Rorug05G0142800

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004980
Physical Location & Seq
Forward (+)
13117365 .. 13118225
861 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0142800.1

Sequence Viewer

Length: 690 bp
ATGGCTACTGCTAGCGTTGTACCCAGTTCTAATGGCTCTGCTCGGGTGTCTTTAGGCCTGGCAGGGACGGCGGCTTTGCAGGATGACAATAACTACATGGTGGGTCGTCTCCTTGGACCTAAAGCTAGGTTTCCGGGATTCAAAAATACAATTCCTTCTATTTGGCGCATCAGATCTGGTCTTACAATCCAAGATGTCGGTGACCGTTTCTTGTTCCAATTTGTTCGAGAAGCGGATCAGAATCGTGCTCTCCATGGTGGCCCTTGGTTCTATAGGAACACCATGTTCTTGTTGGGTGAATATGACTGGATCGGTTCTGTGGAGGCAGTCCCTTTGTGGAGTGGGGAGACATGGGTGGCAGTAAGAGGTTTGCCGGTAGCCCTTCGGAACAAGGTCGCCCTAACTCTCATCGGATTAGTGACTGGAGATTGTGGACTCTTTGAACATGATGTGTTGGGATGTGATGGTTTTCTCATGAAGGAAAAGGAAGAGCTGATGCAGAAGCTGCTGGAGGTGGTGATGGCAGGACTGTCACTGGAGGGTCAGGGGCGGAACTTGGGTTCTAGTTCTATTTCGTGGGAGCCCTTAGTGCAGATGGAAGGGAATGATGTAGGGATGGAGATGATGGTGGTTCCTGAACGTGTACAACGCAGGTGGCTACTGATATGCAGAATGGGCTGCATGGGCTAG

Protein Analysis

229

Amino Acids

25.27

Weight (kDa)

5.77

Isoelectric Point (pI)

34.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 31 - 98 8.7e-09 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 642
Acc36I ACCTGC 1 cut(s) 642
AciI CCGC 3 cut(s) 71, 233, 550
AclWI GGATC 2 cut(s) 243, 317
AfaI GTAC 2 cut(s) 21, 645
AflIII ACRYGT 1 cut(s) 640
AgsI TTSAA 2 cut(s) 142, 443
AjnI CCWGG 1 cut(s) 57
AloI GAACNNNNNNTCC 2 cut(s) 544, 576
AluBI AGCT 3 cut(s) 125, 493, 505
AluI AGCT 3 cut(s) 125, 493, 505
Alw21I GWGCWC 1 cut(s) 250
Alw26I GTCTC 2 cut(s) 113, 341
AlwI GGATC 2 cut(s) 243, 317
AlwNI CAGNNNCTG 1 cut(s) 505
Ama87I CYCGRG 1 cut(s) 42
AoxI GGCC 2 cut(s) 55, 259
ApeKI GCWGC 2 cut(s) 505, 678
ArsI GACNNNNNNTTYG 2 cut(s) 58, 90
AspLEI GCGC 1 cut(s) 168
AspS9I GGNCC 2 cut(s) 116, 260
AsuC2I CCSGG 1 cut(s) 135
AsuHPI GGTGA 3 cut(s) 212, 308, 529
AsuNHI GCTAGC 1 cut(s) 11
AvaI CYCGRG 1 cut(s) 42
AvaII GGWCC 1 cut(s) 116
BanII GRGCYC 1 cut(s) 585
Bbv12I GWGCWC 1 cut(s) 250
BbvI GCAGC 2 cut(s) 492, 665
BccI CCATC 5 cut(s) 458, 514, 589, 610, 619
BceAI ACGGC 1 cut(s) 84
BciT130I CCWGG 1 cut(s) 59
BcnI CCSGG 1 cut(s) 135
BcoDI GTCTC 2 cut(s) 113, 341
BfaI CTAG 4 cut(s) 12, 126, 564, 688
BfmI CTRYAG 1 cut(s) 271
BfuAI ACCTGC 1 cut(s) 642
BglII AGATCT 1 cut(s) 173
BisI GCNGC 3 cut(s) 72, 506, 679
BlsI GCNGC 3 cut(s) 73, 507, 680
Bme1390I CCNGG 2 cut(s) 59, 135
Bme18I GGWCC 1 cut(s) 116
BmeT110I CYCGRG 1 cut(s) 42
BmgT120I GGNCC 2 cut(s) 116, 260
BmiI GGNNCC 2 cut(s) 582, 633
BmrFI CCNGG 2 cut(s) 59, 135
BmrI ACTGGG 1 cut(s) 18
BmsI GCATC 2 cut(s) 177, 486
BmtI GCTAGC 1 cut(s) 15
BmuI ACTGGG 1 cut(s) 18
BpmI CTGGAG 3 cut(s) 444, 530, 557
BpuMI CCSGG 1 cut(s) 135
BsaBI GATNNNNATC 1 cut(s) 240
BsaJI CCNNGG 3 cut(s) 112, 253, 263
Bse118I RCCGGY 1 cut(s) 373
Bse1I ACTGG 4 cut(s) 24, 311, 427, 540
Bse8I GATNNNNATC 1 cut(s) 240
BseBI CCWGG 1 cut(s) 59
BseDI CCNNGG 3 cut(s) 112, 253, 263
BseGI GGATG 3 cut(s) 88, 464, 621
BseJI GATNNNNATC 1 cut(s) 240
BseNI ACTGG 4 cut(s) 24, 311, 427, 540
BseXI GCAGC 2 cut(s) 492, 665
BsgI GTGCAG 1 cut(s) 611
BshFI GGCC 2 cut(s) 57, 261
BsiHKAI GWGCWC 1 cut(s) 250
BsiHKCI CYCGRG 1 cut(s) 42
BsiSI CCGG 2 cut(s) 134, 374
BslFI GGGAC 2 cut(s) 79, 314
BsmAI GTCTC 2 cut(s) 113, 341
BsmBI CGTCTC 1 cut(s) 113
BsmFI GGGAC 2 cut(s) 79, 314
BsnI GGCC 2 cut(s) 57, 261
BsoBI CYCGRG 1 cut(s) 42
Bsp1286I GDGCHC 2 cut(s) 250, 585
Bsp1407I TGTACA 1 cut(s) 643
Bsp143I GATC 3 cut(s) 173, 235, 309
Bsp19I CCATGG 1 cut(s) 253
BspACI CCGC 3 cut(s) 71, 233, 550
BspANI GGCC 2 cut(s) 57, 261
BspHI TCATGA 1 cut(s) 474
BspLI GGNNCC 2 cut(s) 582, 633
BspMI ACCTGC 1 cut(s) 642
BspOI GCTAGC 1 cut(s) 15
BspPI GGATC 2 cut(s) 243, 317
BspQI GCTCTTC 1 cut(s) 483
BsrFI RCCGGY 1 cut(s) 373
BsrGI TGTACA 1 cut(s) 643
BsrI ACTGG 4 cut(s) 24, 311, 427, 540
BssAI RCCGGY 1 cut(s) 373
BssECI CCNNGG 3 cut(s) 112, 253, 263
BssMI GATC 3 cut(s) 173, 235, 309
BssT1I CCWWGG 3 cut(s) 112, 253, 263
Bst2UI CCWGG 1 cut(s) 59
Bst4CI ACNGT 2 cut(s) 206, 531
Bst6I CTCTTC 1 cut(s) 483
BstAPI GCANNNNNTGC 1 cut(s) 505
BstAUI TGTACA 1 cut(s) 643
BstC8I GCNNGC 1 cut(s) 13
BstDEI CTNAG 1 cut(s) 586
BstDSI CCRYGG 1 cut(s) 253
BstEII GGTNACC 1 cut(s) 200
BstF5I GGATG 3 cut(s) 88, 464, 621
BstHHI GCGC 1 cut(s) 168
BstKTI GATC 3 cut(s) 176, 238, 312
BstMAI GTCTC 2 cut(s) 113, 341
BstMBI GATC 3 cut(s) 173, 235, 309
BstMWI GCNNNNNNNGC 5 cut(s) 68, 505, 589, 675, 684
BstNI CCWGG 1 cut(s) 59
BstPI GGTNACC 1 cut(s) 200
BstSCI CCNGG 2 cut(s) 57, 133
BstSFI CTRYAG 1 cut(s) 271
BstV1I GCAGC 2 cut(s) 492, 665
BstX2I RGATCY 1 cut(s) 173
BstYI RGATCY 1 cut(s) 173
BsuRI GGCC 2 cut(s) 57, 261
BtgI CCRYGG 1 cut(s) 253
BtsCI GGATG 3 cut(s) 88, 464, 621
BtsIMutI CAGTG 1 cut(s) 533
BveI ACCTGC 1 cut(s) 642
Cac8I GCNNGC 1 cut(s) 13
CaiI CAGNNNCTG 1 cut(s) 505
CciI TCATGA 1 cut(s) 474
CfoI GCGC 1 cut(s) 168
Cfr10I RCCGGY 1 cut(s) 373
Cfr13I GGNCC 2 cut(s) 116, 260
Csp6I GTAC 2 cut(s) 20, 644
CspCI CAANNNNNGTGG 2 cut(s) 635, 670
CviAII CATG 7 cut(s) 97, 254, 283, 351, 446, 475, 682
CviQI GTAC 2 cut(s) 20, 644
DdeI CTNAG 1 cut(s) 586
DpnI GATC 3 cut(s) 175, 237, 311
DpnII GATC 3 cut(s) 173, 235, 309
Eam1104I CTCTTC 1 cut(s) 483
EarI CTCTTC 1 cut(s) 483
EciI GGCGGA 1 cut(s) 565
Eco130I CCWWGG 3 cut(s) 112, 253, 263
Eco147I AGGCCT 1 cut(s) 57
Eco24I GRGCYC 1 cut(s) 585
Eco47I GGWCC 1 cut(s) 116
Eco88I CYCGRG 1 cut(s) 42
Eco91I GGTNACC 1 cut(s) 200
EcoO65I GGTNACC 1 cut(s) 200
EcoRII CCWGG 1 cut(s) 57
EcoT14I CCWWGG 3 cut(s) 112, 253, 263
EcoT38I GRGCYC 1 cut(s) 585
ErhI CCWWGG 3 cut(s) 112, 253, 263
Esp3I CGTCTC 1 cut(s) 113
FaeI CATG 7 cut(s) 100, 257, 286, 354, 449, 478, 685
FaqI GGGAC 2 cut(s) 79, 314
FatI CATG 7 cut(s) 96, 253, 282, 350, 445, 474, 681
Fnu4HI GCNGC 3 cut(s) 72, 506, 679
FokI GGATG 3 cut(s) 95, 471, 628
FriOI GRGCYC 1 cut(s) 585
Fsp4HI GCNGC 3 cut(s) 72, 506, 679
FspBI CTAG 4 cut(s) 12, 126, 564, 688
GlaI GCGC 1 cut(s) 167
GluI GCNGC 3 cut(s) 72, 506, 679
GsuI CTGGAG 3 cut(s) 444, 530, 557
HaeIII GGCC 2 cut(s) 57, 261
HapII CCGG 2 cut(s) 134, 374
HhaI GCGC 1 cut(s) 168
Hin1II CATG 7 cut(s) 100, 257, 286, 354, 449, 478, 685
Hin6I GCGC 1 cut(s) 166
HinP1I GCGC 1 cut(s) 166
HinfI GANTC 3 cut(s) 138, 241, 435
HpaII CCGG 2 cut(s) 134, 374
HphI GGTGA 3 cut(s) 212, 308, 529
Hpy166II GTNNAC 2 cut(s) 434, 644
Hpy188I TCNGA 4 cut(s) 173, 240, 387, 413
Hpy188III TCNNGA 3 cut(s) 227, 475, 635
Hpy8I GTNNAC 2 cut(s) 434, 644
HpyAV CCTTC 4 cut(s) 165, 392, 472, 593
HpyCH4III ACNGT 2 cut(s) 206, 531
HpyCH4IV ACGT 1 cut(s) 640
HpyCH4V TGCA 5 cut(s) 79, 499, 592, 669, 681
HpyF10VI GCNNNNNNNGC 5 cut(s) 68, 505, 589, 675, 684
HpyF3I CTNAG 1 cut(s) 586
HpySE526I ACGT 1 cut(s) 640
Hsp92II CATG 7 cut(s) 100, 257, 286, 354, 449, 478, 685
HspAI GCGC 1 cut(s) 166
Kzo9I GATC 3 cut(s) 173, 235, 309
LguI GCTCTTC 1 cut(s) 483
LmnI GCTCC 1 cut(s) 580
Lsp1109I GCAGC 2 cut(s) 492, 665
LweI GCATC 2 cut(s) 177, 486
MaeI CTAG 4 cut(s) 12, 126, 564, 688
MaeII ACGT 1 cut(s) 640
MaeIII GTNAC 3 cut(s) 200, 418, 531
MalI GATC 3 cut(s) 175, 237, 311
MboI GATC 3 cut(s) 173, 235, 309
MboII GAAGA 1 cut(s) 500
MflI RGATCY 1 cut(s) 173
MhlI GDGCHC 2 cut(s) 250, 585
MluCI AATT 2 cut(s) 150, 218
MlyI GAGTC 1 cut(s) 429
MnlI CCTC 4 cut(s) 316, 359, 505, 532
MspI CCGG 2 cut(s) 134, 374
MspR9I CCNGG 2 cut(s) 59, 135
MvaI CCWGG 1 cut(s) 59
MwoI GCNNNNNNNGC 5 cut(s) 68, 505, 589, 675, 684
NciI CCSGG 1 cut(s) 135
NcoI CCATGG 1 cut(s) 253
NdeII GATC 3 cut(s) 173, 235, 309
NheI GCTAGC 1 cut(s) 11
NlaIII CATG 7 cut(s) 100, 257, 286, 354, 449, 478, 685
NlaIV GGNNCC 2 cut(s) 582, 633
NmuCI GTSAC 3 cut(s) 200, 418, 531
PagI TCATGA 1 cut(s) 474
PaqCI CACCTGC 1 cut(s) 642
PceI AGGCCT 1 cut(s) 57
PciSI GCTCTTC 1 cut(s) 483
PfeI GAWTC 2 cut(s) 138, 241
PfoI TCCNGGA 1 cut(s) 133
PkrI GCNGC 3 cut(s) 73, 507, 680
PleI GAGTC 1 cut(s) 429
PpsI GAGTC 1 cut(s) 429
Psp6I CCWGG 1 cut(s) 57
PspEI GGTNACC 1 cut(s) 200
PspGI CCWGG 1 cut(s) 57
PspN4I GGNNCC 2 cut(s) 582, 633
PspPI GGNCC 2 cut(s) 116, 260
PstNI CAGNNNCTG 1 cut(s) 505
PsuI RGATCY 1 cut(s) 173
RsaI GTAC 2 cut(s) 21, 645
RsaNI GTAC 2 cut(s) 20, 644
SapI GCTCTTC 1 cut(s) 483
SatI GCNGC 3 cut(s) 72, 506, 679
Sau3AI GATC 3 cut(s) 173, 235, 309
Sau96I GGNCC 2 cut(s) 116, 260
SchI GAGTC 1 cut(s) 429
ScrFI CCNGG 2 cut(s) 59, 135
SduI GDGCHC 2 cut(s) 250, 585
SfaNI GCATC 2 cut(s) 177, 486
SfcI CTRYAG 1 cut(s) 271
SinI GGWCC 1 cut(s) 116
Sse9I AATT 2 cut(s) 150, 218
SseBI AGGCCT 1 cut(s) 57
SsiI CCGC 3 cut(s) 71, 233, 550
SspMI CTAG 4 cut(s) 12, 126, 564, 688
StuI AGGCCT 1 cut(s) 57
StyD4I CCNGG 2 cut(s) 57, 133
StyI CCWWGG 3 cut(s) 112, 253, 263
TaaI ACNGT 2 cut(s) 206, 531
TaiI ACGT 1 cut(s) 643
TaqI TCGA 1 cut(s) 226
TasI AATT 2 cut(s) 150, 218
TatI WGTACW 1 cut(s) 643
TauI GCSGC 1 cut(s) 74
TfiI GAWTC 2 cut(s) 138, 241
TscAI CASTG 1 cut(s) 540
TseFI GTSAC 3 cut(s) 200, 418, 531
TseI GCWGC 2 cut(s) 505, 678
Tsp45I GTSAC 3 cut(s) 200, 418, 531
TspDTI ATGAA 1 cut(s) 491
TspRI CASTG 1 cut(s) 540
VpaK11BI GGWCC 1 cut(s) 116
XcmI CCANNNNNNNNNTGG 1 cut(s) 289
XspI CTAG 4 cut(s) 12, 126, 564, 688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.