Rw5G005480

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0014354
Physical Location & Seq
Reverse (-)
5739936 .. 5741312
1377 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G005480.1

Sequence Viewer

Length: 939 bp
ATGGGGTGCTGTCTCAGCTCCTCCGCCACCGACAAGTCCTCAGCTCCCAATCAGCCCCCACATTTCCAGAGATCCGACGACAGCAGAGCTCCGCCGCCGGTGGTGGACGAAGAGACCGTCAAGGAAGTGCTTTCAGTTTCAGAGACACCGAGGCCGAAGCCAACCCAAGAACAACAAGAACCAGTGTTTCAAGAACAGAAGAGGACCCAAGAACCGGTGTTTGAAGTGAAACTGGATCGGGTTCAAGAACAGAAGAGGGTCGTCGACGTCCAAGAACTACTACCGGTCTTTGAGGCGGAGGAGAACTGGGAGAAGAGAATACCCATTTCCGCCGGCGGCGACGGAGAGATCTCGGAGCAGGCGTCGGAGATTTGCAGCCTGCAGAGCGAGAGCTTCTCGGCGACGACGGTAACCAGAGACGAAGACGATGAGGTTCAACAGAGGGTAGTCAACAACAGCAGCAGCAACAGAGTGTCGCCGGTGAAATTACAGAAAAACCCTCGGGACGTCGGGTTTAGGCCGGGCCGGGTGGTGGGTAAGTCTCCGACGCGGAGGACGGACGCGTCGCCGGGTCGGAGGTACGGTCCGAATGGGAATGCAGTCGGGTCAGCGAGGGTGGTTCAACCGGGTCAGGGTGGTAGGCGGGTCGGGTCGAGGACGGAGTCGAACCGGCGTGACCCGGGTGAGAGCTCGGGTCGGAGGTCCAGATCGCCGGCGACGAGGATTGTGGAGAACGGTGGAGTTAATAGAGCAAATGTGGGTCGGAGCCCGTCCGCTAATAGGAGGTATCCGGGTCGGAGTCCGGTTGGTCCGGTTGAGAGTTCGAATAGTTCGACCCGGAGAATATTTGAGGAGCCCAAAATGGAGGAGGGTAAATGGCCCGCAAATGAGTCTCTTGATAACCCACATGTTTCTTTGGAATGCTTCATTTTCTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

312

Amino Acids

34.43

Weight (kDa)

7.66

Isoelectric Point (pI)

80.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11125 AT1G61170
fragaria_vesca FvH4_3g04520
malus_domestica MD10G1300500.v1.1
prunus_persica Prupe.4G040000_v2.0.a1
pyrus_communis pycom05g29690 pycom10g25340
rosa_chinensis RchiOBHm_Chr5g0007441
rosa_laevigata RLG00000031457
rosa_multiflora Rmu_co8198018.1_g000001
rosa_roxburghii Rroxscaffold_1G00069030
rosa_rugosa Rorug04G0426900
rosa_samantha Rh5AG055300 Rh5BG052900 Rh5CG062700 Rh5DG051900
rosa_wichuraiana Rw5G005480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 562
AatII GACGTC 2 cut(s) 270, 510
AccI GTMKAC 1 cut(s) 264
AccII CGCG 2 cut(s) 550, 563
AclWI GGATC 2 cut(s) 66, 243
AcyI GRCGYC 3 cut(s) 267, 362, 507
AfaI GTAC 1 cut(s) 581
AfiI CCNNNNNNNGG 4 cut(s) 214, 532, 632, 780
AflIII ACRYGT 2 cut(s) 561, 907
AgeI ACCGGT 2 cut(s) 214, 283
AgsI TTSAA 5 cut(s) 191, 224, 245, 437, 623
AluBI AGCT 5 cut(s) 18, 44, 89, 393, 690
AluI AGCT 5 cut(s) 18, 44, 89, 393, 690
Alw21I GWGCWC 2 cut(s) 91, 692
Alw26I GTCTC 6 cut(s) 17, 107, 137, 411, 546, 897
AlwI GGATC 2 cut(s) 66, 243
Ama87I CYCGRG 3 cut(s) 501, 679, 691
AoxI GGCC 4 cut(s) 152, 518, 523, 878
ApeKI GCWGC 3 cut(s) 375, 459, 462
ArsI GACNNNNNNTTYG 2 cut(s) 102, 134
AsiGI ACCGGT 2 cut(s) 214, 283
AspS9I GGNCC 6 cut(s) 204, 523, 584, 702, 809, 879
AsuC2I CCSGG 8 cut(s) 522, 527, 570, 627, 680, 681, 792, 838
AsuHPI GGTGA 2 cut(s) 493, 695
AsuII TTCGAA 1 cut(s) 824
AvaI CYCGRG 3 cut(s) 501, 679, 691
AvaII GGWCC 4 cut(s) 204, 584, 702, 809
BanII GRGCYC 4 cut(s) 91, 692, 770, 858
BbsI GAAGAC 1 cut(s) 429
Bbv12I GWGCWC 2 cut(s) 91, 692
BbvCI CCTCAGC 1 cut(s) 40
BbvI GCAGC 3 cut(s) 387, 471, 474
BciVI GTATCC 1 cut(s) 798
BcnI CCSGG 8 cut(s) 522, 527, 570, 627, 680, 681, 792, 838
BcoDI GTCTC 6 cut(s) 17, 107, 137, 411, 546, 897
BfmI CTRYAG 1 cut(s) 380
BfuI GTATCC 1 cut(s) 798
BglII AGATCT 1 cut(s) 348
BisI GCNGC 5 cut(s) 95, 337, 376, 460, 463
BlsI GCNGC 5 cut(s) 96, 338, 377, 461, 464
Bme1390I CCNGG 8 cut(s) 522, 527, 570, 627, 680, 681, 792, 838
Bme18I GGWCC 4 cut(s) 204, 584, 702, 809
BmeT110I CYCGRG 3 cut(s) 501, 679, 691
BmgT120I GGNCC 6 cut(s) 204, 523, 584, 702, 809, 879
BmiI GGNNCC 3 cut(s) 206, 767, 855
BmrFI CCNGG 8 cut(s) 522, 527, 570, 627, 680, 681, 792, 838
BmrI ACTGGG 1 cut(s) 316
BmuI ACTGGG 1 cut(s) 316
BpiI GAAGAC 1 cut(s) 429
BplI GAGNNNNNCTC 2 cut(s) 380, 412
Bpu10I CCTNAGC 1 cut(s) 40
Bpu14I TTCGAA 1 cut(s) 824
BpuMI CCSGG 8 cut(s) 522, 527, 570, 627, 680, 681, 792, 838
BsaHI GRCGYC 3 cut(s) 267, 362, 507
BsaI GGTCTC 1 cut(s) 107
BsaJI CCNNGG 3 cut(s) 149, 500, 679
BsaWI WCCGGW 4 cut(s) 214, 283, 802, 811
BsaXI ACNNNNNCTCC 4 cut(s) 347, 377, 568, 598
Bsc4I CCNNNNNNNGG 4 cut(s) 214, 532, 632, 780
Bse118I RCCGGY 7 cut(s) 97, 214, 283, 332, 478, 669, 712
Bse1I ACTGG 3 cut(s) 182, 237, 311
BseDI CCNNGG 3 cut(s) 149, 500, 679
BseLI CCNNNNNNNGG 4 cut(s) 214, 532, 632, 780
BseMII CTCAG 2 cut(s) 28, 54
BseNI ACTGG 3 cut(s) 182, 237, 311
BseRI GAGGAG 4 cut(s) 10, 314, 866, 881
BseXI GCAGC 3 cut(s) 387, 471, 474
Bsh1236I CGCG 2 cut(s) 550, 563
BshFI GGCC 4 cut(s) 154, 520, 525, 880
BshTI ACCGGT 2 cut(s) 214, 283
BsiHKAI GWGCWC 2 cut(s) 91, 692
BsiHKCI CYCGRG 3 cut(s) 501, 679, 691
BslFI GGGAC 1 cut(s) 518
BslI CCNNNNNNNGG 4 cut(s) 214, 532, 632, 780
BsmAI GTCTC 6 cut(s) 17, 107, 137, 411, 546, 897
BsmBI CGTCTC 1 cut(s) 411
BsmFI GGGAC 1 cut(s) 518
BsmI GAATGC 2 cut(s) 601, 926
BsnI GGCC 4 cut(s) 154, 520, 525, 880
Bso31I GGTCTC 1 cut(s) 107
BsoBI CYCGRG 3 cut(s) 501, 679, 691
Bsp119I TTCGAA 1 cut(s) 824
Bsp1286I GDGCHC 4 cut(s) 91, 692, 770, 858
Bsp143I GATC 4 cut(s) 71, 235, 348, 707
BspANI GGCC 4 cut(s) 154, 520, 525, 880
BspCNI CTCAG 2 cut(s) 27, 53
BspFNI CGCG 2 cut(s) 550, 563
BspLI GGNNCC 3 cut(s) 206, 767, 855
BspMAI CTGCAG 1 cut(s) 384
BspPI GGATC 2 cut(s) 66, 243
BspT104I TTCGAA 1 cut(s) 824
BspTNI GGTCTC 1 cut(s) 107
BsrFI RCCGGY 7 cut(s) 97, 214, 283, 332, 478, 669, 712
BsrI ACTGG 3 cut(s) 182, 237, 311
BssAI RCCGGY 7 cut(s) 97, 214, 283, 332, 478, 669, 712
BssECI CCNNGG 3 cut(s) 149, 500, 679
BssMI GATC 4 cut(s) 71, 235, 348, 707
BssNI GRCGYC 3 cut(s) 267, 362, 507
Bst4CI ACNGT 4 cut(s) 118, 409, 584, 737
Bst6I CTCTTC 4 cut(s) 105, 194, 248, 308
BstACI GRCGYC 3 cut(s) 267, 362, 507
BstBI TTCGAA 1 cut(s) 824
BstC8I GCNNGC 5 cut(s) 334, 360, 380, 714, 882
BstDEI CTNAG 2 cut(s) 14, 40
BstEII GGTNACC 1 cut(s) 409
BstFNI CGCG 2 cut(s) 550, 563
BstKTI GATC 4 cut(s) 74, 238, 351, 710
BstMAI GTCTC 6 cut(s) 17, 107, 137, 411, 546, 897
BstMBI GATC 4 cut(s) 71, 235, 348, 707
BstMWI GCNNNNNNNGC 2 cut(s) 15, 384
BstNSI RCATGY 1 cut(s) 911
BstPI GGTNACC 1 cut(s) 409
BstSCI CCNGG 8 cut(s) 520, 525, 568, 625, 678, 679, 790, 836
BstSFI CTRYAG 1 cut(s) 380
BstUI CGCG 2 cut(s) 550, 563
BstV1I GCAGC 3 cut(s) 387, 471, 474
BstV2I GAAGAC 1 cut(s) 429
BstX2I RGATCY 2 cut(s) 71, 348
BstYI RGATCY 2 cut(s) 71, 348
BsuI GTATCC 1 cut(s) 798
BsuRI GGCC 4 cut(s) 154, 520, 525, 880
BtsIMutI CAGTG 1 cut(s) 189
Cac8I GCNNGC 5 cut(s) 334, 360, 380, 714, 882
Cfr10I RCCGGY 7 cut(s) 97, 214, 283, 332, 478, 669, 712
Cfr13I GGNCC 6 cut(s) 204, 523, 584, 702, 809, 879
Cfr9I CCCGGG 1 cut(s) 679
CpoI CGGWCCG 1 cut(s) 584
CseI GACGC 4 cut(s) 351, 552, 556, 569
Csp6I GTAC 1 cut(s) 580
CspAI ACCGGT 2 cut(s) 214, 283
CspI CGGWCCG 1 cut(s) 584
CviAII CATG 1 cut(s) 908
CviQI GTAC 1 cut(s) 580
DdeI CTNAG 2 cut(s) 14, 40
DpnI GATC 4 cut(s) 73, 237, 350, 709
DpnII GATC 4 cut(s) 71, 235, 348, 707
DrdI GACNNNNNNGTC 1 cut(s) 562
DseDI GACNNNNNNGTC 1 cut(s) 562
Eam1104I CTCTTC 4 cut(s) 105, 194, 248, 308
EarI CTCTTC 4 cut(s) 105, 194, 248, 308
EciI GGCGGA 4 cut(s) 13, 81, 311, 319
Ecl136II GAGCTC 2 cut(s) 89, 690
Eco24I GRGCYC 4 cut(s) 91, 692, 770, 858
Eco31I GGTCTC 1 cut(s) 107
Eco47I GGWCC 4 cut(s) 204, 584, 702, 809
Eco53kI GAGCTC 2 cut(s) 89, 690
Eco88I CYCGRG 3 cut(s) 501, 679, 691
Eco91I GGTNACC 1 cut(s) 409
EcoICRI GAGCTC 2 cut(s) 89, 690
EcoO109I RGGNCCY 1 cut(s) 204
EcoO65I GGTNACC 1 cut(s) 409
EcoT38I GRGCYC 4 cut(s) 91, 692, 770, 858
Esp3I CGTCTC 1 cut(s) 411
FaeI CATG 1 cut(s) 911
FaiI YATR 2 cut(s) 909, 937
FaqI GGGAC 1 cut(s) 518
FatI CATG 1 cut(s) 907
FauI CCCGC 2 cut(s) 636, 889
FblI GTMKAC 1 cut(s) 264
Fnu4HI GCNGC 5 cut(s) 95, 337, 376, 460, 463
FriOI GRGCYC 4 cut(s) 91, 692, 770, 858
Fsp4HI GCNGC 5 cut(s) 95, 337, 376, 460, 463
GluI GCNGC 5 cut(s) 95, 337, 376, 460, 463
HaeIII GGCC 4 cut(s) 154, 520, 525, 880
HgaI GACGC 4 cut(s) 351, 552, 556, 569
Hin1I GRCGYC 3 cut(s) 267, 362, 507
Hin1II CATG 1 cut(s) 911
HincII GTYRAC 2 cut(s) 265, 451
HindII GTYRAC 2 cut(s) 265, 451
HinfI GANTC 3 cut(s) 662, 799, 890
HphI GGTGA 2 cut(s) 493, 695
Hpy166II GTNNAC 3 cut(s) 106, 265, 451
Hpy188III TCNNGA 6 cut(s) 67, 191, 245, 503, 705, 896
Hpy8I GTNNAC 3 cut(s) 106, 265, 451
HpyCH4III ACNGT 4 cut(s) 118, 409, 584, 737
HpyCH4IV ACGT 2 cut(s) 267, 507
HpyCH4V TGCA 3 cut(s) 375, 382, 599
HpyF10VI GCNNNNNNNGC 2 cut(s) 15, 384
HpyF3I CTNAG 2 cut(s) 14, 40
HpySE526I ACGT 2 cut(s) 267, 507
Hsp92I GRCGYC 3 cut(s) 267, 362, 507
Hsp92II CATG 1 cut(s) 911
KroI GCCGGC 2 cut(s) 332, 712
KroNI GCCGGC 2 cut(s) 334, 714
Kzo9I GATC 4 cut(s) 71, 235, 348, 707
LmnI GCTCC 6 cut(s) 23, 49, 94, 355, 765, 853
Lsp1109I GCAGC 3 cut(s) 387, 471, 474
MaeII ACGT 2 cut(s) 267, 507
MaeIII GTNAC 2 cut(s) 409, 674
MalI GATC 4 cut(s) 73, 237, 350, 709
MboI GATC 4 cut(s) 71, 235, 348, 707
MboII GAAGA 5 cut(s) 122, 211, 265, 325, 434
MflI RGATCY 2 cut(s) 71, 348
MhlI GDGCHC 4 cut(s) 91, 692, 770, 858
MluCI AATT 1 cut(s) 485
MluI ACGCGT 1 cut(s) 561
MlyI GAGTC 3 cut(s) 671, 808, 899
MmeI TCCRAC 7 cut(s) 99, 345, 554, 569, 677, 743, 776
MreI CGCCGGCG 2 cut(s) 332, 712
MroNI GCCGGC 2 cut(s) 332, 712
MseI TTAA 1 cut(s) 744
MspR9I CCNGG 8 cut(s) 522, 527, 570, 627, 680, 681, 792, 838
Mva1269I GAATGC 2 cut(s) 601, 926
MvnI CGCG 2 cut(s) 550, 563
MwoI GCNNNNNNNGC 2 cut(s) 15, 384
NaeI GCCGGC 2 cut(s) 334, 714
NciI CCSGG 8 cut(s) 522, 527, 570, 627, 680, 681, 792, 838
NdeII GATC 4 cut(s) 71, 235, 348, 707
NgoMIV GCCGGC 2 cut(s) 332, 712
NlaIII CATG 1 cut(s) 911
NlaIV GGNNCC 3 cut(s) 206, 767, 855
NmeAIII GCCGAG 1 cut(s) 377
NmuCI GTSAC 1 cut(s) 674
NspI RCATGY 1 cut(s) 911
NspV TTCGAA 1 cut(s) 824
PciI ACATGT 1 cut(s) 907
PcsI WCGNNNNNNNCGW 4 cut(s) 114, 359, 716, 830
PctI GAATGC 2 cut(s) 601, 926
PdiI GCCGGC 2 cut(s) 334, 714
PflFI GACNNNGTC 1 cut(s) 661
PinAI ACCGGT 2 cut(s) 214, 283
PkrI GCNGC 5 cut(s) 96, 338, 377, 461, 464
PleI GAGTC 3 cut(s) 670, 807, 898
PpsI GAGTC 3 cut(s) 670, 807, 898
PpuMI RGGWCCY 1 cut(s) 204
PscI ACATGT 1 cut(s) 907
Psp124BI GAGCTC 2 cut(s) 91, 692
Psp5II RGGWCCY 1 cut(s) 204
PspEI GGTNACC 1 cut(s) 409
PspN4I GGNNCC 3 cut(s) 206, 767, 855
PspPI GGNCC 6 cut(s) 204, 523, 584, 702, 809, 879
PspPPI RGGWCCY 1 cut(s) 204
PstI CTGCAG 1 cut(s) 384
PsuI RGATCY 2 cut(s) 71, 348
PsyI GACNNNGTC 1 cut(s) 661
RsaI GTAC 1 cut(s) 581
RsaNI GTAC 1 cut(s) 580
Rsr2I CGGWCCG 1 cut(s) 584
RsrII CGGWCCG 1 cut(s) 584
SacI GAGCTC 2 cut(s) 91, 692
SalI GTCGAC 1 cut(s) 263
SaqAI TTAA 1 cut(s) 744
SatI GCNGC 5 cut(s) 95, 337, 376, 460, 463
Sau3AI GATC 4 cut(s) 71, 235, 348, 707
Sau96I GGNCC 6 cut(s) 204, 523, 584, 702, 809, 879
SchI GAGTC 3 cut(s) 671, 808, 899
ScrFI CCNGG 8 cut(s) 522, 527, 570, 627, 680, 681, 792, 838
SduI GDGCHC 4 cut(s) 91, 692, 770, 858
SfcI CTRYAG 1 cut(s) 380
SfuI TTCGAA 1 cut(s) 824
SgrAI CRCCGGYG 4 cut(s) 97, 332, 478, 712
SgrDI CGTCGACG 1 cut(s) 263
SinI GGWCC 4 cut(s) 204, 584, 702, 809
SmaI CCCGGG 1 cut(s) 681
Sse9I AATT 1 cut(s) 485
SspI AATATT 1 cut(s) 846
SstI GAGCTC 2 cut(s) 91, 692
StyD4I CCNGG 8 cut(s) 520, 525, 568, 625, 678, 679, 790, 836
TaaI ACNGT 4 cut(s) 118, 409, 584, 737
TaiI ACGT 2 cut(s) 270, 510
TaqI TCGA 5 cut(s) 264, 653, 665, 824, 833
TasI AATT 1 cut(s) 485
TauI GCSGC 2 cut(s) 97, 339
Tru1I TTAA 1 cut(s) 744
Tru9I TTAA 1 cut(s) 744
TscAI CASTG 1 cut(s) 189
TseFI GTSAC 1 cut(s) 674
TseI GCWGC 3 cut(s) 375, 459, 462
Tsp45I GTSAC 1 cut(s) 674
TspDTI ATGAA 1 cut(s) 916
TspGWI ACGGA 3 cut(s) 357, 572, 674
TspMI CCCGGG 1 cut(s) 679
TspRI CASTG 1 cut(s) 189
Tth111I GACNNNGTC 1 cut(s) 661
VpaK11BI GGWCC 4 cut(s) 204, 584, 702, 809
XceI RCATGY 1 cut(s) 911
XmaI CCCGGG 1 cut(s) 679
XmiI GTMKAC 1 cut(s) 264
ZraI GACGTC 2 cut(s) 268, 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.