pycom05g07710

carboxylase beta chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002822
Physical Location & Seq
Forward (+)
10585486 .. 10586246
761 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 462 bp
ATGCTTGGCATATTGATGAGAAAATCAGTAGCTTCGGCTTCACGGAAATCACTCGCCGCATTGCAAATCGCACAAACAAGTAGGAATTACAGCCTCGGAGTCCTTCCCGATGGCGTCGACCGGAACTCCGAGGCCTTCTCTCGCAACTCCAAGACCATGGACGTCCTGATCTCCGACCTCCAATTCCACATCCAAAAGGTTCTTGGCGGAGGAGGAACTGTAGCTGTGAAGAGAAACAAGAGCAGAAACAAGCTCCTCCCAAGAGAACGCATTGATCGACTGCTCGACCCCGGCGCTTCTTTTCTCGAGCTTTCTCAGCTTGCAGGACATGAATTGTATGAAGAATCCTTACCATCTGCTGGGATCATTACTGGAATAGGTCCGGTCCATGGACGGCTTTGTATGTTTGTAGCTAATCGATCCTACTGTGAAGGGTGGGACTTACTACCCCATCACAGTTAA

Protein Analysis

154

Amino Acids

16.73

Weight (kDa)

9.51

Isoelectric Point (pI)

44.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carboxyl_trans PF01039 79 - 140 6.1e-12 Carboxyl transferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 165
AccB7I CCANNNNNTGG 1 cut(s) 359
AccI GTMKAC 1 cut(s) 117
AciI CCGC 2 cut(s) 57, 207
AclWI GGATC 2 cut(s) 371, 414
AcyI GRCGYC 2 cut(s) 114, 162
AfiI CCNNNNNNNGG 2 cut(s) 359, 389
AluBI AGCT 6 cut(s) 32, 224, 253, 310, 319, 413
AluI AGCT 6 cut(s) 32, 224, 253, 310, 319, 413
AlwI GGATC 2 cut(s) 371, 414
Ama87I CYCGRG 1 cut(s) 305
AoxI GGCC 1 cut(s) 132
AspLEI GCGC 1 cut(s) 296
AspS9I GGNCC 2 cut(s) 380, 385
AsuC2I CCSGG 1 cut(s) 291
AvaI CYCGRG 1 cut(s) 305
AvaII GGWCC 2 cut(s) 380, 385
BarI GAAGNNNNNNTAC 2 cut(s) 333, 365
BccI CCATC 3 cut(s) 104, 361, 459
BceAI ACGGC 1 cut(s) 410
BcnI CCSGG 1 cut(s) 291
BfmI CTRYAG 1 cut(s) 219
BfoI RGCGCY 1 cut(s) 297
BisI GCNGC 1 cut(s) 57
BlsI GCNGC 1 cut(s) 58
Bme1390I CCNGG 1 cut(s) 291
Bme18I GGWCC 2 cut(s) 380, 385
BmeT110I CYCGRG 1 cut(s) 305
BmgT120I GGNCC 2 cut(s) 380, 385
BmrFI CCNGG 1 cut(s) 291
BplI GAGNNNNNCTC 2 cut(s) 122, 154
BpuMI CCSGG 1 cut(s) 291
Bsa29I ATCGAT 1 cut(s) 418
BsaHI GRCGYC 2 cut(s) 114, 162
BsaJI CCNNGG 5 cut(s) 94, 129, 156, 289, 388
BsaWI WCCGGW 2 cut(s) 120, 382
BsaXI ACNNNNNCTCC 4 cut(s) 110, 140, 204, 234
Bsc4I CCNNNNNNNGG 2 cut(s) 359, 389
Bse1I ACTGG 1 cut(s) 376
Bse3DI GCAATG 1 cut(s) 59
BseCI ATCGAT 1 cut(s) 418
BseDI CCNNGG 5 cut(s) 94, 129, 156, 289, 388
BseGI GGATG 1 cut(s) 189
BseLI CCNNNNNNNGG 2 cut(s) 359, 389
BseMI GCAATG 1 cut(s) 59
BseMII CTCAG 1 cut(s) 329
BseNI ACTGG 1 cut(s) 376
BseRI GAGGAG 2 cut(s) 225, 245
BseYI CCCAGC 1 cut(s) 359
Bsh1285I CGRYCG 1 cut(s) 121
BshFI GGCC 1 cut(s) 134
BshVI ATCGAT 1 cut(s) 418
BsiEI CGRYCG 1 cut(s) 121
BsiHKCI CYCGRG 1 cut(s) 305
BsiSI CCGG 3 cut(s) 121, 291, 383
BslFI GGGAC 1 cut(s) 452
BslI CCNNNNNNNGG 2 cut(s) 359, 389
BsmFI GGGAC 1 cut(s) 452
BsnI GGCC 1 cut(s) 134
BsoBI CYCGRG 1 cut(s) 305
Bsp143I GATC 4 cut(s) 168, 274, 363, 419
Bsp19I CCATGG 2 cut(s) 156, 388
BspACI CCGC 2 cut(s) 57, 207
BspANI GGCC 1 cut(s) 134
BspCNI CTCAG 1 cut(s) 328
BspDI ATCGAT 1 cut(s) 418
BspPI GGATC 2 cut(s) 371, 414
BsrDI GCAATG 1 cut(s) 59
BsrI ACTGG 1 cut(s) 376
BssECI CCNNGG 5 cut(s) 94, 129, 156, 289, 388
BssMI GATC 4 cut(s) 168, 274, 363, 419
BssNI GRCGYC 2 cut(s) 114, 162
BssT1I CCWWGG 2 cut(s) 156, 388
Bst4CI ACNGT 3 cut(s) 220, 428, 458
Bst6I CTCTTC 1 cut(s) 224
BstACI GRCGYC 2 cut(s) 114, 162
BstC8I GCNNGC 1 cut(s) 321
BstDEI CTNAG 1 cut(s) 315
BstDSI CCRYGG 2 cut(s) 156, 388
BstF5I GGATG 1 cut(s) 189
BstH2I RGCGCY 1 cut(s) 297
BstHHI GCGC 1 cut(s) 296
BstKTI GATC 4 cut(s) 171, 277, 366, 422
BstMBI GATC 4 cut(s) 168, 274, 363, 419
BstMCI CGRYCG 1 cut(s) 121
BstMWI GCNNNNNNNGC 1 cut(s) 316
BstSCI CCNGG 1 cut(s) 289
BstSFI CTRYAG 1 cut(s) 219
BstXI CCANNNNNNTGG 1 cut(s) 157
Bsu15I ATCGAT 1 cut(s) 418
BsuRI GGCC 1 cut(s) 134
BsuTUI ATCGAT 1 cut(s) 418
BtgI CCRYGG 2 cut(s) 156, 388
BtsCI GGATG 1 cut(s) 189
Cac8I GCNNGC 1 cut(s) 321
CfoI GCGC 1 cut(s) 296
Cfr13I GGNCC 2 cut(s) 380, 385
ClaI ATCGAT 1 cut(s) 418
CseI GACGC 1 cut(s) 103
CviAII CATG 3 cut(s) 157, 329, 389
DdeI CTNAG 1 cut(s) 315
DpnI GATC 4 cut(s) 170, 276, 365, 421
DpnII GATC 4 cut(s) 168, 274, 363, 419
Eam1104I CTCTTC 1 cut(s) 224
EarI CTCTTC 1 cut(s) 224
EciI GGCGGA 1 cut(s) 222
Eco130I CCWWGG 2 cut(s) 156, 388
Eco147I AGGCCT 1 cut(s) 134
Eco47I GGWCC 2 cut(s) 380, 385
Eco88I CYCGRG 1 cut(s) 305
EcoT14I CCWWGG 2 cut(s) 156, 388
ErhI CCWWGG 2 cut(s) 156, 388
FaeI CATG 3 cut(s) 160, 332, 392
FaiI YATR 6 cut(s) 11, 158, 330, 339, 390, 404
FaqI GGGAC 1 cut(s) 452
FatI CATG 3 cut(s) 156, 328, 388
FblI GTMKAC 1 cut(s) 117
Fnu4HI GCNGC 1 cut(s) 57
FokI GGATG 1 cut(s) 176
Fsp4HI GCNGC 1 cut(s) 57
GlaI GCGC 1 cut(s) 295
GluI GCNGC 1 cut(s) 57
GsaI CCCAGC 1 cut(s) 363
HaeII RGCGCY 1 cut(s) 297
HaeIII GGCC 1 cut(s) 134
HapII CCGG 3 cut(s) 121, 291, 383
HgaI GACGC 1 cut(s) 103
HhaI GCGC 1 cut(s) 296
Hin1I GRCGYC 2 cut(s) 114, 162
Hin1II CATG 3 cut(s) 160, 332, 392
Hin6I GCGC 1 cut(s) 294
HinP1I GCGC 1 cut(s) 294
HincII GTYRAC 1 cut(s) 118
HindII GTYRAC 1 cut(s) 118
HinfI GANTC 2 cut(s) 99, 344
HpaII CCGG 3 cut(s) 121, 291, 383
Hpy166II GTNNAC 1 cut(s) 118
Hpy188I TCNGA 3 cut(s) 98, 130, 175
Hpy188III TCNNGA 3 cut(s) 107, 166, 305
Hpy8I GTNNAC 1 cut(s) 118
Hpy99I CGWCG 1 cut(s) 119
HpyAV CCTTC 3 cut(s) 113, 145, 425
HpyCH4III ACNGT 3 cut(s) 220, 428, 458
HpyCH4IV ACGT 1 cut(s) 162
HpyCH4V TGCA 2 cut(s) 64, 323
HpyF10VI GCNNNNNNNGC 1 cut(s) 316
HpyF3I CTNAG 1 cut(s) 315
HpySE526I ACGT 1 cut(s) 162
Hsp92I GRCGYC 2 cut(s) 114, 162
Hsp92II CATG 3 cut(s) 160, 332, 392
HspAI GCGC 1 cut(s) 294
Kzo9I GATC 4 cut(s) 168, 274, 363, 419
LmnI GCTCC 1 cut(s) 258
LpnPI CCDG 7 cut(s) 134, 179, 304, 309, 345, 357, 396
MaeII ACGT 1 cut(s) 162
MalI GATC 4 cut(s) 170, 276, 365, 421
MboI GATC 4 cut(s) 168, 274, 363, 419
MboII GAAGA 2 cut(s) 241, 353
MluCI AATT 3 cut(s) 85, 182, 332
MlyI GAGTC 1 cut(s) 108
MmeI TCCRAC 1 cut(s) 198
MnlI CCTC 6 cut(s) 104, 124, 188, 203, 206, 266
MseI TTAA 1 cut(s) 460
MslI CAYNNNNRTG 1 cut(s) 14
MspI CCGG 3 cut(s) 121, 291, 383
MspR9I CCNGG 1 cut(s) 291
MwoI GCNNNNNNNGC 1 cut(s) 316
NciI CCSGG 1 cut(s) 291
NcoI CCATGG 2 cut(s) 156, 388
NdeII GATC 4 cut(s) 168, 274, 363, 419
NlaIII CATG 3 cut(s) 160, 332, 392
PaeR7I CTCGAG 1 cut(s) 305
PceI AGGCCT 1 cut(s) 134
PcsI WCGNNNNNNNCGW 1 cut(s) 274
PfeI GAWTC 1 cut(s) 344
PflMI CCANNNNNTGG 1 cut(s) 359
PkrI GCNGC 1 cut(s) 58
PleI GAGTC 1 cut(s) 107
PpsI GAGTC 1 cut(s) 107
PspFI CCCAGC 1 cut(s) 359
PspPI GGNCC 2 cut(s) 380, 385
RseI CAYNNNNRTG 1 cut(s) 14
SalI GTCGAC 1 cut(s) 116
SaqAI TTAA 1 cut(s) 460
SatI GCNGC 1 cut(s) 57
Sau3AI GATC 4 cut(s) 168, 274, 363, 419
Sau96I GGNCC 2 cut(s) 380, 385
SchI GAGTC 1 cut(s) 108
ScrFI CCNGG 1 cut(s) 291
SfcI CTRYAG 1 cut(s) 219
Sfr274I CTCGAG 1 cut(s) 305
SinI GGWCC 2 cut(s) 380, 385
SlaI CTCGAG 1 cut(s) 305
SmiMI CAYNNNNRTG 1 cut(s) 14
SmlI CTYRAG 1 cut(s) 305
SmoI CTYRAG 1 cut(s) 305
Sse9I AATT 3 cut(s) 85, 182, 332
SseBI AGGCCT 1 cut(s) 134
SsiI CCGC 2 cut(s) 57, 207
StuI AGGCCT 1 cut(s) 134
StyD4I CCNGG 1 cut(s) 289
StyI CCWWGG 2 cut(s) 156, 388
TaaI ACNGT 3 cut(s) 220, 428, 458
TaiI ACGT 1 cut(s) 165
TaqI TCGA 5 cut(s) 117, 277, 285, 306, 418
TasI AATT 3 cut(s) 85, 182, 332
TauI GCSGC 1 cut(s) 59
TfiI GAWTC 1 cut(s) 344
Tru1I TTAA 1 cut(s) 460
Tru9I TTAA 1 cut(s) 460
TspDTI ATGAA 2 cut(s) 345, 354
TspGWI ACGGA 1 cut(s) 58
Van91I CCANNNNNTGG 1 cut(s) 359
VpaK11BI GGWCC 2 cut(s) 380, 385
XcmI CCANNNNNNNNNTGG 1 cut(s) 200
XhoI CTCGAG 1 cut(s) 305
XmiI GTMKAC 1 cut(s) 117
ZraI GACGTC 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.