Rh5DG266800

Belongs to the phosphoglycerate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0004015
Physical Location & Seq
Reverse (-)
32310065 .. 32312512
2448 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG266800.1

Sequence Viewer

Length: 771 bp
ATGTTCTGTAATTTGAAATTGGAGCGTGAATTTGGAAAGTTGAGTCTTGAAATTGTTTGTTCCGTACTTCATTCAGCCTGGATGTTGATGTTGCATGATGAAGACTTGAAAGGATTACTAATTCAAAGAACACAGGTTGTGAAGGCTGGAGATTCTATTGGTCTAGAAGTAGAAAAGCTGGCGGCTTCACTTCGTGATGGTGGTGTCCTTCTTCTTGAAAATGTGAGGTTTTACAAAGAGGAGGAGAAGAACGATCCTGAGCATGCAAAGAAGCTCGCCTCTGTAGCTGATCTTTTTGTCAATGATGCATTCGGAACTGCACATAGAACCCATGCTTCAACTGAGGGTGTAACAAAATTCTTAAGGCCATCTGTAGCTGGTTTTCTTTTGCAGAAGGCACAAGGTATCTCAGTGGGTTCATCTCTGGTGGAAAAGGATAAGCTAGAACTCGCTACATCACTCATTGCAAAGGCCAAGGCAAAGGGAGTGTCTCTTTTGCTACTCACTGATGTCATAATTGCAGACAAATTTGCTCCTGATGCAAACAGCAAGATTGTTCCAGCTTCAAGCATCCCTGATGGGTGGATGGGATTGGATATTGGACCAGACTCTATTAAGACATTCAATGATGCACTTGATACCACTCAAACCTTCATTTGGAACGGACCAATGGGAGTGTTTGAGTTTGACAAGTTTACAGTAGGAACAGAGGTAAGCAGTTGGCATTCATTTCTGTACATAAAAATGTTCACTACTTATATAGGTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002682 GO:0002697 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006464 GO:0006468 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009628 GO:0009941 GO:0009987 GO:0010035 GO:0010038 GO:0010319 GO:0015977 GO:0015979 GO:0016020 GO:0016051 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019253 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019538 GO:0019637 GO:0019685 GO:0019693 GO:0019752 GO:0030312 GO:0031347 GO:0031967 GO:0031975 GO:0032101 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0036211 GO:0042221 GO:0042866 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043900 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044267 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046939 GO:0048046 GO:0048583 GO:0050688 GO:0050691 GO:0050789 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0065007 GO:0071704 GO:0071944 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0080134 GO:0090407 GO:0140096 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
PGK

Protein Analysis

256

Amino Acids

28.06

Weight (kDa)

5.56

Isoelectric Point (pI)

22.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGK PF00162 32 - 132 1e-31 Phosphoglycerate kinase
PGK PF00162 131 - 237 5.9e-32 Phosphoglycerate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 182
AclWI GGATC 1 cut(s) 248
AcsI RAATTY 3 cut(s) 29, 356, 527
AdeI CACNNNGTG 1 cut(s) 194
AfaI GTAC 2 cut(s) 66, 737
AfiI CCNNNNNNNGG 1 cut(s) 657
AflII CTTAAG 1 cut(s) 361
AgsI TTSAA 8 cut(s) 16, 50, 109, 125, 218, 339, 567, 625
AjnI CCWGG 1 cut(s) 77
AluBI AGCT 6 cut(s) 178, 274, 287, 377, 442, 563
AluI AGCT 6 cut(s) 178, 274, 287, 377, 442, 563
Alw26I GTCTC 1 cut(s) 495
AlwI GGATC 1 cut(s) 248
AoxI GGCC 2 cut(s) 365, 471
ApoI RAATTY 3 cut(s) 29, 356, 527
ArsI GACNNNNNNTTYG 2 cut(s) 473, 505
AspS9I GGNCC 2 cut(s) 602, 665
AvaII GGWCC 2 cut(s) 602, 665
BbsI GAAGAC 1 cut(s) 108
BccI CCATC 4 cut(s) 191, 376, 572, 580
BciT130I CCWGG 1 cut(s) 79
BcoDI GTCTC 1 cut(s) 495
BfaI CTAG 2 cut(s) 164, 443
BfmI CTRYAG 2 cut(s) 282, 372
BfrI CTTAAG 1 cut(s) 361
BisI GCNGC 1 cut(s) 183
BlsI GCNGC 1 cut(s) 184
Bme1390I CCNGG 1 cut(s) 79
Bme18I GGWCC 2 cut(s) 602, 665
BmgT120I GGNCC 2 cut(s) 602, 665
BmrFI CCNGG 1 cut(s) 79
BmsI GCATC 4 cut(s) 295, 529, 579, 619
BpiI GAAGAC 1 cut(s) 108
BpmI CTGGAG 1 cut(s) 168
Bpu10I CCTNAGC 1 cut(s) 258
BsaJI CCNNGG 1 cut(s) 474
Bsc4I CCNNNNNNNGG 1 cut(s) 657
Bse3DI GCAATG 1 cut(s) 462
BseBI CCWGG 1 cut(s) 79
BseDI CCNNGG 1 cut(s) 474
BseGI GGATG 3 cut(s) 87, 570, 591
BseLI CCNNNNNNNGG 1 cut(s) 657
BseMI GCAATG 1 cut(s) 462
BseMII CTCAG 3 cut(s) 249, 333, 423
BseRI GAGGAG 2 cut(s) 254, 257
BsgI GTGCAG 1 cut(s) 303
BshFI GGCC 2 cut(s) 367, 473
BslI CCNNNNNNNGG 1 cut(s) 657
BsmAI GTCTC 1 cut(s) 495
BsmI GAATGC 2 cut(s) 308, 724
BsnI GGCC 2 cut(s) 367, 473
Bsp1407I TGTACA 1 cut(s) 735
Bsp143I GATC 2 cut(s) 253, 289
BspACI CCGC 1 cut(s) 182
BspANI GGCC 2 cut(s) 367, 473
BspCNI CTCAG 3 cut(s) 250, 334, 422
BspPI GGATC 1 cut(s) 248
BspTI CTTAAG 1 cut(s) 361
BsrDI GCAATG 1 cut(s) 462
BsrGI TGTACA 1 cut(s) 735
BssECI CCNNGG 1 cut(s) 474
BssMI GATC 2 cut(s) 253, 289
BssT1I CCWWGG 1 cut(s) 474
Bst2UI CCWGG 1 cut(s) 79
Bst4CI ACNGT 1 cut(s) 700
BstAFI CTTAAG 1 cut(s) 361
BstAUI TGTACA 1 cut(s) 735
BstC8I GCNNGC 3 cut(s) 180, 264, 276
BstDEI CTNAG 4 cut(s) 258, 342, 409, 768
BstF5I GGATG 3 cut(s) 87, 570, 591
BstKTI GATC 2 cut(s) 256, 292
BstMAI GTCTC 1 cut(s) 495
BstMBI GATC 2 cut(s) 253, 289
BstMWI GCNNNNNNNGC 2 cut(s) 284, 539
BstNI CCWGG 1 cut(s) 79
BstNSI RCATGY 1 cut(s) 266
BstSCI CCNGG 1 cut(s) 77
BstSFI CTRYAG 2 cut(s) 282, 372
BstV2I GAAGAC 1 cut(s) 108
BsuRI GGCC 2 cut(s) 367, 473
BtsCI GGATG 3 cut(s) 87, 570, 591
BtsIMutI CAGTG 2 cut(s) 417, 504
Cac8I GCNNGC 3 cut(s) 180, 264, 276
Cfr13I GGNCC 2 cut(s) 602, 665
Csp6I GTAC 2 cut(s) 65, 736
CviAII CATG 3 cut(s) 95, 263, 332
CviQI GTAC 2 cut(s) 65, 736
DdeI CTNAG 4 cut(s) 258, 342, 409, 768
DpnI GATC 2 cut(s) 255, 291
DpnII GATC 2 cut(s) 253, 289
DraIII CACNNNGTG 1 cut(s) 194
Eco130I CCWWGG 1 cut(s) 474
Eco47I GGWCC 2 cut(s) 602, 665
EcoRII CCWGG 1 cut(s) 77
EcoT14I CCWWGG 1 cut(s) 474
EcoT22I ATGCAT 1 cut(s) 310
ErhI CCWWGG 1 cut(s) 474
FaeI CATG 3 cut(s) 98, 266, 335
FaiI YATR 8 cut(s) 96, 264, 324, 333, 515, 740, 759, 761
FatI CATG 3 cut(s) 94, 262, 331
Fnu4HI GCNGC 1 cut(s) 183
FokI GGATG 3 cut(s) 94, 557, 598
Fsp4HI GCNGC 1 cut(s) 183
FspBI CTAG 2 cut(s) 164, 443
GluI GCNGC 1 cut(s) 183
GsuI CTGGAG 1 cut(s) 168
HaeIII GGCC 2 cut(s) 367, 473
Hin1II CATG 3 cut(s) 98, 266, 335
HinfI GANTC 3 cut(s) 43, 152, 608
Hpy166II GTNNAC 2 cut(s) 696, 750
Hpy188I TCNGA 1 cut(s) 314
Hpy188III TCNNGA 6 cut(s) 47, 164, 194, 215, 257, 536
Hpy8I GTNNAC 2 cut(s) 696, 750
HpyAV CCTTC 4 cut(s) 136, 218, 388, 661
HpyCH4III ACNGT 1 cut(s) 700
HpyCH4V TGCA 9 cut(s) 94, 266, 308, 320, 391, 467, 521, 542, 632
HpyF10VI GCNNNNNNNGC 2 cut(s) 284, 539
HpyF3I CTNAG 4 cut(s) 258, 342, 409, 768
Hsp92II CATG 3 cut(s) 98, 266, 335
Kzo9I GATC 2 cut(s) 253, 289
LmnI GCTCC 2 cut(s) 22, 538
LweI GCATC 4 cut(s) 295, 529, 579, 619
MaeI CTAG 2 cut(s) 164, 443
MaeIII GTNAC 1 cut(s) 349
MalI GATC 2 cut(s) 255, 291
MboI GATC 2 cut(s) 253, 289
MboII GAAGA 3 cut(s) 113, 203, 259
MluCI AATT 8 cut(s) 10, 17, 29, 51, 120, 356, 516, 527
MlyI GAGTC 2 cut(s) 52, 602
MnlI CCTC 6 cut(s) 219, 232, 235, 289, 337, 703
Mph1103I ATGCAT 1 cut(s) 310
MseI TTAA 2 cut(s) 362, 615
MslI CAYNNNNRTG 1 cut(s) 743
MspCI CTTAAG 1 cut(s) 361
MspR9I CCNGG 1 cut(s) 79
Mva1269I GAATGC 2 cut(s) 308, 724
MvaI CCWGG 1 cut(s) 79
MwoI GCNNNNNNNGC 2 cut(s) 284, 539
NdeII GATC 2 cut(s) 253, 289
NlaIII CATG 3 cut(s) 98, 266, 335
NsiI ATGCAT 1 cut(s) 310
NspI RCATGY 1 cut(s) 266
PaeI GCATGC 1 cut(s) 266
PctI GAATGC 2 cut(s) 308, 724
PfeI GAWTC 1 cut(s) 152
PkrI GCNGC 1 cut(s) 184
PleI GAGTC 2 cut(s) 51, 602
PpsI GAGTC 2 cut(s) 51, 602
Psp6I CCWGG 1 cut(s) 77
PspGI CCWGG 1 cut(s) 77
PspPI GGNCC 2 cut(s) 602, 665
RsaI GTAC 2 cut(s) 66, 737
RsaNI GTAC 2 cut(s) 65, 736
RseI CAYNNNNRTG 1 cut(s) 743
SaqAI TTAA 2 cut(s) 362, 615
SatI GCNGC 1 cut(s) 183
Sau3AI GATC 2 cut(s) 253, 289
Sau96I GGNCC 2 cut(s) 602, 665
SchI GAGTC 2 cut(s) 52, 602
ScrFI CCNGG 1 cut(s) 79
SfaNI GCATC 4 cut(s) 295, 529, 579, 619
SfcI CTRYAG 2 cut(s) 282, 372
SinI GGWCC 2 cut(s) 602, 665
SmiMI CAYNNNNRTG 1 cut(s) 743
SmlI CTYRAG 1 cut(s) 361
SmoI CTYRAG 1 cut(s) 361
SphI GCATGC 1 cut(s) 266
Sse9I AATT 8 cut(s) 10, 17, 29, 51, 120, 356, 516, 527
SsiI CCGC 1 cut(s) 182
SspMI CTAG 2 cut(s) 164, 443
StyD4I CCNGG 1 cut(s) 77
StyI CCWWGG 1 cut(s) 474
TaaI ACNGT 1 cut(s) 700
TasI AATT 8 cut(s) 10, 17, 29, 51, 120, 356, 516, 527
TatI WGTACW 1 cut(s) 735
TauI GCSGC 1 cut(s) 185
TfiI GAWTC 1 cut(s) 152
Tru1I TTAA 2 cut(s) 362, 615
Tru9I TTAA 2 cut(s) 362, 615
TscAI CASTG 2 cut(s) 417, 511
TspDTI ATGAA 5 cut(s) 59, 114, 408, 643, 717
TspGWI ACGGA 2 cut(s) 52, 678
TspRI CASTG 2 cut(s) 417, 511
Vha464I CTTAAG 1 cut(s) 361
VpaK11BI GGWCC 2 cut(s) 602, 665
XapI RAATTY 3 cut(s) 29, 356, 527
XbaI TCTAGA 1 cut(s) 163
XceI RCATGY 1 cut(s) 266
XspI CTAG 2 cut(s) 164, 443
Zsp2I ATGCAT 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.