pycom06g03160

Heat shock factor protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0008651
Physical Location & Seq
Forward (+)
3983165 .. 3983895
731 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 330 bp
ATGGGGGGCGCTGATAACAACAACCGCGACGAGATATCAGTGGCCGCCGGCGAACAACAGGCGGCTTCGGGTTCAAGTTCGGCTCCGGCGCCAGCACCCTTACTGAAATCCAACGCCCCGCCGCCGTTCCTGTGTAAGACGTACGACATGGTGGACGACCCGGGTACCGACCCGGTCGTGTCGTGGACCCCCACCAACAATAGCTTTGTGGTTTGGAAACCGCCGGAGTTCGCCAGGGACCTCCTTCCCAAGTACTTCAAGCACAATAACTTCTCTAGCTTTGTTAGGCAGTTGAACACCTACCAATCAGATGGAGCAGAAGTGGGATAA

Protein Analysis

110

Amino Acids

11.75

Weight (kDa)

4.68

Isoelectric Point (pI)

40.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 43 - 102 9.4e-21 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 164
AccB1I GGYRCC 2 cut(s) 88, 164
AccII CGCG 1 cut(s) 27
AciI CCGC 6 cut(s) 25, 45, 62, 119, 122, 221
AcoI YGGCCR 1 cut(s) 42
AcyI GRCGYC 1 cut(s) 89
AfaI GTAC 3 cut(s) 143, 166, 254
AgsI TTSAA 3 cut(s) 75, 259, 295
AjnI CCWGG 1 cut(s) 233
AluBI AGCT 2 cut(s) 204, 279
AluI AGCT 2 cut(s) 204, 279
Ama87I CYCGRG 1 cut(s) 160
AoxI GGCC 1 cut(s) 42
Asp718I GGTACC 1 cut(s) 164
AspLEI GCGC 2 cut(s) 11, 91
AspS9I GGNCC 2 cut(s) 186, 238
AsuC2I CCSGG 3 cut(s) 161, 162, 173
AvaI CYCGRG 1 cut(s) 160
AvaII GGWCC 2 cut(s) 186, 238
BaeI ACNNNNGTAYC 2 cut(s) 148, 181
BanI GGYRCC 2 cut(s) 88, 164
BccI CCATC 1 cut(s) 305
BceAI ACGGC 1 cut(s) 109
BciT130I CCWGG 1 cut(s) 235
BcnI CCSGG 3 cut(s) 161, 162, 173
BfaI CTAG 1 cut(s) 276
BfoI RGCGCY 2 cut(s) 12, 92
BisI GCNGC 3 cut(s) 45, 63, 122
BlsI GCNGC 3 cut(s) 46, 64, 123
BmcAI AGTACT 1 cut(s) 254
Bme1390I CCNGG 4 cut(s) 161, 162, 173, 235
Bme18I GGWCC 2 cut(s) 186, 238
BmeT110I CYCGRG 1 cut(s) 160
BmgT120I GGNCC 2 cut(s) 186, 238
BmiI GGNNCC 5 cut(s) 84, 90, 166, 188, 239
BmrFI CCNGG 4 cut(s) 161, 162, 173, 235
BpuMI CCSGG 3 cut(s) 161, 162, 173
BsaHI GRCGYC 1 cut(s) 89
BsaJI CCNNGG 2 cut(s) 160, 234
BsaXI ACNNNNNCTCC 1 cut(s) 306
Bse118I RCCGGY 1 cut(s) 47
BseBI CCWGG 1 cut(s) 235
BseDI CCNNGG 2 cut(s) 160, 234
Bsh1236I CGCG 1 cut(s) 27
Bsh1285I CGRYCG 1 cut(s) 177
BshFI GGCC 1 cut(s) 44
BshNI GGYRCC 2 cut(s) 88, 164
BsiEI CGRYCG 1 cut(s) 177
BsiHKCI CYCGRG 1 cut(s) 160
BsiSI CCGG 5 cut(s) 48, 86, 161, 173, 224
BsiWI CGTACG 1 cut(s) 141
BslFI GGGAC 1 cut(s) 251
BsmFI GGGAC 1 cut(s) 251
BsnI GGCC 1 cut(s) 44
BsoBI CYCGRG 1 cut(s) 160
BspACI CCGC 6 cut(s) 25, 45, 62, 119, 122, 221
BspANI GGCC 1 cut(s) 44
BspFNI CGCG 1 cut(s) 27
BspLI GGNNCC 5 cut(s) 84, 90, 166, 188, 239
BspT107I GGYRCC 2 cut(s) 88, 164
BsrFI RCCGGY 1 cut(s) 47
BssAI RCCGGY 1 cut(s) 47
BssECI CCNNGG 2 cut(s) 160, 234
BssNI GRCGYC 1 cut(s) 89
Bst2UI CCWGG 1 cut(s) 235
BstACI GRCGYC 1 cut(s) 89
BstC8I GCNNGC 2 cut(s) 49, 93
BstFNI CGCG 1 cut(s) 27
BstH2I RGCGCY 2 cut(s) 12, 92
BstHHI GCGC 2 cut(s) 11, 91
BstMCI CGRYCG 1 cut(s) 177
BstNI CCWGG 1 cut(s) 235
BstSCI CCNGG 4 cut(s) 159, 160, 171, 233
BstUI CGCG 1 cut(s) 27
BstXI CCANNNNNNTGG 1 cut(s) 311
BsuRI GGCC 1 cut(s) 44
BtsIMutI CAGTG 1 cut(s) 45
Cac8I GCNNGC 2 cut(s) 49, 93
CfoI GCGC 2 cut(s) 11, 91
Cfr10I RCCGGY 1 cut(s) 47
Cfr13I GGNCC 2 cut(s) 186, 238
Cfr9I CCCGGG 1 cut(s) 160
Csp6I GTAC 3 cut(s) 142, 165, 253
CviAII CATG 1 cut(s) 148
CviJI RGCY 5 cut(s) 44, 65, 83, 204, 279
CviKI_1 RGCY 5 cut(s) 44, 65, 83, 204, 279
CviQI GTAC 3 cut(s) 142, 165, 253
DinI GGCGCC 1 cut(s) 90
EaeI YGGCCR 1 cut(s) 42
Eco32I GATATC 1 cut(s) 36
Eco47I GGWCC 2 cut(s) 186, 238
Eco88I CYCGRG 1 cut(s) 160
EcoO109I RGGNCCY 1 cut(s) 238
EcoRII CCWGG 1 cut(s) 233
EcoRV GATATC 1 cut(s) 36
EgeI GGCGCC 1 cut(s) 90
EheI GGCGCC 1 cut(s) 90
FaeI CATG 1 cut(s) 151
FaiI YATR 1 cut(s) 149
FaqI GGGAC 1 cut(s) 251
FatI CATG 1 cut(s) 147
FauI CCCGC 1 cut(s) 126
Fnu4HI GCNGC 3 cut(s) 45, 63, 122
Fsp4HI GCNGC 3 cut(s) 45, 63, 122
FspBI CTAG 1 cut(s) 276
GlaI GCGC 2 cut(s) 10, 90
GluI GCNGC 3 cut(s) 45, 63, 122
HaeII RGCGCY 2 cut(s) 12, 92
HaeIII GGCC 1 cut(s) 44
HapII CCGG 5 cut(s) 48, 86, 161, 173, 224
HhaI GCGC 2 cut(s) 11, 91
Hin1I GRCGYC 1 cut(s) 89
Hin1II CATG 1 cut(s) 151
Hin6I GCGC 2 cut(s) 9, 89
HinP1I GCGC 2 cut(s) 9, 89
HpaII CCGG 5 cut(s) 48, 86, 161, 173, 224
Hpy166II GTNNAC 2 cut(s) 154, 186
Hpy188I TCNGA 1 cut(s) 310
Hpy8I GTNNAC 2 cut(s) 154, 186
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 1 cut(s) 254
HpyCH4IV ACGT 1 cut(s) 140
HpySE526I ACGT 1 cut(s) 140
Hsp92I GRCGYC 1 cut(s) 89
Hsp92II CATG 1 cut(s) 151
HspAI GCGC 2 cut(s) 9, 89
KasI GGCGCC 1 cut(s) 88
KpnI GGTACC 1 cut(s) 168
KroI GCCGGC 1 cut(s) 47
KroNI GCCGGC 1 cut(s) 49
LmnI GCTCC 2 cut(s) 88, 314
MaeI CTAG 1 cut(s) 276
MaeII ACGT 1 cut(s) 140
Mly113I GGCGCC 1 cut(s) 89
MmeI TCCRAC 1 cut(s) 135
MnlI CCTC 1 cut(s) 251
MreI CGCCGGCG 1 cut(s) 47
MroNI GCCGGC 1 cut(s) 47
MspI CCGG 5 cut(s) 48, 86, 161, 173, 224
MspR9I CCNGG 4 cut(s) 161, 162, 173, 235
MvaI CCWGG 1 cut(s) 235
MvnI CGCG 1 cut(s) 27
NaeI GCCGGC 1 cut(s) 49
NarI GGCGCC 1 cut(s) 89
NciI CCSGG 3 cut(s) 161, 162, 173
NgoMIV GCCGGC 1 cut(s) 47
NlaIII CATG 1 cut(s) 151
NlaIV GGNNCC 5 cut(s) 84, 90, 166, 188, 239
PdiI GCCGGC 1 cut(s) 49
Pfl23II CGTACG 1 cut(s) 141
PflFI GACNNNGTC 1 cut(s) 173
PkrI GCNGC 3 cut(s) 46, 64, 123
PluTI GGCGCC 1 cut(s) 92
PpuMI RGGWCCY 1 cut(s) 238
Psp5II RGGWCCY 1 cut(s) 238
Psp6I CCWGG 1 cut(s) 233
PspGI CCWGG 1 cut(s) 233
PspLI CGTACG 1 cut(s) 141
PspN4I GGNNCC 5 cut(s) 84, 90, 166, 188, 239
PspPI GGNCC 2 cut(s) 186, 238
PspPPI RGGWCCY 1 cut(s) 238
PsyI GACNNNGTC 1 cut(s) 173
RsaI GTAC 3 cut(s) 143, 166, 254
RsaNI GTAC 3 cut(s) 142, 165, 253
SatI GCNGC 3 cut(s) 45, 63, 122
Sau96I GGNCC 2 cut(s) 186, 238
ScaI AGTACT 1 cut(s) 254
ScrFI CCNGG 4 cut(s) 161, 162, 173, 235
SetI ASST 5 cut(s) 143, 206, 243, 281, 302
SfoI GGCGCC 1 cut(s) 90
SgrAI CRCCGGYG 1 cut(s) 47
SinI GGWCC 2 cut(s) 186, 238
SmaI CCCGGG 1 cut(s) 162
SsiI CCGC 6 cut(s) 25, 45, 62, 119, 122, 221
SspDI GGCGCC 1 cut(s) 88
SspMI CTAG 1 cut(s) 276
StyD4I CCNGG 4 cut(s) 159, 160, 171, 233
TaiI ACGT 1 cut(s) 143
TatI WGTACW 1 cut(s) 252
TauI GCSGC 3 cut(s) 47, 65, 124
TscAI CASTG 1 cut(s) 45
TspMI CCCGGG 1 cut(s) 160
TspRI CASTG 1 cut(s) 45
Tth111I GACNNNGTC 1 cut(s) 173
VpaK11BI GGWCC 2 cut(s) 186, 238
XmaI CCCGGG 1 cut(s) 160
XspI CTAG 1 cut(s) 276
ZrmI AGTACT 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.