pycom03g06640

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0016808
Physical Location & Seq
Reverse (-)
5203708 .. 5204694
987 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 750 bp
ATGGATCCGTGCAATGTTGTTAAGGATTTAAATTTTTATGGACTAGCAAACGGTACTGCAAGAGGAGGCGTTCCTGCTGCAAAAATTGCAGCATATCAAGTCTGTATTCCACGCTGCCAAGATCACACCTTACTGGCTGCTTTTGATGATGCTATTGCTGATGGAGTTAAGATCATTACAATTTCACTTGGACAATTAGATCCGATTGGTTTGGATGAGGATGCCATTGCAATTGGTGCTTTTCATGCAATGAAGAAAGGGATAATGACTTCACAGGCTGCAGGCAATTTGGGTCTTGGTATTGCTTCTGTGTCAAGTGTAGCACCATGGATATTGACAGTTGCGGCAAGTAACATGGATCGCTACTTCGTTGACAAGATTGTTCTTGCAAGTGGAACCACCCTTGTTGGTAATTCAATTAACACTTTCATATTAAATGGAACAAGTTTTCCTCTGATATATGGAAAAGATGCTGGAACGAAATATTGCACTGCAGAAGATGCACGAAATTGTGAAGAAGGATGCTTGGATAGTGATTTGGTTAAGGGAAAGATAGTGGTATGCGATGAGATACAAGGAGATAGAGCGGCATACAAATCTGGAGCACTTGGTTCAGTTACACTTAATGAAGTTGGATATAATGTTTCTCTTGTTCCCTTATTGGCCTCAACAACGTTAATGAAGGAAGAGTATAATTTGGTCAGGTCCTACATGAACTCCACCAGGTATCGATTCTTTCGATACTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

250

Amino Acids

26.52

Weight (kDa)

5.21

Isoelectric Point (pI)

15.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 20 - 119 3.8e-08 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 587
AciI CCGC 2 cut(s) 344, 587
AclI AACGTT 1 cut(s) 674
AclWI GGATC 3 cut(s) 12, 194, 366
AcsI RAATTY 1 cut(s) 31
AfaI GTAC 1 cut(s) 55
AgsI TTSAA 1 cut(s) 417
AjnI CCWGG 1 cut(s) 722
AloI GAACNNNNNNTCC 2 cut(s) 433, 465
Alw21I GWGCWC 1 cut(s) 607
AlwI GGATC 3 cut(s) 12, 194, 366
AoxI GGCC 1 cut(s) 663
ApeKI GCWGC 5 cut(s) 77, 89, 114, 137, 278
ApoI RAATTY 1 cut(s) 31
AspS9I GGNCC 1 cut(s) 705
AvaII GGWCC 1 cut(s) 705
BamHI GGATCC 1 cut(s) 4
Bbv12I GWGCWC 1 cut(s) 607
BbvI GCAGC 5 cut(s) 64, 101, 101, 124, 265
BccI CCATC 1 cut(s) 155
BciT130I CCWGG 1 cut(s) 724
BfaI CTAG 1 cut(s) 44
BfmI CTRYAG 2 cut(s) 279, 492
BisI GCNGC 7 cut(s) 78, 90, 115, 138, 279, 345, 588
BlsI GCNGC 7 cut(s) 79, 91, 116, 139, 280, 346, 589
Bme1390I CCNGG 1 cut(s) 724
Bme18I GGWCC 1 cut(s) 705
BmgT120I GGNCC 1 cut(s) 705
BmiI GGNNCC 2 cut(s) 6, 397
BmrFI CCNGG 1 cut(s) 724
BmsI GCATC 5 cut(s) 139, 211, 460, 490, 512
BpmI CTGGAG 1 cut(s) 621
Bsa29I ATCGAT 1 cut(s) 730
BsaJI CCNNGG 1 cut(s) 326
BsaXI ACNNNNNCTCC 2 cut(s) 701, 731
Bse1I ACTGG 1 cut(s) 138
Bse3DI GCAATG 3 cut(s) 19, 225, 255
BseBI CCWGG 1 cut(s) 724
BseCI ATCGAT 1 cut(s) 730
BseDI CCNNGG 1 cut(s) 326
BseGI GGATG 3 cut(s) 220, 226, 527
BseMI GCAATG 3 cut(s) 19, 225, 255
BseNI ACTGG 1 cut(s) 138
BseRI GAGGAG 1 cut(s) 78
BseXI GCAGC 5 cut(s) 64, 101, 101, 124, 265
BshFI GGCC 1 cut(s) 665
BshVI ATCGAT 1 cut(s) 730
BsiHKAI GWGCWC 1 cut(s) 607
BsnI GGCC 1 cut(s) 665
Bsp1286I GDGCHC 1 cut(s) 607
Bsp143I GATC 5 cut(s) 4, 121, 171, 199, 358
Bsp19I CCATGG 1 cut(s) 326
BspACI CCGC 2 cut(s) 344, 587
BspANI GGCC 1 cut(s) 665
BspDI ATCGAT 1 cut(s) 730
BspLI GGNNCC 2 cut(s) 6, 397
BspMAI CTGCAG 2 cut(s) 283, 496
BspPI GGATC 3 cut(s) 12, 194, 366
BsrBI CCGCTC 1 cut(s) 587
BsrDI GCAATG 3 cut(s) 19, 225, 255
BsrI ACTGG 1 cut(s) 138
BssECI CCNNGG 1 cut(s) 326
BssMI GATC 5 cut(s) 4, 121, 171, 199, 358
BssT1I CCWWGG 1 cut(s) 326
Bst2UI CCWGG 1 cut(s) 724
Bst4CI ACNGT 2 cut(s) 53, 340
Bst6I CTCTTC 1 cut(s) 681
BstAPI GCANNNNNTGC 3 cut(s) 86, 236, 500
BstC8I GCNNGC 1 cut(s) 283
BstDSI CCRYGG 1 cut(s) 326
BstF5I GGATG 3 cut(s) 220, 226, 527
BstKTI GATC 5 cut(s) 7, 124, 174, 202, 361
BstMBI GATC 5 cut(s) 4, 121, 171, 199, 358
BstMWI GCNNNNNNNGC 4 cut(s) 86, 236, 245, 500
BstNI CCWGG 1 cut(s) 724
BstSCI CCNGG 1 cut(s) 722
BstSFI CTRYAG 2 cut(s) 279, 492
BstV1I GCAGC 5 cut(s) 64, 101, 101, 124, 265
BstX2I RGATCY 2 cut(s) 4, 199
BstYI RGATCY 2 cut(s) 4, 199
Bsu15I ATCGAT 1 cut(s) 730
BsuRI GGCC 1 cut(s) 665
BsuTUI ATCGAT 1 cut(s) 730
BtgI CCRYGG 1 cut(s) 326
BtgZI GCGATG 1 cut(s) 579
BtsCI GGATG 3 cut(s) 220, 226, 527
BtsI GCAGTG 1 cut(s) 489
BtsIMutI CAGTG 1 cut(s) 489
Cac8I GCNNGC 1 cut(s) 283
Cfr13I GGNCC 1 cut(s) 705
ClaI ATCGAT 1 cut(s) 730
CsiI ACCWGGT 1 cut(s) 722
Csp6I GTAC 1 cut(s) 54
CspCI CAANNNNNGTGG 2 cut(s) 388, 423
CviAII CATG 4 cut(s) 245, 327, 355, 712
CviJI RGCY 3 cut(s) 137, 278, 665
CviKI_1 RGCY 3 cut(s) 137, 278, 665
CviQI GTAC 1 cut(s) 54
DpnI GATC 5 cut(s) 6, 123, 173, 201, 360
DpnII GATC 5 cut(s) 4, 121, 171, 199, 358
DraI TTTAAA 1 cut(s) 30
Eam1104I CTCTTC 1 cut(s) 681
EarI CTCTTC 1 cut(s) 681
Eco130I CCWWGG 1 cut(s) 326
Eco47I GGWCC 1 cut(s) 705
EcoO109I RGGNCCY 1 cut(s) 705
EcoRII CCWGG 1 cut(s) 722
EcoT14I CCWWGG 1 cut(s) 326
ErhI CCWWGG 1 cut(s) 326
FaeI CATG 4 cut(s) 248, 330, 358, 715
FatI CATG 4 cut(s) 244, 326, 354, 711
Fnu4HI GCNGC 7 cut(s) 78, 90, 115, 138, 279, 345, 588
FokI GGATG 3 cut(s) 227, 233, 534
Fsp4HI GCNGC 7 cut(s) 78, 90, 115, 138, 279, 345, 588
FspBI CTAG 1 cut(s) 44
GluI GCNGC 7 cut(s) 78, 90, 115, 138, 279, 345, 588
GsuI CTGGAG 1 cut(s) 621
HaeIII GGCC 1 cut(s) 665
Hin1II CATG 4 cut(s) 248, 330, 358, 715
HincII GTYRAC 1 cut(s) 373
HindII GTYRAC 1 cut(s) 373
HinfI GANTC 1 cut(s) 732
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 2 cut(s) 204, 456
Hpy188III TCNNGA 1 cut(s) 600
Hpy8I GTNNAC 1 cut(s) 373
HpyAV CCTTC 2 cut(s) 512, 676
HpyCH4III ACNGT 2 cut(s) 53, 340
HpyCH4IV ACGT 1 cut(s) 674
HpyF10VI GCNNNNNNNGC 4 cut(s) 86, 236, 245, 500
HpySE526I ACGT 1 cut(s) 674
Hsp92II CATG 4 cut(s) 248, 330, 358, 715
Kzo9I GATC 5 cut(s) 4, 121, 171, 199, 358
LmnI GCTCC 1 cut(s) 602
LpnPI CCDG 9 cut(s) 87, 119, 260, 267, 459, 585, 688, 709, 736
Lsp1109I GCAGC 5 cut(s) 64, 101, 101, 124, 265
LweI GCATC 5 cut(s) 139, 211, 460, 490, 512
MabI ACCWGGT 1 cut(s) 722
MaeI CTAG 1 cut(s) 44
MaeII ACGT 1 cut(s) 674
MaeIII GTNAC 2 cut(s) 350, 616
MalI GATC 5 cut(s) 6, 123, 173, 201, 360
MbiI CCGCTC 1 cut(s) 587
MboI GATC 5 cut(s) 4, 121, 171, 199, 358
MboII GAAGA 4 cut(s) 265, 509, 527, 698
MfeI CAATTG 1 cut(s) 231
MflI RGATCY 2 cut(s) 4, 199
MhlI GDGCHC 1 cut(s) 607
MmeI TCCRAC 1 cut(s) 613
MnlI CCTC 5 cut(s) 56, 59, 211, 462, 676
MseI TTAA 8 cut(s) 21, 29, 168, 420, 434, 543, 624, 677
MspR9I CCNGG 1 cut(s) 724
MunI CAATTG 1 cut(s) 231
MvaI CCWGG 1 cut(s) 724
MwoI GCNNNNNNNGC 4 cut(s) 86, 236, 245, 500
NcoI CCATGG 1 cut(s) 326
NdeII GATC 5 cut(s) 4, 121, 171, 199, 358
NlaIII CATG 4 cut(s) 248, 330, 358, 715
NlaIV GGNNCC 2 cut(s) 6, 397
PcsI WCGNNNNNNNCGW 1 cut(s) 736
PfeI GAWTC 1 cut(s) 732
PkrI GCNGC 7 cut(s) 79, 91, 116, 139, 280, 346, 589
PpuMI RGGWCCY 1 cut(s) 705
Psp1406I AACGTT 1 cut(s) 674
Psp5II RGGWCCY 1 cut(s) 705
Psp6I CCWGG 1 cut(s) 722
PspGI CCWGG 1 cut(s) 722
PspN4I GGNNCC 2 cut(s) 6, 397
PspPI GGNCC 1 cut(s) 705
PspPPI RGGWCCY 1 cut(s) 705
PstI CTGCAG 2 cut(s) 283, 496
PsuI RGATCY 2 cut(s) 4, 199
RsaI GTAC 1 cut(s) 55
RsaNI GTAC 1 cut(s) 54
SaqAI TTAA 8 cut(s) 21, 29, 168, 420, 434, 543, 624, 677
SatI GCNGC 7 cut(s) 78, 90, 115, 138, 279, 345, 588
Sau3AI GATC 5 cut(s) 4, 121, 171, 199, 358
Sau96I GGNCC 1 cut(s) 705
ScrFI CCNGG 1 cut(s) 724
SduI GDGCHC 1 cut(s) 607
SetI ASST 4 cut(s) 131, 677, 707, 728
SexAI ACCWGGT 1 cut(s) 722
SfaNI GCATC 5 cut(s) 139, 211, 460, 490, 512
SfcI CTRYAG 2 cut(s) 279, 492
SinI GGWCC 1 cut(s) 705
SmiI ATTTAAAT 1 cut(s) 30
SsiI CCGC 2 cut(s) 344, 587
SspI AATATT 1 cut(s) 485
SspMI CTAG 1 cut(s) 44
StyD4I CCNGG 1 cut(s) 722
StyI CCWWGG 1 cut(s) 326
SwaI ATTTAAAT 1 cut(s) 30
TaaI ACNGT 2 cut(s) 53, 340
TaiI ACGT 1 cut(s) 677
TaqI TCGA 2 cut(s) 730, 739
TauI GCSGC 2 cut(s) 347, 590
TfiI GAWTC 1 cut(s) 732
Tru1I TTAA 8 cut(s) 21, 29, 168, 420, 434, 543, 624, 677
Tru9I TTAA 8 cut(s) 21, 29, 168, 420, 434, 543, 624, 677
TscAI CASTG 1 cut(s) 496
TseI GCWGC 5 cut(s) 77, 89, 114, 137, 278
TspDTI ATGAA 6 cut(s) 233, 266, 418, 642, 695, 728
TspRI CASTG 1 cut(s) 496
VpaK11BI GGWCC 1 cut(s) 705
XapI RAATTY 1 cut(s) 31
XspI CTAG 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.