Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0005903

Physical Location & Seq

Genomic Coordinates Forward (+)

44907917 .. 44911753

Gene Structure 3837 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0296300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3699 bp

ATGAACGCAGCTCAGGAGACTACTCTGCGTCTCCTCCTCCTCCATATTTTCTTGCTCTTTTTGCTTCGATTGGAGGCCACATCATCACAAAGAATACAAGCTGAAGCTCTCCTAAAATGGAAGAACAGCTTTACTTCTTCACCACCTTCTCTGAATTCATGGTCGCTCACAAACCTAAACAACCTGTGCAACTGGACTGCCATTGTCTGCAACCGCAATACCAAAACAGTTTCTGAAATTCACCTTGCAAACTTCAACATCACTGCAGCACTAACGCAGTTCAACTTCGACAAGTTTCTCAATATCACCCATTTCAACCTCAATGGCAACAATTTCTCAGGGCCTATACCATCTGCCATTGGCAACCTCACCAAGCTCACCACCTTGGACTTGGGAAACAATAACTTGGAACGAGAAGTGCCTGCGGAAATGGGCAAGTTAACGGAGCTTCAGTTTCTAAGTCTCTACAGCAACAATATCAATGGTACCATTCCCTTTCAGCTGGACAATCTCAAAAAGGTACAATATTTTCTTCTTGGAAGTAACCACTTAGAGCCTGCTGATTGGTCCAACTTTTCAGGCTTTCCTGTGTTGACATTCCTTGATCTTTCTATGAACAAACTAGACTCAAAATTTCCAGAATTTATATCTGAATGTAGGAACTTGACATTCCTAGATTTGTCTCAAAATGCTTTGACTGGCCAAATACCAGAAGTGGTATTTACCAATCTGGTCAAGCTTGAATATCTCAATCTCACCAATAACCAATTCCAAGGGCCAATGCCATATCACTTTCCCAACCTCAAACACCTTTATTTGGGTGTGAACAACTTTAGTGGTCCAATTCCTGAAGATATAGGTCTGTTATCTGGTCTGGAAATTTTTGAAGTGCTTAACAATTCCCTTGAAGGGAAAATCCCATCCTCTATAGGCCAACTCAAGGAGCTTCAGCGCCTTGATTTTAGATCAAATTCATTAAACTCTTCAATCCCTTCTGAACTTGGTTCATGTACCAACCTCACTTTCTTTGCATTGGCTTTGAATAAACTCAGTGGGGAACTGCCTCTGTCCTTGTCCAATCTCAACAGCTTGATGGAGTTGGGGTTATCTGATAATCTATTTACTGGGCCAATCTTGCCTTCTCTGGTCTCCAATTGGACTAAAATGATCTCTTTGCAGCTCCAAAACAATAGCTTCAGTGGGAATATTCCACCAGAAATTGGCCTATTAAAAAAACTTGATTTCCTGTTTCTGTACAACAACAATTTCTCTGGATCAATTCCCTCAGAGATAGGAAACTTGCAAGTTTTGACAAATTTGGATCTCTCTACAAACCAGCTCTCTGGGCCGATTCCTATGAAATTCTGGAACCTCACAAATCTTCAATACGCTGTCTTTTTCAACAACAGTCTCTGCGGCAATATCCCCCCAGAGATTGGAAACATGAGTTCCATTGTTTCCTTTGAAGTCAGCACAAACCAACTTGAAGGGGAGTTGCCGAAAACTATTTCTCACCTCAGAAAACTAGAGTGCTTTTACGTTTTCACTAATAGGTTTTCAGGGACCATTCCCAGTGATTTCGGGAAGTATAGTCCTAACTTGAGAGTTGTTAGCTTATGGGAGAACAGCTTCTCTGGAGAATTGCCGCCAGAGTTGTGTAGTGGATTTGCTTTGCAAAAATTGAGAGTGCACAGGAACAACTTCACAGGGCCATTGCCTGAGTGCTTGAGAAATTGTTCTGCATTAACAAATGTTCGGCTGGAAGTAAACCAATTCACCGGAAACATTACCAATGCATTTGGTGTTCATCCCCGTCTTGTATCAATTCATCTTAGTCACAACAAGTTTGTTGGTCAACTCTCGCCACAATGGGGAGAATGCAAAAACATTACGGATATGCAAATGGACGGAAATAGAATTTCTGGTCAAATCCCACCTGAGCTTGGCCAGTTGACACAGTTGCAGTATCTAACTCTAAGCTCTAATGAATTCAGTGGGGAATTTCCCGTTTCCATCGGCAATCTAAGCTTGTTGTTTACTCTTAATCTCAGCAGGAACCACTTGACGGGAACTTTCCCTCATGTTCTAGGCAAATTGACTAAGCTCGAGCTTCTTGATTTGTCTGATAATAATTTTACAGGGGTATTACCAGTCGACCCTGGAACGTTTCAGAGGTTAACAAGCTTGAACATAAGCCACAACAAATTATCAGGTGGAATACCTGAGGAGATTGGTAATCTACACCTGCAGTACTTGTTGGACTTAAGCAGCAATTCGTTCTCGGGATCACTACCGTTAAACTTTGCCAAGCTTATAATGCTGGAGATTCTTAATGTCTCGCATAACCAACTCTCCGGGAATATCCCATCAACATTTTCCAGAATGGTAAGTCTTGTCGGCATTGATTTTTCTTACAACAACTTGACAGGTCCAATCCCAACTGGTGGCATTTTTCAAAAGAAGCTTCCGAATACTTTCATTGGAAATATTGGTTTGTGTGGTGAAACAGAGGGACTAACTCCATGCACTTCGGGTCCCATAAAGTCCAACAAGAAAATTCTTGTTGGCGTCCTTGTTCCCATTTTTGCTATATTGCTCATGGTGACCATATCTGCTGTAATCCTTAAGTTCTGCAAGAAATCCAAGCTTGGAGAATTCGATCATCCTAAGGTATCTGAAAGTTTTGATTCAATTATTTGGGGAAGACATGGTAAATTTAGATTTTCTGAAATCATCACTGCCACCGAGAACTTTGATGAAAAGTACCTCATTGGAAGAGGGGGTTTTGGAAGTGTCTACAAGGCTGTGTTGGACAGAGGCAAAGTCGTTGCAGTTAAGAAATTGAATATGTCAGAAGATTCTAGTGACGACATTGCAGAATTGAATCGCAAGAGTTTTGAGAATGAGATAAGAACTTTGACAGAAGTGAAGCACAGAAATGTAATAAAACTTTATGGGTTCTGCTCCTGGAAGGGGTGCTTGCACTTGGTGTATGAATATGCAGAGAGAGGCAGTTTGAAAATGGTATTGTATGGAGTGGAGGGGGAAGCAGAAGTACTTGGGTGGGGCACGAGGGTGAAAGTTGTGCAAGGAATAGCTCATGCTATTTCTCACTTGCATAATGATTGCTCTCACCCAATCATTCACCGCGACATAACTTTGAGCAACATATTACTAGATCGAGATTTCGTGCCACGCCTATCTGACTTTGGAATAGCAAGGCTATTGAGCTCAGATTCCTCAAACTGGACTGCTGTAGCGGGGTCTTATGGCTACATGGCTCCAGAGCTCGCATTCACAATGCAAGTGACAGATAAGTGTGATGTATACAGCTTTGGGGTGGTGGCATTAGAAATCATGATGGGAAGGCATCCAGGGGAGCTACTGACTTCTCTATCAGAAAATGGAGAATTGCTTTTGAAGGATTTGCTAGACCAACGACTTCGTTCTCCTTCAAGTCAATTAGCAGTGCGAGTGGTTTCCTTAGTTACAATGGCCTTGGCCTGCGCCAGCACTAATCCGGAATCACGACCCACCATGCATTTTGTGGCAAAAGAACTATCATCAACCAAGACCCGGACTTGCCTTTCTGAGCCGTTCAGTACGATAACCATCAATAAATTGGCAAGTTCTCATAACTGGGAAGTTGCTGGAATAAAAAAATGGGGTTTAACTACTAAATCCCTTCTCCAAAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0009791 GO:0009888 GO:0009889 GO:0009960 GO:0010154 GO:0010468 GO:0010556 GO:0010817 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032879 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051049 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0065008 GO:0080090 GO:0140110 GO:1903506 GO:2000012 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04010 ko04011 ko04013 ko04022 ko04111 ko04371 ko04921 ko05202 ko05418 map04010 map04011 map04013 map04022 map04111 map04371 map04921 map05202 map05418

Pfam Domains

Protein Families

SRF-TF

Protein Analysis

1232

Amino Acids

136.57

Weight (kDa)

6.57

Isoelectric Point (pI)

34.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	33 - 71	2.3e-06	Leucine rich repeat N-terminal domain
LRR_14	PF23598	102 - 175	1.2e-06	Leucine-rich repeat region
LRR_8	PF13855	174 - 232	2.2e-07	Leucine rich repeat
LRR_8	PF13855	198 - 257	2.4e-08	Leucine rich repeat
LRR_14	PF23598	266 - 369	6.9e-07	Leucine-rich repeat region
LRR_14	PF23598	456 - 625	4.6e-06	Leucine-rich repeat region
LRR_14	PF23598	647 - 746	2e-08	Leucine-rich repeat region
Pkinase	PF00069	920 - 1182	8.8e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	923 - 1187	6.8e-41	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005903)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g40280 FvH4_4g35860
malus_domestica	MD02G1221000.v1.1 MD15G1322300.v1.1
prunus_persica	Prupe.2G120200_v2.0.a1
pyrus_communis	pycom02g18540
rosa_chinensis	RchiOBHm_Chr3g0480601 RchiOBHm_Chr4g0445441 RchiOBHm_Chr6g0300471
rosa_laevigata	RLG00000005740 RLG00000011335 RLG00000012216
rosa_multiflora	Rmu_sc0001476.1_g000003 Rmu_sc0001509.1_g000008
rosa_roxburghii	Rroxscaffold_5G00385950 Rroxscaffold_7G00167760
rosa_rugosa	Rorug04G0361300 Rorug06G0296300
rosa_samantha	Rh4AG422800 Rh4BG434800 Rh4CG449700 Rh4DG431000 Rh6AG408900 Rh6BG416200 Rh6CG423700 Rh6DG409400
rosa_wichuraiana	Rw4G036260 Rw6G035780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2315
AarI	CACCTGC	1 cut(s)	2253
Acc36I	ACCTGC	1 cut(s)	2253
Acc65I	GGTACC	1 cut(s)	485
AccB1I	GGYRCC	1 cut(s)	485
AccB7I	CCANNNNNTGG	3 cut(s)	1220, 1436, 2444
AccI	GTMKAC	3 cut(s)	2154, 2796, 3327
AccII	CGCG	1 cut(s)	3150
AccIII	TCCGGA	1 cut(s)	3520
AciI	CCGC	6 cut(s)	214, 425, 1416, 1646, 3148, 3260
AclI	AACGTT	1 cut(s)	2165
AclWI	GGATC	3 cut(s)	1282, 1329, 2293
AcoI	YGGCCR	2 cut(s)	700, 1945
AcuI	CTGAAG	5 cut(s)	123, 434, 870, 932, 1180
AcyI	GRCGYC	1 cut(s)	2568
AdeI	CACNNNGTG	1 cut(s)	2989
AfaI	GTAC	8 cut(s)	487, 522, 1012, 1256, 2252, 2765, 3057, 3604
AflII	CTTAAG	2 cut(s)	2263, 2624
AjnI	CCWGG	3 cut(s)	2158, 2966, 3373
AjuI	GAANNNNNNNTTGG	6 cut(s)	752, 784, 1007, 1039, 1123, 1155
AleI	CACNNNNGTG	1 cut(s)	3074
Alw21I	GWGCWC	3 cut(s)	1692, 3233, 3291
Alw26I	GTCTC	7 cut(s)	11, 35, 467, 687, 1153, 1415, 2341
Alw44I	GTGCAC	1 cut(s)	1688
AlwI	GGATC	3 cut(s)	1282, 1329, 2293
AlwNI	CAGNNNCTG	2 cut(s)	233, 1413
Ama87I	CYCGRG	2 cut(s)	2105, 2281
Aor13HI	TCCGGA	1 cut(s)	3520
ApaLI	GTGCAC	1 cut(s)	1688
ApeKI	GCWGC	4 cut(s)	8, 266, 1177, 2268
Asp700I	GAANNNNTTC	4 cut(s)	1628, 1700, 1735, 2474
Asp718I	GGTACC	1 cut(s)	485
AspLEI	GCGC	2 cut(s)	954, 3509
AsuC2I	CCSGG	2 cut(s)	2356, 3577
AvaI	CYCGRG	2 cut(s)	2105, 2281
AvaII	GGWCC	5 cut(s)	567, 839, 1563, 2429, 2534
AxyI	CCTNAGG	2 cut(s)	2223, 2667
BaeGI	GKGCMC	2 cut(s)	1692, 3071
BalI	TGGCCA	2 cut(s)	702, 1947
BanI	GGYRCC	1 cut(s)	485
BanII	GRGCYC	2 cut(s)	3233, 3291
BarI	GAAGNNNNNNTAC	2 cut(s)	3039, 3071
BauI	CACGAG	1 cut(s)	3070
BbsI	GAAGAC	1 cut(s)	2710
Bbv12I	GWGCWC	3 cut(s)	1692, 3233, 3291
BbvI	GCAGC	4 cut(s)	20, 278, 1189, 2280
BccI	CCATC	7 cut(s)	358, 928, 1087, 2021, 2374, 3355, 3620
BceAI	ACGGC	1 cut(s)	3580
BciT130I	CCWGG	3 cut(s)	2160, 2968, 3375
BcnI	CCSGG	2 cut(s)	2356, 3577
BcoDI	GTCTC	7 cut(s)	11, 35, 467, 687, 1153, 1415, 2341
BfaI	CTAG	7 cut(s)	623, 674, 1526, 2087, 2862, 3176, 3431
BfmI	CTRYAG	5 cut(s)	264, 466, 927, 2246, 3255
BfoI	RGCGCY	1 cut(s)	955
BfrI	CTTAAG	2 cut(s)	2263, 2624
BfuAI	ACCTGC	1 cut(s)	2253
BisI	GCNGC	6 cut(s)	9, 267, 1178, 1417, 1646, 2269
BlsI	GCNGC	6 cut(s)	10, 268, 1179, 1418, 1647, 2270
BmcAI	AGTACT	2 cut(s)	2252, 3057
Bme1390I	CCNGG	5 cut(s)	2160, 2356, 2968, 3375, 3577
Bme18I	GGWCC	5 cut(s)	567, 839, 1563, 2429, 2534
BmeT110I	CYCGRG	2 cut(s)	2105, 2281
BmiI	GGNNCC	7 cut(s)	487, 1370, 1564, 2057, 2535, 2536, 3282
BmrFI	CCNGG	5 cut(s)	2160, 2356, 2968, 3375, 3577
BmrI	ACTGGG	3 cut(s)	1134, 1566, 3649
BmsI	GCATC	1 cut(s)	3379
BmuI	ACTGGG	3 cut(s)	1134, 1566, 3649
BpiI	GAAGAC	1 cut(s)	2710
BpmI	CTGGAG	3 cut(s)	1656, 2342, 3267
Bpu10I	CCTNAGC	2 cut(s)	12, 1938
BpuEI	CTTGAG	3 cut(s)	923, 1621, 1747
BpuMI	CCSGG	2 cut(s)	2356, 3577
BsaBI	GATNNNNATC	1 cut(s)	3611
BsaHI	GRCGYC	1 cut(s)	2568
BsaI	GGTCTC	1 cut(s)	1153
BsaJI	CCNNGG	5 cut(s)	384, 772, 2158, 3374, 3498
BsaWI	WCCGGW	2 cut(s)	1778, 3520
BsaXI	ACNNNNNCTCC	6 cut(s)	437, 467, 1088, 1118, 2336, 2366
Bse1I	ACTGG	9 cut(s)	197, 703, 1129, 1572, 1948, 2150, 2446, 3251, 3644
Bse21I	CCTNAGG	2 cut(s)	2223, 2667
Bse3DI	GCAATG	2 cut(s)	1712, 2871
Bse8I	GATNNNNATC	1 cut(s)	3611
BseAI	TCCGGA	1 cut(s)	3520
BseBI	CCWGG	3 cut(s)	2160, 2968, 3375
BseDI	CCNNGG	5 cut(s)	384, 772, 2158, 3374, 3498
BseGI	GGATG	4 cut(s)	920, 1807, 2662, 3370
BseJI	GATNNNNATC	1 cut(s)	3611
BseMI	GCAATG	2 cut(s)	1712, 2871
BseNI	ACTGG	9 cut(s)	197, 703, 1129, 1572, 1948, 2150, 2446, 3251, 3644
BseRI	GAGGAG	4 cut(s)	23, 26, 29, 2240
BseSI	GKGCMC	2 cut(s)	1692, 3071
BseXI	GCAGC	4 cut(s)	20, 278, 1189, 2280
Bsh1236I	CGCG	1 cut(s)	3150
BshNI	GGYRCC	1 cut(s)	485
BsiHKAI	GWGCWC	3 cut(s)	1692, 3233, 3291
BsiHKCI	CYCGRG	2 cut(s)	2105, 2281
BsiSI	CCGG	4 cut(s)	1779, 2355, 3521, 3577
BslFI	GGGAC	3 cut(s)	1576, 2520, 2526
BsmAI	GTCTC	7 cut(s)	11, 35, 467, 687, 1153, 1415, 2341
BsmBI	CGTCTC	1 cut(s)	35
BsmFI	GGGAC	3 cut(s)	1576, 2520, 2526
BsmI	GAATGC	2 cut(s)	1883, 3293
Bso31I	GGTCTC	1 cut(s)	1153
BsoBI	CYCGRG	2 cut(s)	2105, 2281
Bsp1286I	GDGCHC	4 cut(s)	1692, 3071, 3233, 3291
Bsp13I	TCCGGA	1 cut(s)	3520
Bsp1407I	TGTACA	1 cut(s)	1254
Bsp143I	GATC	8 cut(s)	604, 965, 1167, 1274, 1321, 2285, 2659, 3178
BspACI	CCGC	6 cut(s)	214, 425, 1416, 1646, 3148, 3260
BspEI	TCCGGA	1 cut(s)	3520
BspFNI	CGCG	1 cut(s)	3150
BspHI	TCATGA	1 cut(s)	3357
BspLI	GGNNCC	7 cut(s)	487, 1370, 1564, 2057, 2535, 2536, 3282
BspMAI	CTGCAG	2 cut(s)	268, 2250
BspMI	ACCTGC	1 cut(s)	2253
BspPI	GGATC	3 cut(s)	1282, 1329, 2293
BspT107I	GGYRCC	1 cut(s)	485
BspTI	CTTAAG	2 cut(s)	2263, 2624
BspTNI	GGTCTC	1 cut(s)	1153
BsrDI	GCAATG	2 cut(s)	1712, 2871
BsrGI	TGTACA	1 cut(s)	1254
BsrI	ACTGG	9 cut(s)	197, 703, 1129, 1572, 1948, 2150, 2446, 3251, 3644
BssECI	CCNNGG	5 cut(s)	384, 772, 2158, 3374, 3498
BssMI	GATC	8 cut(s)	604, 965, 1167, 1274, 1321, 2285, 2659, 3178
BssNAI	GTATAC	1 cut(s)	3328
BssNI	GRCGYC	1 cut(s)	2568
BssSI	CACGAG	1 cut(s)	3070
BssT1I	CCWWGG	3 cut(s)	384, 772, 3498
Bst1107I	GTATAC	1 cut(s)	3328
Bst2BI	CACGAG	1 cut(s)	3070
Bst2UI	CCWGG	3 cut(s)	2160, 2968, 3375
Bst4CI	ACNGT	4 cut(s)	229, 1409, 1959, 2295
Bst6I	CTCTTC	2 cut(s)	988, 2770
BstACI	GRCGYC	1 cut(s)	2568
BstAFI	CTTAAG	2 cut(s)	2263, 2624
BstAUI	TGTACA	1 cut(s)	1254
BstC8I	GCNNGC	6 cut(s)	423, 558, 2981, 3291, 3505, 3511
BstEII	GGTNACC	1 cut(s)	2602
BstF5I	GGATG	4 cut(s)	920, 1807, 2662, 3370
BstFNI	CGCG	1 cut(s)	3150
BstH2I	RGCGCY	1 cut(s)	955
BstHHI	GCGC	2 cut(s)	954, 3509
BstKTI	GATC	8 cut(s)	607, 968, 1170, 1277, 1324, 2288, 2662, 3181
BstMAI	GTCTC	7 cut(s)	11, 35, 467, 687, 1153, 1415, 2341
BstMBI	GATC	8 cut(s)	604, 965, 1167, 1274, 1321, 2285, 2659, 3178
BstMWI	GCNNNNNNNGC	5 cut(s)	61, 1135, 1345, 2025, 2317
BstNI	CCWGG	3 cut(s)	2160, 2968, 3375
BstPI	GGTNACC	1 cut(s)	2602
BstSCI	CCNGG	5 cut(s)	2158, 2354, 2966, 3373, 3575
BstSFI	CTRYAG	5 cut(s)	264, 466, 927, 2246, 3255
BstSLI	GKGCMC	2 cut(s)	1692, 3071
BstUI	CGCG	1 cut(s)	3150
BstV1I	GCAGC	4 cut(s)	20, 278, 1189, 2280
BstV2I	GAAGAC	1 cut(s)	2710
BstX2I	RGATCY	1 cut(s)	1321
BstXI	CCANNNNNNTGG	1 cut(s)	1343
BstYI	RGATCY	1 cut(s)	1321
BstZ17I	GTATAC	1 cut(s)	3328
Bsu36I	CCTNAGG	2 cut(s)	2223, 2667
BtsCI	GGATG	4 cut(s)	920, 1807, 2662, 3370
BtsI	GCAGTG	3 cut(s)	261, 2736, 3474
BtsIMutI	CAGTG	7 cut(s)	261, 1057, 1204, 1579, 1999, 2736, 3474
BveI	ACCTGC	1 cut(s)	2253
Cac8I	GCNNGC	6 cut(s)	423, 558, 2981, 3291, 3505, 3511
CaiI	CAGNNNCTG	2 cut(s)	233, 1413
CciI	TCATGA	1 cut(s)	3357
CfoI	GCGC	2 cut(s)	954, 3509
CseI	GACGC	2 cut(s)	17, 2557
Csp6I	GTAC	8 cut(s)	486, 521, 1011, 1255, 2251, 2764, 3056, 3603
CspCI	CAANNNNNGTGG	2 cut(s)	817, 852
CviQI	GTAC	8 cut(s)	486, 521, 1011, 1255, 2251, 2764, 3056, 3603
DpnI	GATC	8 cut(s)	606, 967, 1169, 1276, 1323, 2287, 2661, 3180
DpnII	GATC	8 cut(s)	604, 965, 1167, 1274, 1321, 2285, 2659, 3178
DraIII	CACNNNGTG	1 cut(s)	2989
EaeI	YGGCCR	2 cut(s)	700, 1945
Eam1104I	CTCTTC	2 cut(s)	988, 2770
EarI	CTCTTC	2 cut(s)	988, 2770
Ecl136II	GAGCTC	2 cut(s)	3231, 3289
Eco130I	CCWWGG	3 cut(s)	384, 772, 3498
Eco24I	GRGCYC	2 cut(s)	3233, 3291
Eco31I	GGTCTC	1 cut(s)	1153
Eco47I	GGWCC	5 cut(s)	567, 839, 1563, 2429, 2534
Eco53kI	GAGCTC	2 cut(s)	3231, 3289
Eco57I	CTGAAG	5 cut(s)	123, 434, 870, 932, 1180
Eco81I	CCTNAGG	2 cut(s)	2223, 2667
Eco88I	CYCGRG	2 cut(s)	2105, 2281
Eco91I	GGTNACC	1 cut(s)	2602
EcoICRI	GAGCTC	2 cut(s)	3231, 3289
EcoO109I	RGGNCCY	2 cut(s)	341, 2534
EcoO65I	GGTNACC	1 cut(s)	2602
EcoRI	GAATTC	3 cut(s)	154, 1988, 2654
EcoRII	CCWGG	3 cut(s)	2158, 2966, 3373
EcoT14I	CCWWGG	3 cut(s)	384, 772, 3498
EcoT22I	ATGCAT	2 cut(s)	1798, 3543
EcoT38I	GRGCYC	2 cut(s)	3233, 3291
ErhI	CCWWGG	3 cut(s)	384, 772, 3498
Esp3I	CGTCTC	1 cut(s)	35
FalI	AAGNNNNNCTT	4 cut(s)	113, 145, 2963, 2995
FaqI	GGGAC	3 cut(s)	1576, 2520, 2526
FauI	CCCGC	1 cut(s)	3253
FblI	GTMKAC	3 cut(s)	2154, 2796, 3327
Fnu4HI	GCNGC	6 cut(s)	9, 267, 1178, 1417, 1646, 2269
FokI	GGATG	4 cut(s)	907, 1794, 2649, 3357
FriOI	GRGCYC	2 cut(s)	3233, 3291
Fsp4HI	GCNGC	6 cut(s)	9, 267, 1178, 1417, 1646, 2269
FspBI	CTAG	7 cut(s)	623, 674, 1526, 2087, 2862, 3176, 3431
GlaI	GCGC	2 cut(s)	953, 3508
GluI	GCNGC	6 cut(s)	9, 267, 1178, 1417, 1646, 2269
GsuI	CTGGAG	3 cut(s)	1656, 2342, 3267
HaeII	RGCGCY	1 cut(s)	955
HapII	CCGG	4 cut(s)	1779, 2355, 3521, 3577
HgaI	GACGC	2 cut(s)	17, 2557
HhaI	GCGC	2 cut(s)	954, 3509
Hin1I	GRCGYC	1 cut(s)	2568
Hin6I	GCGC	2 cut(s)	952, 3507
HinP1I	GCGC	2 cut(s)	952, 3507
HincII	GTYRAC	6 cut(s)	441, 594, 1856, 1953, 2155, 2178
HindII	GTYRAC	6 cut(s)	441, 594, 1856, 1953, 2155, 2178
HindIII	AAGCTT	6 cut(s)	737, 2026, 2182, 2309, 2462, 2645
HinfI	GANTC	8 cut(s)	626, 1351, 2326, 2687, 2858, 2884, 3236, 3524
HpaI	GTTAAC	2 cut(s)	441, 2178
HpaII	CCGG	4 cut(s)	1779, 2355, 3521, 3577
HpyCH4III	ACNGT	4 cut(s)	229, 1409, 1959, 2295
HpyCH4IV	ACGT	2 cut(s)	1539, 2165
HpyF10VI	GCNNNNNNNGC	5 cut(s)	61, 1135, 1345, 2025, 2317
HpySE526I	ACGT	2 cut(s)	1539, 2165
Hsp92I	GRCGYC	1 cut(s)	2568
HspAI	GCGC	2 cut(s)	952, 3507
KflI	GGGWCCC	1 cut(s)	2534
Kpn2I	TCCGGA	1 cut(s)	3520
KpnI	GGTACC	1 cut(s)	489
KspAI	GTTAAC	2 cut(s)	441, 2178
Kzo9I	GATC	8 cut(s)	604, 965, 1167, 1274, 1321, 2285, 2659, 3178
LmnI	GCTCC	6 cut(s)	445, 943, 1185, 2969, 3286, 3379
Lsp1109I	GCAGC	4 cut(s)	20, 278, 1189, 2280
LweI	GCATC	1 cut(s)	3379
MaeI	CTAG	7 cut(s)	623, 674, 1526, 2087, 2862, 3176, 3431
MaeII	ACGT	2 cut(s)	1539, 2165
MaeIII	GTNAC	6 cut(s)	542, 1835, 2602, 2864, 3307, 3487
MalI	GATC	8 cut(s)	606, 967, 1169, 1276, 1323, 2287, 2661, 3180
MboI	GATC	8 cut(s)	604, 965, 1167, 1274, 1321, 2285, 2659, 3178
MboII	GAAGA	9 cut(s)	129, 133, 524, 863, 975, 1373, 2715, 2787, 2867
MfeI	CAATTG	1 cut(s)	1153
MflI	RGATCY	1 cut(s)	1321
MhlI	GDGCHC	4 cut(s)	1692, 3071, 3233, 3291
MlsI	TGGCCA	2 cut(s)	702, 1947
MluNI	TGGCCA	2 cut(s)	702, 1947
MlyI	GAGTC	1 cut(s)	620
MmeI	TCCRAC	4 cut(s)	594, 2238, 2571, 2790
Mox20I	TGGCCA	2 cut(s)	702, 1947
Mph1103I	ATGCAT	2 cut(s)	1798, 3543
MroI	TCCGGA	1 cut(s)	3520
MroXI	GAANNNNTTC	4 cut(s)	1628, 1700, 1735, 2474
MscI	TGGCCA	2 cut(s)	702, 1947
MslI	CAYNNNNRTG	2 cut(s)	2937, 3074
Msp20I	TGGCCA	2 cut(s)	702, 1947
MspA1I	CMGCKG	1 cut(s)	502
MspCI	CTTAAG	2 cut(s)	2263, 2624
MspI	CCGG	4 cut(s)	1779, 2355, 3521, 3577
MspR9I	CCNGG	5 cut(s)	2160, 2356, 2968, 3375, 3577
MunI	CAATTG	1 cut(s)	1153
Mva1269I	GAATGC	2 cut(s)	1883, 3293
MvaI	CCWGG	3 cut(s)	2160, 2968, 3375
MvnI	CGCG	1 cut(s)	3150
MwoI	GCNNNNNNNGC	5 cut(s)	61, 1135, 1345, 2025, 2317
NciI	CCSGG	2 cut(s)	2356, 3577
NdeII	GATC	8 cut(s)	604, 965, 1167, 1274, 1321, 2285, 2659, 3178
NlaIV	GGNNCC	7 cut(s)	487, 1370, 1564, 2057, 2535, 2536, 3282
NmuCI	GTSAC	4 cut(s)	1835, 2602, 2864, 3307
NsiI	ATGCAT	2 cut(s)	1798, 3543
OliI	CACNNNNGTG	1 cut(s)	3074
PaeR7I	CTCGAG	1 cut(s)	2105
PagI	TCATGA	1 cut(s)	3357
PaqCI	CACCTGC	1 cut(s)	2253
PctI	GAATGC	2 cut(s)	1883, 3293
PdmI	GAANNNNTTC	4 cut(s)	1628, 1700, 1735, 2474
PfeI	GAWTC	7 cut(s)	1351, 2326, 2687, 2858, 2884, 3236, 3524
PflMI	CCANNNNNTGG	3 cut(s)	1220, 1436, 2444
PfoI	TCCNGGA	2 cut(s)	2354, 2966
PkrI	GCNGC	6 cut(s)	10, 268, 1179, 1418, 1647, 2270
PleI	GAGTC	1 cut(s)	620
PpsI	GAGTC	1 cut(s)	620
PpuMI	RGGWCCY	1 cut(s)	2534
PsiI	TTATAA	1 cut(s)	2315
Psp124BI	GAGCTC	2 cut(s)	3233, 3291
Psp1406I	AACGTT	1 cut(s)	2165
Psp5II	RGGWCCY	1 cut(s)	2534
Psp6I	CCWGG	3 cut(s)	2158, 2966, 3373
PspEI	GGTNACC	1 cut(s)	2602
PspGI	CCWGG	3 cut(s)	2158, 2966, 3373
PspN4I	GGNNCC	7 cut(s)	487, 1370, 1564, 2057, 2535, 2536, 3282
PspPPI	RGGWCCY	1 cut(s)	2534
PspXI	VCTCGAGB	1 cut(s)	2105
PstI	CTGCAG	2 cut(s)	268, 2250
PstNI	CAGNNNCTG	2 cut(s)	233, 1413
PsuI	RGATCY	1 cut(s)	1321
PvuII	CAGCTG	1 cut(s)	502
RsaI	GTAC	8 cut(s)	487, 522, 1012, 1256, 2252, 2765, 3057, 3604
RsaNI	GTAC	8 cut(s)	486, 521, 1011, 1255, 2251, 2764, 3056, 3603
RseI	CAYNNNNRTG	2 cut(s)	2937, 3074
SacI	GAGCTC	2 cut(s)	3233, 3291
SalI	GTCGAC	1 cut(s)	2153
SatI	GCNGC	6 cut(s)	9, 267, 1178, 1417, 1646, 2269
Sau3AI	GATC	8 cut(s)	604, 965, 1167, 1274, 1321, 2285, 2659, 3178
ScaI	AGTACT	2 cut(s)	2252, 3057
SchI	GAGTC	1 cut(s)	620
ScrFI	CCNGG	5 cut(s)	2160, 2356, 2968, 3375, 3577
SduI	GDGCHC	4 cut(s)	1692, 3071, 3233, 3291
SfaNI	GCATC	1 cut(s)	3379
SfcI	CTRYAG	5 cut(s)	264, 466, 927, 2246, 3255
Sfr274I	CTCGAG	1 cut(s)	2105
SinI	GGWCC	5 cut(s)	567, 839, 1563, 2429, 2534
SlaI	CTCGAG	1 cut(s)	2105
SmiMI	CAYNNNNRTG	2 cut(s)	2937, 3074
SmlI	CTYRAG	6 cut(s)	938, 1600, 1726, 2105, 2263, 2624
SmoI	CTYRAG	6 cut(s)	938, 1600, 1726, 2105, 2263, 2624
SsiI	CCGC	6 cut(s)	214, 425, 1416, 1646, 3148, 3260
SspI	AATATT	3 cut(s)	527, 1207, 2488
SspMI	CTAG	7 cut(s)	623, 674, 1526, 2087, 2862, 3176, 3431
SstI	GAGCTC	2 cut(s)	3233, 3291
StyD4I	CCNGG	5 cut(s)	2158, 2354, 2966, 3373, 3575
StyI	CCWWGG	3 cut(s)	384, 772, 3498
TaaI	ACNGT	4 cut(s)	229, 1409, 1959, 2295
TaiI	ACGT	2 cut(s)	1542, 2168
TaqI	TCGA	6 cut(s)	67, 288, 2106, 2154, 2658, 3181
TatI	WGTACW	3 cut(s)	1254, 2250, 3055
TauI	GCSGC	2 cut(s)	1419, 1648
TfiI	GAWTC	7 cut(s)	1351, 2326, 2687, 2858, 2884, 3236, 3524
TscAI	CASTG	7 cut(s)	268, 1057, 1204, 1579, 1999, 2743, 3474
TseFI	GTSAC	4 cut(s)	1835, 2602, 2864, 3307
TseI	GCWGC	4 cut(s)	8, 266, 1177, 2268
Tsp45I	GTSAC	4 cut(s)	1835, 2602, 2864, 3307
TspGWI	ACGGA	3 cut(s)	458, 1907, 1923
TspRI	CASTG	7 cut(s)	268, 1057, 1204, 1579, 1999, 2743, 3474
Van91I	CCANNNNNTGG	3 cut(s)	1220, 1436, 2444
Vha464I	CTTAAG	2 cut(s)	2263, 2624
VneI	GTGCAC	1 cut(s)	1688
VpaK11BI	GGWCC	5 cut(s)	567, 839, 1563, 2429, 2534
XcmI	CCANNNNNNNNNTGG	3 cut(s)	388, 3544, 3619
XhoI	CTCGAG	1 cut(s)	2105
XmiI	GTMKAC	3 cut(s)	2154, 2796, 3327
XmnI	GAANNNNTTC	4 cut(s)	1628, 1700, 1735, 2474
XspI	CTAG	7 cut(s)	623, 674, 1526, 2087, 2862, 3176, 3431
ZrmI	AGTACT	2 cut(s)	2252, 3057
Zsp2I	ATGCAT	2 cut(s)	1798, 3543

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.