Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr2

Orthogroup ID

OG0005903

Physical Location & Seq

Genomic Coordinates Forward (+)

16705680 .. 16710962

Gene Structure 5283 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3843 bp

ATGAACCCACCATATAATATGTTTACTTTGTCGAACTCCAATATTATCACCATGGTTCTCTTAAAAAATCTTCCTAAGAAGACCCAAGGTCGAAAGAGGATCGAGATCAAGAAACTTGAGAACCTTAACAGCAAACAAGTCACCTTCTCCAAGCGCCGCGCTGGTATATTCAAGAAGGCTGCGGAACTGAGCGTTTTGTGCGGCGCTGATGTGGCGGTCATTGTTTTCTCGACAGCAGGAAAAGTGTTTTGCTATGGGCACCCTAGCGTGGACACCACTCTAGATTGGTATTGCAGTGGGGTAACTCCTCCTGCAGTGTCTGATGATACCGCAGGAGATCAAAGCCCTAATAGGGTTCCCGTGGCAGAGTTCAACAAGGAGTACATGGAGGCTATTAGGGAGTTGGAGGAACAGAAGAAGAGAGTTGCGGAGGTCAAGGAGGCGGCAAAGATGAAGAAGGTTGTGATGGGTGTAGTGGGTGGGTGTGGTGAGAGGTTTTGGTGGGAGGATGAGAGTGTTGATTTGGATGCGTTGGATGTGCAGGAAGTCGAGCATTACTTGATCACATTAGAGGATGTGAGGAAGCAGGTTGCCGCTAGGCTTCATGAGATGAAGATTTTGAATGGGACGTTGAACCCGGGGGCGCTTCCACCGATTCCGGCAGTTCATCGACTTTGTCATCTTCCAATGATGACAAATGGTTTTAGTGGTCACGGTGCCCAATTTGGTGGTGTATTTCTTTTTTCTTTTTCTCATTTTTTGTTAATCATGTTGTTATGTTTGTGCAATAAAATGTTTATGCTCCTTTCTTTTGATAGAGCAATGCCACCTTGTGCTATATTCATAAAAATTAATTCTTGCAGAACAATGAAGCAAAACACTTACCTTTTCAGATACGTAACCGAAACCAAGTTCTGTAGGTTTGATGTCAAATTAGGGTTTGGGGAAGTTCTTACTGCAGAAGTTTGGGGACTTTATTATGGTGTTTCTGTTGCTTCGCGAATGGGGTTTCACCATGTGCTTGTGGACGTGTGGTTTCTGCTATTGCTGTTCGGCTTGTCAATTTGTTCGACTCTAATTTTTTTTTTTTTTAGTTGTCTAGTTTTTCATGCCACATTGAGGTCTGATTACAACTCGAGACCAACCCATGTTACACTTGTATATAGCACCTTCTTGCATATGGTGTTCGCTTGCAACCACACAATTAAAGTAGTGAGAAAGATAATAATTCCAGCTATATATATGGTTCTAAACGTACCCGAACTTACTCCAACTCTTGAACCATATATGAGCTTTAACCCAAAACTCATTTTTGGAATGTGTGTTTTTTTAAATTTAAAAAAAAATTCTTCCATGTGTTTTATATTCTTTAATAATTTAGGGACTCCAATCACCACTTTAGAGTATTCATTTAAGGACTTAAAGGGAATCTTTGTTAGAATCTTTAAATTTGAAGTTTTTATGCTTTTATGTGATAATTTGATTAGTAAAAGCATTAAAATCTTCAATCAAGATTGTGGATCCCGTTTTCCACTAAATATTGAAAGCATTGAAATTAAAAACATTAAAAGCATTAAAAGCTTCAATCAAGATTGTGGCAACTTTTTTCCACTCAAGGTGTGTGAAATGACTAAAAGGCCCTTGGAGGCGAGAGTGTTGAATTTCGTTGATTCTAGTGGTATTCATCACTACGAAAGCGTGGGCACAAGCAGAATCAATTTCGGAGTTATGATGATCGTTTTAGGGAATTACGAAGTTGGGGGGCAAAATAGTCAATTCATTATTCAAGATATTAGGGTTGGATATTTGTGTTATTTTAATAATTTTCTGGTGAGTTTTTTTGGCCAATGGGGTTCACACCCCACTTCTCCTCCTTCCCCGTTTCCCTTGTCTCTCTTTCCCTCATACTCTTTTTCATCACTCTCTCCCTCCTCTGTAAAACCCAAGAGGCACCTCCACTGGCCTCGCCACTCTGGTGAGTACCCCCACCACCAGCTAATCTTCTTCATCACGAACCCAAGCCAAGGCCTTGAACCACACAGCGGTAAAGACCACTATTCACTTTCTCCTTCGTCAAACTCCATGCGAGTTGTCGACAATTCCGAAGAAGACCAATATGGGGGAAGCTCTCCCCTATTTTTTGGCGAGAACAACAATTTTTGCAGGCGGTTTTTGGCCACGTCCGGCCACCATTTGGCTTCCAAATTGGAGCGTTTGTTAGTAGCGACCCCATCCTCCCTAATAAATACTAGGATTGCATTCATGAGAAGCTTTAAGTTAGAAATTTTCTATGATTTGCGATCTGAGATTTCAATTGAGCTAGAACGCTCCTATGGATTTATTGTAAAACTCATTAGGATTAGAATTCATTTGTTAAACTCTCATGTCTGTTTAGTGTTAGTACAAGAATTCTACCTGAAGAAGACATCTAGTTACTTGCACAAGGATCGCTCTTCTACTCAATGGAAAACCTTATGGTTCGCTCTAGTAATGGATCTAGAATACTTAATGGAATGCACACTTTCCATGATCAAGGACCAATATATTTATAGAAATCTCAAGAACTTCTGCTTAATGGCAATCGTGGGTTGCTCCACTATCTCAATGTGTGTATCTTATGGTCCTACTTTCAAGGAATATTCCAAACTAAGTTCTTACATTCTCCCACTTTGGACTTTACAAATCATGTTTAATCATCAATATATGTTTCAACAACGCCAGTTCCTTAGTGATCACATGGGTCAATATGTTCGAACCCTAGAAAATCTTGTGCAAAATTTAGCACAAGCACTTCGAAATTACATCCCTCTTTCATCAGTCACACATGATACATATACACCCTTATGCCCTTTAATTAAGTTATCATACTTTAACTTAATCACCTTTTATGAGTTTATTGCCTTGGTTTACAGTACATGTTTGCCTATATGCATAACTTTCATGCAATATTTCAGTCTGAATGAAATATACAACAACACACAATCATGTGCTGAAAATTTGAAACATACTAACTTTTACTCTCACGCTTCAGTAGAATCAAATGACTCTAGCACTCCCATCTTTGTGACATGCTACTTGAAAATTCCCACTGCAAGAGGCTTTGTGAGAGGATATGCAATCATTGATGCAAAATCAATGTGTTCTATTGAAAACATTCCTTTCTTGACATTTTCCTTCATGGCCAGAAACTTCACATCCATAAGTCTTGTAGTTGAGGACTTCTTGACCAATGGTCTAGGGATTGAATCCACCACCATTAAAAATGAAATGATATTTTTTAGCAATAGCCCTTGTTCCATGACTTCGAAACAAGCAATGTACTCAGCTTGCATTGTGGATGTTGCTAGGGTTTTCTGTTTGACCTTCGATCATCCACACACCTTGCAAAATCAGCACCTGTATACCCTAGTAGCTCCAAATTATCAACCCTTTTATAGACTACTGTTTTCCAGTGTTGTTCTCCAAGATTGGCTAGAGAAGCATATGGCTTTCTTGTTGAGTTTGTCTTTCTTTCCCATAGAGACTTCACTATTTAAACAATTATATAAACCTAAAAGATTTCTCTTTCTGTCTTGTATGATCTCTATTCCCAAAACAAAATATGCTTCTCCAAGATCTTTCATATCAAATGTTTTGTCCAGCACCCTTTTTATTTCCAGCAACAAGTTCAAATCATTGCTAGCCAAGAGTATGTCATCAACACAAAGCACAAGGAAAATGAACTTCGAACCACAAACCTTAATGTACAAGCAGTCTTCAATTTTGTTTTCTGTAAAACCCAAAGTTGTGATTTTCTCTTCAAATTTAAGGAACCATTACCTTGATGCTTGTTTCAATCCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0009791 GO:0009888 GO:0009889 GO:0009960 GO:0010154 GO:0010468 GO:0010556 GO:0010817 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032879 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051049 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0065008 GO:0080090 GO:0140110 GO:1903506 GO:2000012 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04010 ko04011 ko04013 ko04022 ko04111 ko04371 ko04921 ko05202 ko05418 map04010 map04011 map04013 map04022 map04111 map04371 map04921 map05202 map05418

Pfam Domains

Protein Families

SRF-TF

Protein Analysis

1281

Amino Acids

146.69

Weight (kDa)

8.98

Isoelectric Point (pI)

41.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SRF-TF	PF00319	38 - 85	3.6e-22	SRF-type transcription factor (DNA-binding and dimerisation domain)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005903)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g40280 FvH4_4g35860
malus_domestica	MD02G1221000.v1.1 MD15G1322300.v1.1
prunus_persica	Prupe.2G120200_v2.0.a1
pyrus_communis	pycom02g18540
rosa_chinensis	RchiOBHm_Chr3g0480601 RchiOBHm_Chr4g0445441 RchiOBHm_Chr6g0300471
rosa_laevigata	RLG00000005740 RLG00000011335 RLG00000012216
rosa_multiflora	Rmu_sc0001476.1_g000003 Rmu_sc0001509.1_g000008
rosa_roxburghii	Rroxscaffold_5G00385950 Rroxscaffold_7G00167760
rosa_rugosa	Rorug04G0361300 Rorug06G0296300
rosa_samantha	Rh4AG422800 Rh4BG434800 Rh4CG449700 Rh4DG431000 Rh6AG408900 Rh6BG416200 Rh6CG423700 Rh6DG409400
rosa_wichuraiana	Rw4G036260 Rw6G035780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	577
AccB1I	GGYRCC	3 cut(s)	258, 716, 1950
AccB7I	CCANNNNNTGG	1 cut(s)	2194
AccI	GTMKAC	2 cut(s)	2094, 3401
AccII	CGCG	2 cut(s)	159, 1000
AclWI	GGATC	5 cut(s)	107, 1515, 1528, 2454, 2502
AcoI	YGGCCR	4 cut(s)	1843, 2175, 2185, 3180
AcuI	CTGAAG	2 cut(s)	2438, 3012
AdeI	CACNNNGTG	2 cut(s)	833, 1018
AfaI	GTAC	7 cut(s)	383, 1257, 1982, 2403, 2914, 3320, 3746
AfiI	CCNNNNNNNGG	7 cut(s)	268, 352, 1119, 2024, 2042, 2119, 2194
AflIII	ACRYGT	2 cut(s)	1029, 2915
AhdI	GACNNNNNGTC	2 cut(s)	87, 3231
AjiI	CACGTC	2 cut(s)	1030, 2181
AleI	CACNNNNGTG	1 cut(s)	1974
AluBI	AGCT	9 cut(s)	1235, 1293, 1581, 1996, 2127, 2271, 2320, 3326, 3413
AluI	AGCT	9 cut(s)	1235, 1293, 1581, 1996, 2127, 2271, 2320, 3326, 3413
Alw26I	GTCTC	3 cut(s)	1132, 1896, 3515
AlwI	GGATC	5 cut(s)	107, 1515, 1528, 2454, 2502
AlwNI	CAGNNNCTG	2 cut(s)	320, 3397
Ama87I	CYCGRG	2 cut(s)	637, 1135
AoxI	GGCC	7 cut(s)	1637, 1843, 1961, 2026, 2175, 2185, 3180
ApeKI	GCWGC	1 cut(s)	179
ArsI	GACNNNNNNTTYG	2 cut(s)	3620, 3652
AseI	ATTAAT	1 cut(s)	852
Asp700I	GAANNNNTTC	1 cut(s)	3153
AspLEI	GCGC	4 cut(s)	156, 161, 206, 646
AspS9I	GGNCC	3 cut(s)	1638, 2536, 2621
AsuC2I	CCSGG	2 cut(s)	638, 639
AsuHPI	GGTGA	8 cut(s)	40, 133, 500, 1004, 1384, 1843, 1988, 2872
AsuII	TTCGAA	4 cut(s)	2752, 2794, 3305, 3726
AsuNHI	GCTAGC	1 cut(s)	3679
AvaI	CYCGRG	2 cut(s)	637, 1135
AvaII	GGWCC	2 cut(s)	2536, 2621
BaeGI	GKGCMC	3 cut(s)	261, 721, 1706
BalI	TGGCCA	3 cut(s)	1845, 2177, 3182
BamHI	GGATCC	1 cut(s)	1520
BanI	GGYRCC	3 cut(s)	258, 716, 1950
BbsI	GAAGAC	4 cut(s)	86, 2115, 2429, 3747
BbvI	GCAGC	1 cut(s)	166
BccI	CCATC	3 cut(s)	460, 2239, 3065
BcgI	CGANNNNNNTGC	2 cut(s)	2429, 2463
BclI	TGATCA	3 cut(s)	561, 2529, 2731
BcnI	CCSGG	2 cut(s)	638, 639
BcoDI	GTCTC	3 cut(s)	1132, 1896, 3515
BfmI	CTRYAG	3 cut(s)	312, 916, 957
BfoI	RGCGCY	3 cut(s)	157, 207, 647
BfuAI	ACCTGC	1 cut(s)	577
BglII	AGATCT	1 cut(s)	3614
BisI	GCNGC	5 cut(s)	157, 180, 202, 444, 594
BlsI	GCNGC	5 cut(s)	158, 181, 203, 445, 595
Bme1390I	CCNGG	2 cut(s)	638, 639
Bme18I	GGWCC	2 cut(s)	2536, 2621
BmeRI	GACNNNNNGTC	2 cut(s)	87, 3231
BmeT110I	CYCGRG	2 cut(s)	637, 1135
BmgBI	CACGTC	2 cut(s)	1030, 2181
BmgT120I	GGNCC	3 cut(s)	1638, 2536, 2621
BmiI	GGNNCC	6 cut(s)	260, 357, 718, 1522, 1952, 3812
BmrFI	CCNGG	2 cut(s)	638, 639
BmsI	GCATC	3 cut(s)	517, 3115, 3814
BmtI	GCTAGC	1 cut(s)	3683
BpiI	GAAGAC	4 cut(s)	86, 2115, 2429, 3747
Bpu14I	TTCGAA	4 cut(s)	2752, 2794, 3305, 3726
BpuEI	CTTGAG	3 cut(s)	137, 1598, 2543
BpuMI	CCSGG	2 cut(s)	638, 639
BsaAI	YACGTR	1 cut(s)	898
BsaBI	GATNNNNATC	1 cut(s)	104
BsaI	GGTCTC	1 cut(s)	1132
BsaJI	CCNNGG	8 cut(s)	51, 85, 360, 637, 638, 1641, 2023, 2901
BsaXI	ACNNNNNCTCC	4 cut(s)	1855, 1885, 2201, 2231
Bsc4I	CCNNNNNNNGG	7 cut(s)	268, 352, 1119, 2024, 2042, 2119, 2194
Bse1I	ACTGG	3 cut(s)	1964, 2719, 3450
Bse3DI	GCAATG	3 cut(s)	828, 3321, 3674
Bse8I	GATNNNNATC	1 cut(s)	104
BseDI	CCNNGG	8 cut(s)	51, 85, 360, 637, 638, 1641, 2023, 2901
BseGI	GGATG	9 cut(s)	514, 532, 541, 580, 2231, 2802, 3194, 3343, 3370
BseJI	GATNNNNATC	1 cut(s)	104
BseLI	CCNNNNNNNGG	7 cut(s)	268, 352, 1119, 2024, 2042, 2119, 2194
BseMI	GCAATG	3 cut(s)	828, 3321, 3674
BseMII	CTCAG	3 cut(s)	179, 2295, 3336
BseNI	ACTGG	3 cut(s)	1964, 2719, 3450
BseRI	GAGGAG	3 cut(s)	297, 1860, 1921
BseSI	GKGCMC	3 cut(s)	261, 721, 1706
BseXI	GCAGC	1 cut(s)	166
BsgI	GTGCAG	1 cut(s)	560
Bsh1236I	CGCG	2 cut(s)	159, 1000
BshFI	GGCC	7 cut(s)	1639, 1845, 1963, 2028, 2177, 2187, 3182
BshNI	GGYRCC	3 cut(s)	258, 716, 1950
BsiHKCI	CYCGRG	2 cut(s)	637, 1135
BsiSI	CCGG	3 cut(s)	638, 659, 2184
BslFI	GGGAC	3 cut(s)	640, 984, 1396
BslI	CCNNNNNNNGG	7 cut(s)	268, 352, 1119, 2024, 2042, 2119, 2194
BsmAI	GTCTC	3 cut(s)	1132, 1896, 3515
BsmFI	GGGAC	3 cut(s)	640, 984, 1396
BsmI	GAATGC	2 cut(s)	2258, 2519
BsnI	GGCC	7 cut(s)	1639, 1845, 1963, 2028, 2177, 2187, 3182
Bso31I	GGTCTC	1 cut(s)	1132
BsoBI	CYCGRG	2 cut(s)	637, 1135
Bsp119I	TTCGAA	4 cut(s)	2752, 2794, 3305, 3726
Bsp1286I	GDGCHC	3 cut(s)	261, 721, 1706
Bsp1407I	TGTACA	1 cut(s)	3744
Bsp19I	CCATGG	1 cut(s)	51
Bsp68I	TCGCGA	1 cut(s)	1000
BspANI	GGCC	7 cut(s)	1639, 1845, 1963, 2028, 2177, 2187, 3182
BspCNI	CTCAG	3 cut(s)	180, 2296, 3335
BspFNI	CGCG	2 cut(s)	159, 1000
BspHI	TCATGA	2 cut(s)	604, 2262
BspLI	GGNNCC	6 cut(s)	260, 357, 718, 1522, 1952, 3812
BspMAI	CTGCAG	2 cut(s)	316, 961
BspMI	ACCTGC	1 cut(s)	577
BspOI	GCTAGC	1 cut(s)	3683
BspPI	GGATC	5 cut(s)	107, 1515, 1528, 2454, 2502
BspQI	GCTCTTC	1 cut(s)	2458
BspT104I	TTCGAA	4 cut(s)	2752, 2794, 3305, 3726
BspT107I	GGYRCC	3 cut(s)	258, 716, 1950
BspTNI	GGTCTC	1 cut(s)	1132
BsrDI	GCAATG	3 cut(s)	828, 3321, 3674
BsrGI	TGTACA	1 cut(s)	3744
BsrI	ACTGG	3 cut(s)	1964, 2719, 3450
BssECI	CCNNGG	8 cut(s)	51, 85, 360, 637, 638, 1641, 2023, 2901
BssNAI	GTATAC	1 cut(s)	3402
BssT1I	CCWWGG	5 cut(s)	51, 85, 1641, 2023, 2901
Bst1107I	GTATAC	1 cut(s)	3402
Bst4CI	ACNGT	3 cut(s)	716, 2912, 3444
Bst6I	CTCTTC	3 cut(s)	413, 2458, 3802
BstAUI	TGTACA	1 cut(s)	3744
BstBAI	YACGTR	1 cut(s)	898
BstBI	TTCGAA	4 cut(s)	2752, 2794, 3305, 3726
BstC8I	GCNNGC	4 cut(s)	1192, 2165, 3328, 3681
BstDEI	CTNAG	6 cut(s)	75, 188, 2304, 2648, 2726, 3322
BstDSI	CCRYGG	2 cut(s)	51, 360
BstF5I	GGATG	9 cut(s)	514, 532, 541, 580, 2231, 2802, 3194, 3343, 3370
BstFNI	CGCG	2 cut(s)	159, 1000
BstH2I	RGCGCY	3 cut(s)	157, 207, 647
BstHHI	GCGC	4 cut(s)	156, 161, 206, 646
BstMAI	GTCTC	3 cut(s)	1132, 1896, 3515
BstMWI	GCNNNNNNNGC	5 cut(s)	198, 212, 1578, 3391, 3478
BstNSI	RCATGY	2 cut(s)	2919, 3072
BstSCI	CCNGG	2 cut(s)	636, 637
BstSFI	CTRYAG	3 cut(s)	312, 916, 957
BstSLI	GKGCMC	3 cut(s)	261, 721, 1706
BstSNI	TACGTA	1 cut(s)	898
BstUI	CGCG	2 cut(s)	159, 1000
BstV1I	GCAGC	1 cut(s)	166
BstV2I	GAAGAC	4 cut(s)	86, 2115, 2429, 3747
BstX2I	RGATCY	3 cut(s)	1520, 2494, 3614
BstXI	CCANNNNNNTGG	1 cut(s)	728
BstYI	RGATCY	3 cut(s)	1520, 2494, 3614
BstZ17I	GTATAC	1 cut(s)	3402
BsuRI	GGCC	7 cut(s)	1639, 1845, 1963, 2028, 2177, 2187, 3182
BtgI	CCRYGG	2 cut(s)	51, 360
BtrI	CACGTC	2 cut(s)	1030, 2181
BtsCI	GGATG	9 cut(s)	514, 532, 541, 580, 2231, 2802, 3194, 3343, 3370
BtsI	GCAGTG	3 cut(s)	301, 321, 3087
BtsIMutI	CAGTG	5 cut(s)	301, 321, 1957, 3087, 3457
BtuMI	TCGCGA	1 cut(s)	1000
BveI	ACCTGC	1 cut(s)	577
Cac8I	GCNNGC	4 cut(s)	1192, 2165, 3328, 3681
CaiI	CAGNNNCTG	2 cut(s)	320, 3397
CciI	TCATGA	2 cut(s)	604, 2262
CfoI	GCGC	4 cut(s)	156, 161, 206, 646
Cfr13I	GGNCC	3 cut(s)	1638, 2536, 2621
Cfr9I	CCCGGG	1 cut(s)	637
Csp6I	GTAC	7 cut(s)	382, 1256, 1981, 2402, 2913, 3319, 3745
CspCI	CAANNNNNGTGG	2 cut(s)	3363, 3398
CviQI	GTAC	7 cut(s)	382, 1256, 1981, 2402, 2913, 3319, 3745
DdeI	CTNAG	6 cut(s)	75, 188, 2304, 2648, 2726, 3322
DraI	TTTAAA	4 cut(s)	1332, 1338, 1447, 3535
DraIII	CACNNNGTG	2 cut(s)	833, 1018
DriI	GACNNNNNGTC	2 cut(s)	87, 3231
EaeI	YGGCCR	4 cut(s)	1843, 2175, 2185, 3180
Eam1104I	CTCTTC	3 cut(s)	413, 2458, 3802
Eam1105I	GACNNNNNGTC	2 cut(s)	87, 3231
EarI	CTCTTC	3 cut(s)	413, 2458, 3802
Eco105I	TACGTA	1 cut(s)	898
Eco130I	CCWWGG	5 cut(s)	51, 85, 1641, 2023, 2901
Eco147I	AGGCCT	1 cut(s)	2028
Eco31I	GGTCTC	1 cut(s)	1132
Eco47I	GGWCC	2 cut(s)	2536, 2621
Eco57I	CTGAAG	2 cut(s)	2438, 3012
Eco88I	CYCGRG	2 cut(s)	637, 1135
EcoO109I	RGGNCCY	1 cut(s)	1638
EcoRI	GAATTC	2 cut(s)	2364, 2408
EcoT14I	CCWWGG	5 cut(s)	51, 85, 1641, 2023, 2901
EcoT22I	ATGCAT	1 cut(s)	2933
ErhI	CCWWGG	5 cut(s)	51, 85, 1641, 2023, 2901
FalI	AAGNNNNNCTT	2 cut(s)	1415, 1447
FaqI	GGGAC	3 cut(s)	640, 984, 1396
FauNDI	CATATG	2 cut(s)	1179, 3483
FbaI	TGATCA	3 cut(s)	561, 2529, 2731
FblI	GTMKAC	2 cut(s)	2094, 3401
Fnu4HI	GCNGC	5 cut(s)	157, 180, 202, 444, 594
FokI	GGATG	9 cut(s)	521, 539, 548, 587, 2218, 2789, 3181, 3350, 3357
Fsp4HI	GCNGC	5 cut(s)	157, 180, 202, 444, 594
GlaI	GCGC	4 cut(s)	155, 160, 205, 645
GluI	GCNGC	5 cut(s)	157, 180, 202, 444, 594
HaeII	RGCGCY	3 cut(s)	157, 207, 647
HaeIII	GGCC	7 cut(s)	1639, 1845, 1963, 2028, 2177, 2187, 3182
HapII	CCGG	3 cut(s)	638, 659, 2184
HhaI	GCGC	4 cut(s)	156, 161, 206, 646
Hin6I	GCGC	4 cut(s)	154, 159, 204, 644
HinP1I	GCGC	4 cut(s)	154, 159, 204, 644
HincII	GTYRAC	1 cut(s)	2095
HindII	GTYRAC	1 cut(s)	2095
HindIII	AAGCTT	2 cut(s)	1579, 2269
HpaII	CCGG	3 cut(s)	638, 659, 2184
HphI	GGTGA	8 cut(s)	40, 133, 500, 1004, 1384, 1843, 1988, 2872
Hpy166II	GTNNAC	7 cut(s)	24, 271, 1027, 1856, 2095, 2908, 3402
Hpy188I	TCNGA	7 cut(s)	322, 893, 1126, 1724, 2104, 2305, 2958
Hpy8I	GTNNAC	7 cut(s)	24, 271, 1027, 1856, 2095, 2908, 3402
HpyAV	CCTTC	8 cut(s)	154, 169, 451, 1180, 1884, 2079, 3184, 3373
HpyCH4III	ACNGT	3 cut(s)	716, 2912, 3444
HpyCH4IV	ACGT	5 cut(s)	629, 897, 1029, 1254, 2180
HpyF10VI	GCNNNNNNNGC	5 cut(s)	198, 212, 1578, 3391, 3478
HpyF3I	CTNAG	6 cut(s)	75, 188, 2304, 2648, 2726, 3322
HpySE526I	ACGT	5 cut(s)	629, 897, 1029, 1254, 2180
HspAI	GCGC	4 cut(s)	154, 159, 204, 644
Ksp22I	TGATCA	3 cut(s)	561, 2529, 2731
LguI	GCTCTTC	1 cut(s)	2458
LmnI	GCTCC	5 cut(s)	807, 2209, 2333, 2597, 3418
Lsp1109I	GCAGC	1 cut(s)	166
LweI	GCATC	3 cut(s)	517, 3115, 3814
MaeII	ACGT	5 cut(s)	629, 897, 1029, 1254, 2180
MaeIII	GTNAC	8 cut(s)	139, 301, 710, 898, 1150, 2432, 2818, 3064
MfeI	CAATTG	1 cut(s)	2313
MflI	RGATCY	3 cut(s)	1520, 2494, 3614
MhlI	GDGCHC	3 cut(s)	261, 721, 1706
MlsI	TGGCCA	3 cut(s)	1845, 2177, 3182
MluNI	TGGCCA	3 cut(s)	1845, 2177, 3182
MlyI	GAGTC	3 cut(s)	1066, 1378, 3038
MmeI	TCCRAC	4 cut(s)	384, 513, 1295, 1780
Mox20I	TGGCCA	3 cut(s)	1845, 2177, 3182
Mph1103I	ATGCAT	1 cut(s)	2933
MroXI	GAANNNNTTC	1 cut(s)	3153
MscI	TGGCCA	3 cut(s)	1845, 2177, 3182
MslI	CAYNNNNRTG	4 cut(s)	1974, 2842, 2983, 3062
Msp20I	TGGCCA	3 cut(s)	1845, 2177, 3182
MspA1I	CMGCKG	1 cut(s)	2043
MspI	CCGG	3 cut(s)	638, 659, 2184
MspR9I	CCNGG	2 cut(s)	638, 639
MteI	GCGCNGCGC	1 cut(s)	157
MunI	CAATTG	1 cut(s)	2313
Mva1269I	GAATGC	2 cut(s)	2258, 2519
MvnI	CGCG	2 cut(s)	159, 1000
MwoI	GCNNNNNNNGC	5 cut(s)	198, 212, 1578, 3391, 3478
NciI	CCSGG	2 cut(s)	638, 639
NcoI	CCATGG	1 cut(s)	51
NdeI	CATATG	2 cut(s)	1179, 3483
NheI	GCTAGC	1 cut(s)	3679
NlaIV	GGNNCC	6 cut(s)	260, 357, 718, 1522, 1952, 3812
NmuCI	GTSAC	4 cut(s)	139, 710, 2818, 3064
NruI	TCGCGA	1 cut(s)	1000
NsiI	ATGCAT	1 cut(s)	2933
NspI	RCATGY	2 cut(s)	2919, 3072
NspV	TTCGAA	4 cut(s)	2752, 2794, 3305, 3726
OliI	CACNNNNGTG	1 cut(s)	1974
PacI	TTAATTAA	1 cut(s)	2856
PaeR7I	CTCGAG	1 cut(s)	1135
PagI	TCATGA	2 cut(s)	604, 2262
PceI	AGGCCT	1 cut(s)	2028
PciI	ACATGT	1 cut(s)	2915
PciSI	GCTCTTC	1 cut(s)	2458
PcsI	WCGNNNNNNNCGW	1 cut(s)	2100
PctI	GAATGC	2 cut(s)	2258, 2519
PdmI	GAANNNNTTC	1 cut(s)	3153
PfeI	GAWTC	7 cut(s)	655, 1428, 1440, 1670, 1713, 3035, 3245
PflFI	GACNNNGTC	1 cut(s)	675
PflMI	CCANNNNNTGG	1 cut(s)	2194
PkrI	GCNGC	5 cut(s)	158, 181, 203, 445, 595
PleI	GAGTC	3 cut(s)	1066, 1378, 3038
PpsI	GAGTC	3 cut(s)	1066, 1378, 3038
Ppu21I	YACGTR	1 cut(s)	898
PscI	ACATGT	1 cut(s)	2915
PshBI	ATTAAT	1 cut(s)	852
PspN4I	GGNNCC	6 cut(s)	260, 357, 718, 1522, 1952, 3812
PspPI	GGNCC	3 cut(s)	1638, 2536, 2621
PstI	CTGCAG	2 cut(s)	316, 961
PstNI	CAGNNNCTG	2 cut(s)	320, 3397
PsuI	RGATCY	3 cut(s)	1520, 2494, 3614
PsyI	GACNNNGTC	1 cut(s)	675
RruI	TCGCGA	1 cut(s)	1000
RsaI	GTAC	7 cut(s)	383, 1257, 1982, 2403, 2914, 3320, 3746
RsaNI	GTAC	7 cut(s)	382, 1256, 1981, 2402, 2913, 3319, 3745
RseI	CAYNNNNRTG	4 cut(s)	1974, 2842, 2983, 3062
SalI	GTCGAC	1 cut(s)	2093
SapI	GCTCTTC	1 cut(s)	2458
SatI	GCNGC	5 cut(s)	157, 180, 202, 444, 594
Sau96I	GGNCC	3 cut(s)	1638, 2536, 2621
SchI	GAGTC	3 cut(s)	1066, 1378, 3038
ScrFI	CCNGG	2 cut(s)	638, 639
SduI	GDGCHC	3 cut(s)	261, 721, 1706
SfaNI	GCATC	3 cut(s)	517, 3115, 3814
SfcI	CTRYAG	3 cut(s)	312, 916, 957
Sfr274I	CTCGAG	1 cut(s)	1135
SfuI	TTCGAA	4 cut(s)	2752, 2794, 3305, 3726
SinI	GGWCC	2 cut(s)	2536, 2621
SlaI	CTCGAG	1 cut(s)	1135
SmaI	CCCGGG	1 cut(s)	639
SmiMI	CAYNNNNRTG	4 cut(s)	1974, 2842, 2983, 3062
SmlI	CTYRAG	4 cut(s)	116, 1135, 1613, 2558
SmoI	CTYRAG	4 cut(s)	116, 1135, 1613, 2558
SnaBI	TACGTA	1 cut(s)	898
SseBI	AGGCCT	1 cut(s)	2028
SspI	AATATT	4 cut(s)	43, 1540, 2639, 2948
StuI	AGGCCT	1 cut(s)	2028
StyD4I	CCNGG	2 cut(s)	636, 637
StyI	CCWWGG	5 cut(s)	51, 85, 1641, 2023, 2901
TaaI	ACNGT	3 cut(s)	716, 2912, 3444
TaiI	ACGT	5 cut(s)	632, 900, 1032, 1257, 2183
TatI	WGTACW	5 cut(s)	381, 2401, 2912, 3318, 3744
TauI	GCSGC	4 cut(s)	159, 204, 446, 596
TfiI	GAWTC	7 cut(s)	655, 1428, 1440, 1670, 1713, 3035, 3245
TscAI	CASTG	5 cut(s)	301, 321, 1964, 3094, 3457
TseFI	GTSAC	4 cut(s)	139, 710, 2818, 3064
TseI	GCWGC	1 cut(s)	179
Tsp45I	GTSAC	4 cut(s)	139, 710, 2818, 3064
TspMI	CCCGGG	1 cut(s)	637
TspRI	CASTG	5 cut(s)	301, 321, 1964, 3094, 3457
Tth111I	GACNNNGTC	1 cut(s)	675
Van91I	CCANNNNNTGG	1 cut(s)	2194
VpaK11BI	GGWCC	2 cut(s)	2536, 2621
VspI	ATTAAT	1 cut(s)	852
XbaI	TCTAGA	2 cut(s)	280, 2497
XceI	RCATGY	2 cut(s)	2919, 3072
XhoI	CTCGAG	1 cut(s)	1135
XmaI	CCCGGG	1 cut(s)	637
XmiI	GTMKAC	2 cut(s)	2094, 3401
XmnI	GAANNNNTTC	1 cut(s)	3153
Zsp2I	ATGCAT	1 cut(s)	2933

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.