pycom03g07270

Zinc finger RNA-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0012599
Physical Location & Seq
Reverse (-)
5781105 .. 5784582
3478 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g07270.1

Sequence Viewer

Length: 714 bp
ATGGCCAACTGCTTAGAGGAATTGGATAATGCAATTGCAAAATTGCATCAAAACTTTCAGGAATTGAGGTACCACCTTGAGAAGTCGTTCTTAGAGCAGGGCAGGGCCCTGTTGAAATTTGAAGCTCAGTGGAAAGATCTTAAGAAAGATCACAGCAGAGAAAACGAGCAGCGGCAAGTTCACCTGCAACAAATGGGTTTCTCAATACCATCAAATGGGACAGATCACTATGCTTCTGCAAATCCGAATGCTGCCTCATGGAGTAACACTAGTGCTCGGCCGGCTGGAAATGCTACTCCTCGTCCTTCTGGAAACGGTACTCCGAAACTGCATTGTGAGGTATGCAATATTTATTGCGACACCAAGGATGTACTCAACAAGCATAAACAGGGAAAAAAGCACAAGATGAATTTGGATAAGTTGAAGGGCAAAAAGATCCCTGGAGAAAACAGTAAGAGGAGAGCAGCTGATCAACCTCGGGAGGATTTAGAAACTAAGAGAAGGAAGGTTCTTGAAAGCGGGGCAGCACCCGAAGCTTTAAGGACTTGCGCTTTATGTAATGTGGTCTGTCTTACTGAGACGGATTTCAATAATCATCGCTCCGGACAAAGGCATGTTGAGGCCGCTACTGCGGCGAGGAGACATGGTGCTGGAACAAGTGGAGCAAGCAGCCACTGGCAGTCGCAGTGGAGATATCAGGGTTGGAATTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-met

Protein Analysis

238

Amino Acids

26.71

Weight (kDa)

9.41

Isoelectric Point (pI)

45.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012599)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g36400 FvH4_3g36400 FvH4_3g36400
malus_domestica MD00G1013500.v1.1 MD03G1090800.v1.1
prunus_persica Prupe.6G075200_v2.0.a1
pyrus_communis pycom03g07270 pycom11g08730
rosa_chinensis RchiOBHm_Chr5g0065291
rosa_laevigata RLG00000035778
rosa_multiflora Rmu_co8470125.1_g000001
rosa_roxburghii Rroxscaffold_1G00015900
rosa_rugosa Rorug05G0371000
rosa_samantha Rh5AG428800 Rh5BG444100 Rh5CG466300 Rh5DG456200 Rh5DG456300
rosa_wichuraiana Rw5G040250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 192
Acc36I ACCTGC 1 cut(s) 192
Acc65I GGTACC 1 cut(s) 69
AccB1I GGYRCC 1 cut(s) 69
AccB7I CCANNNNNTGG 1 cut(s) 215
AccIII TCCGGA 1 cut(s) 602
AciI CCGC 4 cut(s) 172, 519, 624, 632
AclWI GGATC 1 cut(s) 430
AcoI YGGCCR 2 cut(s) 3, 278
AcsI RAATTY 3 cut(s) 116, 409, 706
AfaI GTAC 3 cut(s) 71, 319, 372
AfiI CCNNNNNNNGG 2 cut(s) 215, 609
AflII CTTAAG 1 cut(s) 140
AgsI TTSAA 5 cut(s) 115, 122, 424, 515, 589
AhlI ACTAGT 1 cut(s) 269
AjnI CCWGG 1 cut(s) 439
AluBI AGCT 3 cut(s) 125, 467, 536
AluI AGCT 3 cut(s) 125, 467, 536
Alw21I GWGCWC 1 cut(s) 277
Alw26I GTCTC 2 cut(s) 572, 634
AlwI GGATC 1 cut(s) 430
AlwNI CAGNNNCTG 1 cut(s) 675
Ama87I CYCGRG 1 cut(s) 477
Aor13HI TCCGGA 1 cut(s) 602
AoxI GGCC 4 cut(s) 3, 105, 278, 621
ApaI GGGCCC 1 cut(s) 109
ApeKI GCWGC 5 cut(s) 169, 251, 464, 524, 669
ApoI RAATTY 3 cut(s) 116, 409, 706
Asp700I GAANNNNTTC 1 cut(s) 86
Asp718I GGTACC 1 cut(s) 69
AspLEI GCGC 1 cut(s) 551
AspS9I GGNCC 2 cut(s) 105, 106
AsuHPI GGTGA 1 cut(s) 173
AvaI CYCGRG 1 cut(s) 477
BaeGI GKGCMC 1 cut(s) 109
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 69
BanII GRGCYC 1 cut(s) 109
Bbv12I GWGCWC 1 cut(s) 277
BbvI GCAGC 5 cut(s) 181, 238, 476, 536, 681
BccI CCATC 1 cut(s) 217
BciT130I CCWGG 1 cut(s) 441
BclI TGATCA 1 cut(s) 469
BcoDI GTCTC 2 cut(s) 572, 634
BcuI ACTAGT 1 cut(s) 269
BfaI CTAG 1 cut(s) 270
BfrI CTTAAG 1 cut(s) 140
BfuAI ACCTGC 1 cut(s) 192
BglII AGATCT 1 cut(s) 136
BisI GCNGC 8 cut(s) 170, 173, 252, 465, 525, 624, 633, 670
BlsI GCNGC 8 cut(s) 171, 174, 253, 466, 526, 625, 634, 671
Bme1390I CCNGG 1 cut(s) 441
BmeT110I CYCGRG 1 cut(s) 477
BmgT120I GGNCC 2 cut(s) 105, 106
BmiI GGNNCC 2 cut(s) 71, 107
BmrFI CCNGG 1 cut(s) 441
BmsI GCATC 1 cut(s) 55
BpmI CTGGAG 1 cut(s) 462
BpuEI CTTGAG 1 cut(s) 98
BsaJI CCNNGG 3 cut(s) 363, 439, 476
BsaWI WCCGGW 1 cut(s) 602
BsaXI ACNNNNNCTCC 2 cut(s) 631, 661
Bsc4I CCNNNNNNNGG 2 cut(s) 215, 609
Bse118I RCCGGY 1 cut(s) 280
Bse1I ACTGG 1 cut(s) 680
BseAI TCCGGA 1 cut(s) 602
BseBI CCWGG 1 cut(s) 441
BseDI CCNNGG 3 cut(s) 363, 439, 476
BseGI GGATG 1 cut(s) 373
BseLI CCNNNNNNNGG 2 cut(s) 215, 609
BseMII CTCAG 2 cut(s) 140, 567
BseNI ACTGG 1 cut(s) 680
BseRI GAGGAG 3 cut(s) 288, 472, 652
BseSI GKGCMC 1 cut(s) 109
BseX3I CGGCCG 1 cut(s) 278
BseXI GCAGC 5 cut(s) 181, 238, 476, 536, 681
Bsh1285I CGRYCG 1 cut(s) 281
BshFI GGCC 4 cut(s) 5, 107, 280, 623
BshNI GGYRCC 1 cut(s) 69
BsiEI CGRYCG 1 cut(s) 281
BsiHKAI GWGCWC 1 cut(s) 277
BsiHKCI CYCGRG 1 cut(s) 477
BsiSI CCGG 2 cut(s) 281, 603
BslFI GGGAC 1 cut(s) 232
BslI CCNNNNNNNGG 2 cut(s) 215, 609
BsmAI GTCTC 2 cut(s) 572, 634
BsmBI CGTCTC 1 cut(s) 572
BsmFI GGGAC 1 cut(s) 232
BsmI GAATGC 1 cut(s) 253
BsnI GGCC 4 cut(s) 5, 107, 280, 623
BsoBI CYCGRG 1 cut(s) 477
Bsp120I GGGCCC 1 cut(s) 105
Bsp1286I GDGCHC 2 cut(s) 109, 277
Bsp13I TCCGGA 1 cut(s) 602
Bsp143I GATC 5 cut(s) 136, 148, 223, 435, 469
BspACI CCGC 4 cut(s) 172, 519, 624, 632
BspANI GGCC 4 cut(s) 5, 107, 280, 623
BspCNI CTCAG 2 cut(s) 139, 568
BspEI TCCGGA 1 cut(s) 602
BspLI GGNNCC 2 cut(s) 71, 107
BspMI ACCTGC 1 cut(s) 192
BspPI GGATC 1 cut(s) 430
BspT107I GGYRCC 1 cut(s) 69
BspTI CTTAAG 1 cut(s) 140
BsrFI RCCGGY 1 cut(s) 280
BsrI ACTGG 1 cut(s) 680
BssAI RCCGGY 1 cut(s) 280
BssECI CCNNGG 3 cut(s) 363, 439, 476
BssMI GATC 5 cut(s) 136, 148, 223, 435, 469
BssT1I CCWWGG 1 cut(s) 363
Bst2UI CCWGG 1 cut(s) 441
Bst4CI ACNGT 2 cut(s) 317, 452
BstAFI CTTAAG 1 cut(s) 140
BstC8I GCNNGC 2 cut(s) 282, 667
BstDEI CTNAG 5 cut(s) 13, 91, 126, 495, 576
BstF5I GGATG 1 cut(s) 373
BstHHI GCGC 1 cut(s) 551
BstKTI GATC 5 cut(s) 139, 151, 226, 438, 472
BstMAI GTCTC 2 cut(s) 572, 634
BstMBI GATC 5 cut(s) 136, 148, 223, 435, 469
BstMCI CGRYCG 1 cut(s) 281
BstMWI GCNNNNNNNGC 5 cut(s) 281, 290, 533, 629, 632
BstNI CCWGG 1 cut(s) 441
BstNSI RCATGY 1 cut(s) 617
BstSCI CCNGG 1 cut(s) 439
BstSLI GKGCMC 1 cut(s) 109
BstV1I GCAGC 5 cut(s) 181, 238, 476, 536, 681
BstX2I RGATCY 2 cut(s) 136, 435
BstYI RGATCY 2 cut(s) 136, 435
BstZI CGGCCG 1 cut(s) 278
BsuRI GGCC 4 cut(s) 5, 107, 280, 623
BtgZI GCGATG 1 cut(s) 581
BtsCI GGATG 1 cut(s) 373
BtsI GCAGTG 1 cut(s) 692
BtsIMutI CAGTG 3 cut(s) 134, 673, 692
BveI ACCTGC 1 cut(s) 192
Cac8I GCNNGC 2 cut(s) 282, 667
CaiI CAGNNNCTG 1 cut(s) 675
CfoI GCGC 1 cut(s) 551
Cfr10I RCCGGY 1 cut(s) 280
Cfr13I GGNCC 2 cut(s) 105, 106
Csp6I GTAC 3 cut(s) 70, 318, 371
CviAII CATG 3 cut(s) 258, 614, 644
CviJI RGCY 9 cut(s) 5, 107, 125, 280, 284, 467, 536, 623, 672
CviKI_1 RGCY 9 cut(s) 5, 107, 125, 280, 284, 467, 536, 623, 672
CviQI GTAC 3 cut(s) 70, 318, 371
DdeI CTNAG 5 cut(s) 13, 91, 126, 495, 576
DpnI GATC 5 cut(s) 138, 150, 225, 437, 471
DpnII GATC 5 cut(s) 136, 148, 223, 435, 469
EaeI YGGCCR 2 cut(s) 3, 278
EagI CGGCCG 1 cut(s) 278
EclXI CGGCCG 1 cut(s) 278
Eco130I CCWWGG 1 cut(s) 363
Eco24I GRGCYC 1 cut(s) 109
Eco32I GATATC 1 cut(s) 695
Eco52I CGGCCG 1 cut(s) 278
Eco88I CYCGRG 1 cut(s) 477
EcoO109I RGGNCCY 2 cut(s) 105, 106
EcoRI GAATTC 1 cut(s) 706
EcoRII CCWGG 1 cut(s) 439
EcoRV GATATC 1 cut(s) 695
EcoT14I CCWWGG 1 cut(s) 363
EcoT38I GRGCYC 1 cut(s) 109
ErhI CCWWGG 1 cut(s) 363
Esp3I CGTCTC 1 cut(s) 572
FaeI CATG 3 cut(s) 261, 617, 647
FaiI YATR 8 cut(s) 231, 259, 343, 384, 556, 615, 645, 712
FalI AAGNNNNNCTT 2 cut(s) 74, 106
FaqI GGGAC 1 cut(s) 232
FatI CATG 3 cut(s) 257, 613, 643
FauI CCCGC 1 cut(s) 512
FbaI TGATCA 1 cut(s) 469
Fnu4HI GCNGC 8 cut(s) 170, 173, 252, 465, 525, 624, 633, 670
FokI GGATG 1 cut(s) 380
FriOI GRGCYC 1 cut(s) 109
Fsp4HI GCNGC 8 cut(s) 170, 173, 252, 465, 525, 624, 633, 670
FspBI CTAG 1 cut(s) 270
GlaI GCGC 1 cut(s) 550
GluI GCNGC 8 cut(s) 170, 173, 252, 465, 525, 624, 633, 670
GsuI CTGGAG 1 cut(s) 462
HaeIII GGCC 4 cut(s) 5, 107, 280, 623
HapII CCGG 2 cut(s) 281, 603
HhaI GCGC 1 cut(s) 551
Hin1II CATG 3 cut(s) 261, 617, 647
Hin6I GCGC 1 cut(s) 549
HinP1I GCGC 1 cut(s) 549
HindIII AAGCTT 1 cut(s) 534
HpaII CCGG 2 cut(s) 281, 603
HphI GGTGA 1 cut(s) 173
Hpy166II GTNNAC 1 cut(s) 181
Hpy188I TCNGA 2 cut(s) 246, 324
Hpy188III TCNNGA 5 cut(s) 59, 309, 479, 512, 603
Hpy8I GTNNAC 1 cut(s) 181
HpyAV CCTTC 4 cut(s) 315, 418, 495, 499
HpyCH4III ACNGT 2 cut(s) 317, 452
HpyCH4V TGCA 7 cut(s) 32, 38, 46, 187, 239, 331, 345
HpyF10VI GCNNNNNNNGC 5 cut(s) 281, 290, 533, 629, 632
HpyF3I CTNAG 5 cut(s) 13, 91, 126, 495, 576
Hsp92II CATG 3 cut(s) 261, 617, 647
HspAI GCGC 1 cut(s) 549
Kpn2I TCCGGA 1 cut(s) 602
KpnI GGTACC 1 cut(s) 73
KroI GCCGGC 1 cut(s) 280
KroNI GCCGGC 1 cut(s) 282
Ksp22I TGATCA 1 cut(s) 469
Kzo9I GATC 5 cut(s) 136, 148, 223, 435, 469
LmnI GCTCC 2 cut(s) 605, 662
Lsp1109I GCAGC 5 cut(s) 181, 238, 476, 536, 681
LweI GCATC 1 cut(s) 55
MaeI CTAG 1 cut(s) 270
MaeIII GTNAC 1 cut(s) 263
MalI GATC 5 cut(s) 138, 150, 225, 437, 471
MboI GATC 5 cut(s) 136, 148, 223, 435, 469
MfeI CAATTG 1 cut(s) 33
MflI RGATCY 2 cut(s) 136, 435
MhlI GDGCHC 2 cut(s) 109, 277
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 7 cut(s) 20, 33, 41, 62, 116, 409, 706
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 683
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 602
MroNI GCCGGC 1 cut(s) 280
MroXI GAANNNNTTC 1 cut(s) 86
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 141, 539
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 172, 467
MspCI CTTAAG 1 cut(s) 140
MspI CCGG 2 cut(s) 281, 603
MspR9I CCNGG 1 cut(s) 441
MunI CAATTG 1 cut(s) 33
Mva1269I GAATGC 1 cut(s) 253
MvaI CCWGG 1 cut(s) 441
MwoI GCNNNNNNNGC 5 cut(s) 281, 290, 533, 629, 632
NaeI GCCGGC 1 cut(s) 282
NdeII GATC 5 cut(s) 136, 148, 223, 435, 469
NgoMIV GCCGGC 1 cut(s) 280
NlaIII CATG 3 cut(s) 261, 617, 647
NlaIV GGNNCC 2 cut(s) 71, 107
NmeAIII GCCGAG 1 cut(s) 256
NspI RCATGY 1 cut(s) 617
PaqCI CACCTGC 1 cut(s) 192
PctI GAATGC 1 cut(s) 253
PdiI GCCGGC 1 cut(s) 282
PdmI GAANNNNTTC 1 cut(s) 86
PflMI CCANNNNNTGG 1 cut(s) 215
PkrI GCNGC 8 cut(s) 171, 174, 253, 466, 526, 625, 634, 671
Psp6I CCWGG 1 cut(s) 439
PspGI CCWGG 1 cut(s) 439
PspN4I GGNNCC 2 cut(s) 71, 107
PspOMI GGGCCC 1 cut(s) 105
PspPI GGNCC 2 cut(s) 105, 106
PstNI CAGNNNCTG 1 cut(s) 675
PsuI RGATCY 2 cut(s) 136, 435
PvuII CAGCTG 1 cut(s) 467
RsaI GTAC 3 cut(s) 71, 319, 372
RsaNI GTAC 3 cut(s) 70, 318, 371
SaqAI TTAA 2 cut(s) 141, 539
SatI GCNGC 8 cut(s) 170, 173, 252, 465, 525, 624, 633, 670
Sau3AI GATC 5 cut(s) 136, 148, 223, 435, 469
Sau96I GGNCC 2 cut(s) 105, 106
ScrFI CCNGG 1 cut(s) 441
SduI GDGCHC 2 cut(s) 109, 277
SetI ASST 9 cut(s) 71, 78, 127, 186, 342, 469, 478, 510, 538
SfaNI GCATC 1 cut(s) 55
SmlI CTYRAG 2 cut(s) 77, 140
SmoI CTYRAG 2 cut(s) 77, 140
SpeI ACTAGT 1 cut(s) 269
Sse9I AATT 7 cut(s) 20, 33, 41, 62, 116, 409, 706
SsiI CCGC 4 cut(s) 172, 519, 624, 632
SspI AATATT 1 cut(s) 349
SspMI CTAG 1 cut(s) 270
StyD4I CCNGG 1 cut(s) 439
StyI CCWWGG 1 cut(s) 363
TaaI ACNGT 2 cut(s) 317, 452
TasI AATT 7 cut(s) 20, 33, 41, 62, 116, 409, 706
TatI WGTACW 1 cut(s) 370
TauI GCSGC 3 cut(s) 175, 626, 635
Tru1I TTAA 2 cut(s) 141, 539
Tru9I TTAA 2 cut(s) 141, 539
TscAI CASTG 3 cut(s) 134, 680, 692
TseI GCWGC 5 cut(s) 169, 251, 464, 524, 669
TspDTI ATGAA 2 cut(s) 422, 699
TspGWI ACGGA 1 cut(s) 596
TspRI CASTG 3 cut(s) 134, 680, 692
Van91I CCANNNNNTGG 1 cut(s) 215
Vha464I CTTAAG 1 cut(s) 140
XapI RAATTY 3 cut(s) 116, 409, 706
XceI RCATGY 1 cut(s) 617
XmnI GAANNNNTTC 1 cut(s) 86
XspI CTAG 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.