pycom01g12550

3-phosphoinositide-dependent protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0006017
Physical Location & Seq
Forward (+)
13283760 .. 13287561
3802 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g12550.4

Sequence Viewer

Length: 1467 bp
ATGTTGGCAATGGAGAAAGATTTTGATGCTAAGTTGAAGATTCAAGGGAATTCTTCTAATGGTGCCAACAGCAATGTTCCGAGATCGATGAGTTTCGCTTTCAGGGCTCCCCAGGAGCATTTCACCATTCAAGACTTTGAATTGGGCAAGATCTATGGCGTTGGTTCCTACTCCAAGGTTGTGAGAGCTAAGAAGAAGGACACTGGAACTGTCTATGCATTAAAGATCATGGACAAAAAATTCATCACCAAAGAAAATAAAACAGCTTATGTGAAGTTGGAACGTATTGTTCTTGATCAGCTGGATCATCCAGGAATTGTGCGGCTATTCTTTACCTTTCAAGATTCCTTTTCATTGTACATGGCACTTGAATCCTGTGAAGGTGGAGAACTTTTTGATCAAATAACTAGGAAAGGTCGTCTCTCTGAGGATGAAGCTCGCTTCTATGCAGCAGAAGTTGTAGACGCACTTGAATACATACATAGTATGGGATTGATTCACAGAGATATAAAGCCAGAGAACTTGCTTCTTACATCAGAGGGTAACATTAAGATTGCTGATTTTGGGAGTGTAAAGCCCATGCAGGATAGCCGAATTACAGTTCTTCCGAATGCAGCATCAGATGATAAGGCCTGCACATTTGTGGGGACAGCTGCTTATGTCCCTCCCGAAGTCCTGAATTCTTCGCCTGCAACTTTCGGAAATGACCTCTGGGCACTTGGTTGCACCTTGTACCAAATGCTTTCAGGAACTTCTCCGTTCAAAGATGCAAGCGAGTGGCTTATTTTCCAAAGAATTATAGCAAGAGATCTTAGGTTTCCAAATAATTTTTCAGAGGAAGCTAAAGGCCTTATCGACCGGTTATTGGATTTAGATCCCAGCAGAAGACCAGGTGCCGGACCGGACGGTTATGCTGCACTGAAGATGCATCCATTCTTTAAGGGAGTTGACTGGAAAAATTTACGAGCACAAACTCCTCCAAAACTGGCTTTGGAGACGGGGTCAGATAAGGGTGATAGCCGCGATAACTCATGGAACCCCTTACACATTGGAGATGGTGCAGCAAAACAAAATGAGGGTAACAGTGGTGCCGCATCTTCTTCTGAAGGATCTGGTCATGTAACTAGGCTTGCTTCAATTGATTCCTTTGATTCCAAATGGCAACAATTCCTGGAGCCTGGAGAGTCGGTTCTTATGATCTCAATGGTGAAGAAAATTCAGAAACTGACAAGCAAGAAGGTGCAGCTTATCCTCACCAACAAGCCAAAATTGATTTATGTGGATCCTGCAAAGCTAATAGTGAAGGGAAATATAATCTGGTCGGACAACTCTAATGACTTCAGCATCCAAGTCGCTAGTCCATCACAGTTCAAGATTTGTACGCCGAAAAAGGTACTGCAATTTGAGGATGCAAAACAAAGAGCTTGGCAGTGGAAGAAGGCAATTGAGGGGCTTCAAAATAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008289 GO:0009893 GO:0009987 GO:0010562 GO:0010604 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019220 GO:0019222 GO:0019538 GO:0023052 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0033674 GO:0035091 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0060255 GO:0065007 GO:0065009 GO:0070300 GO:0071704 GO:0071944 GO:0080090 GO:0140096 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko01524 ko03320 ko04011 ko04068 ko04071 ko04140 ko04150 ko04151 ko04152 ko04210 ko04510 ko04660 ko04664 ko04722 ko04910 ko04919 ko04931 ko04960 ko05145 ko05160 ko05205 ko05213 ko05215 ko05223 ko05231 map01524 map03320 map04011 map04068 map04071 map04140 map04150 map04151 map04152 map04210 map04510 map04660 map04664 map04722 map04910 map04919 map04931 map04960 map05145 map05160 map05205 map05213 map05215 map05223 map05231
Pfam Domains
Protein Families
PH_3 Pkinase

Protein Analysis

489

Amino Acids

54.4

Weight (kDa)

8.71

Isoelectric Point (pI)

37.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 62, 893, 1088
AccI GTMKAC 1 cut(s) 462
AccII CGCG 1 cut(s) 1023
AciI CCGC 3 cut(s) 322, 1021, 1092
AclWI GGATC 5 cut(s) 312, 869, 1117, 1277, 1290
AcsI RAATTY 5 cut(s) 49, 239, 679, 958, 1215
AcuI CTGAAG 3 cut(s) 941, 1125, 1324
AfaI GTAC 4 cut(s) 359, 734, 1381, 1395
AfiI CCNNNNNNNGG 2 cut(s) 865, 896
AgeI ACCGGT 1 cut(s) 858
AjnI CCWGG 5 cut(s) 111, 310, 889, 1170, 1177
AjuI GAANNNNNNNTTGG 2 cut(s) 814, 846
AleI CACNNNNGTG 1 cut(s) 641
AloI GAACNNNNNNTCC 2 cut(s) 1173, 1205
AluBI AGCT 9 cut(s) 188, 266, 301, 437, 653, 842, 1246, 1294, 1424
AluI AGCT 9 cut(s) 188, 266, 301, 437, 653, 842, 1246, 1294, 1424
Alw21I GWGCWC 1 cut(s) 970
Alw26I GTCTC 2 cut(s) 425, 989
AlwI GGATC 5 cut(s) 312, 869, 1117, 1277, 1290
AlwNI CAGNNNCTG 1 cut(s) 1225
AoxI GGCC 2 cut(s) 630, 847
ApeKI GCWGC 6 cut(s) 449, 614, 653, 914, 1061, 1243
ApoI RAATTY 5 cut(s) 49, 239, 679, 958, 1215
AsiGI ACCGGT 1 cut(s) 858
AspS9I GGNCC 1 cut(s) 899
AsuHPI GGTGA 5 cut(s) 115, 238, 1025, 1219, 1246
AvaII GGWCC 1 cut(s) 899
BaeGI GKGCMC 1 cut(s) 718
BamHI GGATCC 1 cut(s) 1282
BanI GGYRCC 3 cut(s) 62, 893, 1088
BanII GRGCYC 1 cut(s) 109
BbsI GAAGAC 1 cut(s) 892
Bbv12I GWGCWC 1 cut(s) 970
BbvI GCAGC 6 cut(s) 461, 626, 640, 901, 1073, 1255
BccI CCATC 2 cut(s) 1049, 1369
BcgI CGANNNNNNTGC 2 cut(s) 1333, 1367
BciT130I CCWGG 5 cut(s) 113, 312, 891, 1172, 1179
BclI TGATCA 2 cut(s) 295, 397
BcoDI GTCTC 2 cut(s) 425, 989
BfaI CTAG 3 cut(s) 408, 1125, 1356
BglII AGATCT 2 cut(s) 150, 808
BisI GCNGC 9 cut(s) 323, 450, 615, 654, 915, 1021, 1062, 1092, 1244
BlsI GCNGC 9 cut(s) 324, 451, 616, 655, 916, 1022, 1063, 1093, 1245
Bme1390I CCNGG 5 cut(s) 113, 312, 891, 1172, 1179
Bme18I GGWCC 1 cut(s) 899
BmgT120I GGNCC 1 cut(s) 899
BmiI GGNNCC 8 cut(s) 64, 108, 166, 895, 1037, 1090, 1176, 1284
BmrFI CCNGG 5 cut(s) 113, 312, 891, 1172, 1179
BmsI GCATC 8 cut(s) 16, 626, 757, 915, 937, 1103, 1353, 1399
BpiI GAAGAC 1 cut(s) 892
BpmI CTGGAG 2 cut(s) 1193, 1200
Bsa29I ATCGAT 1 cut(s) 86
BsaBI GATNNNNATC 1 cut(s) 873
BsaJI CCNNGG 2 cut(s) 111, 174
BsaWI WCCGGW 2 cut(s) 858, 901
BsaXI ACNNNNNCTCC 4 cut(s) 986, 1016, 1173, 1203
Bsc4I CCNNNNNNNGG 2 cut(s) 865, 896
Bse118I RCCGGY 1 cut(s) 858
Bse1I ACTGG 3 cut(s) 208, 956, 990
Bse3DI GCAATG 2 cut(s) 15, 79
Bse8I GATNNNNATC 1 cut(s) 873
BseBI CCWGG 5 cut(s) 113, 312, 891, 1172, 1179
BseCI ATCGAT 1 cut(s) 86
BseDI CCNNGG 2 cut(s) 111, 174
BseGI GGATG 5 cut(s) 307, 436, 928, 1344, 1414
BseJI GATNNNNATC 1 cut(s) 873
BseLI CCNNNNNNNGG 2 cut(s) 865, 896
BseMI GCAATG 2 cut(s) 15, 79
BseMII CTCAG 1 cut(s) 417
BseNI ACTGG 3 cut(s) 208, 956, 990
BseRI GAGGAG 1 cut(s) 966
BseSI GKGCMC 1 cut(s) 718
BseXI GCAGC 6 cut(s) 461, 626, 640, 901, 1073, 1255
BseYI CCCAGC 1 cut(s) 878
BsgI GTGCAG 4 cut(s) 619, 900, 1080, 1262
Bsh1236I CGCG 1 cut(s) 1023
Bsh1285I CGRYCG 1 cut(s) 859
BshFI GGCC 2 cut(s) 632, 849
BshNI GGYRCC 3 cut(s) 62, 893, 1088
BshTI ACCGGT 1 cut(s) 858
BshVI ATCGAT 1 cut(s) 86
BsiEI CGRYCG 1 cut(s) 859
BsiHKAI GWGCWC 1 cut(s) 970
BsiSI CCGG 3 cut(s) 859, 897, 902
BslFI GGGAC 2 cut(s) 647, 661
BslI CCNNNNNNNGG 2 cut(s) 865, 896
BsmAI GTCTC 2 cut(s) 425, 989
BsmBI CGTCTC 2 cut(s) 425, 989
BsmFI GGGAC 2 cut(s) 647, 661
BsmI GAATGC 1 cut(s) 616
BsnI GGCC 2 cut(s) 632, 849
Bsp1286I GDGCHC 3 cut(s) 109, 718, 970
Bsp1407I TGTACA 1 cut(s) 357
BspACI CCGC 3 cut(s) 322, 1021, 1092
BspANI GGCC 2 cut(s) 632, 849
BspCNI CTCAG 1 cut(s) 418
BspDI ATCGAT 1 cut(s) 86
BspFNI CGCG 1 cut(s) 1023
BspLI GGNNCC 8 cut(s) 64, 108, 166, 895, 1037, 1090, 1176, 1284
BspPI GGATC 5 cut(s) 312, 869, 1117, 1277, 1290
BspT107I GGYRCC 3 cut(s) 62, 893, 1088
BsrDI GCAATG 2 cut(s) 15, 79
BsrFI RCCGGY 1 cut(s) 858
BsrGI TGTACA 1 cut(s) 357
BsrI ACTGG 3 cut(s) 208, 956, 990
BssAI RCCGGY 1 cut(s) 858
BssECI CCNNGG 2 cut(s) 111, 174
BssT1I CCWWGG 1 cut(s) 174
Bst2UI CCWGG 5 cut(s) 113, 312, 891, 1172, 1179
Bst4CI ACNGT 5 cut(s) 211, 601, 908, 1085, 1368
BstAUI TGTACA 1 cut(s) 357
BstC8I GCNNGC 5 cut(s) 439, 634, 690, 772, 1131
BstDEI CTNAG 4 cut(s) 30, 189, 426, 812
BstF5I GGATG 5 cut(s) 307, 436, 928, 1344, 1414
BstFNI CGCG 1 cut(s) 1023
BstMAI GTCTC 2 cut(s) 425, 989
BstMCI CGRYCG 1 cut(s) 859
BstMWI GCNNNNNNNGC 1 cut(s) 104
BstNI CCWGG 5 cut(s) 113, 312, 891, 1172, 1179
BstSCI CCNGG 5 cut(s) 111, 310, 889, 1170, 1177
BstSLI GKGCMC 1 cut(s) 718
BstUI CGCG 1 cut(s) 1023
BstV1I GCAGC 6 cut(s) 461, 626, 640, 901, 1073, 1255
BstV2I GAAGAC 1 cut(s) 892
BstX2I RGATCY 5 cut(s) 150, 808, 874, 1109, 1282
BstYI RGATCY 5 cut(s) 150, 808, 874, 1109, 1282
Bsu15I ATCGAT 1 cut(s) 86
BsuRI GGCC 2 cut(s) 632, 849
BsuTUI ATCGAT 1 cut(s) 86
BtsCI GGATG 5 cut(s) 307, 436, 928, 1344, 1414
BtsI GCAGTG 1 cut(s) 1436
BtsIMutI CAGTG 4 cut(s) 201, 917, 1090, 1436
Cac8I GCNNGC 5 cut(s) 439, 634, 690, 772, 1131
CaiI CAGNNNCTG 1 cut(s) 1225
Cfr10I RCCGGY 1 cut(s) 858
Cfr13I GGNCC 1 cut(s) 899
ClaI ATCGAT 1 cut(s) 86
CpoI CGGWCCG 1 cut(s) 899
CseI GACGC 1 cut(s) 473
CsiI ACCWGGT 1 cut(s) 889
Csp6I GTAC 4 cut(s) 358, 733, 1380, 1394
CspAI ACCGGT 1 cut(s) 858
CspI CGGWCCG 1 cut(s) 899
CviAII CATG 5 cut(s) 229, 361, 580, 1032, 1118
CviQI GTAC 4 cut(s) 358, 733, 1380, 1394
DdeI CTNAG 4 cut(s) 30, 189, 426, 812
Eco130I CCWWGG 1 cut(s) 174
Eco147I AGGCCT 2 cut(s) 632, 849
Eco24I GRGCYC 1 cut(s) 109
Eco47I GGWCC 1 cut(s) 899
Eco57I CTGAAG 3 cut(s) 941, 1125, 1324
EcoRI GAATTC 2 cut(s) 49, 679
EcoRII CCWGG 5 cut(s) 111, 310, 889, 1170, 1177
EcoT14I CCWWGG 1 cut(s) 174
EcoT22I ATGCAT 2 cut(s) 220, 930
EcoT38I GRGCYC 1 cut(s) 109
ErhI CCWWGG 1 cut(s) 174
Esp3I CGTCTC 2 cut(s) 425, 989
FaeI CATG 5 cut(s) 232, 364, 583, 1035, 1121
FaqI GGGAC 2 cut(s) 647, 661
FatI CATG 5 cut(s) 228, 360, 579, 1031, 1117
FbaI TGATCA 2 cut(s) 295, 397
FblI GTMKAC 1 cut(s) 462
Fnu4HI GCNGC 9 cut(s) 323, 450, 615, 654, 915, 1021, 1062, 1092, 1244
FokI GGATG 5 cut(s) 294, 443, 915, 1331, 1421
FriOI GRGCYC 1 cut(s) 109
Fsp4HI GCNGC 9 cut(s) 323, 450, 615, 654, 915, 1021, 1062, 1092, 1244
FspBI CTAG 3 cut(s) 408, 1125, 1356
GluI GCNGC 9 cut(s) 323, 450, 615, 654, 915, 1021, 1062, 1092, 1244
GsaI CCCAGC 1 cut(s) 882
GsuI CTGGAG 2 cut(s) 1193, 1200
HaeIII GGCC 2 cut(s) 632, 849
HapII CCGG 3 cut(s) 859, 897, 902
HgaI GACGC 1 cut(s) 473
Hin1II CATG 5 cut(s) 232, 364, 583, 1035, 1121
HincII GTYRAC 1 cut(s) 949
HindII GTYRAC 1 cut(s) 949
HinfI GANTC 7 cut(s) 40, 344, 371, 496, 1142, 1151, 1184
HpaII CCGG 3 cut(s) 859, 897, 902
HphI GGTGA 5 cut(s) 115, 238, 1025, 1219, 1246
Hpy166II GTNNAC 2 cut(s) 463, 949
Hpy188III TCNNGA 7 cut(s) 131, 293, 341, 668, 676, 747, 1372
Hpy8I GTNNAC 2 cut(s) 463, 949
HpyAV CCTTC 6 cut(s) 190, 374, 1100, 1231, 1297, 1432
HpyCH4III ACNGT 5 cut(s) 211, 601, 908, 1085, 1368
HpyCH4IV ACGT 1 cut(s) 283
HpyF10VI GCNNNNNNNGC 1 cut(s) 104
HpyF3I CTNAG 4 cut(s) 30, 189, 426, 812
HpySE526I ACGT 1 cut(s) 283
Hsp92II CATG 5 cut(s) 232, 364, 583, 1035, 1121
Ksp22I TGATCA 2 cut(s) 295, 397
LmnI GCTCC 3 cut(s) 112, 115, 1174
Lsp1109I GCAGC 6 cut(s) 461, 626, 640, 901, 1073, 1255
LweI GCATC 8 cut(s) 16, 626, 757, 915, 937, 1103, 1353, 1399
MabI ACCWGGT 1 cut(s) 889
MaeI CTAG 3 cut(s) 408, 1125, 1356
MaeII ACGT 1 cut(s) 283
MaeIII GTNAC 3 cut(s) 542, 1079, 1120
MfeI CAATTG 2 cut(s) 1137, 1443
MflI RGATCY 5 cut(s) 150, 808, 874, 1109, 1282
MhlI GDGCHC 3 cut(s) 109, 718, 970
MlyI GAGTC 1 cut(s) 1193
MmeI TCCRAC 2 cut(s) 258, 1302
Mph1103I ATGCAT 2 cut(s) 220, 930
MseI TTAA 3 cut(s) 221, 549, 939
MslI CAYNNNNRTG 1 cut(s) 641
MspA1I CMGCKG 2 cut(s) 301, 653
MspI CCGG 3 cut(s) 859, 897, 902
MspR9I CCNGG 5 cut(s) 113, 312, 891, 1172, 1179
MunI CAATTG 2 cut(s) 1137, 1443
Mva1269I GAATGC 1 cut(s) 616
MvaI CCWGG 5 cut(s) 113, 312, 891, 1172, 1179
MvnI CGCG 1 cut(s) 1023
MwoI GCNNNNNNNGC 1 cut(s) 104
NlaIII CATG 5 cut(s) 232, 364, 583, 1035, 1121
NlaIV GGNNCC 8 cut(s) 64, 108, 166, 895, 1037, 1090, 1176, 1284
NsiI ATGCAT 2 cut(s) 220, 930
OliI CACNNNNGTG 1 cut(s) 641
PceI AGGCCT 2 cut(s) 632, 849
PctI GAATGC 1 cut(s) 616
PfeI GAWTC 6 cut(s) 40, 344, 371, 496, 1142, 1151
PflFI GACNNNGTC 1 cut(s) 1000
PfoI TCCNGGA 2 cut(s) 310, 1170
PinAI ACCGGT 1 cut(s) 858
PkrI GCNGC 9 cut(s) 324, 451, 616, 655, 916, 1022, 1063, 1093, 1245
PleI GAGTC 1 cut(s) 1192
PpsI GAGTC 1 cut(s) 1192
Psp6I CCWGG 5 cut(s) 111, 310, 889, 1170, 1177
PspFI CCCAGC 1 cut(s) 878
PspGI CCWGG 5 cut(s) 111, 310, 889, 1170, 1177
PspN4I GGNNCC 8 cut(s) 64, 108, 166, 895, 1037, 1090, 1176, 1284
PspPI GGNCC 1 cut(s) 899
PstNI CAGNNNCTG 1 cut(s) 1225
PsuI RGATCY 5 cut(s) 150, 808, 874, 1109, 1282
PsyI GACNNNGTC 1 cut(s) 1000
PvuII CAGCTG 2 cut(s) 301, 653
RsaI GTAC 4 cut(s) 359, 734, 1381, 1395
RsaNI GTAC 4 cut(s) 358, 733, 1380, 1394
RseI CAYNNNNRTG 1 cut(s) 641
Rsr2I CGGWCCG 1 cut(s) 899
RsrII CGGWCCG 1 cut(s) 899
SaqAI TTAA 3 cut(s) 221, 549, 939
SatI GCNGC 9 cut(s) 323, 450, 615, 654, 915, 1021, 1062, 1092, 1244
Sau96I GGNCC 1 cut(s) 899
SchI GAGTC 1 cut(s) 1193
ScrFI CCNGG 5 cut(s) 113, 312, 891, 1172, 1179
SduI GDGCHC 3 cut(s) 109, 718, 970
SexAI ACCWGGT 1 cut(s) 889
SfaNI GCATC 8 cut(s) 16, 626, 757, 915, 937, 1103, 1353, 1399
SinI GGWCC 1 cut(s) 899
SmiMI CAYNNNNRTG 1 cut(s) 641
SseBI AGGCCT 2 cut(s) 632, 849
SsiI CCGC 3 cut(s) 322, 1021, 1092
SspMI CTAG 3 cut(s) 408, 1125, 1356
StuI AGGCCT 2 cut(s) 632, 849
StyD4I CCNGG 5 cut(s) 111, 310, 889, 1170, 1177
StyI CCWWGG 1 cut(s) 174
TaaI ACNGT 5 cut(s) 211, 601, 908, 1085, 1368
TaiI ACGT 1 cut(s) 286
TaqI TCGA 2 cut(s) 86, 855
TatI WGTACW 1 cut(s) 357
TauI GCSGC 3 cut(s) 325, 1023, 1094
TfiI GAWTC 6 cut(s) 40, 344, 371, 496, 1142, 1151
Tru1I TTAA 3 cut(s) 221, 549, 939
Tru9I TTAA 3 cut(s) 221, 549, 939
TscAI CASTG 4 cut(s) 208, 924, 1090, 1436
TseI GCWGC 6 cut(s) 449, 614, 653, 914, 1061, 1243
TspDTI ATGAA 3 cut(s) 232, 342, 447
TspGWI ACGGA 1 cut(s) 747
TspRI CASTG 4 cut(s) 208, 924, 1090, 1436
Tth111I GACNNNGTC 1 cut(s) 1000
VpaK11BI GGWCC 1 cut(s) 899
XapI RAATTY 5 cut(s) 49, 239, 679, 958, 1215
XmiI GTMKAC 1 cut(s) 462
XspI CTAG 3 cut(s) 408, 1125, 1356
Zsp2I ATGCAT 2 cut(s) 220, 930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.