pycom02g26340

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0006829
Physical Location & Seq
Forward (+)
24159273 .. 24160254
982 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g26340.2

Sequence Viewer

Length: 939 bp
ATGGTCGCCATGATCACACGCAGATTCAGCTCCAAGCTTTCAAGACTCTATCATCATCATCATTCATCATTGTCTGCATCCTCCACCTACCTCGCCGCCCAAAACCTCCAAAAGCCTAAAACCCCAGACCATCTCACTCACTCCTCCAATTCCACTCTCTCCCCAAATCCTTTCAAATCTTCATCAAACCCCACCACCATTATCGATCATTACATAAAATCATCAAATCCAAAACCCATAAACCATAATCTAACTTGGGTACCCAATTCAAAATCCATTACTCAAAGCCCTGAATTCAGAAAACCCAGTACCAATTTGTACAATCCTAGGTTTAATGGGTTATTAGATCAAAAACCCAGATTTTTGTCAACTTCTTCTAGTTCACCGTCACCGGATCCAGAATCTGAAAAGCCCCAAAACCCAAATGAATACCCTAGTCAAAACCCTAACTTCAAGCACCAAGAAATCGAAGGACCGACTGTGGAGCGAGACCCTTCGACTCTGGCCAATGAGACCAGAGAAGTTCTCGAATCGATGGCGAAAAACATGTGTAGTCTGAGCAGGGTATTGGCTCTTCTGGGTTTGGCCCAACTCGGCGTCGGAGCTTGGATTTCATACATCACTAAATCGACCCCAATGCCGGAGGTTTCAGTTCAGAGCATTTTGGCTTTCGGGTTGCCCTTTACGCTGGCGTTCATGTTGAGGCAGAGTTTGAAGCCAATCTACTTCTTTAAGAAGATGGAGGAGCAAGGCAGGCTGTGTTCTTCGTTCGTGTTCGCGGCGTTTCATTCTTGTGCATTGCTGGCTTGTCAGTCGGAGTGTTGTTCGCCGCGCTTACGAGATGAGTTTGTAACTCCCGTTGATGAGATTGTTATGTTGAAGTTTGATTGTTGGATTGTTATAATGTGTAACTTGTGCGGGAAGAAAAGATTAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

313

Amino Acids

35.07

Weight (kDa)

9.11

Isoelectric Point (pI)

52.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 902
Acc65I GGTACC 1 cut(s) 259
AccB1I GGYRCC 1 cut(s) 259
AccII CGCG 2 cut(s) 779, 832
AciI CCGC 4 cut(s) 96, 779, 830, 918
AclWI GGATC 2 cut(s) 389, 402
AcoI YGGCCR 1 cut(s) 504
AcsI RAATTY 1 cut(s) 293
AcyI GRCGYC 1 cut(s) 597
AfaI GTAC 3 cut(s) 261, 310, 320
AfiI CCNNNNNNNGG 1 cut(s) 640
AflIII ACRYGT 1 cut(s) 546
AgsI TTSAA 6 cut(s) 42, 175, 270, 454, 715, 880
AluBI AGCT 3 cut(s) 30, 37, 605
AluI AGCT 3 cut(s) 30, 37, 605
Alw26I GTCTC 2 cut(s) 483, 506
AlwI GGATC 2 cut(s) 389, 402
AlwNI CAGNNNCTG 1 cut(s) 404
AoxI GGCC 2 cut(s) 504, 585
ApoI RAATTY 1 cut(s) 293
AseI ATTAAT 1 cut(s) 932
Asp718I GGTACC 1 cut(s) 259
AspA2I CCTAGG 1 cut(s) 326
AspLEI GCGC 1 cut(s) 834
AspS9I GGNCC 2 cut(s) 473, 586
AsuHPI GGTGA 2 cut(s) 375, 381
AvaII GGWCC 1 cut(s) 473
AvrII CCTAGG 1 cut(s) 326
BaeI ACNNNNGTAYC 2 cut(s) 243, 276
BalI TGGCCA 1 cut(s) 506
BamHI GGATCC 1 cut(s) 394
BanI GGYRCC 1 cut(s) 259
BarI GAAGNNNNNNTAC 4 cut(s) 558, 590, 707, 739
BccI CCATC 3 cut(s) 138, 529, 733
BcgI CGANNNNNNTGC 2 cut(s) 619, 653
BclI TGATCA 1 cut(s) 12
BcoDI GTCTC 2 cut(s) 483, 506
BfaI CTAG 3 cut(s) 327, 378, 435
BisI GCNGC 3 cut(s) 96, 780, 830
BlnI CCTAGG 1 cut(s) 326
BlsI GCNGC 3 cut(s) 97, 781, 831
Bme18I GGWCC 1 cut(s) 473
BmgT120I GGNCC 2 cut(s) 473, 586
BmiI GGNNCC 2 cut(s) 261, 396
BmrI ACTGGG 1 cut(s) 300
BmsI GCATC 1 cut(s) 86
BmuI ACTGGG 1 cut(s) 300
BplI GAGNNNNNCTC 2 cut(s) 510, 542
Bsa29I ATCGAT 2 cut(s) 204, 533
BsaHI GRCGYC 1 cut(s) 597
BsaI GGTCTC 2 cut(s) 483, 506
BsaJI CCNNGG 1 cut(s) 326
BsaWI WCCGGW 1 cut(s) 391
Bsc4I CCNNNNNNNGG 1 cut(s) 640
Bse1I ACTGG 1 cut(s) 306
Bse3DI GCAATG 1 cut(s) 797
BseCI ATCGAT 2 cut(s) 204, 533
BseDI CCNNGG 1 cut(s) 326
BseGI GGATG 1 cut(s) 77
BseLI CCNNNNNNNGG 1 cut(s) 640
BseMI GCAATG 1 cut(s) 797
BseMII CTCAG 1 cut(s) 548
BseNI ACTGG 1 cut(s) 306
BseRI GAGGAG 2 cut(s) 133, 758
Bsh1236I CGCG 2 cut(s) 779, 832
BshFI GGCC 2 cut(s) 506, 587
BshNI GGYRCC 1 cut(s) 259
BshVI ATCGAT 2 cut(s) 204, 533
BsiSI CCGG 2 cut(s) 392, 641
BslI CCNNNNNNNGG 1 cut(s) 640
BsmAI GTCTC 2 cut(s) 483, 506
BsnI GGCC 2 cut(s) 506, 587
Bso31I GGTCTC 2 cut(s) 483, 506
Bsp1407I TGTACA 1 cut(s) 318
Bsp143I GATC 4 cut(s) 12, 205, 346, 394
BspACI CCGC 4 cut(s) 96, 779, 830, 918
BspANI GGCC 2 cut(s) 506, 587
BspCNI CTCAG 1 cut(s) 549
BspDI ATCGAT 2 cut(s) 204, 533
BspFNI CGCG 2 cut(s) 779, 832
BspLI GGNNCC 2 cut(s) 261, 396
BspPI GGATC 2 cut(s) 389, 402
BspQI GCTCTTC 1 cut(s) 579
BspT107I GGYRCC 1 cut(s) 259
BspTNI GGTCTC 2 cut(s) 483, 506
BsrDI GCAATG 1 cut(s) 797
BsrGI TGTACA 1 cut(s) 318
BsrI ACTGG 1 cut(s) 306
BssECI CCNNGG 1 cut(s) 326
BssMI GATC 4 cut(s) 12, 205, 346, 394
BssNI GRCGYC 1 cut(s) 597
BssT1I CCWWGG 1 cut(s) 326
Bst4CI ACNGT 2 cut(s) 387, 481
Bst6I CTCTTC 1 cut(s) 579
BstACI GRCGYC 1 cut(s) 597
BstAUI TGTACA 1 cut(s) 318
BstC8I GCNNGC 3 cut(s) 690, 755, 804
BstDEI CTNAG 1 cut(s) 557
BstF5I GGATG 1 cut(s) 77
BstFNI CGCG 2 cut(s) 779, 832
BstHHI GCGC 1 cut(s) 834
BstKTI GATC 4 cut(s) 15, 208, 349, 397
BstMAI GTCTC 2 cut(s) 483, 506
BstMBI GATC 4 cut(s) 12, 205, 346, 394
BstMWI GCNNNNNNNGC 4 cut(s) 27, 685, 754, 803
BstNSI RCATGY 1 cut(s) 550
BstUI CGCG 2 cut(s) 779, 832
BstX2I RGATCY 1 cut(s) 394
BstYI RGATCY 1 cut(s) 394
Bsu15I ATCGAT 2 cut(s) 204, 533
BsuRI GGCC 2 cut(s) 506, 587
BsuTUI ATCGAT 2 cut(s) 204, 533
BtsCI GGATG 1 cut(s) 77
Cac8I GCNNGC 3 cut(s) 690, 755, 804
CaiI CAGNNNCTG 1 cut(s) 404
CfoI GCGC 1 cut(s) 834
Cfr13I GGNCC 2 cut(s) 473, 586
ClaI ATCGAT 2 cut(s) 204, 533
CseI GACGC 1 cut(s) 586
Csp6I GTAC 3 cut(s) 260, 309, 319
CviAII CATG 3 cut(s) 10, 547, 697
CviQI GTAC 3 cut(s) 260, 309, 319
DdeI CTNAG 1 cut(s) 557
DpnI GATC 4 cut(s) 14, 207, 348, 396
DpnII GATC 4 cut(s) 12, 205, 346, 394
EaeI YGGCCR 1 cut(s) 504
Eam1104I CTCTTC 1 cut(s) 579
EarI CTCTTC 1 cut(s) 579
Eco130I CCWWGG 1 cut(s) 326
Eco31I GGTCTC 2 cut(s) 483, 506
Eco47I GGWCC 1 cut(s) 473
EcoRI GAATTC 1 cut(s) 293
EcoT14I CCWWGG 1 cut(s) 326
ErhI CCWWGG 1 cut(s) 326
FaeI CATG 3 cut(s) 13, 550, 700
FaiI YATR 9 cut(s) 11, 215, 239, 246, 548, 616, 698, 875, 902
FatI CATG 3 cut(s) 9, 546, 696
FauI CCCGC 1 cut(s) 911
FbaI TGATCA 1 cut(s) 12
Fnu4HI GCNGC 3 cut(s) 96, 780, 830
FokI GGATG 1 cut(s) 64
Fsp4HI GCNGC 3 cut(s) 96, 780, 830
FspBI CTAG 3 cut(s) 327, 378, 435
GlaI GCGC 1 cut(s) 833
GluI GCNGC 3 cut(s) 96, 780, 830
HaeIII GGCC 2 cut(s) 506, 587
HapII CCGG 2 cut(s) 392, 641
HgaI GACGC 1 cut(s) 586
HhaI GCGC 1 cut(s) 834
Hin1I GRCGYC 1 cut(s) 597
Hin1II CATG 3 cut(s) 13, 550, 700
Hin6I GCGC 1 cut(s) 832
HinP1I GCGC 1 cut(s) 832
HincII GTYRAC 1 cut(s) 369
HindII GTYRAC 1 cut(s) 369
HindIII AAGCTT 1 cut(s) 35
HinfI GANTC 5 cut(s) 24, 45, 401, 499, 530
HpaII CCGG 2 cut(s) 392, 641
HphI GGTGA 2 cut(s) 375, 381
Hpy166II GTNNAC 2 cut(s) 369, 383
Hpy188I TCNGA 6 cut(s) 299, 406, 558, 602, 657, 817
Hpy188III TCNNGA 3 cut(s) 42, 398, 527
Hpy8I GTNNAC 2 cut(s) 369, 383
Hpy99I CGWCG 1 cut(s) 602
HpyAV CCTTC 2 cut(s) 464, 504
HpyCH4III ACNGT 2 cut(s) 387, 481
HpyCH4V TGCA 2 cut(s) 77, 797
HpyF10VI GCNNNNNNNGC 4 cut(s) 27, 685, 754, 803
HpyF3I CTNAG 1 cut(s) 557
Hsp92I GRCGYC 1 cut(s) 597
Hsp92II CATG 3 cut(s) 13, 550, 700
HspAI GCGC 1 cut(s) 832
KpnI GGTACC 1 cut(s) 263
Ksp22I TGATCA 1 cut(s) 12
Kzo9I GATC 4 cut(s) 12, 205, 346, 394
LguI GCTCTTC 1 cut(s) 579
LmnI GCTCC 4 cut(s) 35, 484, 602, 745
LweI GCATC 1 cut(s) 86
MaeI CTAG 3 cut(s) 327, 378, 435
MaeIII GTNAC 3 cut(s) 387, 850, 908
MalI GATC 4 cut(s) 14, 207, 348, 396
MboI GATC 4 cut(s) 12, 205, 346, 394
MboII GAAGA 6 cut(s) 171, 366, 566, 748, 756, 934
MflI RGATCY 1 cut(s) 394
MlsI TGGCCA 1 cut(s) 506
MluCI AATT 4 cut(s) 148, 265, 293, 313
MluNI TGGCCA 1 cut(s) 506
MlyI GAGTC 2 cut(s) 39, 493
MmeI TCCRAC 3 cut(s) 580, 795, 872
MnlI CCTC 7 cut(s) 91, 101, 116, 154, 637, 696, 736
Mox20I TGGCCA 1 cut(s) 506
MscI TGGCCA 1 cut(s) 506
MseI TTAA 3 cut(s) 333, 732, 932
MslI CAYNNNNRTG 1 cut(s) 792
Msp20I TGGCCA 1 cut(s) 506
MspI CCGG 2 cut(s) 392, 641
MvnI CGCG 2 cut(s) 779, 832
MwoI GCNNNNNNNGC 4 cut(s) 27, 685, 754, 803
NdeII GATC 4 cut(s) 12, 205, 346, 394
NlaIII CATG 3 cut(s) 13, 550, 700
NlaIV GGNNCC 2 cut(s) 261, 396
NmeAIII GCCGAG 1 cut(s) 573
NmuCI GTSAC 1 cut(s) 387
NspI RCATGY 1 cut(s) 550
PciI ACATGT 1 cut(s) 546
PciSI GCTCTTC 1 cut(s) 579
PfeI GAWTC 3 cut(s) 24, 401, 530
PkrI GCNGC 3 cut(s) 97, 781, 831
PleI GAGTC 2 cut(s) 39, 493
PpsI GAGTC 2 cut(s) 39, 493
PscI ACATGT 1 cut(s) 546
PshBI ATTAAT 1 cut(s) 932
PsiI TTATAA 1 cut(s) 902
PspN4I GGNNCC 2 cut(s) 261, 396
PspPI GGNCC 2 cut(s) 473, 586
PstNI CAGNNNCTG 1 cut(s) 404
PsuI RGATCY 1 cut(s) 394
RsaI GTAC 3 cut(s) 261, 310, 320
RsaNI GTAC 3 cut(s) 260, 309, 319
RseI CAYNNNNRTG 1 cut(s) 792
SapI GCTCTTC 1 cut(s) 579
SaqAI TTAA 3 cut(s) 333, 732, 932
SatI GCNGC 3 cut(s) 96, 780, 830
Sau3AI GATC 4 cut(s) 12, 205, 346, 394
Sau96I GGNCC 2 cut(s) 473, 586
SchI GAGTC 2 cut(s) 39, 493
SetI ASST 8 cut(s) 32, 39, 89, 93, 108, 332, 607, 648
SfaNI GCATC 1 cut(s) 86
SinI GGWCC 1 cut(s) 473
SmiMI CAYNNNNRTG 1 cut(s) 792
Sse9I AATT 4 cut(s) 148, 265, 293, 313
SsiI CCGC 4 cut(s) 96, 779, 830, 918
SspMI CTAG 3 cut(s) 327, 378, 435
StyI CCWWGG 1 cut(s) 326
TaaI ACNGT 2 cut(s) 387, 481
TaqI TCGA 6 cut(s) 204, 468, 497, 528, 533, 629
TaqII GACCGA 1 cut(s) 490
TasI AATT 4 cut(s) 148, 265, 293, 313
TatI WGTACW 1 cut(s) 318
TauI GCSGC 3 cut(s) 98, 782, 832
TfiI GAWTC 3 cut(s) 24, 401, 530
Tru1I TTAA 3 cut(s) 333, 732, 932
Tru9I TTAA 3 cut(s) 333, 732, 932
TseFI GTSAC 1 cut(s) 387
Tsp45I GTSAC 1 cut(s) 387
TspDTI ATGAA 6 cut(s) 54, 171, 441, 603, 685, 776
VpaK11BI GGWCC 1 cut(s) 473
VspI ATTAAT 1 cut(s) 932
XapI RAATTY 1 cut(s) 293
XceI RCATGY 1 cut(s) 550
XmaJI CCTAGG 1 cut(s) 326
XspI CTAG 3 cut(s) 327, 378, 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.