pycom06g03020

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0010290
Physical Location & Seq
Forward (+)
3812554 .. 3814069
1516 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 741 bp
ATGATCCGAACCGGTTCTTTTGCGCTCCGGTCACCGGCCGTTTCCGGCTCGCCTAGAGTGTCCCTAGCCCGGAACGACTCGCTCTCGAGCGTTTTCTCCGGCGAGAGGAGCACCGTCGGATCTCCCATGATCTCCCTGCATTTCGACGTCAATCGCCGCCGAGATAAGATGATCCGGAGATCTCTGTCGGAGAGCGACGTTCTGCAATCGGCGTCCGACGGATTTCGAGCACTGTCGGCGAGTTACGCCGGTAAATTGGCGGGAAGTGGAATTTCGACGGAGAAGCTGGTTCCTGGCGGCGGCGATTGCGGAGGGAATACTGTAACGGTGCAGGAAGGCGAGCAGAGGAAGATCGGAGCGTATTATCAAGAGATGCTGAAGTCAAACCCCGGCGATCCGTTGCTGCTGAGGAACTACGGGAAATTCTTGCACGAGGTGGAGAAGGATGCGGTGGGGGCGGAGGAGTGCTATTGTAGGGCGATATTGGAGAGTCCGGGAGACGGTGATTTGTTGTCGCTCTACGGAAAGCTAATCTGGGAGACGCAGAGAGATGAGGAACGGGCTAAATCTTACTTTGATCAGGCTGTTTCTGCCTCCCCTGACGATTGCATGGTGTTGGGGTCCTTCGCTAGCTTCATGTGGGAAGCTGAGGAAGACGAAGACGAAGACGACAATGAAGAAATCAACATTGACGACGGAACTCAAGCCTCATCTGTGCCGGCTCTGGTTCCTGCATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

247

Amino Acids

26.69

Weight (kDa)

4.56

Isoelectric Point (pI)

58.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_TmcB PF25474 117 - 160 4.4e-06 TmcB, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 150
AccIII TCCGGA 1 cut(s) 174
AciI CCGC 7 cut(s) 157, 260, 297, 300, 309, 449, 458
AclWI GGATC 3 cut(s) 127, 166, 389
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 2 cut(s) 270, 422
AcuI CTGAAG 1 cut(s) 398
AcyI GRCGYC 2 cut(s) 147, 212
AdeI CACNNNGTG 1 cut(s) 436
AfiI CCNNNNNNNGG 5 cut(s) 34, 69, 105, 299, 500
AgeI ACCGGT 1 cut(s) 11
AjnI CCWGG 1 cut(s) 292
AluBI AGCT 4 cut(s) 286, 529, 633, 647
AluI AGCT 4 cut(s) 286, 529, 633, 647
Alw21I GWGCWC 2 cut(s) 113, 232
Alw26I GTCTC 2 cut(s) 492, 533
AlwI GGATC 3 cut(s) 127, 166, 389
Ama87I CYCGRG 1 cut(s) 85
Aor13HI TCCGGA 1 cut(s) 174
AoxI GGCC 1 cut(s) 36
ApeKI GCWGC 1 cut(s) 403
ApoI RAATTY 2 cut(s) 270, 422
AsiGI ACCGGT 1 cut(s) 11
Asp700I GAANNNNTTC 1 cut(s) 13
AspLEI GCGC 1 cut(s) 25
AspS9I GGNCC 1 cut(s) 621
AsuC2I CCSGG 3 cut(s) 70, 390, 495
AsuHPI GGTGA 2 cut(s) 24, 515
AsuNHI GCTAGC 1 cut(s) 629
AvaI CYCGRG 1 cut(s) 85
AvaII GGWCC 1 cut(s) 621
BauI CACGAG 1 cut(s) 431
BbsI GAAGAC 3 cut(s) 660, 666, 672
Bbv12I GWGCWC 2 cut(s) 113, 232
BbvCI CCTCAGC 2 cut(s) 407, 648
BbvI GCAGC 1 cut(s) 390
BceAI ACGGC 1 cut(s) 23
BciT130I CCWGG 1 cut(s) 294
BclI TGATCA 1 cut(s) 577
BcnI CCSGG 3 cut(s) 70, 390, 495
BcoDI GTCTC 2 cut(s) 492, 533
BfaI CTAG 3 cut(s) 54, 65, 630
BglII AGATCT 1 cut(s) 179
BisI GCNGC 4 cut(s) 157, 298, 301, 404
BlsI GCNGC 4 cut(s) 158, 299, 302, 405
Bme1390I CCNGG 4 cut(s) 70, 294, 390, 495
Bme18I GGWCC 1 cut(s) 621
BmeT110I CYCGRG 1 cut(s) 85
BmgT120I GGNCC 1 cut(s) 621
BmiI GGNNCC 3 cut(s) 291, 622, 729
BmrFI CCNGG 4 cut(s) 70, 294, 390, 495
BmsI GCATC 2 cut(s) 363, 436
BmtI GCTAGC 1 cut(s) 633
BpiI GAAGAC 3 cut(s) 660, 666, 672
Bpu10I CCTNAGC 2 cut(s) 407, 648
BpuEI CTTGAG 1 cut(s) 687
BpuMI CCSGG 3 cut(s) 70, 390, 495
BsaHI GRCGYC 2 cut(s) 147, 212
BsaJI CCNNGG 1 cut(s) 388
BsaWI WCCGGW 3 cut(s) 11, 27, 174
Bsc4I CCNNNNNNNGG 5 cut(s) 34, 69, 105, 299, 500
Bse118I RCCGGY 4 cut(s) 11, 34, 248, 718
BseAI TCCGGA 1 cut(s) 174
BseBI CCWGG 1 cut(s) 294
BseDI CCNNGG 1 cut(s) 388
BseGI GGATG 1 cut(s) 451
BseLI CCNNNNNNNGG 5 cut(s) 34, 69, 105, 299, 500
BseMII CTCAG 2 cut(s) 398, 639
BseRI GAGGAG 2 cut(s) 121, 476
BseX3I CGGCCG 1 cut(s) 36
BseXI GCAGC 1 cut(s) 390
BsgI GTGCAG 1 cut(s) 350
Bsh1285I CGRYCG 1 cut(s) 39
BshFI GGCC 1 cut(s) 38
BshTI ACCGGT 1 cut(s) 11
BsiEI CGRYCG 1 cut(s) 39
BsiHKAI GWGCWC 2 cut(s) 113, 232
BsiHKCI CYCGRG 1 cut(s) 85
BslFI GGGAC 1 cut(s) 46
BslI CCNNNNNNNGG 5 cut(s) 34, 69, 105, 299, 500
BsmAI GTCTC 2 cut(s) 492, 533
BsmBI CGTCTC 2 cut(s) 492, 533
BsmFI GGGAC 1 cut(s) 46
BsnI GGCC 1 cut(s) 38
BsoBI CYCGRG 1 cut(s) 85
Bsp1286I GDGCHC 2 cut(s) 113, 232
Bsp13I TCCGGA 1 cut(s) 174
Bsp143I GATC 8 cut(s) 3, 119, 129, 171, 179, 351, 394, 577
BspACI CCGC 7 cut(s) 157, 260, 297, 300, 309, 449, 458
BspANI GGCC 1 cut(s) 38
BspCNI CTCAG 2 cut(s) 399, 640
BspEI TCCGGA 1 cut(s) 174
BspLI GGNNCC 3 cut(s) 291, 622, 729
BspOI GCTAGC 1 cut(s) 633
BspPI GGATC 3 cut(s) 127, 166, 389
BsrFI RCCGGY 4 cut(s) 11, 34, 248, 718
BssAI RCCGGY 4 cut(s) 11, 34, 248, 718
BssECI CCNNGG 1 cut(s) 388
BssMI GATC 8 cut(s) 3, 119, 129, 171, 179, 351, 394, 577
BssNI GRCGYC 2 cut(s) 147, 212
BssSI CACGAG 1 cut(s) 431
Bst2BI CACGAG 1 cut(s) 431
Bst2UI CCWGG 1 cut(s) 294
Bst4CI ACNGT 5 cut(s) 115, 234, 322, 328, 503
BstACI GRCGYC 2 cut(s) 147, 212
BstC8I GCNNGC 4 cut(s) 50, 341, 631, 720
BstDEI CTNAG 2 cut(s) 407, 648
BstEII GGTNACC 1 cut(s) 30
BstF5I GGATG 1 cut(s) 451
BstHHI GCGC 1 cut(s) 25
BstKTI GATC 8 cut(s) 6, 122, 132, 174, 182, 354, 397, 580
BstMAI GTCTC 2 cut(s) 492, 533
BstMBI GATC 8 cut(s) 3, 119, 129, 171, 179, 351, 394, 577
BstMCI CGRYCG 1 cut(s) 39
BstMWI GCNNNNNNNGC 6 cut(s) 108, 236, 245, 306, 455, 590
BstNI CCWGG 1 cut(s) 294
BstPI GGTNACC 1 cut(s) 30
BstSCI CCNGG 4 cut(s) 68, 292, 388, 493
BstV1I GCAGC 1 cut(s) 390
BstV2I GAAGAC 3 cut(s) 660, 666, 672
BstX2I RGATCY 2 cut(s) 119, 179
BstYI RGATCY 2 cut(s) 119, 179
BstZI CGGCCG 1 cut(s) 36
BsuRI GGCC 1 cut(s) 38
BtsCI GGATG 1 cut(s) 451
BtsIMutI CAGTG 1 cut(s) 230
Cac8I GCNNGC 4 cut(s) 50, 341, 631, 720
CfoI GCGC 1 cut(s) 25
Cfr10I RCCGGY 4 cut(s) 11, 34, 248, 718
Cfr13I GGNCC 1 cut(s) 621
CseI GACGC 2 cut(s) 201, 550
CspAI ACCGGT 1 cut(s) 11
CviAII CATG 3 cut(s) 127, 610, 637
DdeI CTNAG 2 cut(s) 407, 648
DpnI GATC 8 cut(s) 5, 121, 131, 173, 181, 353, 396, 579
DpnII GATC 8 cut(s) 3, 119, 129, 171, 179, 351, 394, 577
DraIII CACNNNGTG 1 cut(s) 436
EaeI YGGCCR 1 cut(s) 36
EagI CGGCCG 1 cut(s) 36
EciI GGCGGA 1 cut(s) 473
EclXI CGGCCG 1 cut(s) 36
Eco47I GGWCC 1 cut(s) 621
Eco52I CGGCCG 1 cut(s) 36
Eco57I CTGAAG 1 cut(s) 398
Eco88I CYCGRG 1 cut(s) 85
Eco91I GGTNACC 1 cut(s) 30
EcoO109I RGGNCCY 1 cut(s) 621
EcoO65I GGTNACC 1 cut(s) 30
EcoRII CCWGG 1 cut(s) 292
Esp3I CGTCTC 2 cut(s) 492, 533
FaeI CATG 3 cut(s) 130, 613, 640
FaiI YATR 3 cut(s) 128, 611, 638
FaqI GGGAC 1 cut(s) 46
FatI CATG 3 cut(s) 126, 609, 636
FauI CCCGC 1 cut(s) 253
FbaI TGATCA 1 cut(s) 577
Fnu4HI GCNGC 4 cut(s) 157, 298, 301, 404
FokI GGATG 1 cut(s) 458
Fsp4HI GCNGC 4 cut(s) 157, 298, 301, 404
FspBI CTAG 3 cut(s) 54, 65, 630
GlaI GCGC 1 cut(s) 24
GluI GCNGC 4 cut(s) 157, 298, 301, 404
HaeIII GGCC 1 cut(s) 38
HgaI GACGC 2 cut(s) 201, 550
HhaI GCGC 1 cut(s) 25
Hin1I GRCGYC 2 cut(s) 147, 212
Hin1II CATG 3 cut(s) 130, 613, 640
Hin6I GCGC 1 cut(s) 23
HinP1I GCGC 1 cut(s) 23
HinfI GANTC 2 cut(s) 77, 490
HphI GGTGA 2 cut(s) 24, 515
Hpy188I TCNGA 5 cut(s) 8, 119, 190, 217, 356
Hpy188III TCNNGA 3 cut(s) 85, 175, 368
Hpy99I CGWCG 6 cut(s) 119, 149, 200, 221, 280, 698
HpyAV CCTTC 3 cut(s) 329, 436, 634
HpyCH4III ACNGT 5 cut(s) 115, 234, 322, 328, 503
HpyCH4IV ACGT 2 cut(s) 147, 198
HpyCH4V TGCA 6 cut(s) 139, 205, 331, 430, 609, 734
HpyF10VI GCNNNNNNNGC 6 cut(s) 108, 236, 245, 306, 455, 590
HpyF3I CTNAG 2 cut(s) 407, 648
HpySE526I ACGT 2 cut(s) 147, 198
Hsp92I GRCGYC 2 cut(s) 147, 212
Hsp92II CATG 3 cut(s) 130, 613, 640
HspAI GCGC 1 cut(s) 23
Kpn2I TCCGGA 1 cut(s) 174
KroI GCCGGC 1 cut(s) 718
KroNI GCCGGC 1 cut(s) 720
Ksp22I TGATCA 1 cut(s) 577
Kzo9I GATC 8 cut(s) 3, 119, 129, 171, 179, 351, 394, 577
LmnI GCTCC 3 cut(s) 30, 108, 356
Lsp1109I GCAGC 1 cut(s) 390
LweI GCATC 2 cut(s) 363, 436
MaeI CTAG 3 cut(s) 54, 65, 630
MaeII ACGT 2 cut(s) 147, 198
MaeIII GTNAC 3 cut(s) 30, 242, 322
MalI GATC 8 cut(s) 5, 121, 131, 173, 181, 353, 396, 579
MboI GATC 8 cut(s) 3, 119, 129, 171, 179, 351, 394, 577
MboII GAAGA 5 cut(s) 361, 665, 671, 677, 689
MflI RGATCY 2 cut(s) 119, 179
MhlI GDGCHC 2 cut(s) 113, 232
MluCI AATT 3 cut(s) 254, 270, 422
MlyI GAGTC 2 cut(s) 71, 499
MmeI TCCRAC 3 cut(s) 97, 168, 240
MroI TCCGGA 1 cut(s) 174
MroNI GCCGGC 1 cut(s) 718
MroXI GAANNNNTTC 1 cut(s) 13
MseI TTAA 1 cut(s) 739
MspR9I CCNGG 4 cut(s) 70, 294, 390, 495
MvaI CCWGG 1 cut(s) 294
MwoI GCNNNNNNNGC 6 cut(s) 108, 236, 245, 306, 455, 590
NaeI GCCGGC 1 cut(s) 720
NciI CCSGG 3 cut(s) 70, 390, 495
NdeII GATC 8 cut(s) 3, 119, 129, 171, 179, 351, 394, 577
NgoMIV GCCGGC 1 cut(s) 718
NheI GCTAGC 1 cut(s) 629
NlaIII CATG 3 cut(s) 130, 613, 640
NlaIV GGNNCC 3 cut(s) 291, 622, 729
NmeAIII GCCGAG 1 cut(s) 185
NmuCI GTSAC 1 cut(s) 30
PaeR7I CTCGAG 1 cut(s) 85
PdiI GCCGGC 1 cut(s) 720
PdmI GAANNNNTTC 1 cut(s) 13
PfoI TCCNGGA 1 cut(s) 493
PinAI ACCGGT 1 cut(s) 11
PkrI GCNGC 4 cut(s) 158, 299, 302, 405
PleI GAGTC 2 cut(s) 71, 498
PpsI GAGTC 2 cut(s) 71, 498
PpuMI RGGWCCY 1 cut(s) 621
Psp5II RGGWCCY 1 cut(s) 621
Psp6I CCWGG 1 cut(s) 292
PspEI GGTNACC 1 cut(s) 30
PspGI CCWGG 1 cut(s) 292
PspN4I GGNNCC 3 cut(s) 291, 622, 729
PspPI GGNCC 1 cut(s) 621
PspPPI RGGWCCY 1 cut(s) 621
PsuI RGATCY 2 cut(s) 119, 179
SaqAI TTAA 1 cut(s) 739
SatI GCNGC 4 cut(s) 157, 298, 301, 404
Sau3AI GATC 8 cut(s) 3, 119, 129, 171, 179, 351, 394, 577
Sau96I GGNCC 1 cut(s) 621
SchI GAGTC 2 cut(s) 71, 499
ScrFI CCNGG 4 cut(s) 70, 294, 390, 495
SduI GDGCHC 2 cut(s) 113, 232
SetI ASST 7 cut(s) 150, 201, 288, 438, 531, 635, 649
SfaNI GCATC 2 cut(s) 363, 436
Sfr274I CTCGAG 1 cut(s) 85
SinI GGWCC 1 cut(s) 621
SlaI CTCGAG 1 cut(s) 85
SmlI CTYRAG 2 cut(s) 85, 702
SmoI CTYRAG 2 cut(s) 85, 702
Sse9I AATT 3 cut(s) 254, 270, 422
SsiI CCGC 7 cut(s) 157, 260, 297, 300, 309, 449, 458
SspMI CTAG 3 cut(s) 54, 65, 630
StyD4I CCNGG 4 cut(s) 68, 292, 388, 493
TaaI ACNGT 5 cut(s) 115, 234, 322, 328, 503
TaiI ACGT 2 cut(s) 150, 201
TaqI TCGA 4 cut(s) 86, 144, 226, 275
TasI AATT 3 cut(s) 254, 270, 422
TauI GCSGC 3 cut(s) 159, 300, 303
Tru1I TTAA 1 cut(s) 739
Tru9I TTAA 1 cut(s) 739
TscAI CASTG 1 cut(s) 237
TseFI GTSAC 1 cut(s) 30
TseI GCWGC 1 cut(s) 403
Tsp45I GTSAC 1 cut(s) 30
TspDTI ATGAA 2 cut(s) 625, 690
TspGWI ACGGA 5 cut(s) 234, 293, 387, 537, 711
TspRI CASTG 1 cut(s) 237
VpaK11BI GGWCC 1 cut(s) 621
XapI RAATTY 2 cut(s) 270, 422
XhoI CTCGAG 1 cut(s) 85
XmnI GAANNNNTTC 1 cut(s) 13
XspI CTAG 3 cut(s) 54, 65, 630
ZraI GACGTC 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.