pycom10g25340

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0014354
Physical Location & Seq
Reverse (-)
26999771 .. 27000739
969 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g25340.1

Sequence Viewer

Length: 969 bp
ATGGGTTGCTGTTTAAGCACCGCCGCCGCTGGAAAGTCCTCCGCTTTAGGTCCTCAGAAGCACGGGCATTCATTGGTGGGCAATGAAGAGTCAAGATCTGGCGCCGCCCGCGAGAGTAGAGCACCGCCGCCAGTAGACGAAGAGACAGTGAAAGAAGTGCTGTCGGAGACACCGAAACCCAAAACCAAACCTCCACCGCAGTCTTCGCCGCCGCCGCTGTTCGAACACGAGCCAGTACTAGATCGAGATCAGGAAAAAAGAGCAGCTTCCGACGAAGAAGAGCTACCAGTGTTCGTTTCGTTGAAAACTAAACTCGACCCAGAAAAGATTGAGCAGAAAATACCCATTAGCAACAACAATGGCGGCGGCGGAGGAGAGATCTCGGAGGTGTCGGAGATTTGCAGCCTGAGCGAGAGCATGTCGGCCACCACCTTCACCAGAGACGACGACGAAGAGGTTCATCAGAAGTTAGTCAACAGGTCGCCGATGAAATTACGACAAAACCGGGACTCTTCAATGGGTCAAAGGAGAGACCGGGTTGTGGGTAAGTCACCGACTCGGAGAACGGAATCCTCTCCGGGTCGGAGATATGGGCCAAATGGTGCTGGGTCAGCGAGGTTGGTTCAGAGCAGAGAACCGGGCCCGGGTCAGCAGCCAATGTCCAGGCGTGGGCCGAGAACCGAGTCCAACCGGTGTGATCCGGGCGAGAGTTCCGGTCGGAGGTCTAGGTCACCCGCCATGAGGATTACGGACGGCGGTGGGGTTAATAGGACCAATGCAGGTAGGAGCCCCTCGCCCAGAAGATCCGGAAAGTATCCGACTCGAACTGCAATTGGTCCAATTGAGAGTTCGAGCTCTGGTCCGACCCGGAGAGTGGCTGAGGAGCCCAACAAGGAGGGTACGTGGCCCACCATCAATGAGTCTCTCGACAATCCGCATGTTTCCTTGGAGTGCTTTATTTTCCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

323

Amino Acids

34.88

Weight (kDa)

8.98

Isoelectric Point (pI)

84.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11125 AT1G61170
fragaria_vesca FvH4_3g04520
malus_domestica MD10G1300500.v1.1
prunus_persica Prupe.4G040000_v2.0.a1
pyrus_communis pycom05g29690 pycom10g25340
rosa_chinensis RchiOBHm_Chr5g0007441
rosa_laevigata RLG00000031457
rosa_multiflora Rmu_co8198018.1_g000001
rosa_roxburghii Rroxscaffold_1G00069030
rosa_rugosa Rorug04G0426900
rosa_samantha Rh5AG055300 Rh5BG052900 Rh5CG062700 Rh5DG051900
rosa_wichuraiana Rw5G005480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 770
AccB1I GGYRCC 1 cut(s) 101
AccI GTMKAC 1 cut(s) 135
AccII CGCG 1 cut(s) 111
AccIII TCCGGA 1 cut(s) 806
AclWI GGATC 2 cut(s) 692, 798
AcoI YGGCCR 1 cut(s) 423
AcyI GRCGYC 1 cut(s) 102
AfaI GTAC 2 cut(s) 237, 901
AfiI CCNNNNNNNGG 6 cut(s) 541, 644, 669, 720, 741, 874
AgeI ACCGGT 1 cut(s) 690
AgsI TTSAA 2 cut(s) 304, 516
AjnI CCWGG 1 cut(s) 662
AjuI GAANNNNNNNTTGG 2 cut(s) 832, 864
AluBI AGCT 3 cut(s) 266, 283, 855
AluI AGCT 3 cut(s) 266, 283, 855
Alw21I GWGCWC 2 cut(s) 124, 857
Alw26I GTCTC 5 cut(s) 137, 161, 435, 525, 927
AlwI GGATC 2 cut(s) 692, 798
Ama87I CYCGRG 1 cut(s) 643
Aor13HI TCCGGA 1 cut(s) 806
AoxI GGCC 5 cut(s) 423, 593, 640, 671, 905
ApaI GGGCCC 1 cut(s) 644
ApeKI GCWGC 3 cut(s) 263, 402, 652
AsiGI ACCGGT 1 cut(s) 690
Asp700I GAANNNNTTC 1 cut(s) 456
AspLEI GCGC 1 cut(s) 104
AspS9I GGNCC 9 cut(s) 50, 593, 640, 641, 671, 771, 836, 860, 906
AsuC2I CCSGG 8 cut(s) 506, 536, 579, 639, 644, 645, 702, 868
AsuHPI GGTGA 3 cut(s) 427, 543, 723
AsuII TTCGAA 1 cut(s) 222
AvaI CYCGRG 1 cut(s) 643
AvaII GGWCC 4 cut(s) 50, 771, 836, 860
BaeGI GKGCMC 1 cut(s) 644
BanI GGYRCC 1 cut(s) 101
BanII GRGCYC 4 cut(s) 644, 791, 857, 888
BarI GAAGNNNNNNTAC 2 cut(s) 267, 299
BauI CACGAG 1 cut(s) 227
BbsI GAAGAC 1 cut(s) 195
Bbv12I GWGCWC 2 cut(s) 124, 857
BbvCI CCTCAGC 1 cut(s) 879
BbvI GCAGC 3 cut(s) 275, 414, 664
BccI CCATC 1 cut(s) 920
BceAI ACGGC 1 cut(s) 769
BciT130I CCWGG 1 cut(s) 664
BciVI GTATCC 1 cut(s) 825
BcnI CCSGG 8 cut(s) 506, 536, 579, 639, 644, 645, 702, 868
BcoDI GTCTC 5 cut(s) 137, 161, 435, 525, 927
BfaI CTAG 2 cut(s) 239, 726
BfoI RGCGCY 1 cut(s) 105
BfuAI ACCTGC 1 cut(s) 770
BfuI GTATCC 1 cut(s) 825
BglII AGATCT 2 cut(s) 95, 378
BmcAI AGTACT 1 cut(s) 237
Bme1390I CCNGG 9 cut(s) 506, 536, 579, 639, 644, 645, 664, 702, 868
Bme18I GGWCC 4 cut(s) 50, 771, 836, 860
BmeT110I CYCGRG 1 cut(s) 643
BmgT120I GGNCC 9 cut(s) 50, 593, 640, 641, 671, 771, 836, 860, 906
BmiI GGNNCC 4 cut(s) 103, 642, 788, 885
BmrFI CCNGG 9 cut(s) 506, 536, 579, 639, 644, 645, 664, 702, 868
BpiI GAAGAC 1 cut(s) 195
Bpu10I CCTNAGC 2 cut(s) 407, 879
Bpu14I TTCGAA 1 cut(s) 222
BpuMI CCSGG 8 cut(s) 506, 536, 579, 639, 644, 645, 702, 868
BsaAI YACGTR 1 cut(s) 903
BsaBI GATNNNNATC 1 cut(s) 246
BsaHI GRCGYC 1 cut(s) 102
BsaI GGTCTC 1 cut(s) 525
BsaJI CCNNGG 2 cut(s) 643, 945
BsaWI WCCGGW 3 cut(s) 690, 713, 806
BsaXI ACNNNNNCTCC 4 cut(s) 175, 205, 887, 917
Bsc4I CCNNNNNNNGG 6 cut(s) 541, 644, 669, 720, 741, 874
Bse118I RCCGGY 1 cut(s) 690
Bse1I ACTGG 3 cut(s) 131, 233, 287
Bse3DI GCAATG 1 cut(s) 88
Bse8I GATNNNNATC 1 cut(s) 246
BseAI TCCGGA 1 cut(s) 806
BseBI CCWGG 1 cut(s) 664
BseDI CCNNGG 2 cut(s) 643, 945
BseJI GATNNNNATC 1 cut(s) 246
BseLI CCNNNNNNNGG 6 cut(s) 541, 644, 669, 720, 741, 874
BseMI GCAATG 1 cut(s) 88
BseMII CTCAG 3 cut(s) 68, 398, 870
BseNI ACTGG 3 cut(s) 131, 233, 287
BseRI GAGGAG 2 cut(s) 387, 896
BseSI GKGCMC 1 cut(s) 644
BseXI GCAGC 3 cut(s) 275, 414, 664
BseYI CCCAGC 1 cut(s) 605
Bsh1236I CGCG 1 cut(s) 111
Bsh1285I CGRYCG 1 cut(s) 718
BshFI GGCC 5 cut(s) 425, 595, 642, 673, 907
BshNI GGYRCC 1 cut(s) 101
BshTI ACCGGT 1 cut(s) 690
BsiEI CGRYCG 1 cut(s) 718
BsiHKAI GWGCWC 2 cut(s) 124, 857
BsiHKCI CYCGRG 1 cut(s) 643
BslFI GGGAC 1 cut(s) 521
BslI CCNNNNNNNGG 6 cut(s) 541, 644, 669, 720, 741, 874
BsmAI GTCTC 5 cut(s) 137, 161, 435, 525, 927
BsmBI CGTCTC 1 cut(s) 435
BsmFI GGGAC 1 cut(s) 521
BsmI GAATGC 1 cut(s) 67
BsnI GGCC 5 cut(s) 425, 595, 642, 673, 907
Bso31I GGTCTC 1 cut(s) 525
BsoBI CYCGRG 1 cut(s) 643
Bsp119I TTCGAA 1 cut(s) 222
Bsp120I GGGCCC 1 cut(s) 640
Bsp1286I GDGCHC 5 cut(s) 124, 644, 791, 857, 888
Bsp13I TCCGGA 1 cut(s) 806
Bsp143I GATC 6 cut(s) 95, 241, 247, 378, 697, 803
BspANI GGCC 5 cut(s) 425, 595, 642, 673, 907
BspCNI CTCAG 3 cut(s) 67, 399, 871
BspEI TCCGGA 1 cut(s) 806
BspFNI CGCG 1 cut(s) 111
BspLI GGNNCC 4 cut(s) 103, 642, 788, 885
BspMI ACCTGC 1 cut(s) 770
BspPI GGATC 2 cut(s) 692, 798
BspQI GCTCTTC 1 cut(s) 273
BspT104I TTCGAA 1 cut(s) 222
BspT107I GGYRCC 1 cut(s) 101
BspTNI GGTCTC 1 cut(s) 525
BsrDI GCAATG 1 cut(s) 88
BsrFI RCCGGY 1 cut(s) 690
BsrI ACTGG 3 cut(s) 131, 233, 287
BssAI RCCGGY 1 cut(s) 690
BssECI CCNNGG 2 cut(s) 643, 945
BssMI GATC 6 cut(s) 95, 241, 247, 378, 697, 803
BssNI GRCGYC 1 cut(s) 102
BssSI CACGAG 1 cut(s) 227
BssT1I CCWWGG 1 cut(s) 945
Bst2BI CACGAG 1 cut(s) 227
Bst2UI CCWGG 1 cut(s) 664
Bst4CI ACNGT 1 cut(s) 148
Bst6I CTCTTC 5 cut(s) 81, 135, 273, 447, 517
BstACI GRCGYC 1 cut(s) 102
BstBAI YACGTR 1 cut(s) 903
BstBI TTCGAA 1 cut(s) 222
BstC8I GCNNGC 1 cut(s) 109
BstDEI CTNAG 3 cut(s) 54, 407, 879
BstEII GGTNACC 1 cut(s) 729
BstFNI CGCG 1 cut(s) 111
BstH2I RGCGCY 1 cut(s) 105
BstHHI GCGC 1 cut(s) 104
BstKTI GATC 6 cut(s) 98, 244, 250, 381, 700, 806
BstMAI GTCTC 5 cut(s) 137, 161, 435, 525, 927
BstMBI GATC 6 cut(s) 95, 241, 247, 378, 697, 803
BstMCI CGRYCG 1 cut(s) 718
BstMWI GCNNNNNNNGC 6 cut(s) 15, 108, 205, 214, 408, 611
BstNI CCWGG 1 cut(s) 664
BstNSI RCATGY 2 cut(s) 421, 941
BstPI GGTNACC 1 cut(s) 729
BstSCI CCNGG 9 cut(s) 504, 534, 577, 637, 642, 643, 662, 700, 866
BstSLI GKGCMC 1 cut(s) 644
BstUI CGCG 1 cut(s) 111
BstV1I GCAGC 3 cut(s) 275, 414, 664
BstV2I GAAGAC 1 cut(s) 195
BstX2I RGATCY 3 cut(s) 95, 378, 803
BstYI RGATCY 3 cut(s) 95, 378, 803
BsuI GTATCC 1 cut(s) 825
BsuRI GGCC 5 cut(s) 425, 595, 642, 673, 907
BtsIMutI CAGTG 2 cut(s) 153, 294
BveI ACCTGC 1 cut(s) 770
Cac8I GCNNGC 1 cut(s) 109
CfoI GCGC 1 cut(s) 104
Cfr10I RCCGGY 1 cut(s) 690
Cfr13I GGNCC 9 cut(s) 50, 593, 640, 641, 671, 771, 836, 860, 906
Cfr9I CCCGGG 1 cut(s) 643
Csp6I GTAC 2 cut(s) 236, 900
CspAI ACCGGT 1 cut(s) 690
CviAII CATG 3 cut(s) 418, 739, 938
CviQI GTAC 2 cut(s) 236, 900
DdeI CTNAG 3 cut(s) 54, 407, 879
DinI GGCGCC 1 cut(s) 103
DpnI GATC 6 cut(s) 97, 243, 249, 380, 699, 805
DpnII GATC 6 cut(s) 95, 241, 247, 378, 697, 803
EaeI YGGCCR 1 cut(s) 423
Eam1104I CTCTTC 5 cut(s) 81, 135, 273, 447, 517
EarI CTCTTC 5 cut(s) 81, 135, 273, 447, 517
EciI GGCGGA 1 cut(s) 384
Ecl136II GAGCTC 1 cut(s) 855
Eco130I CCWWGG 1 cut(s) 945
Eco24I GRGCYC 4 cut(s) 644, 791, 857, 888
Eco31I GGTCTC 1 cut(s) 525
Eco47I GGWCC 4 cut(s) 50, 771, 836, 860
Eco53kI GAGCTC 1 cut(s) 855
Eco88I CYCGRG 1 cut(s) 643
Eco91I GGTNACC 1 cut(s) 729
EcoICRI GAGCTC 1 cut(s) 855
EcoO109I RGGNCCY 1 cut(s) 50
EcoO65I GGTNACC 1 cut(s) 729
EcoRII CCWGG 1 cut(s) 662
EcoT14I CCWWGG 1 cut(s) 945
EcoT38I GRGCYC 4 cut(s) 644, 791, 857, 888
EgeI GGCGCC 1 cut(s) 103
EheI GGCGCC 1 cut(s) 103
ErhI CCWWGG 1 cut(s) 945
Esp3I CGTCTC 1 cut(s) 435
FaeI CATG 3 cut(s) 421, 742, 941
FaiI YATR 5 cut(s) 419, 591, 740, 939, 967
FalI AAGNNNNNCTT 2 cut(s) 250, 282
FaqI GGGAC 1 cut(s) 521
FatI CATG 3 cut(s) 417, 738, 937
FauI CCCGC 2 cut(s) 116, 742
FblI GTMKAC 1 cut(s) 135
FriOI GRGCYC 4 cut(s) 644, 791, 857, 888
FspBI CTAG 2 cut(s) 239, 726
GlaI GCGC 1 cut(s) 103
GsaI CCCAGC 1 cut(s) 609
HaeII RGCGCY 1 cut(s) 105
HaeIII GGCC 5 cut(s) 425, 595, 642, 673, 907
HhaI GCGC 1 cut(s) 104
Hin1I GRCGYC 1 cut(s) 102
Hin1II CATG 3 cut(s) 421, 742, 941
Hin6I GCGC 1 cut(s) 102
HinP1I GCGC 1 cut(s) 102
HincII GTYRAC 1 cut(s) 475
HindII GTYRAC 1 cut(s) 475
HinfI GANTC 7 cut(s) 89, 509, 556, 569, 683, 820, 920
HphI GGTGA 3 cut(s) 427, 543, 723
Hpy166II GTNNAC 2 cut(s) 136, 475
Hpy188III TCNNGA 5 cut(s) 93, 245, 251, 807, 926
Hpy8I GTNNAC 2 cut(s) 136, 475
Hpy99I CGWCG 3 cut(s) 275, 449, 452
HpyAV CCTTC 1 cut(s) 442
HpyCH4III ACNGT 1 cut(s) 148
HpyCH4IV ACGT 1 cut(s) 902
HpyCH4V TGCA 3 cut(s) 402, 779, 830
HpyF10VI GCNNNNNNNGC 6 cut(s) 15, 108, 205, 214, 408, 611
HpyF3I CTNAG 3 cut(s) 54, 407, 879
HpySE526I ACGT 1 cut(s) 902
Hsp92I GRCGYC 1 cut(s) 102
Hsp92II CATG 3 cut(s) 421, 742, 941
HspAI GCGC 1 cut(s) 102
KasI GGCGCC 1 cut(s) 101
Kpn2I TCCGGA 1 cut(s) 806
Kzo9I GATC 6 cut(s) 95, 241, 247, 378, 697, 803
LguI GCTCTTC 1 cut(s) 273
LmnI GCTCC 2 cut(s) 786, 883
Lsp1109I GCAGC 3 cut(s) 275, 414, 664
MaeI CTAG 2 cut(s) 239, 726
MaeII ACGT 1 cut(s) 902
MaeIII GTNAC 2 cut(s) 549, 729
MalI GATC 6 cut(s) 97, 243, 249, 380, 699, 805
MboI GATC 6 cut(s) 95, 241, 247, 378, 697, 803
MboII GAAGA 8 cut(s) 98, 152, 195, 287, 290, 464, 504, 813
MfeI CAATTG 2 cut(s) 831, 840
MflI RGATCY 3 cut(s) 95, 378, 803
MhlI GDGCHC 5 cut(s) 124, 644, 791, 857, 888
MluCI AATT 3 cut(s) 491, 831, 840
Mly113I GGCGCC 1 cut(s) 102
MlyI GAGTC 6 cut(s) 98, 503, 550, 692, 814, 929
MmeI TCCRAC 8 cut(s) 144, 294, 372, 563, 698, 711, 842, 887
MroI TCCGGA 1 cut(s) 806
MroXI GAANNNNTTC 1 cut(s) 456
MseI TTAA 2 cut(s) 14, 765
MspA1I CMGCKG 2 cut(s) 29, 217
MspR9I CCNGG 9 cut(s) 506, 536, 579, 639, 644, 645, 664, 702, 868
MunI CAATTG 2 cut(s) 831, 840
Mva1269I GAATGC 1 cut(s) 67
MvaI CCWGG 1 cut(s) 664
MvnI CGCG 1 cut(s) 111
MwoI GCNNNNNNNGC 6 cut(s) 15, 108, 205, 214, 408, 611
NarI GGCGCC 1 cut(s) 102
NciI CCSGG 8 cut(s) 506, 536, 579, 639, 644, 645, 702, 868
NdeII GATC 6 cut(s) 95, 241, 247, 378, 697, 803
NlaIII CATG 3 cut(s) 421, 742, 941
NlaIV GGNNCC 4 cut(s) 103, 642, 788, 885
NmeAIII GCCGAG 1 cut(s) 699
NmuCI GTSAC 2 cut(s) 549, 729
NspI RCATGY 2 cut(s) 421, 941
NspV TTCGAA 1 cut(s) 222
PciSI GCTCTTC 1 cut(s) 273
PcsI WCGNNNNNNNCGW 1 cut(s) 170
PctI GAATGC 1 cut(s) 67
PdmI GAANNNNTTC 1 cut(s) 456
PfeI GAWTC 1 cut(s) 569
PinAI ACCGGT 1 cut(s) 690
PleI GAGTC 6 cut(s) 97, 503, 550, 691, 814, 928
PluTI GGCGCC 1 cut(s) 105
PpsI GAGTC 6 cut(s) 97, 503, 550, 691, 814, 928
Ppu21I YACGTR 1 cut(s) 903
PpuMI RGGWCCY 1 cut(s) 50
Psp124BI GAGCTC 1 cut(s) 857
Psp5II RGGWCCY 1 cut(s) 50
Psp6I CCWGG 1 cut(s) 662
PspEI GGTNACC 1 cut(s) 729
PspFI CCCAGC 1 cut(s) 605
PspGI CCWGG 1 cut(s) 662
PspN4I GGNNCC 4 cut(s) 103, 642, 788, 885
PspOMI GGGCCC 1 cut(s) 640
PspPI GGNCC 9 cut(s) 50, 593, 640, 641, 671, 771, 836, 860, 906
PspPPI RGGWCCY 1 cut(s) 50
PsuI RGATCY 3 cut(s) 95, 378, 803
RsaI GTAC 2 cut(s) 237, 901
RsaNI GTAC 2 cut(s) 236, 900
SacI GAGCTC 1 cut(s) 857
SapI GCTCTTC 1 cut(s) 273
SaqAI TTAA 2 cut(s) 14, 765
Sau3AI GATC 6 cut(s) 95, 241, 247, 378, 697, 803
Sau96I GGNCC 9 cut(s) 50, 593, 640, 641, 671, 771, 836, 860, 906
ScaI AGTACT 1 cut(s) 237
SchI GAGTC 6 cut(s) 98, 503, 550, 692, 814, 929
ScrFI CCNGG 9 cut(s) 506, 536, 579, 639, 644, 645, 664, 702, 868
SduI GDGCHC 5 cut(s) 124, 644, 791, 857, 888
SfoI GGCGCC 1 cut(s) 103
SfuI TTCGAA 1 cut(s) 222
SinI GGWCC 4 cut(s) 50, 771, 836, 860
SmaI CCCGGG 1 cut(s) 645
Sse9I AATT 3 cut(s) 491, 831, 840
SspDI GGCGCC 1 cut(s) 101
SspMI CTAG 2 cut(s) 239, 726
SstI GAGCTC 1 cut(s) 857
StyD4I CCNGG 9 cut(s) 504, 534, 577, 637, 642, 643, 662, 700, 866
StyI CCWWGG 1 cut(s) 945
TaaI ACNGT 1 cut(s) 148
TaiI ACGT 1 cut(s) 905
TaqI TCGA 6 cut(s) 222, 244, 315, 823, 851, 927
TasI AATT 3 cut(s) 491, 831, 840
TatI WGTACW 1 cut(s) 235
TauI GCSGC 9 cut(s) 26, 29, 107, 130, 211, 214, 217, 366, 369
TfiI GAWTC 1 cut(s) 569
Tru1I TTAA 2 cut(s) 14, 765
Tru9I TTAA 2 cut(s) 14, 765
TscAI CASTG 2 cut(s) 153, 294
TseFI GTSAC 2 cut(s) 549, 729
TseI GCWGC 3 cut(s) 263, 402, 652
Tsp45I GTSAC 2 cut(s) 549, 729
TspDTI ATGAA 4 cut(s) 60, 99, 449, 503
TspGWI ACGGA 2 cut(s) 581, 764
TspMI CCCGGG 1 cut(s) 643
TspRI CASTG 2 cut(s) 153, 294
VpaK11BI GGWCC 4 cut(s) 50, 771, 836, 860
XceI RCATGY 2 cut(s) 421, 941
XmaI CCCGGG 1 cut(s) 643
XmiI GTMKAC 1 cut(s) 135
XmnI GAANNNNTTC 1 cut(s) 456
XspI CTAG 2 cut(s) 239, 726
ZrmI AGTACT 1 cut(s) 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.