pycom03g01610

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0007058
Physical Location & Seq
Reverse (-)
1198121 .. 1200058
1938 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 888 bp
ATGCATGGTGACATGGAAATCCAGATTCACCGCATTGAGCAAGAAGCATACTGTTCAGTTCTAAGGGCGTTTAGTGCTCAATCTGATGCCCTCACCTGGGATAAGGAAACTTTAATTACGGAACTTAGAAAGGAGCTGAGAGTATCTGATGAGGAACACAGAGAACTTTTGTCCAGGGTCAATGCCGATGACATGATCCAGAGATTAAGGGAATGGAGAAAAACAAGTGGGGTTCAACATGGTACACTTAATGCTGCTCAGCCTGTTCATGACCCCCTACCTAGTCCAACTGTTTCAGCATCACGCAAGAAACAGAAAACATCACAGTCCTTATCCCTCGGTGCTCCAACTCCTGCATTGCCTCCATCCATGCAACAATCCTCTGCAGCTTTGAGACGAGGTCCTCCTCCAGGACCTCGGAACAAGAAACCAAAATCTTCCTCACAGTACCCTTCCACAAATATTCCTGGAAGGCCCCAAGCCACTAATCGAGGTCCTGCCGGTGCCTTTGTGGAAAATGAGCCTGCTGAGGGTGAAACTGATGATCCGTTAGTAGGAAGAAAAGTTTGGACAAGGTGGCCTGAAGACAATCACTTCTATGAGGCTGTTATAACTAATTTCAACCGTGCTGAGGGGCTACATGCTCTGGTTTATGATATTGGTACAGTGGATGAGACATGGGAATGGGTCAATCTAAAAGAGAGATATCTCCTGAAGATATTCGGTGGGAAGCTGATGGTCCTGGGATTTCTCGTAAAGGCAGTCGACCCGGACCTGGACCTGGCCGTGGGAATAAAAAGTCCACGGCACGTGGCGGTGCAGTTTCAGGTGCAGGAAGAGGTAGAGGGACTGCAAAGGGTTCCAAGAAGGATTTGCCTCCGCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT LBR_tudor

Protein Analysis

296

Amino Acids

33.18

Weight (kDa)

7.82

Isoelectric Point (pI)

47.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENT PF03735 8 - 75 1.2e-30 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 611
AccB1I GGYRCC 1 cut(s) 503
AccI GTMKAC 1 cut(s) 765
AciI CCGC 3 cut(s) 31, 815, 880
AclWI GGATC 2 cut(s) 190, 539
AcoI YGGCCR 1 cut(s) 783
AcuI CTGAAG 2 cut(s) 603, 734
AcvI CACGTG 1 cut(s) 811
AfaI GTAC 3 cut(s) 244, 449, 664
AfiI CCNNNNNNNGG 9 cut(s) 96, 97, 410, 530, 554, 631, 775, 781, 787
AgsI TTSAA 2 cut(s) 236, 622
AjnI CCWGG 7 cut(s) 95, 173, 409, 466, 741, 774, 780
AjuI GAANNNNNNNTTGG 2 cut(s) 550, 582
AluBI AGCT 3 cut(s) 136, 389, 733
AluI AGCT 3 cut(s) 136, 389, 733
Alw21I GWGCWC 2 cut(s) 79, 346
Alw26I GTCTC 2 cut(s) 388, 668
AlwI GGATC 2 cut(s) 190, 539
AoxI GGCC 3 cut(s) 473, 578, 783
ApeKI GCWGC 2 cut(s) 254, 386
Asp700I GAANNNNTTC 1 cut(s) 719
AspS9I GGNCC 7 cut(s) 401, 413, 474, 494, 739, 772, 778
AsuC2I CCSGG 1 cut(s) 770
AsuHPI GGTGA 4 cut(s) 20, 20, 85, 545
AvaII GGWCC 6 cut(s) 401, 413, 494, 739, 772, 778
BanI GGYRCC 1 cut(s) 503
BbrPI CACGTG 1 cut(s) 811
BbsI GAAGAC 1 cut(s) 591
Bbv12I GWGCWC 2 cut(s) 79, 346
BbvCI CCTCAGC 2 cut(s) 528, 630
BbvI GCAGC 2 cut(s) 241, 398
BccI CCATC 2 cut(s) 373, 730
BceAI ACGGC 2 cut(s) 770, 821
BciT130I CCWGG 7 cut(s) 97, 175, 411, 468, 743, 776, 782
BcnI CCSGG 1 cut(s) 770
BcoDI GTCTC 2 cut(s) 388, 668
BfaI CTAG 1 cut(s) 282
BfmI CTRYAG 1 cut(s) 384
BisI GCNGC 2 cut(s) 255, 387
BlpI GCTNAGC 1 cut(s) 258
BlsI GCNGC 2 cut(s) 256, 388
Bme1390I CCNGG 8 cut(s) 97, 175, 411, 468, 743, 770, 776, 782
Bme18I GGWCC 6 cut(s) 401, 413, 494, 739, 772, 778
BmgT120I GGNCC 7 cut(s) 401, 413, 474, 494, 739, 772, 778
BmiI GGNNCC 3 cut(s) 476, 505, 861
BmrFI CCNGG 8 cut(s) 97, 175, 411, 468, 743, 770, 776, 782
BmsI GCATC 2 cut(s) 76, 308
BpiI GAAGAC 1 cut(s) 591
BpmI CTGGAG 1 cut(s) 393
Bpu10I CCTNAGC 2 cut(s) 528, 630
Bpu1102I GCTNAGC 1 cut(s) 258
BpuMI CCSGG 1 cut(s) 770
BsaAI YACGTR 1 cut(s) 811
BsaJI CCNNGG 7 cut(s) 96, 174, 337, 416, 742, 786, 803
Bsc4I CCNNNNNNNGG 9 cut(s) 96, 97, 410, 530, 554, 631, 775, 781, 787
Bse118I RCCGGY 1 cut(s) 500
Bse3DI GCAATG 1 cut(s) 356
BseBI CCWGG 7 cut(s) 97, 175, 411, 468, 743, 776, 782
BseDI CCNNGG 7 cut(s) 96, 174, 337, 416, 742, 786, 803
BseGI GGATG 2 cut(s) 365, 676
BseLI CCNNNNNNNGG 9 cut(s) 96, 97, 410, 530, 554, 631, 775, 781, 787
BseMI GCAATG 1 cut(s) 356
BseMII CTCAG 4 cut(s) 128, 272, 519, 621
BseRI GAGGAG 1 cut(s) 396
BseXI GCAGC 2 cut(s) 241, 398
BsgI GTGCAG 2 cut(s) 839, 851
BshFI GGCC 3 cut(s) 475, 580, 785
BshNI GGYRCC 1 cut(s) 503
BsiHKAI GWGCWC 2 cut(s) 79, 346
BsiSI CCGG 2 cut(s) 501, 770
BslFI GGGAC 1 cut(s) 861
BslI CCNNNNNNNGG 9 cut(s) 96, 97, 410, 530, 554, 631, 775, 781, 787
BsmAI GTCTC 2 cut(s) 388, 668
BsmBI CGTCTC 1 cut(s) 388
BsmFI GGGAC 1 cut(s) 861
BsnI GGCC 3 cut(s) 475, 580, 785
Bsp1286I GDGCHC 2 cut(s) 79, 346
Bsp143I GATC 2 cut(s) 195, 544
Bsp1720I GCTNAGC 1 cut(s) 258
BspACI CCGC 3 cut(s) 31, 815, 880
BspANI GGCC 3 cut(s) 475, 580, 785
BspCNI CTCAG 4 cut(s) 129, 271, 520, 622
BspHI TCATGA 1 cut(s) 268
BspLI GGNNCC 3 cut(s) 476, 505, 861
BspMAI CTGCAG 1 cut(s) 388
BspPI GGATC 2 cut(s) 190, 539
BspT107I GGYRCC 1 cut(s) 503
BsrDI GCAATG 1 cut(s) 356
BsrFI RCCGGY 1 cut(s) 500
BssAI RCCGGY 1 cut(s) 500
BssECI CCNNGG 7 cut(s) 96, 174, 337, 416, 742, 786, 803
BssMI GATC 2 cut(s) 195, 544
Bst2UI CCWGG 7 cut(s) 97, 175, 411, 468, 743, 776, 782
Bst4CI ACNGT 6 cut(s) 53, 292, 327, 447, 626, 667
Bst6I CTCTTC 1 cut(s) 831
BstBAI YACGTR 1 cut(s) 811
BstC8I GCNNGC 1 cut(s) 525
BstDEI CTNAG 6 cut(s) 62, 125, 137, 258, 528, 630
BstDSI CCRYGG 2 cut(s) 786, 803
BstENI CCTNNNNNAGG 1 cut(s) 408
BstF5I GGATG 2 cut(s) 365, 676
BstKTI GATC 2 cut(s) 198, 547
BstMAI GTCTC 2 cut(s) 388, 668
BstMBI GATC 2 cut(s) 195, 544
BstMWI GCNNNNNNNGC 1 cut(s) 74
BstNI CCWGG 7 cut(s) 97, 175, 411, 468, 743, 776, 782
BstNSI RCATGY 1 cut(s) 644
BstSCI CCNGG 8 cut(s) 95, 173, 409, 466, 741, 768, 774, 780
BstSFI CTRYAG 1 cut(s) 384
BstV1I GCAGC 2 cut(s) 241, 398
BstV2I GAAGAC 1 cut(s) 591
BsuRI GGCC 3 cut(s) 475, 580, 785
BtgI CCRYGG 2 cut(s) 786, 803
BtsCI GGATG 2 cut(s) 365, 676
BtsIMutI CAGTG 1 cut(s) 672
Cac8I GCNNGC 1 cut(s) 525
CciI TCATGA 1 cut(s) 268
Cfr10I RCCGGY 1 cut(s) 500
Cfr13I GGNCC 7 cut(s) 401, 413, 474, 494, 739, 772, 778
Csp6I GTAC 3 cut(s) 243, 448, 663
CviAII CATG 8 cut(s) 5, 13, 193, 239, 269, 370, 641, 678
CviQI GTAC 3 cut(s) 243, 448, 663
DdeI CTNAG 6 cut(s) 62, 125, 137, 258, 528, 630
DpnI GATC 2 cut(s) 197, 546
DpnII GATC 2 cut(s) 195, 544
EaeI YGGCCR 1 cut(s) 783
Eam1104I CTCTTC 1 cut(s) 831
EarI CTCTTC 1 cut(s) 831
Eco32I GATATC 1 cut(s) 707
Eco47I GGWCC 6 cut(s) 401, 413, 494, 739, 772, 778
Eco57I CTGAAG 2 cut(s) 603, 734
Eco72I CACGTG 1 cut(s) 811
EcoNI CCTNNNNNAGG 1 cut(s) 408
EcoO109I RGGNCCY 4 cut(s) 401, 413, 474, 494
EcoRII CCWGG 7 cut(s) 95, 173, 409, 466, 741, 774, 780
EcoRV GATATC 1 cut(s) 707
EcoT22I ATGCAT 1 cut(s) 6
Esp3I CGTCTC 1 cut(s) 388
FaeI CATG 8 cut(s) 8, 16, 196, 242, 272, 373, 644, 681
FaqI GGGAC 1 cut(s) 861
FatI CATG 8 cut(s) 4, 12, 192, 238, 268, 369, 640, 677
FblI GTMKAC 1 cut(s) 765
Fnu4HI GCNGC 2 cut(s) 255, 387
FokI GGATG 2 cut(s) 352, 683
Fsp4HI GCNGC 2 cut(s) 255, 387
FspBI CTAG 1 cut(s) 282
GluI GCNGC 2 cut(s) 255, 387
GsuI CTGGAG 1 cut(s) 393
HaeIII GGCC 3 cut(s) 475, 580, 785
HapII CCGG 2 cut(s) 501, 770
Hin1II CATG 8 cut(s) 8, 16, 196, 242, 272, 373, 644, 681
HincII GTYRAC 1 cut(s) 766
HindII GTYRAC 1 cut(s) 766
HinfI GANTC 1 cut(s) 25
HpaII CCGG 2 cut(s) 501, 770
HphI GGTGA 4 cut(s) 20, 20, 85, 545
Hpy166II GTNNAC 3 cut(s) 245, 766, 803
Hpy188I TCNGA 3 cut(s) 85, 148, 420
Hpy188III TCNNGA 4 cut(s) 22, 199, 269, 712
Hpy8I GTNNAC 3 cut(s) 245, 766, 803
HpyAV CCTTC 3 cut(s) 462, 465, 861
HpyCH4III ACNGT 6 cut(s) 53, 292, 327, 447, 626, 667
HpyCH4IV ACGT 1 cut(s) 810
HpyCH4V TGCA 7 cut(s) 4, 356, 373, 386, 820, 832, 853
HpyF10VI GCNNNNNNNGC 1 cut(s) 74
HpyF3I CTNAG 6 cut(s) 62, 125, 137, 258, 528, 630
HpySE526I ACGT 1 cut(s) 810
Hsp92II CATG 8 cut(s) 8, 16, 196, 242, 272, 373, 644, 681
Kzo9I GATC 2 cut(s) 195, 544
LmnI GCTCC 2 cut(s) 133, 349
Lsp1109I GCAGC 2 cut(s) 241, 398
LweI GCATC 2 cut(s) 76, 308
MaeI CTAG 1 cut(s) 282
MaeII ACGT 1 cut(s) 810
MaeIII GTNAC 1 cut(s) 8
MalI GATC 2 cut(s) 197, 546
MboI GATC 2 cut(s) 195, 544
MboII GAAGA 5 cut(s) 429, 570, 596, 727, 848
MhlI GDGCHC 2 cut(s) 79, 346
MluCI AATT 2 cut(s) 114, 616
MmeI TCCRAC 2 cut(s) 311, 371
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 719
MseI TTAA 3 cut(s) 113, 206, 249
MslI CAYNNNNRTG 2 cut(s) 597, 682
MspI CCGG 2 cut(s) 501, 770
MspR9I CCNGG 8 cut(s) 97, 175, 411, 468, 743, 770, 776, 782
MvaI CCWGG 7 cut(s) 97, 175, 411, 468, 743, 776, 782
MwoI GCNNNNNNNGC 1 cut(s) 74
NciI CCSGG 1 cut(s) 770
NdeII GATC 2 cut(s) 195, 544
NlaIII CATG 8 cut(s) 8, 16, 196, 242, 272, 373, 644, 681
NlaIV GGNNCC 3 cut(s) 476, 505, 861
NmuCI GTSAC 1 cut(s) 8
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 644
PagI TCATGA 1 cut(s) 268
PdmI GAANNNNTTC 1 cut(s) 719
PfeI GAWTC 1 cut(s) 25
PflFI GACNNNGTC 1 cut(s) 399
PfoI TCCNGGA 2 cut(s) 409, 466
PkrI GCNGC 2 cut(s) 256, 388
PmaCI CACGTG 1 cut(s) 811
PmlI CACGTG 1 cut(s) 811
Ppu21I YACGTR 1 cut(s) 811
PpuMI RGGWCCY 3 cut(s) 401, 413, 494
PsiI TTATAA 1 cut(s) 611
Psp5II RGGWCCY 3 cut(s) 401, 413, 494
Psp6I CCWGG 7 cut(s) 95, 173, 409, 466, 741, 774, 780
PspCI CACGTG 1 cut(s) 811
PspGI CCWGG 7 cut(s) 95, 173, 409, 466, 741, 774, 780
PspN4I GGNNCC 3 cut(s) 476, 505, 861
PspPI GGNCC 7 cut(s) 401, 413, 474, 494, 739, 772, 778
PspPPI RGGWCCY 3 cut(s) 401, 413, 494
PstI CTGCAG 1 cut(s) 388
PsyI GACNNNGTC 1 cut(s) 399
RsaI GTAC 3 cut(s) 244, 449, 664
RsaNI GTAC 3 cut(s) 243, 448, 663
RseI CAYNNNNRTG 2 cut(s) 597, 682
SalI GTCGAC 1 cut(s) 764
SaqAI TTAA 3 cut(s) 113, 206, 249
SatI GCNGC 2 cut(s) 255, 387
Sau3AI GATC 2 cut(s) 195, 544
Sau96I GGNCC 7 cut(s) 401, 413, 474, 494, 739, 772, 778
ScrFI CCNGG 8 cut(s) 97, 175, 411, 468, 743, 770, 776, 782
SduI GDGCHC 2 cut(s) 79, 346
SfaNI GCATC 2 cut(s) 76, 308
SfcI CTRYAG 1 cut(s) 384
SinI GGWCC 6 cut(s) 401, 413, 494, 739, 772, 778
SmiMI CAYNNNNRTG 2 cut(s) 597, 682
Sse9I AATT 2 cut(s) 114, 616
SsiI CCGC 3 cut(s) 31, 815, 880
SspI AATATT 1 cut(s) 463
SspMI CTAG 1 cut(s) 282
StyD4I CCNGG 8 cut(s) 95, 173, 409, 466, 741, 768, 774, 780
TaaI ACNGT 6 cut(s) 53, 292, 327, 447, 626, 667
TaiI ACGT 1 cut(s) 813
TaqI TCGA 2 cut(s) 490, 765
TasI AATT 2 cut(s) 114, 616
TfiI GAWTC 1 cut(s) 25
Tru1I TTAA 3 cut(s) 113, 206, 249
Tru9I TTAA 3 cut(s) 113, 206, 249
TscAI CASTG 1 cut(s) 672
TseFI GTSAC 1 cut(s) 8
TseI GCWGC 2 cut(s) 254, 386
Tsp45I GTSAC 1 cut(s) 8
TspDTI ATGAA 1 cut(s) 257
TspGWI ACGGA 2 cut(s) 134, 537
TspRI CASTG 1 cut(s) 672
Tth111I GACNNNGTC 1 cut(s) 399
VpaK11BI GGWCC 6 cut(s) 401, 413, 494, 739, 772, 778
XagI CCTNNNNNAGG 1 cut(s) 408
XceI RCATGY 1 cut(s) 644
XmiI GTMKAC 1 cut(s) 765
XmnI GAANNNNTTC 1 cut(s) 719
XspI CTAG 1 cut(s) 282
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.