pycom05g12090

LON peptidase N-terminal domain and RING finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0008497
Physical Location & Seq
Forward (+)
15567831 .. 15568538
708 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 639 bp
ATGTCATTTTCGACACCCCACTCCCCAAATCTCCTCTCCTCTCTCGTCTCAGACCTCGCTCGAGCTCCCCATCTTCCCCCTCCCCCTCGTCCTCTTCCCCGGCGCCATCCTCCCCCTCCAGATCTTCGAGTTCCGCTCCAGATCTTCGGGGTTATCTACAATGATGCCGTCTCCGGCACCGTCGACGTTGGCTGCGTCGGCGAGGTCGTCAAGCACGAGCGCCTCGTCGATGACCGGTTCTTCCTCATCTGCAAAGGCCAGGAGCGGTTCCGGGTCACTGACCTCGTCCGTACCAAACCTTACCTGGTGGCGGAGGTCCAATGGCTCGAAGACCTGCCGTCGAACGACGGCGAGGAGGACCTGGAGGCGTTGGCCAGCGAGGTCGAGTTGTATATGAAGGATGTGATTCGGTTGTCAAATCGGTTGGGCGGGAAGCCGGAGAAGGATGTGCAGGACCTGCGACGGAACCTGTTTCCGACGCCGTTTTCGTTTTTCGTTGGGAGCACTTTCGAAGGGGCACCGAGAGAGCAGCAGGCGCTGCTGGAACTGGAGGACACGGCGGCGCGGTTGAAGAGAGAGAAGGACCCGTGGACCCGTACAAATTTTTTTTTTTTTCAAACAACCAAAAAAAATAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LON_substr_bdg

Protein Analysis

213

Amino Acids

24.21

Weight (kDa)

6.13

Isoelectric Point (pI)

44.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LON_substr_bdg PF02190 61 - 192 4.3e-18 ATP-dependent protease La (LON) substrate-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 342, 465
AccB1I GGYRCC 3 cut(s) 102, 176, 517
AccBSI CCGCTC 2 cut(s) 136, 265
AccI GTMKAC 1 cut(s) 183
AccII CGCG 1 cut(s) 565
AciI CCGC 6 cut(s) 134, 265, 311, 429, 560, 565
AcoI YGGCCR 1 cut(s) 372
AcsI RAATTY 1 cut(s) 601
AcyI GRCGYC 2 cut(s) 103, 479
AfaI GTAC 2 cut(s) 292, 598
AfiI CCNNNNNNNGG 1 cut(s) 310
AgeI ACCGGT 1 cut(s) 234
AgsI TTSAA 2 cut(s) 571, 617
AhdI GACNNNNNGTC 1 cut(s) 337
AjnI CCWGG 3 cut(s) 258, 303, 360
AluBI AGCT 1 cut(s) 65
AluI AGCT 1 cut(s) 65
Alw21I GWGCWC 2 cut(s) 67, 506
Alw26I GTCTC 2 cut(s) 52, 175
AlwNI CAGNNNCTG 2 cut(s) 457, 538
Ama87I CYCGRG 1 cut(s) 60
AoxI GGCC 2 cut(s) 256, 372
ApeKI GCWGC 3 cut(s) 192, 529, 538
ApoI RAATTY 1 cut(s) 601
ArsI GACNNNNNNTTYG 2 cut(s) 469, 501
AsiGI ACCGGT 1 cut(s) 234
AspLEI GCGC 4 cut(s) 105, 222, 538, 565
AspS9I GGNCC 5 cut(s) 316, 358, 454, 583, 591
AsuC2I CCSGG 2 cut(s) 100, 272
AsuII TTCGAA 1 cut(s) 510
AvaI CYCGRG 1 cut(s) 60
AvaII GGWCC 5 cut(s) 316, 358, 454, 583, 591
BaeGI GKGCMC 1 cut(s) 520
BalI TGGCCA 1 cut(s) 374
BanI GGYRCC 3 cut(s) 102, 176, 517
BanII GRGCYC 1 cut(s) 67
BauI CACGAG 1 cut(s) 215
BbsI GAAGAC 1 cut(s) 336
Bbv12I GWGCWC 2 cut(s) 67, 506
BbvI GCAGC 3 cut(s) 179, 525, 541
BccI CCATC 2 cut(s) 78, 114
BceAI ACGGC 5 cut(s) 152, 322, 364, 466, 573
BciT130I CCWGG 3 cut(s) 260, 305, 362
BcnI CCSGG 2 cut(s) 100, 272
BcoDI GTCTC 2 cut(s) 52, 175
BfoI RGCGCY 3 cut(s) 106, 223, 539
BfuAI ACCTGC 2 cut(s) 342, 465
BglII AGATCT 2 cut(s) 121, 141
BisI GCNGC 4 cut(s) 193, 530, 539, 561
BlsI GCNGC 4 cut(s) 194, 531, 540, 562
Bme1390I CCNGG 5 cut(s) 100, 260, 272, 305, 362
Bme18I GGWCC 5 cut(s) 316, 358, 454, 583, 591
BmeRI GACNNNNNGTC 1 cut(s) 337
BmeT110I CYCGRG 1 cut(s) 60
BmgT120I GGNCC 5 cut(s) 316, 358, 454, 583, 591
BmiI GGNNCC 7 cut(s) 104, 178, 269, 467, 519, 585, 593
BmrFI CCNGG 5 cut(s) 100, 260, 272, 305, 362
BmsI GCATC 1 cut(s) 154
BpiI GAAGAC 1 cut(s) 336
BplI GAGNNNNNCTC 2 cut(s) 120, 152
BpmI CTGGAG 4 cut(s) 102, 122, 383, 569
Bpu14I TTCGAA 1 cut(s) 510
BpuMI CCSGG 2 cut(s) 100, 272
BsaHI GRCGYC 2 cut(s) 103, 479
BsaJI CCNNGG 2 cut(s) 98, 587
BsaWI WCCGGW 1 cut(s) 234
Bsc4I CCNNNNNNNGG 1 cut(s) 310
Bse118I RCCGGY 1 cut(s) 234
Bse1I ACTGG 1 cut(s) 552
BseBI CCWGG 3 cut(s) 260, 305, 362
BseDI CCNNGG 2 cut(s) 98, 587
BseGI GGATG 3 cut(s) 106, 406, 451
BseLI CCNNNNNNNGG 1 cut(s) 310
BseMII CTCAG 1 cut(s) 63
BseNI ACTGG 1 cut(s) 552
BseRI GAGGAG 3 cut(s) 23, 28, 368
BseSI GKGCMC 1 cut(s) 520
BseXI GCAGC 3 cut(s) 179, 525, 541
BsgI GTGCAG 1 cut(s) 470
Bsh1236I CGCG 1 cut(s) 565
BshFI GGCC 2 cut(s) 258, 374
BshNI GGYRCC 3 cut(s) 102, 176, 517
BshTI ACCGGT 1 cut(s) 234
BsiHKAI GWGCWC 2 cut(s) 67, 506
BsiHKCI CYCGRG 1 cut(s) 60
BsiSI CCGG 5 cut(s) 100, 174, 235, 271, 437
BslI CCNNNNNNNGG 1 cut(s) 310
BsmAI GTCTC 2 cut(s) 52, 175
BsmBI CGTCTC 2 cut(s) 52, 175
BsnI GGCC 2 cut(s) 258, 374
BsoBI CYCGRG 1 cut(s) 60
Bsp119I TTCGAA 1 cut(s) 510
Bsp1286I GDGCHC 3 cut(s) 67, 506, 520
Bsp143I GATC 2 cut(s) 121, 141
BspACI CCGC 6 cut(s) 134, 265, 311, 429, 560, 565
BspANI GGCC 2 cut(s) 258, 374
BspCNI CTCAG 1 cut(s) 62
BspFNI CGCG 1 cut(s) 565
BspLI GGNNCC 7 cut(s) 104, 178, 269, 467, 519, 585, 593
BspMI ACCTGC 2 cut(s) 342, 465
BspT104I TTCGAA 1 cut(s) 510
BspT107I GGYRCC 3 cut(s) 102, 176, 517
BsrBI CCGCTC 2 cut(s) 136, 265
BsrFI RCCGGY 1 cut(s) 234
BsrI ACTGG 1 cut(s) 552
BssAI RCCGGY 1 cut(s) 234
BssECI CCNNGG 2 cut(s) 98, 587
BssMI GATC 2 cut(s) 121, 141
BssNI GRCGYC 2 cut(s) 103, 479
BssSI CACGAG 1 cut(s) 215
Bst2BI CACGAG 1 cut(s) 215
Bst2UI CCWGG 3 cut(s) 260, 305, 362
Bst4CI ACNGT 1 cut(s) 181
Bst6I CTCTTC 2 cut(s) 99, 566
BstACI GRCGYC 2 cut(s) 103, 479
BstAPI GCANNNNNTGC 2 cut(s) 457, 538
BstBI TTCGAA 1 cut(s) 510
BstC8I GCNNGC 2 cut(s) 376, 534
BstDEI CTNAG 1 cut(s) 49
BstDSI CCRYGG 1 cut(s) 587
BstF5I GGATG 3 cut(s) 106, 406, 451
BstFNI CGCG 1 cut(s) 565
BstH2I RGCGCY 3 cut(s) 106, 223, 539
BstHHI GCGC 4 cut(s) 105, 222, 538, 565
BstKTI GATC 2 cut(s) 124, 144
BstMAI GTCTC 2 cut(s) 52, 175
BstMBI GATC 2 cut(s) 121, 141
BstMWI GCNNNNNNNGC 4 cut(s) 198, 457, 535, 538
BstNI CCWGG 3 cut(s) 260, 305, 362
BstSCI CCNGG 5 cut(s) 98, 258, 270, 303, 360
BstSLI GKGCMC 1 cut(s) 520
BstUI CGCG 1 cut(s) 565
BstV1I GCAGC 3 cut(s) 179, 525, 541
BstV2I GAAGAC 1 cut(s) 336
BstX2I RGATCY 2 cut(s) 121, 141
BstYI RGATCY 2 cut(s) 121, 141
BsuRI GGCC 2 cut(s) 258, 374
BtgI CCRYGG 1 cut(s) 587
BtsCI GGATG 3 cut(s) 106, 406, 451
BtsIMutI CAGTG 1 cut(s) 276
BveI ACCTGC 2 cut(s) 342, 465
Cac8I GCNNGC 2 cut(s) 376, 534
CaiI CAGNNNCTG 2 cut(s) 457, 538
CfoI GCGC 4 cut(s) 105, 222, 538, 565
Cfr10I RCCGGY 1 cut(s) 234
Cfr13I GGNCC 5 cut(s) 316, 358, 454, 583, 591
CseI GACGC 2 cut(s) 184, 487
CsiI ACCWGGT 1 cut(s) 303
Csp6I GTAC 2 cut(s) 291, 597
CspAI ACCGGT 1 cut(s) 234
CviJI RGCY 6 cut(s) 65, 192, 258, 325, 374, 436
CviKI_1 RGCY 6 cut(s) 65, 192, 258, 325, 374, 436
CviQI GTAC 2 cut(s) 291, 597
DdeI CTNAG 1 cut(s) 49
DinI GGCGCC 1 cut(s) 104
DpnI GATC 2 cut(s) 123, 143
DpnII GATC 2 cut(s) 121, 141
DriI GACNNNNNGTC 1 cut(s) 337
EaeI YGGCCR 1 cut(s) 372
Eam1104I CTCTTC 2 cut(s) 99, 566
Eam1105I GACNNNNNGTC 1 cut(s) 337
EarI CTCTTC 2 cut(s) 99, 566
EciI GGCGGA 1 cut(s) 326
Ecl136II GAGCTC 1 cut(s) 65
Eco24I GRGCYC 1 cut(s) 67
Eco47I GGWCC 5 cut(s) 316, 358, 454, 583, 591
Eco53kI GAGCTC 1 cut(s) 65
Eco88I CYCGRG 1 cut(s) 60
EcoICRI GAGCTC 1 cut(s) 65
EcoO109I RGGNCCY 3 cut(s) 358, 454, 583
EcoRII CCWGG 3 cut(s) 258, 303, 360
EcoT38I GRGCYC 1 cut(s) 67
EgeI GGCGCC 1 cut(s) 104
EheI GGCGCC 1 cut(s) 104
Esp3I CGTCTC 2 cut(s) 52, 175
FaiI YATR 2 cut(s) 393, 395
FauI CCCGC 1 cut(s) 422
FblI GTMKAC 1 cut(s) 183
Fnu4HI GCNGC 4 cut(s) 193, 530, 539, 561
FokI GGATG 3 cut(s) 93, 413, 458
FriOI GRGCYC 1 cut(s) 67
Fsp4HI GCNGC 4 cut(s) 193, 530, 539, 561
GlaI GCGC 4 cut(s) 104, 221, 537, 564
GluI GCNGC 4 cut(s) 193, 530, 539, 561
GsuI CTGGAG 4 cut(s) 102, 122, 383, 569
HaeII RGCGCY 3 cut(s) 106, 223, 539
HaeIII GGCC 2 cut(s) 258, 374
HapII CCGG 5 cut(s) 100, 174, 235, 271, 437
HgaI GACGC 2 cut(s) 184, 487
HhaI GCGC 4 cut(s) 105, 222, 538, 565
Hin1I GRCGYC 2 cut(s) 103, 479
Hin6I GCGC 4 cut(s) 103, 220, 536, 563
HinP1I GCGC 4 cut(s) 103, 220, 536, 563
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
HinfI GANTC 1 cut(s) 406
HpaII CCGG 5 cut(s) 100, 174, 235, 271, 437
Hpy166II GTNNAC 2 cut(s) 184, 591
Hpy188I TCNGA 2 cut(s) 52, 477
Hpy188III TCNNGA 2 cut(s) 119, 139
Hpy8I GTNNAC 2 cut(s) 184, 591
Hpy99I CGWCG 8 cut(s) 185, 188, 200, 230, 343, 350, 465, 481
HpyAV CCTTC 4 cut(s) 391, 436, 506, 574
HpyCH4III ACNGT 1 cut(s) 181
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 2 cut(s) 252, 451
HpyF10VI GCNNNNNNNGC 4 cut(s) 198, 457, 535, 538
HpyF3I CTNAG 1 cut(s) 49
HpySE526I ACGT 1 cut(s) 186
Hsp92I GRCGYC 2 cut(s) 103, 479
HspAI GCGC 4 cut(s) 103, 220, 536, 563
KasI GGCGCC 1 cut(s) 102
Kzo9I GATC 2 cut(s) 121, 141
LmnI GCTCC 4 cut(s) 70, 141, 262, 501
Lsp1109I GCAGC 3 cut(s) 179, 525, 541
LweI GCATC 1 cut(s) 154
MabI ACCWGGT 1 cut(s) 303
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 1 cut(s) 274
MalI GATC 2 cut(s) 123, 143
MbiI CCGCTC 2 cut(s) 136, 265
MboI GATC 2 cut(s) 121, 141
MboII GAAGA 7 cut(s) 65, 86, 116, 136, 232, 341, 583
MflI RGATCY 2 cut(s) 121, 141
MhlI GDGCHC 3 cut(s) 67, 506, 520
MlsI TGGCCA 1 cut(s) 374
MluCI AATT 1 cut(s) 601
MluNI TGGCCA 1 cut(s) 374
Mly113I GGCGCC 1 cut(s) 103
MmeI TCCRAC 1 cut(s) 500
Mox20I TGGCCA 1 cut(s) 374
MscI TGGCCA 1 cut(s) 374
Msp20I TGGCCA 1 cut(s) 374
MspI CCGG 5 cut(s) 100, 174, 235, 271, 437
MspR9I CCNGG 5 cut(s) 100, 260, 272, 305, 362
MvaI CCWGG 3 cut(s) 260, 305, 362
MvnI CGCG 1 cut(s) 565
MwoI GCNNNNNNNGC 4 cut(s) 198, 457, 535, 538
NarI GGCGCC 1 cut(s) 103
NciI CCSGG 2 cut(s) 100, 272
NdeII GATC 2 cut(s) 121, 141
NlaIV GGNNCC 7 cut(s) 104, 178, 269, 467, 519, 585, 593
NmuCI GTSAC 1 cut(s) 274
NspV TTCGAA 1 cut(s) 510
PaeR7I CTCGAG 1 cut(s) 60
PcsI WCGNNNNNNNCGW 5 cut(s) 192, 204, 213, 222, 485
PfeI GAWTC 1 cut(s) 406
PflFI GACNNNGTC 1 cut(s) 284
PinAI ACCGGT 1 cut(s) 234
PkrI GCNGC 4 cut(s) 194, 531, 540, 562
PluTI GGCGCC 1 cut(s) 106
PpuMI RGGWCCY 3 cut(s) 358, 454, 583
Psp124BI GAGCTC 1 cut(s) 67
Psp5II RGGWCCY 3 cut(s) 358, 454, 583
Psp6I CCWGG 3 cut(s) 258, 303, 360
PspGI CCWGG 3 cut(s) 258, 303, 360
PspN4I GGNNCC 7 cut(s) 104, 178, 269, 467, 519, 585, 593
PspPI GGNCC 5 cut(s) 316, 358, 454, 583, 591
PspPPI RGGWCCY 3 cut(s) 358, 454, 583
PspXI VCTCGAGB 1 cut(s) 60
PstNI CAGNNNCTG 2 cut(s) 457, 538
PsuI RGATCY 2 cut(s) 121, 141
PsyI GACNNNGTC 1 cut(s) 284
RsaI GTAC 2 cut(s) 292, 598
RsaNI GTAC 2 cut(s) 291, 597
SacI GAGCTC 1 cut(s) 67
SalI GTCGAC 1 cut(s) 182
SatI GCNGC 4 cut(s) 193, 530, 539, 561
Sau3AI GATC 2 cut(s) 121, 141
Sau96I GGNCC 5 cut(s) 316, 358, 454, 583, 591
ScrFI CCNGG 5 cut(s) 100, 260, 272, 305, 362
SduI GDGCHC 3 cut(s) 67, 506, 520
SexAI ACCWGGT 1 cut(s) 303
SfaNI GCATC 1 cut(s) 154
SfoI GGCGCC 1 cut(s) 104
Sfr274I CTCGAG 1 cut(s) 60
SfuI TTCGAA 1 cut(s) 510
SgrDI CGTCGACG 1 cut(s) 182
SinI GGWCC 5 cut(s) 316, 358, 454, 583, 591
SlaI CTCGAG 1 cut(s) 60
SmlI CTYRAG 1 cut(s) 60
SmoI CTYRAG 1 cut(s) 60
Sse9I AATT 1 cut(s) 601
SsiI CCGC 6 cut(s) 134, 265, 311, 429, 560, 565
SspDI GGCGCC 1 cut(s) 102
SstI GAGCTC 1 cut(s) 67
StyD4I CCNGG 5 cut(s) 98, 258, 270, 303, 360
TaaI ACNGT 1 cut(s) 181
TaiI ACGT 1 cut(s) 189
TaqI TCGA 9 cut(s) 11, 61, 127, 183, 228, 327, 341, 384, 510
TasI AATT 1 cut(s) 601
TauI GCSGC 1 cut(s) 563
TfiI GAWTC 1 cut(s) 406
TscAI CASTG 1 cut(s) 283
TseFI GTSAC 1 cut(s) 274
TseI GCWGC 3 cut(s) 192, 529, 538
Tsp45I GTSAC 1 cut(s) 274
TspDTI ATGAA 1 cut(s) 410
TspGWI ACGGA 2 cut(s) 278, 478
TspRI CASTG 1 cut(s) 283
Tth111I GACNNNGTC 1 cut(s) 284
VpaK11BI GGWCC 5 cut(s) 316, 358, 454, 583, 591
XapI RAATTY 1 cut(s) 601
XcmI CCANNNNNNNNNTGG 1 cut(s) 301
XhoI CTCGAG 1 cut(s) 60
XmiI GTMKAC 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.