pycom04g03690

Protein FAR1-RELATED SEQUENCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0009654
Physical Location & Seq
Reverse (-)
3299088 .. 3300688
1601 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 723 bp
ATGCATGCTGAAACCTATGAACATAATGAAGGGATAGAAGATGTGCTTCCTGAGAATTTGGACTTGTGTGTAGATGAAAAAGAGGAGACAGAAGAACAATCGGCTGATCTACCTATGAACGTAGAAGCTTTAGAACCGTATATAGGCATGGAGTTCAACTCAAGAGATGAAGCTAGAGAATTTTATGTTGCATATGGCAGACGCTCCGGTTTCACCGTACGGATACACCACAATCGTCGTTCACGAGTAAATAATCAGATTATTGGTCAAGATTTTGTTTGCTCAAAAGAAGGCTTTCGAGCAAAAAAGTATGTGCACAGAAAAGACAGAGTTCTTCCTCCACCCCCAGTAACCCGTGAGGGCTGTCAAGCTATGATAAGGCTAGCTCAAAGAGATGGACCAAAGTGGGTAGTCACAAAATTTGTTAAAGAGCATAATCACAAGCTAATGAGTCCCAGCAAGGTTCCATGGCGGGGATCAGGAAAGCACTTAGCAAGTGAGGATGAGAAGGATAAAAGAATCCGAGAGCTGTCCCTTGAGCTGTACAACGAGAGGCAAAAATGTAAGCGACGTTGTGCTGCATATGAAGAACAGCTAAATATAATCTTGAAAGATTTGGAAAAACACACAGAGCACATGTCAAAAAAAGTTTCGGATACAGTTCAGAGCATAAAAGAAATTGAGGAGGAACAATCAGAGGACTCGGATAGCGGATGGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAR1

Protein Analysis

241

Amino Acids

28.1

Weight (kDa)

5.98

Isoelectric Point (pI)

68.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAR1 PF03101 60 - 150 3.9e-26 FAR1 DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 472, 711
AclWI GGATC 1 cut(s) 484
AcsI RAATTY 3 cut(s) 55, 179, 419
AfaI GTAC 2 cut(s) 219, 545
AfiI CCNNNNNNNGG 2 cut(s) 143, 473
AflIII ACRYGT 1 cut(s) 636
AgsI TTSAA 2 cut(s) 157, 610
AluBI AGCT 8 cut(s) 128, 173, 371, 386, 445, 529, 541, 595
AluI AGCT 8 cut(s) 128, 173, 371, 386, 445, 529, 541, 595
Alw21I GWGCWC 2 cut(s) 318, 636
Alw26I GTCTC 1 cut(s) 80
Alw44I GTGCAC 1 cut(s) 314
AlwI GGATC 1 cut(s) 484
ApaLI GTGCAC 1 cut(s) 314
ApeKI GCWGC 1 cut(s) 578
ApoI RAATTY 3 cut(s) 55, 179, 419
Asp700I GAANNNNTTC 1 cut(s) 294
AspS9I GGNCC 1 cut(s) 398
AsuHPI GGTGA 1 cut(s) 205
AsuNHI GCTAGC 1 cut(s) 382
AvaII GGWCC 1 cut(s) 398
BaeGI GKGCMC 1 cut(s) 318
BauI CACGAG 1 cut(s) 243
Bbv12I GWGCWC 2 cut(s) 318, 636
BbvI GCAGC 1 cut(s) 565
BccI CCATC 2 cut(s) 389, 708
BciVI GTATCC 2 cut(s) 216, 649
BcoDI GTCTC 1 cut(s) 80
BfaI CTAG 2 cut(s) 174, 383
BfuI GTATCC 2 cut(s) 216, 649
BisI GCNGC 1 cut(s) 579
BlsI GCNGC 1 cut(s) 580
Bme18I GGWCC 1 cut(s) 398
BmgT120I GGNCC 1 cut(s) 398
BmiI GGNNCC 1 cut(s) 465
BmrI ACTGGG 1 cut(s) 341
BmtI GCTAGC 1 cut(s) 386
BmuI ACTGGG 1 cut(s) 341
BplI GAGNNNNNCTC 2 cut(s) 143, 175
BpuEI CTTGAG 2 cut(s) 145, 557
BsaJI CCNNGG 1 cut(s) 467
BsaWI WCCGGW 1 cut(s) 206
Bsc4I CCNNNNNNNGG 2 cut(s) 143, 473
Bse1I ACTGG 1 cut(s) 347
BseDI CCNNGG 1 cut(s) 467
BseGI GGATG 2 cut(s) 508, 719
BseLI CCNNNNNNNGG 2 cut(s) 143, 473
BseMII CTCAG 1 cut(s) 42
BseNI ACTGG 1 cut(s) 347
BseRI GAGGAG 2 cut(s) 98, 698
BseSI GKGCMC 1 cut(s) 318
BseXI GCAGC 1 cut(s) 565
BseYI CCCAGC 1 cut(s) 455
BsiHKAI GWGCWC 2 cut(s) 318, 636
BsiSI CCGG 1 cut(s) 207
BsiWI CGTACG 1 cut(s) 217
BslFI GGGAC 2 cut(s) 438, 517
BslI CCNNNNNNNGG 2 cut(s) 143, 473
BsmAI GTCTC 1 cut(s) 80
BsmFI GGGAC 2 cut(s) 438, 517
Bsp1286I GDGCHC 2 cut(s) 318, 636
Bsp1407I TGTACA 1 cut(s) 543
Bsp143I GATC 2 cut(s) 106, 476
Bsp19I CCATGG 1 cut(s) 467
BspACI CCGC 2 cut(s) 472, 711
BspCNI CTCAG 1 cut(s) 43
BspLI GGNNCC 1 cut(s) 465
BspOI GCTAGC 1 cut(s) 386
BspPI GGATC 1 cut(s) 484
BsrGI TGTACA 1 cut(s) 543
BsrI ACTGG 1 cut(s) 347
BssECI CCNNGG 1 cut(s) 467
BssMI GATC 2 cut(s) 106, 476
BssSI CACGAG 1 cut(s) 243
BssT1I CCWWGG 1 cut(s) 467
Bst2BI CACGAG 1 cut(s) 243
Bst4CI ACNGT 3 cut(s) 138, 217, 661
BstAUI TGTACA 1 cut(s) 543
BstC8I GCNNGC 2 cut(s) 6, 384
BstDEI CTNAG 2 cut(s) 51, 490
BstDSI CCRYGG 1 cut(s) 467
BstF5I GGATG 2 cut(s) 508, 719
BstKTI GATC 2 cut(s) 109, 479
BstMAI GTCTC 1 cut(s) 80
BstMBI GATC 2 cut(s) 106, 476
BstNSI RCATGY 2 cut(s) 8, 640
BstSLI GKGCMC 1 cut(s) 318
BstV1I GCAGC 1 cut(s) 565
BsuI GTATCC 2 cut(s) 216, 649
BtgI CCRYGG 1 cut(s) 467
BtsCI GGATG 2 cut(s) 508, 719
Cac8I GCNNGC 2 cut(s) 6, 384
Cfr13I GGNCC 1 cut(s) 398
CseI GACGC 1 cut(s) 210
Csp6I GTAC 2 cut(s) 218, 544
CviAII CATG 4 cut(s) 5, 148, 468, 637
CviQI GTAC 2 cut(s) 218, 544
DdeI CTNAG 2 cut(s) 51, 490
DpnI GATC 2 cut(s) 108, 478
DpnII GATC 2 cut(s) 106, 476
Eco130I CCWWGG 1 cut(s) 467
Eco47I GGWCC 1 cut(s) 398
EcoT14I CCWWGG 1 cut(s) 467
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 467
FaeI CATG 4 cut(s) 8, 151, 471, 640
FalI AAGNNNNNCTT 2 cut(s) 30, 62
FaqI GGGAC 2 cut(s) 438, 517
FatI CATG 4 cut(s) 4, 147, 467, 636
FauI CCCGC 1 cut(s) 465
FauNDI CATATG 2 cut(s) 193, 583
Fnu4HI GCNGC 1 cut(s) 579
FokI GGATG 1 cut(s) 515
Fsp4HI GCNGC 1 cut(s) 579
FspBI CTAG 2 cut(s) 174, 383
GluI GCNGC 1 cut(s) 579
GsaI CCCAGC 1 cut(s) 459
HapII CCGG 1 cut(s) 207
HgaI GACGC 1 cut(s) 210
Hin1II CATG 4 cut(s) 8, 151, 471, 640
HindIII AAGCTT 1 cut(s) 126
HinfI GANTC 3 cut(s) 451, 519, 701
HpaII CCGG 1 cut(s) 207
HphI GGTGA 1 cut(s) 205
Hpy166II GTNNAC 2 cut(s) 242, 316
Hpy188I TCNGA 6 cut(s) 258, 524, 655, 666, 697, 706
Hpy188III TCNNGA 6 cut(s) 50, 162, 243, 269, 480, 607
Hpy8I GTNNAC 2 cut(s) 242, 316
Hpy99I CGWCG 2 cut(s) 240, 573
HpyAV CCTTC 3 cut(s) 23, 284, 502
HpyCH4III ACNGT 3 cut(s) 138, 217, 661
HpyCH4IV ACGT 2 cut(s) 120, 571
HpyCH4V TGCA 4 cut(s) 4, 191, 316, 581
HpyF3I CTNAG 2 cut(s) 51, 490
HpySE526I ACGT 2 cut(s) 120, 571
Hsp92II CATG 4 cut(s) 8, 151, 471, 640
Kzo9I GATC 2 cut(s) 106, 476
LmnI GCTCC 1 cut(s) 209
LpnPI CCDG 5 cut(s) 63, 220, 360, 465, 469
Lsp1109I GCAGC 1 cut(s) 565
MaeI CTAG 2 cut(s) 174, 383
MaeII ACGT 2 cut(s) 120, 571
MaeIII GTNAC 2 cut(s) 349, 412
MalI GATC 2 cut(s) 108, 478
MboI GATC 2 cut(s) 106, 476
MboII GAAGA 4 cut(s) 50, 104, 326, 599
MhlI GDGCHC 2 cut(s) 318, 636
MluCI AATT 4 cut(s) 55, 179, 419, 678
MlyI GAGTC 2 cut(s) 460, 695
MnlI CCTC 8 cut(s) 76, 348, 352, 493, 546, 676, 679, 691
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 294
MseI TTAA 2 cut(s) 426, 721
MspI CCGG 1 cut(s) 207
NcoI CCATGG 1 cut(s) 467
NdeI CATATG 2 cut(s) 193, 583
NdeII GATC 2 cut(s) 106, 476
NheI GCTAGC 1 cut(s) 382
NlaIII CATG 4 cut(s) 8, 151, 471, 640
NlaIV GGNNCC 1 cut(s) 465
NmuCI GTSAC 1 cut(s) 412
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 8, 640
PaeI GCATGC 1 cut(s) 8
PciI ACATGT 1 cut(s) 636
PcsI WCGNNNNNNNCGW 1 cut(s) 241
PdmI GAANNNNTTC 1 cut(s) 294
PfeI GAWTC 1 cut(s) 519
Pfl23II CGTACG 1 cut(s) 217
PkrI GCNGC 1 cut(s) 580
PleI GAGTC 2 cut(s) 459, 695
PpsI GAGTC 2 cut(s) 459, 695
PscI ACATGT 1 cut(s) 636
PspFI CCCAGC 1 cut(s) 455
PspLI CGTACG 1 cut(s) 217
PspN4I GGNNCC 1 cut(s) 465
PspPI GGNCC 1 cut(s) 398
RsaI GTAC 2 cut(s) 219, 545
RsaNI GTAC 2 cut(s) 218, 544
SaqAI TTAA 2 cut(s) 426, 721
SatI GCNGC 1 cut(s) 579
Sau3AI GATC 2 cut(s) 106, 476
Sau96I GGNCC 1 cut(s) 398
SchI GAGTC 2 cut(s) 460, 695
SduI GDGCHC 2 cut(s) 318, 636
SinI GGWCC 1 cut(s) 398
SmlI CTYRAG 2 cut(s) 160, 536
SmoI CTYRAG 2 cut(s) 160, 536
SphI GCATGC 1 cut(s) 8
Sse9I AATT 4 cut(s) 55, 179, 419, 678
SsiI CCGC 2 cut(s) 472, 711
SspMI CTAG 2 cut(s) 174, 383
StyI CCWWGG 1 cut(s) 467
TaaI ACNGT 3 cut(s) 138, 217, 661
TaiI ACGT 2 cut(s) 123, 574
TaqI TCGA 1 cut(s) 298
TasI AATT 4 cut(s) 55, 179, 419, 678
TatI WGTACW 1 cut(s) 543
TfiI GAWTC 1 cut(s) 519
Tru1I TTAA 2 cut(s) 426, 721
Tru9I TTAA 2 cut(s) 426, 721
TseFI GTSAC 1 cut(s) 412
TseI GCWGC 1 cut(s) 578
Tsp45I GTSAC 1 cut(s) 412
TspDTI ATGAA 6 cut(s) 33, 42, 90, 131, 183, 600
TspGWI ACGGA 1 cut(s) 235
VneI GTGCAC 1 cut(s) 314
VpaK11BI GGWCC 1 cut(s) 398
XapI RAATTY 3 cut(s) 55, 179, 419
XceI RCATGY 2 cut(s) 8, 640
XmnI GAANNNNTTC 1 cut(s) 294
XspI CTAG 2 cut(s) 174, 383
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.