pycom03g17490

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0004980
Physical Location & Seq
Reverse (-)
18612832 .. 18616411
3580 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1659 bp
ATGGATGTTGATGAGTATGCCCAATTGCAAGCCCATTTTGATAATGTGGATATTCCCCCTGGAATAGAGGCACCTATTCCTTGGCTGTCGGATCCTCCTAAAAATAAAGTTAATTCAGTTTCTGGAAGTAGTTCCGTCAATAAGAGGTTTCAAATGCAACAAGAGCCTGATCATTTCATTAAGATGCCACTTTTTGGGGGTAGTTCAAGCTTGAAGACCCAAATTGATAGTATTTCCCACCCACCTGAAGTGAATCTATCATCTGCATGGAAACTTCCAAAAACTGCCAGGAGTAAAAAGAAGCAACCTGCTTCACAACACCAAGGGAGTGCTCCTAATCTCCCAGTTGGGAAAGAGTCATCAAAGTCTCAATGGCTTTTAGGACCTTTTCAGTCTAAAAAGAAGGTAGCTTCTTCGAGTAGTTCGACGAATCAATTTGATGCAATGAAGCTGGGTTCCGGAGCTGACACATCTTCTGCTTCGTATTTTCATAACATTCTAAAGAAAAAGGGTTCAAGTGCTTTTTATAAACCCATGCCCGTTCCTGGGTGGCCCGGGCCCATTTCCAAATTTAATACCATGCCATTTAATTCAAGCTTGTACGATCTAGATTCATTATATTCTCCTGGGGAAGTAGCAGGTAGTCCTTGGATTGCTAATGCGCAAATTCAAAATAATCTTGCTCCAGGAGGCATTTCAACAAGCCCTGGGAGGAAAATTTCTGCCATGGAAATGGATGAAATGATAGTGAAGTTTAAGGGCTTCAAAAAATTTGATACAGTTGAAGATCATTCAGACCATCACTATAGTAGGTGTGGCTATTCACCGAAGCAACCACCAAAGAGTTGGGCAAAGAGAATTCAGGAAGAGTGGAAGATCCTGGAGAAGGATTTGCCTGATACAATATTTGTTAGAGCTTATGAGACAAGAATGGATCTTTTGAGAGCTGTAATTATTGGGGCGGAGGGTACTCCTTACCATGATGGTCTCTTCTTCTTTGATGTTTGCTTCCCCTGTGGCTATCCCAATGTACCACCGAATGTCTACTACCACTCTGGTGGCCTTCGATTAAATCCAAATTTGTACAATTGTGGAAAAGTATGCCTCAGCCTTCGTAACACCTGGTCTGGGAACAAGAATGAGAAGTGGCTCCCAGGTGTGTCAACCATGCTACAAGTGTTGGTCTCTATACAGGGGCTGATCTTGAATACAAAGCCCTACTTTAATGAGCCTGGATATGCAAATACGAATGGTTCAGCAGCTGGTGAAAAGAGGTCCGAGGAGTACAATGAGAATACATTCATCCTATCATTGAAGACAATGGTGTACAGCATGAGGAGGCCTCCGAAGCATTTTGAGGATCTTGTTCTGGGGCATTTCTACAATCGTGCTCGTGACATTCTGGTGGCATGCAAAGCATACATGGATGGTGCTCAAGTGGGGTGTCTAGTCAAAGGTGGTGTTCAAGATGTTGATGAGGGCGACAAGAGCTGCTCACAGCGTTTCAAAACTTCTGTAGCCGACCATGTGCCTATGATTGTCGCAGAGTTTACAAGAATCGGGGTCAAGGATTGTGAGAGATTCGTATCACCAGCAACATCTGGGAACAACCAAATTGCCAGTATGCCTCAGGCTGTAACGTCAATGATTTCATGCTGA

Protein Analysis

553

Amino Acids

61.06

Weight (kDa)

8.83

Isoelectric Point (pI)

48.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 286 - 431 5.5e-19 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 528
Acc16I TGCGCA 1 cut(s) 663
Acc36I ACCTGC 2 cut(s) 316, 629
AccB1I GGYRCC 1 cut(s) 70
AccB7I CCANNNNNTGG 1 cut(s) 194
AccI GTMKAC 1 cut(s) 1044
AccIII TCCGGA 1 cut(s) 458
AciI CCGC 1 cut(s) 962
AclWI GGATC 5 cut(s) 86, 99, 871, 942, 1368
AcsI RAATTY 6 cut(s) 569, 666, 717, 770, 858, 1078
AcuI CTGAAG 1 cut(s) 267
AfaI GTAC 6 cut(s) 602, 970, 1032, 1085, 1286, 1328
AfiI CCNNNNNNNGG 5 cut(s) 194, 545, 546, 886, 1128
AleI CACNNNNGTG 1 cut(s) 1056
AluBI AGCT 9 cut(s) 210, 410, 451, 464, 597, 917, 947, 1262, 1491
AluI AGCT 9 cut(s) 210, 410, 451, 464, 597, 917, 947, 1262, 1491
Alw21I GWGCWC 3 cut(s) 334, 1393, 1435
Alw26I GTCTC 4 cut(s) 372, 917, 992, 1189
AlwI GGATC 5 cut(s) 86, 99, 871, 942, 1368
AlwNI CAGNNNCTG 3 cut(s) 122, 1198, 1262
Ama87I CYCGRG 1 cut(s) 554
Aor13HI TCCGGA 1 cut(s) 458
AoxI GGCC 4 cut(s) 551, 557, 1060, 1340
ApaI GGGCCC 1 cut(s) 561
ApeKI GCWGC 2 cut(s) 1259, 1491
ApoI RAATTY 6 cut(s) 569, 666, 717, 770, 858, 1078
Asp700I GAANNNNTTC 2 cut(s) 130, 1298
AspLEI GCGC 1 cut(s) 664
AspS9I GGNCC 5 cut(s) 383, 552, 557, 558, 1275
AsuC2I CCSGG 2 cut(s) 555, 556
AsuHPI GGTGA 3 cut(s) 816, 1277, 1581
AvaI CYCGRG 1 cut(s) 554
AvaII GGWCC 2 cut(s) 383, 1275
AxyI CCTNAGG 1 cut(s) 1629
BaeGI GKGCMC 1 cut(s) 561
BamHI GGATCC 1 cut(s) 91
BanI GGYRCC 1 cut(s) 70
BanII GRGCYC 1 cut(s) 561
BauI CACGAG 1 cut(s) 1392
BbsI GAAGAC 2 cut(s) 221, 1322
Bbv12I GWGCWC 3 cut(s) 334, 1393, 1435
BbvCI CCTCAGC 1 cut(s) 1106
BbvI GCAGC 2 cut(s) 1271, 1478
BccI CCATC 3 cut(s) 807, 977, 1421
BclI TGATCA 1 cut(s) 169
BcnI CCSGG 2 cut(s) 555, 556
BcoDI GTCTC 4 cut(s) 372, 917, 992, 1189
BfaI CTAG 2 cut(s) 608, 1448
BfmI CTRYAG 2 cut(s) 805, 1515
BfuAI ACCTGC 2 cut(s) 316, 629
BisI GCNGC 2 cut(s) 1260, 1492
BlsI GCNGC 2 cut(s) 1261, 1493
Bme18I GGWCC 2 cut(s) 383, 1275
BmeT110I CYCGRG 1 cut(s) 554
BmgT120I GGNCC 5 cut(s) 383, 552, 557, 558, 1275
BmiI GGNNCC 5 cut(s) 72, 93, 457, 559, 1151
BmrI ACTGGG 1 cut(s) 338
BmsI GCATC 2 cut(s) 174, 430
BmuI ACTGGG 1 cut(s) 338
BpiI GAAGAC 2 cut(s) 221, 1322
BplI GAGNNNNNCTC 2 cut(s) 1327, 1359
BpmI CTGGAG 2 cut(s) 669, 902
Bpu10I CCTNAGC 1 cut(s) 1106
BpuEI CTTGAG 1 cut(s) 1419
BpuMI CCSGG 2 cut(s) 555, 556
BsaBI GATNNNNATC 1 cut(s) 1585
BsaI GGTCTC 2 cut(s) 992, 1189
BsaWI WCCGGW 1 cut(s) 458
Bsc4I CCNNNNNNNGG 5 cut(s) 194, 545, 546, 886, 1128
Bse1I ACTGG 2 cut(s) 344, 1620
Bse21I CCTNAGG 1 cut(s) 1629
Bse3DI GCAATG 1 cut(s) 450
Bse8I GATNNNNATC 1 cut(s) 1585
BseAI TCCGGA 1 cut(s) 458
BseGI GGATG 4 cut(s) 10, 742, 1302, 1432
BseJI GATNNNNATC 1 cut(s) 1585
BseLI CCNNNNNNNGG 5 cut(s) 194, 545, 546, 886, 1128
BseMI GCAATG 1 cut(s) 450
BseMII CTCAG 2 cut(s) 1120, 1643
BseNI ACTGG 2 cut(s) 344, 1620
BseRI GAGGAG 2 cut(s) 1295, 1351
BseSI GKGCMC 1 cut(s) 561
BseXI GCAGC 2 cut(s) 1271, 1478
BseYI CCCAGC 1 cut(s) 451
BshFI GGCC 4 cut(s) 553, 559, 1062, 1342
BshNI GGYRCC 1 cut(s) 70
BsiHKAI GWGCWC 3 cut(s) 334, 1393, 1435
BsiHKCI CYCGRG 1 cut(s) 554
BsiSI CCGG 2 cut(s) 459, 555
BslI CCNNNNNNNGG 5 cut(s) 194, 545, 546, 886, 1128
BsmAI GTCTC 4 cut(s) 372, 917, 992, 1189
BsnI GGCC 4 cut(s) 553, 559, 1062, 1342
Bso31I GGTCTC 2 cut(s) 992, 1189
BsoBI CYCGRG 1 cut(s) 554
Bsp120I GGGCCC 1 cut(s) 557
Bsp1286I GDGCHC 4 cut(s) 334, 561, 1393, 1435
Bsp13I TCCGGA 1 cut(s) 458
Bsp1407I TGTACA 2 cut(s) 1083, 1326
Bsp143I GATC 8 cut(s) 91, 169, 604, 787, 876, 934, 1200, 1360
Bsp19I CCATGG 1 cut(s) 726
BspACI CCGC 1 cut(s) 962
BspANI GGCC 4 cut(s) 553, 559, 1062, 1342
BspCNI CTCAG 2 cut(s) 1119, 1642
BspEI TCCGGA 1 cut(s) 458
BspLI GGNNCC 5 cut(s) 72, 93, 457, 559, 1151
BspMI ACCTGC 2 cut(s) 316, 629
BspPI GGATC 5 cut(s) 86, 99, 871, 942, 1368
BspT107I GGYRCC 1 cut(s) 70
BspTNI GGTCTC 2 cut(s) 992, 1189
BsrDI GCAATG 1 cut(s) 450
BsrGI TGTACA 2 cut(s) 1083, 1326
BsrI ACTGG 2 cut(s) 344, 1620
BssMI GATC 8 cut(s) 91, 169, 604, 787, 876, 934, 1200, 1360
BssSI CACGAG 1 cut(s) 1392
BssT1I CCWWGG 4 cut(s) 80, 322, 647, 726
Bst2BI CACGAG 1 cut(s) 1392
Bst4CI ACNGT 1 cut(s) 781
Bst6I CTCTTC 2 cut(s) 861, 995
BstAUI TGTACA 2 cut(s) 1083, 1326
BstC8I GCNNGC 2 cut(s) 30, 1411
BstDEI CTNAG 2 cut(s) 1106, 1629
BstDSI CCRYGG 1 cut(s) 726
BstENI CCTNNNNNAGG 1 cut(s) 884
BstF5I GGATG 4 cut(s) 10, 742, 1302, 1432
BstHHI GCGC 1 cut(s) 664
BstKTI GATC 8 cut(s) 94, 172, 607, 790, 879, 937, 1203, 1363
BstMAI GTCTC 4 cut(s) 372, 917, 992, 1189
BstMBI GATC 8 cut(s) 91, 169, 604, 787, 876, 934, 1200, 1360
BstMWI GCNNNNNNNGC 4 cut(s) 163, 1348, 1415, 1488
BstNSI RCATGY 1 cut(s) 1413
BstSFI CTRYAG 2 cut(s) 805, 1515
BstSLI GKGCMC 1 cut(s) 561
BstV1I GCAGC 2 cut(s) 1271, 1478
BstV2I GAAGAC 2 cut(s) 221, 1322
BstX2I RGATCY 4 cut(s) 91, 876, 934, 1360
BstXI CCANNNNNNTGG 3 cut(s) 733, 846, 1058
BstYI RGATCY 4 cut(s) 91, 876, 934, 1360
Bsu36I CCTNAGG 1 cut(s) 1629
BsuRI GGCC 4 cut(s) 553, 559, 1062, 1342
BtgI CCRYGG 1 cut(s) 726
BtsCI GGATG 4 cut(s) 10, 742, 1302, 1432
BveI ACCTGC 2 cut(s) 316, 629
Cac8I GCNNGC 2 cut(s) 30, 1411
CaiI CAGNNNCTG 3 cut(s) 122, 1198, 1262
CfoI GCGC 1 cut(s) 664
Cfr13I GGNCC 5 cut(s) 383, 552, 557, 558, 1275
Cfr9I CCCGGG 1 cut(s) 554
CsiI ACCWGGT 1 cut(s) 1121
Csp6I GTAC 6 cut(s) 601, 969, 1031, 1084, 1285, 1327
CviQI GTAC 6 cut(s) 601, 969, 1031, 1084, 1285, 1327
DdeI CTNAG 2 cut(s) 1106, 1629
DpnI GATC 8 cut(s) 93, 171, 606, 789, 878, 936, 1202, 1362
DpnII GATC 8 cut(s) 91, 169, 604, 787, 876, 934, 1200, 1360
Eam1104I CTCTTC 2 cut(s) 861, 995
EarI CTCTTC 2 cut(s) 861, 995
EciI GGCGGA 1 cut(s) 977
Eco130I CCWWGG 4 cut(s) 80, 322, 647, 726
Eco147I AGGCCT 1 cut(s) 1342
Eco24I GRGCYC 1 cut(s) 561
Eco31I GGTCTC 2 cut(s) 992, 1189
Eco47I GGWCC 2 cut(s) 383, 1275
Eco57I CTGAAG 1 cut(s) 267
Eco81I CCTNAGG 1 cut(s) 1629
Eco88I CYCGRG 1 cut(s) 554
EcoNI CCTNNNNNAGG 1 cut(s) 884
EcoO109I RGGNCCY 1 cut(s) 383
EcoRI GAATTC 1 cut(s) 858
EcoT14I CCWWGG 4 cut(s) 80, 322, 647, 726
EcoT38I GRGCYC 1 cut(s) 561
ErhI CCWWGG 4 cut(s) 80, 322, 647, 726
FalI AAGNNNNNCTT 2 cut(s) 1205, 1237
FbaI TGATCA 1 cut(s) 169
FblI GTMKAC 1 cut(s) 1044
Fnu4HI GCNGC 2 cut(s) 1260, 1492
FokI GGATG 4 cut(s) 17, 749, 1289, 1439
FriOI GRGCYC 1 cut(s) 561
Fsp4HI GCNGC 2 cut(s) 1260, 1492
FspBI CTAG 2 cut(s) 608, 1448
FspI TGCGCA 1 cut(s) 663
GlaI GCGC 1 cut(s) 663
GluI GCNGC 2 cut(s) 1260, 1492
GsaI CCCAGC 1 cut(s) 455
GsuI CTGGAG 2 cut(s) 669, 902
HaeIII GGCC 4 cut(s) 553, 559, 1062, 1342
HapII CCGG 2 cut(s) 459, 555
HhaI GCGC 1 cut(s) 664
Hin6I GCGC 1 cut(s) 662
HinP1I GCGC 1 cut(s) 662
HincII GTYRAC 1 cut(s) 1164
HindII GTYRAC 1 cut(s) 1164
HindIII AAGCTT 2 cut(s) 208, 595
HinfI GANTC 6 cut(s) 253, 356, 430, 611, 1557, 1581
HpaII CCGG 2 cut(s) 459, 555
HphI GGTGA 3 cut(s) 816, 1277, 1581
Hpy166II GTNNAC 4 cut(s) 1045, 1164, 1327, 1551
Hpy188I TCNGA 4 cut(s) 91, 796, 1279, 1347
Hpy188III TCNNGA 7 cut(s) 123, 459, 608, 863, 1204, 1394, 1466
Hpy8I GTNNAC 4 cut(s) 1045, 1164, 1327, 1551
Hpy99I CGWCG 1 cut(s) 430
HpyAV CCTTC 4 cut(s) 397, 880, 1073, 1121
HpyCH4III ACNGT 1 cut(s) 781
HpyCH4IV ACGT 1 cut(s) 1640
HpyCH4V TGCA 6 cut(s) 28, 157, 266, 443, 1241, 1413
HpyF10VI GCNNNNNNNGC 4 cut(s) 163, 1348, 1415, 1488
HpyF3I CTNAG 2 cut(s) 1106, 1629
HpySE526I ACGT 1 cut(s) 1640
HspAI GCGC 1 cut(s) 662
Kpn2I TCCGGA 1 cut(s) 458
Ksp22I TGATCA 1 cut(s) 169
Kzo9I GATC 8 cut(s) 91, 169, 604, 787, 876, 934, 1200, 1360
LmnI GCTCC 4 cut(s) 337, 461, 688, 1155
Lsp1109I GCAGC 2 cut(s) 1271, 1478
LweI GCATC 2 cut(s) 174, 430
MabI ACCWGGT 1 cut(s) 1121
MaeI CTAG 2 cut(s) 608, 1448
MaeII ACGT 1 cut(s) 1640
MaeIII GTNAC 3 cut(s) 1115, 1394, 1636
MalI GATC 8 cut(s) 93, 171, 606, 789, 878, 936, 1202, 1362
MboI GATC 8 cut(s) 91, 169, 604, 787, 876, 934, 1200, 1360
MboII GAAGA 9 cut(s) 226, 405, 465, 797, 878, 886, 982, 985, 1327
MfeI CAATTG 2 cut(s) 23, 1087
MflI RGATCY 4 cut(s) 91, 876, 934, 1360
MhlI GDGCHC 4 cut(s) 334, 561, 1393, 1435
MlyI GAGTC 1 cut(s) 365
MmeI TCCRAC 1 cut(s) 69
MroI TCCGGA 1 cut(s) 458
MroXI GAANNNNTTC 2 cut(s) 130, 1298
MseI TTAA 7 cut(s) 111, 180, 573, 588, 756, 1070, 1224
MslI CAYNNNNRTG 5 cut(s) 182, 265, 731, 1056, 1403
MspA1I CMGCKG 1 cut(s) 1262
MspI CCGG 2 cut(s) 459, 555
MunI CAATTG 2 cut(s) 23, 1087
MwoI GCNNNNNNNGC 4 cut(s) 163, 1348, 1415, 1488
NciI CCSGG 2 cut(s) 555, 556
NcoI CCATGG 1 cut(s) 726
NdeII GATC 8 cut(s) 91, 169, 604, 787, 876, 934, 1200, 1360
NlaIV GGNNCC 5 cut(s) 72, 93, 457, 559, 1151
NmuCI GTSAC 1 cut(s) 1394
NsbI TGCGCA 1 cut(s) 663
NspI RCATGY 1 cut(s) 1413
OliI CACNNNNGTG 1 cut(s) 1056
PaeI GCATGC 1 cut(s) 1413
PasI CCCWGGG 1 cut(s) 707
PceI AGGCCT 1 cut(s) 1342
PcsI WCGNNNNNNNCGW 1 cut(s) 422
PdmI GAANNNNTTC 2 cut(s) 130, 1298
PfeI GAWTC 5 cut(s) 253, 430, 611, 1557, 1581
PflMI CCANNNNNTGG 1 cut(s) 194
PfoI TCCNGGA 2 cut(s) 685, 879
PkrI GCNGC 2 cut(s) 1261, 1493
PleI GAGTC 1 cut(s) 364
PpsI GAGTC 1 cut(s) 364
PpuMI RGGWCCY 1 cut(s) 383
PsiI TTATAA 1 cut(s) 528
Psp5II RGGWCCY 1 cut(s) 383
PspFI CCCAGC 1 cut(s) 451
PspN4I GGNNCC 5 cut(s) 72, 93, 457, 559, 1151
PspOMI GGGCCC 1 cut(s) 557
PspPI GGNCC 5 cut(s) 383, 552, 557, 558, 1275
PspPPI RGGWCCY 1 cut(s) 383
PstNI CAGNNNCTG 3 cut(s) 122, 1198, 1262
PsuI RGATCY 4 cut(s) 91, 876, 934, 1360
PvuII CAGCTG 1 cut(s) 1262
RsaI GTAC 6 cut(s) 602, 970, 1032, 1085, 1286, 1328
RsaNI GTAC 6 cut(s) 601, 969, 1031, 1084, 1285, 1327
RseI CAYNNNNRTG 5 cut(s) 182, 265, 731, 1056, 1403
SaqAI TTAA 7 cut(s) 111, 180, 573, 588, 756, 1070, 1224
SatI GCNGC 2 cut(s) 1260, 1492
Sau3AI GATC 8 cut(s) 91, 169, 604, 787, 876, 934, 1200, 1360
Sau96I GGNCC 5 cut(s) 383, 552, 557, 558, 1275
SchI GAGTC 1 cut(s) 365
SduI GDGCHC 4 cut(s) 334, 561, 1393, 1435
SexAI ACCWGGT 1 cut(s) 1121
SfaNI GCATC 2 cut(s) 174, 430
SfcI CTRYAG 2 cut(s) 805, 1515
SinI GGWCC 2 cut(s) 383, 1275
SmaI CCCGGG 1 cut(s) 556
SmiMI CAYNNNNRTG 5 cut(s) 182, 265, 731, 1056, 1403
SmlI CTYRAG 1 cut(s) 1434
SmoI CTYRAG 1 cut(s) 1434
SphI GCATGC 1 cut(s) 1413
SrfI GCCCGGGC 1 cut(s) 556
SseBI AGGCCT 1 cut(s) 1342
SsiI CCGC 1 cut(s) 962
SspI AATATT 1 cut(s) 906
SspMI CTAG 2 cut(s) 608, 1448
StuI AGGCCT 1 cut(s) 1342
StyI CCWWGG 4 cut(s) 80, 322, 647, 726
TaaI ACNGT 1 cut(s) 781
TaiI ACGT 1 cut(s) 1643
TaqI TCGA 3 cut(s) 416, 425, 1066
TatI WGTACW 3 cut(s) 1083, 1284, 1326
TfiI GAWTC 5 cut(s) 253, 430, 611, 1557, 1581
Tru1I TTAA 7 cut(s) 111, 180, 573, 588, 756, 1070, 1224
Tru9I TTAA 7 cut(s) 111, 180, 573, 588, 756, 1070, 1224
TseFI GTSAC 1 cut(s) 1394
TseI GCWGC 2 cut(s) 1259, 1491
Tsp45I GTSAC 1 cut(s) 1394
TspDTI ATGAA 7 cut(s) 166, 461, 479, 603, 753, 1291, 1641
TspGWI ACGGA 1 cut(s) 124
TspMI CCCGGG 1 cut(s) 554
Van91I CCANNNNNTGG 1 cut(s) 194
VpaK11BI GGWCC 2 cut(s) 383, 1275
XagI CCTNNNNNAGG 1 cut(s) 884
XapI RAATTY 6 cut(s) 569, 666, 717, 770, 858, 1078
XbaI TCTAGA 1 cut(s) 607
XceI RCATGY 1 cut(s) 1413
XcmI CCANNNNNNNNNTGG 1 cut(s) 843
XmaI CCCGGG 1 cut(s) 554
XmiI GTMKAC 1 cut(s) 1044
XmnI GAANNNNTTC 2 cut(s) 130, 1298
XspI CTAG 2 cut(s) 608, 1448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.