pycom06g07980

Transcription factor S-II (TFIIS), central domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0005626
Physical Location & Seq
Forward (+)
13033504 .. 13036796
3293 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 849 bp
ATGGCACTAACTGGAGACACACTACGTGACAGATGGTTGGAGAAACTTCTACAAAATATCCGGCACCACATATGGAACTCTGCTGACACTACAAACGGTATTGAAAAGAGAAAATCTGGCTTTGATGATATTGATCATGAAAGTGACCGTAAGCCCACAGAAGTTGGAAATGGATCTCAGGATAATGTCAAGAGTGGCAACACGTCTTTTCTCTGGCCAGAGGATGCTGTTCGTGCAGTTACTTCTCTAGAGAAGGCTTCGCATGAGTCTCTCTCCTCAGATTTCCTAAAGTATAACCAGAAGTTGCGGCAACTGGGATTCAATCTCCAGAAAAATTCATTCCTAGCCCGTCGTCTGCTAAATCGAGAGTTGGAACCTTTAACGATAGTGAACATGTCACCTGAGGAGTTAAAGGAGGGTTTAACAGCTGAGGAGAGAGCAAAGAAGGAGCCTGATGATTGGGAGCGTATGCAGATGACTGACGCTCGTTGCTCAAGATGCTCAGAGTTCAAAGTGGGAGTTAGGGACATTATTCAAGCAGGACACAGTGCTCGCTATCAGCTGGAGTGTATTGCCTGTGGTCATTCATGGTATGCTGCCCGGGATGCGGTTTCAATGCTCACAATTGATGCACCCAGTTCTAACAAGAATGTGGGGACGGCTCCTTTGGCCACTGCCAAGTTTGAAGATGTTGAAAAGAAGCTGGCGAGCCCACGTGAGCCTGACAAGACAACCAAGGACGCTCTAAGAAAAACAACTGAAGCATATATGCCGGTGTTGGAAGCGCAAAGATCACTTAGCAAGTTCAAAAGGGAAGAAAATTCTGATTCTGCAAAGAAAGCCGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BAH DUF3527 TFIIS_M

Protein Analysis

283

Amino Acids

31.95

Weight (kDa)

6.63

Isoelectric Point (pI)

49.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIS_M PF07500 75 - 153 3.6e-14 Transcription factor S-II (TFIIS), central domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 63
AciI CCGC 2 cut(s) 307, 608
AclWI GGATC 1 cut(s) 181
AcoI YGGCCR 2 cut(s) 215, 669
AcsI RAATTY 2 cut(s) 334, 820
AcuI CTGAAG 1 cut(s) 780
AcvI CACGTG 1 cut(s) 716
AdeI CACNNNGTG 1 cut(s) 26
AfiI CCNNNNNNNGG 1 cut(s) 607
AflIII ACRYGT 2 cut(s) 201, 393
AgsI TTSAA 8 cut(s) 104, 322, 511, 536, 615, 686, 695, 808
AjiI CACGTC 1 cut(s) 204
AluBI AGCT 3 cut(s) 428, 562, 703
AluI AGCT 3 cut(s) 428, 562, 703
Alw21I GWGCWC 1 cut(s) 553
Alw26I GTCTC 2 cut(s) 9, 273
AlwI GGATC 1 cut(s) 181
Ama87I CYCGRG 1 cut(s) 600
AoxI GGCC 2 cut(s) 215, 669
ApeKI GCWGC 1 cut(s) 596
ApoI RAATTY 2 cut(s) 334, 820
ArsI GACNNNNNNTTYG 2 cut(s) 649, 681
AspLEI GCGC 1 cut(s) 787
AsuC2I CCSGG 2 cut(s) 601, 602
AsuHPI GGTGA 1 cut(s) 390
AvaI CYCGRG 1 cut(s) 600
AxyI CCTNAGG 1 cut(s) 402
BalI TGGCCA 2 cut(s) 217, 671
BanI GGYRCC 1 cut(s) 63
BanII GRGCYC 1 cut(s) 713
BbrPI CACGTG 1 cut(s) 716
Bbv12I GWGCWC 1 cut(s) 553
BbvCI CCTCAGC 1 cut(s) 429
BbvI GCAGC 1 cut(s) 583
BccI CCATC 1 cut(s) 27
BceAI ACGGC 1 cut(s) 675
BclI TGATCA 1 cut(s) 133
BcnI CCSGG 2 cut(s) 601, 602
BcoDI GTCTC 2 cut(s) 9, 273
BfaI CTAG 2 cut(s) 248, 344
BisI GCNGC 2 cut(s) 308, 597
BlsI GCNGC 2 cut(s) 309, 598
Bme1390I CCNGG 2 cut(s) 601, 602
BmeT110I CYCGRG 1 cut(s) 600
BmgBI CACGTC 1 cut(s) 204
BmiI GGNNCC 4 cut(s) 65, 375, 450, 663
BmrFI CCNGG 2 cut(s) 601, 602
BmrI ACTGGG 2 cut(s) 323, 630
BmsI GCATC 4 cut(s) 214, 488, 595, 619
BmuI ACTGGG 2 cut(s) 323, 630
BplI GAGNNNNNCTC 2 cut(s) 257, 289
BpmI CTGGAG 3 cut(s) 33, 311, 584
Bpu10I CCTNAGC 1 cut(s) 429
BpuEI CTTGAG 1 cut(s) 478
BpuMI CCSGG 2 cut(s) 601, 602
BsaAI YACGTR 2 cut(s) 26, 716
BsaBI GATNNNNATC 1 cut(s) 132
BsaJI CCNNGG 2 cut(s) 600, 735
Bsc4I CCNNNNNNNGG 1 cut(s) 607
Bse118I RCCGGY 1 cut(s) 772
Bse1I ACTGG 3 cut(s) 16, 318, 636
Bse21I CCTNAGG 1 cut(s) 402
Bse8I GATNNNNATC 1 cut(s) 132
BseDI CCNNGG 2 cut(s) 600, 735
BseGI GGATG 2 cut(s) 229, 610
BseJI GATNNNNATC 1 cut(s) 132
BseLI CCNNNNNNNGG 1 cut(s) 607
BseMII CTCAG 5 cut(s) 191, 291, 393, 420, 516
BseNI ACTGG 3 cut(s) 16, 318, 636
BseRI GAGGAG 3 cut(s) 265, 419, 446
BseXI GCAGC 1 cut(s) 583
BsgI GTGCAG 1 cut(s) 255
BshFI GGCC 2 cut(s) 217, 671
BshNI GGYRCC 1 cut(s) 63
BsiHKAI GWGCWC 1 cut(s) 553
BsiHKCI CYCGRG 1 cut(s) 600
BsiSI CCGG 3 cut(s) 61, 601, 773
BslFI GGGAC 2 cut(s) 539, 670
BslI CCNNNNNNNGG 1 cut(s) 607
BsmAI GTCTC 2 cut(s) 9, 273
BsmFI GGGAC 2 cut(s) 539, 670
BsnI GGCC 2 cut(s) 217, 671
BsoBI CYCGRG 1 cut(s) 600
Bsp1286I GDGCHC 2 cut(s) 553, 713
Bsp143I GATC 3 cut(s) 133, 173, 791
BspACI CCGC 2 cut(s) 307, 608
BspANI GGCC 2 cut(s) 217, 671
BspCNI CTCAG 5 cut(s) 190, 290, 394, 421, 515
BspHI TCATGA 1 cut(s) 136
BspLI GGNNCC 4 cut(s) 65, 375, 450, 663
BspPI GGATC 1 cut(s) 181
BspT107I GGYRCC 1 cut(s) 63
BsrFI RCCGGY 1 cut(s) 772
BsrI ACTGG 3 cut(s) 16, 318, 636
BssAI RCCGGY 1 cut(s) 772
BssECI CCNNGG 2 cut(s) 600, 735
BssMI GATC 3 cut(s) 133, 173, 791
BssT1I CCWWGG 1 cut(s) 735
Bst4CI ACNGT 3 cut(s) 98, 149, 548
BstBAI YACGTR 2 cut(s) 26, 716
BstC8I GCNNGC 3 cut(s) 553, 705, 709
BstDEI CTNAG 7 cut(s) 177, 277, 402, 429, 502, 746, 797
BstF5I GGATG 2 cut(s) 229, 610
BstHHI GCGC 1 cut(s) 787
BstKTI GATC 3 cut(s) 136, 176, 794
BstMAI GTCTC 2 cut(s) 9, 273
BstMBI GATC 3 cut(s) 133, 173, 791
BstMWI GCNNNNNNNGC 5 cut(s) 233, 498, 605, 668, 839
BstNSI RCATGY 1 cut(s) 397
BstSCI CCNGG 2 cut(s) 599, 600
BstV1I GCAGC 1 cut(s) 583
BstX2I RGATCY 1 cut(s) 173
BstYI RGATCY 1 cut(s) 173
Bsu36I CCTNAGG 1 cut(s) 402
BsuRI GGCC 2 cut(s) 217, 671
BtrI CACGTC 1 cut(s) 204
BtsCI GGATG 2 cut(s) 229, 610
BtsI GCAGTG 1 cut(s) 672
BtsIMutI CAGTG 2 cut(s) 553, 672
Cac8I GCNNGC 3 cut(s) 553, 705, 709
CciI TCATGA 1 cut(s) 136
CfoI GCGC 1 cut(s) 787
Cfr10I RCCGGY 1 cut(s) 772
Cfr9I CCCGGG 1 cut(s) 600
CseI GACGC 2 cut(s) 491, 749
CspCI CAANNNNNGTGG 2 cut(s) 145, 180
CviAII CATG 4 cut(s) 137, 263, 394, 588
DdeI CTNAG 7 cut(s) 177, 277, 402, 429, 502, 746, 797
DpnI GATC 3 cut(s) 135, 175, 793
DpnII GATC 3 cut(s) 133, 173, 791
DraIII CACNNNGTG 1 cut(s) 26
EaeI YGGCCR 2 cut(s) 215, 669
Eco130I CCWWGG 1 cut(s) 735
Eco24I GRGCYC 1 cut(s) 713
Eco57I CTGAAG 1 cut(s) 780
Eco72I CACGTG 1 cut(s) 716
Eco81I CCTNAGG 1 cut(s) 402
Eco88I CYCGRG 1 cut(s) 600
EcoT14I CCWWGG 1 cut(s) 735
EcoT38I GRGCYC 1 cut(s) 713
ErhI CCWWGG 1 cut(s) 735
FaeI CATG 4 cut(s) 140, 266, 397, 591
FaqI GGGAC 2 cut(s) 539, 670
FatI CATG 4 cut(s) 136, 262, 393, 587
FauNDI CATATG 1 cut(s) 71
FbaI TGATCA 1 cut(s) 133
Fnu4HI GCNGC 2 cut(s) 308, 597
FokI GGATG 2 cut(s) 236, 617
FriOI GRGCYC 1 cut(s) 713
Fsp4HI GCNGC 2 cut(s) 308, 597
FspBI CTAG 2 cut(s) 248, 344
GlaI GCGC 1 cut(s) 786
GluI GCNGC 2 cut(s) 308, 597
GsuI CTGGAG 3 cut(s) 33, 311, 584
HaeIII GGCC 2 cut(s) 217, 671
HapII CCGG 3 cut(s) 61, 601, 773
HgaI GACGC 2 cut(s) 491, 749
HhaI GCGC 1 cut(s) 787
Hin1II CATG 4 cut(s) 140, 266, 397, 591
Hin6I GCGC 1 cut(s) 785
HinP1I GCGC 1 cut(s) 785
HinfI GANTC 3 cut(s) 266, 318, 827
HpaII CCGG 3 cut(s) 61, 601, 773
HphI GGTGA 1 cut(s) 390
Hpy166II GTNNAC 1 cut(s) 391
Hpy188I TCNGA 3 cut(s) 280, 505, 826
Hpy188III TCNNGA 7 cut(s) 137, 179, 190, 248, 328, 365, 495
Hpy8I GTNNAC 1 cut(s) 391
Hpy99I CGWCG 1 cut(s) 354
HpyAV CCTTC 2 cut(s) 247, 439
HpyCH4III ACNGT 3 cut(s) 98, 149, 548
HpyCH4IV ACGT 3 cut(s) 25, 203, 715
HpyCH4V TGCA 4 cut(s) 236, 472, 632, 833
HpyF10VI GCNNNNNNNGC 5 cut(s) 233, 498, 605, 668, 839
HpyF3I CTNAG 7 cut(s) 177, 277, 402, 429, 502, 746, 797
HpySE526I ACGT 3 cut(s) 25, 203, 715
Hsp92II CATG 4 cut(s) 140, 266, 397, 591
HspAI GCGC 1 cut(s) 785
Ksp22I TGATCA 1 cut(s) 133
Kzo9I GATC 3 cut(s) 133, 173, 791
LmnI GCTCC 3 cut(s) 448, 463, 667
Lsp1109I GCAGC 1 cut(s) 583
LweI GCATC 4 cut(s) 214, 488, 595, 619
MaeI CTAG 2 cut(s) 248, 344
MaeII ACGT 3 cut(s) 25, 203, 715
MaeIII GTNAC 4 cut(s) 26, 143, 238, 396
MalI GATC 3 cut(s) 135, 175, 793
MboI GATC 3 cut(s) 133, 173, 791
MboII GAAGA 2 cut(s) 698, 827
MfeI CAATTG 1 cut(s) 624
MflI RGATCY 1 cut(s) 173
MhlI GDGCHC 2 cut(s) 553, 713
MlsI TGGCCA 2 cut(s) 217, 671
MluCI AATT 3 cut(s) 334, 624, 820
MluNI TGGCCA 2 cut(s) 217, 671
MlyI GAGTC 1 cut(s) 275
MmeI TCCRAC 4 cut(s) 18, 145, 351, 759
MnlI CCTC 5 cut(s) 214, 286, 397, 409, 424
Mox20I TGGCCA 2 cut(s) 217, 671
MscI TGGCCA 2 cut(s) 217, 671
MseI TTAA 3 cut(s) 380, 410, 422
MslI CAYNNNNRTG 1 cut(s) 141
Msp20I TGGCCA 2 cut(s) 217, 671
MspA1I CMGCKG 2 cut(s) 428, 562
MspI CCGG 3 cut(s) 61, 601, 773
MspR9I CCNGG 2 cut(s) 601, 602
MunI CAATTG 1 cut(s) 624
MwoI GCNNNNNNNGC 5 cut(s) 233, 498, 605, 668, 839
NciI CCSGG 2 cut(s) 601, 602
NdeI CATATG 1 cut(s) 71
NdeII GATC 3 cut(s) 133, 173, 791
NlaIII CATG 4 cut(s) 140, 266, 397, 591
NlaIV GGNNCC 4 cut(s) 65, 375, 450, 663
NmuCI GTSAC 3 cut(s) 26, 143, 396
NspI RCATGY 1 cut(s) 397
PagI TCATGA 1 cut(s) 136
PciI ACATGT 1 cut(s) 393
PfeI GAWTC 2 cut(s) 318, 827
PkrI GCNGC 2 cut(s) 309, 598
PleI GAGTC 1 cut(s) 274
PmaCI CACGTG 1 cut(s) 716
PmlI CACGTG 1 cut(s) 716
PpsI GAGTC 1 cut(s) 274
Ppu21I YACGTR 2 cut(s) 26, 716
PscI ACATGT 1 cut(s) 393
PspCI CACGTG 1 cut(s) 716
PspN4I GGNNCC 4 cut(s) 65, 375, 450, 663
PsuI RGATCY 1 cut(s) 173
PvuII CAGCTG 2 cut(s) 428, 562
RseI CAYNNNNRTG 1 cut(s) 141
SaqAI TTAA 3 cut(s) 380, 410, 422
SatI GCNGC 2 cut(s) 308, 597
Sau3AI GATC 3 cut(s) 133, 173, 791
SchI GAGTC 1 cut(s) 275
ScrFI CCNGG 2 cut(s) 601, 602
SduI GDGCHC 2 cut(s) 553, 713
SetI ASST 8 cut(s) 28, 206, 379, 403, 430, 564, 705, 718
SfaNI GCATC 4 cut(s) 214, 488, 595, 619
SmaI CCCGGG 1 cut(s) 602
SmiMI CAYNNNNRTG 1 cut(s) 141
SmlI CTYRAG 1 cut(s) 493
SmoI CTYRAG 1 cut(s) 493
Sse9I AATT 3 cut(s) 334, 624, 820
SsiI CCGC 2 cut(s) 307, 608
SspMI CTAG 2 cut(s) 248, 344
StyD4I CCNGG 2 cut(s) 599, 600
StyI CCWWGG 1 cut(s) 735
TaaI ACNGT 3 cut(s) 98, 149, 548
TaiI ACGT 3 cut(s) 28, 206, 718
TaqI TCGA 1 cut(s) 364
TasI AATT 3 cut(s) 334, 624, 820
TauI GCSGC 1 cut(s) 310
TfiI GAWTC 2 cut(s) 318, 827
Tru1I TTAA 3 cut(s) 380, 410, 422
Tru9I TTAA 3 cut(s) 380, 410, 422
TscAI CASTG 2 cut(s) 553, 679
TseFI GTSAC 3 cut(s) 26, 143, 396
TseI GCWGC 1 cut(s) 596
Tsp45I GTSAC 3 cut(s) 26, 143, 396
TspDTI ATGAA 3 cut(s) 153, 327, 576
TspMI CCCGGG 1 cut(s) 600
TspRI CASTG 2 cut(s) 553, 679
XapI RAATTY 2 cut(s) 334, 820
XbaI TCTAGA 1 cut(s) 247
XceI RCATGY 1 cut(s) 397
XmaI CCCGGG 1 cut(s) 600
XspI CTAG 2 cut(s) 248, 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.