pycom05g28660

HB1, ASXL, restriction endonuclease HTH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0011708
Physical Location & Seq
Forward (+)
29299370 .. 29309552
10183 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 5655 bp
ATGGAAGGTGGTGGAATTAGAATTAGAGCGAGGAGGAAATACTGTCGTTGTTTTAAAAAACATGTTCCTCGTCCTGATTTTCCCTTTTCCTATTTTTCGATATTATTAATATTATTCGTTGTTTTTGTTTTTGATGTTAATGTTTGTTGCTGTGTTTTCTTTTCAATCCCCGTAAAAAATTCAACATTTATTATTCGTAAATGTTTATTCTTCGTATTCTTAATATTCCATCATGATAATTCTACGCCTCTCTTTCTTCTCGATCTCTTCTTCCTTGACTGTTACGGTATTTTGTTTTTAGAGAGAAAAAGAACGAACGAGAGGATTGAAAGACGGAAGATTTGGGTGGAATCCCAGATCTCGGTCTCGATTTCAATGGAGGCGGCGTCGGAGGGAGAAAATCAGAACAAAAATCATGAGAGCAATAGTAAACTAAACAATTCCAGTGAGGGGCAGAGCAAGCCCAAGCGCCAGATGAAGACCCCCTTTCAGCTCGAAACCCTCGAGAAAGCATATGCATTGGAGACCTACCCGTCGGAGGCGATTAGGGCTGAGCTATCGGAAAAATTGGGGCTTTTCGATCGGCAGTTGCAGATGTGGTTCTGTCACAGAAGGTTGAAGGACAAGAAGGAAGGGGGTCCTCCGAAGAAGCAGCGGAAATTGGTACCCACTTTACCTGAGCCCCCCATTGATGATTTGGCTCATGGGTCAGAGCCGGGTAGTGATTATGGGTCCGGTTCAGGCTCCGGCTCAAGCCCCTTTGGTCACCCGGAGTTGCGGAATGTGGTGGCTAGGGGTGTGGCTGATGACATTCCAATGAGGGGGAGGAGGTATTATGAGTCGCCACAGTCGATCTTGGAGCTCAGGGCCATTGCTTGTGTGGAAGCTCAATTGGGAGAGCCATTGAGGGAAGATGGCCCGCTTCTCGGGATAGAGTTTGATCCTTTGCCGCCAGATGCATTTGGGGCACCTCTAGTTGCGGAGCAGCATAAGCGGTCTGCCCATGCTTTGGAGGGCAAATATGAGCGTCATGATGCAAAACCAATTAAAGCTACGACAAGGGCTCTCCACGAATATCCCTTTCTTCAAGATCACTCCAGTATTCGGTCTGATGCATACGGACAAGTCTCTCAATCTCATTTTCACGATTCAGCAATTGATGGCCCAACTGCAAGAACTTCGTCATTTGCTGTTGGAAATGAGCCATTGTCAAGGGTTCATGGTGTCCATGGTCATGCGTCACGTGTTCGTCTTCTGTCTCAGCAGGAGAGGCAAGTAGTGCCCTACTCATCTCCTGTTGATGATGGCAGTGTAGCACAAAGGGACTCCTTCACTAACGTGAGGGTAAATACTCAGTTTAGTGATCCCCCTGTTGTTGCACCTGCACTGGAAAACTCTAACGTATTATCTGAAGGTCAAATTAATGACTCTATTCTGAGAATGGAGAGGAAACGCAAGAGTGAAGAAGCGAGAATCGCTAAAGAAGTTGAAGCTCATGAGTTGCGGATCCGGAAGGAGCTGGAGAAACAAGATATTCTGAGGAGAAAGAATGAGGAACGAATGAGGAAGGAAATGGAAAAACAAGACCGTGAGAGACGAAAAGAAGAAGAGAGGTTGATGCGTGAGAGGCAACGCGAGGAAGAGAGATTAAAGCGTGAGCAAAAACGTGAAATTGAACGAAGGGAAAAGTTTTTACAGAAAGAATATATAAGAGCTGAGAAAATGAGGCAGAAGGAAGAGCTTCGCAAAGAGAGAGAGGTAGTGAAACGCAAAGCTGCACTTGAGAAGGCGACTGCACGTAGGCTTGCTAAAGAGTCTATGGAGCTTATTGAGGATGAACAACTAGAATTAATGGAGTTGGCTGCTACAAGCAAGGGTTTATCCTCCATTATTTGTATTGATCTTGACACCCTGCAAAACCTTGATGCATTCAGAGATTCTTTGGTTGCATTCCCACCTAGGTCTGTGCAGTTAAAAGGACCATTTGCAATTCAGCCATGGATCAATTCAGAGGCGAATATTGGTAACCTTCTCATGGTTTGGAGATTCTTAATTACCTTTGCTGATGTTCTCGAGTTATGGCCTTTTACTCTTGATGAGTTTGTTCAAGCATTTCATGACTATGATTCAAGGTTGTTAGGAGAGATCCATGTTGCTCTCTTGAGGTTGGTTATTAAAGATATTGAGGATGTTGCACGGACTCCTACTGGATTAGGAATAAATCAAAATGGTGCTGCTAATCCTGGAGGTGGACACCCACAGATTGTTGAAGGAGCTTATGCATGGGGCTTTGATATACGGAACTGGCAGCAGCACTTAAATCTGCTGACATGGCCTGAAATATTCCGTCAACTAGCACTCTCTGCCGGATTTGGGCCACAGCTGAAGAAAAGGAGTATCGCATGGTCATACACACCTGATAATCACGAGGGTAAAGGTTGTCAGGATGCAATTTCTAATCTACGAAATGGTTCTGCAGCTGTAAATGCATTTGCAGTAATGCAAGAGAAAGGCTTACTGGCTCCACGAAGATCTAGACATCGGTTGACTCCTGGAACTGTAAAGTTTGCAGCTTTTCATGTGCTTTCACTTGAGGGAAATAAGGGACTAACAGTGATCGATCTTGCAGACAAGATTCAGAAATCCGGCCTTCGGGACCTGACAACAAGCAAGACTCCTGAAGCGTCAATTTCTGTTGCTTTGACAAGAGATACTAAGCTTTTTGAAAGAATAGCTCCTTCAACATATCGTGTACGAGCTGCTTACAGAAACGATCCTGTTGATGCCGAGGCCATACTTTCTGCAGCCAGGAAGAAAATTCAGATATTTGAAAATGGGATTTTAGCTGCAGAAGATGTCGATGATGTTGAAAGAGATGATGTTGAAAATGATGAGGTTGAAAGAGACGAAGAATCTGAATGTGAAGTTGATGAAGATCCTGAAGTTGATGACTTAGCTACTCCATCAGTTGCAAACAAATCCCCTGATGATTACAATGAAGTAATTAGTTTCTCAGAAAATGGAAAGGGCAGTTTAAGCAATGATGTTGGACTTGCTGTGAAAAATGAGTTTGATAAGGATGTTTTATGTTCCCCAGTGACTGGTTCCAAAGATGCATTCTGTCCTAGTGCTTCCTTAAAACAGTGTGTTTTGGGTGCAGATATTAGTACTAGTCATCTTGACCAAGAAAATATGGAGATTGATGAAAGCAAGTCAGGTGAGTCATGGGTTCAAGGACTTACAGAAGGGGAGTATTCTGACCTCAGCGTGGAAGAGCGTCTTAGTGGTCTTGTTGCCTTAATTGGTGTTGCAAATGAAGGAAATACCATTCGTATTGTTCTCGAGGATCGCTTAGAAGCTGCAAATGCTCTTAAGAAGCAAATGTGGGCAGAAGCACAGCTAGATAAAAGTCGCCTAAAAGAAGAGAATGTTGGTAGGTTAGATATTCCATCTTTAATGGGAGGCAAAGCTGAAGTACAAGCTACCGGAGTAGAGGACGGCCTAAGTCCCTTACGGGATGTTGATAACAGGAACATTGATGCATCTCCAGTCACTGCAGAAAATGAAAAGTCAATCCATGGGTCACAAGGTGTTCAGAATCAGCTTAATGGCGTGCATGTTGAAAGGACCTCAGCAGCCCAAGATATCTCCATGGGTTCTGATAACTTTTTGACTCAGCAACTTGCATATGCATCAAAAAGGTCGCGCTCACAATTAAAATCGTATATTGCACATAGAGCAGAAGAGATGTATGCATACAGGTCCCTGCCTCTTGGTCAAGATCGTAGGCATAATCGATACTGGCAATTTGTTGCATCTGCATCAAGACATGACCCTGGTTCAGGTAGAATTTTCATTGAGTTGAACAATGGAAGCTGGAGGCTTATTGACACTGAAGAGGCCTTTGATGCTCTTGTTACGTCTCTGGACACACGTGGAATTAGGGAATCTCATTTACGCTTAATGTTGCAAAAGATTGAGGCATCCTTCAAGGAGAACGTTCAGAAAAATACGCATCGTCCTAACAATGCAGGCCCAACTAAAAACCATGTTAAAAATGAAGCTGACGAAATGGATTCTAGTCCTGATTTCCCCTCTCATTTTGACAGCCCTGAAAGTGCAGTTTGTGCTTTGAATTCTGATGCAGCACAGACCTCATCTTCTTTCAGAATTGAGCTTGACAGTAGTGAAACTGAGAAAAGGGCTGCCTTGAGGAGGTATCAAGATTTTCAGAAGTGGATGTGGAAAGAATGCTCTAATTCATCTACCTTATGTGCCACGAAATATGGGAAGGAGAGATGCAGACCGCTGTTTGATGTCTGCGATGTCTGCCTTAATTGTTATTACTTTGAGGATTTCCACTGCGATACTTGCCATCAGACTTTTGATGCTTTCAACAGAAATTTTGACTTTGGTGAACATGTAATGCAATGTAAAGAAAAAAGGATGTTGGAACCTTGGAATTTCGATATTCCTTGTACTTCTCTTCCCTTGGCCAGAAGATTGCTCAAGACATTAATAGCTCTTGTTGAGGTATCTATTCCACCAGAAGCTCTTCATTTGTCTTGGACTGATGACCATCGAAAGATGTGGGGAGAGAAACTGAATGCATCATCATCAATGGATGAACTTCTTCAGATATTGACTCTGGTTGAGACTGCAGTGAAGAAAGACTTTCTGTCATCACACTTTGCAATGACGGAGGAATTGCTTGGCTCCTGCAAACAGTCGGGGGTTGTTCGCGATTATCTAGACAGTGGATCCGTTCCTGTACTTCCTTGGATACCACATACAACTGCAGCTGTGGCTCTGAGGCTTCATGAGATAGATTCATCAATTACATATGTAAAGCAAGCGAAAGCTGAGCCCACCGCGGACAAGGAAGTGGGAGAATATCTCAATCAACCTATGAGACTTCCGACATCGAGGAGTGAGACTGAACTAACTGAAGCAGGCCTCAATGAATACATGAGGGAAGAAAACAGTACTCACCTGAAAAGTGGACGAAACAGCTTTAGACGTGGTAGAGGAGGTCGAGAGCAAGGACGTGGTAAAAGGTGGCAGAAAAAAGTGAGTAATCCCAAATCTGGTGCAAGTCGTCAGAACATTAGGGGTAATGAGAATTTGAATCAAGGGTTGAGAACGGTGGGAAAAAGAACGCAGGGACAAGGGAGCGGACGGGGTCGCCGAACTGTTAGAAAGAGGAGAATGAAGAACAGGGCTGTTGAGGGGACACCTCTTGCCCATGTGAGTGACATGCGCAGCAGCCCCGAGAGTGGAGGAGAATCACCAAGAAACTTGGCTGAGGAATGGGATGATGAAAAGATCGATATGAGAGGTGACGAGCAAGGAGGGGGATTTGAACGAGTAGACACATTGGAATCAGAAGACGACGACCAAGCAGTGGGATACGAACAAGGAAGCTGGGAAATTGGATTTGATGGGACTTCCAGCGGATGGAACGGGGGTTTAGTAGAAGCTAGTGACGATGATATAGATGCTTCAGAAGATGACAATAATGTCATGGAAGAAGTGAGAGATGAAGATTCAGAAGGGGATGTAGATATGAGCGATGCCTCGGACGAAATTCTGCCAAACACGATGAGAAACGATGATGGCACGGAGTCGGAAAGTTCGGATGACTCTAGTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000122 GO:0000976 GO:0000977 GO:0000978 GO:0000981 GO:0000982 GO:0000987 GO:0001012 GO:0001067 GO:0001077 GO:0001227 GO:0001228 GO:0001525 GO:0001568 GO:0001944 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009791 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010228 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0030154 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0032501 GO:0032502 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0035239 GO:0035295 GO:0040034 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048506 GO:0048509 GO:0048510 GO:0048514 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0072358 GO:0072359 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1990837 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT HARE-HTH Homeobox WHIM1 WSD

Protein Analysis

1885

Amino Acids

212.56

Weight (kDa)

5.47

Isoelectric Point (pI)

48.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 154 - 210 1.4e-15 Homeodomain
DDT PF02791 673 - 727 1e-19 DDT domain
HARE-HTH PF05066 852 - 920 1.5e-17 HB1, ASXL, restriction endonuclease HTH domain
WHIM1 PF15612 1071 - 1115 2.8e-07 WSTF, HB1, Itc1p, MBD9 motif 1
WSD PF15613 1253 - 1326 4.5e-22 Williams-Beuren syndrome DDT (WSD), D-TOX E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011708)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28420
fragaria_vesca FvH4_3g08620
malus_domestica MD05G1310200.v1.1 MD10G1289100.v1.1
prunus_persica Prupe.4G052600_v2.0.a1
pyrus_communis pycom05g28660 pycom10g24170
rosa_chinensis RchiOBHm_Chr5g0009221
rosa_laevigata RLG00000031635 RLG00000031641
rosa_multiflora Rmu_sc0006233.1_g000030
rosa_roxburghii Rroxscaffold_1G00066840 Rroxscaffold_1G00067540
rosa_rugosa Rorug04G0441600
rosa_samantha Rh5AG071400 Rh5BG067900 Rh5CG080200 Rh5DG067900
rosa_wichuraiana Rw5G006670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1390
AasI GACNNNNNNGTC 2 cut(s) 532, 5519
Acc16I TGCGCA 1 cut(s) 5261
Acc36I ACCTGC 1 cut(s) 1390
Acc65I GGTACC 1 cut(s) 664
AccB1I GGYRCC 2 cut(s) 664, 967
AccB7I CCANNNNNTGG 4 cut(s) 1009, 3101, 5404, 5457
AccBSI CCGCTC 1 cut(s) 5175
AccI GTMKAC 1 cut(s) 5370
AccII CGCG 4 cut(s) 1635, 3706, 4743, 4874
AccIII TCCGGA 1 cut(s) 1509
AclI AACGTT 1 cut(s) 3999
AcoI YGGCCR 1 cut(s) 4494
AcsI RAATTY 8 cut(s) 178, 2817, 3849, 4135, 4402, 4462, 5122, 5586
AcuI CTGAAG 9 cut(s) 1431, 2405, 2700, 2961, 3492, 3915, 4619, 4968, 5487
AcvI CACGTG 2 cut(s) 1244, 3935
AcyI GRCGYC 1 cut(s) 386
AdeI CACNNNGTG 1 cut(s) 3590
AfaI GTAC 7 cut(s) 666, 2754, 3169, 3477, 4480, 4773, 4987
AflII CTTAAG 1 cut(s) 3371
AflIII ACRYGT 4 cut(s) 61, 1243, 3932, 4420
AhdI GACNNNNNGTC 1 cut(s) 4678
AhlI ACTAGT 1 cut(s) 3170
AjiI CACGTC 2 cut(s) 5021, 5048
AjnI CCWGG 4 cut(s) 2242, 2551, 2807, 3835
AjuI GAANNNNNNNTTGG 4 cut(s) 3101, 3133, 3414, 3446
AleI CACNNNNGTG 1 cut(s) 1337
Alw21I GWGCWC 1 cut(s) 864
AlwNI CAGNNNCTG 2 cut(s) 3101, 3554
Ama87I CYCGRG 5 cut(s) 503, 926, 2072, 3341, 5270
Aor13HI TCCGGA 1 cut(s) 1509
ApoI RAATTY 8 cut(s) 178, 2817, 3849, 4135, 4402, 4462, 5122, 5586
ArsI GACNNNNNNTTYG 2 cut(s) 324, 356
AseI ATTAAT 4 cut(s) 107, 1422, 1850, 4517
Asp700I GAANNNNTTC 4 cut(s) 1740, 2470, 4554, 4632
Asp718I GGTACC 1 cut(s) 664
AspA2I CCTAGG 1 cut(s) 1958
AspLEI GCGC 3 cut(s) 471, 3708, 5262
AsuC2I CCSGG 2 cut(s) 717, 770
AsuHPI GGTGA 6 cut(s) 758, 3230, 4427, 4982, 5280, 5351
AvaI CYCGRG 5 cut(s) 503, 926, 2072, 3341, 5270
AvaII GGWCC 6 cut(s) 638, 732, 1979, 2656, 3627, 3762
AvrII CCTAGG 1 cut(s) 1958
BaeGI GKGCMC 2 cut(s) 970, 1284
BalI TGGCCA 1 cut(s) 4496
BamHI GGATCC 2 cut(s) 1506, 4760
BanI GGYRCC 2 cut(s) 664, 967
BanII GRGCYC 4 cut(s) 684, 864, 1066, 4869
BauI CACGAG 1 cut(s) 2426
BbrPI CACGTG 2 cut(s) 1244, 3935
BbsI GAAGAC 3 cut(s) 485, 1244, 5394
Bbv12I GWGCWC 1 cut(s) 864
BbvCI CCTCAGC 3 cut(s) 3263, 3631, 5304
BceAI ACGGC 1 cut(s) 3514
BcgI CGANNNNNNTGC 2 cut(s) 1161, 1195
BciT130I CCWGG 4 cut(s) 2244, 2553, 2809, 3837
BciVI GTATCC 2 cut(s) 4776, 5402
BcnI CCSGG 2 cut(s) 717, 770
BcuI ACTAGT 1 cut(s) 3170
BfmI CTRYAG 6 cut(s) 2475, 2802, 2847, 3555, 4659, 4797
BfoI RGCGCY 1 cut(s) 472
BfrI CTTAAG 1 cut(s) 3371
BfuAI ACCTGC 1 cut(s) 1390
BfuI GTATCC 2 cut(s) 4776, 5402
BglII AGATCT 2 cut(s) 357, 2531
BlnI CCTAGG 1 cut(s) 1958
BlpI GCTNAGC 2 cut(s) 552, 4863
BmcAI AGTACT 2 cut(s) 3169, 4987
Bme1390I CCNGG 6 cut(s) 717, 770, 2244, 2553, 2809, 3837
Bme18I GGWCC 6 cut(s) 638, 732, 1979, 2656, 3627, 3762
BmeRI GACNNNNNGTC 1 cut(s) 4678
BmeT110I CYCGRG 5 cut(s) 503, 926, 2072, 3341, 5270
BmgBI CACGTC 2 cut(s) 5021, 5048
BmrFI CCNGG 6 cut(s) 717, 770, 2244, 2553, 2809, 3837
BmrI ACTGGG 1 cut(s) 3089
BmuI ACTGGG 1 cut(s) 3089
BpiI GAAGAC 3 cut(s) 485, 1244, 5394
BplI GAGNNNNNCTC 2 cut(s) 4881, 4913
BpmI CTGGAG 5 cut(s) 1081, 1541, 2265, 3531, 3898
Bpu10I CCTNAGC 5 cut(s) 678, 863, 3263, 3631, 5304
Bpu1102I GCTNAGC 2 cut(s) 552, 4863
BpuEI CTTGAG 6 cut(s) 736, 1802, 2182, 2612, 4231, 4493
BpuMI CCSGG 2 cut(s) 717, 770
Bsa29I ATCGAT 3 cut(s) 2619, 3796, 5328
BsaAI YACGTR 3 cut(s) 1244, 1799, 3935
BsaBI GATNNNNATC 2 cut(s) 2934, 4578
BsaHI GRCGYC 1 cut(s) 386
BsaI GGTCTC 2 cut(s) 370, 518
BsaWI WCCGGW 3 cut(s) 734, 1509, 3485
BsaXI ACNNNNNCTCC 2 cut(s) 371, 401
Bse3DI GCAATG 4 cut(s) 870, 3046, 4436, 4701
Bse8I GATNNNNATC 2 cut(s) 2934, 4578
BseAI TCCGGA 1 cut(s) 1509
BseBI CCWGG 4 cut(s) 2244, 2553, 2809, 3837
BseCI ATCGAT 3 cut(s) 2619, 3796, 5328
BseJI GATNNNNATC 2 cut(s) 2934, 4578
BseMI GCAATG 4 cut(s) 870, 3046, 4436, 4701
BseRI GAGGAG 8 cut(s) 46, 841, 1555, 4228, 4942, 5043, 5218, 5295
BseSI GKGCMC 2 cut(s) 970, 1284
BseYI CCCAGC 1 cut(s) 5424
BsgI GTGCAG 6 cut(s) 1368, 1761, 1779, 1988, 3177, 4140
Bsh1236I CGCG 4 cut(s) 1635, 3706, 4743, 4874
Bsh1285I CGRYCG 1 cut(s) 583
BshNI GGYRCC 2 cut(s) 664, 967
BshVI ATCGAT 3 cut(s) 2619, 3796, 5328
BsiEI CGRYCG 1 cut(s) 583
BsiHKAI GWGCWC 1 cut(s) 864
BsiHKCI CYCGRG 5 cut(s) 503, 926, 2072, 3341, 5270
BsiSI CCGG 8 cut(s) 716, 735, 747, 770, 1510, 2367, 2646, 3486
BslFI GGGAC 8 cut(s) 1337, 2619, 2669, 3492, 3748, 5178, 5245, 5458
BsmBI CGTCTC 3 cut(s) 1588, 2898, 3927
BsmFI GGGAC 8 cut(s) 1337, 2619, 2669, 3492, 3748, 5178, 5245, 5458
BsmI GAATGC 5 cut(s) 1928, 1949, 3116, 4256, 4612
Bso31I GGTCTC 2 cut(s) 370, 518
BsoBI CYCGRG 5 cut(s) 503, 926, 2072, 3341, 5270
Bsp1286I GDGCHC 6 cut(s) 684, 864, 970, 1066, 1284, 4869
Bsp13I TCCGGA 1 cut(s) 1509
Bsp1720I GCTNAGC 2 cut(s) 552, 4863
Bsp19I CCATGG 4 cut(s) 1228, 1997, 3577, 3651
Bsp68I TCGCGA 1 cut(s) 4743
BspDI ATCGAT 3 cut(s) 2619, 3796, 5328
BspEI TCCGGA 1 cut(s) 1509
BspFNI CGCG 4 cut(s) 1635, 3706, 4743, 4874
BspHI TCATGA 6 cut(s) 232, 415, 1030, 1495, 2116, 4819
BspMAI CTGCAG 6 cut(s) 2479, 2806, 2851, 3559, 4663, 4801
BspMI ACCTGC 1 cut(s) 1390
BspQI GCTCTTC 3 cut(s) 1731, 3267, 4560
BspT107I GGYRCC 2 cut(s) 664, 967
BspTI CTTAAG 1 cut(s) 3371
BspTNI GGTCTC 2 cut(s) 370, 518
BsrBI CCGCTC 1 cut(s) 5175
BsrDI GCAATG 4 cut(s) 870, 3046, 4436, 4701
BssNI GRCGYC 1 cut(s) 386
BssSI CACGAG 1 cut(s) 2426
BssT1I CCWWGG 8 cut(s) 1228, 1958, 1997, 3577, 3651, 4457, 4491, 4778
Bst2BI CACGAG 1 cut(s) 2426
Bst2UI CCWGG 4 cut(s) 2244, 2553, 2809, 3837
BstACI GRCGYC 1 cut(s) 386
BstAFI CTTAAG 1 cut(s) 3371
BstAPI GCANNNNNTGC 3 cut(s) 1279, 2363, 4127
BstBAI YACGTR 3 cut(s) 1244, 1799, 3935
BstC8I GCNNGC 7 cut(s) 461, 920, 1806, 3614, 4033, 4854, 4954
BstDSI CCRYGG 5 cut(s) 1228, 1997, 3577, 3651, 4872
BstEII GGTNACC 2 cut(s) 764, 2024
BstENI CCTNNNNNAGG 2 cut(s) 3840, 4213
BstFNI CGCG 4 cut(s) 1635, 3706, 4743, 4874
BstH2I RGCGCY 1 cut(s) 472
BstHHI GCGC 3 cut(s) 471, 3708, 5262
BstMCI CGRYCG 1 cut(s) 583
BstNI CCWGG 4 cut(s) 2244, 2553, 2809, 3837
BstNSI RCATGY 4 cut(s) 65, 3620, 4424, 5260
BstPI GGTNACC 2 cut(s) 764, 2024
BstSCI CCNGG 6 cut(s) 715, 768, 2242, 2551, 2807, 3835
BstSFI CTRYAG 6 cut(s) 2475, 2802, 2847, 3555, 4659, 4797
BstSLI GKGCMC 2 cut(s) 970, 1284
BstUI CGCG 4 cut(s) 1635, 3706, 4743, 4874
BstV2I GAAGAC 3 cut(s) 485, 1244, 5394
BstX2I RGATCY 6 cut(s) 357, 1506, 2145, 2531, 2935, 4760
BstYI RGATCY 6 cut(s) 357, 1506, 2145, 2531, 2935, 4760
Bsu15I ATCGAT 3 cut(s) 2619, 3796, 5328
BsuI GTATCC 2 cut(s) 4776, 5402
BsuTUI ATCGAT 3 cut(s) 2619, 3796, 5328
BtgI CCRYGG 5 cut(s) 1228, 1997, 3577, 3651, 4872
BtgZI GCGATG 2 cut(s) 4338, 5586
BtrI CACGTC 2 cut(s) 5021, 5048
BtsI GCAGTG 5 cut(s) 1315, 3552, 4360, 4668, 5409
BtuMI TCGCGA 1 cut(s) 4743
BveI ACCTGC 1 cut(s) 1390
Cac8I GCNNGC 7 cut(s) 461, 920, 1806, 3614, 4033, 4854, 4954
CaiI CAGNNNCTG 2 cut(s) 3101, 3554
CciI TCATGA 6 cut(s) 232, 415, 1030, 1495, 2116, 4819
CfoI GCGC 3 cut(s) 471, 3708, 5262
Cfr42I CCGCGG 1 cut(s) 4875
ClaI ATCGAT 3 cut(s) 2619, 3796, 5328
CseI GACGC 5 cut(s) 375, 1016, 1227, 2673, 3266
Csp6I GTAC 7 cut(s) 665, 2753, 3168, 3476, 4479, 4772, 4986
CviQI GTAC 7 cut(s) 665, 2753, 3168, 3476, 4479, 4772, 4986
DraI TTTAAA 1 cut(s) 55
DraIII CACNNNGTG 1 cut(s) 3590
DrdI GACNNNNNNGTC 2 cut(s) 532, 5519
DriI GACNNNNNGTC 1 cut(s) 4678
DseDI GACNNNNNNGTC 2 cut(s) 532, 5519
EaeI YGGCCR 1 cut(s) 4494
Eam1105I GACNNNNNGTC 1 cut(s) 4678
Ecl136II GAGCTC 1 cut(s) 862
Eco130I CCWWGG 8 cut(s) 1228, 1958, 1997, 3577, 3651, 4457, 4491, 4778
Eco147I AGGCCT 2 cut(s) 3902, 4956
Eco24I GRGCYC 4 cut(s) 684, 864, 1066, 4869
Eco31I GGTCTC 2 cut(s) 370, 518
Eco32I GATATC 1 cut(s) 3646
Eco47I GGWCC 6 cut(s) 638, 732, 1979, 2656, 3627, 3762
Eco53kI GAGCTC 1 cut(s) 862
Eco57I CTGAAG 9 cut(s) 1431, 2405, 2700, 2961, 3492, 3915, 4619, 4968, 5487
Eco72I CACGTG 2 cut(s) 1244, 3935
Eco88I CYCGRG 5 cut(s) 503, 926, 2072, 3341, 5270
Eco91I GGTNACC 2 cut(s) 764, 2024
EcoICRI GAGCTC 1 cut(s) 862
EcoNI CCTNNNNNAGG 2 cut(s) 3840, 4213
EcoO109I RGGNCCY 4 cut(s) 638, 2656, 3627, 3762
EcoO65I GGTNACC 2 cut(s) 764, 2024
EcoRI GAATTC 1 cut(s) 4135
EcoRII CCWGG 4 cut(s) 2242, 2551, 2807, 3835
EcoRV GATATC 1 cut(s) 3646
EcoT14I CCWWGG 8 cut(s) 1228, 1958, 1997, 3577, 3651, 4457, 4491, 4778
EcoT38I GRGCYC 4 cut(s) 684, 864, 1066, 4869
ErhI CCWWGG 8 cut(s) 1228, 1958, 1997, 3577, 3651, 4457, 4491, 4778
Esp3I CGTCTC 3 cut(s) 1588, 2898, 3927
FalI AAGNNNNNCTT 8 cut(s) 470, 502, 1764, 1796, 3264, 3296, 4658, 4690
FaqI GGGAC 8 cut(s) 1337, 2619, 2669, 3492, 3748, 5178, 5245, 5458
FauI CCCGC 1 cut(s) 927
FauNDI CATATG 3 cut(s) 514, 3688, 4843
FblI GTMKAC 1 cut(s) 5370
FriOI GRGCYC 4 cut(s) 684, 864, 1066, 4869
FspI TGCGCA 1 cut(s) 5261
GlaI GCGC 3 cut(s) 470, 3707, 5261
GsaI CCCAGC 1 cut(s) 5428
GsuI CTGGAG 5 cut(s) 1081, 1541, 2265, 3531, 3898
HaeII RGCGCY 1 cut(s) 472
HapII CCGG 8 cut(s) 716, 735, 747, 770, 1510, 2367, 2646, 3486
HgaI GACGC 5 cut(s) 375, 1016, 1227, 2673, 3266
HhaI GCGC 3 cut(s) 471, 3708, 5262
Hin1I GRCGYC 1 cut(s) 386
Hin6I GCGC 3 cut(s) 469, 3706, 5260
HinP1I GCGC 3 cut(s) 469, 3706, 5260
HincII GTYRAC 2 cut(s) 2351, 2547
HindII GTYRAC 2 cut(s) 2351, 2547
HindIII AAGCTT 1 cut(s) 2717
HpaII CCGG 8 cut(s) 716, 735, 747, 770, 1510, 2367, 2646, 3486
HphI GGTGA 6 cut(s) 758, 3230, 4427, 4982, 5280, 5351
Hpy166II GTNNAC 8 cut(s) 431, 2252, 2351, 2547, 2753, 4418, 5003, 5371
Hpy8I GTNNAC 8 cut(s) 431, 2252, 2351, 2547, 2753, 4418, 5003, 5371
Hpy99I CGWCG 3 cut(s) 391, 538, 5396
Hsp92I GRCGYC 1 cut(s) 386
HspAI GCGC 3 cut(s) 469, 3706, 5260
Kpn2I TCCGGA 1 cut(s) 1509
KpnI GGTACC 1 cut(s) 668
KspI CCGCGG 1 cut(s) 4875
LguI GCTCTTC 3 cut(s) 1731, 3267, 4560
MbiI CCGCTC 1 cut(s) 5175
MfeI CAATTG 2 cut(s) 890, 1155
MflI RGATCY 6 cut(s) 357, 1506, 2145, 2531, 2935, 4760
MhlI GDGCHC 6 cut(s) 684, 864, 970, 1066, 1284, 4869
MlsI TGGCCA 1 cut(s) 4496
MluNI TGGCCA 1 cut(s) 4496
MmeI TCCRAC 7 cut(s) 369, 516, 1174, 3028, 4431, 4943, 5607
Mox20I TGGCCA 1 cut(s) 4496
MroI TCCGGA 1 cut(s) 1509
MroXI GAANNNNTTC 4 cut(s) 1740, 2470, 4554, 4632
MscI TGGCCA 1 cut(s) 4496
MslI CAYNNNNRTG 5 cut(s) 815, 1233, 1337, 2121, 5250
Msp20I TGGCCA 1 cut(s) 4496
MspA1I CMGCKG 7 cut(s) 655, 2383, 2480, 4309, 4802, 4874, 5454
MspCI CTTAAG 1 cut(s) 3371
MspI CCGG 8 cut(s) 716, 735, 747, 770, 1510, 2367, 2646, 3486
MspR9I CCNGG 6 cut(s) 717, 770, 2244, 2553, 2809, 3837
MunI CAATTG 2 cut(s) 890, 1155
Mva1269I GAATGC 5 cut(s) 1928, 1949, 3116, 4256, 4612
MvaI CCWGG 4 cut(s) 2244, 2553, 2809, 3837
MvnI CGCG 4 cut(s) 1635, 3706, 4743, 4874
NciI CCSGG 2 cut(s) 717, 770
NcoI CCATGG 4 cut(s) 1228, 1997, 3577, 3651
NdeI CATATG 3 cut(s) 514, 3688, 4843
NmeAIII GCCGAG 1 cut(s) 2812
NmuCI GTSAC 9 cut(s) 605, 764, 1239, 3097, 3550, 3582, 5252, 5339, 5483
NruI TCGCGA 1 cut(s) 4743
NsbI TGCGCA 1 cut(s) 5261
NspI RCATGY 4 cut(s) 65, 3620, 4424, 5260
OliI CACNNNNGTG 1 cut(s) 1337
PaeR7I CTCGAG 3 cut(s) 503, 2072, 3341
PagI TCATGA 6 cut(s) 232, 415, 1030, 1495, 2116, 4819
PaqCI CACCTGC 1 cut(s) 1390
PceI AGGCCT 2 cut(s) 3902, 4956
PciI ACATGT 2 cut(s) 61, 4420
PciSI GCTCTTC 3 cut(s) 1731, 3267, 4560
PcsI WCGNNNNNNNCGW 3 cut(s) 501, 848, 5185
PctI GAATGC 5 cut(s) 1928, 1949, 3116, 4256, 4612
PdmI GAANNNNTTC 4 cut(s) 1740, 2470, 4554, 4632
PflFI GACNNNGTC 1 cut(s) 5181
PflMI CCANNNNNTGG 4 cut(s) 1009, 3101, 5404, 5457
PfoI TCCNGGA 2 cut(s) 2242, 2551
Ple19I CGATCG 1 cut(s) 583
PmaCI CACGTG 2 cut(s) 1244, 3935
PmlI CACGTG 2 cut(s) 1244, 3935
Ppu21I YACGTR 3 cut(s) 1244, 1799, 3935
PpuMI RGGWCCY 4 cut(s) 638, 2656, 3627, 3762
PscI ACATGT 2 cut(s) 61, 4420
PshBI ATTAAT 4 cut(s) 107, 1422, 1850, 4517
Psp124BI GAGCTC 1 cut(s) 864
Psp1406I AACGTT 1 cut(s) 3999
Psp5II RGGWCCY 4 cut(s) 638, 2656, 3627, 3762
Psp6I CCWGG 4 cut(s) 2242, 2551, 2807, 3835
PspCI CACGTG 2 cut(s) 1244, 3935
PspEI GGTNACC 2 cut(s) 764, 2024
PspFI CCCAGC 1 cut(s) 5424
PspGI CCWGG 4 cut(s) 2242, 2551, 2807, 3835
PspPPI RGGWCCY 4 cut(s) 638, 2656, 3627, 3762
PstI CTGCAG 6 cut(s) 2479, 2806, 2851, 3559, 4663, 4801
PstNI CAGNNNCTG 2 cut(s) 3101, 3554
PsuI RGATCY 6 cut(s) 357, 1506, 2145, 2531, 2935, 4760
PsyI GACNNNGTC 1 cut(s) 5181
PvuI CGATCG 1 cut(s) 583
PvuII CAGCTG 3 cut(s) 2383, 2480, 4802
RruI TCGCGA 1 cut(s) 4743
RsaI GTAC 7 cut(s) 666, 2754, 3169, 3477, 4480, 4773, 4987
RsaNI GTAC 7 cut(s) 665, 2753, 3168, 3476, 4479, 4772, 4986
RseI CAYNNNNRTG 5 cut(s) 815, 1233, 1337, 2121, 5250
SacI GAGCTC 1 cut(s) 864
SacII CCGCGG 1 cut(s) 4875
SapI GCTCTTC 3 cut(s) 1731, 3267, 4560
ScaI AGTACT 2 cut(s) 3169, 4987
ScrFI CCNGG 6 cut(s) 717, 770, 2244, 2553, 2809, 3837
SduI GDGCHC 6 cut(s) 684, 864, 970, 1066, 1284, 4869
SfcI CTRYAG 6 cut(s) 2475, 2802, 2847, 3555, 4659, 4797
Sfr274I CTCGAG 3 cut(s) 503, 2072, 3341
Sfr303I CCGCGG 1 cut(s) 4875
SgrBI CCGCGG 1 cut(s) 4875
SinI GGWCC 6 cut(s) 638, 732, 1979, 2656, 3627, 3762
SlaI CTCGAG 3 cut(s) 503, 2072, 3341
SmiMI CAYNNNNRTG 5 cut(s) 815, 1233, 1337, 2121, 5250
SpeI ACTAGT 1 cut(s) 3170
SseBI AGGCCT 2 cut(s) 3902, 4956
SspI AATATT 4 cut(s) 111, 225, 2020, 2343
SstI GAGCTC 1 cut(s) 864
StuI AGGCCT 2 cut(s) 3902, 4956
StyD4I CCNGG 6 cut(s) 715, 768, 2242, 2551, 2807, 3835
StyI CCWWGG 8 cut(s) 1228, 1958, 1997, 3577, 3651, 4457, 4491, 4778
TaqII GACCGA 2 cut(s) 352, 1095
TatI WGTACW 5 cut(s) 3167, 3475, 4478, 4771, 4985
TauI GCSGC 2 cut(s) 386, 952
TseFI GTSAC 9 cut(s) 605, 764, 1239, 3097, 3550, 3582, 5252, 5339, 5483
Tsp45I GTSAC 9 cut(s) 605, 764, 1239, 3097, 3550, 3582, 5252, 5339, 5483
TspGWI ACGGA 8 cut(s) 349, 1134, 2212, 2314, 2336, 4715, 4753, 5636
Tth111I GACNNNGTC 1 cut(s) 5181
Van91I CCANNNNNTGG 4 cut(s) 1009, 3101, 5404, 5457
Vha464I CTTAAG 1 cut(s) 3371
VpaK11BI GGWCC 6 cut(s) 638, 732, 1979, 2656, 3627, 3762
VspI ATTAAT 4 cut(s) 107, 1422, 1850, 4517
XagI CCTNNNNNAGG 2 cut(s) 3840, 4213
XapI RAATTY 8 cut(s) 178, 2817, 3849, 4135, 4402, 4462, 5122, 5586
XbaI TCTAGA 2 cut(s) 2534, 4750
XceI RCATGY 4 cut(s) 65, 3620, 4424, 5260
XcmI CCANNNNNNNNNTGG 2 cut(s) 694, 877
XhoI CTCGAG 3 cut(s) 503, 2072, 3341
XmaJI CCTAGG 1 cut(s) 1958
XmiI GTMKAC 1 cut(s) 5370
XmnI GAANNNNTTC 4 cut(s) 1740, 2470, 4554, 4632
ZrmI AGTACT 2 cut(s) 3169, 4987
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.