pycom07g00650

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0006829
Physical Location & Seq
Reverse (-)
576932 .. 577855
924 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g00650.1

Sequence Viewer

Length: 924 bp
ATGGCGGCCATGATCACACGCAGATTCAGCTCCAAGCTTTCAAGACTCTCTCATCCTCACTCATCACTGTCTGCTTCCTCCACCTACCTCACCGCCCAAAAGCTCCAAAACCCTGAAACCCCTAACTGCTTCACTCACTCCTCCAATTCCACTTTCTCCCCAAATCCTTTCAAATCTTCATCAAACCCCACCATCACTATCGAACGCTACATAAAATCATCAAATCCAAAACCCATAAACCCTAATCTCATTTGGGTACCCAATTCAAATTCCATTACTCAAAACCCTGAATTCAAAAAAACAAATCTTGGTCTTGCTTTTACGCAATCGTGTCCAAGTTTCAGTACCAATTTGTACAACCCTAGGTTTAATGGGTTATCAGATCAAAAGCCCAGATTTTTTTCAACCTCCTCTAGTTCATCGCCATCGGATTCAGAATCTGAAAAACCCCAAAACCCTAGTGAATACCCTAGTCAAAACCCTAATTTCAAGCACCAAGAAATCGAAGGACCGACGGTGGAGCGAGACCTTTCGACATTGGCCAATGAGACCAGAGAAGTTCTGGAATCGATGGCGAAAAACATGTATAGTCTGAGCAGGACATTGGCTCTTCTGGGTTTGGCCCAACTCGGCGTCGGAGCTTGGATTTCGTACATTACCAAATCGACCCCACTGCCGGAAGTTTCAGTTCAGAGCATTCTGGCTTTCGGGTTGCCGTTTACATTGGCGTTCATGTTGAGGCAGTGTTTGAAGCCAATCTACTTCTTTAAGAAGATGGAGGAGCAAGGCAGGCTGCAGATTCTGACTCTCACTCTTCAGATTGCTAAGAATTTGAATGTGTTCTTCGTTCGGGTTCGCGGCGTTTCGTTCTTGTGCATTGCCGGTTTGTCCGTTGGAGTCTTGTTTGCTGCCCTCACAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

308

Amino Acids

34.12

Weight (kDa)

9.69

Isoelectric Point (pI)

45.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 256
AccB1I GGYRCC 1 cut(s) 256
AccII CGCG 1 cut(s) 858
AciI CCGC 3 cut(s) 5, 93, 858
AcoI YGGCCR 2 cut(s) 6, 540
AcsI RAATTY 3 cut(s) 268, 290, 829
AcuI CTGAAG 1 cut(s) 800
AcyI GRCGYC 1 cut(s) 633
AfaI GTAC 4 cut(s) 258, 346, 356, 653
AfiI CCNNNNNNNGG 1 cut(s) 676
AflIII ACRYGT 1 cut(s) 582
AgsI TTSAA 8 cut(s) 42, 172, 267, 295, 405, 490, 751, 835
AjuI GAANNNNNNNTTGG 2 cut(s) 137, 169
AluBI AGCT 4 cut(s) 30, 37, 103, 641
AluI AGCT 4 cut(s) 30, 37, 103, 641
Alw26I GTCTC 2 cut(s) 519, 542
AlwNI CAGNNNCTG 2 cut(s) 440, 802
AoxI GGCC 3 cut(s) 6, 540, 621
ApeKI GCWGC 2 cut(s) 793, 908
ApoI RAATTY 3 cut(s) 268, 290, 829
Asp700I GAANNNNTTC 1 cut(s) 839
Asp718I GGTACC 1 cut(s) 256
AspA2I CCTAGG 1 cut(s) 362
AspS9I GGNCC 2 cut(s) 509, 622
AsuHPI GGTGA 1 cut(s) 82
AvaII GGWCC 1 cut(s) 509
AvrII CCTAGG 1 cut(s) 362
BalI TGGCCA 1 cut(s) 542
BanI GGYRCC 1 cut(s) 256
BarI GAAGNNNNNNTAC 2 cut(s) 743, 775
BbvI GCAGC 2 cut(s) 780, 895
BccI CCATC 4 cut(s) 200, 433, 565, 769
BceAI ACGGC 1 cut(s) 700
BcgI CGANNNNNNTGC 2 cut(s) 655, 689
BclI TGATCA 1 cut(s) 12
BcoDI GTCTC 2 cut(s) 519, 542
BfaI CTAG 4 cut(s) 363, 414, 459, 471
BfmI CTRYAG 1 cut(s) 794
BisI GCNGC 4 cut(s) 6, 794, 859, 909
BlnI CCTAGG 1 cut(s) 362
BlsI GCNGC 4 cut(s) 7, 795, 860, 910
Bme18I GGWCC 1 cut(s) 509
BmgT120I GGNCC 2 cut(s) 509, 622
BmiI GGNNCC 1 cut(s) 258
Bsa29I ATCGAT 1 cut(s) 569
BsaHI GRCGYC 1 cut(s) 633
BsaI GGTCTC 2 cut(s) 519, 542
BsaJI CCNNGG 1 cut(s) 362
Bsc4I CCNNNNNNNGG 1 cut(s) 676
Bse118I RCCGGY 1 cut(s) 881
Bse3DI GCAATG 1 cut(s) 876
BseCI ATCGAT 1 cut(s) 569
BseDI CCNNGG 1 cut(s) 362
BseGI GGATG 1 cut(s) 52
BseLI CCNNNNNNNGG 1 cut(s) 676
BseMI GCAATG 1 cut(s) 876
BseMII CTCAG 1 cut(s) 584
BseRI GAGGAG 3 cut(s) 130, 400, 794
BseXI GCAGC 2 cut(s) 780, 895
Bsh1236I CGCG 1 cut(s) 858
BshFI GGCC 3 cut(s) 8, 542, 623
BshNI GGYRCC 1 cut(s) 256
BshVI ATCGAT 1 cut(s) 569
BsiSI CCGG 2 cut(s) 677, 882
BslI CCNNNNNNNGG 1 cut(s) 676
BsmAI GTCTC 2 cut(s) 519, 542
BsmI GAATGC 1 cut(s) 696
BsnI GGCC 3 cut(s) 8, 542, 623
Bso31I GGTCTC 2 cut(s) 519, 542
Bsp1407I TGTACA 1 cut(s) 354
Bsp143I GATC 2 cut(s) 12, 382
BspACI CCGC 3 cut(s) 5, 93, 858
BspANI GGCC 3 cut(s) 8, 542, 623
BspCNI CTCAG 1 cut(s) 585
BspDI ATCGAT 1 cut(s) 569
BspFNI CGCG 1 cut(s) 858
BspLI GGNNCC 1 cut(s) 258
BspMAI CTGCAG 1 cut(s) 798
BspQI GCTCTTC 1 cut(s) 615
BspT107I GGYRCC 1 cut(s) 256
BspTNI GGTCTC 2 cut(s) 519, 542
BsrDI GCAATG 1 cut(s) 876
BsrFI RCCGGY 1 cut(s) 881
BsrGI TGTACA 1 cut(s) 354
BssAI RCCGGY 1 cut(s) 881
BssECI CCNNGG 1 cut(s) 362
BssMI GATC 2 cut(s) 12, 382
BssNI GRCGYC 1 cut(s) 633
BssT1I CCWWGG 1 cut(s) 362
Bst4CI ACNGT 2 cut(s) 69, 517
Bst6I CTCTTC 2 cut(s) 615, 819
BstACI GRCGYC 1 cut(s) 633
BstAUI TGTACA 1 cut(s) 354
BstC8I GCNNGC 1 cut(s) 791
BstDEI CTNAG 2 cut(s) 593, 825
BstF5I GGATG 1 cut(s) 52
BstFNI CGCG 1 cut(s) 858
BstKTI GATC 2 cut(s) 15, 385
BstMAI GTCTC 2 cut(s) 519, 542
BstMBI GATC 2 cut(s) 12, 382
BstMWI GCNNNNNNNGC 2 cut(s) 27, 790
BstNSI RCATGY 1 cut(s) 586
BstSFI CTRYAG 1 cut(s) 794
BstUI CGCG 1 cut(s) 858
BstV1I GCAGC 2 cut(s) 780, 895
Bsu15I ATCGAT 1 cut(s) 569
BsuRI GGCC 3 cut(s) 8, 542, 623
BsuTUI ATCGAT 1 cut(s) 569
BtgZI GCGATG 1 cut(s) 405
BtsCI GGATG 1 cut(s) 52
BtsI GCAGTG 2 cut(s) 671, 749
BtsIMutI CAGTG 3 cut(s) 65, 671, 749
Cac8I GCNNGC 1 cut(s) 791
CaiI CAGNNNCTG 2 cut(s) 440, 802
Cfr10I RCCGGY 1 cut(s) 881
Cfr13I GGNCC 2 cut(s) 509, 622
ClaI ATCGAT 1 cut(s) 569
CseI GACGC 1 cut(s) 622
Csp6I GTAC 4 cut(s) 257, 345, 355, 652
CviAII CATG 3 cut(s) 10, 583, 733
CviQI GTAC 4 cut(s) 257, 345, 355, 652
DdeI CTNAG 2 cut(s) 593, 825
DpnI GATC 2 cut(s) 14, 384
DpnII GATC 2 cut(s) 12, 382
EaeI YGGCCR 2 cut(s) 6, 540
Eam1104I CTCTTC 2 cut(s) 615, 819
EarI CTCTTC 2 cut(s) 615, 819
Eco130I CCWWGG 1 cut(s) 362
Eco31I GGTCTC 2 cut(s) 519, 542
Eco47I GGWCC 1 cut(s) 509
Eco57I CTGAAG 1 cut(s) 800
EcoRI GAATTC 1 cut(s) 290
EcoT14I CCWWGG 1 cut(s) 362
ErhI CCWWGG 1 cut(s) 362
FaeI CATG 3 cut(s) 13, 586, 736
FaiI YATR 6 cut(s) 11, 212, 236, 584, 588, 734
FatI CATG 3 cut(s) 9, 582, 732
FbaI TGATCA 1 cut(s) 12
Fnu4HI GCNGC 4 cut(s) 6, 794, 859, 909
FokI GGATG 1 cut(s) 39
Fsp4HI GCNGC 4 cut(s) 6, 794, 859, 909
FspBI CTAG 4 cut(s) 363, 414, 459, 471
GluI GCNGC 4 cut(s) 6, 794, 859, 909
HaeIII GGCC 3 cut(s) 8, 542, 623
HapII CCGG 2 cut(s) 677, 882
HgaI GACGC 1 cut(s) 622
Hin1I GRCGYC 1 cut(s) 633
Hin1II CATG 3 cut(s) 13, 586, 736
HindIII AAGCTT 1 cut(s) 35
HinfI GANTC 8 cut(s) 24, 45, 431, 437, 566, 799, 805, 897
HpaII CCGG 2 cut(s) 677, 882
HphI GGTGA 1 cut(s) 82
Hpy166II GTNNAC 1 cut(s) 720
Hpy188I TCNGA 9 cut(s) 382, 430, 436, 442, 594, 638, 693, 804, 819
Hpy188III TCNNGA 2 cut(s) 42, 563
Hpy8I GTNNAC 1 cut(s) 720
Hpy99I CGWCG 2 cut(s) 517, 638
HpyAV CCTTC 1 cut(s) 500
HpyCH4III ACNGT 2 cut(s) 69, 517
HpyCH4V TGCA 2 cut(s) 796, 876
HpyF10VI GCNNNNNNNGC 2 cut(s) 27, 790
HpyF3I CTNAG 2 cut(s) 593, 825
Hsp92I GRCGYC 1 cut(s) 633
Hsp92II CATG 3 cut(s) 13, 586, 736
KpnI GGTACC 1 cut(s) 260
Ksp22I TGATCA 1 cut(s) 12
Kzo9I GATC 2 cut(s) 12, 382
LguI GCTCTTC 1 cut(s) 615
LmnI GCTCC 5 cut(s) 35, 108, 520, 638, 781
Lsp1109I GCAGC 2 cut(s) 780, 895
MaeI CTAG 4 cut(s) 363, 414, 459, 471
MalI GATC 2 cut(s) 14, 384
MboI GATC 2 cut(s) 12, 382
MboII GAAGA 5 cut(s) 168, 602, 784, 806, 835
MlsI TGGCCA 1 cut(s) 542
MluCI AATT 7 cut(s) 145, 262, 268, 290, 349, 484, 829
MluNI TGGCCA 1 cut(s) 542
MlyI GAGTC 3 cut(s) 39, 799, 906
MmeI TCCRAC 2 cut(s) 616, 874
MnlI CCTC 9 cut(s) 66, 88, 98, 151, 418, 421, 732, 772, 923
Mox20I TGGCCA 1 cut(s) 542
MroXI GAANNNNTTC 1 cut(s) 839
MscI TGGCCA 1 cut(s) 542
MseI TTAA 2 cut(s) 369, 768
Msp20I TGGCCA 1 cut(s) 542
MspI CCGG 2 cut(s) 677, 882
Mva1269I GAATGC 1 cut(s) 696
MvnI CGCG 1 cut(s) 858
MwoI GCNNNNNNNGC 2 cut(s) 27, 790
NdeII GATC 2 cut(s) 12, 382
NlaIII CATG 3 cut(s) 13, 586, 736
NlaIV GGNNCC 1 cut(s) 258
NmeAIII GCCGAG 1 cut(s) 609
NspI RCATGY 1 cut(s) 586
PciI ACATGT 1 cut(s) 582
PciSI GCTCTTC 1 cut(s) 615
PctI GAATGC 1 cut(s) 696
PdmI GAANNNNTTC 1 cut(s) 839
PfeI GAWTC 5 cut(s) 24, 431, 437, 566, 799
PkrI GCNGC 4 cut(s) 7, 795, 860, 910
PleI GAGTC 3 cut(s) 39, 799, 905
PpsI GAGTC 3 cut(s) 39, 799, 905
PscI ACATGT 1 cut(s) 582
PspN4I GGNNCC 1 cut(s) 258
PspPI GGNCC 2 cut(s) 509, 622
PstI CTGCAG 1 cut(s) 798
PstNI CAGNNNCTG 2 cut(s) 440, 802
RsaI GTAC 4 cut(s) 258, 346, 356, 653
RsaNI GTAC 4 cut(s) 257, 345, 355, 652
SapI GCTCTTC 1 cut(s) 615
SaqAI TTAA 2 cut(s) 369, 768
SatI GCNGC 4 cut(s) 6, 794, 859, 909
Sau3AI GATC 2 cut(s) 12, 382
Sau96I GGNCC 2 cut(s) 509, 622
SchI GAGTC 3 cut(s) 39, 799, 906
SetI ASST 9 cut(s) 32, 39, 86, 90, 105, 368, 410, 531, 643
SfcI CTRYAG 1 cut(s) 794
SinI GGWCC 1 cut(s) 509
Sse9I AATT 7 cut(s) 145, 262, 268, 290, 349, 484, 829
SsiI CCGC 3 cut(s) 5, 93, 858
SspMI CTAG 4 cut(s) 363, 414, 459, 471
StyI CCWWGG 1 cut(s) 362
TaaI ACNGT 2 cut(s) 69, 517
TaqI TCGA 5 cut(s) 201, 504, 533, 569, 665
TaqII GACCGA 1 cut(s) 526
TasI AATT 7 cut(s) 145, 262, 268, 290, 349, 484, 829
TatI WGTACW 1 cut(s) 354
TauI GCSGC 2 cut(s) 8, 861
TfiI GAWTC 5 cut(s) 24, 431, 437, 566, 799
Tru1I TTAA 2 cut(s) 369, 768
Tru9I TTAA 2 cut(s) 369, 768
TscAI CASTG 3 cut(s) 72, 678, 749
TseI GCWGC 2 cut(s) 793, 908
TspDTI ATGAA 3 cut(s) 168, 408, 721
TspGWI ACGGA 1 cut(s) 880
TspRI CASTG 3 cut(s) 72, 678, 749
VpaK11BI GGWCC 1 cut(s) 509
XapI RAATTY 3 cut(s) 268, 290, 829
XceI RCATGY 1 cut(s) 586
XcmI CCANNNNNNNNNTGG 1 cut(s) 559
XmaJI CCTAGG 1 cut(s) 362
XmnI GAANNNNTTC 1 cut(s) 839
XspI CTAG 4 cut(s) 363, 414, 459, 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.