pycom05g29690

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0014354
Physical Location & Seq
Reverse (-)
30071310 .. 30072272
963 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g29690.1

Sequence Viewer

Length: 963 bp
ATGGGTTGCTGTTTGAGTACCACAGACGCCGGAAAGTCCTCCGCTTTCGATCCTCAGAAGCACCGCCATTCGTTGGCGGGAACTGAAGGGTCAAGATCTGACGCAGCCCCGGAGAGCAGAGCGCCGCCGCCAATAGACGAAGAGACAGTGAAAGAAGTACTTTCGGAGACACCGAAACCCAAACCTTCACCGCAGTCTTCGCCGCCGCCGCTATTCAAACACGAGCCAGTACTAGATCGAGATCAAGGAAAAATAGCAGCTTCCGAGGAAGAAGAGCTACCAGTTTTCGTTTCTTTGAAAACTAAATTCGACCCGGAAAAGATTGAGCAGAAAGTACCCATTTGCAACAACAATGACGGCGGCGGAGAGGTCTCGGAGTTGTCGGAGATTTGCAGCCTGAGCGAGAGCATATCGGGCACCACCGTTACCAGAGACGACGACGAAGAGGTTCATCAGAGGTTCGTCAACAGGTCGCCGGTGAAATTACGGCAAAACCGGGACTCTTCAAGTTCAATGGGTCAAAGGAGAGACCGAGTCGTGTGTAAGTCTCCGTCTCGGATAACCGAATCCTCTCCGGGTCGGAGATATGGGCCAAATGGGGCTGAGTCAGTGAGGTTGGTTCGGAGCAGAGAACCAAGCCCGAGTCAGCAGCCAATGTCCAGGCGTGGGTCGAGACCCGAGTCAAACCGTCGCGAACCAGGTGAGAGTTCGGGTCGTAGGTCAAGGTCACCGGCCACGAGGGTTACAGATGGCGGTGGGGTTAATAGGGCCAATGTGGGTCGGAGCCCGTCCGCCAGGAAATCCGGAAAGTATCCTGGTCGAACCACAATTGGCCCAATTGAGAGTTCGAGTTTTGGTCCGATCCGGAGAGTGGCGGAGGAGCCCAACAGCGGGGGTAACTGGCCCGCAAACGAGTCTCTCGACAACCCACATGTTTCCTTGGAGTGCTTTATTTTCCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

321

Amino Acids

34.81

Weight (kDa)

8.52

Isoelectric Point (pI)

89.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11125 AT1G61170
fragaria_vesca FvH4_3g04520
malus_domestica MD10G1300500.v1.1
prunus_persica Prupe.4G040000_v2.0.a1
pyrus_communis pycom05g29690 pycom10g25340
rosa_chinensis RchiOBHm_Chr5g0007441
rosa_laevigata RLG00000031457
rosa_multiflora Rmu_co8198018.1_g000001
rosa_roxburghii Rroxscaffold_1G00069030
rosa_rugosa Rorug04G0426900
rosa_samantha Rh5AG055300 Rh5BG052900 Rh5CG062700 Rh5DG051900
rosa_wichuraiana Rw5G005480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 416
AccB7I CCANNNNNTGG 1 cut(s) 73
AccII CGCG 1 cut(s) 693
AccIII TCCGGA 2 cut(s) 803, 864
AclWI GGATC 2 cut(s) 44, 856
AcoI YGGCCR 1 cut(s) 732
AcsI RAATTY 1 cut(s) 305
AcuI CTGAAG 1 cut(s) 105
AcyI GRCGYC 1 cut(s) 27
AfaI GTAC 4 cut(s) 19, 159, 231, 336
AfiI CCNNNNNNNGG 5 cut(s) 73, 666, 871, 890, 891
AflIII ACRYGT 1 cut(s) 931
AgsI TTSAA 4 cut(s) 217, 298, 507, 513
AjnI CCWGG 4 cut(s) 659, 697, 794, 814
AjuI GAANNNNNNNTTGG 2 cut(s) 829, 861
AluBI AGCT 2 cut(s) 260, 277
AluI AGCT 2 cut(s) 260, 277
Alw26I GTCTC 9 cut(s) 137, 161, 376, 426, 522, 552, 558, 667, 921
AlwI GGATC 2 cut(s) 44, 856
Ama87I CYCGRG 2 cut(s) 640, 677
Aor13HI TCCGGA 2 cut(s) 803, 864
AoxI GGCC 5 cut(s) 590, 732, 768, 832, 902
ApeKI GCWGC 4 cut(s) 104, 257, 393, 649
ApoI RAATTY 1 cut(s) 305
Asp700I GAANNNNTTC 1 cut(s) 447
AspLEI GCGC 1 cut(s) 124
AspS9I GGNCC 5 cut(s) 590, 768, 833, 857, 903
AsuC2I CCSGG 4 cut(s) 110, 314, 497, 576
AsuHPI GGTGA 4 cut(s) 180, 490, 713, 720
AvaI CYCGRG 2 cut(s) 640, 677
AvaII GGWCC 1 cut(s) 857
BaeGI GKGCMC 1 cut(s) 419
BanI GGYRCC 1 cut(s) 416
BanII GRGCYC 2 cut(s) 788, 885
BarI GAAGNNNNNNTAC 2 cut(s) 261, 293
BauI CACGAG 2 cut(s) 221, 736
BbsI GAAGAC 1 cut(s) 189
BbvI GCAGC 4 cut(s) 116, 269, 405, 661
BccI CCATC 1 cut(s) 743
BceAI ACGGC 2 cut(s) 373, 503
BciT130I CCWGG 4 cut(s) 661, 699, 796, 816
BciVI GTATCC 1 cut(s) 822
BcnI CCSGG 4 cut(s) 110, 314, 497, 576
BcoDI GTCTC 9 cut(s) 137, 161, 376, 426, 522, 552, 558, 667, 921
BfaI CTAG 1 cut(s) 233
BfoI RGCGCY 1 cut(s) 125
BfuI GTATCC 1 cut(s) 822
BglII AGATCT 1 cut(s) 95
BmcAI AGTACT 2 cut(s) 159, 231
Bme1390I CCNGG 8 cut(s) 110, 314, 497, 576, 661, 699, 796, 816
Bme18I GGWCC 1 cut(s) 857
BmeT110I CYCGRG 2 cut(s) 640, 677
BmgT120I GGNCC 5 cut(s) 590, 768, 833, 857, 903
BmiI GGNNCC 3 cut(s) 418, 785, 882
BmrFI CCNGG 8 cut(s) 110, 314, 497, 576, 661, 699, 796, 816
BoxI GACNNNNGTC 1 cut(s) 679
BpiI GAAGAC 1 cut(s) 189
Bpu10I CCTNAGC 1 cut(s) 398
BpuMI CCSGG 4 cut(s) 110, 314, 497, 576
BsaBI GATNNNNATC 1 cut(s) 240
BsaHI GRCGYC 1 cut(s) 27
BsaI GGTCTC 3 cut(s) 376, 522, 667
BsaJI CCNNGG 3 cut(s) 108, 264, 939
BsaWI WCCGGW 2 cut(s) 803, 864
Bsc4I CCNNNNNNNGG 5 cut(s) 73, 666, 871, 890, 891
Bse118I RCCGGY 2 cut(s) 475, 730
Bse1I ACTGG 3 cut(s) 227, 281, 905
Bse8I GATNNNNATC 1 cut(s) 240
BseAI TCCGGA 2 cut(s) 803, 864
BseBI CCWGG 4 cut(s) 661, 699, 796, 816
BseDI CCNNGG 3 cut(s) 108, 264, 939
BseJI GATNNNNATC 1 cut(s) 240
BseLI CCNNNNNNNGG 5 cut(s) 73, 666, 871, 890, 891
BseMII CTCAG 3 cut(s) 68, 389, 594
BseNI ACTGG 3 cut(s) 227, 281, 905
BseRI GAGGAG 1 cut(s) 893
BseSI GKGCMC 1 cut(s) 419
BseXI GCAGC 4 cut(s) 116, 269, 405, 661
Bsh1236I CGCG 1 cut(s) 693
BshFI GGCC 5 cut(s) 592, 734, 770, 834, 904
BshNI GGYRCC 1 cut(s) 416
BsiHKCI CYCGRG 2 cut(s) 640, 677
BsiSI CCGG 9 cut(s) 30, 110, 314, 476, 496, 575, 731, 804, 865
BslFI GGGAC 1 cut(s) 512
BslI CCNNNNNNNGG 5 cut(s) 73, 666, 871, 890, 891
BsmAI GTCTC 9 cut(s) 137, 161, 376, 426, 522, 552, 558, 667, 921
BsmBI CGTCTC 2 cut(s) 426, 558
BsmFI GGGAC 1 cut(s) 512
BsnI GGCC 5 cut(s) 592, 734, 770, 834, 904
Bso31I GGTCTC 3 cut(s) 376, 522, 667
BsoBI CYCGRG 2 cut(s) 640, 677
Bsp1286I GDGCHC 3 cut(s) 419, 788, 885
Bsp13I TCCGGA 2 cut(s) 803, 864
Bsp143I GATC 5 cut(s) 49, 95, 235, 241, 861
Bsp68I TCGCGA 1 cut(s) 693
BspANI GGCC 5 cut(s) 592, 734, 770, 834, 904
BspCNI CTCAG 3 cut(s) 67, 390, 595
BspEI TCCGGA 2 cut(s) 803, 864
BspFNI CGCG 1 cut(s) 693
BspLI GGNNCC 3 cut(s) 418, 785, 882
BspPI GGATC 2 cut(s) 44, 856
BspQI GCTCTTC 1 cut(s) 267
BspT107I GGYRCC 1 cut(s) 416
BspTNI GGTCTC 3 cut(s) 376, 522, 667
BsrFI RCCGGY 2 cut(s) 475, 730
BsrI ACTGG 3 cut(s) 227, 281, 905
BssAI RCCGGY 2 cut(s) 475, 730
BssECI CCNNGG 3 cut(s) 108, 264, 939
BssMI GATC 5 cut(s) 49, 95, 235, 241, 861
BssNI GRCGYC 1 cut(s) 27
BssSI CACGAG 2 cut(s) 221, 736
BssT1I CCWWGG 1 cut(s) 939
Bst2BI CACGAG 2 cut(s) 221, 736
Bst2UI CCWGG 4 cut(s) 661, 699, 796, 816
Bst4CI ACNGT 3 cut(s) 148, 424, 689
Bst6I CTCTTC 4 cut(s) 135, 267, 438, 508
BstACI GRCGYC 1 cut(s) 27
BstC8I GCNNGC 1 cut(s) 906
BstDEI CTNAG 3 cut(s) 54, 398, 603
BstEII GGTNACC 1 cut(s) 726
BstFNI CGCG 1 cut(s) 693
BstH2I RGCGCY 1 cut(s) 125
BstHHI GCGC 1 cut(s) 124
BstKTI GATC 5 cut(s) 52, 98, 238, 244, 864
BstMAI GTCTC 9 cut(s) 137, 161, 376, 426, 522, 552, 558, 667, 921
BstMBI GATC 5 cut(s) 49, 95, 235, 241, 861
BstMWI GCNNNNNNNGC 4 cut(s) 199, 208, 399, 414
BstNI CCWGG 4 cut(s) 661, 699, 796, 816
BstNSI RCATGY 1 cut(s) 935
BstPAI GACNNNNGTC 1 cut(s) 679
BstPI GGTNACC 1 cut(s) 726
BstSCI CCNGG 8 cut(s) 108, 312, 495, 574, 659, 697, 794, 814
BstSLI GKGCMC 1 cut(s) 419
BstUI CGCG 1 cut(s) 693
BstV1I GCAGC 4 cut(s) 116, 269, 405, 661
BstV2I GAAGAC 1 cut(s) 189
BstX2I RGATCY 1 cut(s) 95
BstYI RGATCY 1 cut(s) 95
BsuI GTATCC 1 cut(s) 822
BsuRI GGCC 5 cut(s) 592, 734, 770, 834, 904
BtsIMutI CAGTG 2 cut(s) 153, 615
BtuMI TCGCGA 1 cut(s) 693
Cac8I GCNNGC 1 cut(s) 906
CfoI GCGC 1 cut(s) 124
Cfr10I RCCGGY 2 cut(s) 475, 730
Cfr13I GGNCC 5 cut(s) 590, 768, 833, 857, 903
CseI GACGC 2 cut(s) 35, 110
CsiI ACCWGGT 1 cut(s) 697
Csp6I GTAC 4 cut(s) 18, 158, 230, 335
CviAII CATG 1 cut(s) 932
CviQI GTAC 4 cut(s) 18, 158, 230, 335
DdeI CTNAG 3 cut(s) 54, 398, 603
DpnI GATC 5 cut(s) 51, 97, 237, 243, 863
DpnII GATC 5 cut(s) 49, 95, 235, 241, 861
EaeI YGGCCR 1 cut(s) 732
Eam1104I CTCTTC 4 cut(s) 135, 267, 438, 508
EarI CTCTTC 4 cut(s) 135, 267, 438, 508
EciI GGCGGA 3 cut(s) 378, 781, 890
Eco130I CCWWGG 1 cut(s) 939
Eco24I GRGCYC 2 cut(s) 788, 885
Eco31I GGTCTC 3 cut(s) 376, 522, 667
Eco47I GGWCC 1 cut(s) 857
Eco57I CTGAAG 1 cut(s) 105
Eco88I CYCGRG 2 cut(s) 640, 677
Eco91I GGTNACC 1 cut(s) 726
EcoO65I GGTNACC 1 cut(s) 726
EcoRII CCWGG 4 cut(s) 659, 697, 794, 814
EcoT14I CCWWGG 1 cut(s) 939
EcoT38I GRGCYC 2 cut(s) 788, 885
ErhI CCWWGG 1 cut(s) 939
Esp3I CGTCTC 2 cut(s) 426, 558
FaeI CATG 1 cut(s) 935
FaiI YATR 4 cut(s) 410, 588, 933, 961
FalI AAGNNNNNCTT 2 cut(s) 144, 176
FaqI GGGAC 1 cut(s) 512
FatI CATG 1 cut(s) 931
FauI CCCGC 3 cut(s) 70, 884, 913
FriOI GRGCYC 2 cut(s) 788, 885
FspBI CTAG 1 cut(s) 233
GlaI GCGC 1 cut(s) 123
HaeII RGCGCY 1 cut(s) 125
HaeIII GGCC 5 cut(s) 592, 734, 770, 834, 904
HapII CCGG 9 cut(s) 30, 110, 314, 476, 496, 575, 731, 804, 865
HgaI GACGC 2 cut(s) 35, 110
HhaI GCGC 1 cut(s) 124
Hin1I GRCGYC 1 cut(s) 27
Hin1II CATG 1 cut(s) 935
Hin6I GCGC 1 cut(s) 122
HinP1I GCGC 1 cut(s) 122
HincII GTYRAC 1 cut(s) 466
HindII GTYRAC 1 cut(s) 466
HinfI GANTC 7 cut(s) 500, 534, 566, 605, 643, 680, 914
HpaII CCGG 9 cut(s) 30, 110, 314, 476, 496, 575, 731, 804, 865
HphI GGTGA 4 cut(s) 180, 490, 713, 720
Hpy166II GTNNAC 1 cut(s) 466
Hpy188III TCNNGA 7 cut(s) 93, 239, 672, 692, 804, 865, 920
Hpy8I GTNNAC 1 cut(s) 466
Hpy99I CGWCG 3 cut(s) 440, 443, 693
HpyAV CCTTC 2 cut(s) 80, 195
HpyCH4III ACNGT 3 cut(s) 148, 424, 689
HpyCH4V TGCA 2 cut(s) 345, 393
HpyF10VI GCNNNNNNNGC 4 cut(s) 199, 208, 399, 414
HpyF3I CTNAG 3 cut(s) 54, 398, 603
Hsp92I GRCGYC 1 cut(s) 27
Hsp92II CATG 1 cut(s) 935
HspAI GCGC 1 cut(s) 122
Kpn2I TCCGGA 2 cut(s) 803, 864
Kzo9I GATC 5 cut(s) 49, 95, 235, 241, 861
LguI GCTCTTC 1 cut(s) 267
LmnI GCTCC 3 cut(s) 624, 783, 880
Lsp1109I GCAGC 4 cut(s) 116, 269, 405, 661
MabI ACCWGGT 1 cut(s) 697
MaeI CTAG 1 cut(s) 233
MaeIII GTNAC 4 cut(s) 424, 726, 742, 896
MalI GATC 5 cut(s) 51, 97, 237, 243, 863
MboI GATC 5 cut(s) 49, 95, 235, 241, 861
MboII GAAGA 6 cut(s) 152, 189, 281, 284, 455, 495
MfeI CAATTG 2 cut(s) 828, 837
MflI RGATCY 1 cut(s) 95
MhlI GDGCHC 3 cut(s) 419, 788, 885
MluCI AATT 4 cut(s) 305, 482, 828, 837
MlyI GAGTC 6 cut(s) 494, 543, 614, 652, 689, 923
MmeI TCCRAC 3 cut(s) 363, 560, 761
MroI TCCGGA 2 cut(s) 803, 864
MroXI GAANNNNTTC 1 cut(s) 447
MseI TTAA 1 cut(s) 762
MspA1I CMGCKG 1 cut(s) 891
MspI CCGG 9 cut(s) 30, 110, 314, 476, 496, 575, 731, 804, 865
MspR9I CCNGG 8 cut(s) 110, 314, 497, 576, 661, 699, 796, 816
MunI CAATTG 2 cut(s) 828, 837
MvaI CCWGG 4 cut(s) 661, 699, 796, 816
MvnI CGCG 1 cut(s) 693
MwoI GCNNNNNNNGC 4 cut(s) 199, 208, 399, 414
NciI CCSGG 4 cut(s) 110, 314, 497, 576
NdeII GATC 5 cut(s) 49, 95, 235, 241, 861
NlaIII CATG 1 cut(s) 935
NlaIV GGNNCC 3 cut(s) 418, 785, 882
NmuCI GTSAC 1 cut(s) 726
NruI TCGCGA 1 cut(s) 693
NspI RCATGY 1 cut(s) 935
PciI ACATGT 1 cut(s) 931
PciSI GCTCTTC 1 cut(s) 267
PcsI WCGNNNNNNNCGW 2 cut(s) 170, 918
PdmI GAANNNNTTC 1 cut(s) 447
PfeI GAWTC 1 cut(s) 566
PflFI GACNNNGTC 1 cut(s) 533
PflMI CCANNNNNTGG 1 cut(s) 73
PleI GAGTC 6 cut(s) 494, 542, 613, 651, 688, 922
PpsI GAGTC 6 cut(s) 494, 542, 613, 651, 688, 922
PscI ACATGT 1 cut(s) 931
PshAI GACNNNNGTC 1 cut(s) 679
Psp6I CCWGG 4 cut(s) 659, 697, 794, 814
PspEI GGTNACC 1 cut(s) 726
PspGI CCWGG 4 cut(s) 659, 697, 794, 814
PspN4I GGNNCC 3 cut(s) 418, 785, 882
PspPI GGNCC 5 cut(s) 590, 768, 833, 857, 903
PsuI RGATCY 1 cut(s) 95
PsyI GACNNNGTC 1 cut(s) 533
RruI TCGCGA 1 cut(s) 693
RsaI GTAC 4 cut(s) 19, 159, 231, 336
RsaNI GTAC 4 cut(s) 18, 158, 230, 335
SapI GCTCTTC 1 cut(s) 267
SaqAI TTAA 1 cut(s) 762
Sau3AI GATC 5 cut(s) 49, 95, 235, 241, 861
Sau96I GGNCC 5 cut(s) 590, 768, 833, 857, 903
ScaI AGTACT 2 cut(s) 159, 231
SchI GAGTC 6 cut(s) 494, 543, 614, 652, 689, 923
ScrFI CCNGG 8 cut(s) 110, 314, 497, 576, 661, 699, 796, 816
SduI GDGCHC 3 cut(s) 419, 788, 885
SexAI ACCWGGT 1 cut(s) 697
SgrAI CRCCGGYG 1 cut(s) 475
SinI GGWCC 1 cut(s) 857
Sse9I AATT 4 cut(s) 305, 482, 828, 837
SspMI CTAG 1 cut(s) 233
StyD4I CCNGG 8 cut(s) 108, 312, 495, 574, 659, 697, 794, 814
StyI CCWWGG 1 cut(s) 939
TaaI ACNGT 3 cut(s) 148, 424, 689
TaqI TCGA 7 cut(s) 48, 238, 309, 671, 820, 848, 921
TaqII GACCGA 1 cut(s) 546
TasI AATT 4 cut(s) 305, 482, 828, 837
TatI WGTACW 2 cut(s) 157, 229
TauI GCSGC 6 cut(s) 127, 130, 205, 208, 211, 363
TfiI GAWTC 1 cut(s) 566
Tru1I TTAA 1 cut(s) 762
Tru9I TTAA 1 cut(s) 762
TscAI CASTG 2 cut(s) 153, 615
TseFI GTSAC 1 cut(s) 726
TseI GCWGC 4 cut(s) 104, 257, 393, 649
Tsp45I GTSAC 1 cut(s) 726
TspDTI ATGAA 1 cut(s) 440
TspGWI ACGGA 1 cut(s) 540
TspRI CASTG 2 cut(s) 153, 615
Tth111I GACNNNGTC 1 cut(s) 533
Van91I CCANNNNNTGG 1 cut(s) 73
VpaK11BI GGWCC 1 cut(s) 857
XapI RAATTY 1 cut(s) 305
XceI RCATGY 1 cut(s) 935
XmnI GAANNNNTTC 1 cut(s) 447
XspI CTAG 1 cut(s) 233
ZrmI AGTACT 2 cut(s) 159, 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.