Rh5AG233600

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0004980
Physical Location & Seq
Forward (+)
28294846 .. 28300050
5205 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG233600.1

Sequence Viewer

Length: 2148 bp
ATGGAGCCGCGGCTAAACTACGCACCCGAGAATTCTAATAACAAGCGAGCTCATTCCGGCATGGATACCGACGACGTTGTCGAGGTCGCCGCTCCGGTTTCTTGCTCTCGGAAATTGCGAAAGAACAAGGAGGTGAATTTGCAGGATGTGATTGAATTGGACAAAGATGATTCTGCTGCTGCAATGTTTATTTATGAGAAAATTGACAGAAGTATCAAAGGAAAGGCAGTGGAAAATTCTTCAGATGGTTACCATATTCATCAATCAAAGGAAGCTGTCCTCCATACTTCTGTTGGTTCTTCTGGTATGGAAACCCTTGGCTCAGTCAGTGGAATTGAAATTGCTAAGGATGTCGCCCCAGCATCAAATCACTTTATTGACTTGGATGGTCCCAATTCTGATGCATCTGCTGACGATGTTGATGACTGTACTGATACTTGTTTTGAAGAGTTTGATTATGCCTTCTTGCAATCCCATTTCGATAATGTGGGTATTCCTCCTGGAGTAGAGGCGCCTGTCCCTCATATTCCTTGGTTGTCAGATCCTCCTAAAAGTAATGTAAGTTCAGTTTCTGGAAGTAGTTCTGTTGATACAAGGTATCAAATGAAATCAGATTATGTTGGCCTCCATGGAAATGAGCTGTTTCCCTGGACAGATCCTCCTAGTTTCAACTTTAAGCCAACCGTTGTGGGTAGTTCAAGCTTGAAGACCCAAACAGACAGTATCGGTCACCCAAATGGACAGTACCAGTCATCTTGGAAACTTCCAAAAGCTGCTAGAAGCAGAAAGAAACAATCTGCTTCACATCCCCAAGTTAGTGCTCCTAGTCTCCCAGTTGGAGCAGAACCATCAAAATCTCAAAGGCTCTTAGGAGCTTTGCAGCGTAAAAAGAAGCTATTTTCTTCAAGTAGTTCGACTAATTATGATGCAATGAAGTTCGATACTGGAGCCGAGACATCTTTTTTTGGACATAATTTACCCTACAGTCCAATTTATCCTCATGGATTACAGTCACCCTGGTTGTCAGATTCTTTGTCAGCAATTAGTGCTAAACCAAATCATTCAAGTTTCTACGGTCCAATTGGTTCCATATACCCTCCTGGAGAGTTAGTAGGTATCCCTTGGGTTAAGGGTGTTTCCCAAACTCAACACAATGTCACTGCTGCAAGCCTTTCAACAACCCCTGCTAGACAATTGTCTAGCAAGGAAATAGATGAAATTATGCAGAAGTACAAGGGTTTTAAACAATTTGATACAGTTGAAGATCATTCAGACCATCACTATATACTCTCTGGTACACTCAATGGTAATTCAATGACTCAGCCACCAAAGAACTGGGCAAAGAGAATTCAGGAAGAGTGGAAGAGCCTGGAGAAAGATTTGCCTGACACAGTATTTGTTAGAGTCTATGAGACAAGGATGGATCTTTTGAGAGCTGTAATTGTTGGAGCTGAGGGTACTCCTTACCATGATGGTCTCTTTTTCTTTGATGTTTCCTTCCCCAGTGGTTACCCCAATGTGCCACCGCATGTCCACTACCACTCGGGTGGCCTTCGACTAAATCCAAATTTGTATGGTTGTGGAAAAGTATGCCTCAGCCTTCTCAACACCTGGTCTGGGAACTCAAAAGAGATGTGGATCCCGGGTGTGTCCACCATTCTACAAGTCTTAGTGTCCATACAAGGGCTGATCTTGAATACAAAGCCCTACTTTAATGAGCCTGGATATGCATCTATGAATGGTTCAGCAGCTGGTGAAACAAGGTCCCAGCAGTATAATGAGGATACCTTCCTCCTATCTTTAACAACGATGGTATACATGATGAGGAGGCCTCCAAAGCATTTTGAGGATTTTGTTGTGGGGCATTTCCGGGATCGTGCTCATGACATCCTGGTGGCATGTAAAGCATACATGGATGGTGCTCAAGTGGGATGTCTGGTCAAAGGTGGGGTTCAGGATGTTGATGAGGGTGACAAGAGTTGTTCTCAGCGGTTTAAGGATTCTTTAGCAGGGCACATGCCGATGCTTGTCAAAGAGTTTACACATATAGGTGTCAAGGATTGCGAGAAATATGTATCAGCAGCTGTAAATGGGAACGAGCAGATTGGTAGTATGCCGCAGGCTGCAACATCAATGGTTTCATGTTGA

Protein Analysis

715

Amino Acids

78.43

Weight (kDa)

5.95

Isoelectric Point (pI)

43.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 449 - 573 1.4e-21 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 77
AccB1I GGYRCC 1 cut(s) 511
AccBSI CCGCTC 1 cut(s) 92
AccI GTMKAC 1 cut(s) 1815
AccII CGCG 1 cut(s) 10
AciI CCGC 6 cut(s) 8, 10, 90, 1526, 1990, 2117
AclWI GGATC 6 cut(s) 536, 650, 1431, 1633, 1646, 1881
AcsI RAATTY 5 cut(s) 31, 136, 235, 1347, 1567
AcuI CTGAAG 1 cut(s) 225
AcyI GRCGYC 1 cut(s) 512
AfaI GTAC 5 cut(s) 430, 746, 1232, 1297, 1459
AfiI CCNNNNNNNGG 2 cut(s) 1617, 1683
AjnI CCWGG 8 cut(s) 499, 647, 1016, 1099, 1368, 1610, 1720, 1890
AleI CACNNNNGTG 1 cut(s) 1545
Alw21I GWGCWC 4 cut(s) 52, 823, 1882, 1924
Alw26I GTCTC 4 cut(s) 833, 947, 1406, 1481
AlwI GGATC 6 cut(s) 536, 650, 1431, 1633, 1646, 1881
AlwNI CAGNNNCTG 3 cut(s) 572, 1751, 2084
Ama87I CYCGRG 3 cut(s) 26, 1543, 1642
AoxI GGCC 3 cut(s) 622, 1549, 1829
ApeKI GCWGC 8 cut(s) 176, 179, 773, 880, 1163, 1748, 2081, 2123
ApoI RAATTY 5 cut(s) 31, 136, 235, 1347, 1567
ArsI GACNNNNNNTTYG 2 cut(s) 1379, 1411
Asp700I GAANNNNTTC 1 cut(s) 580
AspLEI GCGC 1 cut(s) 514
AspS9I GGNCC 3 cut(s) 389, 1076, 1764
AsuC2I CCSGG 3 cut(s) 1643, 1644, 1871
AsuHPI GGTGA 5 cut(s) 145, 722, 1005, 1766, 1982
AvaI CYCGRG 3 cut(s) 26, 1543, 1642
AvaII GGWCC 3 cut(s) 389, 1076, 1764
BaeGI GKGCMC 1 cut(s) 2016
BaeI ACNNNNGTAYC 2 cut(s) 196, 229
BamHI GGATCC 1 cut(s) 1638
BanI GGYRCC 1 cut(s) 511
BanII GRGCYC 1 cut(s) 52
BbsI GAAGAC 1 cut(s) 713
Bbv12I GWGCWC 4 cut(s) 52, 823, 1882, 1924
BbvCI CCTCAGC 2 cut(s) 1452, 1595
BbvI GCAGC 8 cut(s) 163, 166, 760, 892, 1150, 1760, 2093, 2110
BccI CCATC 8 cut(s) 239, 380, 856, 1284, 1414, 1466, 1804, 1910
BciT130I CCWGG 8 cut(s) 501, 649, 1018, 1101, 1370, 1612, 1722, 1892
BciVI GTATCC 3 cut(s) 58, 1127, 1777
BcnI CCSGG 3 cut(s) 1643, 1644, 1871
BcoDI GTCTC 4 cut(s) 833, 947, 1406, 1481
BfaI CTAG 5 cut(s) 663, 777, 825, 1188, 1200
BfmI CTRYAG 1 cut(s) 982
BfoI RGCGCY 1 cut(s) 515
BfuI GTATCC 3 cut(s) 58, 1127, 1777
Bme18I GGWCC 3 cut(s) 389, 1076, 1764
BmeT110I CYCGRG 3 cut(s) 26, 1543, 1642
BmgT120I GGNCC 3 cut(s) 389, 1076, 1764
BmiI GGNNCC 7 cut(s) 6, 391, 513, 949, 1087, 1640, 1766
BmrI ACTGGG 3 cut(s) 827, 1345, 1497
BmsI GCATC 6 cut(s) 371, 391, 413, 916, 1739, 2013
BmuI ACTGGG 3 cut(s) 827, 1345, 1497
BoxI GACNNNNGTC 1 cut(s) 1195
BpiI GAAGAC 1 cut(s) 713
BplI GAGNNNNNCTC 4 cut(s) 1816, 1848, 1969, 2001
BpmI CTGGAG 4 cut(s) 522, 966, 1122, 1391
Bpu10I CCTNAGC 3 cut(s) 345, 1452, 1595
BpuEI CTTGAG 1 cut(s) 1908
BpuMI CCSGG 3 cut(s) 1643, 1644, 1871
BsaBI GATNNNNATC 2 cut(s) 1637, 1729
BsaHI GRCGYC 1 cut(s) 512
BsaI GGTCTC 1 cut(s) 1481
BsaJI CCNNGG 8 cut(s) 8, 316, 530, 628, 647, 1016, 1121, 1642
BsaWI WCCGGW 1 cut(s) 94
BsaXI ACNNNNNCTCC 4 cut(s) 643, 673, 1095, 1125
Bsc4I CCNNNNNNNGG 2 cut(s) 1617, 1683
Bse1I ACTGG 5 cut(s) 748, 833, 949, 1340, 1503
Bse3DI GCAATG 2 cut(s) 189, 936
Bse8I GATNNNNATC 2 cut(s) 1637, 1729
BseBI CCWGG 8 cut(s) 501, 649, 1018, 1101, 1370, 1612, 1722, 1892
BseDI CCNNGG 8 cut(s) 8, 316, 530, 628, 647, 1016, 1121, 1642
BseGI GGATG 9 cut(s) 151, 355, 391, 805, 1425, 1887, 1921, 1937, 1963
BseJI GATNNNNATC 2 cut(s) 1637, 1729
BseLI CCNNNNNNNGG 2 cut(s) 1617, 1683
BseMI GCAATG 2 cut(s) 189, 936
BseMII CTCAG 5 cut(s) 336, 1334, 1443, 1609, 2000
BseNI ACTGG 5 cut(s) 748, 833, 949, 1340, 1503
BseRI GAGGAG 1 cut(s) 1840
BseSI GKGCMC 1 cut(s) 2016
BseXI GCAGC 8 cut(s) 163, 166, 760, 892, 1150, 1760, 2093, 2110
BseYI CCCAGC 2 cut(s) 358, 1767
Bsh1236I CGCG 1 cut(s) 10
BshFI GGCC 3 cut(s) 624, 1551, 1831
BshNI GGYRCC 1 cut(s) 511
BsiHKAI GWGCWC 4 cut(s) 52, 823, 1882, 1924
BsiHKCI CYCGRG 3 cut(s) 26, 1543, 1642
BsiSI CCGG 4 cut(s) 57, 95, 1643, 1870
BslFI GGGAC 3 cut(s) 375, 503, 1750
BslI CCNNNNNNNGG 2 cut(s) 1617, 1683
BsmAI GTCTC 4 cut(s) 833, 947, 1406, 1481
BsmFI GGGAC 3 cut(s) 375, 503, 1750
BsnI GGCC 3 cut(s) 624, 1551, 1831
Bso31I GGTCTC 1 cut(s) 1481
BsoBI CYCGRG 3 cut(s) 26, 1543, 1642
Bsp1286I GDGCHC 5 cut(s) 52, 823, 1882, 1924, 2016
Bsp143I GATC 7 cut(s) 541, 655, 1264, 1423, 1638, 1689, 1873
Bsp19I CCATGG 1 cut(s) 628
BspACI CCGC 6 cut(s) 8, 10, 90, 1526, 1990, 2117
BspANI GGCC 3 cut(s) 624, 1551, 1831
BspCNI CTCAG 5 cut(s) 335, 1333, 1444, 1608, 1999
BspFNI CGCG 1 cut(s) 10
BspHI TCATGA 1 cut(s) 1882
BspLI GGNNCC 7 cut(s) 6, 391, 513, 949, 1087, 1640, 1766
BspPI GGATC 6 cut(s) 536, 650, 1431, 1633, 1646, 1881
BspQI GCTCTTC 1 cut(s) 1358
BspT107I GGYRCC 1 cut(s) 511
BspTNI GGTCTC 1 cut(s) 1481
BsrBI CCGCTC 1 cut(s) 92
BsrDI GCAATG 2 cut(s) 189, 936
BsrI ACTGG 5 cut(s) 748, 833, 949, 1340, 1503
BssECI CCNNGG 8 cut(s) 8, 316, 530, 628, 647, 1016, 1121, 1642
BssMI GATC 7 cut(s) 541, 655, 1264, 1423, 1638, 1689, 1873
BssNAI GTATAC 1 cut(s) 1816
BssNI GRCGYC 1 cut(s) 512
BssT1I CCWWGG 4 cut(s) 316, 530, 628, 1121
Bst1107I GTATAC 1 cut(s) 1816
Bst2UI CCWGG 8 cut(s) 501, 649, 1018, 1101, 1370, 1612, 1722, 1892
Bst4CI ACNGT 9 cut(s) 428, 685, 722, 744, 986, 1011, 1076, 1258, 1393
Bst6I CTCTTC 3 cut(s) 441, 1350, 1358
BstACI GRCGYC 1 cut(s) 512
BstAPI GCANNNNNTGC 1 cut(s) 1046
BstC8I GCNNGC 3 cut(s) 48, 1168, 2121
BstDEI CTNAG 8 cut(s) 322, 345, 868, 1320, 1452, 1595, 1669, 1986
BstDSI CCRYGG 2 cut(s) 8, 628
BstEII GGTNACC 3 cut(s) 248, 728, 1508
BstF5I GGATG 9 cut(s) 151, 355, 391, 805, 1425, 1887, 1921, 1937, 1963
BstFNI CGCG 1 cut(s) 10
BstH2I RGCGCY 1 cut(s) 515
BstHHI GCGC 1 cut(s) 514
BstKTI GATC 7 cut(s) 544, 658, 1267, 1426, 1641, 1692, 1876
BstMAI GTCTC 4 cut(s) 833, 947, 1406, 1481
BstMBI GATC 7 cut(s) 541, 655, 1264, 1423, 1638, 1689, 1873
BstMWI GCNNNNNNNGC 3 cut(s) 1046, 1837, 1904
BstNI CCWGG 8 cut(s) 501, 649, 1018, 1101, 1370, 1612, 1722, 1892
BstNSI RCATGY 3 cut(s) 1532, 1902, 2020
BstPAI GACNNNNGTC 1 cut(s) 1195
BstPI GGTNACC 3 cut(s) 248, 728, 1508
BstSFI CTRYAG 1 cut(s) 982
BstSLI GKGCMC 1 cut(s) 2016
BstUI CGCG 1 cut(s) 10
BstV1I GCAGC 8 cut(s) 163, 166, 760, 892, 1150, 1760, 2093, 2110
BstV2I GAAGAC 1 cut(s) 713
BstX2I RGATCY 4 cut(s) 541, 655, 1423, 1638
BstXI CCANNNNNNTGG 2 cut(s) 1335, 1547
BstYI RGATCY 4 cut(s) 541, 655, 1423, 1638
BstZ17I GTATAC 1 cut(s) 1816
BsuI GTATCC 3 cut(s) 58, 1127, 1777
BsuRI GGCC 3 cut(s) 624, 1551, 1831
BtgI CCRYGG 2 cut(s) 8, 628
BtsCI GGATG 9 cut(s) 151, 355, 391, 805, 1425, 1887, 1921, 1937, 1963
BtsI GCAGTG 2 cut(s) 234, 1158
BtsIMutI CAGTG 4 cut(s) 234, 334, 1158, 1510
Cac8I GCNNGC 3 cut(s) 48, 1168, 2121
CaiI CAGNNNCTG 3 cut(s) 572, 1751, 2084
CciI TCATGA 1 cut(s) 1882
CfoI GCGC 1 cut(s) 514
Cfr13I GGNCC 3 cut(s) 389, 1076, 1764
Cfr42I CCGCGG 1 cut(s) 11
Cfr9I CCCGGG 1 cut(s) 1642
CsiI ACCWGGT 1 cut(s) 1610
Csp6I GTAC 5 cut(s) 429, 745, 1231, 1296, 1458
CspCI CAANNNNNGTGG 2 cut(s) 669, 704
CviQI GTAC 5 cut(s) 429, 745, 1231, 1296, 1458
DdeI CTNAG 8 cut(s) 322, 345, 868, 1320, 1452, 1595, 1669, 1986
DinI GGCGCC 1 cut(s) 513
DpnI GATC 7 cut(s) 543, 657, 1266, 1425, 1640, 1691, 1875
DpnII GATC 7 cut(s) 541, 655, 1264, 1423, 1638, 1689, 1873
DraI TTTAAA 1 cut(s) 1243
DrdI GACNNNNNNGTC 1 cut(s) 77
DseDI GACNNNNNNGTC 1 cut(s) 77
Eam1104I CTCTTC 3 cut(s) 441, 1350, 1358
EarI CTCTTC 3 cut(s) 441, 1350, 1358
Ecl136II GAGCTC 1 cut(s) 50
Eco130I CCWWGG 4 cut(s) 316, 530, 628, 1121
Eco147I AGGCCT 1 cut(s) 1831
Eco24I GRGCYC 1 cut(s) 52
Eco31I GGTCTC 1 cut(s) 1481
Eco47I GGWCC 3 cut(s) 389, 1076, 1764
Eco53kI GAGCTC 1 cut(s) 50
Eco57I CTGAAG 1 cut(s) 225
Eco88I CYCGRG 3 cut(s) 26, 1543, 1642
Eco91I GGTNACC 3 cut(s) 248, 728, 1508
EcoICRI GAGCTC 1 cut(s) 50
EcoO109I RGGNCCY 1 cut(s) 1764
EcoO65I GGTNACC 3 cut(s) 248, 728, 1508
EcoRI GAATTC 2 cut(s) 31, 1347
EcoRII CCWGG 8 cut(s) 499, 647, 1016, 1099, 1368, 1610, 1720, 1890
EcoT14I CCWWGG 4 cut(s) 316, 530, 628, 1121
EcoT22I ATGCAT 2 cut(s) 406, 1732
EcoT38I GRGCYC 1 cut(s) 52
EgeI GGCGCC 1 cut(s) 513
EheI GGCGCC 1 cut(s) 513
ErhI CCWWGG 4 cut(s) 316, 530, 628, 1121
FalI AAGNNNNNCTT 2 cut(s) 1694, 1726
FaqI GGGAC 3 cut(s) 375, 503, 1750
FblI GTMKAC 1 cut(s) 1815
FokI GGATG 9 cut(s) 158, 362, 398, 792, 1432, 1874, 1928, 1944, 1970
FriOI GRGCYC 1 cut(s) 52
FspBI CTAG 5 cut(s) 663, 777, 825, 1188, 1200
GlaI GCGC 1 cut(s) 513
GsaI CCCAGC 2 cut(s) 362, 1771
GsuI CTGGAG 4 cut(s) 522, 966, 1122, 1391
HaeII RGCGCY 1 cut(s) 515
HaeIII GGCC 3 cut(s) 624, 1551, 1831
HapII CCGG 4 cut(s) 57, 95, 1643, 1870
HhaI GCGC 1 cut(s) 514
Hin1I GRCGYC 1 cut(s) 512
Hin6I GCGC 1 cut(s) 512
HinP1I GCGC 1 cut(s) 512
HindIII AAGCTT 1 cut(s) 700
HinfI GANTC 5 cut(s) 170, 1028, 1318, 1404, 2000
HpaII CCGG 4 cut(s) 57, 95, 1643, 1870
HphI GGTGA 5 cut(s) 145, 722, 1005, 1766, 1982
Hpy166II GTNNAC 5 cut(s) 1298, 1534, 1653, 1816, 2040
Hpy188I TCNGA 7 cut(s) 111, 244, 400, 541, 613, 1027, 1273
Hpy188III TCNNGA 5 cut(s) 573, 1352, 1693, 1883, 1955
Hpy8I GTNNAC 5 cut(s) 1298, 1534, 1653, 1816, 2040
Hpy99I CGWCG 2 cut(s) 74, 77
HpyAV CCTTC 5 cut(s) 472, 1507, 1562, 1610, 1798
HpyCH4III ACNGT 9 cut(s) 428, 685, 722, 744, 986, 1011, 1076, 1258, 1393
HpyCH4IV ACGT 1 cut(s) 75
HpyF10VI GCNNNNNNNGC 3 cut(s) 1046, 1837, 1904
HpyF3I CTNAG 8 cut(s) 322, 345, 868, 1320, 1452, 1595, 1669, 1986
HpySE526I ACGT 1 cut(s) 75
Hsp92I GRCGYC 1 cut(s) 512
HspAI GCGC 1 cut(s) 512
KasI GGCGCC 1 cut(s) 511
KspI CCGCGG 1 cut(s) 11
Kzo9I GATC 7 cut(s) 541, 655, 1264, 1423, 1638, 1689, 1873
LguI GCTCTTC 1 cut(s) 1358
LmnI GCTCC 7 cut(s) 4, 97, 826, 839, 872, 947, 1448
Lsp1109I GCAGC 8 cut(s) 163, 166, 760, 892, 1150, 1760, 2093, 2110
LweI GCATC 6 cut(s) 371, 391, 413, 916, 1739, 2013
MabI ACCWGGT 1 cut(s) 1610
MaeI CTAG 5 cut(s) 663, 777, 825, 1188, 1200
MaeII ACGT 1 cut(s) 75
MaeIII GTNAC 6 cut(s) 248, 728, 1011, 1156, 1508, 1970
MalI GATC 7 cut(s) 543, 657, 1266, 1425, 1640, 1691, 1875
MbiI CCGCTC 1 cut(s) 92
MboI GATC 7 cut(s) 541, 655, 1264, 1423, 1638, 1689, 1873
MboII GAAGA 8 cut(s) 231, 291, 458, 718, 894, 1274, 1367, 1375
MfeI CAATTG 2 cut(s) 1080, 1193
MflI RGATCY 4 cut(s) 541, 655, 1423, 1638
MhlI GDGCHC 5 cut(s) 52, 823, 1882, 1924, 2016
Mly113I GGCGCC 1 cut(s) 512
MlyI GAGTC 2 cut(s) 1312, 1413
MmeI TCCRAC 2 cut(s) 817, 1426
Mph1103I ATGCAT 2 cut(s) 406, 1732
MroXI GAANNNNTTC 1 cut(s) 580
MseI TTAA 6 cut(s) 675, 1128, 1242, 1713, 1802, 1995
MslI CAYNNNNRTG 6 cut(s) 633, 735, 1545, 1892, 2021, 2049
MspA1I CMGCKG 4 cut(s) 10, 1751, 1990, 2084
MspI CCGG 4 cut(s) 57, 95, 1643, 1870
MunI CAATTG 2 cut(s) 1080, 1193
MvaI CCWGG 8 cut(s) 501, 649, 1018, 1101, 1370, 1612, 1722, 1892
MvnI CGCG 1 cut(s) 10
MwoI GCNNNNNNNGC 3 cut(s) 1046, 1837, 1904
NarI GGCGCC 1 cut(s) 512
NciI CCSGG 3 cut(s) 1643, 1644, 1871
NcoI CCATGG 1 cut(s) 628
NdeII GATC 7 cut(s) 541, 655, 1264, 1423, 1638, 1689, 1873
NlaIV GGNNCC 7 cut(s) 6, 391, 513, 949, 1087, 1640, 1766
NmeAIII GCCGAG 1 cut(s) 976
NmuCI GTSAC 4 cut(s) 728, 1011, 1156, 1970
NsiI ATGCAT 2 cut(s) 406, 1732
NspI RCATGY 3 cut(s) 1532, 1902, 2020
OliI CACNNNNGTG 1 cut(s) 1545
PagI TCATGA 1 cut(s) 1882
PceI AGGCCT 1 cut(s) 1831
PciSI GCTCTTC 1 cut(s) 1358
PcsI WCGNNNNNNNCGW 2 cut(s) 78, 115
PdmI GAANNNNTTC 1 cut(s) 580
PfeI GAWTC 3 cut(s) 170, 1028, 2000
PflFI GACNNNGTC 1 cut(s) 77
PfoI TCCNGGA 3 cut(s) 499, 1099, 1869
PleI GAGTC 2 cut(s) 1312, 1412
PluTI GGCGCC 1 cut(s) 515
PpsI GAGTC 2 cut(s) 1312, 1412
PpuMI RGGWCCY 1 cut(s) 1764
PshAI GACNNNNGTC 1 cut(s) 1195
Psp124BI GAGCTC 1 cut(s) 52
Psp5II RGGWCCY 1 cut(s) 1764
Psp6I CCWGG 8 cut(s) 499, 647, 1016, 1099, 1368, 1610, 1720, 1890
PspEI GGTNACC 3 cut(s) 248, 728, 1508
PspFI CCCAGC 2 cut(s) 358, 1767
PspGI CCWGG 8 cut(s) 499, 647, 1016, 1099, 1368, 1610, 1720, 1890
PspN4I GGNNCC 7 cut(s) 6, 391, 513, 949, 1087, 1640, 1766
PspPI GGNCC 3 cut(s) 389, 1076, 1764
PspPPI RGGWCCY 1 cut(s) 1764
PsrI GAACNNNNNNTAC 2 cut(s) 547, 579
PstNI CAGNNNCTG 3 cut(s) 572, 1751, 2084
PsuI RGATCY 4 cut(s) 541, 655, 1423, 1638
PsyI GACNNNGTC 1 cut(s) 77
PvuII CAGCTG 2 cut(s) 1751, 2084
RsaI GTAC 5 cut(s) 430, 746, 1232, 1297, 1459
RsaNI GTAC 5 cut(s) 429, 745, 1231, 1296, 1458
RseI CAYNNNNRTG 6 cut(s) 633, 735, 1545, 1892, 2021, 2049
SacI GAGCTC 1 cut(s) 52
SacII CCGCGG 1 cut(s) 11
SapI GCTCTTC 1 cut(s) 1358
SaqAI TTAA 6 cut(s) 675, 1128, 1242, 1713, 1802, 1995
Sau3AI GATC 7 cut(s) 541, 655, 1264, 1423, 1638, 1689, 1873
Sau96I GGNCC 3 cut(s) 389, 1076, 1764
SchI GAGTC 2 cut(s) 1312, 1413
SduI GDGCHC 5 cut(s) 52, 823, 1882, 1924, 2016
SexAI ACCWGGT 1 cut(s) 1610
SfaNI GCATC 6 cut(s) 371, 391, 413, 916, 1739, 2013
SfcI CTRYAG 1 cut(s) 982
SfoI GGCGCC 1 cut(s) 513
Sfr303I CCGCGG 1 cut(s) 11
SgrBI CCGCGG 1 cut(s) 11
SinI GGWCC 3 cut(s) 389, 1076, 1764
SmaI CCCGGG 1 cut(s) 1644
SmiMI CAYNNNNRTG 6 cut(s) 633, 735, 1545, 1892, 2021, 2049
SmlI CTYRAG 1 cut(s) 1923
SmoI CTYRAG 1 cut(s) 1923
SseBI AGGCCT 1 cut(s) 1831
SsiI CCGC 6 cut(s) 8, 10, 90, 1526, 1990, 2117
SspDI GGCGCC 1 cut(s) 511
SspMI CTAG 5 cut(s) 663, 777, 825, 1188, 1200
SstI GAGCTC 1 cut(s) 52
StuI AGGCCT 1 cut(s) 1831
StyI CCWWGG 4 cut(s) 316, 530, 628, 1121
TaaI ACNGT 9 cut(s) 428, 685, 722, 744, 986, 1011, 1076, 1258, 1393
TaiI ACGT 1 cut(s) 78
TaqI TCGA 5 cut(s) 81, 480, 914, 939, 1555
TaqII GACCGA 1 cut(s) 716
TatI WGTACW 2 cut(s) 428, 1230
TauI GCSGC 4 cut(s) 10, 13, 92, 2119
TfiI GAWTC 3 cut(s) 170, 1028, 2000
Tru1I TTAA 6 cut(s) 675, 1128, 1242, 1713, 1802, 1995
Tru9I TTAA 6 cut(s) 675, 1128, 1242, 1713, 1802, 1995
TscAI CASTG 4 cut(s) 234, 334, 1165, 1510
TseFI GTSAC 4 cut(s) 728, 1011, 1156, 1970
TseI GCWGC 8 cut(s) 176, 179, 773, 880, 1163, 1748, 2081, 2123
Tsp45I GTSAC 4 cut(s) 728, 1011, 1156, 1970
TspDTI ATGAA 6 cut(s) 248, 620, 947, 1230, 1751, 2130
TspMI CCCGGG 1 cut(s) 1642
TspRI CASTG 4 cut(s) 234, 334, 1165, 1510
Tth111I GACNNNGTC 1 cut(s) 77
VpaK11BI GGWCC 3 cut(s) 389, 1076, 1764
XapI RAATTY 5 cut(s) 31, 136, 235, 1347, 1567
XceI RCATGY 3 cut(s) 1532, 1902, 2020
XcmI CCANNNNNNNNNTGG 2 cut(s) 290, 1332
XmaI CCCGGG 1 cut(s) 1642
XmiI GTMKAC 1 cut(s) 1815
XmnI GAANNNNTTC 1 cut(s) 580
XspI CTAG 5 cut(s) 663, 777, 825, 1188, 1200
Zsp2I ATGCAT 2 cut(s) 406, 1732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.