pycom05g21140

Belongs to the phosphoglycerate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0004015
Physical Location & Seq
Forward (+)
23828168 .. 23832149
3982 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g21140.1

Sequence Viewer

Length: 1737 bp
ATGCTAAGCATATTAAGTTATGACATCACCGCTCAACAGTTAAGATGTCTAGAAATCTTTTACCCCCCATCATATCACAGTTGCCACTTTTTTCAATCTCTCAGAAGCATCGAACACTTTGAACCTCTCCTCAACGATAAGGAGAAATCCACAACCCCCACACATACCACGCAATCAGTCCCATTGTTCTTCCCGTCCACATCTCTCCCAATCCACAAAAATCGAAAAAACAACCCCATTAACCTCTCCTCTCACTCGGTCAACCTCCGTTTACTATCCCACACCCCAACAGCAATGGCCTCCACCACCGCACCCACCACCTTCTCCCTCCTCCACTCCGCCACTGCTTCCACCTCCCGCACCCGCGCTTCCCTCTCCCATGTCTCGCTCCCCTCCTCCCTCAAACCCACTGCCCTCCGCCGCCTTGGCTTCTCCGCTGCCGACCCCCTCCTCGCTCTTCAAGTAGCTTCCAAAGTCCGATCCTTTGGATCCGGCAAAGGGGTCAGAGGCGTTGTTGCGATGGCGAAGAAGAGCGTCGGGGACCTTTCGGCGGCGGAGCTGAAGGGGAAGAAGGTGTTTGTCAGGGCTGACTTGAATGTACCTTTGGATGACAGCCAGAACATCACTGATGATACTAGAATCCGCGCTGCTATCCCCACCATTAAGCATTTGATTGAGAATGGGGCTAAAGTCATCCTTTCCAGTCATTTGGGACGACCAAAAGGTGTGACCCCAAAATTTAGCTTGGCACCTCTTGTACCTCGGCTTTCGGAGCTTCTTGGCATTCAGGTTGTCAAAGCTGAGGATTCAATTGGTCCAGAAGTAGAGAAGCTGGTGGCTTCACTTCCTGAAGGTAGTGTTCTTCTTCTTGAAAATGTGAGGTTTTACAAGGAAGAAGAGAAGAATGATCCTGAGCATGCAAAGAAGCTTGCCGCCTTAGCAGATCTTTTTGTTAACGATGCATTTGGGACTGCACATAGAGCTCATGCCTCAACTGAGGGAGTCACAAAATTCTTGAGGCCATCCGTAGCCGGTTTCCTTTTGCAGAAGGAACTCGACTATCTCGTTGGGGCAGTATCAAGCCCCAAAAGGCCATTTGCTGCCATTGTTGGTGGTTCAAAGGTCTCATCCAAGATCGGAGTGATTGAGTCACTTTTAGAGAAGGTTGATATCTTACTTCTTGGTGGAGGTATGATTTTCACATTTTACAAGGCACAAGGTCTCTCAGTCGGTTCATCTCTGGTAGAAGAAGATAAACTAGAACTTGCTACATCACTCATAGCAAAGGCCAAGGAAAAAGGAGTGTCTCTTTTGTTGCCCACTGATGTCGTAATTGCAGACAAGTTTGCTCCTGATGCAAACAGCAAGATTGTTCCAGCTTCAAGCATCCCTGATGGGTGGATGGGATTGGATATTGGTCCAGACTCTATTAAGACATTCAATGATGCACTGGATACCACTAAAACCATCATCTGGAATGGACCAATGGGAGTGTTTGAGTTTGACAAGTTCGCAGTAGGAACAGAGTCTGTTGCAAAGAAGCTTGCAGAACTTAGTGGGAAGGGAGTAACAACAATCATCGGAGGTGGAGATTCTGTTGCAGCCGTGGAGAAAGTAGGAGTCGCTAGTGTGATGAGCCACATATCAACTGGTGGTGGTGCTAGTTTGGAGCTGTTGGAAGGCAAAGAACTTCCTGGTGTACTTGCTCTTGACGAAGCCATACCAGTTCCTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002682 GO:0002697 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006464 GO:0006468 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009628 GO:0009941 GO:0009987 GO:0010035 GO:0010038 GO:0010319 GO:0015977 GO:0015979 GO:0016020 GO:0016051 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019253 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019538 GO:0019637 GO:0019685 GO:0019693 GO:0019752 GO:0030312 GO:0031347 GO:0031967 GO:0031975 GO:0032101 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0036211 GO:0042221 GO:0042866 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043900 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044267 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046939 GO:0048046 GO:0048583 GO:0050688 GO:0050691 GO:0050789 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0065007 GO:0071704 GO:0071944 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0080134 GO:0090407 GO:0140096 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
PGK

Protein Analysis

579

Amino Acids

61.43

Weight (kDa)

8.52

Isoelectric Point (pI)

39.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGK PF00162 184 - 562 1.1e-162 Phosphoglycerate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 748
AccB7I CCANNNNNTGG 1 cut(s) 1473
AccBSI CCGCTC 1 cut(s) 32
AccII CGCG 2 cut(s) 366, 645
AclWI GGATC 4 cut(s) 474, 483, 496, 902
AcsI RAATTY 2 cut(s) 737, 1010
AcuI CTGAAG 2 cut(s) 581, 870
AfaI GTAC 3 cut(s) 600, 759, 1701
AfiI CCNNNNNNNGG 3 cut(s) 498, 550, 1473
AgsI TTSAA 9 cut(s) 95, 122, 461, 595, 810, 872, 1119, 1383, 1441
AjnI CCWGG 1 cut(s) 1693
AjuI GAANNNNNNNTTGG 2 cut(s) 587, 619
Alw21I GWGCWC 1 cut(s) 985
Alw26I GTCTC 4 cut(s) 388, 1129, 1226, 1311
AlwI GGATC 4 cut(s) 474, 483, 496, 902
AlwNI CAGNNNCTG 2 cut(s) 1529, 1730
AoxI GGCC 4 cut(s) 297, 1019, 1091, 1287
ApeKI GCWGC 4 cut(s) 437, 647, 1100, 1601
ApoI RAATTY 2 cut(s) 737, 1010
AspLEI GCGC 2 cut(s) 368, 647
AspS9I GGNCC 4 cut(s) 541, 815, 1418, 1481
AsuHPI GGTGA 1 cut(s) 19
AvaII GGWCC 4 cut(s) 541, 815, 1418, 1481
BamHI GGATCC 1 cut(s) 488
BanI GGYRCC 1 cut(s) 748
BanII GRGCYC 1 cut(s) 985
Bbv12I GWGCWC 1 cut(s) 985
BbvCI CCTCAGC 1 cut(s) 801
BbvI GCAGC 4 cut(s) 424, 634, 1087, 1613
BccI CCATC 6 cut(s) 76, 514, 1030, 1388, 1396, 1475
BceAI ACGGC 1 cut(s) 1589
BciT130I CCWGG 1 cut(s) 1695
BciVI GTATCC 1 cut(s) 1447
BcoDI GTCTC 4 cut(s) 388, 1129, 1226, 1311
BfaI CTAG 5 cut(s) 50, 636, 1259, 1626, 1662
BfuI GTATCC 1 cut(s) 1447
BglI GCCNNNNNGGC 1 cut(s) 426
BglII AGATCT 1 cut(s) 943
BisI GCNGC 7 cut(s) 421, 438, 552, 648, 933, 1101, 1602
BlpI GCTNAGC 1 cut(s) 5
BlsI GCNGC 7 cut(s) 422, 439, 553, 649, 934, 1102, 1603
Bme1390I CCNGG 1 cut(s) 1695
Bme18I GGWCC 4 cut(s) 541, 815, 1418, 1481
BmgT120I GGNCC 4 cut(s) 541, 815, 1418, 1481
BmiI GGNNCC 3 cut(s) 490, 542, 750
BmrFI CCNGG 1 cut(s) 1695
BmsI GCATC 5 cut(s) 117, 949, 1345, 1395, 1435
Bpu10I CCTNAGC 3 cut(s) 801, 912, 937
Bpu1102I GCTNAGC 1 cut(s) 5
BpuEI CTTGAG 1 cut(s) 1036
BsaI GGTCTC 2 cut(s) 1129, 1226
BsaJI CCNNGG 4 cut(s) 424, 761, 1290, 1605
BsaXI ACNNNNNCTCC 2 cut(s) 1581, 1611
Bsc4I CCNNNNNNNGG 3 cut(s) 498, 550, 1473
Bse118I RCCGGY 1 cut(s) 1031
Bse1I ACTGG 4 cut(s) 702, 1455, 1654, 1724
Bse3DI GCAATG 1 cut(s) 300
BseBI CCWGG 1 cut(s) 1695
BseDI CCNNGG 4 cut(s) 424, 761, 1290, 1605
BseGI GGATG 6 cut(s) 613, 693, 1022, 1127, 1386, 1407
BseLI CCNNNNNNNGG 3 cut(s) 498, 550, 1473
BseMI GCAATG 1 cut(s) 300
BseMII CTCAG 5 cut(s) 115, 792, 903, 987, 1239
BseNI ACTGG 4 cut(s) 702, 1455, 1654, 1724
BseRI GAGGAG 5 cut(s) 119, 238, 320, 385, 440
BseXI GCAGC 4 cut(s) 424, 634, 1087, 1613
BsgI GTGCAG 1 cut(s) 957
Bsh1236I CGCG 2 cut(s) 366, 645
BshFI GGCC 4 cut(s) 299, 1021, 1093, 1289
BshNI GGYRCC 1 cut(s) 748
BsiHKAI GWGCWC 1 cut(s) 985
BsiSI CCGG 2 cut(s) 492, 1032
BslFI GGGAC 4 cut(s) 164, 554, 726, 982
BslI CCNNNNNNNGG 3 cut(s) 498, 550, 1473
BsmAI GTCTC 4 cut(s) 388, 1129, 1226, 1311
BsmFI GGGAC 4 cut(s) 164, 554, 726, 982
BsmI GAATGC 1 cut(s) 783
BsnI GGCC 4 cut(s) 299, 1021, 1093, 1289
Bso31I GGTCTC 2 cut(s) 1129, 1226
Bsp1286I GDGCHC 1 cut(s) 985
Bsp143I GATC 5 cut(s) 479, 488, 907, 943, 1134
Bsp1720I GCTNAGC 1 cut(s) 5
BspANI GGCC 4 cut(s) 299, 1021, 1093, 1289
BspCNI CTCAG 5 cut(s) 114, 793, 904, 988, 1238
BspFNI CGCG 2 cut(s) 366, 645
BspLI GGNNCC 3 cut(s) 490, 542, 750
BspPI GGATC 4 cut(s) 474, 483, 496, 902
BspQI GCTCTTC 2 cut(s) 462, 524
BspT107I GGYRCC 1 cut(s) 748
BspTNI GGTCTC 2 cut(s) 1129, 1226
BsrBI CCGCTC 1 cut(s) 32
BsrDI GCAATG 1 cut(s) 300
BsrFI RCCGGY 1 cut(s) 1031
BsrI ACTGG 4 cut(s) 702, 1455, 1654, 1724
BssAI RCCGGY 1 cut(s) 1031
BssECI CCNNGG 4 cut(s) 424, 761, 1290, 1605
BssMI GATC 5 cut(s) 479, 488, 907, 943, 1134
BssT1I CCWWGG 2 cut(s) 424, 1290
Bst2UI CCWGG 1 cut(s) 1695
Bst4CI ACNGT 2 cut(s) 39, 80
Bst6I CTCTTC 3 cut(s) 462, 524, 891
BstC8I GCNNGC 3 cut(s) 918, 930, 1545
BstDEI CTNAG 8 cut(s) 5, 101, 801, 912, 937, 996, 1225, 1553
BstDSI CCRYGG 1 cut(s) 1605
BstF5I GGATG 6 cut(s) 613, 693, 1022, 1127, 1386, 1407
BstFNI CGCG 2 cut(s) 366, 645
BstHHI GCGC 2 cut(s) 368, 647
BstKTI GATC 5 cut(s) 482, 491, 910, 946, 1137
BstMAI GTCTC 4 cut(s) 388, 1129, 1226, 1311
BstMBI GATC 5 cut(s) 479, 488, 907, 943, 1134
BstMWI GCNNNNNNNGC 5 cut(s) 426, 772, 938, 980, 1355
BstNI CCWGG 1 cut(s) 1695
BstNSI RCATGY 1 cut(s) 920
BstSCI CCNGG 1 cut(s) 1693
BstUI CGCG 2 cut(s) 366, 645
BstV1I GCAGC 4 cut(s) 424, 634, 1087, 1613
BstX2I RGATCY 2 cut(s) 488, 943
BstXI CCANNNNNNTGG 1 cut(s) 709
BstYI RGATCY 2 cut(s) 488, 943
BsuI GTATCC 1 cut(s) 1447
BsuRI GGCC 4 cut(s) 299, 1021, 1093, 1289
BtgI CCRYGG 1 cut(s) 1605
BtgZI GCGATG 1 cut(s) 533
BtsCI GGATG 6 cut(s) 613, 693, 1022, 1127, 1386, 1407
BtsI GCAGTG 2 cut(s) 342, 408
BtsIMutI CAGTG 5 cut(s) 342, 408, 624, 1320, 1448
Cac8I GCNNGC 3 cut(s) 918, 930, 1545
CaiI CAGNNNCTG 2 cut(s) 1529, 1730
CfoI GCGC 2 cut(s) 368, 647
Cfr10I RCCGGY 1 cut(s) 1031
Cfr13I GGNCC 4 cut(s) 541, 815, 1418, 1481
CseI GACGC 1 cut(s) 523
Csp6I GTAC 3 cut(s) 599, 758, 1700
CviAII CATG 3 cut(s) 380, 917, 986
CviQI GTAC 3 cut(s) 599, 758, 1700
DdeI CTNAG 8 cut(s) 5, 101, 801, 912, 937, 996, 1225, 1553
DpnI GATC 5 cut(s) 481, 490, 909, 945, 1136
DpnII GATC 5 cut(s) 479, 488, 907, 943, 1134
Eam1104I CTCTTC 3 cut(s) 462, 524, 891
EarI CTCTTC 3 cut(s) 462, 524, 891
EciI GGCGGA 3 cut(s) 328, 407, 569
Ecl136II GAGCTC 1 cut(s) 983
Eco130I CCWWGG 2 cut(s) 424, 1290
Eco24I GRGCYC 1 cut(s) 985
Eco31I GGTCTC 2 cut(s) 1129, 1226
Eco32I GATATC 1 cut(s) 1171
Eco47I GGWCC 4 cut(s) 541, 815, 1418, 1481
Eco53kI GAGCTC 1 cut(s) 983
Eco57I CTGAAG 2 cut(s) 581, 870
EcoICRI GAGCTC 1 cut(s) 983
EcoO109I RGGNCCY 1 cut(s) 541
EcoRII CCWGG 1 cut(s) 1693
EcoRV GATATC 1 cut(s) 1171
EcoT14I CCWWGG 2 cut(s) 424, 1290
EcoT22I ATGCAT 1 cut(s) 964
EcoT38I GRGCYC 1 cut(s) 985
ErhI CCWWGG 2 cut(s) 424, 1290
FaeI CATG 3 cut(s) 383, 920, 989
FalI AAGNNNNNCTT 2 cut(s) 681, 713
FaqI GGGAC 4 cut(s) 164, 554, 726, 982
FatI CATG 3 cut(s) 379, 916, 985
FauI CCCGC 2 cut(s) 365, 371
Fnu4HI GCNGC 7 cut(s) 421, 438, 552, 648, 933, 1101, 1602
FokI GGATG 6 cut(s) 620, 680, 1009, 1114, 1373, 1414
FriOI GRGCYC 1 cut(s) 985
Fsp4HI GCNGC 7 cut(s) 421, 438, 552, 648, 933, 1101, 1602
FspBI CTAG 5 cut(s) 50, 636, 1259, 1626, 1662
GlaI GCGC 2 cut(s) 367, 646
GluI GCNGC 7 cut(s) 421, 438, 552, 648, 933, 1101, 1602
HaeIII GGCC 4 cut(s) 299, 1021, 1093, 1289
HapII CCGG 2 cut(s) 492, 1032
HgaI GACGC 1 cut(s) 523
HhaI GCGC 2 cut(s) 368, 647
Hin1II CATG 3 cut(s) 383, 920, 989
Hin6I GCGC 2 cut(s) 366, 645
HinP1I GCGC 2 cut(s) 366, 645
HincII GTYRAC 2 cut(s) 262, 955
HindII GTYRAC 2 cut(s) 262, 955
HindIII AAGCTT 2 cut(s) 926, 1541
HinfI GANTC 8 cut(s) 639, 806, 1002, 1148, 1424, 1526, 1592, 1620
HpaI GTTAAC 1 cut(s) 955
HpaII CCGG 2 cut(s) 492, 1032
HphI GGTGA 1 cut(s) 19
Hpy166II GTNNAC 5 cut(s) 198, 262, 272, 955, 1700
Hpy188I TCNGA 6 cut(s) 104, 479, 506, 772, 1139, 1583
Hpy8I GTNNAC 5 cut(s) 198, 262, 272, 955, 1700
Hpy99I CGWCG 1 cut(s) 539
HpyAV CCTTC 8 cut(s) 331, 556, 565, 845, 1042, 1156, 1555, 1673
HpyCH4III ACNGT 2 cut(s) 39, 80
HpyF10VI GCNNNNNNNGC 5 cut(s) 426, 772, 938, 980, 1355
HpyF3I CTNAG 8 cut(s) 5, 101, 801, 912, 937, 996, 1225, 1553
Hsp92II CATG 3 cut(s) 383, 920, 989
HspAI GCGC 2 cut(s) 366, 645
KspAI GTTAAC 1 cut(s) 955
Kzo9I GATC 5 cut(s) 479, 488, 907, 943, 1134
LguI GCTCTTC 2 cut(s) 462, 524
LmnI GCTCC 5 cut(s) 393, 556, 772, 1354, 1669
Lsp1109I GCAGC 4 cut(s) 424, 634, 1087, 1613
LweI GCATC 5 cut(s) 117, 949, 1345, 1395, 1435
MaeI CTAG 5 cut(s) 50, 636, 1259, 1626, 1662
MaeIII GTNAC 4 cut(s) 727, 1003, 1149, 1567
MalI GATC 5 cut(s) 481, 490, 909, 945, 1136
MbiI CCGCTC 1 cut(s) 32
MboI GATC 5 cut(s) 479, 488, 907, 943, 1134
MfeI CAATTG 1 cut(s) 810
MflI RGATCY 2 cut(s) 488, 943
MhlI GDGCHC 1 cut(s) 985
MluCI AATT 4 cut(s) 737, 810, 1010, 1332
MlyI GAGTC 5 cut(s) 1011, 1157, 1418, 1535, 1629
MmeI TCCRAC 1 cut(s) 1656
Mph1103I ATGCAT 1 cut(s) 964
MseI TTAA 6 cut(s) 14, 41, 240, 663, 954, 1431
MspA1I CMGCKG 1 cut(s) 437
MspI CCGG 2 cut(s) 492, 1032
MspR9I CCNGG 1 cut(s) 1695
MunI CAATTG 1 cut(s) 810
Mva1269I GAATGC 1 cut(s) 783
MvaI CCWGG 1 cut(s) 1695
MvnI CGCG 2 cut(s) 366, 645
MwoI GCNNNNNNNGC 5 cut(s) 426, 772, 938, 980, 1355
NdeII GATC 5 cut(s) 479, 488, 907, 943, 1134
NlaIII CATG 3 cut(s) 383, 920, 989
NlaIV GGNNCC 3 cut(s) 490, 542, 750
NmeAIII GCCGAG 1 cut(s) 742
NmuCI GTSAC 3 cut(s) 727, 1003, 1149
NsiI ATGCAT 1 cut(s) 964
NspI RCATGY 1 cut(s) 920
PaeI GCATGC 1 cut(s) 920
PciSI GCTCTTC 2 cut(s) 462, 524
PcsI WCGNNNNNNNCGW 1 cut(s) 1062
PctI GAATGC 1 cut(s) 783
PfeI GAWTC 3 cut(s) 639, 806, 1592
PflMI CCANNNNNTGG 1 cut(s) 1473
PkrI GCNGC 7 cut(s) 422, 439, 553, 649, 934, 1102, 1603
PleI GAGTC 5 cut(s) 1010, 1156, 1418, 1534, 1628
PpsI GAGTC 5 cut(s) 1010, 1156, 1418, 1534, 1628
PpuMI RGGWCCY 1 cut(s) 541
Psp124BI GAGCTC 1 cut(s) 985
Psp5II RGGWCCY 1 cut(s) 541
Psp6I CCWGG 1 cut(s) 1693
PspGI CCWGG 1 cut(s) 1693
PspN4I GGNNCC 3 cut(s) 490, 542, 750
PspPI GGNCC 4 cut(s) 541, 815, 1418, 1481
PspPPI RGGWCCY 1 cut(s) 541
PstNI CAGNNNCTG 2 cut(s) 1529, 1730
PsuI RGATCY 2 cut(s) 488, 943
RsaI GTAC 3 cut(s) 600, 759, 1701
RsaNI GTAC 3 cut(s) 599, 758, 1700
SacI GAGCTC 1 cut(s) 985
SapI GCTCTTC 2 cut(s) 462, 524
SaqAI TTAA 6 cut(s) 14, 41, 240, 663, 954, 1431
SatI GCNGC 7 cut(s) 421, 438, 552, 648, 933, 1101, 1602
Sau3AI GATC 5 cut(s) 479, 488, 907, 943, 1134
Sau96I GGNCC 4 cut(s) 541, 815, 1418, 1481
SchI GAGTC 5 cut(s) 1011, 1157, 1418, 1535, 1629
ScrFI CCNGG 1 cut(s) 1695
SduI GDGCHC 1 cut(s) 985
SfaNI GCATC 5 cut(s) 117, 949, 1345, 1395, 1435
SinI GGWCC 4 cut(s) 541, 815, 1418, 1481
SmlI CTYRAG 1 cut(s) 1015
SmoI CTYRAG 1 cut(s) 1015
SphI GCATGC 1 cut(s) 920
Sse9I AATT 4 cut(s) 737, 810, 1010, 1332
SspMI CTAG 5 cut(s) 50, 636, 1259, 1626, 1662
SstI GAGCTC 1 cut(s) 985
StyD4I CCNGG 1 cut(s) 1693
StyI CCWWGG 2 cut(s) 424, 1290
TaaI ACNGT 2 cut(s) 39, 80
TaqI TCGA 3 cut(s) 111, 223, 1056
TaqII GACCGA 1 cut(s) 247
TasI AATT 4 cut(s) 737, 810, 1010, 1332
TatI WGTACW 1 cut(s) 1699
TauI GCSGC 3 cut(s) 423, 554, 935
TfiI GAWTC 3 cut(s) 639, 806, 1592
Tru1I TTAA 6 cut(s) 14, 41, 240, 663, 954, 1431
Tru9I TTAA 6 cut(s) 14, 41, 240, 663, 954, 1431
TscAI CASTG 5 cut(s) 349, 415, 631, 1327, 1455
TseFI GTSAC 3 cut(s) 727, 1003, 1149
TseI GCWGC 4 cut(s) 437, 647, 1100, 1601
Tsp45I GTSAC 3 cut(s) 727, 1003, 1149
TspDTI ATGAA 1 cut(s) 1224
TspGWI ACGGA 2 cut(s) 257, 1015
TspRI CASTG 5 cut(s) 349, 415, 631, 1327, 1455
Van91I CCANNNNNTGG 1 cut(s) 1473
VpaK11BI GGWCC 4 cut(s) 541, 815, 1418, 1481
XapI RAATTY 2 cut(s) 737, 1010
XbaI TCTAGA 1 cut(s) 49
XceI RCATGY 1 cut(s) 920
XcmI CCANNNNNNNNNTGG 1 cut(s) 1646
XspI CTAG 5 cut(s) 50, 636, 1259, 1626, 1662
Zsp2I ATGCAT 1 cut(s) 964
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.