pycom03g20780

KIP1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0005688
Physical Location & Seq
Reverse (-)
21210892 .. 21217059
6168 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 5382 bp
ATGGCTACTGCGTCGCAGGCAGACTCTAGACGCAAGTATTCTTGGTGGTGGGACAGCCACATAAGCCCGAAGAATTCAAGATGGCTTCAGGAAAATCTTACAGATATGGATGCCAAAGTCAAACACATGATCAAGCTCATTGAATTGGATGCCGATTCCTTTGCTAGGAGGGCAGAGATGTACTATAAACAGCGCCCAGAATTAATGAAATTGGTAGAAGAGTTCTATCGAGCATACCGTGCATTAGCTGAGAGATATGATCATGCAACTGGGGCACTGCGCCAGGCTCATCGAACCATGGCAGAAGCATTTCCCAACCAAGTTCCCTTTGCAATGGGAGATGATTCACCTGCAGGTTCCTCTGCTAGTGAGGCTGATCCCCGTACACCTGAGATGCCACACCCAATGCGGGCATTCTTAGACCTTGATGAATTGCAAAAAGATGCTCCGGGAATCTCCTCATCCCATTTCCTCAGTGTCAAAAAGAATGGAGCTTACACCAATGAATCTGACTCTGGAACAAGCAGAACGGGCTTGAAACAGCTGAACGATCTATTTGGGTCTGGAGAAGGAAGGGTGAAAAAAGGCCTCAATTTTCATGATGAAGAAGAAAAAGACCGTATGCAGGACAATGAGACCCATAACATCAAGGCTCAATCATTGTCCGAGTCTGATCGACTGGGTAAAGCAGAGACAGAAATTTCAAACTTGAAGGAGGCCCTTGCTAAATTAGAAGCCGAAAAGGAAGCTGGCCTACTTCAGTACCAACAGTGTTTAGAGAGGTTAAATAATCTGGAGTCTGAAGTCGCTCGTGCACATGAGGATTCCAGGGAACTGAACGAGCAGGCCAGTAAAGCTGAAGCTGAAGTTCAAGCTTCGCAGGAAGCACTTGCTAAATTAGAGTCTGAAAGGGATGCTAGCCTTCTGCAGTATCAGCAGTGTTTGGAGAAAATCACTAATCTGGAGGACGGTATCTCTTGTGCCCAAAAGGATGCTGCAGAGCTCAATGATCGTGCGAGTAAAGTTGAAACTGAAGCTGAAACTCTAAAGCAAGATCTTTCAAAGGTCGTAGCTGAGAAAGAAGCTGCACTTGCACAATATCAACAATGTCTAGAAATGATATCAAATCTGGAGGAAAAAATACTGCATATTGAGGAGGATACAAGAAGAATTAATGAGCGAGCTGTTAAAGCCGAAGATGAAGTTGAAACCTTGAAGCAAACAATTGCCAAGTTAAACGAAGAGAAGGAAGCTGCTGCTCTCCAGTACCAGCAGTGCTTGAAAACCATATCTACTCTGGAGCATAAGATAGCTAGTGCCCAAGAGGAGGCCCAACGGCTACACTCTGAAATAGATGATGGGATTGCAAAGTTAAGGGGTTCTGAAGAAAAGTGTATTCTACTGGCACAATCAAATCAGACGCTCCAATCTGAGTTGGAGTCTTTGGTGCAGAAAATGGAATCTCAGGGTGAAGAACTGATTGAGAAGCAGATGGAGTTGGGCAGACTCTGGACTTCCATACGAGAAGAGCGTTTGCGATTCATGGAGGCTGAAACTGCTTTCCAGACACTGCAGCATTTGCATTCTCAATCCCAGGAGGAACTTAGATCTATGTATTCTGAACTTCACAATGGAGCTTTAATTATGAAGGACTTGGAAATGCATAATCAGGTTTTGGAGGATGAAGTCCAGAATTCGAAGGAGGAAAACAAGAGGCTGAGTGAGCTCAATCTGTCTTCATCCATGTCAATAAAAAATCTGCAAGATGAGATCTTAATCTTGAGGGAGACAATTAGAAAACTTGAAGAGGAACTCGAACTCCGAGTAGACCAAAGAAACGCTCTCCAGCAAGAGATATATTGCCTGAAAGAGGAACTCAATGGCTTGAACAAGAAACACCAAGTTATGCTAGAGCAGGTAGACTCAGTTGGCTTGAATCCAGAGTGCCTTGCGTCATCTGTGAAGGAACTGCAGGGTGAAAAATCACAGCTAGAACAGATGTGTGAGGCTGATAGAAGTGAGAAAGCGGCACTGTTAGAAAAGTTGGAAATCATGCAGAAACTTATGGAGAAAAATGTTCTTTTGGAGAGTTCCCTTTCTGATTTGAACCTTGAGTTAGAAGGTGTTAGAGGGAAGGTAAAGGAGTTGGAAGAATCATGTCAATCTCATCTGGAAGAAAAAGGTACAATTGCTGCTGAAAATGCCTCCCTACTTTCCCAGTTACAAATTATGACCGAGAACTTGAAGAAATCATCGGAGAATAACAAAATTTTGGAGAATTCCCTTTGTGATGCAAATGCTGAACTTGAAGGCTTGAGGGTAATATCAAAGAGCTTAGAGGAGTCCTGTCTATTGCTTAATAACGAGAAGTCTGGTCTGACCAAGGAGAGAGAAAATGTAGTTTCTGAGTTGGGCGCTACTCAGCAAAGACTGGTAGGTCTGGAAAAAGGATATACAGAAGTAGAAGAGAAACTCTCTGCTCTTGAGAAAGAGAGAGAATCTGCACTTCGTAAAGTGGAAGAGCTACATGTTTTCTTAAATTCTGAGAAACAAAAACATGCCAGTTTTGTTCAGTTGAGTGAAACCCAGATGGCTGGTATGGGATTGCAGATCTCTCGTCTCCAAGCAGAAGGCATGTGCATGAAGAAAGAATATGAAGTGGAACAAGACAAAGCTGTGAACGCGCAGATTGAAATCTTTGTCTTGCAGAAATGCATAAAAGATCTGGAAGAAAAGATCTTGTCCCTCCTGGTTGAGCGTCAAAAGCTGTTGGAGGCATCCAAAATGTCAGAGAAACAGATTTCTGACCTAGAGCATGGAAATCTTGAGAAACAGGTGGAGATTAAATCTTTCCTTTTGCAAACGAAAGTACTGAGAATGGGGCTGTATCAGGTGTTGAAGACTCTTGACGTGGAGGCAAACCTCAGTTGTGCAGGAGAGGTTGAGCAAGACGAAACACTTCTTAACAACATTCTTGTCAAACTTCAAGAGACACAAAATTCTCTCTCTGAAACTTGTGATCAAAATCAGCAGCTGGTTATTGAGAAGTCAGTTCTCATAGAAATGATTGACCAACTAAAACTAGAGGCAGCAAATCTTATGAGAGAAAGAAACACCCTGGACGGAGAGTTCAAGAATCAGTCTGAGAAGCTTGTGGTGTTACAGAGTGGGGCCCAAAGACTTGAGGAGAATAATGAAGAATTGAAGTTGAAAGTAGAGGAGGGAGACCACAGAGAGCAAGTGTTGAGGACTGAAATAGATGATCTGCATGAGCAGTTCTTGGACTTGCAAAGTGCATACAACGATGTATTGGAAGAGAATGGCAAGATGCTTGTAGAGAAAGGAGCTTTGACAAAGATGGTATCGAATTTGTGGGAAGAGAATCGTGACCTCGAAGAGGAGAAATGTGTCATGTTTGGTGAAACGATATATCACAATAACTTTTCACTTGTTCTCAAAGATTTTATTTCAAGAAAGCTGCTGGAACTGGAAGAGCTCACTGACTATTTAGATAAACTTCATCTTGGAAAGAATGACCTGGAAGATAAGGTAAGGATATTGGAGGGGAAGTTAGAAGATACACGGATGGACAACTTACAGCTCAAGGAGTCATTGAATAAGTCCGAGAATGAGCTGGAGTTGGTTAAATCTGTTAATGATCAATTGAATGGTGAAATTGCAAATGCAAAGGATGTGTTGTACCATAAAGAAAATGAGCTTTTGGAAGTACATCAGGCCATCAGTGTGCTACAGAATGAGAGGCAAGAGTTGCATGCGTTAGTGGAGGACATGAATGGTAAGTATGGTGAGGCTAAGGTGGCACTACAAGATCAAGAGAAGCAAATTCTCAAACTCTCTGCAGACAATGAACTTCATATCAAAGAGACTGGCAACCTTTGTGTAGTGAATCAGGAGTTGGAGTCAGAAGTGCAGAAAATGCAGCAAAAAGCTGAAAAAACTAAAATCAAAGAGGAAGGTTTGATCAATGAACTGCAAAAGGAAAGACAAGAGATAGAAATGTGGTTGTTTCAGGCTGCCACATTATTTGGCGAACTGCAGAGTTCCAACATTCGTGAAACATTGTTTGAAGGAAAGATTCGCGAGCTCATTGAAGCATGTCAGATCCTCGAAGACGGAAGCAATTCCAGAGGCATCGAGAACAAAATTTTGAAAGAAAGAGTTTGCGCATTGGAAGACGAAAATGGAGGGCTACAAGCTCAGTTGGCTGCATATATTCCAGCTGTCATCTCTTTGAAGGAGTGTATAACATCACTTGAGAAGCATATACTTGCAGACACCGGTTCTCATAAACTTGACACTGAAGAATCAAAGGATGCCCTTCTGCATGCTGAAAGCTCTCAAATGGATGGAGATCAAACAGCCACAGTATCAGATGGTGTTTTGGACTTGCAGGAGTTACAAAGAAGAATTGAAGCTATTGAAAAGGCAGTGGTGGAAAAGGAAGGTCATGTTTCTGCAAATCAGGTTCATGACACTATGAAGAAGCGTGAAATATCTGGATCAGGGAATGAAGTTCTGACAAAAGACATCGTTCTTGATCAGATATCGGAGTGTTCATCCTATGGGATAAGCAGAAGAGAAACCATAGAGCCTGATGCTCAGATGCTTGAGTTGTGGGAGACCAGTGACCAGGATGGCAGCATTGATTTGACGGTTGGCAACTCCCAGAAGGCGGCCGCTGTACCCACTGACCACAGTCAGATGGAGGCAGGAAAGGAGCATAATAAAGGACATCCCTCTTCTGAATCATTGGTTGAGAAAGAATTGGGTATTAACAAATTAGAGCTCTCTAGGAGATTTACACAGCCCCGTCAAGAAGGGAACAAGAGAAGCATCCTAGAAAGACTTGATTCCGATGTGCAAAAGTTGACAAACCTTCAAATTACTGTGGAAGACCTGAAGACAAAGGTGGAGATTACTGAGAAGAGCAAAAGGGGCAATAATGTGGAACTTGATAGCGTAAAGGGGCAGCTGGAAGAAGCCAAAGAGGCCATTACGAAGTTGTTTGATGCCAATCAAATGTTGATGAAGAGCGTTGAAGATGATCCTCCGTCCTCTGCTGGATCTTCTGGGGAAGTACCAGATGAAAGTGGGAGCGTGAGAAGGAGGAGACTTTCTGAACAAGCAAAAAGAGGGTCGGAAAACATTGGGCGGTTGCAGTTGCAGGTTCAGAAACTGCAGTTTCTTCTTCTGAAAATCGATGGCGAAAAAGACAGCAAAGGATCAGCTAGAATCATCGAGCGGAAGAAAAGCGTTCTTCTACGTGACTACCTCTATGGCGTGGGAAAACCCGTCAACCAAGGGAAACGGAAGAAATCGCCCTTCTGTGCATGCATACAGCCCCCAACCAAGGGAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIP1

Protein Analysis

1794

Amino Acids

203.4

Weight (kDa)

4.91

Isoelectric Point (pI)

54.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIP1 PF07765 14 - 87 2.9e-36 KIP1-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 358
AasI GACNNNNNNGTC 1 cut(s) 2436
Acc16I TGCGCA 1 cut(s) 4186
Acc36I ACCTGC 4 cut(s) 344, 358, 1906, 5176
AccBSI CCGCTC 1 cut(s) 5263
AccI GTMKAC 2 cut(s) 1827, 1920
AccII CGCG 2 cut(s) 2684, 4101
AciI CCGC 6 cut(s) 409, 2027, 4694, 4698, 5173, 5263
AclWI GGATC 6 cut(s) 371, 4117, 4528, 5060, 5092, 5251
AcoI YGGCCR 1 cut(s) 4695
AcuI CTGAAG 9 cut(s) 71, 743, 822, 879, 885, 1053, 1404, 4341, 4940
AfiI CCNNNNNNNGG 9 cut(s) 165, 409, 625, 1327, 1870, 3397, 4693, 5371, 5372
AflIII ACRYGT 1 cut(s) 2527
AgeI ACCGGT 1 cut(s) 4298
AjiI CACGTC 1 cut(s) 2911
AjnI CCWGG 7 cut(s) 282, 827, 1593, 2748, 3117, 3537, 4650
AjuI GAANNNNNNNTTGG 2 cut(s) 3899, 3931
AloI GAACNNNNNNTCC 4 cut(s) 1803, 1835, 3113, 3145
Alw21I GWGCWC 6 cut(s) 817, 1005, 1728, 3498, 4107, 4809
Alw26I GTCTC 9 cut(s) 629, 686, 1781, 2624, 2984, 3219, 3878, 4634, 5125
Alw44I GTGCAC 1 cut(s) 813
AlwI GGATC 6 cut(s) 371, 4117, 4528, 5060, 5092, 5251
AlwNI CAGNNNCTG 4 cut(s) 1509, 1570, 3034, 5197
AoxI GGCC 9 cut(s) 586, 717, 751, 846, 1329, 3171, 3735, 4695, 5010
ApaI GGGCCC 1 cut(s) 3175
ApaLI GTGCAC 1 cut(s) 813
ArsI GACNNNNNNTTYG 2 cut(s) 5141, 5173
AseI ATTAAT 2 cut(s) 203, 1173
AsiGI ACCGGT 1 cut(s) 4298
Asp700I GAANNNNTTC 3 cut(s) 309, 2958, 4141
AspLEI GCGC 5 cut(s) 195, 282, 2417, 2686, 4187
AspS9I GGNCC 4 cut(s) 718, 1330, 3171, 3172
AsuC2I CCSGG 1 cut(s) 450
AsuHPI GGTGA 7 cut(s) 339, 589, 1481, 1988, 3431, 3683, 3818
AsuII TTCGAA 1 cut(s) 1697
AsuNHI GCTAGC 1 cut(s) 917
BaeGI GKGCMC 5 cut(s) 277, 817, 985, 1321, 3175
BaeI ACNNNNGTAYC 2 cut(s) 746, 779
BanII GRGCYC 6 cut(s) 1005, 1728, 3175, 3498, 4107, 4809
BarI GAAGNNNNNNTAC 2 cut(s) 738, 770
BauI CACGAG 1 cut(s) 810
BbsI GAAGAC 6 cut(s) 1728, 2906, 4137, 4200, 4920, 4928
Bbv12I GWGCWC 6 cut(s) 817, 1005, 1728, 3498, 4107, 4809
BceAI ACGGC 1 cut(s) 1352
BcgI CGANNNNNNTGC 4 cut(s) 143, 177, 2597, 2631
BciT130I CCWGG 7 cut(s) 284, 829, 1595, 2750, 3119, 3539, 4652
BciVI GTATCC 1 cut(s) 1153
BclI TGATCA 6 cut(s) 129, 259, 3019, 3658, 3981, 4558
BcnI CCSGG 1 cut(s) 450
BcoDI GTCTC 9 cut(s) 629, 686, 1781, 2624, 2984, 3219, 3878, 4634, 5125
BfmI CTRYAG 9 cut(s) 351, 926, 996, 1571, 1970, 3749, 3858, 4055, 5198
BfoI RGCGCY 2 cut(s) 196, 2418
BfuAI ACCTGC 4 cut(s) 344, 358, 1906, 5176
BfuI GTATCC 1 cut(s) 1153
BglI GCCNNNNNGGC 1 cut(s) 5009
BglII AGATCT 6 cut(s) 1054, 1607, 1770, 2610, 2722, 2736
BmcAI AGTACT 1 cut(s) 2871
Bme1390I CCNGG 8 cut(s) 284, 450, 829, 1595, 2750, 3119, 3539, 4652
BmgBI CACGTC 1 cut(s) 2911
BmgT120I GGNCC 4 cut(s) 718, 1330, 3171, 3172
BmiI GGNNCC 3 cut(s) 358, 3172, 3173
BmrFI CCNGG 8 cut(s) 284, 450, 829, 1595, 2750, 3119, 3539, 4652
BmrI ACTGGG 3 cut(s) 279, 689, 2212
BmtI GCTAGC 1 cut(s) 921
BmuI ACTGGG 3 cut(s) 279, 689, 2212
BoxI GACNNNNGTC 1 cut(s) 4716
BpiI GAAGAC 6 cut(s) 1728, 2906, 4137, 4200, 4920, 4928
BplI GAGNNNNNCTC 2 cut(s) 2459, 2491
BpmI CTGGAG 8 cut(s) 585, 815, 983, 1151, 1247, 1319, 1829, 3656
Bpu10I CCTNAGC 1 cut(s) 3813
Bpu14I TTCGAA 1 cut(s) 1697
BpuEI CTTGAG 9 cut(s) 1801, 2132, 2335, 2504, 2846, 3203, 3587, 4295, 4649
BpuMI CCSGG 1 cut(s) 450
Bsa29I ATCGAT 1 cut(s) 5220
BsaAI YACGTR 1 cut(s) 5285
BsaBI GATNNNNATC 5 cut(s) 1775, 2693, 3024, 4371, 5034
BsaI GGTCTC 3 cut(s) 629, 3219, 4634
BsaJI CCNNGG 7 cut(s) 297, 828, 1593, 2382, 3117, 5320, 5370
BsaWI WCCGGW 1 cut(s) 4298
BsaXI ACNNNNNCTCC 6 cut(s) 1803, 1833, 3554, 3584, 3905, 3935
Bsc4I CCNNNNNNNGG 9 cut(s) 165, 409, 625, 1327, 1870, 3397, 4693, 5371, 5372
Bse118I RCCGGY 1 cut(s) 4298
Bse3DI GCAATG 1 cut(s) 339
Bse8I GATNNNNATC 5 cut(s) 1775, 2693, 3024, 4371, 5034
BseBI CCWGG 7 cut(s) 284, 829, 1595, 2750, 3119, 3539, 4652
BseCI ATCGAT 1 cut(s) 5220
BseDI CCNNGG 7 cut(s) 297, 828, 1593, 2382, 3117, 5320, 5370
BseJI GATNNNNATC 5 cut(s) 1775, 2693, 3024, 4371, 5034
BseLI CCNNNNNNNGG 9 cut(s) 165, 409, 625, 1327, 1870, 3397, 4693, 5371, 5372
BseMI GCAATG 1 cut(s) 339
BseRI GAGGAG 8 cut(s) 448, 1169, 1340, 2354, 3200, 3233, 3413, 5143
BseSI GKGCMC 5 cut(s) 277, 817, 985, 1321, 3175
BseX3I CGGCCG 1 cut(s) 4695
BsgI GTGCAG 5 cut(s) 1071, 1469, 2487, 2952, 3950
Bsh1236I CGCG 2 cut(s) 2684, 4101
Bsh1285I CGRYCG 1 cut(s) 4698
BshFI GGCC 9 cut(s) 588, 719, 753, 848, 1331, 3173, 3737, 4697, 5012
BshTI ACCGGT 1 cut(s) 4298
BshVI ATCGAT 1 cut(s) 5220
BsiEI CGRYCG 1 cut(s) 4698
BsiHKAI GWGCWC 6 cut(s) 817, 1005, 1728, 3498, 4107, 4809
BsiSI CCGG 2 cut(s) 449, 4299
BslFI GGGAC 2 cut(s) 65, 2728
BslI CCNNNNNNNGG 9 cut(s) 165, 409, 625, 1327, 1870, 3397, 4693, 5371, 5372
BsmAI GTCTC 9 cut(s) 629, 686, 1781, 2624, 2984, 3219, 3878, 4634, 5125
BsmBI CGTCTC 1 cut(s) 2624
BsmFI GGGAC 2 cut(s) 65, 2728
BsmI GAATGC 2 cut(s) 413, 1582
BsnI GGCC 9 cut(s) 588, 719, 753, 848, 1331, 3173, 3737, 4697, 5012
Bso31I GGTCTC 3 cut(s) 629, 3219, 4634
Bsp119I TTCGAA 1 cut(s) 1697
Bsp120I GGGCCC 1 cut(s) 3171
Bsp19I CCATGG 1 cut(s) 297
Bsp68I TCGCGA 1 cut(s) 4101
BspACI CCGC 6 cut(s) 409, 2027, 4694, 4698, 5173, 5263
BspANI GGCC 9 cut(s) 588, 719, 753, 848, 1331, 3173, 3737, 4697, 5012
BspDI ATCGAT 1 cut(s) 5220
BspFNI CGCG 2 cut(s) 2684, 4101
BspHI TCATGA 2 cut(s) 598, 4489
BspLI GGNNCC 3 cut(s) 358, 3172, 3173
BspMAI CTGCAG 8 cut(s) 355, 930, 1000, 1575, 1974, 3862, 4059, 5202
BspMI ACCTGC 4 cut(s) 344, 358, 1906, 5176
BspOI GCTAGC 1 cut(s) 921
BspPI GGATC 6 cut(s) 371, 4117, 4528, 5060, 5092, 5251
BspQI GCTCTTC 5 cut(s) 1521, 2514, 3486, 4940, 5045
BspT104I TTCGAA 1 cut(s) 1697
BspTNI GGTCTC 3 cut(s) 629, 3219, 4634
BsrBI CCGCTC 1 cut(s) 5263
BsrDI GCAATG 1 cut(s) 339
BsrFI RCCGGY 1 cut(s) 4298
BssAI RCCGGY 1 cut(s) 4298
BssECI CCNNGG 7 cut(s) 297, 828, 1593, 2382, 3117, 5320, 5370
BssSI CACGAG 1 cut(s) 810
BssT1I CCWWGG 4 cut(s) 297, 2382, 5320, 5370
Bst2BI CACGAG 1 cut(s) 810
Bst2UI CCWGG 7 cut(s) 284, 829, 1595, 2750, 3119, 3539, 4652
Bst4CI ACNGT 9 cut(s) 239, 620, 771, 971, 2034, 4387, 4675, 4718, 4909
BstAPI GCANNNNNTGC 4 cut(s) 239, 1579, 3769, 3937
BstBAI YACGTR 1 cut(s) 5285
BstBI TTCGAA 1 cut(s) 1697
BstDSI CCRYGG 1 cut(s) 297
BstENI CCTNNNNNAGG 3 cut(s) 163, 1868, 3395
BstFNI CGCG 2 cut(s) 2684, 4101
BstH2I RGCGCY 2 cut(s) 196, 2418
BstHHI GCGC 5 cut(s) 195, 282, 2417, 2686, 4187
BstMAI GTCTC 9 cut(s) 629, 686, 1781, 2624, 2984, 3219, 3878, 4634, 5125
BstMCI CGRYCG 1 cut(s) 4698
BstNI CCWGG 7 cut(s) 284, 829, 1595, 2750, 3119, 3539, 4652
BstNSI RCATGY 7 cut(s) 2531, 2561, 2638, 3776, 4119, 4349, 5355
BstPAI GACNNNNGTC 1 cut(s) 4716
BstSCI CCNGG 8 cut(s) 282, 448, 827, 1593, 2748, 3117, 3537, 4650
BstSFI CTRYAG 9 cut(s) 351, 926, 996, 1571, 1970, 3749, 3858, 4055, 5198
BstSLI GKGCMC 5 cut(s) 277, 817, 985, 1321, 3175
BstUI CGCG 2 cut(s) 2684, 4101
BstV2I GAAGAC 6 cut(s) 1728, 2906, 4137, 4200, 4920, 4928
BstX2I RGATCY 8 cut(s) 1054, 1607, 1770, 2610, 2722, 2736, 4122, 5084
BstXI CCANNNNNNTGG 1 cut(s) 2594
BstYI RGATCY 8 cut(s) 1054, 1607, 1770, 2610, 2722, 2736, 4122, 5084
BstZI CGGCCG 1 cut(s) 4695
Bsu15I ATCGAT 1 cut(s) 5220
BsuI GTATCC 1 cut(s) 1153
BsuRI GGCC 9 cut(s) 588, 719, 753, 848, 1331, 3173, 3737, 4697, 5012
BsuTUI ATCGAT 1 cut(s) 5220
BtgI CCRYGG 1 cut(s) 297
BtrI CACGTC 1 cut(s) 2911
BtsI GCAGTG 5 cut(s) 275, 944, 1280, 1568, 4455
BtuMI TCGCGA 1 cut(s) 4101
BveI ACCTGC 4 cut(s) 344, 358, 1906, 5176
CaiI CAGNNNCTG 4 cut(s) 1509, 1570, 3034, 5197
CciI TCATGA 2 cut(s) 598, 4489
CciNI GCGGCCGC 1 cut(s) 4695
CfoI GCGC 5 cut(s) 195, 282, 2417, 2686, 4187
Cfr10I RCCGGY 1 cut(s) 4298
Cfr13I GGNCC 4 cut(s) 718, 1330, 3171, 3172
ClaI ATCGAT 1 cut(s) 5220
CseI GACGC 4 cut(s) 39, 1429, 1941, 2747
CspAI ACCGGT 1 cut(s) 4298
CspCI CAANNNNNGTGG 2 cut(s) 4027, 4062
DrdI GACNNNNNNGTC 1 cut(s) 2436
DseDI GACNNNNNNGTC 1 cut(s) 2436
EaeI YGGCCR 1 cut(s) 4695
EagI CGGCCG 1 cut(s) 4695
Ecl136II GAGCTC 5 cut(s) 1003, 1726, 3496, 4105, 4807
EclXI CGGCCG 1 cut(s) 4695
Eco130I CCWWGG 4 cut(s) 297, 2382, 5320, 5370
Eco147I AGGCCT 1 cut(s) 588
Eco24I GRGCYC 6 cut(s) 1005, 1728, 3175, 3498, 4107, 4809
Eco31I GGTCTC 3 cut(s) 629, 3219, 4634
Eco32I GATATC 2 cut(s) 1122, 4566
Eco52I CGGCCG 1 cut(s) 4695
Eco53kI GAGCTC 5 cut(s) 1003, 1726, 3496, 4105, 4807
Eco57I CTGAAG 9 cut(s) 71, 743, 822, 879, 885, 1053, 1404, 4341, 4940
EcoICRI GAGCTC 5 cut(s) 1003, 1726, 3496, 4105, 4807
EcoNI CCTNNNNNAGG 3 cut(s) 163, 1868, 3395
EcoO109I RGGNCCY 2 cut(s) 718, 3171
EcoRI GAATTC 3 cut(s) 73, 1693, 2278
EcoRII CCWGG 7 cut(s) 282, 827, 1593, 2748, 3117, 3537, 4650
EcoRV GATATC 2 cut(s) 1122, 4566
EcoT14I CCWWGG 4 cut(s) 297, 2382, 5320, 5370
EcoT22I ATGCAT 3 cut(s) 1665, 2717, 5357
EcoT38I GRGCYC 6 cut(s) 1005, 1728, 3175, 3498, 4107, 4809
ErhI CCWWGG 4 cut(s) 297, 2382, 5320, 5370
Esp3I CGTCTC 1 cut(s) 2624
FalI AAGNNNNNCTT 2 cut(s) 1074, 1106
FaqI GGGAC 2 cut(s) 65, 2728
FauI CCCGC 1 cut(s) 402
FbaI TGATCA 6 cut(s) 129, 259, 3019, 3658, 3981, 4558
FblI GTMKAC 2 cut(s) 1827, 1920
FriOI GRGCYC 6 cut(s) 1005, 1728, 3175, 3498, 4107, 4809
FspI TGCGCA 1 cut(s) 4186
GlaI GCGC 5 cut(s) 194, 281, 2416, 2685, 4186
GsuI CTGGAG 8 cut(s) 585, 815, 983, 1151, 1247, 1319, 1829, 3656
HaeII RGCGCY 2 cut(s) 196, 2418
HaeIII GGCC 9 cut(s) 588, 719, 753, 848, 1331, 3173, 3737, 4697, 5012
HapII CCGG 2 cut(s) 449, 4299
HgaI GACGC 4 cut(s) 39, 1429, 1941, 2747
HhaI GCGC 5 cut(s) 195, 282, 2417, 2686, 4187
Hin6I GCGC 5 cut(s) 193, 280, 2415, 2684, 4185
HinP1I GCGC 5 cut(s) 193, 280, 2415, 2684, 4185
HincII GTYRAC 2 cut(s) 4890, 5317
HindII GTYRAC 2 cut(s) 4890, 5317
HindIII AAGCTT 2 cut(s) 873, 3149
HpaII CCGG 2 cut(s) 449, 4299
HphI GGTGA 7 cut(s) 339, 589, 1481, 1988, 3431, 3683, 3818
Hpy166II GTNNAC 7 cut(s) 386, 815, 1828, 1921, 2680, 4890, 5317
Hpy8I GTNNAC 7 cut(s) 386, 815, 1828, 1921, 2680, 4890, 5317
Hpy99I CGWCG 1 cut(s) 16
HpyCH4III ACNGT 9 cut(s) 239, 620, 771, 971, 2034, 4387, 4675, 4718, 4909
HpyCH4IV ACGT 2 cut(s) 2910, 5284
HpySE526I ACGT 2 cut(s) 2910, 5284
HspAI GCGC 5 cut(s) 193, 280, 2415, 2684, 4185
Ksp22I TGATCA 6 cut(s) 129, 259, 3019, 3658, 3981, 4558
LguI GCTCTTC 5 cut(s) 1521, 2514, 3486, 4940, 5045
LmnI GCTCC 8 cut(s) 451, 491, 1300, 1428, 1634, 3344, 4738, 5115
MaeII ACGT 2 cut(s) 2910, 5284
MaeIII GTNAC 6 cut(s) 2220, 3159, 3386, 4416, 4646, 5285
MbiI CCGCTC 1 cut(s) 5263
MfeI CAATTG 3 cut(s) 1224, 2187, 3662
MflI RGATCY 8 cut(s) 1054, 1607, 1770, 2610, 2722, 2736, 4122, 5084
MmeI TCCRAC 7 cut(s) 1416, 2025, 2127, 2751, 3897, 4089, 5139
Mph1103I ATGCAT 3 cut(s) 1665, 2717, 5357
MroXI GAANNNNTTC 3 cut(s) 309, 2958, 4141
MslI CAYNNNNRTG 3 cut(s) 2639, 3062, 3743
MspA1I CMGCKG 5 cut(s) 544, 3034, 4241, 4700, 4993
MspI CCGG 2 cut(s) 449, 4299
MspR9I CCNGG 8 cut(s) 284, 450, 829, 1595, 2750, 3119, 3539, 4652
MunI CAATTG 3 cut(s) 1224, 2187, 3662
Mva1269I GAATGC 2 cut(s) 413, 1582
MvaI CCWGG 7 cut(s) 284, 829, 1595, 2750, 3119, 3539, 4652
MvnI CGCG 2 cut(s) 2684, 4101
NciI CCSGG 1 cut(s) 450
NcoI CCATGG 1 cut(s) 297
NheI GCTAGC 1 cut(s) 917
NlaIV GGNNCC 3 cut(s) 358, 3172, 3173
NmuCI GTSAC 3 cut(s) 3386, 4646, 5285
NotI GCGGCCGC 1 cut(s) 4695
NruI TCGCGA 1 cut(s) 4101
NsbI TGCGCA 1 cut(s) 4186
NsiI ATGCAT 3 cut(s) 1665, 2717, 5357
NspI RCATGY 7 cut(s) 2531, 2561, 2638, 3776, 4119, 4349, 5355
NspV TTCGAA 1 cut(s) 1697
PaeI GCATGC 3 cut(s) 3776, 4349, 5355
PagI TCATGA 2 cut(s) 598, 4489
PaqCI CACCTGC 1 cut(s) 358
PceI AGGCCT 1 cut(s) 588
PciI ACATGT 1 cut(s) 2527
PciSI GCTCTTC 5 cut(s) 1521, 2514, 3486, 4940, 5045
PcsI WCGNNNNNNNCGW 2 cut(s) 235, 1528
PctI GAATGC 2 cut(s) 413, 1582
PdmI GAANNNNTTC 3 cut(s) 309, 2958, 4141
PfoI TCCNGGA 1 cut(s) 448
PinAI ACCGGT 1 cut(s) 4298
Ppu21I YACGTR 1 cut(s) 5285
PscI ACATGT 1 cut(s) 2527
PshAI GACNNNNGTC 1 cut(s) 4716
PshBI ATTAAT 2 cut(s) 203, 1173
Psp124BI GAGCTC 5 cut(s) 1005, 1728, 3498, 4107, 4809
Psp6I CCWGG 7 cut(s) 282, 827, 1593, 2748, 3117, 3537, 4650
PspGI CCWGG 7 cut(s) 282, 827, 1593, 2748, 3117, 3537, 4650
PspN4I GGNNCC 3 cut(s) 358, 3172, 3173
PspOMI GGGCCC 1 cut(s) 3171
PspPI GGNCC 4 cut(s) 718, 1330, 3171, 3172
PstI CTGCAG 8 cut(s) 355, 930, 1000, 1575, 1974, 3862, 4059, 5202
PstNI CAGNNNCTG 4 cut(s) 1509, 1570, 3034, 5197
PsuI RGATCY 8 cut(s) 1054, 1607, 1770, 2610, 2722, 2736, 4122, 5084
PvuII CAGCTG 4 cut(s) 544, 3034, 4241, 4993
RruI TCGCGA 1 cut(s) 4101
RseI CAYNNNNRTG 3 cut(s) 2639, 3062, 3743
SacI GAGCTC 5 cut(s) 1005, 1728, 3498, 4107, 4809
SapI GCTCTTC 5 cut(s) 1521, 2514, 3486, 4940, 5045
Sau96I GGNCC 4 cut(s) 718, 1330, 3171, 3172
SbfI CCTGCAGG 1 cut(s) 355
ScaI AGTACT 1 cut(s) 2871
ScrFI CCNGG 8 cut(s) 284, 450, 829, 1595, 2750, 3119, 3539, 4652
SdaI CCTGCAGG 1 cut(s) 355
SfcI CTRYAG 9 cut(s) 351, 926, 996, 1571, 1970, 3749, 3858, 4055, 5198
SfuI TTCGAA 1 cut(s) 1697
SmiMI CAYNNNNRTG 3 cut(s) 2639, 3062, 3743
SmlI CTYRAG 9 cut(s) 1780, 2111, 2314, 2483, 2825, 3182, 3602, 4274, 4628
SmoI CTYRAG 9 cut(s) 1780, 2111, 2314, 2483, 2825, 3182, 3602, 4274, 4628
SphI GCATGC 3 cut(s) 3776, 4349, 5355
Sse8387I CCTGCAGG 1 cut(s) 355
SseBI AGGCCT 1 cut(s) 588
SsiI CCGC 6 cut(s) 409, 2027, 4694, 4698, 5173, 5263
SstI GAGCTC 5 cut(s) 1005, 1728, 3498, 4107, 4809
StuI AGGCCT 1 cut(s) 588
StyD4I CCNGG 8 cut(s) 282, 448, 827, 1593, 2748, 3117, 3537, 4650
StyI CCWWGG 4 cut(s) 297, 2382, 5320, 5370
TaaI ACNGT 9 cut(s) 239, 620, 771, 971, 2034, 4387, 4675, 4718, 4909
TaiI ACGT 2 cut(s) 2913, 5287
TaqII GACCGA 1 cut(s) 2249
TatI WGTACW 3 cut(s) 180, 2869, 3727
TauI GCSGC 3 cut(s) 2030, 4697, 4700
TseFI GTSAC 3 cut(s) 3386, 4646, 5285
Tsp45I GTSAC 3 cut(s) 3386, 4646, 5285
TspGWI ACGGA 5 cut(s) 3138, 3598, 4149, 5061, 5344
VneI GTGCAC 1 cut(s) 813
VspI ATTAAT 2 cut(s) 203, 1173
XagI CCTNNNNNAGG 3 cut(s) 163, 1868, 3395
XbaI TCTAGA 2 cut(s) 26, 1111
XceI RCATGY 7 cut(s) 2531, 2561, 2638, 3776, 4119, 4349, 5355
XcmI CCANNNNNNNNNTGG 1 cut(s) 1294
XmiI GTMKAC 2 cut(s) 1827, 1920
XmnI GAANNNNTTC 3 cut(s) 309, 2958, 4141
ZrmI AGTACT 1 cut(s) 2871
Zsp2I ATGCAT 3 cut(s) 1665, 2717, 5357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.