pycom02g24980

Ubiquitin carboxyl-terminal hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0007263
Physical Location & Seq
Reverse (-)
23038687 .. 23040946
2260 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1161 bp
ATGAGTAGTATAAATTTTGTTACTGCAAAATCTTCGGTTCAATTTAATGGCGAATCAAGCGGACAGAAAAGTTCTTTGAAAGCGCCCACCAAACTTCAGCCGTTAAGTTCACTTAATAAGCAGGATGAAGTTAAGGCTGGAAAAGCAGTAGATGGTAATTTACCTGTATCGTCAGCAACTCCGGCAGAGAATGGATGCACTGATAATTATGTTAATTGTTCTGCCCATAGTAAAGGTGTGAAAGAGGTGACAACTAATGAAGTTAACTTAACCTCTTTGCCTTTATCCAAGGACGAAGGTGGTCTCTCCAATCAATTTTCAAGTCTGGAATTGCAGAATAGAGGGCAGAATGGCAGTGTTGATGATTTGTCTGTTTCTAAGATTAAGGGGGAACTACAGAAGGCTTCAATTGCGCCTGTTACAGTTTCTAAAAGCTTATTGCCTCGTGGTCTAATCAATTCAGGAAATCTATGCTTTCTAAATGCAACCCTGCAGGCTCTTCTATCCTGCTCTCCGTTTGTTCAGCTTCTGCAGGAATTGAGAACTCTTGAAGTACCTAAGGTTGGCTATCCGACATTGAGTGCATTTGCAGAGTTCGTGTCTGAATTTGACATGCCTTGTGGTTCAAGTTCGAAGGACAAAGATACAAGTGTTCTCAAGACTGGTAGGCCTTTTAGCCCTGCCATGTTTGAAGGCGTTCTTAAAAATTTCACTCCAGATGTTCCGACTAGCATCTCCGGCAGGCCAAGACAAGAGGATGCTCAGGAGTTCCTTAGCTTTATCATGGATCAGATACATGATGAATTACTTAAGCTTGAAGGACAATCCCCAAGCATTAACGGGTGCAAATCATCTCTTATATCTTCAGCAGAAGATGATGAATGGGAGAAAGTTGGCCCGAAGAATACATCTGCAGTAACCAGGACACAGAGTTTTGTTCCTTCAGAATTGAGTGATATTTTTGGAGGACAATTGAGAAGTGTGGTGAAGGCAAGAGGTGTGGAAATAAAGCTTCCGCTACTGTTCAGCCATATTTATTGCTCCACCTTGATATTTACCCTGACGCTGTTCGTACCATTGAAGATGCACTTAAGTTGTATTCTGCACCAGAAACGCTTGAGGGGTATCGAACATCAGCAGCTGGGAATGCCGGTATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04139 map04139
Pfam Domains
Protein Families
UCH

Protein Analysis

387

Amino Acids

41.73

Weight (kDa)

6.61

Isoelectric Point (pI)

45.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UCH PF00443 149 - 333 2e-17 Ubiquitin carboxyl-terminal hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 60, 1016
AclWI GGATC 1 cut(s) 795
AcsI RAATTY 3 cut(s) 13, 605, 706
AcuI CTGAAG 3 cut(s) 80, 849, 927
AfaI GTAC 2 cut(s) 555, 1074
AfiI CCNNNNNNNGG 1 cut(s) 563
AflII CTTAAG 2 cut(s) 809, 1090
AgsI TTSAA 9 cut(s) 41, 79, 321, 408, 551, 627, 692, 818, 1081
AjnI CCWGG 1 cut(s) 920
AluBI AGCT 6 cut(s) 435, 526, 777, 814, 1012, 1141
AluI AGCT 6 cut(s) 435, 526, 777, 814, 1012, 1141
Alw26I GTCTC 1 cut(s) 308
AlwI GGATC 1 cut(s) 795
AlwNI CAGNNNCTG 2 cut(s) 529, 1141
AoxI GGCC 3 cut(s) 668, 743, 895
ApeKI GCWGC 1 cut(s) 1138
ApoI RAATTY 3 cut(s) 13, 605, 706
Asp700I GAANNNNTTC 1 cut(s) 696
AspLEI GCGC 2 cut(s) 85, 415
AspS9I GGNCC 1 cut(s) 896
AsuHPI GGTGA 2 cut(s) 259, 997
AsuII TTCGAA 1 cut(s) 632
AxyI CCTNAGG 1 cut(s) 558
BaeI ACNNNNGTAYC 2 cut(s) 636, 669
BauI CACGAG 1 cut(s) 444
BbvI GCAGC 1 cut(s) 1150
BccI CCATC 1 cut(s) 146
BceAI ACGGC 1 cut(s) 85
BcgI CGANNNNNNTGC 2 cut(s) 15, 49
BciT130I CCWGG 1 cut(s) 922
BcoDI GTCTC 1 cut(s) 308
BfaI CTAG 1 cut(s) 729
BfmI CTRYAG 4 cut(s) 395, 491, 530, 912
BfoI RGCGCY 1 cut(s) 86
BfrI CTTAAG 2 cut(s) 809, 1090
BisI GCNGC 1 cut(s) 1139
BlsI GCNGC 1 cut(s) 1140
Bme1390I CCNGG 1 cut(s) 922
BmgT120I GGNCC 1 cut(s) 896
BmrFI CCNGG 1 cut(s) 922
BmsI GCATC 4 cut(s) 185, 741, 748, 1074
BpmI CTGGAG 1 cut(s) 699
Bpu10I CCTNAGC 2 cut(s) 762, 773
Bpu14I TTCGAA 1 cut(s) 632
BpuEI CTTGAG 2 cut(s) 641, 1138
BsaI GGTCTC 1 cut(s) 308
BsaJI CCNNGG 1 cut(s) 288
Bsc4I CCNNNNNNNGG 1 cut(s) 563
Bse118I RCCGGY 1 cut(s) 1150
Bse1I ACTGG 1 cut(s) 667
Bse21I CCTNAGG 1 cut(s) 558
BseBI CCWGG 1 cut(s) 922
BseDI CCNNGG 1 cut(s) 288
BseGI GGATG 3 cut(s) 130, 200, 763
BseLI CCNNNNNNNGG 1 cut(s) 563
BseMII CTCAG 1 cut(s) 776
BseNI ACTGG 1 cut(s) 667
BseXI GCAGC 1 cut(s) 1150
BseYI CCCAGC 1 cut(s) 1141
BsgI GTGCAG 1 cut(s) 1088
BshFI GGCC 3 cut(s) 670, 745, 897
BsiSI CCGG 3 cut(s) 182, 738, 1151
BslI CCNNNNNNNGG 1 cut(s) 563
BsmAI GTCTC 1 cut(s) 308
BsmI GAATGC 1 cut(s) 1152
BsnI GGCC 3 cut(s) 670, 745, 897
Bso31I GGTCTC 1 cut(s) 308
Bsp119I TTCGAA 1 cut(s) 632
Bsp143I GATC 1 cut(s) 787
BspACI CCGC 2 cut(s) 60, 1016
BspANI GGCC 3 cut(s) 670, 745, 897
BspCNI CTCAG 1 cut(s) 775
BspMAI CTGCAG 3 cut(s) 495, 534, 916
BspPI GGATC 1 cut(s) 795
BspQI GCTCTTC 1 cut(s) 504
BspT104I TTCGAA 1 cut(s) 632
BspTI CTTAAG 2 cut(s) 809, 1090
BspTNI GGTCTC 1 cut(s) 308
BsrFI RCCGGY 1 cut(s) 1150
BsrI ACTGG 1 cut(s) 667
BssAI RCCGGY 1 cut(s) 1150
BssECI CCNNGG 1 cut(s) 288
BssMI GATC 1 cut(s) 787
BssSI CACGAG 1 cut(s) 444
BssT1I CCWWGG 1 cut(s) 288
Bst2BI CACGAG 1 cut(s) 444
Bst2UI CCWGG 1 cut(s) 922
Bst4CI ACNGT 2 cut(s) 424, 1023
Bst6I CTCTTC 1 cut(s) 504
BstAFI CTTAAG 2 cut(s) 809, 1090
BstBI TTCGAA 1 cut(s) 632
BstC8I GCNNGC 2 cut(s) 495, 743
BstDEI CTNAG 4 cut(s) 378, 558, 762, 773
BstF5I GGATG 3 cut(s) 130, 200, 763
BstH2I RGCGCY 1 cut(s) 86
BstHHI GCGC 2 cut(s) 85, 415
BstKTI GATC 1 cut(s) 790
BstMAI GTCTC 1 cut(s) 308
BstMBI GATC 1 cut(s) 787
BstMWI GCNNNNNNNGC 6 cut(s) 57, 143, 182, 410, 738, 1147
BstNI CCWGG 1 cut(s) 922
BstNSI RCATGY 1 cut(s) 616
BstSCI CCNGG 1 cut(s) 920
BstSFI CTRYAG 4 cut(s) 395, 491, 530, 912
BstV1I GCAGC 1 cut(s) 1150
Bsu36I CCTNAGG 1 cut(s) 558
BsuRI GGCC 3 cut(s) 670, 745, 897
BtsCI GGATG 3 cut(s) 130, 200, 763
BtsI GCAGTG 1 cut(s) 361
BtsIMutI CAGTG 2 cut(s) 198, 361
Cac8I GCNNGC 2 cut(s) 495, 743
CaiI CAGNNNCTG 2 cut(s) 529, 1141
CfoI GCGC 2 cut(s) 85, 415
Cfr10I RCCGGY 1 cut(s) 1150
Cfr13I GGNCC 1 cut(s) 896
CseI GACGC 1 cut(s) 1072
Csp6I GTAC 2 cut(s) 554, 1073
CspCI CAANNNNNGTGG 2 cut(s) 981, 1016
CviAII CATG 4 cut(s) 613, 685, 784, 797
CviQI GTAC 2 cut(s) 554, 1073
DdeI CTNAG 4 cut(s) 378, 558, 762, 773
DpnI GATC 1 cut(s) 789
DpnII GATC 1 cut(s) 787
Eam1104I CTCTTC 1 cut(s) 504
EarI CTCTTC 1 cut(s) 504
Eco130I CCWWGG 1 cut(s) 288
Eco147I AGGCCT 1 cut(s) 670
Eco31I GGTCTC 1 cut(s) 308
Eco57I CTGAAG 3 cut(s) 80, 849, 927
Eco81I CCTNAGG 1 cut(s) 558
EcoRII CCWGG 1 cut(s) 920
EcoT14I CCWWGG 1 cut(s) 288
ErhI CCWWGG 1 cut(s) 288
FaeI CATG 4 cut(s) 616, 688, 787, 800
FalI AAGNNNNNCTT 4 cut(s) 684, 716, 1073, 1105
FatI CATG 4 cut(s) 612, 684, 783, 796
Fnu4HI GCNGC 1 cut(s) 1139
FokI GGATG 3 cut(s) 137, 207, 770
Fsp4HI GCNGC 1 cut(s) 1139
FspBI CTAG 1 cut(s) 729
GlaI GCGC 2 cut(s) 84, 414
GluI GCNGC 1 cut(s) 1139
GsaI CCCAGC 1 cut(s) 1145
GsuI CTGGAG 1 cut(s) 699
HaeII RGCGCY 1 cut(s) 86
HaeIII GGCC 3 cut(s) 670, 745, 897
HapII CCGG 3 cut(s) 182, 738, 1151
HgaI GACGC 1 cut(s) 1072
HhaI GCGC 2 cut(s) 85, 415
Hin1II CATG 4 cut(s) 616, 688, 787, 800
Hin6I GCGC 2 cut(s) 83, 413
HinP1I GCGC 2 cut(s) 83, 413
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HindIII AAGCTT 3 cut(s) 433, 812, 1010
HinfI GANTC 1 cut(s) 53
HpaI GTTAAC 1 cut(s) 265
HpaII CCGG 3 cut(s) 182, 738, 1151
HphI GGTGA 2 cut(s) 259, 997
Hpy166II GTNNAC 2 cut(s) 110, 265
Hpy188I TCNGA 5 cut(s) 573, 604, 726, 792, 946
Hpy188III TCNNGA 6 cut(s) 326, 462, 548, 658, 716, 764
Hpy8I GTNNAC 2 cut(s) 110, 265
HpyAV CCTTC 7 cut(s) 290, 394, 628, 686, 812, 951, 982
HpyCH4III ACNGT 2 cut(s) 424, 1023
HpyF10VI GCNNNNNNNGC 6 cut(s) 57, 143, 182, 410, 738, 1147
HpyF3I CTNAG 4 cut(s) 378, 558, 762, 773
Hsp92II CATG 4 cut(s) 616, 688, 787, 800
HspAI GCGC 2 cut(s) 83, 413
KspAI GTTAAC 1 cut(s) 265
Kzo9I GATC 1 cut(s) 787
LguI GCTCTTC 1 cut(s) 504
LmnI GCTCC 1 cut(s) 1046
Lsp1109I GCAGC 1 cut(s) 1150
LweI GCATC 4 cut(s) 185, 741, 748, 1074
MaeI CTAG 1 cut(s) 729
MaeIII GTNAC 4 cut(s) 19, 247, 418, 916
MalI GATC 1 cut(s) 789
MboI GATC 1 cut(s) 787
MboII GAAGA 6 cut(s) 24, 491, 855, 884, 913, 1093
MfeI CAATTG 2 cut(s) 408, 971
MmeI TCCRAC 2 cut(s) 596, 749
MnlI CCTC 8 cut(s) 238, 283, 335, 453, 748, 959, 989, 1113
MroXI GAANNNNTTC 1 cut(s) 696
MspA1I CMGCKG 1 cut(s) 1141
MspCI CTTAAG 2 cut(s) 809, 1090
MspI CCGG 3 cut(s) 182, 738, 1151
MspR9I CCNGG 1 cut(s) 922
MunI CAATTG 2 cut(s) 408, 971
Mva1269I GAATGC 1 cut(s) 1152
MvaI CCWGG 1 cut(s) 922
MwoI GCNNNNNNNGC 6 cut(s) 57, 143, 182, 410, 738, 1147
NdeII GATC 1 cut(s) 787
NlaIII CATG 4 cut(s) 616, 688, 787, 800
NmuCI GTSAC 1 cut(s) 247
NspI RCATGY 1 cut(s) 616
NspV TTCGAA 1 cut(s) 632
PceI AGGCCT 1 cut(s) 670
PciSI GCTCTTC 1 cut(s) 504
PctI GAATGC 1 cut(s) 1152
PdmI GAANNNNTTC 1 cut(s) 696
PfeI GAWTC 1 cut(s) 53
PkrI GCNGC 1 cut(s) 1140
Psp6I CCWGG 1 cut(s) 920
PspFI CCCAGC 1 cut(s) 1141
PspGI CCWGG 1 cut(s) 920
PspPI GGNCC 1 cut(s) 896
PstI CTGCAG 3 cut(s) 495, 534, 916
PstNI CAGNNNCTG 2 cut(s) 529, 1141
PvuII CAGCTG 1 cut(s) 1141
RsaI GTAC 2 cut(s) 555, 1074
RsaNI GTAC 2 cut(s) 554, 1073
SapI GCTCTTC 1 cut(s) 504
SatI GCNGC 1 cut(s) 1139
Sau3AI GATC 1 cut(s) 787
Sau96I GGNCC 1 cut(s) 896
SbfI CCTGCAGG 1 cut(s) 495
ScrFI CCNGG 1 cut(s) 922
SdaI CCTGCAGG 1 cut(s) 495
SfaNI GCATC 4 cut(s) 185, 741, 748, 1074
SfcI CTRYAG 4 cut(s) 395, 491, 530, 912
SfuI TTCGAA 1 cut(s) 632
SmlI CTYRAG 4 cut(s) 656, 809, 1090, 1117
SmoI CTYRAG 4 cut(s) 656, 809, 1090, 1117
Sse8387I CCTGCAGG 1 cut(s) 495
SseBI AGGCCT 1 cut(s) 670
SsiI CCGC 2 cut(s) 60, 1016
SspMI CTAG 1 cut(s) 729
StuI AGGCCT 1 cut(s) 670
StyD4I CCNGG 1 cut(s) 920
StyI CCWWGG 1 cut(s) 288
TaaI ACNGT 2 cut(s) 424, 1023
TaqI TCGA 2 cut(s) 632, 1128
TfiI GAWTC 1 cut(s) 53
TscAI CASTG 2 cut(s) 205, 361
TseFI GTSAC 1 cut(s) 247
TseI GCWGC 1 cut(s) 1138
Tsp45I GTSAC 1 cut(s) 247
TspDTI ATGAA 4 cut(s) 141, 273, 816, 894
TspGWI ACGGA 1 cut(s) 504
TspRI CASTG 2 cut(s) 205, 361
Vha464I CTTAAG 2 cut(s) 809, 1090
XapI RAATTY 3 cut(s) 13, 605, 706
XceI RCATGY 1 cut(s) 616
XmnI GAANNNNTTC 1 cut(s) 696
XspI CTAG 1 cut(s) 729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.