pycom02g06910

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0002261
Physical Location & Seq
Reverse (-)
4793058 .. 4795888
2831 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1776 bp
ATGACGAAAGCGGTGTTGCTGGGCGAAATAATCGATGGGGAAGAAGCAGATTTGCAGAGCTACAGTGCGGTGATGGTGGACGACGCACACGAGAGAGCGCTCTCGATTGACATTCTCTTCGGAGCTCTTAGGGAAATTACTCGAGCAAGACCGCATGGTTTTAAACTGCGTATCTCGTGTGCATCTCAAGTCGACGATTACTTGTTCTTGTACCATAAGTGGTACCATCGTAATAAAAAATTTCTGATTATTAGTCGACTTCATGGCTGCAAAAGTTTGAAGCTTGACGACTTTTCAGCTGACCCAGAAAAACGAGCGGAGAAGATGGAGAAGATGGAGAAGAAGCTTATGGATGCAGCCATGGCGGGAGATACGGGGGCGCTTAATGATCTGATCGCAGAGGACCCACTTGTTCTCGACAGAGCTTTAGTCTCCTGCGTCGCCGAGACGCCGCTTCACGTAGCTTCAATGCTGGGTCACCTGGCTTTTGTTACCGAGTTACTTAGTCGCAACCCCGAGTTGGCCTCCGAGCTGGACTCGCAGGGGTCAACGCCTCTCCACCTGGCGGCAGCGAAGGGGCACGTGGAGATAGTGAAAGAGCTGGTTCTGGCGGATCCTGGGGCGTGTGCGGTGAGGAACTTAGACGGGAGGACGGCGCTCCACGTCGCGGCTGCCAAGGGGCGGGTTAAGGTGGTGGGTGAGTTGGTCGGAGTTGGGAGTGAGTGGACTCGGGCGTTGACGGACAGAGGCGAGACGGCGCTGCACTTGTGCGTTGGGCGTAATCGGTTGGAGGGGTTGAAGGTTTTGGTTGAGGCAGTTGGGAATGATGATGAGTTTGTGAATTGGAAGGATTGTGATGGTAACACCGTTTTGCATATTGCCGTGGCCAAGAAGTTAATCGAGATTATCAAATTCTTGCTTTTTGAAACCGGAGTGGATGTGAATGCGTTGAATGCAAACAGCGCTACGGCTTTAGACATTCTGATGCAGAGTCCTAGAGATTTGAGGGATATGGAGATTGAGGATTTCCTTCGAGGTGCTGGGGCTCAGAGTGCAAGGGATATACGTAACATTGAGCACGACTGGGTTCGCGCTCCAACTAAAGAACGTCAGATACTAAGACATCGAGGCTTAGCACTGTCCTCGAAAGTGAGTGAGTCTATGAAACCGGGAGGGAACAAGAAGCCTACTGATTGGCTTGGTAGGAAGAGAAGTTCATTGATGGTGGTGGCATCTCTCCTTGCAACTGTTGCCTTCCAAGCTGTAATAACCCCTCCGGGAGGTGTTTGGCAGGATGACCTGCAAGTCGATGAAAATGGCAACCCTGTGGAGAATCCTCACACCGTAGGAACATCTGTCATGGCATATAAGAAGGGAAAAGAATATGGTCTAGTAATGATTTTTAACACAATCTCTTTCCTCGCATCCCTGAGCATAATTCTTCTGCTCGTTAGCGGGCTACCTATCAGACGGAGGAGATGGATGTGGATACAAATGGTCATAATGTGGGTTGCTGTCACAACTCTAGTAGCAACATATTTCATCTCTTTCCGTCAAATGTCACCTAATGACGAACATGTGCAGATCGTGTTACGTAAGGTAACCGAGATATCAGGTTGGACATGGTTGACTTTAATGGTGGTTGTTTTCTTTGGCAATGTTATTCGTATGAATCTATGGATTCTCAGAAAGTACGGTTATATTAAGCCAAAACATGTGGAGCCATCTAACGTTGATGATGAACTTGAACTCGGAGAGGTGAGAACCGAGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

592

Amino Acids

65.82

Weight (kDa)

6.31

Isoelectric Point (pI)

35.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 116 - 202 6.6e-13 Ankyrin repeats (3 copies)
Ank_4 PF13637 152 - 199 2.3e-06 Ankyrin repeats (many copies)
Ank_5 PF13857 174 - 223 3.3e-07 Ankyrin repeats (many copies)
Ank PF00023 182 - 208 2.2e-06 Ankyrin repeat
Ank_2 PF12796 246 - 316 1.6e-10 Ankyrin repeats (3 copies)
Ank_5 PF13857 280 - 327 1.4e-06 Ankyrin repeats (many copies)
Ank PF00023 286 - 318 5.6e-06 Ankyrin repeat
PGG PF13962 398 - 516 8.2e-24 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1304
Acc36I ACCTGC 1 cut(s) 1308
Acc65I GGTACC 1 cut(s) 222
AccB1I GGYRCC 1 cut(s) 222
AccBSI CCGCTC 1 cut(s) 317
AccI GTMKAC 2 cut(s) 192, 256
AccII CGCG 2 cut(s) 668, 1092
AclI AACGTT 1 cut(s) 1731
AclWI GGATC 2 cut(s) 608, 621
AcoI YGGCCR 1 cut(s) 885
AcsI RAATTY 2 cut(s) 239, 911
AcvI CACGTG 1 cut(s) 583
AcyI GRCGYC 1 cut(s) 449
AfaI GTAC 3 cut(s) 212, 224, 1694
AfeI AGCGCT 2 cut(s) 99, 964
AfiI CCNNNNNNNGG 5 cut(s) 520, 565, 667, 681, 1280
AflIII ACRYGT 2 cut(s) 1576, 1714
AgsI TTSAA 6 cut(s) 280, 468, 799, 926, 952, 1748
AjiI CACGTC 1 cut(s) 664
AjnI CCWGG 3 cut(s) 480, 561, 616
AloI GAACNNNNNNTCC 2 cut(s) 1198, 1230
Alw21I GWGCWC 2 cut(s) 127, 1080
Alw26I GTCTC 3 cut(s) 436, 440, 746
AlwI GGATC 2 cut(s) 608, 621
Ama87I CYCGRG 3 cut(s) 141, 515, 729
Aor51HI AGCGCT 2 cut(s) 99, 964
AoxI GGCC 2 cut(s) 522, 885
ApeKI GCWGC 5 cut(s) 267, 356, 569, 671, 760
ApoI RAATTY 2 cut(s) 239, 911
ArsI GACNNNNNNTTYG 2 cut(s) 101, 133
Asp718I GGTACC 1 cut(s) 222
AspLEI GCGC 6 cut(s) 100, 382, 658, 760, 965, 1094
AspS9I GGNCC 1 cut(s) 403
AsuC2I CCSGG 2 cut(s) 1170, 1278
AsuHPI GGTGA 6 cut(s) 82, 470, 643, 710, 1554, 1771
AvaI CYCGRG 3 cut(s) 141, 515, 729
AvaII GGWCC 1 cut(s) 403
BaeGI GKGCMC 1 cut(s) 582
BaeI ACNNNNGTAYC 2 cut(s) 214, 247
BalI TGGCCA 1 cut(s) 887
BamHI GGATCC 1 cut(s) 613
BanI GGYRCC 1 cut(s) 222
BanII GRGCYC 2 cut(s) 127, 1048
BauI CACGAG 2 cut(s) 89, 175
BbrPI CACGTG 1 cut(s) 583
Bbv12I GWGCWC 2 cut(s) 127, 1080
BbvI GCAGC 5 cut(s) 254, 368, 581, 658, 747
BccI CCATC 9 cut(s) 29, 67, 234, 319, 328, 851, 1216, 1473, 1732
BceAI ACGGC 4 cut(s) 669, 771, 866, 984
BcgI CGANNNNNNTGC 2 cut(s) 1125, 1159
BciT130I CCWGG 3 cut(s) 482, 563, 618
BciVI GTATCC 1 cut(s) 1482
BcnI CCSGG 2 cut(s) 1170, 1278
BcoDI GTCTC 3 cut(s) 436, 440, 746
BfaI CTAG 3 cut(s) 996, 1391, 1526
BfmI CTRYAG 1 cut(s) 61
BfoI RGCGCY 5 cut(s) 101, 383, 659, 761, 966
BfuAI ACCTGC 1 cut(s) 1308
BfuI GTATCC 1 cut(s) 1482
BisI GCNGC 8 cut(s) 268, 357, 452, 567, 570, 669, 672, 761
BlpI GCTNAGC 1 cut(s) 1132
BlsI GCNGC 8 cut(s) 269, 358, 453, 568, 571, 670, 673, 762
Bme1390I CCNGG 5 cut(s) 482, 563, 618, 1170, 1278
Bme18I GGWCC 1 cut(s) 403
BmeT110I CYCGRG 3 cut(s) 141, 515, 729
BmgBI CACGTC 1 cut(s) 664
BmgT120I GGNCC 1 cut(s) 403
BmiI GGNNCC 4 cut(s) 224, 405, 615, 1722
BmrFI CCNGG 5 cut(s) 482, 563, 618, 1170, 1278
BmrI ACTGGG 1 cut(s) 1093
BmsI GCATC 5 cut(s) 191, 343, 975, 1241, 1433
BmuI ACTGGG 1 cut(s) 1093
BplI GAGNNNNNCTC 4 cut(s) 509, 541, 521, 553
Bpu10I CCTNAGC 1 cut(s) 1430
Bpu1102I GCTNAGC 1 cut(s) 1132
BpuEI CTTGAG 1 cut(s) 171
BpuMI CCSGG 2 cut(s) 1170, 1278
Bsa29I ATCGAT 1 cut(s) 33
BsaAI YACGTR 4 cut(s) 460, 583, 1067, 1595
BsaHI GRCGYC 1 cut(s) 449
BsaJI CCNNGG 5 cut(s) 360, 617, 675, 882, 1767
BsaWI WCCGGW 1 cut(s) 929
BsaXI ACNNNNNCTCC 2 cut(s) 924, 954
Bsc4I CCNNNNNNNGG 5 cut(s) 520, 565, 667, 681, 1280
Bse1I ACTGG 1 cut(s) 1088
Bse3DI GCAATG 1 cut(s) 1663
BseBI CCWGG 3 cut(s) 482, 563, 618
BseCI ATCGAT 1 cut(s) 33
BseDI CCNNGG 5 cut(s) 360, 617, 675, 882, 1767
BseGI GGATG 5 cut(s) 358, 943, 1300, 1424, 1488
BseLI CCNNNNNNNGG 5 cut(s) 520, 565, 667, 681, 1280
BseMI GCAATG 1 cut(s) 1663
BseMII CTCAG 3 cut(s) 1061, 1421, 1699
BseNI ACTGG 1 cut(s) 1088
BseRI GAGGAG 1 cut(s) 1489
BseSI GKGCMC 1 cut(s) 582
BseXI GCAGC 5 cut(s) 254, 368, 581, 658, 747
BseYI CCCAGC 3 cut(s) 19, 472, 1040
BsgI GTGCAG 2 cut(s) 746, 1601
Bsh1236I CGCG 2 cut(s) 668, 1092
BshFI GGCC 2 cut(s) 524, 887
BshNI GGYRCC 1 cut(s) 222
BshVI ATCGAT 1 cut(s) 33
BsiHKAI GWGCWC 2 cut(s) 127, 1080
BsiHKCI CYCGRG 3 cut(s) 141, 515, 729
BsiSI CCGG 3 cut(s) 930, 1169, 1277
BslI CCNNNNNNNGG 5 cut(s) 520, 565, 667, 681, 1280
BsmAI GTCTC 3 cut(s) 436, 440, 746
BsmBI CGTCTC 2 cut(s) 440, 746
BsmI GAATGC 2 cut(s) 949, 958
BsnI GGCC 2 cut(s) 524, 887
BsoBI CYCGRG 3 cut(s) 141, 515, 729
Bsp1286I GDGCHC 4 cut(s) 127, 582, 1048, 1080
Bsp143I GATC 4 cut(s) 388, 393, 613, 1584
Bsp1720I GCTNAGC 1 cut(s) 1132
Bsp19I CCATGG 1 cut(s) 360
BspANI GGCC 2 cut(s) 524, 887
BspCNI CTCAG 3 cut(s) 1060, 1422, 1698
BspDI ATCGAT 1 cut(s) 33
BspFNI CGCG 2 cut(s) 668, 1092
BspLI GGNNCC 4 cut(s) 224, 405, 615, 1722
BspMI ACCTGC 1 cut(s) 1308
BspPI GGATC 2 cut(s) 608, 621
BspT107I GGYRCC 1 cut(s) 222
BsrBI CCGCTC 1 cut(s) 317
BsrDI GCAATG 1 cut(s) 1663
BsrI ACTGG 1 cut(s) 1088
BssECI CCNNGG 5 cut(s) 360, 617, 675, 882, 1767
BssMI GATC 4 cut(s) 388, 393, 613, 1584
BssNI GRCGYC 1 cut(s) 449
BssSI CACGAG 2 cut(s) 89, 175
BssT1I CCWWGG 2 cut(s) 360, 675
Bst2BI CACGAG 2 cut(s) 89, 175
Bst2UI CCWGG 3 cut(s) 482, 563, 618
Bst4CI ACNGT 6 cut(s) 65, 868, 1140, 1249, 1345, 1697
Bst6I CTCTTC 2 cut(s) 122, 1202
BstACI GRCGYC 1 cut(s) 449
BstAPI GCANNNNNTGC 1 cut(s) 1250
BstBAI YACGTR 4 cut(s) 460, 583, 1067, 1595
BstC8I GCNNGC 1 cut(s) 1457
BstDEI CTNAG 8 cut(s) 128, 503, 640, 1047, 1118, 1132, 1430, 1685
BstDSI CCRYGG 2 cut(s) 360, 882
BstEII GGTNACC 2 cut(s) 476, 1600
BstENI CCTNNNNNAGG 1 cut(s) 1278
BstF5I GGATG 5 cut(s) 358, 943, 1300, 1424, 1488
BstFNI CGCG 2 cut(s) 668, 1092
BstH2I RGCGCY 5 cut(s) 101, 383, 659, 761, 966
BstHHI GCGC 6 cut(s) 100, 382, 658, 760, 965, 1094
BstKTI GATC 4 cut(s) 391, 396, 616, 1587
BstMAI GTCTC 3 cut(s) 436, 440, 746
BstMBI GATC 4 cut(s) 388, 393, 613, 1584
BstMWI GCNNNNNNNGC 7 cut(s) 362, 538, 953, 962, 1052, 1250, 1259
BstNI CCWGG 3 cut(s) 482, 563, 618
BstNSI RCATGY 2 cut(s) 1580, 1718
BstPI GGTNACC 2 cut(s) 476, 1600
BstSCI CCNGG 5 cut(s) 480, 561, 616, 1168, 1276
BstSFI CTRYAG 1 cut(s) 61
BstSLI GKGCMC 1 cut(s) 582
BstSNI TACGTA 2 cut(s) 1067, 1595
BstUI CGCG 2 cut(s) 668, 1092
BstV1I GCAGC 5 cut(s) 254, 368, 581, 658, 747
BstX2I RGATCY 1 cut(s) 613
BstYI RGATCY 1 cut(s) 613
Bsu15I ATCGAT 1 cut(s) 33
BsuI GTATCC 1 cut(s) 1482
BsuRI GGCC 2 cut(s) 524, 887
BsuTUI ATCGAT 1 cut(s) 33
BtgI CCRYGG 2 cut(s) 360, 882
BtrI CACGTC 1 cut(s) 664
BtsCI GGATG 5 cut(s) 358, 943, 1300, 1424, 1488
BtsIMutI CAGTG 2 cut(s) 70, 1136
BveI ACCTGC 1 cut(s) 1308
Cac8I GCNNGC 1 cut(s) 1457
CfoI GCGC 6 cut(s) 100, 382, 658, 760, 965, 1094
Cfr13I GGNCC 1 cut(s) 403
ClaI ATCGAT 1 cut(s) 33
CseI GACGC 3 cut(s) 92, 427, 457
Csp6I GTAC 3 cut(s) 211, 223, 1693
CspCI CAANNNNNGTGG 2 cut(s) 1698, 1733
CviAII CATG 7 cut(s) 155, 263, 361, 1360, 1577, 1623, 1715
CviQI GTAC 3 cut(s) 211, 223, 1693
DdeI CTNAG 8 cut(s) 128, 503, 640, 1047, 1118, 1132, 1430, 1685
DpnI GATC 4 cut(s) 390, 395, 615, 1586
DpnII GATC 4 cut(s) 388, 393, 613, 1584
DraI TTTAAA 1 cut(s) 163
DrdI GACNNNNNNGTC 1 cut(s) 1304
DseDI GACNNNNNNGTC 1 cut(s) 1304
EaeI YGGCCR 1 cut(s) 885
Eam1104I CTCTTC 2 cut(s) 122, 1202
EarI CTCTTC 2 cut(s) 122, 1202
EciI GGCGGA 1 cut(s) 626
Ecl136II GAGCTC 1 cut(s) 125
Eco105I TACGTA 2 cut(s) 1067, 1595
Eco130I CCWWGG 2 cut(s) 360, 675
Eco24I GRGCYC 2 cut(s) 127, 1048
Eco32I GATATC 1 cut(s) 1611
Eco47I GGWCC 1 cut(s) 403
Eco47III AGCGCT 2 cut(s) 99, 964
Eco53kI GAGCTC 1 cut(s) 125
Eco72I CACGTG 1 cut(s) 583
Eco88I CYCGRG 3 cut(s) 141, 515, 729
Eco91I GGTNACC 2 cut(s) 476, 1600
EcoICRI GAGCTC 1 cut(s) 125
EcoNI CCTNNNNNAGG 1 cut(s) 1278
EcoO109I RGGNCCY 1 cut(s) 403
EcoO65I GGTNACC 2 cut(s) 476, 1600
EcoRII CCWGG 3 cut(s) 480, 561, 616
EcoRV GATATC 1 cut(s) 1611
EcoT14I CCWWGG 2 cut(s) 360, 675
EcoT38I GRGCYC 2 cut(s) 127, 1048
ErhI CCWWGG 2 cut(s) 360, 675
Esp3I CGTCTC 2 cut(s) 440, 746
FaeI CATG 7 cut(s) 158, 266, 364, 1363, 1580, 1626, 1718
FatI CATG 7 cut(s) 154, 262, 360, 1359, 1576, 1622, 1714
FauI CCCGC 3 cut(s) 358, 675, 1448
FblI GTMKAC 2 cut(s) 192, 256
Fnu4HI GCNGC 8 cut(s) 268, 357, 452, 567, 570, 669, 672, 761
FokI GGATG 5 cut(s) 365, 950, 1307, 1411, 1495
FriOI GRGCYC 2 cut(s) 127, 1048
Fsp4HI GCNGC 8 cut(s) 268, 357, 452, 567, 570, 669, 672, 761
FspBI CTAG 3 cut(s) 996, 1391, 1526
GlaI GCGC 6 cut(s) 99, 381, 657, 759, 964, 1093
GluI GCNGC 8 cut(s) 268, 357, 452, 567, 570, 669, 672, 761
GsaI CCCAGC 3 cut(s) 23, 476, 1044
HaeII RGCGCY 5 cut(s) 101, 383, 659, 761, 966
HaeIII GGCC 2 cut(s) 524, 887
HapII CCGG 3 cut(s) 930, 1169, 1277
HgaI GACGC 3 cut(s) 92, 427, 457
HhaI GCGC 6 cut(s) 100, 382, 658, 760, 965, 1094
Hin1I GRCGYC 1 cut(s) 449
Hin1II CATG 7 cut(s) 158, 266, 364, 1363, 1580, 1626, 1718
Hin6I GCGC 6 cut(s) 98, 380, 656, 758, 963, 1092
HinP1I GCGC 6 cut(s) 98, 380, 656, 758, 963, 1092
HincII GTYRAC 5 cut(s) 193, 257, 549, 738, 1629
HindII GTYRAC 5 cut(s) 193, 257, 549, 738, 1629
HindIII AAGCTT 2 cut(s) 281, 344
HinfI GANTC 7 cut(s) 536, 727, 991, 1157, 1333, 1672, 1681
HpaII CCGG 3 cut(s) 930, 1169, 1277
HphI GGTGA 6 cut(s) 82, 470, 643, 710, 1554, 1771
Hpy166II GTNNAC 7 cut(s) 79, 193, 257, 549, 726, 738, 1629
Hpy188III TCNNGA 3 cut(s) 103, 416, 901
Hpy8I GTNNAC 7 cut(s) 79, 193, 257, 549, 726, 738, 1629
Hpy99I CGWCG 4 cut(s) 86, 197, 443, 668
HpyAV CCTTC 6 cut(s) 568, 793, 841, 1040, 1264, 1366
HpyCH4III ACNGT 6 cut(s) 65, 868, 1140, 1249, 1345, 1697
HpyCH4IV ACGT 7 cut(s) 459, 582, 663, 1066, 1108, 1594, 1731
HpyF10VI GCNNNNNNNGC 7 cut(s) 362, 538, 953, 962, 1052, 1250, 1259
HpyF3I CTNAG 8 cut(s) 128, 503, 640, 1047, 1118, 1132, 1430, 1685
HpySE526I ACGT 7 cut(s) 459, 582, 663, 1066, 1108, 1594, 1731
Hsp92I GRCGYC 1 cut(s) 449
Hsp92II CATG 7 cut(s) 158, 266, 364, 1363, 1580, 1626, 1718
HspAI GCGC 6 cut(s) 98, 380, 656, 758, 963, 1092
KpnI GGTACC 1 cut(s) 226
Kzo9I GATC 4 cut(s) 388, 393, 613, 1584
LmnI GCTCC 4 cut(s) 122, 663, 1099, 1720
Lsp1109I GCAGC 5 cut(s) 254, 368, 581, 658, 747
LweI GCATC 5 cut(s) 191, 343, 975, 1241, 1433
MaeI CTAG 3 cut(s) 996, 1391, 1526
MaeII ACGT 7 cut(s) 459, 582, 663, 1066, 1108, 1594, 1731
MaeIII GTNAC 9 cut(s) 476, 490, 498, 860, 1067, 1516, 1560, 1590, 1600
MalI GATC 4 cut(s) 390, 395, 615, 1586
MbiI CCGCTC 1 cut(s) 317
MboI GATC 4 cut(s) 388, 393, 613, 1584
MboII GAAGA 7 cut(s) 53, 109, 334, 343, 352, 1219, 1433
MflI RGATCY 1 cut(s) 613
MhlI GDGCHC 4 cut(s) 127, 582, 1048, 1080
MlsI TGGCCA 1 cut(s) 887
MluCI AATT 5 cut(s) 135, 239, 841, 911, 1437
MluNI TGGCCA 1 cut(s) 887
MlyI GAGTC 4 cut(s) 530, 721, 1000, 1166
MmeI TCCRAC 4 cut(s) 688, 768, 1121, 1598
Mox20I TGGCCA 1 cut(s) 887
MscI TGGCCA 1 cut(s) 887
MseI TTAA 8 cut(s) 162, 384, 687, 896, 1404, 1634, 1704, 1774
MslI CAYNNNNRTG 1 cut(s) 983
Msp20I TGGCCA 1 cut(s) 887
MspA1I CMGCKG 1 cut(s) 299
MspI CCGG 3 cut(s) 930, 1169, 1277
MspR9I CCNGG 5 cut(s) 482, 563, 618, 1170, 1278
Mva1269I GAATGC 2 cut(s) 949, 958
MvaI CCWGG 3 cut(s) 482, 563, 618
MvnI CGCG 2 cut(s) 668, 1092
MwoI GCNNNNNNNGC 7 cut(s) 362, 538, 953, 962, 1052, 1250, 1259
NciI CCSGG 2 cut(s) 1170, 1278
NcoI CCATGG 1 cut(s) 360
NdeII GATC 4 cut(s) 388, 393, 613, 1584
NlaIII CATG 7 cut(s) 158, 266, 364, 1363, 1580, 1626, 1718
NlaIV GGNNCC 4 cut(s) 224, 405, 615, 1722
NmeAIII GCCGAG 1 cut(s) 469
NmuCI GTSAC 3 cut(s) 476, 1516, 1560
NspI RCATGY 2 cut(s) 1580, 1718
PaeR7I CTCGAG 1 cut(s) 141
PciI ACATGT 2 cut(s) 1576, 1714
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PctI GAATGC 2 cut(s) 949, 958
PfeI GAWTC 3 cut(s) 1333, 1672, 1681
PfoI TCCNGGA 1 cut(s) 1276
PkrI GCNGC 8 cut(s) 269, 358, 453, 568, 571, 670, 673, 762
PleI GAGTC 4 cut(s) 530, 721, 999, 1165
PmaCI CACGTG 1 cut(s) 583
PmlI CACGTG 1 cut(s) 583
PpsI GAGTC 4 cut(s) 530, 721, 999, 1165
Ppu21I YACGTR 4 cut(s) 460, 583, 1067, 1595
PpuMI RGGWCCY 1 cut(s) 403
PscI ACATGT 2 cut(s) 1576, 1714
Psp124BI GAGCTC 1 cut(s) 127
Psp1406I AACGTT 1 cut(s) 1731
Psp5II RGGWCCY 1 cut(s) 403
Psp6I CCWGG 3 cut(s) 480, 561, 616
PspCI CACGTG 1 cut(s) 583
PspEI GGTNACC 2 cut(s) 476, 1600
PspFI CCCAGC 3 cut(s) 19, 472, 1040
PspGI CCWGG 3 cut(s) 480, 561, 616
PspN4I GGNNCC 4 cut(s) 224, 405, 615, 1722
PspPI GGNCC 1 cut(s) 403
PspPPI RGGWCCY 1 cut(s) 403
PspXI VCTCGAGB 1 cut(s) 141
PsrI GAACNNNNNNTAC 2 cut(s) 1098, 1130
PsuI RGATCY 1 cut(s) 613
PvuII CAGCTG 1 cut(s) 299
RsaI GTAC 3 cut(s) 212, 224, 1694
RsaNI GTAC 3 cut(s) 211, 223, 1693
RseI CAYNNNNRTG 1 cut(s) 983
SacI GAGCTC 1 cut(s) 127
SalI GTCGAC 2 cut(s) 191, 255
SaqAI TTAA 8 cut(s) 162, 384, 687, 896, 1404, 1634, 1704, 1774
SatI GCNGC 8 cut(s) 268, 357, 452, 567, 570, 669, 672, 761
Sau3AI GATC 4 cut(s) 388, 393, 613, 1584
Sau96I GGNCC 1 cut(s) 403
SchI GAGTC 4 cut(s) 530, 721, 1000, 1166
ScrFI CCNGG 5 cut(s) 482, 563, 618, 1170, 1278
SduI GDGCHC 4 cut(s) 127, 582, 1048, 1080
SfaNI GCATC 5 cut(s) 191, 343, 975, 1241, 1433
SfcI CTRYAG 1 cut(s) 61
Sfr274I CTCGAG 1 cut(s) 141
SinI GGWCC 1 cut(s) 403
SlaI CTCGAG 1 cut(s) 141
SmiMI CAYNNNNRTG 1 cut(s) 983
SmlI CTYRAG 2 cut(s) 141, 186
SmoI CTYRAG 2 cut(s) 141, 186
SnaBI TACGTA 2 cut(s) 1067, 1595
Sse9I AATT 5 cut(s) 135, 239, 841, 911, 1437
SspMI CTAG 3 cut(s) 996, 1391, 1526
SstI GAGCTC 1 cut(s) 127
StyD4I CCNGG 5 cut(s) 480, 561, 616, 1168, 1276
StyI CCWWGG 2 cut(s) 360, 675
TaaI ACNGT 6 cut(s) 65, 868, 1140, 1249, 1345, 1697
TaiI ACGT 7 cut(s) 462, 585, 666, 1069, 1111, 1597, 1734
TasI AATT 5 cut(s) 135, 239, 841, 911, 1437
TauI GCSGC 3 cut(s) 454, 569, 671
TfiI GAWTC 3 cut(s) 1333, 1672, 1681
Tru1I TTAA 8 cut(s) 162, 384, 687, 896, 1404, 1634, 1704, 1774
Tru9I TTAA 8 cut(s) 162, 384, 687, 896, 1404, 1634, 1704, 1774
TscAI CASTG 2 cut(s) 70, 1143
TseFI GTSAC 3 cut(s) 476, 1516, 1560
TseI GCWGC 5 cut(s) 267, 356, 569, 671, 760
Tsp45I GTSAC 3 cut(s) 476, 1516, 1560
TspDTI ATGAA 7 cut(s) 251, 1178, 1206, 1326, 1531, 1685, 1755
TspGWI ACGGA 3 cut(s) 755, 1486, 1541
TspRI CASTG 2 cut(s) 70, 1143
VpaK11BI GGWCC 1 cut(s) 403
XagI CCTNNNNNAGG 1 cut(s) 1278
XapI RAATTY 2 cut(s) 239, 911
XceI RCATGY 2 cut(s) 1580, 1718
XhoI CTCGAG 1 cut(s) 141
XmiI GTMKAC 2 cut(s) 192, 256
XspI CTAG 3 cut(s) 996, 1391, 1526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.