pycom01g12560
ERF Family

Phosphorylase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0006017
Physical Location & Seq
Forward (+)
13289523 .. 13289930
408 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g12560.1

Sequence Viewer

Length: 351 bp
ATGAAATTCGAAACGCTTCTGAATCAGGTGAATGCTGCTGCAGCAACACAGCAAATGGTGGATCTGTTTGACATTGTAGGAATTGTCCATTTTGGCATTGCCGGAAACGCCAATAATCCCATGGCAATTGGTGATGTATTCCTCAACAGTTTGCTCACGCTGGCATTTGGGATTGGCTGTGGAACTGTGAATTACAATGACGTCGCTGGCTTGGACTTCAAAGGATACAATGTACCAGAAGGAGGAGATAACCTGTTGGGACACATTGGATTTAGTTATGAATTCTTCTCTGAGTCCGGAAAGGCCAACACCGCTGAACCAATTTTTTGGGCAAATACTACTCCCTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNP_UDP_1

Protein Analysis

117

Amino Acids

12.39

Weight (kDa)

4.17

Isoelectric Point (pI)

8.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 204
AccIII TCCGGA 1 cut(s) 296
AciI CCGC 1 cut(s) 312
AclWI GGATC 1 cut(s) 69
AcsI RAATTY 2 cut(s) 5, 281
AcyI GRCGYC 1 cut(s) 201
AfaI GTAC 1 cut(s) 234
AfiI CCNNNNNNNGG 1 cut(s) 242
AgsI TTSAA 1 cut(s) 220
AlwI GGATC 1 cut(s) 69
Aor13HI TCCGGA 1 cut(s) 296
AoxI GGCC 1 cut(s) 303
ApeKI GCWGC 3 cut(s) 35, 38, 41
ApoI RAATTY 2 cut(s) 5, 281
Asp700I GAANNNNTTC 1 cut(s) 15
AsuHPI GGTGA 2 cut(s) 40, 143
AsuII TTCGAA 1 cut(s) 9
BbvI GCAGC 3 cut(s) 22, 25, 53
BciVI GTATCC 1 cut(s) 218
BfmI CTRYAG 1 cut(s) 39
BfuI GTATCC 1 cut(s) 218
BisI GCNGC 3 cut(s) 36, 39, 42
BlsI GCNGC 3 cut(s) 37, 40, 43
Bpu14I TTCGAA 1 cut(s) 9
BsaHI GRCGYC 1 cut(s) 201
BsaJI CCNNGG 1 cut(s) 120
BsaWI WCCGGW 1 cut(s) 296
Bsc4I CCNNNNNNNGG 1 cut(s) 242
Bse3DI GCAATG 1 cut(s) 96
BseAI TCCGGA 1 cut(s) 296
BseDI CCNNGG 1 cut(s) 120
BseLI CCNNNNNNNGG 1 cut(s) 242
BseMI GCAATG 1 cut(s) 96
BseMII CTCAG 1 cut(s) 282
BseRI GAGGAG 1 cut(s) 258
BseXI GCAGC 3 cut(s) 22, 25, 53
BshFI GGCC 1 cut(s) 305
BsiSI CCGG 2 cut(s) 102, 297
BslFI GGGAC 1 cut(s) 273
BslI CCNNNNNNNGG 1 cut(s) 242
BsmFI GGGAC 1 cut(s) 273
BsmI GAATGC 1 cut(s) 37
BsnI GGCC 1 cut(s) 305
Bsp119I TTCGAA 1 cut(s) 9
Bsp13I TCCGGA 1 cut(s) 296
Bsp143I GATC 1 cut(s) 61
Bsp19I CCATGG 1 cut(s) 120
BspACI CCGC 1 cut(s) 312
BspANI GGCC 1 cut(s) 305
BspCNI CTCAG 1 cut(s) 283
BspEI TCCGGA 1 cut(s) 296
BspMAI CTGCAG 1 cut(s) 43
BspPI GGATC 1 cut(s) 69
BspT104I TTCGAA 1 cut(s) 9
BsrDI GCAATG 1 cut(s) 96
BssECI CCNNGG 1 cut(s) 120
BssMI GATC 1 cut(s) 61
BssNI GRCGYC 1 cut(s) 201
BssT1I CCWWGG 1 cut(s) 120
Bst4CI ACNGT 2 cut(s) 149, 187
BstACI GRCGYC 1 cut(s) 201
BstBI TTCGAA 1 cut(s) 9
BstC8I GCNNGC 2 cut(s) 162, 208
BstDEI CTNAG 1 cut(s) 291
BstDSI CCRYGG 1 cut(s) 120
BstKTI GATC 1 cut(s) 64
BstMBI GATC 1 cut(s) 61
BstMWI GCNNNNNNNGC 3 cut(s) 41, 107, 311
BstSFI CTRYAG 1 cut(s) 39
BstV1I GCAGC 3 cut(s) 22, 25, 53
BstX2I RGATCY 1 cut(s) 61
BstXI CCANNNNNNTGG 1 cut(s) 327
BstYI RGATCY 1 cut(s) 61
BsuI GTATCC 1 cut(s) 218
BsuRI GGCC 1 cut(s) 305
BtgI CCRYGG 1 cut(s) 120
Cac8I GCNNGC 2 cut(s) 162, 208
Csp6I GTAC 1 cut(s) 233
CviAII CATG 1 cut(s) 121
CviJI RGCY 3 cut(s) 177, 210, 305
CviKI_1 RGCY 3 cut(s) 177, 210, 305
CviQI GTAC 1 cut(s) 233
DdeI CTNAG 1 cut(s) 291
DpnI GATC 1 cut(s) 63
DpnII GATC 1 cut(s) 61
Eco130I CCWWGG 1 cut(s) 120
EcoRI GAATTC 1 cut(s) 281
EcoT14I CCWWGG 1 cut(s) 120
ErhI CCWWGG 1 cut(s) 120
FaeI CATG 1 cut(s) 124
FaiI YATR 2 cut(s) 122, 279
FaqI GGGAC 1 cut(s) 273
FatI CATG 1 cut(s) 120
Fnu4HI GCNGC 3 cut(s) 36, 39, 42
Fsp4HI GCNGC 3 cut(s) 36, 39, 42
GluI GCNGC 3 cut(s) 36, 39, 42
HaeIII GGCC 1 cut(s) 305
HapII CCGG 2 cut(s) 102, 297
Hin1I GRCGYC 1 cut(s) 201
Hin1II CATG 1 cut(s) 124
HinfI GANTC 2 cut(s) 22, 293
HpaII CCGG 2 cut(s) 102, 297
HphI GGTGA 2 cut(s) 40, 143
Hpy188I TCNGA 2 cut(s) 21, 292
Hpy188III TCNNGA 1 cut(s) 297
Hpy99I CGWCG 1 cut(s) 206
HpyAV CCTTC 1 cut(s) 233
HpyCH4III ACNGT 2 cut(s) 149, 187
HpyCH4IV ACGT 1 cut(s) 201
HpyCH4V TGCA 1 cut(s) 41
HpyF10VI GCNNNNNNNGC 3 cut(s) 41, 107, 311
HpyF3I CTNAG 1 cut(s) 291
HpySE526I ACGT 1 cut(s) 201
Hsp92I GRCGYC 1 cut(s) 201
Hsp92II CATG 1 cut(s) 124
Kpn2I TCCGGA 1 cut(s) 296
Kzo9I GATC 1 cut(s) 61
LpnPI CCDG 7 cut(s) 11, 115, 146, 192, 249, 266, 310
Lsp1109I GCAGC 3 cut(s) 22, 25, 53
MaeII ACGT 1 cut(s) 201
MalI GATC 1 cut(s) 63
MboI GATC 1 cut(s) 61
MboII GAAGA 1 cut(s) 277
MfeI CAATTG 1 cut(s) 126
MflI RGATCY 1 cut(s) 61
MluCI AATT 6 cut(s) 5, 81, 126, 190, 281, 321
MlyI GAGTC 1 cut(s) 302
MnlI CCTC 2 cut(s) 152, 236
MroI TCCGGA 1 cut(s) 296
MroXI GAANNNNTTC 1 cut(s) 15
MseI TTAA 1 cut(s) 349
MspA1I CMGCKG 1 cut(s) 314
MspI CCGG 2 cut(s) 102, 297
MunI CAATTG 1 cut(s) 126
Mva1269I GAATGC 1 cut(s) 37
MwoI GCNNNNNNNGC 3 cut(s) 41, 107, 311
NcoI CCATGG 1 cut(s) 120
NdeII GATC 1 cut(s) 61
NlaIII CATG 1 cut(s) 124
NspV TTCGAA 1 cut(s) 9
PctI GAATGC 1 cut(s) 37
PdmI GAANNNNTTC 1 cut(s) 15
PfeI GAWTC 1 cut(s) 22
PkrI GCNGC 3 cut(s) 37, 40, 43
PleI GAGTC 1 cut(s) 301
PpsI GAGTC 1 cut(s) 301
PstI CTGCAG 1 cut(s) 43
PsuI RGATCY 1 cut(s) 61
RsaI GTAC 1 cut(s) 234
RsaNI GTAC 1 cut(s) 233
SaqAI TTAA 1 cut(s) 349
SatI GCNGC 3 cut(s) 36, 39, 42
Sau3AI GATC 1 cut(s) 61
SchI GAGTC 1 cut(s) 302
SetI ASST 3 cut(s) 30, 204, 255
SfcI CTRYAG 1 cut(s) 39
SfuI TTCGAA 1 cut(s) 9
Sse9I AATT 6 cut(s) 5, 81, 126, 190, 281, 321
SsiI CCGC 1 cut(s) 312
StyI CCWWGG 1 cut(s) 120
TaaI ACNGT 2 cut(s) 149, 187
TaiI ACGT 1 cut(s) 204
TaqI TCGA 1 cut(s) 9
TasI AATT 6 cut(s) 5, 81, 126, 190, 281, 321
TfiI GAWTC 1 cut(s) 22
Tru1I TTAA 1 cut(s) 349
Tru9I TTAA 1 cut(s) 349
TseI GCWGC 3 cut(s) 35, 38, 41
TspDTI ATGAA 2 cut(s) 17, 294
XapI RAATTY 2 cut(s) 5, 281
XcmI CCANNNNNNNNNTGG 1 cut(s) 118
XmnI GAANNNNTTC 1 cut(s) 15
ZraI GACGTC 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.