pycom02g26060

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0010715
Physical Location & Seq
Reverse (-)
23902573 .. 23906407
3835 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g26060.1

Sequence Viewer

Length: 1053 bp
ATGCAATTGTGTCTGCACCCAAAAGTGAAAGTTAAAATGTTTTCACAAAGCCATTTTCTCTGTGTACTTCTCATAATATTAGTCTCCTTTTCCTTCTCTTTTAGTAGTGACCTCGATAACCCACTAGTTGTGCATGATCTTCATCGTCACCATCGAATACGCCGGCTTCAATCAACTTCAGCAATGGATGTTACTGTAAATGTGGATAGCTTTGGAGCAAAAGGAGATGGATCGGATGATTCCCAGGCATTTACGGAAGCTTGGAAGAAGGCTTGTTCTTCTAAAAGTGATGTTGAGCTCGTGGTCCCTAGGAATAGGGTCTATCAACTGAAGCCAATGGACTTCTCTGGCCCTTGCAAATCTCAGGTCACGCTTAAGATATATGGAACCATCGAAGCCTCTACCAAACAATCAGATTATAAGGACGGCCGGCATTGGATTCTGTTTGCAAATTTGACTAACTTTAGAGTCGAAGGTGGTGGCACCATCGATGGCAATGGAAGGAAATGGTGGCAAAGCTCATGCAAAGTGGACGAATCCCGTCATTGCACAACCGCACCAAATGCTGTGACTTTCTTGGGGTGCAACACTTTGGAAGTTGATAATTTGAGGTTCCAAGATGCCCAAAAAATGCATCTATCTTTTGAGTCGTGTGTGAATGTAAAAGCTTCGAATCTCATAGTGACCGCACCAGGGGATAGCCCCAACACAGATGGAATCCATGTATCAGCAACACAGGATATTGGGATTAACAACTGTCTCATTAAGACAGGTGATGACTGTATTTCGATAGTAAGTGGGTCAAAAAATGTTCGAGCTGAGGATATAACATGTGGACCAGGTCATGGAATCAGCATTGGAAGCCTAGGAGCCGGAAATTCACAAGCAGAGGTTTCAGATGTGATGGTTTCTAGAGCCAACCTTATGGACACCACTAATGGATTGCGAATTAAGACTTGGCAGGGCGGGTCTGGATATGCAACAAACATCAGATTCAAAAATGTGGTGATGGCCAACGTGAGCAATCCAATTATCATAGATCAACATTACTGA

Protein Analysis

351

Amino Acids

38.29

Weight (kDa)

7.59

Isoelectric Point (pI)

34.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 92 - 350 7.4e-87 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010715)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07970
fragaria_vesca FvH4_7g01140 FvH4_7g01140
malus_domestica MD02G1309400.v1.1 MD07G1011600.v1.1
prunus_persica Prupe.2G014800_v2.0.a1
pyrus_communis pycom02g26060 pycom07g00970
rosa_chinensis RchiOBHm_Chr1g0316321
rosa_laevigata RLG00000030679
rosa_multiflora Rmu_sc0002531.1_g000005 Rmu_sc0004511.1_g000005
rosa_roxburghii Rroxscaffold_4G00331050
rosa_rugosa Rorug01G0009700 Rorug01G0009700
rosa_samantha Rh1AG019500 Rh1BG014600 Rh1CG017100 Rh1DG014500
rosa_wichuraiana Rw1G001310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 420
AccB1I GGYRCC 1 cut(s) 482
AccB7I CCANNNNNTGG 1 cut(s) 845
AciI CCGC 3 cut(s) 555, 687, 966
AclWI GGATC 1 cut(s) 238
AcoI YGGCCR 2 cut(s) 427, 1011
AcsI RAATTY 2 cut(s) 451, 877
AcuI CTGAAG 2 cut(s) 162, 350
AfaI GTAC 1 cut(s) 66
AfiI CCNNNNNNNGG 2 cut(s) 693, 845
AflII CTTAAG 1 cut(s) 374
AflIII ACRYGT 1 cut(s) 830
AgsI TTSAA 2 cut(s) 170, 997
AhlI ACTAGT 1 cut(s) 124
AjnI CCWGG 3 cut(s) 243, 691, 838
AjuI GAANNNNNNNTTGG 2 cut(s) 940, 972
AluBI AGCT 6 cut(s) 210, 260, 298, 519, 668, 818
AluI AGCT 6 cut(s) 210, 260, 298, 519, 668, 818
Alw21I GWGCWC 1 cut(s) 300
Alw26I GTCTC 2 cut(s) 88, 764
AlwI GGATC 1 cut(s) 238
AoxI GGCC 3 cut(s) 349, 427, 1011
ApoI RAATTY 2 cut(s) 451, 877
ArsI GACNNNNNNTTYG 2 cut(s) 351, 383
AspA2I CCTAGG 2 cut(s) 308, 865
AspS9I GGNCC 3 cut(s) 304, 350, 836
AsuHPI GGTGA 3 cut(s) 140, 785, 1018
AsuII TTCGAA 1 cut(s) 671
AvaII GGWCC 2 cut(s) 304, 836
AvrII CCTAGG 2 cut(s) 308, 865
BalI TGGCCA 1 cut(s) 1013
BanI GGYRCC 1 cut(s) 482
BanII GRGCYC 1 cut(s) 300
BauI CACGAG 1 cut(s) 299
Bbv12I GWGCWC 1 cut(s) 300
BbvCI CCTCAGC 1 cut(s) 819
BccI CCATC 8 cut(s) 159, 221, 398, 485, 494, 707, 898, 1003
BceAI ACGGC 1 cut(s) 442
BciT130I CCWGG 3 cut(s) 245, 693, 840
BcoDI GTCTC 2 cut(s) 88, 764
BcuI ACTAGT 1 cut(s) 124
BfaI CTAG 4 cut(s) 125, 309, 866, 912
BfrI CTTAAG 1 cut(s) 374
BlnI CCTAGG 2 cut(s) 308, 865
Bme1390I CCNGG 3 cut(s) 245, 693, 840
Bme18I GGWCC 2 cut(s) 304, 836
BmgT120I GGNCC 3 cut(s) 304, 350, 836
BmiI GGNNCC 5 cut(s) 306, 388, 484, 614, 871
BmrFI CCNGG 3 cut(s) 245, 693, 840
BmsI GCATC 2 cut(s) 610, 643
Bpu10I CCTNAGC 1 cut(s) 819
Bpu14I TTCGAA 1 cut(s) 671
Bsa29I ATCGAT 1 cut(s) 489
BsaBI GATNNNNATC 1 cut(s) 141
BsaJI CCNNGG 4 cut(s) 243, 308, 692, 865
Bsc4I CCNNNNNNNGG 2 cut(s) 693, 845
Bse118I RCCGGY 2 cut(s) 162, 429
Bse3DI GCAATG 3 cut(s) 189, 502, 544
Bse8I GATNNNNATC 1 cut(s) 141
BseBI CCWGG 3 cut(s) 245, 693, 840
BseCI ATCGAT 1 cut(s) 489
BseDI CCNNGG 4 cut(s) 243, 308, 692, 865
BseGI GGATG 2 cut(s) 193, 241
BseJI GATNNNNATC 1 cut(s) 141
BseLI CCNNNNNNNGG 2 cut(s) 693, 845
BseMI GCAATG 3 cut(s) 189, 502, 544
BseMII CTCAG 2 cut(s) 377, 810
BseX3I CGGCCG 1 cut(s) 427
Bsh1285I CGRYCG 1 cut(s) 430
BshFI GGCC 3 cut(s) 351, 429, 1013
BshNI GGYRCC 1 cut(s) 482
BshVI ATCGAT 1 cut(s) 489
BsiEI CGRYCG 1 cut(s) 430
BsiHKAI GWGCWC 1 cut(s) 300
BsiSI CCGG 3 cut(s) 163, 430, 873
BslFI GGGAC 1 cut(s) 290
BslI CCNNNNNNNGG 2 cut(s) 693, 845
BsmAI GTCTC 2 cut(s) 88, 764
BsmFI GGGAC 1 cut(s) 290
BsnI GGCC 3 cut(s) 351, 429, 1013
Bsp119I TTCGAA 1 cut(s) 671
Bsp1286I GDGCHC 1 cut(s) 300
Bsp143I GATC 3 cut(s) 136, 230, 1039
BspACI CCGC 3 cut(s) 555, 687, 966
BspANI GGCC 3 cut(s) 351, 429, 1013
BspCNI CTCAG 2 cut(s) 376, 811
BspDI ATCGAT 1 cut(s) 489
BspLI GGNNCC 5 cut(s) 306, 388, 484, 614, 871
BspPI GGATC 1 cut(s) 238
BspT104I TTCGAA 1 cut(s) 671
BspT107I GGYRCC 1 cut(s) 482
BspTI CTTAAG 1 cut(s) 374
BsrDI GCAATG 3 cut(s) 189, 502, 544
BsrFI RCCGGY 2 cut(s) 162, 429
BssAI RCCGGY 2 cut(s) 162, 429
BssECI CCNNGG 4 cut(s) 243, 308, 692, 865
BssMI GATC 3 cut(s) 136, 230, 1039
BssSI CACGAG 1 cut(s) 299
BssT1I CCWWGG 2 cut(s) 308, 865
Bst2BI CACGAG 1 cut(s) 299
Bst2UI CCWGG 3 cut(s) 245, 693, 840
Bst4CI ACNGT 3 cut(s) 196, 758, 782
BstAFI CTTAAG 1 cut(s) 374
BstAPI GCANNNNNTGC 1 cut(s) 563
BstBI TTCGAA 1 cut(s) 671
BstC8I GCNNGC 2 cut(s) 164, 431
BstDEI CTNAG 2 cut(s) 363, 819
BstF5I GGATG 2 cut(s) 193, 241
BstKTI GATC 3 cut(s) 139, 233, 1042
BstMAI GTCTC 2 cut(s) 88, 764
BstMBI GATC 3 cut(s) 136, 230, 1039
BstMCI CGRYCG 1 cut(s) 430
BstMWI GCNNNNNNNGC 2 cut(s) 563, 861
BstNI CCWGG 3 cut(s) 245, 693, 840
BstNSI RCATGY 1 cut(s) 834
BstSCI CCNGG 3 cut(s) 243, 691, 838
BstXI CCANNNNNNTGG 1 cut(s) 925
BstZI CGGCCG 1 cut(s) 427
Bsu15I ATCGAT 1 cut(s) 489
BsuRI GGCC 3 cut(s) 351, 429, 1013
BsuTUI ATCGAT 1 cut(s) 489
BtsCI GGATG 2 cut(s) 193, 241
Cac8I GCNNGC 2 cut(s) 164, 431
Cfr10I RCCGGY 2 cut(s) 162, 429
Cfr13I GGNCC 3 cut(s) 304, 350, 836
ClaI ATCGAT 1 cut(s) 489
CsiI ACCWGGT 1 cut(s) 838
Csp6I GTAC 1 cut(s) 65
CviAII CATG 5 cut(s) 134, 522, 722, 831, 845
CviQI GTAC 1 cut(s) 65
DdeI CTNAG 2 cut(s) 363, 819
DpnI GATC 3 cut(s) 138, 232, 1041
DpnII GATC 3 cut(s) 136, 230, 1039
EaeI YGGCCR 2 cut(s) 427, 1011
EagI CGGCCG 1 cut(s) 427
Ecl136II GAGCTC 1 cut(s) 298
EclXI CGGCCG 1 cut(s) 427
Eco130I CCWWGG 2 cut(s) 308, 865
Eco24I GRGCYC 1 cut(s) 300
Eco47I GGWCC 2 cut(s) 304, 836
Eco52I CGGCCG 1 cut(s) 427
Eco53kI GAGCTC 1 cut(s) 298
Eco57I CTGAAG 2 cut(s) 162, 350
EcoICRI GAGCTC 1 cut(s) 298
EcoRII CCWGG 3 cut(s) 243, 691, 838
EcoT14I CCWWGG 2 cut(s) 308, 865
EcoT22I ATGCAT 1 cut(s) 636
EcoT38I GRGCYC 1 cut(s) 300
ErhI CCWWGG 2 cut(s) 308, 865
FaeI CATG 5 cut(s) 137, 525, 725, 834, 848
FaqI GGGAC 1 cut(s) 290
FatI CATG 5 cut(s) 133, 521, 721, 830, 844
FauI CCCGC 1 cut(s) 959
FokI GGATG 2 cut(s) 200, 248
FriOI GRGCYC 1 cut(s) 300
FspBI CTAG 4 cut(s) 125, 309, 866, 912
HaeIII GGCC 3 cut(s) 351, 429, 1013
HapII CCGG 3 cut(s) 163, 430, 873
Hin1II CATG 5 cut(s) 137, 525, 725, 834, 848
HindIII AAGCTT 2 cut(s) 258, 666
HinfI GANTC 9 cut(s) 239, 439, 468, 536, 647, 673, 717, 849, 993
HpaII CCGG 3 cut(s) 163, 430, 873
HphI GGTGA 3 cut(s) 140, 785, 1018
Hpy166II GTNNAC 3 cut(s) 65, 532, 836
Hpy188I TCNGA 4 cut(s) 235, 415, 898, 992
Hpy188III TCNNGA 2 cut(s) 912, 972
Hpy8I GTNNAC 3 cut(s) 65, 532, 836
HpyAV CCTTC 4 cut(s) 103, 262, 467, 495
HpyCH4III ACNGT 3 cut(s) 196, 758, 782
HpyCH4IV ACGT 1 cut(s) 1017
HpyF10VI GCNNNNNNNGC 2 cut(s) 563, 861
HpyF3I CTNAG 2 cut(s) 363, 819
HpySE526I ACGT 1 cut(s) 1017
Hsp92II CATG 5 cut(s) 137, 525, 725, 834, 848
KroI GCCGGC 2 cut(s) 162, 429
KroNI GCCGGC 2 cut(s) 164, 431
Kzo9I GATC 3 cut(s) 136, 230, 1039
LmnI GCTCC 2 cut(s) 215, 869
LweI GCATC 2 cut(s) 610, 643
MabI ACCWGGT 1 cut(s) 838
MaeI CTAG 4 cut(s) 125, 309, 866, 912
MaeII ACGT 1 cut(s) 1017
MaeIII GTNAC 6 cut(s) 107, 146, 190, 367, 568, 682
MalI GATC 3 cut(s) 138, 232, 1041
MboI GATC 3 cut(s) 136, 230, 1039
MboII GAAGA 3 cut(s) 131, 270, 277
MfeI CAATTG 1 cut(s) 5
MhlI GDGCHC 1 cut(s) 300
MlsI TGGCCA 1 cut(s) 1013
MluCI AATT 6 cut(s) 5, 451, 604, 877, 948, 1029
MluNI TGGCCA 1 cut(s) 1013
MlyI GAGTC 2 cut(s) 477, 656
MnlI CCTC 5 cut(s) 122, 409, 603, 814, 883
Mox20I TGGCCA 1 cut(s) 1013
Mph1103I ATGCAT 1 cut(s) 636
MroNI GCCGGC 2 cut(s) 162, 429
MscI TGGCCA 1 cut(s) 1013
MseI TTAA 5 cut(s) 33, 375, 750, 765, 951
Msp20I TGGCCA 1 cut(s) 1013
MspCI CTTAAG 1 cut(s) 374
MspI CCGG 3 cut(s) 163, 430, 873
MspR9I CCNGG 3 cut(s) 245, 693, 840
MunI CAATTG 1 cut(s) 5
MvaI CCWGG 3 cut(s) 245, 693, 840
MwoI GCNNNNNNNGC 2 cut(s) 563, 861
NaeI GCCGGC 2 cut(s) 164, 431
NdeII GATC 3 cut(s) 136, 230, 1039
NgoMIV GCCGGC 2 cut(s) 162, 429
NlaIII CATG 5 cut(s) 137, 525, 725, 834, 848
NlaIV GGNNCC 5 cut(s) 306, 388, 484, 614, 871
NmuCI GTSAC 5 cut(s) 107, 146, 367, 568, 682
NsiI ATGCAT 1 cut(s) 636
NspI RCATGY 1 cut(s) 834
NspV TTCGAA 1 cut(s) 671
PciI ACATGT 1 cut(s) 830
PcsI WCGNNNNNNNCGW 1 cut(s) 151
PdiI GCCGGC 2 cut(s) 164, 431
PfeI GAWTC 7 cut(s) 239, 439, 536, 673, 717, 849, 993
PflFI GACNNNGTC 1 cut(s) 840
PflMI CCANNNNNTGG 1 cut(s) 845
PleI GAGTC 2 cut(s) 476, 655
PpsI GAGTC 2 cut(s) 476, 655
PscI ACATGT 1 cut(s) 830
PsiI TTATAA 1 cut(s) 420
Psp124BI GAGCTC 1 cut(s) 300
Psp6I CCWGG 3 cut(s) 243, 691, 838
PspGI CCWGG 3 cut(s) 243, 691, 838
PspN4I GGNNCC 5 cut(s) 306, 388, 484, 614, 871
PspPI GGNCC 3 cut(s) 304, 350, 836
PsyI GACNNNGTC 1 cut(s) 840
RsaI GTAC 1 cut(s) 66
RsaNI GTAC 1 cut(s) 65
SacI GAGCTC 1 cut(s) 300
SaqAI TTAA 5 cut(s) 33, 375, 750, 765, 951
Sau3AI GATC 3 cut(s) 136, 230, 1039
Sau96I GGNCC 3 cut(s) 304, 350, 836
SchI GAGTC 2 cut(s) 477, 656
ScrFI CCNGG 3 cut(s) 245, 693, 840
SduI GDGCHC 1 cut(s) 300
SexAI ACCWGGT 1 cut(s) 838
SfaNI GCATC 2 cut(s) 610, 643
SfuI TTCGAA 1 cut(s) 671
SinI GGWCC 2 cut(s) 304, 836
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
SpeI ACTAGT 1 cut(s) 124
Sse9I AATT 6 cut(s) 5, 451, 604, 877, 948, 1029
SsiI CCGC 3 cut(s) 555, 687, 966
SspI AATATT 1 cut(s) 78
SspMI CTAG 4 cut(s) 125, 309, 866, 912
SstI GAGCTC 1 cut(s) 300
StyD4I CCNGG 3 cut(s) 243, 691, 838
StyI CCWWGG 2 cut(s) 308, 865
TaaI ACNGT 3 cut(s) 196, 758, 782
TaiI ACGT 1 cut(s) 1020
TaqI TCGA 8 cut(s) 114, 154, 393, 471, 489, 671, 788, 814
TasI AATT 6 cut(s) 5, 451, 604, 877, 948, 1029
TatI WGTACW 1 cut(s) 64
TfiI GAWTC 7 cut(s) 239, 439, 536, 673, 717, 849, 993
Tru1I TTAA 5 cut(s) 33, 375, 750, 765, 951
Tru9I TTAA 5 cut(s) 33, 375, 750, 765, 951
TseFI GTSAC 5 cut(s) 107, 146, 367, 568, 682
Tsp45I GTSAC 5 cut(s) 107, 146, 367, 568, 682
TspDTI ATGAA 1 cut(s) 131
TspGWI ACGGA 1 cut(s) 269
Tth111I GACNNNGTC 1 cut(s) 840
Van91I CCANNNNNTGG 1 cut(s) 845
Vha464I CTTAAG 1 cut(s) 374
VpaK11BI GGWCC 2 cut(s) 304, 836
XapI RAATTY 2 cut(s) 451, 877
XbaI TCTAGA 1 cut(s) 911
XceI RCATGY 1 cut(s) 834
XmaJI CCTAGG 2 cut(s) 308, 865
XspI CTAG 4 cut(s) 125, 309, 866, 912
Zsp2I ATGCAT 1 cut(s) 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.