pycom02g04000

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0009820
Physical Location & Seq
Forward (+)
2650205 .. 2653844
3640 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2355 bp
ATGATGGGTGAAAGCTGGCCGGGTGGCGAGAGTTTTAAAAATGTCCTGAAGCGGCTTCAGAAAGCGAGAGAGAGAGAAAGTGAGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGACTTTCTTCTTCTTCTTCCTTTCTTGCTGTTATGGTTCGAGTTCTTTTGCACTGCAAGCTTCTGTCAATGCGTGCTCTTACAGTCTGAGTCGCCGAGTCGAATCGTTCACCGAGTTGGAGAAAAGAGAGTGAAAATGTTGGACGGGCTGCTCGGCCGTGGATTTGCCTCCAAATGCAAAACGCTGATTAAATTGACGAAAACTCGGATCGATGTGATACGGAGGAAGAGGAACGCGACGCAGAAGTTTCTGAAGAAAGATATCGCTGATCTGCTCACCAGTGGCCTTGATATCAATGCTTACGGAAGGGCTGATGGACTCACCGCGGAGGTGATCCTTTCGTCGTGTTATGATTTTGTCGAGCGTTGCTGTGATTTGGTCCTCAAGAATCTTTCAGTTATGCAGAAACAAAGTGAATGCCCAGAGGAATGTAAGGAACCTGTGTCATCGCTGATGATTGCTGCCGCAAGGTTCTCTGATTTACCAGAGTTGCGTGAACTTAGGCAACTATTTCAAGAGAGATACGCGAATGCTCTGGAGTTATTTGTGAATCAAAAGTTTGTTGAGAACATAGCTTCAAAGCCTCCTACACTGGAGAAGAAGGTGCAGTTAATGAAAGACATAGCAGTAGAGTTCTCAATAAATTGGGACTCCAAGGCCTTTGTACAGAGGATGTCGAAACCTCCTTCTGCAATTCCACAGGACAAGCCAAAAACTTACGGGCCTTCCCATGTTAGTGATGATAAAGCCAAATTGTCTACCGGGAAATCTGCTGTTCCAAAAGAAAATAAGCATAATCTTTTTCCAACAGAAAAGATACTTGATTTTAATGATGCTCAGAAATTACACAAGGAGAAGGATGGTGCTGTCTTGAAAAAGGAGGAGCACGTTCATCAAACTAGGCATAAAGCCTTTGGAATAGAACACAAGTCTCTCAGTGCCAGGGAAGATACTGTGGAAAAAGATGGCCTTGGAATTTTATTCGGGGAAAGGCAAGAAGTGGCTTCTCACAAATATGAAGCATGGAATAGAAAGGAGGACGATCACGTTCATCAGACTAGTCATAGACCCTTTGGAAAAGAACACAAATCTCTTAGTGCCAGGGAAGATACTGTCATGGAAAGAGATGGCCACAGAAGTTTGTTCCGGGAAAGGCAACAAGTTGCTTCTCACAAATATGAAGCTTGGAATAGAAAGGAGGATGCTCCCCCGAAGTCAGTCAGATTGGGCAGTTCATCTCAAGGAAATGGACAGGAGAAGCATCATGAGAGGGAGAACAATGTTCCTAAAAGAGAGCATGAAGCTTTACCTTGTGTACAGCCAATTGTTGCTGGACCTCATGTGAAAAGCAACGGTCAAGGCTTATTTGCTGGTGATAATTATGGTGGCCAACACAGTGACGCAAAATTAGCAATTAAAGAAGAAGAGATGCCGAAGTTGAAGCCCCCCTACAATTATGGGGTCCCTCCTCCGTATGTGAAGCCGAATGCCAAAGCAAAAGATCGAAAACATGAAGCCAAGGATGGTTCTGGCAGTAAGCGCATCACTAAGGATCCTGTAGCATACAACATAGCCTTTGTTGACAATATACTTGGAGGGGTAAATATAGGATCTGATGATGAGGATGAGATGCCTGCTGCTGCAAAAGCTAATAAGCATGATCATGGGAAGGATCGTGCTCATCGGGATGATGCATCTAGCAACCACATACCAAAACCAAGATCTTCCAGGGCAAGAAGCTCAAGATCACGGTCCTCTCACAATGATGCTGGAAACAGTGAAGATACAGAAGTTGCAATCAGAAAAACAAGAAGCAGGAGAAAGGATGAGTCACGACGAGGTCTGCAACTGTTGTTTGATGACGAGCACTACAAAAAGGATGAGGAAGAGAGGAGAATGGACGAGCTTCTGATTCATTACAGTAAAAAGCCATCTAACGTTGAACCAGAAATGTCAAGAAGAAAGTCGAGGAGTCGTCATGCACATCATCATGCCGGCACTGGTGTTAAAGAATCCCCAAGACAAGCAAGCAGAGATGAGTCGGAAACGGTTCCTACAGCAGTAAGATCAGTTTCCCTTCCTAATGAACATAGTGGTCCATCAGAGGCACCCAAGGTATTTGCTCGTGCGGCGTCCTTTCAACCCGACAGGTCCAATCCAGCTAAGCATGTGCATCCTAAGCTTCCAGACTACGAAGATTTGGCCGCCCAATTTGCAGCCTTAAGAGGAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

785

Amino Acids

89.15

Weight (kDa)

9.22

Isoelectric Point (pI)

55.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 100 - 255 5.6e-42 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0009820)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32350 AT4G32350 AT4G32350
fragaria_vesca FvH4_1g04930 FvH4_3g42832
malus_domestica MD02G1047800.v1.1 MD15G1186300.v1.1
prunus_persica Prupe.7G230400_v2.0.a1
pyrus_communis pycom02g04000 pycom15g16700
rosa_chinensis RchiOBHm_Chr2g0090521
rosa_laevigata RLG00000016119
rosa_multiflora Rmu_co8330093.1_g000001 Rmu_sc0004514.1_g000026
rosa_roxburghii Rroxscaffold_2G00151050
rosa_rugosa Rorug02G0008800
rosa_samantha Rh2AG054000 Rh2BG053500 Rh2CG055700 Rh2DG054200
rosa_wichuraiana Rw2G004780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 2227
AccI GTMKAC 1 cut(s) 878
AccII CGCG 3 cut(s) 356, 446, 647
AciI CCGC 6 cut(s) 52, 444, 446, 585, 2249, 2325
AclI AACGTT 1 cut(s) 2058
AclWI GGATC 6 cut(s) 335, 448, 1667, 1680, 1738, 1800
AcoI YGGCCR 5 cut(s) 17, 274, 1249, 1507, 2322
AcsI RAATTY 1 cut(s) 1095
AcuI CTGAAG 3 cut(s) 41, 68, 392
AcyI GRCGYC 1 cut(s) 2252
AfaI GTAC 2 cut(s) 786, 1437
AflII CTTAAG 1 cut(s) 2341
AgsI TTSAA 6 cut(s) 635, 699, 994, 1561, 2063, 2261
AhlI ACTAGT 1 cut(s) 1178
AjnI CCWGG 3 cut(s) 1061, 1220, 1847
Alw21I GWGCWC 4 cut(s) 198, 1008, 1801, 1989
Alw26I GTCTC 2 cut(s) 114, 1056
AlwI GGATC 6 cut(s) 335, 448, 1667, 1680, 1738, 1800
AoxI GGCC 9 cut(s) 17, 274, 403, 777, 842, 1087, 1249, 1507, 2322
ApeKI GCWGC 5 cut(s) 268, 581, 1757, 1760, 2336
ApoI RAATTY 1 cut(s) 1095
Asp700I GAANNNNTTC 1 cut(s) 2169
AspLEI GCGC 1 cut(s) 1662
AspS9I GGNCC 7 cut(s) 499, 842, 1454, 1582, 1872, 2216, 2271
AsuC2I CCSGG 3 cut(s) 21, 883, 1268
AsuHPI GGTGA 6 cut(s) 20, 221, 388, 433, 463, 1505
AvaII GGWCC 6 cut(s) 499, 1454, 1582, 1872, 2216, 2271
BaeI ACNNNNGTAYC 2 cut(s) 1896, 1929
BalI TGGCCA 2 cut(s) 1251, 1509
BamHI GGATCC 1 cut(s) 1672
BanI GGYRCC 1 cut(s) 2227
BarI GAAGNNNNNNTAC 2 cut(s) 1553, 1585
BauI CACGAG 1 cut(s) 2244
Bbv12I GWGCWC 4 cut(s) 198, 1008, 1801, 1989
BbvI GCAGC 5 cut(s) 255, 568, 1744, 1747, 2348
BccI CCATC 7 cut(s) 428, 974, 1079, 1241, 1637, 2059, 2227
BceAI ACGGC 1 cut(s) 261
BciT130I CCWGG 3 cut(s) 1063, 1222, 1849
BclI TGATCA 1 cut(s) 1780
BcnI CCSGG 3 cut(s) 21, 883, 1268
BcoDI GTCTC 2 cut(s) 114, 1056
BcuI ACTAGT 1 cut(s) 1178
BfaI CTAG 3 cut(s) 1020, 1179, 1818
BfmI CTRYAG 2 cut(s) 1677, 2175
BfrI CTTAAG 1 cut(s) 2341
BglII AGATCT 1 cut(s) 1841
BisI GCNGC 9 cut(s) 53, 269, 582, 585, 1758, 1761, 2250, 2325, 2337
BlpI GCTNAGC 1 cut(s) 2283
BlsI GCNGC 9 cut(s) 54, 270, 583, 586, 1759, 1762, 2251, 2326, 2338
Bme1390I CCNGG 6 cut(s) 21, 883, 1063, 1222, 1268, 1849
Bme18I GGWCC 6 cut(s) 499, 1454, 1582, 1872, 2216, 2271
BmgT120I GGNCC 7 cut(s) 499, 842, 1454, 1582, 1872, 2216, 2271
BmiI GGNNCC 6 cut(s) 558, 1583, 1584, 1674, 2172, 2229
BmrFI CCNGG 6 cut(s) 21, 883, 1063, 1222, 1268, 1849
BpmI CTGGAG 2 cut(s) 677, 734
Bpu10I CCTNAGC 1 cut(s) 2298
Bpu1102I GCTNAGC 1 cut(s) 2283
BpuEI CTTGAG 3 cut(s) 488, 1344, 1846
BpuMI CCSGG 3 cut(s) 21, 883, 1268
Bsa29I ATCGAT 1 cut(s) 330
BsaHI GRCGYC 1 cut(s) 2252
BsaJI CCNNGG 9 cut(s) 277, 444, 774, 1062, 1090, 1221, 1638, 1848, 2232
Bse118I RCCGGY 1 cut(s) 2114
Bse1I ACTGG 3 cut(s) 399, 717, 2125
BseBI CCWGG 3 cut(s) 1063, 1222, 1849
BseCI ATCGAT 1 cut(s) 330
BseDI CCNNGG 9 cut(s) 277, 444, 774, 1062, 1090, 1221, 1638, 1848, 2232
BseGI GGATG 9 cut(s) 798, 985, 1327, 1648, 1750, 1813, 1951, 2005, 2293
BseMII CTCAG 3 cut(s) 198, 971, 1069
BseNI ACTGG 3 cut(s) 399, 717, 2125
BseRI GAGGAG 5 cut(s) 98, 1016, 1578, 2026, 2104
BseX3I CGGCCG 1 cut(s) 274
BseXI GCAGC 5 cut(s) 255, 568, 1744, 1747, 2348
BsgI GTGCAG 1 cut(s) 746
Bsh1236I CGCG 3 cut(s) 356, 446, 647
Bsh1285I CGRYCG 1 cut(s) 277
BshFI GGCC 9 cut(s) 19, 276, 405, 779, 844, 1089, 1251, 1509, 2324
BshNI GGYRCC 1 cut(s) 2227
BshVI ATCGAT 1 cut(s) 330
BsiEI CGRYCG 1 cut(s) 277
BsiHKAI GWGCWC 4 cut(s) 198, 1008, 1801, 1989
BsiSI CCGG 4 cut(s) 20, 882, 1267, 2115
BslFI GGGAC 2 cut(s) 782, 1568
BsmAI GTCTC 2 cut(s) 114, 1056
BsmFI GGGAC 2 cut(s) 782, 1568
BsmI GAATGC 3 cut(s) 542, 655, 1612
BsnI GGCC 9 cut(s) 19, 276, 405, 779, 844, 1089, 1251, 1509, 2324
Bsp1286I GDGCHC 4 cut(s) 198, 1008, 1801, 1989
Bsp1407I TGTACA 2 cut(s) 784, 1435
Bsp1720I GCTNAGC 1 cut(s) 2283
BspACI CCGC 6 cut(s) 52, 444, 446, 585, 2249, 2325
BspANI GGCC 9 cut(s) 19, 276, 405, 779, 844, 1089, 1251, 1509, 2324
BspCNI CTCAG 3 cut(s) 199, 970, 1068
BspDI ATCGAT 1 cut(s) 330
BspFNI CGCG 3 cut(s) 356, 446, 647
BspHI TCATGA 1 cut(s) 1384
BspLI GGNNCC 6 cut(s) 558, 1583, 1584, 1674, 2172, 2229
BspPI GGATC 6 cut(s) 335, 448, 1667, 1680, 1738, 1800
BspT107I GGYRCC 1 cut(s) 2227
BspTI CTTAAG 1 cut(s) 2341
BsrFI RCCGGY 1 cut(s) 2114
BsrGI TGTACA 2 cut(s) 784, 1435
BsrI ACTGG 3 cut(s) 399, 717, 2125
BssAI RCCGGY 1 cut(s) 2114
BssECI CCNNGG 9 cut(s) 277, 444, 774, 1062, 1090, 1221, 1638, 1848, 2232
BssNI GRCGYC 1 cut(s) 2252
BssSI CACGAG 1 cut(s) 2244
BssT1I CCWWGG 4 cut(s) 774, 1090, 1638, 2232
Bst2BI CACGAG 1 cut(s) 2244
Bst2UI CCWGG 3 cut(s) 1063, 1222, 1849
Bst6I CTCTTC 3 cut(s) 341, 1539, 2001
BstACI GRCGYC 1 cut(s) 2252
BstAFI CTTAAG 1 cut(s) 2341
BstAUI TGTACA 2 cut(s) 784, 1435
BstC8I GCNNGC 6 cut(s) 17, 178, 194, 1755, 2116, 2149
BstDEI CTNAG 8 cut(s) 207, 620, 957, 1055, 1214, 1668, 2283, 2298
BstDSI CCRYGG 2 cut(s) 277, 444
BstF5I GGATG 9 cut(s) 798, 985, 1327, 1648, 1750, 1813, 1951, 2005, 2293
BstFNI CGCG 3 cut(s) 356, 446, 647
BstHHI GCGC 1 cut(s) 1662
BstMAI GTCTC 2 cut(s) 114, 1056
BstMCI CGRYCG 1 cut(s) 277
BstMWI GCNNNNNNNGC 7 cut(s) 177, 1529, 1659, 1766, 2249, 2299, 2333
BstNI CCWGG 3 cut(s) 1063, 1222, 1849
BstNSI RCATGY 1 cut(s) 2291
BstSCI CCNGG 6 cut(s) 19, 881, 1061, 1220, 1266, 1847
BstSFI CTRYAG 2 cut(s) 1677, 2175
BstUI CGCG 3 cut(s) 356, 446, 647
BstV1I GCAGC 5 cut(s) 255, 568, 1744, 1747, 2348
BstX2I RGATCY 3 cut(s) 1672, 1730, 1841
BstYI RGATCY 3 cut(s) 1672, 1730, 1841
BstZI CGGCCG 1 cut(s) 274
Bsu15I ATCGAT 1 cut(s) 330
BsuRI GGCC 9 cut(s) 19, 276, 405, 779, 844, 1089, 1251, 1509, 2324
BsuTUI ATCGAT 1 cut(s) 330
BtgI CCRYGG 2 cut(s) 277, 444
BtgZI GCGATG 1 cut(s) 552
BtsCI GGATG 9 cut(s) 798, 985, 1327, 1648, 1750, 1813, 1951, 2005, 2293
BtsI GCAGTG 1 cut(s) 171
BtsIMutI CAGTG 7 cut(s) 171, 406, 710, 1063, 1522, 1903, 2118
Cac8I GCNNGC 6 cut(s) 17, 178, 194, 1755, 2116, 2149
CciI TCATGA 1 cut(s) 1384
CfoI GCGC 1 cut(s) 1662
Cfr10I RCCGGY 1 cut(s) 2114
Cfr13I GGNCC 7 cut(s) 499, 842, 1454, 1582, 1872, 2216, 2271
Cfr42I CCGCGG 1 cut(s) 447
ClaI ATCGAT 1 cut(s) 330
CseI GACGC 3 cut(s) 367, 1529, 2241
Csp6I GTAC 2 cut(s) 785, 1436
CviQI GTAC 2 cut(s) 785, 1436
DdeI CTNAG 8 cut(s) 207, 620, 957, 1055, 1214, 1668, 2283, 2298
DraI TTTAAA 1 cut(s) 37
EaeI YGGCCR 5 cut(s) 17, 274, 1249, 1507, 2322
EagI CGGCCG 1 cut(s) 274
Eam1104I CTCTTC 3 cut(s) 341, 1539, 2001
EarI CTCTTC 3 cut(s) 341, 1539, 2001
EclXI CGGCCG 1 cut(s) 274
Eco130I CCWWGG 4 cut(s) 774, 1090, 1638, 2232
Eco147I AGGCCT 1 cut(s) 779
Eco32I GATATC 2 cut(s) 382, 412
Eco47I GGWCC 6 cut(s) 499, 1454, 1582, 1872, 2216, 2271
Eco52I CGGCCG 1 cut(s) 274
Eco57I CTGAAG 3 cut(s) 41, 68, 392
EcoO109I RGGNCCY 1 cut(s) 1582
EcoRII CCWGG 3 cut(s) 1061, 1220, 1847
EcoRV GATATC 2 cut(s) 382, 412
EcoT14I CCWWGG 4 cut(s) 774, 1090, 1638, 2232
EcoT22I ATGCAT 1 cut(s) 1816
ErhI CCWWGG 4 cut(s) 774, 1090, 1638, 2232
FalI AAGNNNNNCTT 2 cut(s) 1074, 1106
FaqI GGGAC 2 cut(s) 782, 1568
FbaI TGATCA 1 cut(s) 1780
FblI GTMKAC 1 cut(s) 878
Fnu4HI GCNGC 9 cut(s) 53, 269, 582, 585, 1758, 1761, 2250, 2325, 2337
FokI GGATG 9 cut(s) 805, 992, 1334, 1655, 1757, 1820, 1958, 2012, 2280
Fsp4HI GCNGC 9 cut(s) 53, 269, 582, 585, 1758, 1761, 2250, 2325, 2337
FspBI CTAG 3 cut(s) 1020, 1179, 1818
GlaI GCGC 1 cut(s) 1661
GluI GCNGC 9 cut(s) 53, 269, 582, 585, 1758, 1761, 2250, 2325, 2337
GsuI CTGGAG 2 cut(s) 677, 734
HaeIII GGCC 9 cut(s) 19, 276, 405, 779, 844, 1089, 1251, 1509, 2324
HapII CCGG 4 cut(s) 20, 882, 1267, 2115
HgaI GACGC 3 cut(s) 367, 1529, 2241
HhaI GCGC 1 cut(s) 1662
Hin1I GRCGYC 1 cut(s) 2252
Hin6I GCGC 1 cut(s) 1660
HinP1I GCGC 1 cut(s) 1660
HincII GTYRAC 1 cut(s) 1702
HindII GTYRAC 1 cut(s) 1702
HindIII AAGCTT 4 cut(s) 178, 1302, 1422, 2300
HpaII CCGG 4 cut(s) 20, 882, 1267, 2115
HphI GGTGA 6 cut(s) 20, 221, 388, 433, 463, 1505
Hpy166II GTNNAC 5 cut(s) 229, 617, 879, 1436, 1702
Hpy8I GTNNAC 5 cut(s) 229, 617, 879, 1436, 1702
Hpy99I CGWCG 3 cut(s) 361, 466, 1959
HpyAV CCTTC 8 cut(s) 420, 715, 816, 855, 970, 1783, 2207, 2343
HpyCH4IV ACGT 3 cut(s) 1008, 1167, 2058
HpyF10VI GCNNNNNNNGC 7 cut(s) 177, 1529, 1659, 1766, 2249, 2299, 2333
HpyF3I CTNAG 8 cut(s) 207, 620, 957, 1055, 1214, 1668, 2283, 2298
HpySE526I ACGT 3 cut(s) 1008, 1167, 2058
Hsp92I GRCGYC 1 cut(s) 2252
HspAI GCGC 1 cut(s) 1660
KflI GGGWCCC 1 cut(s) 1582
KroI GCCGGC 1 cut(s) 2114
KroNI GCCGGC 1 cut(s) 2116
Ksp22I TGATCA 1 cut(s) 1780
KspI CCGCGG 1 cut(s) 447
LmnI GCTCC 2 cut(s) 1003, 1330
Lsp1109I GCAGC 5 cut(s) 255, 568, 1744, 1747, 2348
MaeI CTAG 3 cut(s) 1020, 1179, 1818
MaeII ACGT 3 cut(s) 1008, 1167, 2058
MaeIII GTNAC 2 cut(s) 1517, 1950
MfeI CAATTG 1 cut(s) 1443
MflI RGATCY 3 cut(s) 1672, 1730, 1841
MhlI GDGCHC 4 cut(s) 198, 1008, 1801, 1989
MlsI TGGCCA 2 cut(s) 1251, 1509
MluNI TGGCCA 2 cut(s) 1251, 1509
MlyI GAGTC 7 cut(s) 218, 226, 432, 764, 1958, 2101, 2168
MmeI TCCRAC 4 cut(s) 217, 240, 950, 2142
Mox20I TGGCCA 2 cut(s) 1251, 1509
Mph1103I ATGCAT 1 cut(s) 1816
MroNI GCCGGC 1 cut(s) 2114
MroXI GAANNNNTTC 1 cut(s) 2169
MscI TGGCCA 2 cut(s) 1251, 1509
MseI TTAA 7 cut(s) 36, 309, 731, 948, 1536, 2127, 2342
MslI CAYNNNNRTG 7 cut(s) 855, 1134, 1296, 1782, 1806, 1884, 2109
Msp20I TGGCCA 2 cut(s) 1251, 1509
MspA1I CMGCKG 1 cut(s) 446
MspCI CTTAAG 1 cut(s) 2341
MspI CCGG 4 cut(s) 20, 882, 1267, 2115
MspR9I CCNGG 6 cut(s) 21, 883, 1063, 1222, 1268, 1849
MunI CAATTG 1 cut(s) 1443
Mva1269I GAATGC 3 cut(s) 542, 655, 1612
MvaI CCWGG 3 cut(s) 1063, 1222, 1849
MvnI CGCG 3 cut(s) 356, 446, 647
MwoI GCNNNNNNNGC 7 cut(s) 177, 1529, 1659, 1766, 2249, 2299, 2333
NaeI GCCGGC 1 cut(s) 2116
NciI CCSGG 3 cut(s) 21, 883, 1268
NgoMIV GCCGGC 1 cut(s) 2114
NlaIV GGNNCC 6 cut(s) 558, 1583, 1584, 1674, 2172, 2229
NmeAIII GCCGAG 2 cut(s) 240, 252
NmuCI GTSAC 2 cut(s) 1517, 1950
NsiI ATGCAT 1 cut(s) 1816
NspI RCATGY 1 cut(s) 2291
PagI TCATGA 1 cut(s) 1384
PceI AGGCCT 1 cut(s) 779
PctI GAATGC 3 cut(s) 542, 655, 1612
PdiI GCCGGC 1 cut(s) 2116
PdmI GAANNNNTTC 1 cut(s) 2169
PfeI GAWTC 5 cut(s) 222, 508, 670, 2032, 2132
PflFI GACNNNGTC 1 cut(s) 1959
PfoI TCCNGGA 1 cut(s) 1266
PkrI GCNGC 9 cut(s) 54, 270, 583, 586, 1759, 1762, 2251, 2326, 2338
PleI GAGTC 7 cut(s) 217, 225, 432, 764, 1957, 2100, 2167
PpsI GAGTC 7 cut(s) 217, 225, 432, 764, 1957, 2100, 2167
PpuMI RGGWCCY 1 cut(s) 1582
Psp1406I AACGTT 1 cut(s) 2058
Psp5II RGGWCCY 1 cut(s) 1582
Psp6I CCWGG 3 cut(s) 1061, 1220, 1847
PspGI CCWGG 3 cut(s) 1061, 1220, 1847
PspN4I GGNNCC 6 cut(s) 558, 1583, 1584, 1674, 2172, 2229
PspPI GGNCC 7 cut(s) 499, 842, 1454, 1582, 1872, 2216, 2271
PspPPI RGGWCCY 1 cut(s) 1582
PsuI RGATCY 3 cut(s) 1672, 1730, 1841
PsyI GACNNNGTC 1 cut(s) 1959
RsaI GTAC 2 cut(s) 786, 1437
RsaNI GTAC 2 cut(s) 785, 1436
RseI CAYNNNNRTG 7 cut(s) 855, 1134, 1296, 1782, 1806, 1884, 2109
SacII CCGCGG 1 cut(s) 447
SaqAI TTAA 7 cut(s) 36, 309, 731, 948, 1536, 2127, 2342
SatI GCNGC 9 cut(s) 53, 269, 582, 585, 1758, 1761, 2250, 2325, 2337
Sau96I GGNCC 7 cut(s) 499, 842, 1454, 1582, 1872, 2216, 2271
SchI GAGTC 7 cut(s) 218, 226, 432, 764, 1958, 2101, 2168
ScrFI CCNGG 6 cut(s) 21, 883, 1063, 1222, 1268, 1849
SduI GDGCHC 4 cut(s) 198, 1008, 1801, 1989
SfcI CTRYAG 2 cut(s) 1677, 2175
Sfr303I CCGCGG 1 cut(s) 447
SgrBI CCGCGG 1 cut(s) 447
SinI GGWCC 6 cut(s) 499, 1454, 1582, 1872, 2216, 2271
SmiMI CAYNNNNRTG 7 cut(s) 855, 1134, 1296, 1782, 1806, 1884, 2109
SmlI CTYRAG 4 cut(s) 503, 1359, 1861, 2341
SmoI CTYRAG 4 cut(s) 503, 1359, 1861, 2341
SpeI ACTAGT 1 cut(s) 1178
SseBI AGGCCT 1 cut(s) 779
SsiI CCGC 6 cut(s) 52, 444, 446, 585, 2249, 2325
SspMI CTAG 3 cut(s) 1020, 1179, 1818
StuI AGGCCT 1 cut(s) 779
StyD4I CCNGG 6 cut(s) 19, 881, 1061, 1220, 1266, 1847
StyI CCWWGG 4 cut(s) 774, 1090, 1638, 2232
TaiI ACGT 3 cut(s) 1011, 1170, 2061
TaqI TCGA 7 cut(s) 159, 220, 330, 480, 797, 1624, 2087
TatI WGTACW 2 cut(s) 784, 1435
TauI GCSGC 4 cut(s) 55, 587, 2252, 2327
TfiI GAWTC 5 cut(s) 222, 508, 670, 2032, 2132
Tru1I TTAA 7 cut(s) 36, 309, 731, 948, 1536, 2127, 2342
Tru9I TTAA 7 cut(s) 36, 309, 731, 948, 1536, 2127, 2342
TscAI CASTG 7 cut(s) 178, 406, 717, 1063, 1522, 1903, 2125
TseFI GTSAC 2 cut(s) 1517, 1950
TseI GCWGC 5 cut(s) 268, 581, 1757, 1760, 2336
Tsp45I GTSAC 2 cut(s) 1517, 1950
TspGWI ACGGA 3 cut(s) 355, 438, 1581
TspRI CASTG 7 cut(s) 178, 406, 717, 1063, 1522, 1903, 2125
Tth111I GACNNNGTC 1 cut(s) 1959
Vha464I CTTAAG 1 cut(s) 2341
VpaK11BI GGWCC 6 cut(s) 499, 1454, 1582, 1872, 2216, 2271
XapI RAATTY 1 cut(s) 1095
XceI RCATGY 1 cut(s) 2291
XmiI GTMKAC 1 cut(s) 878
XmnI GAANNNNTTC 1 cut(s) 2169
XspI CTAG 3 cut(s) 1020, 1179, 1818
Zsp2I ATGCAT 1 cut(s) 1816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.