pycom06g06770

YT521-B-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0002140
Physical Location & Seq
Reverse (-)
10828889 .. 10831467
2579 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 768 bp
ATGGTTGTGTTGCAGAACTCTGGTAAAGTAATTTTAATATTCAGTGTCAACATGAGTGGTGCCTTCCAAGGGTATGCTCAAATGATGTCTTCTGTTGGATGGAGACGAGACAATGTATGGAGTCAAGGAAGTAGTAGAAGTAATCCATGGGGCCGCAGTTTTAAGGTCGAATGGCTGCGGCTAAATGATCTTCCTTTCCAAAAGACTCTTCATCTCAAGAATCCATTGAATGAGTACAAACCCGTTAAAATTAGCAGAGACTGCCAGGAGTTGTCTCCAGATGTTGGAGAAGCTCTTTGTGAGCTTCTTGATGGGACGGATGATGTGCACGGCTTACCGAACGGTATTCCCATGGATGATTTTCCTTCAAAAAGGCCTTGTATAGAGCCTCCATGTTCATTAGGGGAGGAAGAGTATAATGTGGGTCCCATGCATCTATTGTGGTCCCAGACACCCATGATGTATCCTTCGTTTCTTTACCAGCAGCAGGCTGAAGCAAATAGATATCATGTAGCAAATCAGAGATCTACTGAAAGTCGTCTAGCTAGCGCTCTTCTGACTGAAGATGATTTTTTGGAAATGTCTTATGAAGAATACCTGGAGGCCCAAAGCAGAAGTAGCAAGGAAGTATGTCTGCCTGGTTCTAGATCAGAGGAATCATCAAGAAGTAGAATGCAGGAACATGATTCAAACTCAAGCTATGGAAGCGACCGCAGCTGCTCAAGGAAAAGGCCTCATCATTCATCCCACAGGCAATCACAATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
YTH

Protein Analysis

256

Amino Acids

29.15

Weight (kDa)

5.78

Isoelectric Point (pI)

73.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YTH PF04146 5 - 103 7.2e-34 YT521-B-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 59
AccB7I CCANNNNNTGG 1 cut(s) 284
AciI CCGC 3 cut(s) 154, 178, 712
AcuI CTGAAG 2 cut(s) 513, 582
AfaI GTAC 1 cut(s) 236
AfeI AGCGCT 1 cut(s) 550
AfiI CCNNNNNNNGG 3 cut(s) 69, 284, 487
AgsI TTSAA 3 cut(s) 229, 369, 690
AjnI CCWGG 3 cut(s) 264, 597, 637
AjuI GAANNNNNNNTTGG 2 cut(s) 192, 224
AluBI AGCT 5 cut(s) 293, 304, 545, 699, 717
AluI AGCT 5 cut(s) 293, 304, 545, 699, 717
Alw21I GWGCWC 1 cut(s) 330
Alw26I GTCTC 4 cut(s) 97, 102, 252, 279
Alw44I GTGCAC 1 cut(s) 326
AlwNI CAGNNNCTG 1 cut(s) 261
Aor51HI AGCGCT 1 cut(s) 550
AoxI GGCC 4 cut(s) 151, 374, 603, 731
ApaLI GTGCAC 1 cut(s) 326
ApeKI GCWGC 4 cut(s) 175, 484, 714, 717
AspLEI GCGC 1 cut(s) 551
AspS9I GGNCC 4 cut(s) 151, 425, 444, 604
AsuNHI GCTAGC 1 cut(s) 545
AvaII GGWCC 2 cut(s) 425, 444
BaeGI GKGCMC 1 cut(s) 330
BanI GGYRCC 1 cut(s) 59
BbsI GAAGAC 1 cut(s) 81
Bbv12I GWGCWC 1 cut(s) 330
BbvI GCAGC 4 cut(s) 162, 496, 704, 726
BccI CCATC 2 cut(s) 93, 305
BceAI ACGGC 1 cut(s) 346
BciT130I CCWGG 3 cut(s) 266, 599, 639
BciVI GTATCC 1 cut(s) 474
BcoDI GTCTC 4 cut(s) 97, 102, 252, 279
BfaI CTAG 3 cut(s) 542, 546, 645
BfoI RGCGCY 1 cut(s) 552
BfuI GTATCC 1 cut(s) 474
BglII AGATCT 1 cut(s) 524
BisI GCNGC 6 cut(s) 154, 176, 179, 485, 715, 718
BlsI GCNGC 6 cut(s) 155, 177, 180, 486, 716, 719
Bme1390I CCNGG 3 cut(s) 266, 599, 639
Bme18I GGWCC 2 cut(s) 425, 444
BmgT120I GGNCC 4 cut(s) 151, 425, 444, 604
BmiI GGNNCC 5 cut(s) 61, 152, 426, 427, 446
BmrFI CCNGG 3 cut(s) 266, 599, 639
BmsI GCATC 1 cut(s) 442
BmtI GCTAGC 1 cut(s) 549
BpiI GAAGAC 1 cut(s) 81
BpmI CTGGAG 2 cut(s) 261, 620
BpuEI CTTGAG 3 cut(s) 200, 679, 706
BsaJI CCNNGG 3 cut(s) 67, 146, 351
BsaXI ACNNNNNCTCC 2 cut(s) 398, 428
Bsc4I CCNNNNNNNGG 3 cut(s) 69, 284, 487
BseBI CCWGG 3 cut(s) 266, 599, 639
BseDI CCNNGG 3 cut(s) 67, 146, 351
BseGI GGATG 4 cut(s) 104, 325, 361, 743
BseLI CCNNNNNNNGG 3 cut(s) 69, 284, 487
BseSI GKGCMC 1 cut(s) 330
BseXI GCAGC 4 cut(s) 162, 496, 704, 726
Bsh1285I CGRYCG 1 cut(s) 712
BshFI GGCC 4 cut(s) 153, 376, 605, 733
BshNI GGYRCC 1 cut(s) 59
BsiEI CGRYCG 1 cut(s) 712
BsiHKAI GWGCWC 1 cut(s) 330
BslFI GGGAC 3 cut(s) 328, 411, 430
BslI CCNNNNNNNGG 3 cut(s) 69, 284, 487
BsmAI GTCTC 4 cut(s) 97, 102, 252, 279
BsmBI CGTCTC 1 cut(s) 97
BsmFI GGGAC 3 cut(s) 328, 411, 430
BsmI GAATGC 1 cut(s) 678
BsnI GGCC 4 cut(s) 153, 376, 605, 733
Bsp1286I GDGCHC 1 cut(s) 330
Bsp143I GATC 3 cut(s) 187, 524, 647
Bsp19I CCATGG 2 cut(s) 146, 351
BspACI CCGC 3 cut(s) 154, 178, 712
BspANI GGCC 4 cut(s) 153, 376, 605, 733
BspLI GGNNCC 5 cut(s) 61, 152, 426, 427, 446
BspOI GCTAGC 1 cut(s) 549
BspQI GCTCTTC 1 cut(s) 558
BspT107I GGYRCC 1 cut(s) 59
BssECI CCNNGG 3 cut(s) 67, 146, 351
BssMI GATC 3 cut(s) 187, 524, 647
BssT1I CCWWGG 3 cut(s) 67, 146, 351
Bst2UI CCWGG 3 cut(s) 266, 599, 639
Bst4CI ACNGT 1 cut(s) 344
Bst6I CTCTTC 3 cut(s) 213, 405, 558
BstAPI GCANNNNNTGC 1 cut(s) 261
BstC8I GCNNGC 2 cut(s) 489, 547
BstDSI CCRYGG 2 cut(s) 146, 351
BstF5I GGATG 4 cut(s) 104, 325, 361, 743
BstH2I RGCGCY 1 cut(s) 552
BstHHI GCGC 1 cut(s) 551
BstKTI GATC 3 cut(s) 190, 527, 650
BstMAI GTCTC 4 cut(s) 97, 102, 252, 279
BstMBI GATC 3 cut(s) 187, 524, 647
BstMCI CGRYCG 1 cut(s) 712
BstMWI GCNNNNNNNGC 4 cut(s) 261, 618, 705, 714
BstNI CCWGG 3 cut(s) 266, 599, 639
BstSCI CCNGG 3 cut(s) 264, 597, 637
BstSLI GKGCMC 1 cut(s) 330
BstV1I GCAGC 4 cut(s) 162, 496, 704, 726
BstV2I GAAGAC 1 cut(s) 81
BstX2I RGATCY 1 cut(s) 524
BstYI RGATCY 1 cut(s) 524
BsuI GTATCC 1 cut(s) 474
BsuRI GGCC 4 cut(s) 153, 376, 605, 733
BtgI CCRYGG 2 cut(s) 146, 351
BtsCI GGATG 4 cut(s) 104, 325, 361, 743
BtsIMutI CAGTG 1 cut(s) 49
Cac8I GCNNGC 2 cut(s) 489, 547
CaiI CAGNNNCTG 1 cut(s) 261
CfoI GCGC 1 cut(s) 551
Cfr13I GGNCC 4 cut(s) 151, 425, 444, 604
Csp6I GTAC 1 cut(s) 235
CspCI CAANNNNNGTGG 2 cut(s) 37, 72
CviAII CATG 8 cut(s) 52, 147, 352, 393, 430, 457, 509, 683
CviQI GTAC 1 cut(s) 235
DpnI GATC 3 cut(s) 189, 526, 649
DpnII GATC 3 cut(s) 187, 524, 647
Eam1104I CTCTTC 3 cut(s) 213, 405, 558
EarI CTCTTC 3 cut(s) 213, 405, 558
Eco130I CCWWGG 3 cut(s) 67, 146, 351
Eco147I AGGCCT 2 cut(s) 376, 733
Eco32I GATATC 1 cut(s) 506
Eco47I GGWCC 2 cut(s) 425, 444
Eco47III AGCGCT 1 cut(s) 550
Eco57I CTGAAG 2 cut(s) 513, 582
EcoO109I RGGNCCY 1 cut(s) 425
EcoRII CCWGG 3 cut(s) 264, 597, 637
EcoRV GATATC 1 cut(s) 506
EcoT14I CCWWGG 3 cut(s) 67, 146, 351
EcoT22I ATGCAT 1 cut(s) 435
ErhI CCWWGG 3 cut(s) 67, 146, 351
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 8 cut(s) 55, 150, 355, 396, 433, 460, 512, 686
FaqI GGGAC 3 cut(s) 328, 411, 430
FatI CATG 8 cut(s) 51, 146, 351, 392, 429, 456, 508, 682
Fnu4HI GCNGC 6 cut(s) 154, 176, 179, 485, 715, 718
FokI GGATG 4 cut(s) 111, 332, 368, 730
Fsp4HI GCNGC 6 cut(s) 154, 176, 179, 485, 715, 718
FspBI CTAG 3 cut(s) 542, 546, 645
GlaI GCGC 1 cut(s) 550
GluI GCNGC 6 cut(s) 154, 176, 179, 485, 715, 718
GsuI CTGGAG 2 cut(s) 261, 620
HaeII RGCGCY 1 cut(s) 552
HaeIII GGCC 4 cut(s) 153, 376, 605, 733
HhaI GCGC 1 cut(s) 551
Hin1II CATG 8 cut(s) 55, 150, 355, 396, 433, 460, 512, 686
Hin6I GCGC 1 cut(s) 549
HinP1I GCGC 1 cut(s) 549
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HinfI GANTC 5 cut(s) 121, 205, 220, 656, 686
Hpy166II GTNNAC 2 cut(s) 49, 328
Hpy188I TCNGA 3 cut(s) 522, 558, 652
Hpy188III TCNNGA 5 cut(s) 217, 278, 308, 645, 663
Hpy8I GTNNAC 2 cut(s) 49, 328
HpyAV CCTTC 3 cut(s) 73, 375, 477
HpyCH4III ACNGT 1 cut(s) 344
HpyCH4V TGCA 4 cut(s) 13, 328, 433, 676
HpyF10VI GCNNNNNNNGC 4 cut(s) 261, 618, 705, 714
Hsp92II CATG 8 cut(s) 55, 150, 355, 396, 433, 460, 512, 686
HspAI GCGC 1 cut(s) 549
KflI GGGWCCC 1 cut(s) 425
Kzo9I GATC 3 cut(s) 187, 524, 647
LguI GCTCTTC 1 cut(s) 558
Lsp1109I GCAGC 4 cut(s) 162, 496, 704, 726
LweI GCATC 1 cut(s) 442
MaeI CTAG 3 cut(s) 542, 546, 645
MalI GATC 3 cut(s) 189, 526, 649
MboI GATC 3 cut(s) 187, 524, 647
MboII GAAGA 7 cut(s) 81, 182, 200, 422, 545, 575, 602
MflI RGATCY 1 cut(s) 524
MhlI GDGCHC 1 cut(s) 330
MluCI AATT 2 cut(s) 30, 249
MlyI GAGTC 2 cut(s) 130, 199
MmeI TCCRAC 2 cut(s) 76, 265
MnlI CCTC 5 cut(s) 399, 400, 595, 646, 744
Mph1103I ATGCAT 1 cut(s) 435
MseI TTAA 3 cut(s) 35, 162, 246
MspA1I CMGCKG 1 cut(s) 717
MspR9I CCNGG 3 cut(s) 266, 599, 639
Mva1269I GAATGC 1 cut(s) 678
MvaI CCWGG 3 cut(s) 266, 599, 639
MwoI GCNNNNNNNGC 4 cut(s) 261, 618, 705, 714
NcoI CCATGG 2 cut(s) 146, 351
NdeII GATC 3 cut(s) 187, 524, 647
NheI GCTAGC 1 cut(s) 545
NlaIII CATG 8 cut(s) 55, 150, 355, 396, 433, 460, 512, 686
NlaIV GGNNCC 5 cut(s) 61, 152, 426, 427, 446
NsiI ATGCAT 1 cut(s) 435
PceI AGGCCT 2 cut(s) 376, 733
PciSI GCTCTTC 1 cut(s) 558
PctI GAATGC 1 cut(s) 678
PfeI GAWTC 3 cut(s) 220, 656, 686
PflMI CCANNNNNTGG 1 cut(s) 284
PkrI GCNGC 6 cut(s) 155, 177, 180, 486, 716, 719
PleI GAGTC 2 cut(s) 129, 199
PpsI GAGTC 2 cut(s) 129, 199
PpuMI RGGWCCY 1 cut(s) 425
Psp5II RGGWCCY 1 cut(s) 425
Psp6I CCWGG 3 cut(s) 264, 597, 637
PspGI CCWGG 3 cut(s) 264, 597, 637
PspN4I GGNNCC 5 cut(s) 61, 152, 426, 427, 446
PspPI GGNCC 4 cut(s) 151, 425, 444, 604
PspPPI RGGWCCY 1 cut(s) 425
PstNI CAGNNNCTG 1 cut(s) 261
PsuI RGATCY 1 cut(s) 524
PvuII CAGCTG 1 cut(s) 717
RsaI GTAC 1 cut(s) 236
RsaNI GTAC 1 cut(s) 235
SapI GCTCTTC 1 cut(s) 558
SaqAI TTAA 3 cut(s) 35, 162, 246
SatI GCNGC 6 cut(s) 154, 176, 179, 485, 715, 718
Sau3AI GATC 3 cut(s) 187, 524, 647
Sau96I GGNCC 4 cut(s) 151, 425, 444, 604
SchI GAGTC 2 cut(s) 130, 199
ScrFI CCNGG 3 cut(s) 266, 599, 639
SduI GDGCHC 1 cut(s) 330
SetI ASST 7 cut(s) 168, 295, 306, 547, 600, 701, 719
SfaNI GCATC 1 cut(s) 442
SinI GGWCC 2 cut(s) 425, 444
SmlI CTYRAG 3 cut(s) 215, 694, 721
SmoI CTYRAG 3 cut(s) 215, 694, 721
Sse9I AATT 2 cut(s) 30, 249
SseBI AGGCCT 2 cut(s) 376, 733
SsiI CCGC 3 cut(s) 154, 178, 712
SspI AATATT 1 cut(s) 39
SspMI CTAG 3 cut(s) 542, 546, 645
StuI AGGCCT 2 cut(s) 376, 733
StyD4I CCNGG 3 cut(s) 264, 597, 637
StyI CCWWGG 3 cut(s) 67, 146, 351
TaaI ACNGT 1 cut(s) 344
TaqI TCGA 1 cut(s) 168
TasI AATT 2 cut(s) 30, 249
TatI WGTACW 1 cut(s) 234
TauI GCSGC 2 cut(s) 156, 181
TfiI GAWTC 3 cut(s) 220, 656, 686
Tru1I TTAA 3 cut(s) 35, 162, 246
Tru9I TTAA 3 cut(s) 35, 162, 246
TscAI CASTG 1 cut(s) 49
TseI GCWGC 4 cut(s) 175, 484, 714, 717
TspDTI ATGAA 4 cut(s) 200, 387, 603, 732
TspGWI ACGGA 1 cut(s) 332
TspRI CASTG 1 cut(s) 49
Van91I CCANNNNNTGG 1 cut(s) 284
VneI GTGCAC 1 cut(s) 326
VpaK11BI GGWCC 2 cut(s) 425, 444
XbaI TCTAGA 1 cut(s) 644
XspI CTAG 3 cut(s) 542, 546, 645
Zsp2I ATGCAT 1 cut(s) 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.