pycom06g00550

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0010571
Physical Location & Seq
Reverse (-)
518418 .. 524239
5822 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1692 bp
ATGGCACCAGCAGCTGCGGGAGAAAGAAGTTTAAAGGAGACGCCAACATGGGCTGTTGCCATCGCCTGTGCAGTTTTCATAGTTATTTCTGTTATCATAGAACAAGCAATTCATGCTCTTGGAAAGTGGTTCCAGAAACGCAATAAGAAAGCTATGCTTGAAGCTTTGGAGAAAATAAAAGCTGAGCTAATGCTTTTGGGGTTCATATCTCTACTGCTTACTGTTGGAACACGATTTCTTCCAAAAATATGCATCTCAACCAAGTATGGTGATACCATGCTTCCATGTCAGTTAAACGAGTCCAAGTATAGTGACAAGGGCGGCAAGGATAAAGGTAATAAAGGAGGAGGCGACGATGATCGACGACGAAAGTTACTGTCACATGCTGAAAACATGGTCTGGCGTCGGGTTTTAGCCCCTAGTGCAGGAGATTCATCTTCTTGCGCAGGAGGTAAAGTACCATTGATATCTGCATCTGGGATGCACCAATTGCATATATTCATATTCGTGCTTGCTGTTTTTCATGTTTTCTACAGTGTCATCACTATGGCACTTGCGCAGGCCAAAATGAAGAAATGGAAAGCTTGGGAATCAGAGACATCGTCCTTGGAATATCAGTTTACAAATGATCCTTCAAGATTCAGGTTTACTCGACAAACTTCGTTTGTGAAGCGTCATTCTGGCTTTTCTACAATGCCTGGAATAAGATGGATTGTGGCATTCTTCAGGCAATTCTTTGGTTCAGTAACACGGGTGGACTATTTGACAATGCGCCACGGATTTATTAATGCACATTTTGCTCCAAATAGCAAATTTGACTTCCATAAGTATATTAAGAGATCCATGGAAGATGACTTTAAGGTCGTCGTGGGAATCAGTTTACCACTATGGATTTTTGCCGTCGTTTTTTTGCTTCTAAACGTTTACAAATGGTACACGCTCACTTGGTTGTCGTTGGCTCCTCTCATTATTCTTCTTCTGGTAGGTACAAAGCTTGAGCTTATCATCGTGGAGATGGCTCAAGAAATGCATGATCGGAATACAGTTGTTAAAGGCGCTCCCGTTGTGGAGCCAAACAACAAGTACTTTTGGTTTAACCGACCTGAGTGGACTCTTCTCTTAATTCATTTTACATTATTTCAGAACGCATTCCAAATGGCGTACTTCGTCTGGATATCTTCTGAATTTGGGTTCACATCATGCTTCCACGACAACTTGCCTCTGATATTGATAAGGGTTTTCCTTGGGGTAGCCCTACAGGTCCTATGCAGTTACATTACCTTCCCTCTTTATTGTTTGATTACACAAATGGGATCTCATATGAAGAAAGCAATTTTTGAGGAGCAAACGGCAAAGGCTCTTAAGAACTGGCAAAAGGCTGCAAAGCAAAGAAAGAAGTTGAGGAATACTAAAGGAGATGCTTCTTCCTCAGGATTTGTAAGCGGGGAAAATACCCCAAGTCGGGGTACATCACCCTTGCATCTGCTTCACAACCAAAAGCTCAGGTCCAATCAATCCGACCTTGAAAGCGTCCTCAATTCCCCAAGATCTTACCAATCTGACGCCGAGCTCTCGGAGATGGAAGGCTCCGCACATGAAGGGAATGGTTTTAGAAGACTAGGAGACCAACCTGCAACAGCAATAGAAATGAAAGAAGGATCACATAATGTTGATTTTACATTTGCTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

564

Amino Acids

63.77

Weight (kDa)

9.53

Isoelectric Point (pI)

43.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 8 - 499 7.1e-228 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 860
Acc16I TGCGCA 2 cut(s) 445, 558
Acc36I ACCTGC 1 cut(s) 1639
AccB1I GGYRCC 1 cut(s) 4
AciI CCGC 4 cut(s) 17, 321, 1443, 1590
AclI AACGTT 1 cut(s) 921
AclWI GGATC 4 cut(s) 623, 834, 1321, 1666
AcsI RAATTY 2 cut(s) 812, 1184
AcuI CTGAAG 1 cut(s) 709
AcyI GRCGYC 3 cut(s) 41, 403, 1563
AfaI GTAC 6 cut(s) 459, 935, 988, 1085, 1163, 1468
AfiI CCNNNNNNNGG 4 cut(s) 425, 1461, 1462, 1463
AflII CTTAAG 1 cut(s) 1361
AgsI TTSAA 3 cut(s) 161, 636, 1526
AjnI CCWGG 1 cut(s) 697
Alw21I GWGCWC 1 cut(s) 1572
Alw26I GTCTC 3 cut(s) 32, 590, 1617
AlwI GGATC 4 cut(s) 623, 834, 1321, 1666
AlwNI CAGNNNCTG 1 cut(s) 14
AoxI GGCC 1 cut(s) 561
ApeKI GCWGC 3 cut(s) 11, 14, 1379
ApoI RAATTY 2 cut(s) 812, 1184
AseI ATTAAT 1 cut(s) 786
Asp700I GAANNNNTTC 1 cut(s) 1148
AspLEI GCGC 4 cut(s) 446, 559, 774, 1058
AspS9I GGNCC 2 cut(s) 1261, 1506
AsuHPI GGTGA 2 cut(s) 281, 1464
AvaII GGWCC 2 cut(s) 1261, 1506
AxyI CCTNAGG 1 cut(s) 1429
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 1572
BbsI GAAGAC 1 cut(s) 1621
Bbv12I GWGCWC 1 cut(s) 1572
BbvI GCAGC 2 cut(s) 23, 1366
BccI CCATC 4 cut(s) 68, 702, 1009, 1573
BceAI ACGGC 2 cut(s) 884, 1365
BciT130I CCWGG 1 cut(s) 699
BcoDI GTCTC 3 cut(s) 32, 590, 1617
BfaI CTAG 3 cut(s) 420, 1619, 1686
BfmI CTRYAG 2 cut(s) 532, 1256
BfoI RGCGCY 1 cut(s) 1059
BfrI CTTAAG 1 cut(s) 1361
BfuAI ACCTGC 1 cut(s) 1639
BglII AGATCT 1 cut(s) 1547
BisI GCNGC 4 cut(s) 12, 15, 322, 1380
BlpI GCTNAGC 1 cut(s) 183
BlsI GCNGC 4 cut(s) 13, 16, 323, 1381
BmcAI AGTACT 1 cut(s) 1085
Bme1390I CCNGG 1 cut(s) 699
Bme18I GGWCC 2 cut(s) 1261, 1506
BmgT120I GGNCC 2 cut(s) 1261, 1506
BmiI GGNNCC 5 cut(s) 6, 131, 960, 1071, 1588
BmrFI CCNGG 1 cut(s) 699
BmsI GCATC 5 cut(s) 261, 471, 482, 1408, 1489
BpiI GAAGAC 1 cut(s) 1621
Bpu10I CCTNAGC 1 cut(s) 1502
Bpu1102I GCTNAGC 1 cut(s) 183
BpuEI CTTGAG 2 cut(s) 1005, 1016
BsaHI GRCGYC 3 cut(s) 41, 403, 1563
BsaI GGTCTC 1 cut(s) 1617
BsaJI CCNNGG 4 cut(s) 606, 775, 843, 1243
BsaXI ACNNNNNCTCC 2 cut(s) 441, 471
Bsc4I CCNNNNNNNGG 4 cut(s) 425, 1461, 1462, 1463
Bse1I ACTGG 1 cut(s) 1373
Bse21I CCTNAGG 1 cut(s) 1429
BseBI CCWGG 1 cut(s) 699
BseDI CCNNGG 4 cut(s) 606, 775, 843, 1243
BseGI GGATG 1 cut(s) 486
BseLI CCNNNNNNNGG 4 cut(s) 425, 1461, 1462, 1463
BseMII CTCAG 4 cut(s) 174, 1095, 1443, 1516
BseNI ACTGG 1 cut(s) 1373
BseRI GAGGAG 3 cut(s) 360, 951, 1355
BseXI GCAGC 2 cut(s) 23, 1366
BsgI GTGCAG 2 cut(s) 90, 444
BshFI GGCC 1 cut(s) 563
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 1 cut(s) 1572
BslI CCNNNNNNNGG 4 cut(s) 425, 1461, 1462, 1463
BsmAI GTCTC 3 cut(s) 32, 590, 1617
BsmBI CGTCTC 1 cut(s) 32
BsmI GAATGC 2 cut(s) 719, 1148
BsnI GGCC 1 cut(s) 563
Bso31I GGTCTC 1 cut(s) 1617
Bsp1286I GDGCHC 1 cut(s) 1572
Bsp143I GATC 7 cut(s) 358, 628, 839, 1033, 1313, 1547, 1658
Bsp1720I GCTNAGC 1 cut(s) 183
Bsp19I CCATGG 1 cut(s) 843
BspACI CCGC 4 cut(s) 17, 321, 1443, 1590
BspANI GGCC 1 cut(s) 563
BspCNI CTCAG 4 cut(s) 175, 1096, 1442, 1515
BspLI GGNNCC 5 cut(s) 6, 131, 960, 1071, 1588
BspMI ACCTGC 1 cut(s) 1639
BspPI GGATC 4 cut(s) 623, 834, 1321, 1666
BspT107I GGYRCC 1 cut(s) 4
BspTI CTTAAG 1 cut(s) 1361
BspTNI GGTCTC 1 cut(s) 1617
BsrI ACTGG 1 cut(s) 1373
BssECI CCNNGG 4 cut(s) 606, 775, 843, 1243
BssMI GATC 7 cut(s) 358, 628, 839, 1033, 1313, 1547, 1658
BssNI GRCGYC 3 cut(s) 41, 403, 1563
BssT1I CCWWGG 3 cut(s) 606, 843, 1243
Bst2UI CCWGG 1 cut(s) 699
Bst4CI ACNGT 4 cut(s) 223, 378, 536, 1045
Bst6I CTCTTC 1 cut(s) 1119
BstACI GRCGYC 3 cut(s) 41, 403, 1563
BstAFI CTTAAG 1 cut(s) 1361
BstAPI GCANNNNNTGC 3 cut(s) 113, 490, 797
BstC8I GCNNGC 2 cut(s) 513, 561
BstDEI CTNAG 4 cut(s) 183, 1104, 1429, 1502
BstDSI CCRYGG 2 cut(s) 775, 843
BstENI CCTNNNNNAGG 1 cut(s) 423
BstF5I GGATG 1 cut(s) 486
BstH2I RGCGCY 1 cut(s) 1059
BstHHI GCGC 4 cut(s) 446, 559, 774, 1058
BstKTI GATC 7 cut(s) 361, 631, 842, 1036, 1316, 1550, 1661
BstMAI GTCTC 3 cut(s) 32, 590, 1617
BstMBI GATC 7 cut(s) 358, 628, 839, 1033, 1313, 1547, 1658
BstMWI GCNNNNNNNGC 5 cut(s) 11, 113, 422, 490, 797
BstNI CCWGG 1 cut(s) 699
BstNSI RCATGY 1 cut(s) 386
BstSCI CCNGG 1 cut(s) 697
BstSFI CTRYAG 2 cut(s) 532, 1256
BstV1I GCAGC 2 cut(s) 23, 1366
BstV2I GAAGAC 1 cut(s) 1621
BstX2I RGATCY 3 cut(s) 839, 1313, 1547
BstYI RGATCY 3 cut(s) 839, 1313, 1547
Bsu36I CCTNAGG 1 cut(s) 1429
BsuRI GGCC 1 cut(s) 563
BtgI CCRYGG 2 cut(s) 775, 843
BtgZI GCGATG 1 cut(s) 46
BtsCI GGATG 1 cut(s) 486
BtsIMutI CAGTG 1 cut(s) 541
BveI ACCTGC 1 cut(s) 1639
Cac8I GCNNGC 2 cut(s) 513, 561
CaiI CAGNNNCTG 1 cut(s) 14
CfoI GCGC 4 cut(s) 446, 559, 774, 1058
Cfr13I GGNCC 2 cut(s) 1261, 1506
CseI GACGC 5 cut(s) 49, 392, 662, 1519, 1571
Csp6I GTAC 6 cut(s) 458, 934, 987, 1084, 1162, 1467
CviQI GTAC 6 cut(s) 458, 934, 987, 1084, 1162, 1467
DdeI CTNAG 4 cut(s) 183, 1104, 1429, 1502
DpnI GATC 7 cut(s) 360, 630, 841, 1035, 1315, 1549, 1660
DpnII GATC 7 cut(s) 358, 628, 839, 1033, 1313, 1547, 1658
DraI TTTAAA 1 cut(s) 33
DrdI GACNNNNNNGTC 1 cut(s) 860
DseDI GACNNNNNNGTC 1 cut(s) 860
Eam1104I CTCTTC 1 cut(s) 1119
EarI CTCTTC 1 cut(s) 1119
Ecl136II GAGCTC 1 cut(s) 1570
Eco130I CCWWGG 3 cut(s) 606, 843, 1243
Eco24I GRGCYC 1 cut(s) 1572
Eco31I GGTCTC 1 cut(s) 1617
Eco32I GATATC 2 cut(s) 468, 1176
Eco47I GGWCC 2 cut(s) 1261, 1506
Eco53kI GAGCTC 1 cut(s) 1570
Eco57I CTGAAG 1 cut(s) 709
Eco81I CCTNAGG 1 cut(s) 1429
EcoICRI GAGCTC 1 cut(s) 1570
EcoNI CCTNNNNNAGG 1 cut(s) 423
EcoO109I RGGNCCY 1 cut(s) 1261
EcoRII CCWGG 1 cut(s) 697
EcoRV GATATC 2 cut(s) 468, 1176
EcoT14I CCWWGG 3 cut(s) 606, 843, 1243
EcoT22I ATGCAT 2 cut(s) 254, 1032
EcoT38I GRGCYC 1 cut(s) 1572
ErhI CCWWGG 3 cut(s) 606, 843, 1243
Esp3I CGTCTC 1 cut(s) 32
FalI AAGNNNNNCTT 2 cut(s) 141, 173
FauI CCCGC 2 cut(s) 10, 1436
FauNDI CATATG 1 cut(s) 1320
Fnu4HI GCNGC 4 cut(s) 12, 15, 322, 1380
FokI GGATG 1 cut(s) 493
FriOI GRGCYC 1 cut(s) 1572
Fsp4HI GCNGC 4 cut(s) 12, 15, 322, 1380
FspBI CTAG 3 cut(s) 420, 1619, 1686
FspI TGCGCA 2 cut(s) 445, 558
GlaI GCGC 4 cut(s) 445, 558, 773, 1057
GluI GCNGC 4 cut(s) 12, 15, 322, 1380
HaeII RGCGCY 1 cut(s) 1059
HaeIII GGCC 1 cut(s) 563
HgaI GACGC 5 cut(s) 49, 392, 662, 1519, 1571
HhaI GCGC 4 cut(s) 446, 559, 774, 1058
Hin1I GRCGYC 3 cut(s) 41, 403, 1563
Hin6I GCGC 4 cut(s) 444, 557, 772, 1056
HinP1I GCGC 4 cut(s) 444, 557, 772, 1056
HindIII AAGCTT 3 cut(s) 162, 582, 992
HinfI GANTC 6 cut(s) 299, 431, 590, 639, 873, 1111
HphI GGTGA 2 cut(s) 281, 1464
Hpy166II GTNNAC 8 cut(s) 621, 648, 757, 881, 925, 936, 1110, 1194
Hpy188I TCNGA 8 cut(s) 595, 1038, 1143, 1183, 1224, 1519, 1561, 1576
Hpy188III TCNNGA 5 cut(s) 133, 636, 1022, 1171, 1431
Hpy8I GTNNAC 8 cut(s) 621, 648, 757, 881, 925, 936, 1110, 1194
Hpy99I CGWCG 6 cut(s) 356, 366, 369, 408, 869, 905
HpyAV CCTTC 5 cut(s) 642, 1291, 1577, 1592, 1649
HpyCH4III ACNGT 4 cut(s) 223, 378, 536, 1045
HpyCH4IV ACGT 1 cut(s) 921
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 113, 422, 490, 797
HpyF3I CTNAG 4 cut(s) 183, 1104, 1429, 1502
HpySE526I ACGT 1 cut(s) 921
Hsp92I GRCGYC 3 cut(s) 41, 403, 1563
HspAI GCGC 4 cut(s) 444, 557, 772, 1056
Kzo9I GATC 7 cut(s) 358, 628, 839, 1033, 1313, 1547, 1658
LmnI GCTCC 6 cut(s) 805, 964, 1063, 1069, 1342, 1592
Lsp1109I GCAGC 2 cut(s) 23, 1366
LweI GCATC 5 cut(s) 261, 471, 482, 1408, 1489
MaeI CTAG 3 cut(s) 420, 1619, 1686
MaeII ACGT 1 cut(s) 921
MaeIII GTNAC 5 cut(s) 311, 372, 378, 745, 1271
MalI GATC 7 cut(s) 360, 630, 841, 1035, 1315, 1549, 1660
MboI GATC 7 cut(s) 358, 628, 839, 1033, 1313, 1547, 1658
MfeI CAATTG 1 cut(s) 488
MflI RGATCY 3 cut(s) 839, 1313, 1547
MhlI GDGCHC 1 cut(s) 1572
MluCI AATT 8 cut(s) 108, 488, 731, 812, 1122, 1184, 1332, 1537
MlyI GAGTC 2 cut(s) 308, 1105
MmeI TCCRAC 2 cut(s) 205, 1542
Mph1103I ATGCAT 2 cut(s) 254, 1032
MroXI GAANNNNTTC 1 cut(s) 1148
MseI TTAA 9 cut(s) 32, 293, 786, 834, 858, 1050, 1095, 1121, 1362
MslI CAYNNNNRTG 2 cut(s) 506, 545
MspA1I CMGCKG 1 cut(s) 14
MspCI CTTAAG 1 cut(s) 1361
MspR9I CCNGG 1 cut(s) 699
MunI CAATTG 1 cut(s) 488
Mva1269I GAATGC 2 cut(s) 719, 1148
MvaI CCWGG 1 cut(s) 699
MwoI GCNNNNNNNGC 5 cut(s) 11, 113, 422, 490, 797
NcoI CCATGG 1 cut(s) 843
NdeI CATATG 1 cut(s) 1320
NdeII GATC 7 cut(s) 358, 628, 839, 1033, 1313, 1547, 1658
NlaIV GGNNCC 5 cut(s) 6, 131, 960, 1071, 1588
NmeAIII GCCGAG 1 cut(s) 1591
NmuCI GTSAC 2 cut(s) 311, 378
NsbI TGCGCA 2 cut(s) 445, 558
NsiI ATGCAT 2 cut(s) 254, 1032
NspI RCATGY 1 cut(s) 386
PctI GAATGC 2 cut(s) 719, 1148
PdmI GAANNNNTTC 1 cut(s) 1148
PfeI GAWTC 4 cut(s) 431, 590, 639, 873
PflFI GACNNNGTC 1 cut(s) 601
PkrI GCNGC 4 cut(s) 13, 16, 323, 1381
PleI GAGTC 2 cut(s) 307, 1105
PpsI GAGTC 2 cut(s) 307, 1105
PpuMI RGGWCCY 1 cut(s) 1261
PshBI ATTAAT 1 cut(s) 786
Psp124BI GAGCTC 1 cut(s) 1572
Psp1406I AACGTT 1 cut(s) 921
Psp5II RGGWCCY 1 cut(s) 1261
Psp6I CCWGG 1 cut(s) 697
PspGI CCWGG 1 cut(s) 697
PspN4I GGNNCC 5 cut(s) 6, 131, 960, 1071, 1588
PspPI GGNCC 2 cut(s) 1261, 1506
PspPPI RGGWCCY 1 cut(s) 1261
PstNI CAGNNNCTG 1 cut(s) 14
PsuI RGATCY 3 cut(s) 839, 1313, 1547
PsyI GACNNNGTC 1 cut(s) 601
PvuII CAGCTG 1 cut(s) 14
RsaI GTAC 6 cut(s) 459, 935, 988, 1085, 1163, 1468
RsaNI GTAC 6 cut(s) 458, 934, 987, 1084, 1162, 1467
RseI CAYNNNNRTG 2 cut(s) 506, 545
SacI GAGCTC 1 cut(s) 1572
SaqAI TTAA 9 cut(s) 32, 293, 786, 834, 858, 1050, 1095, 1121, 1362
SatI GCNGC 4 cut(s) 12, 15, 322, 1380
Sau3AI GATC 7 cut(s) 358, 628, 839, 1033, 1313, 1547, 1658
Sau96I GGNCC 2 cut(s) 1261, 1506
ScaI AGTACT 1 cut(s) 1085
SchI GAGTC 2 cut(s) 308, 1105
ScrFI CCNGG 1 cut(s) 699
SduI GDGCHC 1 cut(s) 1572
SfaNI GCATC 5 cut(s) 261, 471, 482, 1408, 1489
SfcI CTRYAG 2 cut(s) 532, 1256
SinI GGWCC 2 cut(s) 1261, 1506
SmiMI CAYNNNNRTG 2 cut(s) 506, 545
SmlI CTYRAG 3 cut(s) 995, 1020, 1361
SmoI CTYRAG 3 cut(s) 995, 1020, 1361
Sse9I AATT 8 cut(s) 108, 488, 731, 812, 1122, 1184, 1332, 1537
SsiI CCGC 4 cut(s) 17, 321, 1443, 1590
SspMI CTAG 3 cut(s) 420, 1619, 1686
SstI GAGCTC 1 cut(s) 1572
StyD4I CCNGG 1 cut(s) 697
StyI CCWWGG 3 cut(s) 606, 843, 1243
TaaI ACNGT 4 cut(s) 223, 378, 536, 1045
TaiI ACGT 1 cut(s) 924
TaqI TCGA 2 cut(s) 361, 652
TasI AATT 8 cut(s) 108, 488, 731, 812, 1122, 1184, 1332, 1537
TatI WGTACW 1 cut(s) 1083
TauI GCSGC 1 cut(s) 324
TfiI GAWTC 4 cut(s) 431, 590, 639, 873
Tru1I TTAA 9 cut(s) 32, 293, 786, 834, 858, 1050, 1095, 1121, 1362
Tru9I TTAA 9 cut(s) 32, 293, 786, 834, 858, 1050, 1095, 1121, 1362
TscAI CASTG 1 cut(s) 541
TseFI GTSAC 2 cut(s) 311, 378
TseI GCWGC 3 cut(s) 11, 14, 1379
Tsp45I GTSAC 2 cut(s) 311, 378
TspGWI ACGGA 1 cut(s) 792
TspRI CASTG 1 cut(s) 541
Tth111I GACNNNGTC 1 cut(s) 601
Vha464I CTTAAG 1 cut(s) 1361
VpaK11BI GGWCC 2 cut(s) 1261, 1506
VspI ATTAAT 1 cut(s) 786
XagI CCTNNNNNAGG 1 cut(s) 423
XapI RAATTY 2 cut(s) 812, 1184
XceI RCATGY 1 cut(s) 386
XmnI GAANNNNTTC 1 cut(s) 1148
XspI CTAG 3 cut(s) 420, 1619, 1686
ZrmI AGTACT 1 cut(s) 1085
Zsp2I ATGCAT 2 cut(s) 254, 1032
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.