Rorug01G0004600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0006829
Physical Location & Seq
Forward (+)
991541 .. 993737
2197 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0004600.1

Sequence Viewer

Length: 1308 bp
ATGGAGAATAGTAATAATATGATGACGGAACCAGTGATGATTTTGGATTTAAACCAACAACCTGTAGAGCAACCTCATGATTCGGTCACTGGCTTACACTCCATACTGAATGAGCTAGAAACCGCCCAAGGACGCATTGAAGAGCGTATCAGACAGCTCGAAGCAGTCACCGCTAGGCAGCGTCAGAGGTGGAGGCAAGCTCTTGTTTCCCCTCAGGTTGTGAATATCACTCCGCCTGCTGATACGCCAAATGAACAACACATTCACAGTCGGGATGTTGATGCTCTTGCCAATGCTGCGAGAGAAAGAGAGACAACAATAGAGACTGGGAAAACCAACACAACTGAGAGTACCCATTTGATAGCCAAGGCACTCGCGATGGATACAAATCCTAAGAGGGTGGAGCCTCGTATTGGGAGCTTCTTCGACTGTAATATATGCTTGGACATGGCAACAGATCCTATATTGACATGCTGCGGTCATTTGTTTTGTTGGCCATGCTTCTATCAGTTGTCTTATGCTGATTCAAATGCAAAGGAATGCCCTGTGTGCAAAGGAGAAGTGACTGATGCAACTATAATTCCAGTTTACGGGCACGGTGATGGTAACAGTTCTCAAAAGTCAAAGGAATTTGTTTCCGAGGCCCCTCCTAGGCCCCGTGCAGCCAGGATTGAGGGTGTGAGGCAGCAGCTCATTAGGCGAGGGCCATCTTCCGTAATTGAACACAGGATTCAGCTACTAGTTGGTGCATTGGGGGAGAGAAGACAGACACAGGATTTTTTTGGTTCTCATATCAATGCAATGGCTGAAAGAACTAGTTTCTTTACCAGCCGGTATCCATCACCTGCCACCGAAACATCAAGCACCCAGCACCATGATTCACTTCAAGTTTCAAGATTATTGATGCAAAGAGCTGCTTCAGTTTCTTCCCTTTCATCAGCACTGAATACTGCAGAAAGATTAGTTGAGGATCTTGAGGCATATAGTAATGATCCCCGCATGAGAAGAAACCACCAGCAATCTCCAAGTAATGATAATGGAGTTACATCTTCAAATATTGCTACAATTATACAACCAGAAAACCTGACGTCTGATACAGCTGCCGAAATCAATTCAGATGTGCTTCCAGCTGCTTCCTTTTTAAGGAGTTTTCCTGCTAGTGCGGGCTTGGAAGACCAGACCGTGAATTCAGAAATAGATTCAACATTTCGATCTTCATCTTCGAGGAGAAGAAGTAGTTTTCAGAGAGGCTCTGGTGTGAATAATAATCAGCATTCATCTGAACCCAGAAGGAGAAGGTTGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

435

Amino Acids

48.39

Weight (kDa)

6.25

Isoelectric Point (pI)

68.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_5 PF14634 143 - 185 8.1e-08 zinc-RING finger domain
zf-C3HC4_3 PF13920 144 - 190 2.9e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 144 - 182 3.2e-07 RING-type zinc-finger
zf-C3HC4_2 PF13923 144 - 184 9.4e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 144 - 184 2.2e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 853
AatII GACGTC 1 cut(s) 1091
Acc36I ACCTGC 1 cut(s) 853
AccII CGCG 1 cut(s) 377
AciI CCGC 6 cut(s) 123, 171, 233, 477, 997, 1163
AclWI GGATC 3 cut(s) 452, 978, 986
AcoI YGGCCR 1 cut(s) 494
AcsI RAATTY 2 cut(s) 629, 1186
AcuI CTGAAG 1 cut(s) 903
AcyI GRCGYC 1 cut(s) 1088
AfaI GTAC 1 cut(s) 352
AfiI CCNNNNNNNGG 4 cut(s) 413, 590, 651, 1143
AgsI TTSAA 7 cut(s) 140, 528, 722, 887, 894, 1053, 1203
AhlI ACTAGT 2 cut(s) 739, 815
AjnI CCWGG 1 cut(s) 665
AluBI AGCT 9 cut(s) 115, 157, 200, 420, 691, 736, 914, 1100, 1130
AluI AGCT 9 cut(s) 115, 157, 200, 420, 691, 736, 914, 1100, 1130
Alw26I GTCTC 2 cut(s) 305, 317
AlwI GGATC 3 cut(s) 452, 978, 986
AoxI GGCC 4 cut(s) 494, 642, 653, 704
ApeKI GCWGC 9 cut(s) 178, 296, 474, 662, 685, 688, 914, 1100, 1130
ApoI RAATTY 2 cut(s) 629, 1186
AspA2I CCTAGG 1 cut(s) 650
AspS9I GGNCC 3 cut(s) 643, 654, 704
AsuHPI GGTGA 3 cut(s) 160, 611, 834
AvrII CCTAGG 1 cut(s) 650
AxyI CCTNAGG 1 cut(s) 213
BaeGI GKGCMC 1 cut(s) 597
BalI TGGCCA 1 cut(s) 496
BbsI GAAGAC 2 cut(s) 769, 1179
BbvI GCAGC 9 cut(s) 190, 283, 461, 674, 697, 700, 901, 1087, 1117
BccI CCATC 4 cut(s) 373, 596, 715, 847
BciT130I CCWGG 1 cut(s) 667
BciVI GTATCC 2 cut(s) 376, 846
BcoDI GTCTC 2 cut(s) 305, 317
BcuI ACTAGT 2 cut(s) 739, 815
BfaI CTAG 6 cut(s) 116, 174, 651, 740, 816, 1158
BfmI CTRYAG 2 cut(s) 63, 951
BfuAI ACCTGC 1 cut(s) 853
BfuI GTATCC 2 cut(s) 376, 846
BisI GCNGC 9 cut(s) 179, 297, 475, 663, 686, 689, 915, 1101, 1131
BlnI CCTAGG 1 cut(s) 650
BlsI GCNGC 9 cut(s) 180, 298, 476, 664, 687, 690, 916, 1102, 1132
Bme1390I CCNGG 1 cut(s) 667
BmgT120I GGNCC 3 cut(s) 643, 654, 704
BmiI GGNNCC 4 cut(s) 30, 405, 645, 656
BmrFI CCNGG 1 cut(s) 667
BmrI ACTGGG 1 cut(s) 336
BmsI GCATC 3 cut(s) 271, 559, 894
BmuI ACTGGG 1 cut(s) 336
BpiI GAAGAC 2 cut(s) 769, 1179
BplI GAGNNNNNCTC 2 cut(s) 184, 216
BpuEI CTTGAG 1 cut(s) 995
BsaBI GATNNNNATC 2 cut(s) 387, 1216
BsaHI GRCGYC 1 cut(s) 1088
BsaJI CCNNGG 4 cut(s) 127, 366, 639, 650
BsaXI ACNNNNNCTCC 2 cut(s) 1219, 1249
Bsc4I CCNNNNNNNGG 4 cut(s) 413, 590, 651, 1143
Bse118I RCCGGY 1 cut(s) 831
Bse1I ACTGG 4 cut(s) 32, 94, 331, 584
Bse21I CCTNAGG 1 cut(s) 213
Bse3DI GCAATG 1 cut(s) 807
Bse8I GATNNNNATC 2 cut(s) 387, 1216
BseBI CCWGG 1 cut(s) 667
BseDI CCNNGG 4 cut(s) 127, 366, 639, 650
BseGI GGATG 1 cut(s) 280
BseJI GATNNNNATC 2 cut(s) 387, 1216
BseLI CCNNNNNNNGG 4 cut(s) 413, 590, 651, 1143
BseMI GCAATG 1 cut(s) 807
BseMII CTCAG 2 cut(s) 227, 336
BseNI ACTGG 4 cut(s) 32, 94, 331, 584
BseRI GAGGAG 1 cut(s) 1240
BseSI GKGCMC 1 cut(s) 597
BseXI GCAGC 9 cut(s) 190, 283, 461, 674, 697, 700, 901, 1087, 1117
BseYI CCCAGC 1 cut(s) 867
BsgI GTGCAG 1 cut(s) 681
Bsh1236I CGCG 1 cut(s) 377
BshFI GGCC 4 cut(s) 496, 644, 655, 706
BsiSI CCGG 1 cut(s) 832
BslI CCNNNNNNNGG 4 cut(s) 413, 590, 651, 1143
BsmAI GTCTC 2 cut(s) 305, 317
BsmI GAATGC 2 cut(s) 545, 1273
BsnI GGCC 4 cut(s) 496, 644, 655, 706
Bsp1286I GDGCHC 1 cut(s) 597
Bsp143I GATC 4 cut(s) 457, 970, 991, 1211
Bsp68I TCGCGA 1 cut(s) 377
BspACI CCGC 6 cut(s) 123, 171, 233, 477, 997, 1163
BspANI GGCC 4 cut(s) 496, 644, 655, 706
BspCNI CTCAG 2 cut(s) 226, 337
BspFNI CGCG 1 cut(s) 377
BspHI TCATGA 1 cut(s) 76
BspLI GGNNCC 4 cut(s) 30, 405, 645, 656
BspMAI CTGCAG 1 cut(s) 955
BspMI ACCTGC 1 cut(s) 853
BspPI GGATC 3 cut(s) 452, 978, 986
BspQI GCTCTTC 1 cut(s) 135
BsrDI GCAATG 1 cut(s) 807
BsrFI RCCGGY 1 cut(s) 831
BsrI ACTGG 4 cut(s) 32, 94, 331, 584
BssAI RCCGGY 1 cut(s) 831
BssECI CCNNGG 4 cut(s) 127, 366, 639, 650
BssMI GATC 4 cut(s) 457, 970, 991, 1211
BssNI GRCGYC 1 cut(s) 1088
BssT1I CCWWGG 3 cut(s) 127, 366, 650
Bst2UI CCWGG 1 cut(s) 667
Bst4CI ACNGT 5 cut(s) 269, 431, 599, 611, 1183
Bst6I CTCTTC 1 cut(s) 135
BstACI GRCGYC 1 cut(s) 1088
BstC8I GCNNGC 3 cut(s) 198, 237, 1165
BstDEI CTNAG 3 cut(s) 213, 345, 393
BstENI CCTNNNNNAGG 1 cut(s) 1141
BstF5I GGATG 1 cut(s) 280
BstFNI CGCG 1 cut(s) 377
BstKTI GATC 4 cut(s) 460, 973, 994, 1214
BstMAI GTCTC 2 cut(s) 305, 317
BstMBI GATC 4 cut(s) 457, 970, 991, 1211
BstMWI GCNNNNNNNGC 4 cut(s) 170, 296, 549, 697
BstNI CCWGG 1 cut(s) 667
BstNSI RCATGY 1 cut(s) 474
BstSCI CCNGG 1 cut(s) 665
BstSFI CTRYAG 2 cut(s) 63, 951
BstSLI GKGCMC 1 cut(s) 597
BstUI CGCG 1 cut(s) 377
BstV1I GCAGC 9 cut(s) 190, 283, 461, 674, 697, 700, 901, 1087, 1117
BstV2I GAAGAC 2 cut(s) 769, 1179
BstX2I RGATCY 2 cut(s) 457, 970
BstYI RGATCY 2 cut(s) 457, 970
Bsu36I CCTNAGG 1 cut(s) 213
BsuI GTATCC 2 cut(s) 376, 846
BsuRI GGCC 4 cut(s) 496, 644, 655, 706
BtgZI GCGATG 1 cut(s) 392
BtsCI GGATG 1 cut(s) 280
BtsIMutI CAGTG 3 cut(s) 39, 87, 941
BtuMI TCGCGA 1 cut(s) 377
BveI ACCTGC 1 cut(s) 853
Cac8I GCNNGC 3 cut(s) 198, 237, 1165
CciI TCATGA 1 cut(s) 76
Cfr10I RCCGGY 1 cut(s) 831
Cfr13I GGNCC 3 cut(s) 643, 654, 704
CseI GACGC 2 cut(s) 141, 170
Csp6I GTAC 1 cut(s) 351
CviAII CATG 6 cut(s) 77, 448, 471, 498, 875, 1000
CviQI GTAC 1 cut(s) 351
DdeI CTNAG 3 cut(s) 213, 345, 393
DpnI GATC 4 cut(s) 459, 972, 993, 1213
DpnII GATC 4 cut(s) 457, 970, 991, 1211
DraI TTTAAA 1 cut(s) 51
EaeI YGGCCR 1 cut(s) 494
Eam1104I CTCTTC 1 cut(s) 135
EarI CTCTTC 1 cut(s) 135
EciI GGCGGA 1 cut(s) 222
Eco130I CCWWGG 3 cut(s) 127, 366, 650
Eco57I CTGAAG 1 cut(s) 903
Eco81I CCTNAGG 1 cut(s) 213
EcoNI CCTNNNNNAGG 1 cut(s) 1141
EcoO109I RGGNCCY 2 cut(s) 643, 654
EcoRI GAATTC 1 cut(s) 1186
EcoRII CCWGG 1 cut(s) 665
EcoT14I CCWWGG 3 cut(s) 127, 366, 650
ErhI CCWWGG 3 cut(s) 127, 366, 650
FaeI CATG 6 cut(s) 80, 451, 474, 501, 878, 1003
FalI AAGNNNNNCTT 2 cut(s) 901, 933
FatI CATG 6 cut(s) 76, 447, 470, 497, 874, 999
FauI CCCGC 2 cut(s) 1004, 1156
Fnu4HI GCNGC 9 cut(s) 179, 297, 475, 663, 686, 689, 915, 1101, 1131
FokI GGATG 1 cut(s) 287
Fsp4HI GCNGC 9 cut(s) 179, 297, 475, 663, 686, 689, 915, 1101, 1131
FspBI CTAG 6 cut(s) 116, 174, 651, 740, 816, 1158
GluI GCNGC 9 cut(s) 179, 297, 475, 663, 686, 689, 915, 1101, 1131
GsaI CCCAGC 1 cut(s) 871
HaeIII GGCC 4 cut(s) 496, 644, 655, 706
HapII CCGG 1 cut(s) 832
HgaI GACGC 2 cut(s) 141, 170
Hin1I GRCGYC 1 cut(s) 1088
Hin1II CATG 6 cut(s) 80, 451, 474, 501, 878, 1003
HinfI GANTC 5 cut(s) 80, 524, 730, 878, 1199
HpaII CCGG 1 cut(s) 832
HphI GGTGA 3 cut(s) 160, 611, 834
Hpy166II GTNNAC 1 cut(s) 589
Hpy188I TCNGA 8 cut(s) 152, 186, 640, 1093, 1117, 1192, 1245, 1282
Hpy188III TCNNGA 5 cut(s) 77, 272, 376, 894, 974
Hpy8I GTNNAC 1 cut(s) 589
HpyAV CCTTC 2 cut(s) 1284, 1290
HpyCH4III ACNGT 5 cut(s) 269, 431, 599, 611, 1183
HpyCH4IV ACGT 1 cut(s) 1088
HpyCH4V TGCA 8 cut(s) 533, 552, 572, 662, 749, 800, 907, 953
HpyF10VI GCNNNNNNNGC 4 cut(s) 170, 296, 549, 697
HpyF3I CTNAG 3 cut(s) 213, 345, 393
HpySE526I ACGT 1 cut(s) 1088
Hsp92I GRCGYC 1 cut(s) 1088
Hsp92II CATG 6 cut(s) 80, 451, 474, 501, 878, 1003
Kzo9I GATC 4 cut(s) 457, 970, 991, 1211
LguI GCTCTTC 1 cut(s) 135
LmnI GCTCC 2 cut(s) 403, 417
Lsp1109I GCAGC 9 cut(s) 190, 283, 461, 674, 697, 700, 901, 1087, 1117
LweI GCATC 3 cut(s) 271, 559, 894
MaeI CTAG 6 cut(s) 116, 174, 651, 740, 816, 1158
MaeII ACGT 1 cut(s) 1088
MaeIII GTNAC 5 cut(s) 85, 166, 562, 605, 1042
MalI GATC 4 cut(s) 459, 972, 993, 1213
MboI GATC 4 cut(s) 457, 970, 991, 1211
MflI RGATCY 2 cut(s) 457, 970
MhlI GDGCHC 1 cut(s) 597
MlsI TGGCCA 1 cut(s) 496
MluCI AATT 6 cut(s) 579, 629, 717, 1065, 1111, 1186
MluNI TGGCCA 1 cut(s) 496
Mox20I TGGCCA 1 cut(s) 496
MscI TGGCCA 1 cut(s) 496
MseI TTAA 2 cut(s) 50, 1142
MslI CAYNNNNRTG 2 cut(s) 600, 795
Msp20I TGGCCA 1 cut(s) 496
MspA1I CMGCKG 2 cut(s) 1100, 1130
MspI CCGG 1 cut(s) 832
MspR9I CCNGG 1 cut(s) 667
Mva1269I GAATGC 2 cut(s) 545, 1273
MvaI CCWGG 1 cut(s) 667
MvnI CGCG 1 cut(s) 377
MwoI GCNNNNNNNGC 4 cut(s) 170, 296, 549, 697
NdeII GATC 4 cut(s) 457, 970, 991, 1211
NlaIII CATG 6 cut(s) 80, 451, 474, 501, 878, 1003
NlaIV GGNNCC 4 cut(s) 30, 405, 645, 656
NmuCI GTSAC 3 cut(s) 85, 166, 562
NruI TCGCGA 1 cut(s) 377
NspI RCATGY 1 cut(s) 474
PagI TCATGA 1 cut(s) 76
PaqCI CACCTGC 1 cut(s) 853
PciSI GCTCTTC 1 cut(s) 135
PctI GAATGC 2 cut(s) 545, 1273
PfeI GAWTC 5 cut(s) 80, 524, 730, 878, 1199
PkrI GCNGC 9 cut(s) 180, 298, 476, 664, 687, 690, 916, 1102, 1132
Psp6I CCWGG 1 cut(s) 665
PspFI CCCAGC 1 cut(s) 867
PspGI CCWGG 1 cut(s) 665
PspN4I GGNNCC 4 cut(s) 30, 405, 645, 656
PspPI GGNCC 3 cut(s) 643, 654, 704
PstI CTGCAG 1 cut(s) 955
PsuI RGATCY 2 cut(s) 457, 970
PvuII CAGCTG 2 cut(s) 1100, 1130
RruI TCGCGA 1 cut(s) 377
RsaI GTAC 1 cut(s) 352
RsaNI GTAC 1 cut(s) 351
RseI CAYNNNNRTG 2 cut(s) 600, 795
SapI GCTCTTC 1 cut(s) 135
SaqAI TTAA 2 cut(s) 50, 1142
SatI GCNGC 9 cut(s) 179, 297, 475, 663, 686, 689, 915, 1101, 1131
Sau3AI GATC 4 cut(s) 457, 970, 991, 1211
Sau96I GGNCC 3 cut(s) 643, 654, 704
ScrFI CCNGG 1 cut(s) 667
SduI GDGCHC 1 cut(s) 597
SfaNI GCATC 3 cut(s) 271, 559, 894
SfcI CTRYAG 2 cut(s) 63, 951
SmiMI CAYNNNNRTG 2 cut(s) 600, 795
SmlI CTYRAG 1 cut(s) 974
SmoI CTYRAG 1 cut(s) 974
SpeI ACTAGT 2 cut(s) 739, 815
Sse9I AATT 6 cut(s) 579, 629, 717, 1065, 1111, 1186
SsiI CCGC 6 cut(s) 123, 171, 233, 477, 997, 1163
SspI AATATT 1 cut(s) 1057
SspMI CTAG 6 cut(s) 116, 174, 651, 740, 816, 1158
StyD4I CCNGG 1 cut(s) 665
StyI CCWWGG 3 cut(s) 127, 366, 650
TaaI ACNGT 5 cut(s) 269, 431, 599, 611, 1183
TaiI ACGT 1 cut(s) 1091
TaqI TCGA 4 cut(s) 159, 426, 1210, 1223
TaqII GACCGA 1 cut(s) 73
TasI AATT 6 cut(s) 579, 629, 717, 1065, 1111, 1186
TfiI GAWTC 5 cut(s) 80, 524, 730, 878, 1199
Tru1I TTAA 2 cut(s) 50, 1142
Tru9I TTAA 2 cut(s) 50, 1142
TscAI CASTG 3 cut(s) 39, 94, 948
TseFI GTSAC 3 cut(s) 85, 166, 562
TseI GCWGC 9 cut(s) 178, 296, 474, 662, 685, 688, 914, 1100, 1130
Tsp45I GTSAC 3 cut(s) 85, 166, 562
TspDTI ATGAA 4 cut(s) 267, 924, 1206, 1266
TspGWI ACGGA 2 cut(s) 41, 703
TspRI CASTG 3 cut(s) 39, 94, 948
XagI CCTNNNNNAGG 1 cut(s) 1141
XapI RAATTY 2 cut(s) 629, 1186
XceI RCATGY 1 cut(s) 474
XmaJI CCTAGG 1 cut(s) 650
XspI CTAG 6 cut(s) 116, 174, 651, 740, 816, 1158
ZraI GACGTC 1 cut(s) 1089
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.