) 489, 558, 588, 843, 946, 1152
MboI GATC 6 cut(s) 487, 556, 586, 841, 944, 1150
MboII GAAGA 7 cut(s) 29, 492, 722, 809, 1024, 1083, 1145
MfeI CAATTG 1 cut(s) 738
MflI RGATCY 2 cut(s) 556, 586
MhlI GDGCHC 1 cut(s) 517
MluCI AATT 6 cut(s) 171, 738, 751, 846, 866, 1000
MlyI GAGTC 3 cut(s) 74, 1046, 1145
MmeI TCCRAC 1 cut(s) 1119
MnlI CCTC 5 cut(s) 379, 410, 637, 643, 820
MroXI GAANNNNTTC 2 cut(s) 330, 1122
MseI TTAA 6 cut(s) 288, 354, 552, 821, 864, 1195
MslI CAYNNNNRTG 1 cut(s) 952
MspCI CTTAAG 1 cut(s) 551
MspI CCGG 1 cut(s) 413
MspR9I CCNGG 5 cut(s) 73, 237, 267, 414, 858
MunI CAATTG 1 cut(s) 738
Mva1269I GAATGC 1 cut(s) 959
MvaI CCWGG 4 cut(s) 73, 237, 267, 858
MvnI CGCG 2 cut(s) 700, 891
MwoI GCNNNNNNNGC 5 cut(s) 19, 443, 682, 899, 1037
NciI CCSGG 1 cut(s) 414
NdeII GATC 6 cut(s) 487, 556, 586, 841, 944, 1150
NlaIII CATG 6 cut(s) 92, 353, 401, 602, 661, 1031
NlaIV GGNNCC 3 cut(s) 367, 588, 1167
NmeAIII GCCGAG 2 cut(s) 583, 673
NmuCI GTSAC 1 cut(s) 269
NsbI TGCGCA 1 cut(s) 445
NspI RCATGY 1 cut(s) 1031
PaeI GCATGC 1 cut(s) 1031
PaqCI CACCTGC 1 cut(s) 304
PceI AGGCCT 1 cut(s) 856
PciSI GCTCTTC 2 cut(s) 12, 505
PcsI WCGNNNNNNNCGW 1 cut(s) 895
PctI GAATGC 1 cut(s) 959
PdmI GAANNNNTTC 2 cut(s) 330, 1122
PfeI GAWTC 1 cut(s) 713
PleI GAGTC 3 cut(s) 74, 1046, 1144
PpsI GAGTC 3 cut(s) 74, 1046, 1144
PshAI GACNNNNGTC 1 cut(s) 993
Psp6I CCWGG 4 cut(s) 71, 235, 265, 856
PspGI CCWGG 4 cut(s) 71, 235, 265, 856
PspN4I GGNNCC 3 cut(s) 367, 588, 1167
PspPI GGNCC 3 cut(s) 366, 595, 936
PstI CTGCAG 1 cut(s) 139
PsuI RGATCY 2 cut(s) 556, 586
RsaI GTAC 3 cut(s) 245, 320, 626
RsaNI GTAC 3 cut(s) 244, 319, 625
RseI CAYNNNNRTG 1 cut(s) 952
SapI GCTCTTC 2 cut(s) 12, 505
SaqAI TTAA 6 cut(s) 288, 354, 552, 821, 864, 1195
Sau3AI GATC 6 cut(s) 487, 556, 586, 841, 944, 1150
Sau96I GGNCC 3 cut(s) 366, 595, 936
SchI GAGTC 3 cut(s) 74, 1046, 1145
ScrFI CCNGG 5 cut(s) 73, 237, 267, 414, 858
SduI GDGCHC 1 cut(s) 517
SfaNI GCATC 3 cut(s) 663, 757, 778
SfcI CTRYAG 1 cut(s) 135
SinI GGWCC 2 cut(s) 595, 936
SmiMI CAYNNNNRTG 1 cut(s) 952
SmlI CTYRAG 1 cut(s) 551
SmoI CTYRAG 1 cut(s) 551
SphI GCATGC 1 cut(s) 1031
Sse9I AATT 6 cut(s) 171, 738, 751, 846, 866, 1000
SseBI AGGCCT 1 cut(s) 856
SsiI CCGC 5 cut(s) 224, 298, 346, 437, 517
SspMI CTAG 1 cut(s) 323
StuI AGGCCT 1 cut(s) 856
StyD4I CCNGG 5 cut(s) 71, 235, 265, 412, 856
StyI CCWWGG 1 cut(s) 726
TaaI ACNGT 8 cut(s) 59, 131, 167, 199, 243, 248, 387, 1072
TaiI ACGT 2 cut(s) 151, 534
TaqI TCGA 1 cut(s) 83
TasI AATT 6 cut(s) 171, 738, 751, 846, 866, 1000
TfiI GAWTC 1 cut(s) 713
Tru1I TTAA 6 cut(s) 288, 354, 552, 821, 864, 1195
Tru9I TTAA 6 cut(s) 288, 354, 552, 821, 864, 1195
TseFI GTSAC 1 cut(s) 269
Tsp45I GTSAC 1 cut(s) 269
TspDTI ATGAA 5 cut(s) 386, 759, 819, 927, 1025
Vha464I CTTAAG 1 cut(s) 551
VpaK11BI GGWCC 2 cut(s) 595, 936
XapI RAATTY 2 cut(s) 751, 866
XceI RCATGY 1 cut(s) 1031
XcmI CCANNNNNNNNNTGG 1 cut(s) 724
XmiI GTMKAC 1 cut(s) 68
XmnI GAANNNNTTC 2 cut(s) 330, 1122
XspI CTAG 1 cut(s) 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.