pycom01g10350

Protein CURVATURE THYLAKOID 1A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0005869
Physical Location & Seq
Reverse (-)
11156788 .. 11159186
2399 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 546 bp
ATGGCAGCGACAGCCTCATCCATGGCGGCCACCGCCGTCTTCATACCACGTTTTCCCATCAAACCCACCCGCTGCTCCGCTTTGCCTTACCTCCCTCCTCGCCTCTCCTCCCCTTCTTCCTTCCCTCTTAAATCCTTCTCAGGGTCCCGGAGGATTTCTCTTCAGGTCAGAGCTTCATCTTCAGAAGAGACATCCACATCCGTCGACACTAATGAACTTATATCAGACTTGAAGGAGAAGTGGGATGCAGTTGAAAACAAGTCTACAGTACTTCTGTATGGAGGTGGGGCGATAGTCGCTGTGTGGTTGTCCTCAATAGTTGTTGGTGCCGTCAATTCAGTGCCACTTCTTCCAAAGGTCCTGGAGTTGGTAGGGCTAGGATACACTGGATGGTTTGTCTACCGTTACCTACTCTTCAAGTCAAGCAGAAAAGAACTAGCTACAGACATTGAGGCACTGAAGAAGAAGATTGCCGGAACTGAATCGATCCGTATTACGAGGTGGCAAACGTCAGGCACATTTATCGTCGACATCCTTTCGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CAAD

Protein Analysis

182

Amino Acids

19.72

Weight (kDa)

9.67

Isoelectric Point (pI)

36.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAAD PF14159 76 - 159 9e-32 CAAD domains of cyanobacterial aminoacyl-tRNA synthetase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 326
AccI GTMKAC 4 cut(s) 204, 263, 399, 528
AciI CCGC 4 cut(s) 26, 33, 70, 78
AclWI GGATC 1 cut(s) 481
AcoI YGGCCR 1 cut(s) 27
AcuI CTGAAG 3 cut(s) 146, 165, 479
AfaI GTAC 1 cut(s) 270
AfiI CCNNNNNNNGG 2 cut(s) 141, 367
AgsI TTSAA 3 cut(s) 232, 254, 418
AjnI CCWGG 1 cut(s) 360
AluBI AGCT 2 cut(s) 173, 440
AluI AGCT 2 cut(s) 173, 440
Alw26I GTCTC 1 cut(s) 182
AlwI GGATC 1 cut(s) 481
AoxI GGCC 1 cut(s) 27
ApeKI GCWGC 2 cut(s) 5, 72
AspS9I GGNCC 2 cut(s) 144, 358
AsuC2I CCSGG 1 cut(s) 148
AvaII GGWCC 2 cut(s) 144, 358
BanI GGYRCC 1 cut(s) 326
BbsI GAAGAC 1 cut(s) 31
BbvI GCAGC 2 cut(s) 17, 59
BccI CCATC 2 cut(s) 65, 384
BceAI ACGGC 2 cut(s) 20, 314
BcgI CGANNNNNNTGC 2 cut(s) 505, 539
BciT130I CCWGG 1 cut(s) 362
BciVI GTATCC 1 cut(s) 374
BcnI CCSGG 1 cut(s) 148
BcoDI GTCTC 1 cut(s) 182
BfaI CTAG 2 cut(s) 377, 437
BfmI CTRYAG 2 cut(s) 264, 441
BfuI GTATCC 1 cut(s) 374
BisI GCNGC 3 cut(s) 6, 27, 73
BlsI GCNGC 3 cut(s) 7, 28, 74
BmcAI AGTACT 1 cut(s) 270
Bme1390I CCNGG 2 cut(s) 148, 362
Bme18I GGWCC 2 cut(s) 144, 358
BmgT120I GGNCC 2 cut(s) 144, 358
BmiI GGNNCC 3 cut(s) 145, 146, 328
BmrFI CCNGG 2 cut(s) 148, 362
BmsI GCATC 1 cut(s) 235
BpiI GAAGAC 1 cut(s) 31
BplI GAGNNNNNCTC 2 cut(s) 142, 174
BpmI CTGGAG 1 cut(s) 383
BpuMI CCSGG 1 cut(s) 148
Bsa29I ATCGAT 1 cut(s) 485
BsaJI CCNNGG 1 cut(s) 21
Bsc4I CCNNNNNNNGG 2 cut(s) 141, 367
Bse1I ACTGG 1 cut(s) 391
BseBI CCWGG 1 cut(s) 362
BseCI ATCGAT 1 cut(s) 485
BseDI CCNNGG 1 cut(s) 21
BseGI GGATG 6 cut(s) 17, 191, 197, 250, 395, 531
BseLI CCNNNNNNNGG 2 cut(s) 141, 367
BseMII CTCAG 1 cut(s) 153
BseNI ACTGG 1 cut(s) 391
BseRI GAGGAG 2 cut(s) 87, 97
BseXI GCAGC 2 cut(s) 17, 59
BshFI GGCC 1 cut(s) 29
BshNI GGYRCC 1 cut(s) 326
BshVI ATCGAT 1 cut(s) 485
BsiSI CCGG 2 cut(s) 148, 474
BslFI GGGAC 1 cut(s) 130
BslI CCNNNNNNNGG 2 cut(s) 141, 367
BsmAI GTCTC 1 cut(s) 182
BsmFI GGGAC 1 cut(s) 130
BsnI GGCC 1 cut(s) 29
Bsp143I GATC 1 cut(s) 486
Bsp19I CCATGG 1 cut(s) 21
BspACI CCGC 4 cut(s) 26, 33, 70, 78
BspANI GGCC 1 cut(s) 29
BspCNI CTCAG 1 cut(s) 152
BspDI ATCGAT 1 cut(s) 485
BspLI GGNNCC 3 cut(s) 145, 146, 328
BspPI GGATC 1 cut(s) 481
BspT107I GGYRCC 1 cut(s) 326
BsrI ACTGG 1 cut(s) 391
BssECI CCNNGG 1 cut(s) 21
BssMI GATC 1 cut(s) 486
BssT1I CCWWGG 1 cut(s) 21
Bst2UI CCWGG 1 cut(s) 362
Bst4CI ACNGT 2 cut(s) 268, 404
Bst6I CTCTTC 3 cut(s) 165, 180, 419
BstDEI CTNAG 1 cut(s) 139
BstDSI CCRYGG 1 cut(s) 21
BstF5I GGATG 6 cut(s) 17, 191, 197, 250, 395, 531
BstKTI GATC 1 cut(s) 489
BstMAI GTCTC 1 cut(s) 182
BstMBI GATC 1 cut(s) 486
BstMWI GCNNNNNNNGC 3 cut(s) 11, 32, 296
BstNI CCWGG 1 cut(s) 362
BstSCI CCNGG 2 cut(s) 146, 360
BstSFI CTRYAG 2 cut(s) 264, 441
BstV1I GCAGC 2 cut(s) 17, 59
BstV2I GAAGAC 1 cut(s) 31
Bsu15I ATCGAT 1 cut(s) 485
BsuI GTATCC 1 cut(s) 374
BsuRI GGCC 1 cut(s) 29
BsuTUI ATCGAT 1 cut(s) 485
BtgI CCRYGG 1 cut(s) 21
BtsCI GGATG 6 cut(s) 17, 191, 197, 250, 395, 531
BtsIMutI CAGTG 3 cut(s) 345, 384, 455
Cfr13I GGNCC 2 cut(s) 144, 358
ClaI ATCGAT 1 cut(s) 485
Csp6I GTAC 1 cut(s) 269
CviAII CATG 1 cut(s) 22
CviJI RGCY 5 cut(s) 14, 29, 173, 376, 440
CviKI_1 RGCY 5 cut(s) 14, 29, 173, 376, 440
CviQI GTAC 1 cut(s) 269
DdeI CTNAG 1 cut(s) 139
DpnI GATC 1 cut(s) 488
DpnII GATC 1 cut(s) 486
EaeI YGGCCR 1 cut(s) 27
Eam1104I CTCTTC 3 cut(s) 165, 180, 419
EarI CTCTTC 3 cut(s) 165, 180, 419
Eco130I CCWWGG 1 cut(s) 21
Eco47I GGWCC 2 cut(s) 144, 358
Eco57I CTGAAG 3 cut(s) 146, 165, 479
EcoO109I RGGNCCY 2 cut(s) 144, 358
EcoRII CCWGG 1 cut(s) 360
EcoT14I CCWWGG 1 cut(s) 21
ErhI CCWWGG 1 cut(s) 21
FaeI CATG 1 cut(s) 25
FaiI YATR 4 cut(s) 23, 44, 221, 279
FaqI GGGAC 1 cut(s) 130
FatI CATG 1 cut(s) 21
FauI CCCGC 1 cut(s) 77
FblI GTMKAC 4 cut(s) 204, 263, 399, 528
Fnu4HI GCNGC 3 cut(s) 6, 27, 73
FokI GGATG 6 cut(s) 4, 178, 184, 257, 402, 518
Fsp4HI GCNGC 3 cut(s) 6, 27, 73
FspBI CTAG 2 cut(s) 377, 437
GluI GCNGC 3 cut(s) 6, 27, 73
GsuI CTGGAG 1 cut(s) 383
HaeIII GGCC 1 cut(s) 29
HapII CCGG 2 cut(s) 148, 474
Hin1II CATG 1 cut(s) 25
HincII GTYRAC 2 cut(s) 205, 529
HindII GTYRAC 2 cut(s) 205, 529
HinfI GANTC 1 cut(s) 482
HpaII CCGG 2 cut(s) 148, 474
Hpy166II GTNNAC 4 cut(s) 205, 264, 400, 529
Hpy188I TCNGA 3 cut(s) 170, 184, 226
Hpy8I GTNNAC 4 cut(s) 205, 264, 400, 529
Hpy99I CGWCG 2 cut(s) 206, 530
HpyAV CCTTC 4 cut(s) 123, 130, 145, 226
HpyCH4III ACNGT 2 cut(s) 268, 404
HpyCH4IV ACGT 2 cut(s) 49, 509
HpyCH4V TGCA 1 cut(s) 248
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 32, 296
HpyF3I CTNAG 1 cut(s) 139
HpySE526I ACGT 2 cut(s) 49, 509
Hsp92II CATG 1 cut(s) 25
KflI GGGWCCC 1 cut(s) 144
Kzo9I GATC 1 cut(s) 486
LmnI GCTCC 1 cut(s) 80
LpnPI CCDG 8 cut(s) 126, 149, 161, 347, 372, 374, 487, 498
Lsp1109I GCAGC 2 cut(s) 17, 59
LweI GCATC 1 cut(s) 235
MaeI CTAG 2 cut(s) 377, 437
MaeII ACGT 2 cut(s) 49, 509
MaeIII GTNAC 1 cut(s) 404
MalI GATC 1 cut(s) 488
MboI GATC 1 cut(s) 486
MluCI AATT 1 cut(s) 334
MseI TTAA 1 cut(s) 129
MspA1I CMGCKG 1 cut(s) 72
MspI CCGG 2 cut(s) 148, 474
MspR9I CCNGG 2 cut(s) 148, 362
MvaI CCWGG 1 cut(s) 362
MwoI GCNNNNNNNGC 3 cut(s) 11, 32, 296
NciI CCSGG 1 cut(s) 148
NcoI CCATGG 1 cut(s) 21
NdeII GATC 1 cut(s) 486
NlaIII CATG 1 cut(s) 25
NlaIV GGNNCC 3 cut(s) 145, 146, 328
PfeI GAWTC 1 cut(s) 482
PfoI TCCNGGA 2 cut(s) 146, 360
PkrI GCNGC 3 cut(s) 7, 28, 74
PpuMI RGGWCCY 2 cut(s) 144, 358
Psp5II RGGWCCY 2 cut(s) 144, 358
Psp6I CCWGG 1 cut(s) 360
PspGI CCWGG 1 cut(s) 360
PspN4I GGNNCC 3 cut(s) 145, 146, 328
PspPI GGNCC 2 cut(s) 144, 358
PspPPI RGGWCCY 2 cut(s) 144, 358
RsaI GTAC 1 cut(s) 270
RsaNI GTAC 1 cut(s) 269
SalI GTCGAC 2 cut(s) 203, 527
SaqAI TTAA 1 cut(s) 129
SatI GCNGC 3 cut(s) 6, 27, 73
Sau3AI GATC 1 cut(s) 486
Sau96I GGNCC 2 cut(s) 144, 358
ScaI AGTACT 1 cut(s) 270
ScrFI CCNGG 2 cut(s) 148, 362
SfaNI GCATC 1 cut(s) 235
SfcI CTRYAG 2 cut(s) 264, 441
SinI GGWCC 2 cut(s) 144, 358
Sse9I AATT 1 cut(s) 334
SsiI CCGC 4 cut(s) 26, 33, 70, 78
SspMI CTAG 2 cut(s) 377, 437
StyD4I CCNGG 2 cut(s) 146, 360
StyI CCWWGG 1 cut(s) 21
TaaI ACNGT 2 cut(s) 268, 404
TaiI ACGT 2 cut(s) 52, 512
TaqI TCGA 3 cut(s) 204, 485, 528
TasI AATT 1 cut(s) 334
TatI WGTACW 1 cut(s) 268
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 1 cut(s) 482
Tru1I TTAA 1 cut(s) 129
Tru9I TTAA 1 cut(s) 129
TscAI CASTG 3 cut(s) 345, 391, 462
TseI GCWGC 2 cut(s) 5, 72
TspDTI ATGAA 3 cut(s) 31, 165, 228
TspGWI ACGGA 2 cut(s) 190, 479
TspRI CASTG 3 cut(s) 345, 391, 462
VpaK11BI GGWCC 2 cut(s) 144, 358
XmiI GTMKAC 4 cut(s) 204, 263, 399, 528
XspI CTAG 2 cut(s) 377, 437
ZrmI AGTACT 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.