pycom05g27860
MYB Family

Myb/SANT-like DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003314
Physical Location & Seq
Forward (+)
28739241 .. 28741737
2497 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g27860.1

Sequence Viewer

Length: 930 bp
ATGGGCGACCTAACGGACCTCCAAACGACGCCGTCCAATGCCACCTCCACCCCTCCCTCCTCCAACCCTCGCCCCATGCCCGTCCGCGAAGACTGCTGGAGCGAGGACGCCACCTCCACTCTCGTCGACGCCTGGGGCCGCCGCTACCTCGAGCTCAACCGCGGGAACCTCCGCCAGAAGGACTGGCAGGAGGTCGCTGACGCCGTCAACGCCCTCCACGGCCACACCAAGAAGACCCACCGGACGGACGTTCAGTGCAAGAACCGAATCGATACAATTAAGAAGAAGTACAAGGTGGAGAAGGCTAGGGTTTCGTCCTCCAACGGAACCCTAACCTCGTCGTGGCCTTTCTTCGAGAGGCTCGACTCCCTCATCGGATCTAATGTCAAGAAGCCGTCTCCCTCGCCTCTCTCGCCTTCGCCGCCGGTCGCCGTTCCGTTGCCTTTGGGGTACCGGAAGACGCCGTCCTCGGCTCCGGTGGTGACGGCTGTGGCGCTTCCGCAGAAGCGGTCGGCGTCGACTGCGGCGCTCGACGAGGGTTTCTTTAGGATGAATTACTCGGCTGTGGCTGCGGCTGCAGCCGCGGGGGCTGAGGACGATGACGACGAGGATGAGGATGAGGAGGATGAGGATAACGATGAGGTCGATGAGGAGGTTGAGGTGGAGAGGGAGAGGGAGAGCGGAGGCGAGATTGGAGGCGGGGGAGGAGAAGGGTTGAAGAGATTGGCGAGAGCGATTGAGAGGTTCGGAGAGGTGTATCAGAGAGTCGAGGCCGAGAAGTTGAGGCAGATGGTGGAGTTGGAGAAGCAGAGGATCCAATTCGCCAATGAATTGGAGGTAGAGAGGATGAGGATGATCATGGACACGCAGGTCCAGCTCGAACGAATTAAGCACGGCAAGCGATCGGGCTCTAATGATATCTTTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-bind_4

Protein Analysis

310

Amino Acids

34.35

Weight (kDa)

5.06

Isoelectric Point (pI)

46.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-bind_4 PF13837 31 - 124 6.6e-20 Myb/SANT-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 869
Acc36I ACCTGC 1 cut(s) 859
Acc65I GGTACC 1 cut(s) 450
AccB1I GGYRCC 1 cut(s) 450
AccBSI CCGCTC 1 cut(s) 681
AccI GTMKAC 2 cut(s) 126, 518
AccII CGCG 3 cut(s) 87, 162, 584
AclWI GGATC 3 cut(s) 385, 808, 821
AcoI YGGCCR 1 cut(s) 220
AcyI GRCGYC 6 cut(s) 29, 108, 129, 201, 461, 515
AfaI GTAC 2 cut(s) 290, 452
AfiI CCNNNNNNNGG 3 cut(s) 178, 244, 342
AgsI TTSAA 1 cut(s) 718
AjnI CCWGG 1 cut(s) 131
AluBI AGCT 3 cut(s) 154, 877, 927
AluI AGCT 3 cut(s) 154, 877, 927
Alw21I GWGCWC 1 cut(s) 156
Alw26I GTCTC 1 cut(s) 402
AlwI GGATC 3 cut(s) 385, 808, 821
Ama87I CYCGRG 1 cut(s) 149
AoxI GGCC 4 cut(s) 136, 220, 344, 771
ApeKI GCWGC 3 cut(s) 569, 575, 578
Asp718I GGTACC 1 cut(s) 450
AspLEI GCGC 2 cut(s) 496, 529
AspS9I GGNCC 3 cut(s) 16, 136, 871
AsuHPI GGTGA 1 cut(s) 493
AvaI CYCGRG 1 cut(s) 149
AvaII GGWCC 2 cut(s) 16, 871
BamHI GGATCC 1 cut(s) 813
BanI GGYRCC 1 cut(s) 450
BanII GRGCYC 2 cut(s) 156, 911
BbsI GAAGAC 3 cut(s) 96, 239, 464
Bbv12I GWGCWC 1 cut(s) 156
BbvCI CCTCAGC 1 cut(s) 591
BbvI GCAGC 3 cut(s) 556, 562, 590
BccI CCATC 1 cut(s) 784
BceAI ACGGC 8 cut(s) 16, 188, 235, 379, 416, 448, 501, 910
BciT130I CCWGG 1 cut(s) 133
BclI TGATCA 1 cut(s) 855
BcoDI GTCTC 1 cut(s) 402
BfaI CTAG 2 cut(s) 306, 928
BfmI CTRYAG 1 cut(s) 576
BfoI RGCGCY 2 cut(s) 497, 530
BfuAI ACCTGC 1 cut(s) 859
BglI GCCNNNNNGGC 1 cut(s) 587
BisI GCNGC 9 cut(s) 139, 142, 422, 525, 570, 573, 576, 579, 582
BlsI GCNGC 9 cut(s) 140, 143, 423, 526, 571, 574, 577, 580, 583
Bme1390I CCNGG 1 cut(s) 133
Bme18I GGWCC 2 cut(s) 16, 871
BmeT110I CYCGRG 1 cut(s) 149
BmgT120I GGNCC 3 cut(s) 16, 136, 871
BmiI GGNNCC 6 cut(s) 137, 167, 328, 452, 474, 815
BmrFI CCNGG 1 cut(s) 133
BpiI GAAGAC 3 cut(s) 96, 239, 464
BpmI CTGGAG 1 cut(s) 118
Bpu10I CCTNAGC 1 cut(s) 591
Bsa29I ATCGAT 1 cut(s) 270
BsaHI GRCGYC 6 cut(s) 29, 108, 129, 201, 461, 515
BsaJI CCNNGG 5 cut(s) 132, 160, 217, 468, 582
BsaWI WCCGGW 3 cut(s) 240, 453, 475
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bsc4I CCNNNNNNNGG 3 cut(s) 178, 244, 342
Bse118I RCCGGY 1 cut(s) 424
Bse1I ACTGG 1 cut(s) 188
BseBI CCWGG 1 cut(s) 133
BseCI ATCGAT 1 cut(s) 270
BseDI CCNNGG 5 cut(s) 132, 160, 217, 468, 582
BseGI GGATG 6 cut(s) 555, 616, 622, 631, 852, 858
BseLI CCNNNNNNNGG 3 cut(s) 178, 244, 342
BseMII CTCAG 1 cut(s) 582
BseNI ACTGG 1 cut(s) 188
BseRI GAGGAG 4 cut(s) 49, 635, 665, 720
BseXI GCAGC 3 cut(s) 556, 562, 590
Bsh1236I CGCG 3 cut(s) 87, 162, 584
Bsh1285I CGRYCG 3 cut(s) 429, 512, 905
BshFI GGCC 4 cut(s) 138, 222, 346, 773
BshNI GGYRCC 1 cut(s) 450
BshVI ATCGAT 1 cut(s) 270
BsiEI CGRYCG 3 cut(s) 429, 512, 905
BsiHKAI GWGCWC 1 cut(s) 156
BsiHKCI CYCGRG 1 cut(s) 149
BsiSI CCGG 4 cut(s) 241, 425, 454, 476
BslI CCNNNNNNNGG 3 cut(s) 178, 244, 342
BsmAI GTCTC 1 cut(s) 402
BsmBI CGTCTC 1 cut(s) 402
BsnI GGCC 4 cut(s) 138, 222, 346, 773
BsoBI CYCGRG 1 cut(s) 149
Bsp1286I GDGCHC 2 cut(s) 156, 911
Bsp143I GATC 4 cut(s) 377, 813, 855, 902
BspANI GGCC 4 cut(s) 138, 222, 346, 773
BspCNI CTCAG 1 cut(s) 583
BspDI ATCGAT 1 cut(s) 270
BspFNI CGCG 3 cut(s) 87, 162, 584
BspLI GGNNCC 6 cut(s) 137, 167, 328, 452, 474, 815
BspMAI CTGCAG 1 cut(s) 580
BspMI ACCTGC 1 cut(s) 859
BspPI GGATC 3 cut(s) 385, 808, 821
BspT107I GGYRCC 1 cut(s) 450
BsrBI CCGCTC 1 cut(s) 681
BsrFI RCCGGY 1 cut(s) 424
BsrI ACTGG 1 cut(s) 188
BssAI RCCGGY 1 cut(s) 424
BssECI CCNNGG 5 cut(s) 132, 160, 217, 468, 582
BssMI GATC 4 cut(s) 377, 813, 855, 902
BssNI GRCGYC 6 cut(s) 29, 108, 129, 201, 461, 515
Bst2UI CCWGG 1 cut(s) 133
Bst6I CTCTTC 1 cut(s) 713
BstACI GRCGYC 6 cut(s) 29, 108, 129, 201, 461, 515
BstC8I GCNNGC 1 cut(s) 899
BstDEI CTNAG 1 cut(s) 591
BstDSI CCRYGG 3 cut(s) 160, 217, 582
BstF5I GGATG 6 cut(s) 555, 616, 622, 631, 852, 858
BstFNI CGCG 3 cut(s) 87, 162, 584
BstH2I RGCGCY 2 cut(s) 497, 530
BstHHI GCGC 2 cut(s) 496, 529
BstKTI GATC 4 cut(s) 380, 816, 858, 905
BstMAI GTCTC 1 cut(s) 402
BstMBI GATC 4 cut(s) 377, 813, 855, 902
BstMCI CGRYCG 3 cut(s) 429, 512, 905
BstNI CCWGG 1 cut(s) 133
BstSCI CCNGG 1 cut(s) 131
BstSFI CTRYAG 1 cut(s) 576
BstUI CGCG 3 cut(s) 87, 162, 584
BstV1I GCAGC 3 cut(s) 556, 562, 590
BstV2I GAAGAC 3 cut(s) 96, 239, 464
BstX2I RGATCY 2 cut(s) 377, 813
BstXI CCANNNNNNTGG 1 cut(s) 832
BstYI RGATCY 2 cut(s) 377, 813
Bsu15I ATCGAT 1 cut(s) 270
BsuRI GGCC 4 cut(s) 138, 222, 346, 773
BsuTUI ATCGAT 1 cut(s) 270
BtgI CCRYGG 3 cut(s) 160, 217, 582
BtsCI GGATG 6 cut(s) 555, 616, 622, 631, 852, 858
BtsIMutI CAGTG 1 cut(s) 260
BveI ACCTGC 1 cut(s) 859
Cac8I GCNNGC 1 cut(s) 899
CfoI GCGC 2 cut(s) 496, 529
Cfr10I RCCGGY 1 cut(s) 424
Cfr13I GGNCC 3 cut(s) 16, 136, 871
Cfr42I CCGCGG 2 cut(s) 163, 585
ClaI ATCGAT 1 cut(s) 270
CseI GACGC 6 cut(s) 37, 116, 137, 209, 469, 504
Csp6I GTAC 2 cut(s) 289, 451
CviAII CATG 2 cut(s) 76, 859
CviQI GTAC 2 cut(s) 289, 451
DdeI CTNAG 1 cut(s) 591
DpnI GATC 4 cut(s) 379, 815, 857, 904
DpnII GATC 4 cut(s) 377, 813, 855, 902
DrdI GACNNNNNNGTC 1 cut(s) 869
DseDI GACNNNNNNGTC 1 cut(s) 869
EaeI YGGCCR 1 cut(s) 220
Eam1104I CTCTTC 1 cut(s) 713
EarI CTCTTC 1 cut(s) 713
EciI GGCGGA 1 cut(s) 161
Ecl136II GAGCTC 1 cut(s) 154
Eco24I GRGCYC 2 cut(s) 156, 911
Eco32I GATATC 1 cut(s) 919
Eco47I GGWCC 2 cut(s) 16, 871
Eco53kI GAGCTC 1 cut(s) 154
Eco88I CYCGRG 1 cut(s) 149
EcoICRI GAGCTC 1 cut(s) 154
EcoRII CCWGG 1 cut(s) 131
EcoRV GATATC 1 cut(s) 919
EcoT38I GRGCYC 2 cut(s) 156, 911
Esp3I CGTCTC 1 cut(s) 402
FaeI CATG 2 cut(s) 79, 862
FaiI YATR 2 cut(s) 77, 860
FatI CATG 2 cut(s) 75, 858
FauI CCCGC 3 cut(s) 155, 577, 692
FbaI TGATCA 1 cut(s) 855
FblI GTMKAC 2 cut(s) 126, 518
Fnu4HI GCNGC 9 cut(s) 139, 142, 422, 525, 570, 573, 576, 579, 582
FokI GGATG 6 cut(s) 562, 623, 629, 638, 859, 865
FriOI GRGCYC 2 cut(s) 156, 911
Fsp4HI GCNGC 9 cut(s) 139, 142, 422, 525, 570, 573, 576, 579, 582
FspBI CTAG 2 cut(s) 306, 928
GlaI GCGC 2 cut(s) 495, 528
GluI GCNGC 9 cut(s) 139, 142, 422, 525, 570, 573, 576, 579, 582
GsuI CTGGAG 1 cut(s) 118
HaeII RGCGCY 2 cut(s) 497, 530
HaeIII GGCC 4 cut(s) 138, 222, 346, 773
HapII CCGG 4 cut(s) 241, 425, 454, 476
HgaI GACGC 6 cut(s) 37, 116, 137, 209, 469, 504
HhaI GCGC 2 cut(s) 496, 529
Hin1I GRCGYC 6 cut(s) 29, 108, 129, 201, 461, 515
Hin1II CATG 2 cut(s) 79, 862
Hin6I GCGC 2 cut(s) 494, 527
HinP1I GCGC 2 cut(s) 494, 527
HincII GTYRAC 3 cut(s) 127, 208, 519
HindII GTYRAC 3 cut(s) 127, 208, 519
HinfI GANTC 3 cut(s) 267, 365, 765
HpaII CCGG 4 cut(s) 241, 425, 454, 476
HphI GGTGA 1 cut(s) 493
Hpy166II GTNNAC 3 cut(s) 127, 208, 519
Hpy188I TCNGA 3 cut(s) 377, 749, 762
Hpy188III TCNNGA 2 cut(s) 355, 388
Hpy8I GTNNAC 3 cut(s) 127, 208, 519
Hpy99I CGWCG 7 cut(s) 31, 128, 131, 343, 520, 536, 608
HpyAV CCTTC 4 cut(s) 172, 295, 426, 704
HpyCH4IV ACGT 1 cut(s) 249
HpyCH4V TGCA 2 cut(s) 258, 578
HpyF3I CTNAG 1 cut(s) 591
HpySE526I ACGT 1 cut(s) 249
Hsp92I GRCGYC 6 cut(s) 29, 108, 129, 201, 461, 515
Hsp92II CATG 2 cut(s) 79, 862
HspAI GCGC 2 cut(s) 494, 527
KpnI GGTACC 1 cut(s) 454
Ksp22I TGATCA 1 cut(s) 855
KspI CCGCGG 2 cut(s) 163, 585
Kzo9I GATC 4 cut(s) 377, 813, 855, 902
LmnI GCTCC 2 cut(s) 99, 478
Lsp1109I GCAGC 3 cut(s) 556, 562, 590
MaeI CTAG 2 cut(s) 306, 928
MaeII ACGT 1 cut(s) 249
MaeIII GTNAC 1 cut(s) 481
MalI GATC 4 cut(s) 379, 815, 857, 904
MbiI CCGCTC 1 cut(s) 681
MboI GATC 4 cut(s) 377, 813, 855, 902
MboII GAAGA 6 cut(s) 101, 244, 295, 343, 469, 730
MflI RGATCY 2 cut(s) 377, 813
MhlI GDGCHC 2 cut(s) 156, 911
MluCI AATT 5 cut(s) 276, 553, 818, 830, 885
MlyI GAGTC 2 cut(s) 359, 774
MmeI TCCRAC 3 cut(s) 87, 345, 780
MseI TTAA 2 cut(s) 279, 888
MspA1I CMGCKG 2 cut(s) 162, 584
MspI CCGG 4 cut(s) 241, 425, 454, 476
MspR9I CCNGG 1 cut(s) 133
MvaI CCWGG 1 cut(s) 133
MvnI CGCG 3 cut(s) 87, 162, 584
NdeII GATC 4 cut(s) 377, 813, 855, 902
NlaIII CATG 2 cut(s) 79, 862
NlaIV GGNNCC 6 cut(s) 137, 167, 328, 452, 474, 815
NmeAIII GCCGAG 3 cut(s) 449, 539, 799
NmuCI GTSAC 1 cut(s) 481
PaeR7I CTCGAG 1 cut(s) 149
PcsI WCGNNNNNNNCGW 4 cut(s) 201, 360, 603, 642
PfeI GAWTC 1 cut(s) 267
PflFI GACNNNGTC 3 cut(s) 31, 203, 463
PkrI GCNGC 9 cut(s) 140, 143, 423, 526, 571, 574, 577, 580, 583
Ple19I CGATCG 1 cut(s) 905
PleI GAGTC 2 cut(s) 359, 773
PpsI GAGTC 2 cut(s) 359, 773
Psp124BI GAGCTC 1 cut(s) 156
Psp6I CCWGG 1 cut(s) 131
PspGI CCWGG 1 cut(s) 131
PspN4I GGNNCC 6 cut(s) 137, 167, 328, 452, 474, 815
PspPI GGNCC 3 cut(s) 16, 136, 871
PspXI VCTCGAGB 1 cut(s) 149
PstI CTGCAG 1 cut(s) 580
PsuI RGATCY 2 cut(s) 377, 813
PsyI GACNNNGTC 3 cut(s) 31, 203, 463
PvuI CGATCG 1 cut(s) 905
RsaI GTAC 2 cut(s) 290, 452
RsaNI GTAC 2 cut(s) 289, 451
SacI GAGCTC 1 cut(s) 156
SacII CCGCGG 2 cut(s) 163, 585
SalI GTCGAC 2 cut(s) 125, 517
SaqAI TTAA 2 cut(s) 279, 888
SatI GCNGC 9 cut(s) 139, 142, 422, 525, 570, 573, 576, 579, 582
Sau3AI GATC 4 cut(s) 377, 813, 855, 902
Sau96I GGNCC 3 cut(s) 16, 136, 871
SchI GAGTC 2 cut(s) 359, 774
ScrFI CCNGG 1 cut(s) 133
SduI GDGCHC 2 cut(s) 156, 911
SfcI CTRYAG 1 cut(s) 576
Sfr274I CTCGAG 1 cut(s) 149
Sfr303I CCGCGG 2 cut(s) 163, 585
SgrBI CCGCGG 2 cut(s) 163, 585
SgrDI CGTCGACG 1 cut(s) 125
SinI GGWCC 2 cut(s) 16, 871
SlaI CTCGAG 1 cut(s) 149
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
Sse9I AATT 5 cut(s) 276, 553, 818, 830, 885
SspMI CTAG 2 cut(s) 306, 928
SstI GAGCTC 1 cut(s) 156
StyD4I CCNGG 1 cut(s) 131
TaiI ACGT 1 cut(s) 252
TasI AATT 5 cut(s) 276, 553, 818, 830, 885
TatI WGTACW 1 cut(s) 288
TauI GCSGC 6 cut(s) 141, 144, 424, 527, 575, 584
TfiI GAWTC 1 cut(s) 267
Tru1I TTAA 2 cut(s) 279, 888
Tru9I TTAA 2 cut(s) 279, 888
TscAI CASTG 1 cut(s) 260
TseFI GTSAC 1 cut(s) 481
TseI GCWGC 3 cut(s) 569, 575, 578
Tsp45I GTSAC 1 cut(s) 481
TspDTI ATGAA 2 cut(s) 566, 843
TspGWI ACGGA 4 cut(s) 29, 260, 339, 426
TspRI CASTG 1 cut(s) 260
Tth111I GACNNNGTC 3 cut(s) 31, 203, 463
VpaK11BI GGWCC 2 cut(s) 16, 871
XhoI CTCGAG 1 cut(s) 149
XmiI GTMKAC 2 cut(s) 126, 518
XspI CTAG 2 cut(s) 306, 928
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.