pycom05g14050

Tetraketide alpha-pyrone reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0008638
Physical Location & Seq
Reverse (-)
17553205 .. 17554791
1587 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 789 bp
ATGGAAGAAGGCAGCTTTGATGATGCAATTTTTGGGTGCCATGGCGTTTTCCACTCTGCTTCCCCTGTTTTAAAACCTTCGTCTGATCCCAAGACAGAAATCCTGGAACCGCCTGCTGAGGGCACATTGAACATGTTGCGTTCGTGGAAGAAAAATCCGTCTCTAAGGCGCGTGGTTCTCACCTCATCTTCTGCAGCTGTAAAGGTGAGACCAGATGGGGATTTCACCTCCAATGTACCACTGGATGAGTCATCTTGGAGCTTGGTCGAACTCTGGGTGACTCTTCAGATTTGGTACCCTATGTTGAAAATTTTAGCTAAGAAAGCAGGATGGGATTTCTGCAAAGAAAATGGGATTGATATGGTTACTGTCCTACCCACCTTCGTGATCGGGCCTTGTTTGCCACCTGAGTTGTGTTCTACTGCATCAGATGTTCTTGGCCTGCTTAGAGGGGAAACAGAGAAATTTAAGTGGCATGGAAGGATGGGGTACGTTCACATTGATGATGTTGCACTCTGTCACATCTTAGTTTATGAGCATGAAAGTGCTCATGGCAAATATGTTTGTAGCTCAACAGTTATGGACAACACTGAGTTGGGTTCCTTATTATCTACAAGATATCCTTCCTTGCCTATCCCCACAAGGTTTGAGCAACTAGGGAGGCCACATTATTATGAGTTGAACACCTCAAAGATGGAGAGTCTAGGGTTCATGTTCAAGAGTATCCAAGAGATGTTTGATGATTGCATTGCCTCTTTGGTGGAGCAAGGCCATCTTCCTGCATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase UFD1

Protein Analysis

263

Amino Acids

29.27

Weight (kDa)

5.36

Isoelectric Point (pI)

48.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 12 - 183 3.2e-07 NAD dependent epimerase/dehydratase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 294
AccB1I GGYRCC 2 cut(s) 36, 294
AccII CGCG 1 cut(s) 171
AciI CCGC 1 cut(s) 110
AclWI GGATC 1 cut(s) 80
AcsI RAATTY 2 cut(s) 309, 464
AcuI CTGAAG 1 cut(s) 269
AfaI GTAC 3 cut(s) 237, 296, 491
AfiI CCNNNNNNNGG 1 cut(s) 119
AflIII ACRYGT 1 cut(s) 132
AgsI TTSAA 4 cut(s) 130, 307, 682, 718
AjnI CCWGG 1 cut(s) 102
AleI CACNNNNGTG 1 cut(s) 383
AluBI AGCT 5 cut(s) 15, 197, 261, 317, 570
AluI AGCT 5 cut(s) 15, 197, 261, 317, 570
Alw21I GWGCWC 1 cut(s) 550
Alw26I GTCTC 2 cut(s) 165, 202
AlwI GGATC 1 cut(s) 80
AoxI GGCC 4 cut(s) 392, 439, 662, 769
ApeKI GCWGC 2 cut(s) 12, 194
ApoI RAATTY 2 cut(s) 309, 464
Asp718I GGTACC 1 cut(s) 294
AspLEI GCGC 1 cut(s) 171
AspS9I GGNCC 1 cut(s) 392
AsuHPI GGTGA 4 cut(s) 172, 217, 217, 289
BaeGI GKGCMC 1 cut(s) 125
BanI GGYRCC 2 cut(s) 36, 294
Bbv12I GWGCWC 1 cut(s) 550
BbvCI CCTCAGC 1 cut(s) 117
BbvI GCAGC 2 cut(s) 24, 206
BccI CCATC 5 cut(s) 209, 324, 478, 688, 780
BciT130I CCWGG 1 cut(s) 104
BciVI GTATCC 1 cut(s) 734
BcoDI GTCTC 2 cut(s) 165, 202
BfaI CTAG 2 cut(s) 656, 704
BfmI CTRYAG 1 cut(s) 192
BfuI GTATCC 1 cut(s) 734
BisI GCNGC 2 cut(s) 13, 195
BlsI GCNGC 2 cut(s) 14, 196
Bme1390I CCNGG 1 cut(s) 104
BmgT120I GGNCC 1 cut(s) 392
BmiI GGNNCC 4 cut(s) 38, 108, 296, 601
BmrFI CCNGG 1 cut(s) 104
BmsI GCATC 2 cut(s) 13, 434
Bpu10I CCTNAGC 1 cut(s) 117
BsaI GGTCTC 1 cut(s) 202
BsaJI CCNNGG 1 cut(s) 40
Bsc4I CCNNNNNNNGG 1 cut(s) 119
Bse1I ACTGG 1 cut(s) 246
Bse3DI GCAATG 1 cut(s) 747
BseBI CCWGG 1 cut(s) 104
BseDI CCNNGG 1 cut(s) 40
BseGI GGATG 3 cut(s) 250, 335, 489
BseLI CCNNNNNNNGG 1 cut(s) 119
BseMI GCAATG 1 cut(s) 747
BseMII CTCAG 3 cut(s) 108, 399, 582
BseNI ACTGG 1 cut(s) 246
BseSI GKGCMC 1 cut(s) 125
BseXI GCAGC 2 cut(s) 24, 206
Bsh1236I CGCG 1 cut(s) 171
BshFI GGCC 4 cut(s) 394, 441, 664, 771
BshNI GGYRCC 2 cut(s) 36, 294
BsiHKAI GWGCWC 1 cut(s) 550
BslI CCNNNNNNNGG 1 cut(s) 119
BsmAI GTCTC 2 cut(s) 165, 202
BsmBI CGTCTC 1 cut(s) 165
BsnI GGCC 4 cut(s) 394, 441, 664, 771
Bso31I GGTCTC 1 cut(s) 202
Bsp1286I GDGCHC 2 cut(s) 125, 550
Bsp143I GATC 2 cut(s) 85, 387
Bsp19I CCATGG 1 cut(s) 40
BspACI CCGC 1 cut(s) 110
BspANI GGCC 4 cut(s) 394, 441, 664, 771
BspCNI CTCAG 3 cut(s) 109, 400, 583
BspFNI CGCG 1 cut(s) 171
BspLI GGNNCC 4 cut(s) 38, 108, 296, 601
BspMAI CTGCAG 1 cut(s) 196
BspPI GGATC 1 cut(s) 80
BspT107I GGYRCC 2 cut(s) 36, 294
BspTNI GGTCTC 1 cut(s) 202
BsrDI GCAATG 1 cut(s) 747
BsrI ACTGG 1 cut(s) 246
BssECI CCNNGG 1 cut(s) 40
BssMI GATC 2 cut(s) 85, 387
BssT1I CCWWGG 1 cut(s) 40
Bst2UI CCWGG 1 cut(s) 104
Bst4CI ACNGT 2 cut(s) 370, 577
Bst6I CTCTTC 1 cut(s) 288
BstC8I GCNNGC 2 cut(s) 114, 443
BstDEI CTNAG 7 cut(s) 117, 164, 318, 408, 446, 526, 591
BstDSI CCRYGG 1 cut(s) 40
BstF5I GGATG 3 cut(s) 250, 335, 489
BstFNI CGCG 1 cut(s) 171
BstHHI GCGC 1 cut(s) 171
BstKTI GATC 2 cut(s) 88, 390
BstMAI GTCTC 2 cut(s) 165, 202
BstMBI GATC 2 cut(s) 85, 387
BstMWI GCNNNNNNNGC 2 cut(s) 323, 400
BstNI CCWGG 1 cut(s) 104
BstNSI RCATGY 1 cut(s) 136
BstSCI CCNGG 1 cut(s) 102
BstSFI CTRYAG 1 cut(s) 192
BstSLI GKGCMC 1 cut(s) 125
BstUI CGCG 1 cut(s) 171
BstV1I GCAGC 2 cut(s) 24, 206
BsuI GTATCC 1 cut(s) 734
BsuRI GGCC 4 cut(s) 394, 441, 664, 771
BtgI CCRYGG 1 cut(s) 40
BtsCI GGATG 3 cut(s) 250, 335, 489
BtsIMutI CAGTG 2 cut(s) 239, 588
Cac8I GCNNGC 2 cut(s) 114, 443
CfoI GCGC 1 cut(s) 171
Cfr13I GGNCC 1 cut(s) 392
Csp6I GTAC 3 cut(s) 236, 295, 490
CspCI CAANNNNNGTGG 4 cut(s) 393, 428, 628, 663
CviAII CATG 6 cut(s) 41, 133, 476, 539, 551, 712
CviJI RGCY 9 cut(s) 15, 197, 261, 317, 394, 441, 570, 664, 771
CviKI_1 RGCY 9 cut(s) 15, 197, 261, 317, 394, 441, 570, 664, 771
CviQI GTAC 3 cut(s) 236, 295, 490
DdeI CTNAG 7 cut(s) 117, 164, 318, 408, 446, 526, 591
DpnI GATC 2 cut(s) 87, 389
DpnII GATC 2 cut(s) 85, 387
DraI TTTAAA 1 cut(s) 72
Eam1104I CTCTTC 1 cut(s) 288
EarI CTCTTC 1 cut(s) 288
Eco130I CCWWGG 1 cut(s) 40
Eco31I GGTCTC 1 cut(s) 202
Eco32I GATATC 1 cut(s) 620
Eco57I CTGAAG 1 cut(s) 269
EcoRII CCWGG 1 cut(s) 102
EcoRV GATATC 1 cut(s) 620
EcoT14I CCWWGG 1 cut(s) 40
ErhI CCWWGG 1 cut(s) 40
Esp3I CGTCTC 1 cut(s) 165
FaeI CATG 6 cut(s) 44, 136, 479, 542, 554, 715
FalI AAGNNNNNCTT 3 cut(s) 607, 639, 759
FatI CATG 6 cut(s) 40, 132, 475, 538, 550, 711
Fnu4HI GCNGC 2 cut(s) 13, 195
FokI GGATG 3 cut(s) 257, 342, 496
Fsp4HI GCNGC 2 cut(s) 13, 195
FspBI CTAG 2 cut(s) 656, 704
GlaI GCGC 1 cut(s) 170
GluI GCNGC 2 cut(s) 13, 195
HaeIII GGCC 4 cut(s) 394, 441, 664, 771
HhaI GCGC 1 cut(s) 171
Hin1II CATG 6 cut(s) 44, 136, 479, 542, 554, 715
Hin6I GCGC 1 cut(s) 169
HinP1I GCGC 1 cut(s) 169
HinfI GANTC 3 cut(s) 248, 280, 700
HphI GGTGA 4 cut(s) 172, 217, 217, 289
Hpy166II GTNNAC 1 cut(s) 496
Hpy188I TCNGA 3 cut(s) 85, 288, 430
Hpy188III TCNNGA 2 cut(s) 385, 718
Hpy8I GTNNAC 1 cut(s) 496
HpyAV CCTTC 4 cut(s) 87, 391, 474, 633
HpyCH4III ACNGT 2 cut(s) 370, 577
HpyCH4IV ACGT 1 cut(s) 492
HpyCH4V TGCA 7 cut(s) 26, 194, 342, 425, 512, 747, 782
HpyF10VI GCNNNNNNNGC 2 cut(s) 323, 400
HpyF3I CTNAG 7 cut(s) 117, 164, 318, 408, 446, 526, 591
HpySE526I ACGT 1 cut(s) 492
Hsp92II CATG 6 cut(s) 44, 136, 479, 542, 554, 715
HspAI GCGC 1 cut(s) 169
KpnI GGTACC 1 cut(s) 298
Kzo9I GATC 2 cut(s) 85, 387
LmnI GCTCC 2 cut(s) 258, 763
Lsp1109I GCAGC 2 cut(s) 24, 206
LweI GCATC 2 cut(s) 13, 434
MaeI CTAG 2 cut(s) 656, 704
MaeII ACGT 1 cut(s) 492
MaeIII GTNAC 3 cut(s) 277, 364, 518
MalI GATC 2 cut(s) 87, 389
MboI GATC 2 cut(s) 85, 387
MboII GAAGA 5 cut(s) 17, 160, 180, 275, 767
MhlI GDGCHC 2 cut(s) 125, 550
MluCI AATT 3 cut(s) 27, 309, 464
MlyI GAGTC 3 cut(s) 257, 274, 709
MnlI CCTC 7 cut(s) 112, 193, 238, 443, 654, 697, 763
MseI TTAA 2 cut(s) 71, 468
MslI CAYNNNNRTG 4 cut(s) 383, 501, 543, 672
MspA1I CMGCKG 1 cut(s) 197
MspR9I CCNGG 1 cut(s) 104
MvaI CCWGG 1 cut(s) 104
MvnI CGCG 1 cut(s) 171
MwoI GCNNNNNNNGC 2 cut(s) 323, 400
NcoI CCATGG 1 cut(s) 40
NdeII GATC 2 cut(s) 85, 387
NlaIII CATG 6 cut(s) 44, 136, 479, 542, 554, 715
NlaIV GGNNCC 4 cut(s) 38, 108, 296, 601
NmuCI GTSAC 2 cut(s) 277, 518
NspI RCATGY 1 cut(s) 136
OliI CACNNNNGTG 1 cut(s) 383
PciI ACATGT 1 cut(s) 132
PfoI TCCNGGA 1 cut(s) 102
PkrI GCNGC 2 cut(s) 14, 196
PleI GAGTC 3 cut(s) 256, 274, 708
PpsI GAGTC 3 cut(s) 256, 274, 708
PscI ACATGT 1 cut(s) 132
Psp6I CCWGG 1 cut(s) 102
PspGI CCWGG 1 cut(s) 102
PspN4I GGNNCC 4 cut(s) 38, 108, 296, 601
PspPI GGNCC 1 cut(s) 392
PstI CTGCAG 1 cut(s) 196
PvuII CAGCTG 1 cut(s) 197
RsaI GTAC 3 cut(s) 237, 296, 491
RsaNI GTAC 3 cut(s) 236, 295, 490
RseI CAYNNNNRTG 4 cut(s) 383, 501, 543, 672
SaqAI TTAA 2 cut(s) 71, 468
SatI GCNGC 2 cut(s) 13, 195
Sau3AI GATC 2 cut(s) 85, 387
Sau96I GGNCC 1 cut(s) 392
SchI GAGTC 3 cut(s) 257, 274, 709
ScrFI CCNGG 1 cut(s) 104
SduI GDGCHC 2 cut(s) 125, 550
SfaNI GCATC 2 cut(s) 13, 434
SfcI CTRYAG 1 cut(s) 192
SmiMI CAYNNNNRTG 4 cut(s) 383, 501, 543, 672
Sse9I AATT 3 cut(s) 27, 309, 464
SsiI CCGC 1 cut(s) 110
SspMI CTAG 2 cut(s) 656, 704
StyD4I CCNGG 1 cut(s) 102
StyI CCWWGG 1 cut(s) 40
TaaI ACNGT 2 cut(s) 370, 577
TaiI ACGT 1 cut(s) 495
TaqI TCGA 1 cut(s) 267
TasI AATT 3 cut(s) 27, 309, 464
Tru1I TTAA 2 cut(s) 71, 468
Tru9I TTAA 2 cut(s) 71, 468
TscAI CASTG 2 cut(s) 246, 595
TseFI GTSAC 2 cut(s) 277, 518
TseI GCWGC 2 cut(s) 12, 194
Tsp45I GTSAC 2 cut(s) 277, 518
TspDTI ATGAA 2 cut(s) 555, 700
TspGWI ACGGA 1 cut(s) 147
TspRI CASTG 2 cut(s) 246, 595
XapI RAATTY 2 cut(s) 309, 464
XceI RCATGY 1 cut(s) 136
XcmI CCANNNNNNNNNTGG 1 cut(s) 238
XspI CTAG 2 cut(s) 656, 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.