Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

OG0008435

Physical Location & Seq

Genomic Coordinates Forward (+)

4504610 .. 4519506

Gene Structure 14897 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3423 bp

ATGCACTTCATTTCAACATTTCCTTGGGAATTTACCGACTTTGGTGGTTGCTTAAGAGTGAAATTACAAGAATGGCTTATGGGGTGTGGTAAGAAGAATTGTTATGGCAACTGCTGCAGTACTGAGACGAAGCTTCTGAAGGGTCTCCATGAATTCCCCAGTAGGTTTACAGTTCATTATTCAGTTGATAGGTCCATCACTTTTGATGATGAAGACTTGGAATCCTGGGAATTTGAAGCAAAGCGGTGGGCACGAGTGCTTTTTCTTACATGCCAGGAGGAATATCATTTGATACCTACACTGATGTTTATTCAAACTCATGCTCTTGGTCTCTGCCAAGAAAACAACAATTTGGATCAAATTCCTGTGAAGTTTCTCATTGTAATTTTGAGCTTGGTGCGGGAGCTTCAGATGATGCAAGACAGAGTCGCTGAATATAGATCTACAGTTAGAACCAAATCAGAATCTCGTGCGCTTGAATTAATGGATCAATTTGGTCAGCCAGATGCTCTTAACTTTTACCAGAAGTTTACCAATGTTTTTATTCTTATAATGAAGGAGTTGGTGTCTTTAGCAAACTTGTCCTGTTCTATTTTCTCACATGCCAATACCACTAAGATGGCAGATGCCAGCTTTCCTGGTTCAGTGAAAGGAAAACTTGGAGGCCCTAGTCAACGCCGTTTGTCATCCTCTACCACCTCCGCTGTGTTGCAAGCGATCATTTCCATGAAGGCTCTTGCAACTATCTCATCATGGTGTGCACAATTTATAGCTGATGCATCACTTGATCTTGCTTTTAATTTTATGTGGGAATTCTACTGGAAAACTGTTTCATCTCCAGTTACTGATTCAGAGACTGGGGCGGAAATAAGTCTTGCAGCATATGAAGCATTAGCTCCTGCTCTCACAGCACTAGTGTCTATGTTTTCTCCTAAATCGTTGGATCTTGTCACGAACAATGGCAATCTGTTTTTATCAGATGGAAAACCTCTGTTAGATTCCTTGGTTCTTTCTTTTCTTCAGAACATAAATAATCTCCTAGCAATTGGAGTATTTGTACGGACTCGACGTGCAGTTTTAATGAATTGGAAGTGGATTTGCCTAGAATCTCTACTGTCAATTCCTTGCTATGCTTTAAAAAAAGGGCTTCATTTAGAGGACAATAATTTTTTGCTCTCAGGTGATACTCTCAGATTGATTTTTACTGATCTTCTTGAGAGCCTAGAGAATGCTGGAGAAAATTCTGTTCTACCAATGCTCAGATCAGTCCGATTAGTTTTGGGCCTATTGTCCGAGGGAAAGTCTGGTTCACTTATCTATTCATGTGATGGGGTGGATGCCCAGATGATGTGGCAGTTGGTTCAGTCTTCTTGGATTTTGCATGTCAGTTGTAACAAGAGGAAGGTTGCACCTATTGCTGCACTTTTGTCTTCTGTTCTGCATTCATCCCTCTTTAGTGATGAGAGCATGCATATGAATGACAATGCACCTGGGCCTCTGAAGTGGTTTGTTGAGAAAATTCTGGAGGAAGGCACAAAAAGTCCCCGCACAATTCGTCTTGCTGCATTACACTTAACGGGCCTCTTTCTATCATATCCTAGGATTATAAAGTACTATGTGAAGGAGTTGAAGCTGCTGTCACTACATGGTTCTGTTGCATTCGATGAGGACTTTGAAGGTGAATTAGCTGATAATCATGATACAAGGACTGAAGTTTCTTTATTGGCGAAAGGCCCTGATCCTGAGCTTACCAAAGAATTTATCAACACAGAATTGTATGCACGTGCCTCTGTTGCTGTTCTGTTTTACAAGCTAGCAGACTTGTCCGATATGGTGGGATCACCAAATGAAAATGAAGATTGCCATGCAGCTTTGGAATCTGGAAAAATATTTTTGCTTGAGCTTCTAGATTCTGCGGTAAACGACAAGGATCTTTCGAAAGAGCTGTATAAGAAATACAGTGCAATCCACAGGCGTAAGGTACGTGCATGGCAAATGATATGTATTTTGTCACGTTTCATATGTCAAGATATAGTTTCTGAAGTTTCACGTTGCCTGCACATATGCCTTTATATGAACAATTTACCTGCAGTTCGTCAATACTTGGAAACATTCGCAATTAATATGTACTTGAAGTTCCCATCATTGGTTGCGGAGCAACTAGTCCCTGTATTAAGAGATTATGAGATGAGGCCTCAGGCACTCTCTTCATATGTATTTATAGCAGCGAATGTCATCCTCCATGCATCTCAAGCTGTTCAAGACAAGCATTTGAATGAATTACTTCCTCCTATATTTCCACTACTAACATCCCATCACCACAGTTTGCGTGGTTTCGCCCAGTTATTGGTTTACCAAGTTCTCTGCAAATTGTTTCCTCCATTGGACTCTAGGGCCTCTGGAACTATGACTTTAGAGAAGAGGTGTTTTGAGGATTTGAAATCCTACCTGGCAAAGAACTCTGATTGTGTGCGCCTACGAGCATCAATGGGAGGATTTCTTGATGCCTACAGTCCCAGTAGCTCTGTCACTCCTGCTGGGATTTTCATCAACCGGGTTGAGGCACTCGAGTTTGAATGTGTACCAGTGTCACTTACGGAGCAAGTGCTCAACTTTTTAAATGATGTCAGGGAAGATCTTCGGAGTTCAATGGCAAAAGATGCTGCGGCTATTAAGAATGAAAGCTTAAGATCTGATGAAGATCAAAATTGCACAGCAATACCATCTAATGCAAATGAAGGAAACTCACATACCCAGCAGCCCAAAGATATTTCACTGGATTTTCAAAAAAAGATTACCCTCTCTAAACATGAGAAGCATGACATAGCCGTAAATTCCTTCTTTGGCAACCAAGACACTTACAAACCACTTGAAGAAATGGAGAAGGAAGATAAGCTTCTTGCTCAGGTGCTGCAGTCTAGAAGTCAGGCAATGGAAAGAGAGAGAGCAAGCAGACAACAACTCATTCTTGTGGCATCTCTTCTTGATCGCATACCTAACCTTGCTGGCCTGGCACGGACTTGTGAGGTGTTTAAGGTGTCGGGTTTGGCTGTTGCGGATGCAAACGTAGTACATGACAAACAGTTCCAGCTCATCAGTGTGACTGCAGAAAAGTGGGTTCCTATCATTGAAGTCCCAGTGGATAGTTTGAAGGTCTTCCTTGAGAGAAAGAAACGAGAAGGCTTTTCAATTTTGGGACTAGAGCAAACTGCAAATAGTGTACCGCTTGACCAGTATAGCTTTCCTAAAAAGACGGTACTGGTCCTTGGGCGTGAAAAGGAAGGTATACCGGTGGATATCATTCATATTCTGGATGCTTGCATTGAGATTCCACAGTTGGGAGTTGTTCGATCTTTAAATGTTCATGTCAGCGGTGCCATTGCGCTGTGGGAATATACTCGACAGCAGAGATCATCCCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000154 GO:0000451 GO:0000453 GO:0001510 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006355 GO:0006357 GO:0006364 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0008168 GO:0008171 GO:0008173 GO:0008175 GO:0008649 GO:0008757 GO:0009451 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016072 GO:0016423 GO:0016435 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0022613 GO:0030488 GO:0031167 GO:0031323 GO:0031326 GO:0032259 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070039 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140098 GO:0140101 GO:0140102 GO:1901360 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SpoU_methylase

Protein Analysis

1141

Amino Acids

128.21

Weight (kDa)

5.94

Isoelectric Point (pI)

42.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SpoU_methylase	PF00588	988 - 1130	1.3e-26	SpoU rRNA Methylase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008435)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G17610 AT4G17610 AT4G17610
fragaria_vesca	FvH4_5g20370 FvH4_5g20370 FvH4_5g20370 FvH4_5g20370 FvH4_5g20370
malus_domestica	MD04G1055600.v1.1
prunus_persica	Prupe.5G055400_v2.0.a1 Prupe.5G055400_v2.0.a1
pyrus_communis	pycom04g04890
rosa_chinensis	RchiOBHm_Chr7g0205631
rosa_laevigata	RLG00000003369
rosa_multiflora	Rmu_ssc0000482.1_g000025
rosa_roxburghii	Rroxscaffold_3G00252130
rosa_rugosa	Rorug07G0092300
rosa_samantha	Rh7AG223100 Rh7AG230100 Rh7BG218100 Rh7CG236300 Rh7DG230000
rosa_wichuraiana	Rw7G019240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	551, 1607
Acc36I	ACCTGC	1 cut(s)	2095
AccB1I	GGYRCC	1 cut(s)	3374
AccB7I	CCANNNNNTGG	1 cut(s)	2347
AccI	GTMKAC	1 cut(s)	3286
AclWI	GGATC	6 cut(s)	363, 495, 951, 1733, 1846, 1938
AcsI	RAATTY	9 cut(s)	29, 152, 230, 360, 812, 1240, 1518, 1757, 2833
AcuI	CTGAAG	6 cut(s)	158, 392, 1004, 1520, 1731, 2061
AcvI	CACGTG	1 cut(s)	1784
AfaI	GTAC	9 cut(s)	121, 1059, 1613, 1983, 2129, 2583, 3072, 3222, 3258
AfiI	CCNNNNNNNGG	4 cut(s)	2146, 2347, 2560, 3338
AflII	CTTAAG	2 cut(s)	52, 2686
AgeI	ACCGGT	1 cut(s)	3289
AhlI	ACTAGT	2 cut(s)	913, 2161
AjiI	CACGTC	1 cut(s)	1070
AjnI	CCWGG	6 cut(s)	224, 273, 637, 1489, 2448, 3009
Alw21I	GWGCWC	2 cut(s)	763, 2610
Alw26I	GTCTC	4 cut(s)	119, 149, 335, 848
Alw44I	GTGCAC	1 cut(s)	759
AlwI	GGATC	6 cut(s)	363, 495, 951, 1733, 1846, 1938
AlwNI	CAGNNNCTG	3 cut(s)	845, 857, 2911
Ama87I	CYCGRG	1 cut(s)	2567
AoxI	GGCC	8 cut(s)	664, 1282, 1493, 1579, 1732, 2192, 2394, 3007
ApaLI	GTGCAC	1 cut(s)	759
ApoI	RAATTY	9 cut(s)	29, 152, 230, 360, 812, 1240, 1518, 1757, 2833
AseI	ATTAAT	2 cut(s)	482, 2121
AsiGI	ACCGGT	1 cut(s)	3289
Asp700I	GAANNNNTTC	3 cut(s)	2283, 2637, 3155
AspA2I	CCTAGG	1 cut(s)	1598
AspLEI	GCGC	3 cut(s)	475, 2475, 3385
AspS9I	GGNCC	8 cut(s)	192, 665, 1282, 1493, 1579, 1733, 2394, 3262
AsuC2I	CCSGG	1 cut(s)	2555
AsuHPI	GGTGA	4 cut(s)	1193, 1691, 1833, 2309
AsuII	TTCGAA	1 cut(s)	1937
AsuNHI	GCTAGC	1 cut(s)	1813
AvaI	CYCGRG	1 cut(s)	2567
AvaII	GGWCC	2 cut(s)	192, 3262
AvrII	CCTAGG	1 cut(s)	1598
AxyI	CCTNAGG	1 cut(s)	2196
BaeGI	GKGCMC	2 cut(s)	253, 763
BanI	GGYRCC	1 cut(s)	3374
BauI	CACGAG	2 cut(s)	252, 468
BbrPI	CACGTG	1 cut(s)	1784
BbsI	GAAGAC	4 cut(s)	219, 1359, 1422, 3148
Bbv12I	GWGCWC	2 cut(s)	763, 2610
BccI	CCATC	7 cut(s)	203, 613, 974, 1322, 2149, 2322, 2731
BceAI	ACGGC	2 cut(s)	663, 2813
BciT130I	CCWGG	6 cut(s)	226, 275, 639, 1491, 2450, 3011
BcnI	CCSGG	1 cut(s)	2555
BcoDI	GTCTC	4 cut(s)	119, 149, 335, 848
BcuI	ACTAGT	2 cut(s)	913, 2161
BfmI	CTRYAG	6 cut(s)	115, 444, 2088, 2509, 2912, 3105
BfrI	CTTAAG	2 cut(s)	52, 2686
BfuAI	ACCTGC	1 cut(s)	2095
BglII	AGATCT	3 cut(s)	440, 2635, 2690
BlnI	CCTAGG	1 cut(s)	1598
BmcAI	AGTACT	2 cut(s)	121, 1613
Bme1390I	CCNGG	7 cut(s)	226, 275, 639, 1491, 2450, 2555, 3011
Bme18I	GGWCC	2 cut(s)	192, 3262
BmeT110I	CYCGRG	1 cut(s)	2567
BmgBI	CACGTC	1 cut(s)	1070
BmgT120I	GGNCC	8 cut(s)	192, 665, 1282, 1493, 1579, 1733, 2394, 3262
BmiI	GGNNCC	2 cut(s)	3120, 3376
BmrFI	CCNGG	7 cut(s)	226, 275, 639, 1491, 2450, 2555, 3011
BmrI	ACTGGG	6 cut(s)	153, 867, 2335, 2511, 3131, 3412
BmtI	GCTAGC	1 cut(s)	1817
BmuI	ACTGGG	6 cut(s)	153, 867, 2335, 2511, 3131, 3412
BpiI	GAAGAC	4 cut(s)	219, 1359, 1422, 3148
BpmI	CTGGAG	3 cut(s)	822, 1254, 1544
Bpu10I	CCTNAGC	2 cut(s)	1743, 2904
Bpu14I	TTCGAA	1 cut(s)	1937
BpuEI	CTTGAG	4 cut(s)	1235, 1919, 2235, 3182
BpuMI	CCSGG	1 cut(s)	2555
BsaAI	YACGTR	2 cut(s)	1784, 1985
BsaBI	GATNNNNATC	2 cut(s)	2546, 2700
BsaI	GGTCTC	2 cut(s)	149, 335
BsaJI	CCNNGG	7 cut(s)	23, 225, 1002, 1293, 1490, 1598, 3265
BsaWI	WCCGGW	1 cut(s)	3289
BsaXI	ACNNNNNCTCC	4 cut(s)	551, 581, 1041, 1071
Bsc4I	CCNNNNNNNGG	4 cut(s)	2146, 2347, 2560, 3338
Bse118I	RCCGGY	1 cut(s)	3289
Bse21I	CCTNAGG	1 cut(s)	2196
Bse3DI	GCAATG	2 cut(s)	2937, 3378
Bse8I	GATNNNNATC	2 cut(s)	2546, 2700
BseBI	CCWGG	6 cut(s)	226, 275, 639, 1491, 2450, 3011
BseDI	CCNNGG	7 cut(s)	23, 225, 1002, 1293, 1490, 1598, 3265
BseGI	GGATG	8 cut(s)	686, 1342, 1445, 2235, 2309, 3064, 3319, 3413
BseJI	GATNNNNATC	2 cut(s)	2546, 2700
BseLI	CCNNNNNNNGG	4 cut(s)	2146, 2347, 2560, 3338
BseMI	GCAATG	2 cut(s)	2937, 3378
BseMII	CTCAG	7 cut(s)	114, 1191, 1204, 1273, 1734, 2210, 2918
BseSI	GKGCMC	2 cut(s)	253, 763
BseYI	CCCAGC	2 cut(s)	2537, 2754
BsgI	GTGCAG	3 cut(s)	1092, 1404, 2042
BshFI	GGCC	8 cut(s)	666, 1284, 1495, 1581, 1734, 2194, 2396, 3009
BshNI	GGYRCC	1 cut(s)	3374
BshTI	ACCGGT	1 cut(s)	3289
BsiHKAI	GWGCWC	2 cut(s)	763, 2610
BsiHKCI	CYCGRG	1 cut(s)	2567
BsiSI	CCGG	2 cut(s)	2554, 3290
BslFI	GGGAC	5 cut(s)	1527, 2150, 2499, 3119, 3210
BslI	CCNNNNNNNGG	4 cut(s)	2146, 2347, 2560, 3338
BsmAI	GTCTC	4 cut(s)	119, 149, 335, 848
BsmBI	CGTCTC	1 cut(s)	119
BsmFI	GGGAC	5 cut(s)	1527, 2150, 2499, 3119, 3210
BsmI	GAATGC	3 cut(s)	1234, 1441, 1658
BsnI	GGCC	8 cut(s)	666, 1284, 1495, 1581, 1734, 2194, 2396, 3009
Bso31I	GGTCTC	2 cut(s)	149, 335
BsoBI	CYCGRG	1 cut(s)	2567
Bsp119I	TTCGAA	1 cut(s)	1937
Bsp1286I	GDGCHC	3 cut(s)	253, 763, 2610
BspANI	GGCC	8 cut(s)	666, 1284, 1495, 1581, 1734, 2194, 2396, 3009
BspCNI	CTCAG	7 cut(s)	115, 1190, 1203, 1272, 1735, 2209, 2917
BspHI	TCATGA	1 cut(s)	1696
BspLI	GGNNCC	2 cut(s)	3120, 3376
BspMAI	CTGCAG	4 cut(s)	119, 2092, 2916, 3109
BspMI	ACCTGC	1 cut(s)	2095
BspOI	GCTAGC	1 cut(s)	1817
BspPI	GGATC	6 cut(s)	363, 495, 951, 1733, 1846, 1938
BspT104I	TTCGAA	1 cut(s)	1937
BspT107I	GGYRCC	1 cut(s)	3374
BspTI	CTTAAG	2 cut(s)	52, 2686
BspTNI	GGTCTC	2 cut(s)	149, 335
BsrDI	GCAATG	2 cut(s)	2937, 3378
BsrFI	RCCGGY	1 cut(s)	3289
BssAI	RCCGGY	1 cut(s)	3289
BssECI	CCNNGG	7 cut(s)	23, 225, 1002, 1293, 1490, 1598, 3265
BssNAI	GTATAC	1 cut(s)	3287
BssSI	CACGAG	2 cut(s)	252, 468
BssT1I	CCWWGG	4 cut(s)	23, 1002, 1598, 3265
Bst1107I	GTATAC	1 cut(s)	3287
Bst2BI	CACGAG	2 cut(s)	252, 468
Bst2UI	CCWGG	6 cut(s)	226, 275, 639, 1491, 2450, 3011
Bst6I	CTCTTC	3 cut(s)	2212, 2414, 2985
BstAFI	CTTAAG	2 cut(s)	52, 2686
BstAPI	GCANNNNNTGC	3 cut(s)	114, 1415, 2660
BstBAI	YACGTR	2 cut(s)	1784, 1985
BstBI	TTCGAA	1 cut(s)	1937
BstC8I	GCNNGC	8 cut(s)	631, 714, 1469, 1815, 2057, 2950, 3007, 3319
BstDEI	CTNAG	8 cut(s)	123, 615, 1177, 1190, 1259, 1743, 2196, 2904
BstF5I	GGATG	8 cut(s)	686, 1342, 1445, 2235, 2309, 3064, 3319, 3413
BstHHI	GCGC	3 cut(s)	475, 2475, 3385
BstMAI	GTCTC	4 cut(s)	119, 149, 335, 848
BstMWI	GCNNNNNNNGC	8 cut(s)	114, 887, 908, 1415, 1793, 2252, 2660, 3011
BstNI	CCWGG	6 cut(s)	226, 275, 639, 1491, 2450, 3011
BstNSI	RCATGY	4 cut(s)	273, 605, 1385, 1471
BstSCI	CCNGG	7 cut(s)	224, 273, 637, 1489, 2448, 2553, 3009
BstSFI	CTRYAG	6 cut(s)	115, 444, 2088, 2509, 2912, 3105
BstSLI	GKGCMC	2 cut(s)	253, 763
BstV2I	GAAGAC	4 cut(s)	219, 1359, 1422, 3148
BstX2I	RGATCY	5 cut(s)	440, 943, 1930, 2635, 2690
BstXI	CCANNNNNNTGG	1 cut(s)	619
BstYI	RGATCY	5 cut(s)	440, 943, 1930, 2635, 2690
BstZ17I	GTATAC	1 cut(s)	3287
Bsu36I	CCTNAGG	1 cut(s)	2196
BsuRI	GGCC	8 cut(s)	666, 1284, 1495, 1581, 1734, 2194, 2396, 3009
BtrI	CACGTC	1 cut(s)	1070
BtsCI	GGATG	8 cut(s)	686, 1342, 1445, 2235, 2309, 3064, 3319, 3413
BtsIMutI	CAGTG	7 cut(s)	299, 651, 1966, 2592, 2774, 3103, 3144
BveI	ACCTGC	1 cut(s)	2095
Cac8I	GCNNGC	8 cut(s)	631, 714, 1469, 1815, 2057, 2950, 3007, 3319
CaiI	CAGNNNCTG	3 cut(s)	845, 857, 2911
CciI	TCATGA	1 cut(s)	1696
CfoI	GCGC	3 cut(s)	475, 2475, 3385
Cfr10I	RCCGGY	1 cut(s)	3289
Cfr13I	GGNCC	8 cut(s)	192, 665, 1282, 1493, 1579, 1733, 2394, 3262
Csp6I	GTAC	9 cut(s)	120, 1058, 1612, 1982, 2128, 2582, 3071, 3221, 3257
CspAI	ACCGGT	1 cut(s)	3289
CviQI	GTAC	9 cut(s)	120, 1058, 1612, 1982, 2128, 2582, 3071, 3221, 3257
DdeI	CTNAG	8 cut(s)	123, 615, 1177, 1190, 1259, 1743, 2196, 2904
DraI	TTTAAA	3 cut(s)	1137, 2619, 3357
Eam1104I	CTCTTC	3 cut(s)	2212, 2414, 2985
EarI	CTCTTC	3 cut(s)	2212, 2414, 2985
EciI	GGCGGA	1 cut(s)	878
Eco130I	CCWWGG	4 cut(s)	23, 1002, 1598, 3265
Eco147I	AGGCCT	1 cut(s)	2194
Eco31I	GGTCTC	2 cut(s)	149, 335
Eco32I	GATATC	1 cut(s)	3298
Eco47I	GGWCC	2 cut(s)	192, 3262
Eco57I	CTGAAG	6 cut(s)	158, 392, 1004, 1520, 1731, 2061
Eco72I	CACGTG	1 cut(s)	1784
Eco81I	CCTNAGG	1 cut(s)	2196
Eco88I	CYCGRG	1 cut(s)	2567
EcoO109I	RGGNCCY	3 cut(s)	665, 1733, 2394
EcoRI	GAATTC	2 cut(s)	152, 812
EcoRII	CCWGG	6 cut(s)	224, 273, 637, 1489, 2448, 3009
EcoRV	GATATC	1 cut(s)	3298
EcoT14I	CCWWGG	4 cut(s)	23, 1002, 1598, 3265
EcoT22I	ATGCAT	3 cut(s)	781, 1473, 2248
ErhI	CCWWGG	4 cut(s)	23, 1002, 1598, 3265
Esp3I	CGTCTC	1 cut(s)	119
FalI	AAGNNNNNCTT	6 cut(s)	60, 92, 642, 674, 2880, 2912
FaqI	GGGAC	5 cut(s)	1527, 2150, 2499, 3119, 3210
FauI	CCCGC	2 cut(s)	393, 1553
FauNDI	CATATG	5 cut(s)	883, 1473, 2021, 2063, 2212
FblI	GTMKAC	1 cut(s)	3286
FokI	GGATG	8 cut(s)	673, 1349, 1432, 2222, 2296, 3071, 3326, 3400
GlaI	GCGC	3 cut(s)	474, 2474, 3384
GsaI	CCCAGC	2 cut(s)	2541, 2758
GsuI	CTGGAG	3 cut(s)	822, 1254, 1544
HaeIII	GGCC	8 cut(s)	666, 1284, 1495, 1581, 1734, 2194, 2396, 3009
HapII	CCGG	2 cut(s)	2554, 3290
HhaI	GCGC	3 cut(s)	475, 2475, 3385
Hin6I	GCGC	3 cut(s)	473, 2473, 3383
HinP1I	GCGC	3 cut(s)	473, 2473, 3383
HincII	GTYRAC	1 cut(s)	674
HindII	GTYRAC	1 cut(s)	674
HindIII	AAGCTT	3 cut(s)	131, 2683, 2894
HpaII	CCGG	2 cut(s)	2554, 3290
HphI	GGTGA	4 cut(s)	1193, 1691, 1833, 2309
Hpy99I	CGWCG	1 cut(s)	1071
HpyCH4IV	ACGT	6 cut(s)	1069, 1783, 1984, 2014, 2050, 3066
HpyF10VI	GCNNNNNNNGC	8 cut(s)	114, 887, 908, 1415, 1793, 2252, 2660, 3011
HpyF3I	CTNAG	8 cut(s)	123, 615, 1177, 1190, 1259, 1743, 2196, 2904
HpySE526I	ACGT	6 cut(s)	1069, 1783, 1984, 2014, 2050, 3066
HspAI	GCGC	3 cut(s)	473, 2473, 3383
LmnI	GCTCC	4 cut(s)	403, 901, 2155, 2599
MaeII	ACGT	6 cut(s)	1069, 1783, 1984, 2014, 2050, 3066
MaeIII	GTNAC	8 cut(s)	841, 949, 1391, 1638, 2010, 2527, 2589, 3100
MfeI	CAATTG	1 cut(s)	1044
MflI	RGATCY	5 cut(s)	440, 943, 1930, 2635, 2690
MhlI	GDGCHC	3 cut(s)	253, 763, 2610
MlyI	GAGTC	3 cut(s)	435, 1057, 2381
MmeI	TCCRAC	1 cut(s)	921
Mph1103I	ATGCAT	3 cut(s)	781, 1473, 2248
MroXI	GAANNNNTTC	3 cut(s)	2283, 2637, 3155
MslI	CAYNNNNRTG	8 cut(s)	617, 725, 754, 1472, 1476, 2274, 2969, 3098
MspA1I	CMGCKG	2 cut(s)	704, 3372
MspCI	CTTAAG	2 cut(s)	52, 2686
MspI	CCGG	2 cut(s)	2554, 3290
MspR9I	CCNGG	7 cut(s)	226, 275, 639, 1491, 2450, 2555, 3011
MunI	CAATTG	1 cut(s)	1044
Mva1269I	GAATGC	3 cut(s)	1234, 1441, 1658
MvaI	CCWGG	6 cut(s)	226, 275, 639, 1491, 2450, 3011
MwoI	GCNNNNNNNGC	8 cut(s)	114, 887, 908, 1415, 1793, 2252, 2660, 3011
NciI	CCSGG	1 cut(s)	2555
NdeI	CATATG	5 cut(s)	883, 1473, 2021, 2063, 2212
NheI	GCTAGC	1 cut(s)	1813
NlaIV	GGNNCC	2 cut(s)	3120, 3376
NmuCI	GTSAC	6 cut(s)	949, 1638, 2010, 2527, 2589, 3100
NsiI	ATGCAT	3 cut(s)	781, 1473, 2248
NspI	RCATGY	4 cut(s)	273, 605, 1385, 1471
NspV	TTCGAA	1 cut(s)	1937
PaeI	GCATGC	1 cut(s)	1471
PaeR7I	CTCGAG	1 cut(s)	2567
PagI	TCATGA	1 cut(s)	1696
PceI	AGGCCT	1 cut(s)	2194
PcsI	WCGNNNNNNNCGW	1 cut(s)	1066
PctI	GAATGC	3 cut(s)	1234, 1441, 1658
PdmI	GAANNNNTTC	3 cut(s)	2283, 2637, 3155
PfeI	GAWTC	8 cut(s)	221, 464, 848, 998, 1106, 1877, 1910, 3328
PflFI	GACNNNGTC	1 cut(s)	425
PflMI	CCANNNNNTGG	1 cut(s)	2347
PinAI	ACCGGT	1 cut(s)	3289
PleI	GAGTC	3 cut(s)	434, 1057, 2381
PmaCI	CACGTG	1 cut(s)	1784
PmlI	CACGTG	1 cut(s)	1784
PpsI	GAGTC	3 cut(s)	434, 1057, 2381
Ppu21I	YACGTR	2 cut(s)	1784, 1985
PshBI	ATTAAT	2 cut(s)	482, 2121
PsiI	TTATAA	2 cut(s)	551, 1607
Psp6I	CCWGG	6 cut(s)	224, 273, 637, 1489, 2448, 3009
PspCI	CACGTG	1 cut(s)	1784
PspFI	CCCAGC	2 cut(s)	2537, 2754
PspGI	CCWGG	6 cut(s)	224, 273, 637, 1489, 2448, 3009
PspN4I	GGNNCC	2 cut(s)	3120, 3376
PspPI	GGNCC	8 cut(s)	192, 665, 1282, 1493, 1579, 1733, 2394, 3262
PspXI	VCTCGAGB	1 cut(s)	2567
PsrI	GAACNNNNNNTAC	2 cut(s)	2120, 2152
PstI	CTGCAG	4 cut(s)	119, 2092, 2916, 3109
PstNI	CAGNNNCTG	3 cut(s)	845, 857, 2911
PsuI	RGATCY	5 cut(s)	440, 943, 1930, 2635, 2690
PsyI	GACNNNGTC	1 cut(s)	425
RsaI	GTAC	9 cut(s)	121, 1059, 1613, 1983, 2129, 2583, 3072, 3222, 3258
RsaNI	GTAC	9 cut(s)	120, 1058, 1612, 1982, 2128, 2582, 3071, 3221, 3257
RseI	CAYNNNNRTG	8 cut(s)	617, 725, 754, 1472, 1476, 2274, 2969, 3098
Sau96I	GGNCC	8 cut(s)	192, 665, 1282, 1493, 1579, 1733, 2394, 3262
ScaI	AGTACT	2 cut(s)	121, 1613
SchI	GAGTC	3 cut(s)	435, 1057, 2381
ScrFI	CCNGG	7 cut(s)	226, 275, 639, 1491, 2450, 2555, 3011
SduI	GDGCHC	3 cut(s)	253, 763, 2610
SfcI	CTRYAG	6 cut(s)	115, 444, 2088, 2509, 2912, 3105
Sfr274I	CTCGAG	1 cut(s)	2567
SfuI	TTCGAA	1 cut(s)	1937
SinI	GGWCC	2 cut(s)	192, 3262
SlaI	CTCGAG	1 cut(s)	2567
SmiMI	CAYNNNNRTG	8 cut(s)	617, 725, 754, 1472, 1476, 2274, 2969, 3098
SmlI	CTYRAG	7 cut(s)	52, 1214, 1898, 2250, 2567, 2686, 3161
SmoI	CTYRAG	7 cut(s)	52, 1214, 1898, 2250, 2567, 2686, 3161
SpeI	ACTAGT	2 cut(s)	913, 2161
SphI	GCATGC	1 cut(s)	1471
SseBI	AGGCCT	1 cut(s)	2194
SspI	AATATT	1 cut(s)	1890
StuI	AGGCCT	1 cut(s)	2194
StyD4I	CCNGG	7 cut(s)	224, 273, 637, 1489, 2448, 2553, 3009
StyI	CCWWGG	4 cut(s)	23, 1002, 1598, 3265
TaiI	ACGT	6 cut(s)	1072, 1786, 1987, 2017, 2053, 3069
TaqI	TCGA	6 cut(s)	1066, 1662, 1937, 2568, 3349, 3400
TatI	WGTACW	4 cut(s)	119, 1611, 2127, 3070
TauI	GCSGC	1 cut(s)	2669
TfiI	GAWTC	8 cut(s)	221, 464, 848, 998, 1106, 1877, 1910, 3328
TscAI	CASTG	7 cut(s)	306, 651, 1966, 2592, 2781, 3103, 3144
TseFI	GTSAC	6 cut(s)	949, 1638, 2010, 2527, 2589, 3100
Tsp45I	GTSAC	6 cut(s)	949, 1638, 2010, 2527, 2589, 3100
TspGWI	ACGGA	3 cut(s)	1075, 2612, 3031
TspRI	CASTG	7 cut(s)	306, 651, 1966, 2592, 2781, 3103, 3144
Tth111I	GACNNNGTC	1 cut(s)	425
Van91I	CCANNNNNTGG	1 cut(s)	2347
Vha464I	CTTAAG	2 cut(s)	52, 2686
VneI	GTGCAC	1 cut(s)	759
VpaK11BI	GGWCC	2 cut(s)	192, 3262
VspI	ATTAAT	2 cut(s)	482, 2121
XapI	RAATTY	9 cut(s)	29, 152, 230, 360, 812, 1240, 1518, 1757, 2833
XbaI	TCTAGA	2 cut(s)	1906, 2918
XceI	RCATGY	4 cut(s)	273, 605, 1385, 1471
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2327
XhoI	CTCGAG	1 cut(s)	2567
XmaJI	CCTAGG	1 cut(s)	1598
XmiI	GTMKAC	1 cut(s)	3286
XmnI	GAANNNNTTC	3 cut(s)	2283, 2637, 3155
ZrmI	AGTACT	2 cut(s)	121, 1613
Zsp2I	ATGCAT	3 cut(s)	781, 1473, 2248

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.