Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5C

Orthogroup ID

OG0004980

Physical Location & Seq

Genomic Coordinates Forward (+)

28183544 .. 28189210

Gene Structure 5667 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5CG264200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1950 bp

ATGCGATATATATTTTGTGCAGCTATATCATTCTCAAGTGTATTTACTTTATATAATTACTATTACTTTCAGGAAGCTGTCCTCCATACTTCTGTTGGTTCTTCTGGTATGGAAACCCTTGGCTCAGTCAATGGAATTGAAATTGCTAAGGATGTCGCCCCAGCATCAAATCACTTTATTGACTTGGATGGTCCCAATTCTGATGCATCTGCTGACGATGTTGATGACTGTACTGATACTTGTTTTGAAGAGTTTGATTATGCCTTCTTGCAATCCCATTTCGATAATGTGGGTATTCCTCCTGGAGTAGAGGCGCCTGTCCCTCATATTCCTTGGTTGTCAGATCCTCCTAAAAGTAATGTAAGTTCAGTTTCTGGAAGTAGTTCTGTTGATACAAGGTATCAAATGAAATCAGATTATGTTGGCCTCCATGGAAATGAGCTGTTTCCCTGGACGGATCCTCCTAGTTTCAACTTTAAGCCAACCGTTGTGGGTAGTTCAAGCTTGAAGACCCAAACAGACAGTATCGGTCACCCAAATGGACAGTACCAGTCATCTTGGAAACTTCCAAAAGCTGCTAGAAGCAGAAAGAAGCAATCTGCTTCACATCCCCAAGCTAGTGCTCCTAGTCTCCCAGTTGGAGCAGAACCATCAAAATCTCAAAGGCTCTTAGGAGCTTTGCAGCGTAAAAAGAAGCTATTTTCTTCAAGTAGTTCGACTAATTATGATGCAATGAAGTTCGATACTGGAGCCGAGACATCTTTTTTTGGACATAATTTACCCTACAGTCCAATTTATCCTTATGGATTACAGTCACCCTGGTTGTCAGATTCTTTGTCAGCAATTAGTGCTAAACCAAATCATTCAAGTTTCTACGGTCCAATTGGTTCCATATACCCTCCTGGAGAGTTAGTAGGTATCCCTTGGGTTAAGGGTGTTTCCCAAACTCAACACAATGTCACTGCTGCAAGCCTTTCAACAACCCCTGCTAGACAATTGTCTAGCAAGGAAATAGATGAAATTATGCAGAAGTACAAGGGTTTTAAACAATTTGATACAGTTGAAGATCATTCAGACCATCACTATATACTCTCTGGTACACTCGACGGTAATTCAATGACTCAGCCACCAAAGAACTGGGCAAAGAGAATTCAGGAAGAGTGGAAGAGCCTGGAGAAAGATTTGCCTGACACAGTATTTGTTAGAGTCTATGAGACAAGGATGGATCTTTTGAGAGCTGTAATTGTTGGAGCTGAGGGTACTCCTTACCATGATGGTCTCTTTTTCTTTGATGTTTCCTTCCCCAGTGGTTACCCCAATATGCCACCGCATGTCCACTACCACTCGGGTGGCCTTCGACTAAATCCAAATTTGTATGGTTGTGGAAAAGTATGCCTCAGCCTTCTCAACACCTGGTCTGGGAACTCGAAAGAGATGTGGATCCCGGGTGTGTCCACCATTCTACAAGTTTTAGTGTCCATACAAGGGCTGATCTTGAATACAAAGCCCTACTTTAATGAGCCTGGATATGCATCTATGAATGGTTCAGCAGCTGGTGAAACAAGGTCCCGGCAGTATAATGAGGATACCTTCCTCCTATCTTTAACAACGATGGTATACATGATGAGGAGGCCTCCGAAGCATTTTGAGGATTTTGTTGTGGGGCATTTCCGGGATCGTGCTCATGACATTCTGGTGGCATGTAAAGCATACATGGATGGTGCTCAAGTGGGATGTCTGGTCAAAGGTGGGGTTCAGGATGTTGATGAGGGTGACAAGAGTTGTTCTCAGCGGTTTAAGGATTCTTTAGCAGGGCACATGCCGATGCTTGTCAAAGAGTTTACACATATAGGTGTCAAGGATTGCGAGAAATATGTATCAGCAGCTGTAAATGGGAACGAGCAGATTGGTAGTATGCCGCAGGCTGCAACATCAATGGTTTCATGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0031625 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044389 GO:0044424 GO:0044464 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

UQ_con

Protein Analysis

649

Amino Acids

71.39

Weight (kDa)

5.99

Isoelectric Point (pI)

40.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
UQ_con	PF00179	383 - 504	4.8e-22	Ubiquitin-conjugating enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004980)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G15355
fragaria_vesca	FvH4_3g20000 FvH4_3g20000 FvH4_3g20000 FvH4_3g20000 FvH4_3g20000 FvH4_3g20000 FvH4_3g20000
malus_domestica	MD03G1227200.v1.1 MD11G1246700.v1.1
prunus_persica	Prupe.4G181600_v2.0.a1 Prupe.4G181600_v2.0.a1 Prupe.4G181600_v2.0.a1
pyrus_communis	pycom03g17490 pycom11g21880
rosa_chinensis	RchiOBHm_Chr5g0033831
rosa_laevigata	RLG00000033504
rosa_multiflora	Rmu_co8216886.1_g000001 Rmu_co8329207.1_g000001 Rmu_sc0001768.1_g000061 Rmu_sc0008210.1_g000001
rosa_roxburghii	Rroxscaffold_1G00046350
rosa_rugosa	Rorug05G0142600 Rorug05G0142700.1 Rorug05G0142800
rosa_samantha	Rh5AG233600 Rh5BG234100 Rh5CG264200 Rh5DG240900
rosa_wichuraiana	Rw5G021560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	313
AccI	GTMKAC	1 cut(s)	1617
AciI	CCGC	3 cut(s)	1328, 1792, 1919
AclWI	GGATC	7 cut(s)	338, 452, 465, 1233, 1435, 1448, 1683
AcsI	RAATTY	2 cut(s)	1149, 1369
AcyI	GRCGYC	1 cut(s)	314
AfaI	GTAC	5 cut(s)	232, 548, 1034, 1099, 1261
AfiI	CCNNNNNNNGG	2 cut(s)	1419, 1485
AjnI	CCWGG	7 cut(s)	301, 449, 818, 901, 1170, 1412, 1522
AleI	CACNNNNGTG	1 cut(s)	1347
Alw21I	GWGCWC	3 cut(s)	625, 1684, 1726
Alw26I	GTCTC	4 cut(s)	635, 749, 1208, 1283
AlwI	GGATC	7 cut(s)	338, 452, 465, 1233, 1435, 1448, 1683
AlwNI	CAGNNNCTG	3 cut(s)	374, 1553, 1886
Ama87I	CYCGRG	2 cut(s)	1345, 1444
AoxI	GGCC	3 cut(s)	424, 1351, 1631
ApeKI	GCWGC	7 cut(s)	20, 575, 682, 965, 1550, 1883, 1925
ApoI	RAATTY	2 cut(s)	1149, 1369
ArsI	GACNNNNNNTTYG	2 cut(s)	1181, 1213
Asp700I	GAANNNNTTC	1 cut(s)	382
AspLEI	GCGC	1 cut(s)	316
AspS9I	GGNCC	3 cut(s)	191, 878, 1566
AsuC2I	CCSGG	4 cut(s)	1445, 1446, 1570, 1673
AsuHPI	GGTGA	4 cut(s)	524, 807, 1568, 1784
AvaI	CYCGRG	2 cut(s)	1345, 1444
AvaII	GGWCC	3 cut(s)	191, 878, 1566
BaeGI	GKGCMC	1 cut(s)	1818
BamHI	GGATCC	2 cut(s)	457, 1440
BanI	GGYRCC	1 cut(s)	313
BbsI	GAAGAC	1 cut(s)	515
Bbv12I	GWGCWC	3 cut(s)	625, 1684, 1726
BbvCI	CCTCAGC	2 cut(s)	1254, 1397
BbvI	GCAGC	7 cut(s)	32, 562, 694, 952, 1562, 1895, 1912
BccI	CCATC	7 cut(s)	182, 658, 1086, 1216, 1268, 1606, 1712
BciT130I	CCWGG	7 cut(s)	303, 451, 820, 903, 1172, 1414, 1524
BciVI	GTATCC	2 cut(s)	929, 1579
BcnI	CCSGG	4 cut(s)	1445, 1446, 1570, 1673
BcoDI	GTCTC	4 cut(s)	635, 749, 1208, 1283
BfaI	CTAG	6 cut(s)	465, 579, 618, 627, 990, 1002
BfmI	CTRYAG	1 cut(s)	784
BfoI	RGCGCY	1 cut(s)	317
BfuI	GTATCC	2 cut(s)	929, 1579
BisI	GCNGC	8 cut(s)	21, 576, 683, 966, 1551, 1884, 1919, 1926
BlsI	GCNGC	8 cut(s)	22, 577, 684, 967, 1552, 1885, 1920, 1927
Bme18I	GGWCC	3 cut(s)	191, 878, 1566
BmeT110I	CYCGRG	2 cut(s)	1345, 1444
BmgT120I	GGNCC	3 cut(s)	191, 878, 1566
BmiI	GGNNCC	7 cut(s)	193, 315, 459, 751, 889, 1442, 1568
BmrI	ACTGGG	3 cut(s)	629, 1147, 1299
BmsI	GCATC	6 cut(s)	173, 193, 215, 718, 1541, 1815
BmuI	ACTGGG	3 cut(s)	629, 1147, 1299
BoxI	GACNNNNGTC	1 cut(s)	997
BpiI	GAAGAC	1 cut(s)	515
BplI	GAGNNNNNCTC	4 cut(s)	1618, 1650, 1771, 1803
BpmI	CTGGAG	4 cut(s)	324, 768, 924, 1193
Bpu10I	CCTNAGC	3 cut(s)	147, 1254, 1397
BpuEI	CTTGAG	2 cut(s)	19, 1710
BpuMI	CCSGG	4 cut(s)	1445, 1446, 1570, 1673
BsaBI	GATNNNNATC	2 cut(s)	1439, 1531
BsaHI	GRCGYC	1 cut(s)	314
BsaI	GGTCTC	1 cut(s)	1283
BsaJI	CCNNGG	7 cut(s)	118, 332, 430, 449, 818, 923, 1444
BsaXI	ACNNNNNCTCC	4 cut(s)	445, 475, 897, 927
Bsc4I	CCNNNNNNNGG	2 cut(s)	1419, 1485
Bse1I	ACTGG	5 cut(s)	550, 635, 751, 1142, 1305
Bse3DI	GCAATG	1 cut(s)	738
Bse8I	GATNNNNATC	2 cut(s)	1439, 1531
BseBI	CCWGG	7 cut(s)	303, 451, 820, 903, 1172, 1414, 1524
BseDI	CCNNGG	7 cut(s)	118, 332, 430, 449, 818, 923, 1444
BseGI	GGATG	7 cut(s)	157, 193, 607, 1227, 1723, 1739, 1765
BseJI	GATNNNNATC	2 cut(s)	1439, 1531
BseLI	CCNNNNNNNGG	2 cut(s)	1419, 1485
BseMI	GCAATG	1 cut(s)	738
BseMII	CTCAG	5 cut(s)	138, 1136, 1245, 1411, 1802
BseNI	ACTGG	5 cut(s)	550, 635, 751, 1142, 1305
BseRI	GAGGAG	1 cut(s)	1642
BseSI	GKGCMC	1 cut(s)	1818
BseXI	GCAGC	7 cut(s)	32, 562, 694, 952, 1562, 1895, 1912
BseYI	CCCAGC	1 cut(s)	160
BsgI	GTGCAG	1 cut(s)	39
BshFI	GGCC	3 cut(s)	426, 1353, 1633
BshNI	GGYRCC	1 cut(s)	313
BsiHKAI	GWGCWC	3 cut(s)	625, 1684, 1726
BsiHKCI	CYCGRG	2 cut(s)	1345, 1444
BsiSI	CCGG	3 cut(s)	1445, 1570, 1672
BslFI	GGGAC	3 cut(s)	177, 305, 1552
BslI	CCNNNNNNNGG	2 cut(s)	1419, 1485
BsmAI	GTCTC	4 cut(s)	635, 749, 1208, 1283
BsmFI	GGGAC	3 cut(s)	177, 305, 1552
BsnI	GGCC	3 cut(s)	426, 1353, 1633
Bso31I	GGTCTC	1 cut(s)	1283
BsoBI	CYCGRG	2 cut(s)	1345, 1444
Bsp1286I	GDGCHC	4 cut(s)	625, 1684, 1726, 1818
Bsp143I	GATC	7 cut(s)	343, 457, 1066, 1225, 1440, 1491, 1675
Bsp19I	CCATGG	1 cut(s)	430
BspACI	CCGC	3 cut(s)	1328, 1792, 1919
BspANI	GGCC	3 cut(s)	426, 1353, 1633
BspCNI	CTCAG	5 cut(s)	137, 1135, 1246, 1410, 1801
BspHI	TCATGA	1 cut(s)	1684
BspLI	GGNNCC	7 cut(s)	193, 315, 459, 751, 889, 1442, 1568
BspPI	GGATC	7 cut(s)	338, 452, 465, 1233, 1435, 1448, 1683
BspQI	GCTCTTC	1 cut(s)	1160
BspT107I	GGYRCC	1 cut(s)	313
BspTNI	GGTCTC	1 cut(s)	1283
BsrDI	GCAATG	1 cut(s)	738
BsrI	ACTGG	5 cut(s)	550, 635, 751, 1142, 1305
BssECI	CCNNGG	7 cut(s)	118, 332, 430, 449, 818, 923, 1444
BssMI	GATC	7 cut(s)	343, 457, 1066, 1225, 1440, 1491, 1675
BssNAI	GTATAC	1 cut(s)	1618
BssNI	GRCGYC	1 cut(s)	314
BssT1I	CCWWGG	4 cut(s)	118, 332, 430, 923
Bst1107I	GTATAC	1 cut(s)	1618
Bst2UI	CCWGG	7 cut(s)	303, 451, 820, 903, 1172, 1414, 1524
Bst6I	CTCTTC	3 cut(s)	243, 1152, 1160
BstACI	GRCGYC	1 cut(s)	314
BstAPI	GCANNNNNTGC	1 cut(s)	848
BstC8I	GCNNGC	2 cut(s)	970, 1923
BstDEI	CTNAG	7 cut(s)	124, 147, 670, 1122, 1254, 1397, 1788
BstDSI	CCRYGG	1 cut(s)	430
BstEII	GGTNACC	2 cut(s)	530, 1310
BstF5I	GGATG	7 cut(s)	157, 193, 607, 1227, 1723, 1739, 1765
BstH2I	RGCGCY	1 cut(s)	317
BstHHI	GCGC	1 cut(s)	316
BstKTI	GATC	7 cut(s)	346, 460, 1069, 1228, 1443, 1494, 1678
BstMAI	GTCTC	4 cut(s)	635, 749, 1208, 1283
BstMBI	GATC	7 cut(s)	343, 457, 1066, 1225, 1440, 1491, 1675
BstMWI	GCNNNNNNNGC	3 cut(s)	848, 1639, 1706
BstNI	CCWGG	7 cut(s)	303, 451, 820, 903, 1172, 1414, 1524
BstNSI	RCATGY	3 cut(s)	1334, 1704, 1822
BstPAI	GACNNNNGTC	1 cut(s)	997
BstPI	GGTNACC	2 cut(s)	530, 1310
BstSFI	CTRYAG	1 cut(s)	784
BstSLI	GKGCMC	1 cut(s)	1818
BstV1I	GCAGC	7 cut(s)	32, 562, 694, 952, 1562, 1895, 1912
BstV2I	GAAGAC	1 cut(s)	515
BstX2I	RGATCY	4 cut(s)	343, 457, 1225, 1440
BstXI	CCANNNNNNTGG	2 cut(s)	1137, 1349
BstYI	RGATCY	4 cut(s)	343, 457, 1225, 1440
BstZ17I	GTATAC	1 cut(s)	1618
BsuI	GTATCC	2 cut(s)	929, 1579
BsuRI	GGCC	3 cut(s)	426, 1353, 1633
BtgI	CCRYGG	1 cut(s)	430
BtsCI	GGATG	7 cut(s)	157, 193, 607, 1227, 1723, 1739, 1765
BtsI	GCAGTG	1 cut(s)	960
BtsIMutI	CAGTG	2 cut(s)	960, 1312
Cac8I	GCNNGC	2 cut(s)	970, 1923
CaiI	CAGNNNCTG	3 cut(s)	374, 1553, 1886
CciI	TCATGA	1 cut(s)	1684
CfoI	GCGC	1 cut(s)	316
Cfr13I	GGNCC	3 cut(s)	191, 878, 1566
Cfr9I	CCCGGG	1 cut(s)	1444
CsiI	ACCWGGT	1 cut(s)	1412
Csp6I	GTAC	5 cut(s)	231, 547, 1033, 1098, 1260
CspCI	CAANNNNNGTGG	2 cut(s)	471, 506
CviAII	CATG	9 cut(s)	431, 1271, 1331, 1621, 1685, 1701, 1714, 1819, 1944
CviQI	GTAC	5 cut(s)	231, 547, 1033, 1098, 1260
DdeI	CTNAG	7 cut(s)	124, 147, 670, 1122, 1254, 1397, 1788
DinI	GGCGCC	1 cut(s)	315
DpnI	GATC	7 cut(s)	345, 459, 1068, 1227, 1442, 1493, 1677
DpnII	GATC	7 cut(s)	343, 457, 1066, 1225, 1440, 1491, 1675
DraI	TTTAAA	1 cut(s)	1045
Eam1104I	CTCTTC	3 cut(s)	243, 1152, 1160
EarI	CTCTTC	3 cut(s)	243, 1152, 1160
Eco130I	CCWWGG	4 cut(s)	118, 332, 430, 923
Eco147I	AGGCCT	1 cut(s)	1633
Eco31I	GGTCTC	1 cut(s)	1283
Eco47I	GGWCC	3 cut(s)	191, 878, 1566
Eco88I	CYCGRG	2 cut(s)	1345, 1444
Eco91I	GGTNACC	2 cut(s)	530, 1310
EcoO109I	RGGNCCY	1 cut(s)	1566
EcoO65I	GGTNACC	2 cut(s)	530, 1310
EcoRI	GAATTC	1 cut(s)	1149
EcoRII	CCWGG	7 cut(s)	301, 449, 818, 901, 1170, 1412, 1522
EcoT14I	CCWWGG	4 cut(s)	118, 332, 430, 923
EcoT22I	ATGCAT	2 cut(s)	208, 1534
EgeI	GGCGCC	1 cut(s)	315
EheI	GGCGCC	1 cut(s)	315
ErhI	CCWWGG	4 cut(s)	118, 332, 430, 923
FaeI	CATG	9 cut(s)	434, 1274, 1334, 1624, 1688, 1704, 1717, 1822, 1947
FalI	AAGNNNNNCTT	2 cut(s)	1496, 1528
FaqI	GGGAC	3 cut(s)	177, 305, 1552
FatI	CATG	9 cut(s)	430, 1270, 1330, 1620, 1684, 1700, 1713, 1818, 1943
FblI	GTMKAC	1 cut(s)	1617
Fnu4HI	GCNGC	8 cut(s)	21, 576, 683, 966, 1551, 1884, 1919, 1926
FokI	GGATG	7 cut(s)	164, 200, 594, 1234, 1730, 1746, 1772
Fsp4HI	GCNGC	8 cut(s)	21, 576, 683, 966, 1551, 1884, 1919, 1926
FspBI	CTAG	6 cut(s)	465, 579, 618, 627, 990, 1002
GlaI	GCGC	1 cut(s)	315
GluI	GCNGC	8 cut(s)	21, 576, 683, 966, 1551, 1884, 1919, 1926
GsaI	CCCAGC	1 cut(s)	164
GsuI	CTGGAG	4 cut(s)	324, 768, 924, 1193
HaeII	RGCGCY	1 cut(s)	317
HaeIII	GGCC	3 cut(s)	426, 1353, 1633
HapII	CCGG	3 cut(s)	1445, 1570, 1672
HhaI	GCGC	1 cut(s)	316
Hin1I	GRCGYC	1 cut(s)	314
Hin1II	CATG	9 cut(s)	434, 1274, 1334, 1624, 1688, 1704, 1717, 1822, 1947
Hin6I	GCGC	1 cut(s)	314
HinP1I	GCGC	1 cut(s)	314
HindIII	AAGCTT	1 cut(s)	502
HinfI	GANTC	4 cut(s)	830, 1120, 1206, 1802
HpaII	CCGG	3 cut(s)	1445, 1570, 1672
HphI	GGTGA	4 cut(s)	524, 807, 1568, 1784
Hpy166II	GTNNAC	5 cut(s)	1100, 1336, 1455, 1618, 1842
Hpy188I	TCNGA	6 cut(s)	202, 343, 415, 829, 1075, 1638
Hpy188III	TCNNGA	6 cut(s)	71, 375, 1154, 1495, 1685, 1757
Hpy8I	GTNNAC	5 cut(s)	1100, 1336, 1455, 1618, 1842
Hpy99I	CGWCG	1 cut(s)	1109
HpyAV	CCTTC	5 cut(s)	274, 1309, 1364, 1412, 1600
HpyCH4V	TGCA	9 cut(s)	20, 206, 271, 682, 731, 968, 1027, 1532, 1928
HpyF10VI	GCNNNNNNNGC	3 cut(s)	848, 1639, 1706
HpyF3I	CTNAG	7 cut(s)	124, 147, 670, 1122, 1254, 1397, 1788
Hsp92I	GRCGYC	1 cut(s)	314
Hsp92II	CATG	9 cut(s)	434, 1274, 1334, 1624, 1688, 1704, 1717, 1822, 1947
HspAI	GCGC	1 cut(s)	314
KasI	GGCGCC	1 cut(s)	313
Kzo9I	GATC	7 cut(s)	343, 457, 1066, 1225, 1440, 1491, 1675
LguI	GCTCTTC	1 cut(s)	1160
LmnI	GCTCC	5 cut(s)	628, 641, 674, 749, 1250
Lsp1109I	GCAGC	7 cut(s)	32, 562, 694, 952, 1562, 1895, 1912
LweI	GCATC	6 cut(s)	173, 193, 215, 718, 1541, 1815
MabI	ACCWGGT	1 cut(s)	1412
MaeI	CTAG	6 cut(s)	465, 579, 618, 627, 990, 1002
MaeIII	GTNAC	5 cut(s)	530, 813, 958, 1310, 1772
MalI	GATC	7 cut(s)	345, 459, 1068, 1227, 1442, 1493, 1677
MboI	GATC	7 cut(s)	343, 457, 1066, 1225, 1440, 1491, 1675
MboII	GAAGA	7 cut(s)	93, 260, 520, 696, 1076, 1169, 1177
MfeI	CAATTG	2 cut(s)	882, 995
MflI	RGATCY	4 cut(s)	343, 457, 1225, 1440
MhlI	GDGCHC	4 cut(s)	625, 1684, 1726, 1818
Mly113I	GGCGCC	1 cut(s)	314
MlyI	GAGTC	2 cut(s)	1114, 1215
MmeI	TCCRAC	2 cut(s)	619, 1228
Mph1103I	ATGCAT	2 cut(s)	208, 1534
MroXI	GAANNNNTTC	1 cut(s)	382
MseI	TTAA	6 cut(s)	477, 930, 1044, 1515, 1604, 1797
MslI	CAYNNNNRTG	6 cut(s)	435, 537, 1347, 1694, 1823, 1851
MspA1I	CMGCKG	3 cut(s)	1553, 1792, 1886
MspI	CCGG	3 cut(s)	1445, 1570, 1672
MunI	CAATTG	2 cut(s)	882, 995
MvaI	CCWGG	7 cut(s)	303, 451, 820, 903, 1172, 1414, 1524
MwoI	GCNNNNNNNGC	3 cut(s)	848, 1639, 1706
NarI	GGCGCC	1 cut(s)	314
NciI	CCSGG	4 cut(s)	1445, 1446, 1570, 1673
NcoI	CCATGG	1 cut(s)	430
NdeII	GATC	7 cut(s)	343, 457, 1066, 1225, 1440, 1491, 1675
NlaIII	CATG	9 cut(s)	434, 1274, 1334, 1624, 1688, 1704, 1717, 1822, 1947
NlaIV	GGNNCC	7 cut(s)	193, 315, 459, 751, 889, 1442, 1568
NmeAIII	GCCGAG	1 cut(s)	778
NmuCI	GTSAC	4 cut(s)	530, 813, 958, 1772
NsiI	ATGCAT	2 cut(s)	208, 1534
NspI	RCATGY	3 cut(s)	1334, 1704, 1822
OliI	CACNNNNGTG	1 cut(s)	1347
PagI	TCATGA	1 cut(s)	1684
PceI	AGGCCT	1 cut(s)	1633
PciSI	GCTCTTC	1 cut(s)	1160
PdmI	GAANNNNTTC	1 cut(s)	382
PfeI	GAWTC	2 cut(s)	830, 1802
PfoI	TCCNGGA	3 cut(s)	301, 901, 1671
PkrI	GCNGC	8 cut(s)	22, 577, 684, 967, 1552, 1885, 1920, 1927
PleI	GAGTC	2 cut(s)	1114, 1214
PluTI	GGCGCC	1 cut(s)	317
PpsI	GAGTC	2 cut(s)	1114, 1214
PpuMI	RGGWCCY	1 cut(s)	1566
PshAI	GACNNNNGTC	1 cut(s)	997
Psp5II	RGGWCCY	1 cut(s)	1566
Psp6I	CCWGG	7 cut(s)	301, 449, 818, 901, 1170, 1412, 1522
PspEI	GGTNACC	2 cut(s)	530, 1310
PspFI	CCCAGC	1 cut(s)	160
PspGI	CCWGG	7 cut(s)	301, 449, 818, 901, 1170, 1412, 1522
PspN4I	GGNNCC	7 cut(s)	193, 315, 459, 751, 889, 1442, 1568
PspPI	GGNCC	3 cut(s)	191, 878, 1566
PspPPI	RGGWCCY	1 cut(s)	1566
PsrI	GAACNNNNNNTAC	2 cut(s)	349, 381
PstNI	CAGNNNCTG	3 cut(s)	374, 1553, 1886
PsuI	RGATCY	4 cut(s)	343, 457, 1225, 1440
PvuII	CAGCTG	2 cut(s)	1553, 1886
RsaI	GTAC	5 cut(s)	232, 548, 1034, 1099, 1261
RsaNI	GTAC	5 cut(s)	231, 547, 1033, 1098, 1260
RseI	CAYNNNNRTG	6 cut(s)	435, 537, 1347, 1694, 1823, 1851
SapI	GCTCTTC	1 cut(s)	1160
SaqAI	TTAA	6 cut(s)	477, 930, 1044, 1515, 1604, 1797
SatI	GCNGC	8 cut(s)	21, 576, 683, 966, 1551, 1884, 1919, 1926
Sau3AI	GATC	7 cut(s)	343, 457, 1066, 1225, 1440, 1491, 1675
Sau96I	GGNCC	3 cut(s)	191, 878, 1566
SchI	GAGTC	2 cut(s)	1114, 1215
SduI	GDGCHC	4 cut(s)	625, 1684, 1726, 1818
SexAI	ACCWGGT	1 cut(s)	1412
SfaNI	GCATC	6 cut(s)	173, 193, 215, 718, 1541, 1815
SfcI	CTRYAG	1 cut(s)	784
SfoI	GGCGCC	1 cut(s)	315
SinI	GGWCC	3 cut(s)	191, 878, 1566
SmaI	CCCGGG	1 cut(s)	1446
SmiMI	CAYNNNNRTG	6 cut(s)	435, 537, 1347, 1694, 1823, 1851
SmlI	CTYRAG	2 cut(s)	34, 1725
SmoI	CTYRAG	2 cut(s)	34, 1725
SseBI	AGGCCT	1 cut(s)	1633
SsiI	CCGC	3 cut(s)	1328, 1792, 1919
SspDI	GGCGCC	1 cut(s)	313
SspMI	CTAG	6 cut(s)	465, 579, 618, 627, 990, 1002
StuI	AGGCCT	1 cut(s)	1633
StyI	CCWWGG	4 cut(s)	118, 332, 430, 923
TaqI	TCGA	6 cut(s)	282, 716, 741, 1104, 1357, 1427
TaqII	GACCGA	1 cut(s)	518
TatI	WGTACW	2 cut(s)	230, 1032
TauI	GCSGC	1 cut(s)	1921
TfiI	GAWTC	2 cut(s)	830, 1802
Tru1I	TTAA	6 cut(s)	477, 930, 1044, 1515, 1604, 1797
Tru9I	TTAA	6 cut(s)	477, 930, 1044, 1515, 1604, 1797
TscAI	CASTG	2 cut(s)	967, 1312
TseFI	GTSAC	4 cut(s)	530, 813, 958, 1772
TseI	GCWGC	7 cut(s)	20, 575, 682, 965, 1550, 1883, 1925
Tsp45I	GTSAC	4 cut(s)	530, 813, 958, 1772
TspDTI	ATGAA	5 cut(s)	422, 749, 1032, 1553, 1932
TspGWI	ACGGA	1 cut(s)	470
TspMI	CCCGGG	1 cut(s)	1444
TspRI	CASTG	2 cut(s)	967, 1312
VpaK11BI	GGWCC	3 cut(s)	191, 878, 1566
XapI	RAATTY	2 cut(s)	1149, 1369
XceI	RCATGY	3 cut(s)	1334, 1704, 1822
XcmI	CCANNNNNNNNNTGG	2 cut(s)	92, 1134
XmaI	CCCGGG	1 cut(s)	1444
XmiI	GTMKAC	1 cut(s)	1617
XmnI	GAANNNNTTC	1 cut(s)	382
XspI	CTAG	6 cut(s)	465, 579, 618, 627, 990, 1002
Zsp2I	ATGCAT	2 cut(s)	208, 1534

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.