pycom06g09490

V-type proton ATPase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0007700
Physical Location & Seq
Reverse (-)
14810809 .. 14812846
2038 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 693 bp
ATGAACGACGCAGATGTCTCCAAGCAGATCCAGCAGATGGTGAGGTTCATCCGCCAAGAAGCTGAGGAGAAGGCCAACGAGATCTCCGTATCTGCTGAGGAAGAATTCAACATTGAGAAGTTGCAGCTCGTCGAGGCGGAGAAGAAGAAGATCAGGCAAGAATATGAGAAGAAGGAAAAGCAAGTCGACGTCCGAAAGAAGATTGAGTATTCCATGCAGCTCAATGCTTCTCGTATTAAAGTTCTTCAGGCCCAAGATGATGTGGTTAACTCCATGAAGGAGGCAGCATCCAAGGAGCTTCTGAATGTGAGCCGAGATCACCATGTGTACAAAAAGCTTCTGAAAGATCTTATCGTTCAGAATTTGCTTAGGCTGAAAGAGCCTGCTGTTTTGTTGCGTTGCCGGAAAGATGATCTACATCTGGTGGAGTCTGTTCTGGAATCAGCAGGACGGGAATATGCGGACAAAGCAAAGGTTCATTCACCTGAGATTATAGTGGACACTACTGTCTTTCTTCCACCTGCTCCTACCCATCATAATCCCCATGTTTCTTCCTGTTCTGGAGGTGTGGTCCTGGCTTCTCGGGATGGGAAGATTGTGTGTGAGAACACCCTTGATGCACGATTGGATGTTGTATTCCGTAAAAAACTTCCAGAGATCCGCAGAAAGCTCTTCGGTCAGGCTGCTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04145 ko04150 ko04721 ko04966 ko05110 ko05120 ko05323 map00190 map01100 map04145 map04150 map04721 map04966 map05110 map05120 map05323
Pfam Domains
Protein Families
vATP-synt_E

Protein Analysis

231

Amino Acids

26.26

Weight (kDa)

8.62

Isoelectric Point (pI)

44.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
vATP-synt_E PF01991 16 - 225 2.5e-71 ATP synthase (E/31 kDa) subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 529
AasI GACNNNNNNGTC 2 cut(s) 14, 506
AatII GACGTC 1 cut(s) 192
Acc36I ACCTGC 1 cut(s) 529
AccB7I CCANNNNNTGG 1 cut(s) 37
AccI GTMKAC 1 cut(s) 186
AciI CCGC 4 cut(s) 52, 137, 461, 661
AclWI GGATC 2 cut(s) 22, 652
AcsI RAATTY 2 cut(s) 104, 361
AcuI CTGAAG 1 cut(s) 230
AcyI GRCGYC 1 cut(s) 189
AdeI CACNNNGTG 1 cut(s) 325
AfaI GTAC 1 cut(s) 329
AfiI CCNNNNNNNGG 1 cut(s) 37
AgsI TTSAA 1 cut(s) 109
AjnI CCWGG 1 cut(s) 573
AluBI AGCT 6 cut(s) 62, 127, 220, 298, 337, 670
AluI AGCT 6 cut(s) 62, 127, 220, 298, 337, 670
Alw26I GTCTC 1 cut(s) 22
AlwI GGATC 2 cut(s) 22, 652
Ama87I CYCGRG 1 cut(s) 582
AoxI GGCC 2 cut(s) 72, 249
ApeKI GCWGC 5 cut(s) 124, 217, 284, 683, 686
ApoI RAATTY 2 cut(s) 104, 361
AspS9I GGNCC 2 cut(s) 250, 571
AsuHPI GGTGA 3 cut(s) 52, 311, 474
AvaI CYCGRG 1 cut(s) 582
AvaII GGWCC 1 cut(s) 571
BbvCI CCTCAGC 2 cut(s) 63, 96
BbvI GCAGC 5 cut(s) 136, 229, 296, 670, 673
BccI CCATC 3 cut(s) 31, 540, 581
BciT130I CCWGG 1 cut(s) 575
BcoDI GTCTC 1 cut(s) 22
BfuAI ACCTGC 1 cut(s) 529
BglII AGATCT 2 cut(s) 81, 346
BisI GCNGC 5 cut(s) 125, 218, 285, 684, 687
BlsI GCNGC 5 cut(s) 126, 219, 286, 685, 688
Bme1390I CCNGG 1 cut(s) 575
Bme18I GGWCC 1 cut(s) 571
BmeT110I CYCGRG 1 cut(s) 582
BmgT120I GGNCC 2 cut(s) 250, 571
BmrFI CCNGG 1 cut(s) 575
BmsI GCATC 2 cut(s) 296, 607
BpmI CTGGAG 1 cut(s) 582
Bpu10I CCTNAGC 3 cut(s) 63, 96, 368
BsaBI GATNNNNATC 1 cut(s) 417
BsaHI GRCGYC 1 cut(s) 189
BsaJI CCNNGG 1 cut(s) 291
BsaXI ACNNNNNCTCC 4 cut(s) 68, 98, 555, 585
Bsc4I CCNNNNNNNGG 1 cut(s) 37
Bse8I GATNNNNATC 1 cut(s) 417
BseBI CCWGG 1 cut(s) 575
BseDI CCNNGG 1 cut(s) 291
BseGI GGATG 4 cut(s) 48, 287, 592, 634
BseJI GATNNNNATC 1 cut(s) 417
BseLI CCNNNNNNNGG 1 cut(s) 37
BseMII CTCAG 3 cut(s) 54, 87, 477
BseRI GAGGAG 1 cut(s) 80
BseXI GCAGC 5 cut(s) 136, 229, 296, 670, 673
BshFI GGCC 2 cut(s) 74, 251
BsiHKCI CYCGRG 1 cut(s) 582
BsiSI CCGG 1 cut(s) 403
BslI CCNNNNNNNGG 1 cut(s) 37
BsmAI GTCTC 1 cut(s) 22
BsnI GGCC 2 cut(s) 74, 251
BsoBI CYCGRG 1 cut(s) 582
Bsp1407I TGTACA 1 cut(s) 327
Bsp143I GATC 7 cut(s) 27, 81, 150, 316, 346, 412, 657
BspACI CCGC 4 cut(s) 52, 137, 461, 661
BspANI GGCC 2 cut(s) 74, 251
BspCNI CTCAG 3 cut(s) 55, 88, 478
BspMI ACCTGC 1 cut(s) 529
BspPI GGATC 2 cut(s) 22, 652
BspQI GCTCTTC 1 cut(s) 677
BsrGI TGTACA 1 cut(s) 327
BssECI CCNNGG 1 cut(s) 291
BssMI GATC 7 cut(s) 27, 81, 150, 316, 346, 412, 657
BssNI GRCGYC 1 cut(s) 189
BssT1I CCWWGG 1 cut(s) 291
Bst2UI CCWGG 1 cut(s) 575
Bst4CI ACNGT 1 cut(s) 508
Bst6I CTCTTC 1 cut(s) 677
BstACI GRCGYC 1 cut(s) 189
BstAUI TGTACA 1 cut(s) 327
BstC8I GCNNGC 1 cut(s) 384
BstDEI CTNAG 4 cut(s) 63, 96, 368, 486
BstF5I GGATG 4 cut(s) 48, 287, 592, 634
BstKTI GATC 7 cut(s) 30, 84, 153, 319, 349, 415, 660
BstMAI GTCTC 1 cut(s) 22
BstMBI GATC 7 cut(s) 27, 81, 150, 316, 346, 412, 657
BstMWI GCNNNNNNNGC 3 cut(s) 31, 379, 467
BstNI CCWGG 1 cut(s) 575
BstSCI CCNGG 1 cut(s) 573
BstV1I GCAGC 5 cut(s) 136, 229, 296, 670, 673
BstX2I RGATCY 4 cut(s) 27, 81, 346, 657
BstYI RGATCY 4 cut(s) 27, 81, 346, 657
BsuRI GGCC 2 cut(s) 74, 251
BtsCI GGATG 4 cut(s) 48, 287, 592, 634
BveI ACCTGC 1 cut(s) 529
Cac8I GCNNGC 1 cut(s) 384
Cfr13I GGNCC 2 cut(s) 250, 571
CseI GACGC 1 cut(s) 17
Csp6I GTAC 1 cut(s) 328
CviAII CATG 4 cut(s) 214, 274, 323, 545
CviQI GTAC 1 cut(s) 328
DdeI CTNAG 4 cut(s) 63, 96, 368, 486
DpnI GATC 7 cut(s) 29, 83, 152, 318, 348, 414, 659
DpnII GATC 7 cut(s) 27, 81, 150, 316, 346, 412, 657
DraIII CACNNNGTG 1 cut(s) 325
DrdI GACNNNNNNGTC 2 cut(s) 14, 506
DseDI GACNNNNNNGTC 2 cut(s) 14, 506
Eam1104I CTCTTC 1 cut(s) 677
EarI CTCTTC 1 cut(s) 677
EciI GGCGGA 2 cut(s) 41, 152
Eco130I CCWWGG 1 cut(s) 291
Eco47I GGWCC 1 cut(s) 571
Eco57I CTGAAG 1 cut(s) 230
Eco88I CYCGRG 1 cut(s) 582
EcoRI GAATTC 1 cut(s) 104
EcoRII CCWGG 1 cut(s) 573
EcoT14I CCWWGG 1 cut(s) 291
ErhI CCWWGG 1 cut(s) 291
FaeI CATG 4 cut(s) 217, 277, 326, 548
FaiI YATR 9 cut(s) 165, 215, 275, 324, 459, 494, 537, 546, 691
FatI CATG 4 cut(s) 213, 273, 322, 544
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 5 cut(s) 125, 218, 285, 684, 687
FokI GGATG 4 cut(s) 35, 274, 599, 641
Fsp4HI GCNGC 5 cut(s) 125, 218, 285, 684, 687
GluI GCNGC 5 cut(s) 125, 218, 285, 684, 687
GsuI CTGGAG 1 cut(s) 582
HaeIII GGCC 2 cut(s) 74, 251
HapII CCGG 1 cut(s) 403
HgaI GACGC 1 cut(s) 17
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 4 cut(s) 217, 277, 326, 548
HincII GTYRAC 2 cut(s) 187, 268
HindII GTYRAC 2 cut(s) 187, 268
HindIII AAGCTT 1 cut(s) 335
HinfI GANTC 2 cut(s) 428, 440
HpaI GTTAAC 1 cut(s) 268
HpaII CCGG 1 cut(s) 403
HphI GGTGA 3 cut(s) 52, 311, 474
Hpy166II GTNNAC 4 cut(s) 187, 268, 328, 499
Hpy188I TCNGA 4 cut(s) 194, 303, 342, 360
Hpy188III TCNNGA 4 cut(s) 437, 561, 584, 653
Hpy8I GTNNAC 4 cut(s) 187, 268, 328, 499
Hpy99I CGWCG 3 cut(s) 11, 134, 191
HpyAV CCTTC 3 cut(s) 64, 166, 271
HpyCH4III ACNGT 1 cut(s) 508
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 4 cut(s) 124, 217, 620, 689
HpyF10VI GCNNNNNNNGC 3 cut(s) 31, 379, 467
HpyF3I CTNAG 4 cut(s) 63, 96, 368, 486
HpySE526I ACGT 1 cut(s) 189
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 4 cut(s) 217, 277, 326, 548
KspAI GTTAAC 1 cut(s) 268
Kzo9I GATC 7 cut(s) 27, 81, 150, 316, 346, 412, 657
LguI GCTCTTC 1 cut(s) 677
LmnI GCTCC 2 cut(s) 295, 529
Lsp1109I GCAGC 5 cut(s) 136, 229, 296, 670, 673
LweI GCATC 2 cut(s) 296, 607
MaeII ACGT 1 cut(s) 189
MalI GATC 7 cut(s) 29, 83, 152, 318, 348, 414, 659
MboI GATC 7 cut(s) 27, 81, 150, 316, 346, 412, 657
MflI RGATCY 4 cut(s) 27, 81, 346, 657
MluCI AATT 2 cut(s) 104, 361
MlyI GAGTC 1 cut(s) 437
MnlI CCTC 6 cut(s) 36, 58, 91, 127, 274, 557
MseI TTAA 2 cut(s) 237, 267
MspI CCGG 1 cut(s) 403
MspR9I CCNGG 1 cut(s) 575
MvaI CCWGG 1 cut(s) 575
MwoI GCNNNNNNNGC 3 cut(s) 31, 379, 467
NdeII GATC 7 cut(s) 27, 81, 150, 316, 346, 412, 657
NlaIII CATG 4 cut(s) 217, 277, 326, 548
NmeAIII GCCGAG 1 cut(s) 338
PaqCI CACCTGC 1 cut(s) 529
PciSI GCTCTTC 1 cut(s) 677
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PfeI GAWTC 1 cut(s) 440
PflMI CCANNNNNTGG 1 cut(s) 37
PkrI GCNGC 5 cut(s) 126, 219, 286, 685, 688
PleI GAGTC 1 cut(s) 436
PpsI GAGTC 1 cut(s) 436
Psp6I CCWGG 1 cut(s) 573
PspGI CCWGG 1 cut(s) 573
PspPI GGNCC 2 cut(s) 250, 571
PsuI RGATCY 4 cut(s) 27, 81, 346, 657
RsaI GTAC 1 cut(s) 329
RsaNI GTAC 1 cut(s) 328
SalI GTCGAC 1 cut(s) 185
SapI GCTCTTC 1 cut(s) 677
SaqAI TTAA 2 cut(s) 237, 267
SatI GCNGC 5 cut(s) 125, 218, 285, 684, 687
Sau3AI GATC 7 cut(s) 27, 81, 150, 316, 346, 412, 657
Sau96I GGNCC 2 cut(s) 250, 571
SchI GAGTC 1 cut(s) 437
ScrFI CCNGG 1 cut(s) 575
SfaNI GCATC 2 cut(s) 296, 607
SinI GGWCC 1 cut(s) 571
Sse9I AATT 2 cut(s) 104, 361
SsiI CCGC 4 cut(s) 52, 137, 461, 661
StyD4I CCNGG 1 cut(s) 573
StyI CCWWGG 1 cut(s) 291
TaaI ACNGT 1 cut(s) 508
TaiI ACGT 1 cut(s) 192
TaqI TCGA 2 cut(s) 132, 186
TaqII GACCGA 1 cut(s) 665
TasI AATT 2 cut(s) 104, 361
TatI WGTACW 1 cut(s) 327
TfiI GAWTC 1 cut(s) 440
Tru1I TTAA 2 cut(s) 237, 267
Tru9I TTAA 2 cut(s) 237, 267
TseI GCWGC 5 cut(s) 124, 217, 284, 683, 686
TspDTI ATGAA 4 cut(s) 17, 37, 290, 467
TspGWI ACGGA 2 cut(s) 76, 629
Van91I CCANNNNNTGG 1 cut(s) 37
VpaK11BI GGWCC 1 cut(s) 571
XapI RAATTY 2 cut(s) 104, 361
XmiI GTMKAC 1 cut(s) 186
ZraI GACGTC 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.