pycom01g19400

Removes the phosphate from trehalose 6-phosphate to produce free trehalose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0007067
Physical Location & Seq
Forward (+)
18067374 .. 18069519
2146 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1161 bp
ATGGACCTGAAATCAAATCATGCTTCTCCTGTTCTTGCTGACCCTCCACCCTTGAGCAAGTCAAGACTTGGGGGTGTCCATACCAGCCTGTTACCATATGCAACTCCAGGAGCAGCTTTCCCGGCAAGTCTATTCCTAACAATCCCTAGGAGGAAGACTGGATTACTTGATGATGTTCGCTCTAGCTCTTGGCTCGATGCCATGAAATCTTCATCGCCTCCTCACAGAAAGATAACGAAGGGTGTTAACAATGAGTCCGGGGCAACAGATACTGATATTGCTTACCACTCATGGATGGTCAAGTATCCATCAGCGCTTACATCTTTTGAGCAAATCTCTAATCATGCCAAAGGCAAGAGAATAGCACTGTTTCTTGATTATGACGGGACTCTTTCACCGATTGTAGATAACCCTGACTGTGCCTTTATGTCTGATGCCATGCGTGCAGCCGTAAAAAGGGTAGCAAAACATTTCCCAACAGCAATTATTAGTGGAAGAAGCCATGACAAGGTATACGAATTTGTAAAACTAAATGAACTGTATTATGCCGGTAGTCATGGAATGGACATAATGGCCCCTGTTATACAATCTATAAACGATGACCAGAGAAACTGTTTTAAGTCAACTGACAAAAAGGGTAAGGGGGTTAACTTGTTCCAGCCTGCGGCTGAATTTTTACCTATGATTAGAGAGGTATTTAAATTGCTTGTTGAAAGTACGAAAGACATCCAAGGAGCTAAAGTTGAGGACAATAAGTTCTGTGTCTCTGTACATTACCGCAATGTAGATGAGAAGAGTTGGCCTGCAGTTGCGCAATGTGTCCACGAAATTCTTAAGGACTACCCACGCTTGCGCTTGACTCATGGGCGAAAGGTTTTGGAGGTTCGACCTACAATAAACTGGGATAAAGGGAAGGCCGTCACATTTTTACTTGAATCTCTCGGGCTAAGTAATTCCGAAGATGTGCTCCCGATATATATTGGTGACGATCGGACAGATGAAGATGCATTCAAGGTCTTGAGGGAAGGAAACCGAGGCTATGGCATTCTAGTCTCGGCTGTGCCCAAGGACAGCAATGCATTTTACTCTCTAAGGGAACCCTTGGAGGTAATGGAGTTTCTCAAGTCACTGGTTTTATGGAGGAAGTCAAGTGCTCTATAA

Protein Analysis

387

Amino Acids

43.04

Weight (kDa)

8.8

Isoelectric Point (pI)

35.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trehalose_PPase PF02358 124 - 367 2.9e-73 Trehalose-phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 813
AccI GTMKAC 1 cut(s) 513
AciI CCGC 2 cut(s) 665, 778
AcsI RAATTY 3 cut(s) 518, 671, 828
AfaI GTAC 2 cut(s) 718, 771
AfeI AGCGCT 1 cut(s) 315
AfiI CCNNNNNNNGG 3 cut(s) 456, 508, 664
AflII CTTAAG 1 cut(s) 833
AgsI TTSAA 3 cut(s) 713, 935, 1012
AjnI CCWGG 1 cut(s) 106
AloI GAACNNNNNNTCC 2 cut(s) 740, 772
AluBI AGCT 3 cut(s) 116, 186, 737
AluI AGCT 3 cut(s) 116, 186, 737
Alw21I GWGCWC 2 cut(s) 969, 1156
Alw26I GTCTC 2 cut(s) 769, 1057
AlwNI CAGNNNCTG 1 cut(s) 272
Ama87I CYCGRG 1 cut(s) 941
Aor51HI AGCGCT 1 cut(s) 315
AoxI GGCC 3 cut(s) 573, 800, 915
ApeKI GCWGC 2 cut(s) 113, 446
ApoI RAATTY 3 cut(s) 518, 671, 828
AspA2I CCTAGG 1 cut(s) 146
AspLEI GCGC 3 cut(s) 316, 814, 855
AspS9I GGNCC 2 cut(s) 4, 574
AsuC2I CCSGG 2 cut(s) 122, 259
AsuHPI GGTGA 2 cut(s) 387, 995
AvaI CYCGRG 1 cut(s) 941
AvaII GGWCC 1 cut(s) 4
AvrII CCTAGG 1 cut(s) 146
BaeGI GKGCMC 1 cut(s) 1065
BbsI GAAGAC 1 cut(s) 161
Bbv12I GWGCWC 2 cut(s) 969, 1156
BbvI GCAGC 2 cut(s) 125, 458
BccI CCATC 2 cut(s) 289, 316
BceAI ACGGC 2 cut(s) 434, 902
BciT130I CCWGG 1 cut(s) 108
BciVI GTATCC 1 cut(s) 315
BcnI CCSGG 2 cut(s) 122, 259
BcoDI GTCTC 2 cut(s) 769, 1057
BfaI CTAG 3 cut(s) 147, 183, 1049
BfmI CTRYAG 1 cut(s) 804
BfoI RGCGCY 1 cut(s) 317
BfrI CTTAAG 1 cut(s) 833
BfuI GTATCC 1 cut(s) 315
BisI GCNGC 3 cut(s) 114, 447, 666
BlnI CCTAGG 1 cut(s) 146
BlsI GCNGC 3 cut(s) 115, 448, 667
Bme1390I CCNGG 3 cut(s) 108, 122, 259
Bme18I GGWCC 1 cut(s) 4
BmeT110I CYCGRG 1 cut(s) 941
BmgT120I GGNCC 2 cut(s) 4, 574
BmiI GGNNCC 2 cut(s) 576, 1098
BmrFI CCNGG 3 cut(s) 108, 122, 259
BmrI ACTGGG 1 cut(s) 910
BmsI GCATC 3 cut(s) 187, 424, 994
BmuI ACTGGG 1 cut(s) 910
BpiI GAAGAC 1 cut(s) 161
BplI GAGNNNNNCTC 2 cut(s) 320, 352
BpmI CTGGAG 1 cut(s) 90
BpuEI CTTGAG 3 cut(s) 73, 1039, 1106
BpuMI CCSGG 2 cut(s) 122, 259
BsaJI CCNNGG 6 cut(s) 146, 258, 730, 1033, 1065, 1101
BsaXI ACNNNNNCTCC 2 cut(s) 726, 756
Bsc4I CCNNNNNNNGG 3 cut(s) 456, 508, 664
Bse118I RCCGGY 1 cut(s) 548
Bse1I ACTGG 3 cut(s) 163, 905, 1134
Bse3DI GCAATG 3 cut(s) 787, 821, 1081
BseBI CCWGG 1 cut(s) 108
BseDI CCNNGG 6 cut(s) 146, 258, 730, 1033, 1065, 1101
BseGI GGATG 2 cut(s) 300, 726
BseLI CCNNNNNNNGG 3 cut(s) 456, 508, 664
BseMI GCAATG 3 cut(s) 787, 821, 1081
BseNI ACTGG 3 cut(s) 163, 905, 1134
BseRI GAGGAG 1 cut(s) 210
BseSI GKGCMC 1 cut(s) 1065
BseXI GCAGC 2 cut(s) 125, 458
BsgI GTGCAG 1 cut(s) 465
Bsh1285I CGRYCG 1 cut(s) 991
BshFI GGCC 3 cut(s) 575, 802, 917
BsiEI CGRYCG 1 cut(s) 991
BsiHKAI GWGCWC 2 cut(s) 969, 1156
BsiHKCI CYCGRG 1 cut(s) 941
BsiSI CCGG 3 cut(s) 122, 258, 549
BslFI GGGAC 1 cut(s) 400
BslI CCNNNNNNNGG 3 cut(s) 456, 508, 664
BsmAI GTCTC 2 cut(s) 769, 1057
BsmFI GGGAC 1 cut(s) 400
BsmI GAATGC 2 cut(s) 1007, 1044
BsnI GGCC 3 cut(s) 575, 802, 917
BsoBI CYCGRG 1 cut(s) 941
Bsp1286I GDGCHC 3 cut(s) 969, 1065, 1156
Bsp1407I TGTACA 1 cut(s) 769
Bsp143I GATC 1 cut(s) 988
BspACI CCGC 2 cut(s) 665, 778
BspANI GGCC 3 cut(s) 575, 802, 917
BspLI GGNNCC 2 cut(s) 576, 1098
BspMAI CTGCAG 1 cut(s) 808
BspTI CTTAAG 1 cut(s) 833
BsrDI GCAATG 3 cut(s) 787, 821, 1081
BsrFI RCCGGY 1 cut(s) 548
BsrGI TGTACA 1 cut(s) 769
BsrI ACTGG 3 cut(s) 163, 905, 1134
BssAI RCCGGY 1 cut(s) 548
BssECI CCNNGG 6 cut(s) 146, 258, 730, 1033, 1065, 1101
BssMI GATC 1 cut(s) 988
BssNAI GTATAC 1 cut(s) 514
BssT1I CCWWGG 4 cut(s) 146, 730, 1065, 1101
Bst1107I GTATAC 1 cut(s) 514
Bst2UI CCWGG 1 cut(s) 108
Bst4CI ACNGT 4 cut(s) 369, 419, 540, 614
Bst6I CTCTTC 1 cut(s) 788
BstAFI CTTAAG 1 cut(s) 833
BstAUI TGTACA 1 cut(s) 769
BstC8I GCNNGC 4 cut(s) 444, 663, 804, 851
BstDEI CTNAG 2 cut(s) 947, 1091
BstF5I GGATG 2 cut(s) 300, 726
BstH2I RGCGCY 1 cut(s) 317
BstHHI GCGC 3 cut(s) 316, 814, 855
BstKTI GATC 1 cut(s) 991
BstMAI GTCTC 2 cut(s) 769, 1057
BstMBI GATC 1 cut(s) 988
BstMCI CGRYCG 1 cut(s) 991
BstMWI GCNNNNNNNGC 2 cut(s) 122, 443
BstNI CCWGG 1 cut(s) 108
BstSCI CCNGG 3 cut(s) 106, 120, 257
BstSFI CTRYAG 1 cut(s) 804
BstSLI GKGCMC 1 cut(s) 1065
BstV1I GCAGC 2 cut(s) 125, 458
BstV2I GAAGAC 1 cut(s) 161
BstZ17I GTATAC 1 cut(s) 514
BsuI GTATCC 1 cut(s) 315
BsuRI GGCC 3 cut(s) 575, 802, 917
BtgZI GCGATG 1 cut(s) 198
BtsCI GGATG 2 cut(s) 300, 726
BtsIMutI CAGTG 2 cut(s) 365, 1127
Cac8I GCNNGC 4 cut(s) 444, 663, 804, 851
CaiI CAGNNNCTG 1 cut(s) 272
CfoI GCGC 3 cut(s) 316, 814, 855
Cfr10I RCCGGY 1 cut(s) 548
Cfr13I GGNCC 2 cut(s) 4, 574
Csp6I GTAC 2 cut(s) 717, 770
CviAII CATG 8 cut(s) 20, 202, 291, 344, 439, 503, 557, 863
CviQI GTAC 2 cut(s) 717, 770
DdeI CTNAG 2 cut(s) 947, 1091
DpnI GATC 1 cut(s) 990
DpnII GATC 1 cut(s) 988
DraI TTTAAA 1 cut(s) 700
Eam1104I CTCTTC 1 cut(s) 788
EarI CTCTTC 1 cut(s) 788
Eco130I CCWWGG 4 cut(s) 146, 730, 1065, 1101
Eco47I GGWCC 1 cut(s) 4
Eco47III AGCGCT 1 cut(s) 315
Eco88I CYCGRG 1 cut(s) 941
EcoRII CCWGG 1 cut(s) 106
EcoT14I CCWWGG 4 cut(s) 146, 730, 1065, 1101
EcoT22I ATGCAT 2 cut(s) 1009, 1081
ErhI CCWWGG 4 cut(s) 146, 730, 1065, 1101
FaeI CATG 8 cut(s) 23, 205, 294, 347, 442, 506, 560, 866
FaqI GGGAC 1 cut(s) 400
FatI CATG 8 cut(s) 19, 201, 290, 343, 438, 502, 556, 862
FauNDI CATATG 1 cut(s) 97
FblI GTMKAC 1 cut(s) 513
Fnu4HI GCNGC 3 cut(s) 114, 447, 666
FokI GGATG 2 cut(s) 307, 713
Fsp4HI GCNGC 3 cut(s) 114, 447, 666
FspBI CTAG 3 cut(s) 147, 183, 1049
FspI TGCGCA 1 cut(s) 813
GlaI GCGC 3 cut(s) 315, 813, 854
GluI GCNGC 3 cut(s) 114, 447, 666
GsuI CTGGAG 1 cut(s) 90
HaeII RGCGCY 1 cut(s) 317
HaeIII GGCC 3 cut(s) 575, 802, 917
HapII CCGG 3 cut(s) 122, 258, 549
HhaI GCGC 3 cut(s) 316, 814, 855
Hin1II CATG 8 cut(s) 23, 205, 294, 347, 442, 506, 560, 866
Hin6I GCGC 3 cut(s) 314, 812, 853
HinP1I GCGC 3 cut(s) 314, 812, 853
HincII GTYRAC 3 cut(s) 247, 624, 649
HindII GTYRAC 3 cut(s) 247, 624, 649
HinfI GANTC 4 cut(s) 254, 388, 859, 935
HpaI GTTAAC 2 cut(s) 247, 649
HpaII CCGG 3 cut(s) 122, 258, 549
HphI GGTGA 2 cut(s) 387, 995
Hpy166II GTNNAC 5 cut(s) 247, 514, 624, 649, 823
Hpy188I TCNGA 3 cut(s) 433, 958, 993
Hpy188III TCNNGA 4 cut(s) 63, 374, 970, 1018
Hpy8I GTNNAC 5 cut(s) 247, 514, 624, 649, 823
HpyAV CCTTC 3 cut(s) 232, 907, 1019
HpyCH4III ACNGT 4 cut(s) 369, 419, 540, 614
HpyCH4V TGCA 5 cut(s) 101, 446, 806, 1007, 1079
HpyF10VI GCNNNNNNNGC 2 cut(s) 122, 443
HpyF3I CTNAG 2 cut(s) 947, 1091
Hsp92II CATG 8 cut(s) 23, 205, 294, 347, 442, 506, 560, 866
HspAI GCGC 3 cut(s) 314, 812, 853
KspAI GTTAAC 2 cut(s) 247, 649
Kzo9I GATC 1 cut(s) 988
LmnI GCTCC 3 cut(s) 110, 734, 972
Lsp1109I GCAGC 2 cut(s) 125, 458
LweI GCATC 3 cut(s) 187, 424, 994
MaeI CTAG 3 cut(s) 147, 183, 1049
MaeIII GTNAC 4 cut(s) 90, 919, 983, 1125
MalI GATC 1 cut(s) 990
MboI GATC 1 cut(s) 988
MboII GAAGA 6 cut(s) 166, 201, 507, 805, 971, 1013
MhlI GDGCHC 3 cut(s) 969, 1065, 1156
MluCI AATT 6 cut(s) 483, 518, 671, 701, 828, 952
MlyI GAGTC 3 cut(s) 263, 382, 853
Mph1103I ATGCAT 2 cut(s) 1009, 1081
MseI TTAA 5 cut(s) 246, 618, 648, 699, 834
MspCI CTTAAG 1 cut(s) 833
MspI CCGG 3 cut(s) 122, 258, 549
MspR9I CCNGG 3 cut(s) 108, 122, 259
Mva1269I GAATGC 2 cut(s) 1007, 1044
MvaI CCWGG 1 cut(s) 108
MwoI GCNNNNNNNGC 2 cut(s) 122, 443
NciI CCSGG 2 cut(s) 122, 259
NdeI CATATG 1 cut(s) 97
NdeII GATC 1 cut(s) 988
NlaIII CATG 8 cut(s) 23, 205, 294, 347, 442, 506, 560, 866
NlaIV GGNNCC 2 cut(s) 576, 1098
NmeAIII GCCGAG 1 cut(s) 1034
NmuCI GTSAC 3 cut(s) 919, 983, 1125
NsbI TGCGCA 1 cut(s) 813
NsiI ATGCAT 2 cut(s) 1009, 1081
PctI GAATGC 2 cut(s) 1007, 1044
PfeI GAWTC 1 cut(s) 935
PfoI TCCNGGA 1 cut(s) 106
PkrI GCNGC 3 cut(s) 115, 448, 667
Ple19I CGATCG 1 cut(s) 991
PleI GAGTC 3 cut(s) 262, 382, 853
PpsI GAGTC 3 cut(s) 262, 382, 853
Psp6I CCWGG 1 cut(s) 106
PspGI CCWGG 1 cut(s) 106
PspN4I GGNNCC 2 cut(s) 576, 1098
PspPI GGNCC 2 cut(s) 4, 574
PstI CTGCAG 1 cut(s) 808
PstNI CAGNNNCTG 1 cut(s) 272
PvuI CGATCG 1 cut(s) 991
RsaI GTAC 2 cut(s) 718, 771
RsaNI GTAC 2 cut(s) 717, 770
SaqAI TTAA 5 cut(s) 246, 618, 648, 699, 834
SatI GCNGC 3 cut(s) 114, 447, 666
Sau3AI GATC 1 cut(s) 988
Sau96I GGNCC 2 cut(s) 4, 574
SchI GAGTC 3 cut(s) 263, 382, 853
ScrFI CCNGG 3 cut(s) 108, 122, 259
SduI GDGCHC 3 cut(s) 969, 1065, 1156
SfaNI GCATC 3 cut(s) 187, 424, 994
SfcI CTRYAG 1 cut(s) 804
SinI GGWCC 1 cut(s) 4
SmiI ATTTAAAT 1 cut(s) 700
SmlI CTYRAG 4 cut(s) 52, 833, 1018, 1121
SmoI CTYRAG 4 cut(s) 52, 833, 1018, 1121
Sse9I AATT 6 cut(s) 483, 518, 671, 701, 828, 952
SsiI CCGC 2 cut(s) 665, 778
SspMI CTAG 3 cut(s) 147, 183, 1049
StyD4I CCNGG 3 cut(s) 106, 120, 257
StyI CCWWGG 4 cut(s) 146, 730, 1065, 1101
SwaI ATTTAAAT 1 cut(s) 700
TaaI ACNGT 4 cut(s) 369, 419, 540, 614
TaqI TCGA 2 cut(s) 195, 886
TasI AATT 6 cut(s) 483, 518, 671, 701, 828, 952
TatI WGTACW 1 cut(s) 769
TauI GCSGC 1 cut(s) 668
TfiI GAWTC 1 cut(s) 935
Tru1I TTAA 5 cut(s) 246, 618, 648, 699, 834
Tru9I TTAA 5 cut(s) 246, 618, 648, 699, 834
TscAI CASTG 2 cut(s) 372, 1134
TseFI GTSAC 3 cut(s) 919, 983, 1125
TseI GCWGC 2 cut(s) 113, 446
Tsp45I GTSAC 3 cut(s) 919, 983, 1125
TspDTI ATGAA 4 cut(s) 201, 218, 549, 1014
TspRI CASTG 2 cut(s) 372, 1134
Vha464I CTTAAG 1 cut(s) 833
VpaK11BI GGWCC 1 cut(s) 4
XapI RAATTY 3 cut(s) 518, 671, 828
XmaJI CCTAGG 1 cut(s) 146
XmiI GTMKAC 1 cut(s) 513
XspI CTAG 3 cut(s) 147, 183, 1049
Zsp2I ATGCAT 2 cut(s) 1009, 1081
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.