pycom06g10000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0006624
Physical Location & Seq
Forward (+)
15328989 .. 15332062
3074 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1017 bp
ATGCCCTTCACACAATGTAAAGAAGTGGGTTTGTCTCAGATCATGGAGAAGGATTTTGTTCTGTTGGTTGGGATTTTATTGTTGCAGATTGTAGTTCATTGCTCTGGTGCTGATTCCAAGGTAGAATCCAGTGTGAAAACTGATTTGGGTCGGAAAAGTTCGTTAGCATCGACTAATTCAAATGGTTCCAATGATAACACTGTTGGGTCTAATTTGGTTTTGAATGCTACCGATGTTCAAAAAGTGAAGAAGGACGGAGACCGGGTGGTTGGATCAAAGGAGGGTGATGGGGATAAGAAGAGCAATCCAAGTAAGGAAGTGGGTGTGAAAGAATCTGATAATGTGGCAAAGAGTGGGGGAGGATCGAGTGAGGGATCCAAAATAGAGGGCGAGGACAAGAAACAGAAGTTGAGTGATGGGTTGGAATCAAAGGAAGTACCTAAGGAGGTGGGTAATGGAGTAAATGCAGTCTCTGTGAACCCCATGAGGAAGGAGGGGACTCGAAGTGAGGAATGTGATCTATCTAATATGTGCACTGATAAGGGCAACCAGCTGGTTGCGTGTTTGAGAGTTCCCGGAAATGATTCTCCCCATCTTTCACTTTTAATTAAGAACAAGGCCAAAGGCCCTCTCTTGGTTACCATTGTAGCTCCTGGTTTTGTGAAACTGGACAAAACAAAAATTCAACTTGAAGAGAAAGAAAATGGAAAGGTTTTGGTTTCAATTGGAAAGGGAGGAGCTGGTGGTTCAATTGTGCTAAAAGCAGGAAATGGTCAATGCAACCTTGATTTGAAGGACCTGGTCACACACAGTTCCAGGAAGGAGCCTGAGAATTCTTCAAACTTTGCCTACACAAACTTCCTAAAACAAAAACCTACGATTGCAGTTGTGTTCTTTGCTTCAGTAGTGATATTGGCATCGGCTTGGATGTGCATCAGCTTTAGGAATAGGCGGTTCTCTGGCAATGGGTTGAAATATCAGAAGCTAGATGAGGACCTGCCAATTTCCAACGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

339

Amino Acids

36.05

Weight (kDa)

8.67

Isoelectric Point (pI)

26.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7356 PF24053 164 - 267 1.8e-31 Domain of unknown function (DUF7356)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1005
AciI CCGC 1 cut(s) 952
AclWI GGATC 4 cut(s) 280, 369, 370, 382
AcsI RAATTY 2 cut(s) 681, 832
AcuI CTGAAG 1 cut(s) 885
AfaI GTAC 1 cut(s) 438
AfiI CCNNNNNNNGG 1 cut(s) 634
AjnI CCWGG 3 cut(s) 652, 798, 815
AjuI GAANNNNNNNTTGG 2 cut(s) 128, 160
AluBI AGCT 5 cut(s) 553, 650, 740, 939, 985
AluI AGCT 5 cut(s) 553, 650, 740, 939, 985
Alw21I GWGCWC 1 cut(s) 536
Alw26I GTCTC 3 cut(s) 39, 252, 475
Alw44I GTGCAC 1 cut(s) 532
AlwI GGATC 4 cut(s) 280, 369, 370, 382
AlwNI CAGNNNCTG 1 cut(s) 473
AoxI GGCC 2 cut(s) 618, 625
ApaLI GTGCAC 1 cut(s) 532
ApoI RAATTY 2 cut(s) 681, 832
Asp700I GAANNNNTTC 1 cut(s) 583
AspS9I GGNCC 3 cut(s) 626, 796, 994
AsuC2I CCSGG 2 cut(s) 263, 576
AsuHPI GGTGA 1 cut(s) 296
AvaII GGWCC 2 cut(s) 796, 994
AxyI CCTNAGG 1 cut(s) 441
BaeGI GKGCMC 1 cut(s) 536
BamHI GGATCC 1 cut(s) 374
Bbv12I GWGCWC 1 cut(s) 536
BccI CCATC 3 cut(s) 281, 410, 600
BciT130I CCWGG 3 cut(s) 654, 800, 817
BcnI CCSGG 2 cut(s) 263, 576
BcoDI GTCTC 3 cut(s) 39, 252, 475
BfaI CTAG 1 cut(s) 986
BfuAI ACCTGC 1 cut(s) 1005
Bme1390I CCNGG 5 cut(s) 263, 576, 654, 800, 817
Bme18I GGWCC 2 cut(s) 796, 994
BmgT120I GGNCC 3 cut(s) 626, 796, 994
BmiI GGNNCC 3 cut(s) 187, 376, 825
BmrFI CCNGG 5 cut(s) 263, 576, 654, 800, 817
BmsI GCATC 3 cut(s) 176, 926, 942
BpuMI CCSGG 2 cut(s) 263, 576
BsaBI GATNNNNATC 1 cut(s) 932
BsaI GGTCTC 1 cut(s) 252
BsaJI CCNNGG 1 cut(s) 117
BsaXI ACNNNNNCTCC 2 cut(s) 249, 279
Bsc4I CCNNNNNNNGG 1 cut(s) 634
Bse1I ACTGG 2 cut(s) 129, 672
Bse21I CCTNAGG 1 cut(s) 441
Bse3DI GCAATG 2 cut(s) 97, 970
Bse8I GATNNNNATC 1 cut(s) 932
BseBI CCWGG 3 cut(s) 654, 800, 817
BseDI CCNNGG 1 cut(s) 117
BseGI GGATG 1 cut(s) 933
BseJI GATNNNNATC 1 cut(s) 932
BseLI CCNNNNNNNGG 1 cut(s) 634
BseMI GCAATG 2 cut(s) 97, 970
BseMII CTCAG 2 cut(s) 50, 819
BseNI ACTGG 2 cut(s) 129, 672
BseRI GAGGAG 1 cut(s) 750
BseSI GKGCMC 1 cut(s) 536
BshFI GGCC 2 cut(s) 620, 627
BsiHKAI GWGCWC 1 cut(s) 536
BsiSI CCGG 2 cut(s) 262, 576
BslFI GGGAC 1 cut(s) 511
BslI CCNNNNNNNGG 1 cut(s) 634
BsmAI GTCTC 3 cut(s) 39, 252, 475
BsmFI GGGAC 1 cut(s) 511
BsmI GAATGC 1 cut(s) 229
BsnI GGCC 2 cut(s) 620, 627
Bso31I GGTCTC 1 cut(s) 252
Bsp1286I GDGCHC 1 cut(s) 536
Bsp143I GATC 5 cut(s) 39, 272, 362, 374, 517
BspACI CCGC 1 cut(s) 952
BspANI GGCC 2 cut(s) 620, 627
BspCNI CTCAG 2 cut(s) 49, 820
BspLI GGNNCC 3 cut(s) 187, 376, 825
BspMI ACCTGC 1 cut(s) 1005
BspPI GGATC 4 cut(s) 280, 369, 370, 382
BspQI GCTCTTC 1 cut(s) 293
BspTNI GGTCTC 1 cut(s) 252
BsrDI GCAATG 2 cut(s) 97, 970
BsrI ACTGG 2 cut(s) 129, 672
BssECI CCNNGG 1 cut(s) 117
BssMI GATC 5 cut(s) 39, 272, 362, 374, 517
BssT1I CCWWGG 1 cut(s) 117
Bst2UI CCWGG 3 cut(s) 654, 800, 817
Bst4CI ACNGT 2 cut(s) 202, 812
Bst6I CTCTTC 2 cut(s) 293, 687
BstDEI CTNAG 3 cut(s) 36, 441, 828
BstEII GGTNACC 1 cut(s) 637
BstF5I GGATG 1 cut(s) 933
BstKTI GATC 5 cut(s) 42, 275, 365, 377, 520
BstMAI GTCTC 3 cut(s) 39, 252, 475
BstMBI GATC 5 cut(s) 39, 272, 362, 374, 517
BstNI CCWGG 3 cut(s) 654, 800, 817
BstPI GGTNACC 1 cut(s) 637
BstSCI CCNGG 5 cut(s) 261, 574, 652, 798, 815
BstSLI GKGCMC 1 cut(s) 536
BstX2I RGATCY 1 cut(s) 374
BstYI RGATCY 1 cut(s) 374
Bsu36I CCTNAGG 1 cut(s) 441
BsuRI GGCC 2 cut(s) 620, 627
BtsCI GGATG 1 cut(s) 933
BtsIMutI CAGTG 3 cut(s) 136, 198, 534
BveI ACCTGC 1 cut(s) 1005
CaiI CAGNNNCTG 1 cut(s) 473
Cfr13I GGNCC 3 cut(s) 626, 796, 994
CsiI ACCWGGT 1 cut(s) 798
Csp6I GTAC 1 cut(s) 437
CviAII CATG 2 cut(s) 43, 484
CviJI RGCY 9 cut(s) 553, 620, 627, 650, 740, 826, 923, 939, 985
CviKI_1 RGCY 9 cut(s) 553, 620, 627, 650, 740, 826, 923, 939, 985
CviQI GTAC 1 cut(s) 437
DdeI CTNAG 3 cut(s) 36, 441, 828
DpnI GATC 5 cut(s) 41, 274, 364, 376, 519
DpnII GATC 5 cut(s) 39, 272, 362, 374, 517
Eam1104I CTCTTC 2 cut(s) 293, 687
EarI CTCTTC 2 cut(s) 293, 687
Eco130I CCWWGG 1 cut(s) 117
Eco31I GGTCTC 1 cut(s) 252
Eco47I GGWCC 2 cut(s) 796, 994
Eco57I CTGAAG 1 cut(s) 885
Eco81I CCTNAGG 1 cut(s) 441
Eco91I GGTNACC 1 cut(s) 637
EcoO109I RGGNCCY 3 cut(s) 626, 796, 994
EcoO65I GGTNACC 1 cut(s) 637
EcoRI GAATTC 1 cut(s) 832
EcoRII CCWGG 3 cut(s) 652, 798, 815
EcoT14I CCWWGG 1 cut(s) 117
ErhI CCWWGG 1 cut(s) 117
FaeI CATG 2 cut(s) 46, 487
FaiI YATR 3 cut(s) 44, 485, 530
FaqI GGGAC 1 cut(s) 511
FatI CATG 2 cut(s) 42, 483
FokI GGATG 1 cut(s) 940
FspBI CTAG 1 cut(s) 986
HaeIII GGCC 2 cut(s) 620, 627
HapII CCGG 2 cut(s) 262, 576
Hin1II CATG 2 cut(s) 46, 487
HinfI GANTC 6 cut(s) 113, 125, 332, 425, 499, 584
HpaII CCGG 2 cut(s) 262, 576
HphI GGTGA 1 cut(s) 296
Hpy166II GTNNAC 2 cut(s) 478, 534
Hpy188I TCNGA 4 cut(s) 39, 153, 337, 981
Hpy8I GTNNAC 2 cut(s) 478, 534
HpyAV CCTTC 6 cut(s) 16, 43, 244, 484, 787, 814
HpyCH4III ACNGT 2 cut(s) 202, 812
HpyCH4V TGCA 6 cut(s) 85, 467, 534, 780, 884, 933
HpyF3I CTNAG 3 cut(s) 36, 441, 828
Hsp92II CATG 2 cut(s) 46, 487
Kzo9I GATC 5 cut(s) 39, 272, 362, 374, 517
LguI GCTCTTC 1 cut(s) 293
LmnI GCTCC 3 cut(s) 655, 737, 823
LweI GCATC 3 cut(s) 176, 926, 942
MabI ACCWGGT 1 cut(s) 798
MaeI CTAG 1 cut(s) 986
MaeIII GTNAC 2 cut(s) 637, 802
MalI GATC 5 cut(s) 41, 274, 364, 376, 519
MboI GATC 5 cut(s) 39, 272, 362, 374, 517
MboII GAAGA 4 cut(s) 259, 310, 704, 828
MfeI CAATTG 2 cut(s) 723, 750
MflI RGATCY 1 cut(s) 374
MhlI GDGCHC 1 cut(s) 536
MluCI AATT 8 cut(s) 175, 211, 606, 681, 723, 750, 832, 1002
MlyI GAGTC 1 cut(s) 493
MmeI TCCRAC 3 cut(s) 131, 250, 402
MroXI GAANNNNTTC 1 cut(s) 583
MseI TTAA 2 cut(s) 605, 609
MspA1I CMGCKG 1 cut(s) 553
MspI CCGG 2 cut(s) 262, 576
MspR9I CCNGG 5 cut(s) 263, 576, 654, 800, 817
MunI CAATTG 2 cut(s) 723, 750
Mva1269I GAATGC 1 cut(s) 229
MvaI CCWGG 3 cut(s) 654, 800, 817
NciI CCSGG 2 cut(s) 263, 576
NdeII GATC 5 cut(s) 39, 272, 362, 374, 517
NlaIII CATG 2 cut(s) 46, 487
NlaIV GGNNCC 3 cut(s) 187, 376, 825
NmuCI GTSAC 1 cut(s) 802
PacI TTAATTAA 1 cut(s) 609
PciSI GCTCTTC 1 cut(s) 293
PcsI WCGNNNNNNNCGW 1 cut(s) 167
PctI GAATGC 1 cut(s) 229
PdmI GAANNNNTTC 1 cut(s) 583
PfeI GAWTC 5 cut(s) 113, 125, 332, 425, 584
PflFI GACNNNGTC 1 cut(s) 800
PfoI TCCNGGA 2 cut(s) 574, 815
PleI GAGTC 1 cut(s) 493
PpsI GAGTC 1 cut(s) 493
PpuMI RGGWCCY 2 cut(s) 796, 994
Psp5II RGGWCCY 2 cut(s) 796, 994
Psp6I CCWGG 3 cut(s) 652, 798, 815
PspEI GGTNACC 1 cut(s) 637
PspGI CCWGG 3 cut(s) 652, 798, 815
PspN4I GGNNCC 3 cut(s) 187, 376, 825
PspPI GGNCC 3 cut(s) 626, 796, 994
PspPPI RGGWCCY 2 cut(s) 796, 994
PstNI CAGNNNCTG 1 cut(s) 473
PsuI RGATCY 1 cut(s) 374
PsyI GACNNNGTC 1 cut(s) 800
PvuII CAGCTG 1 cut(s) 553
RsaI GTAC 1 cut(s) 438
RsaNI GTAC 1 cut(s) 437
SapI GCTCTTC 1 cut(s) 293
SaqAI TTAA 2 cut(s) 605, 609
Sau3AI GATC 5 cut(s) 39, 272, 362, 374, 517
Sau96I GGNCC 3 cut(s) 626, 796, 994
SchI GAGTC 1 cut(s) 493
ScrFI CCNGG 5 cut(s) 263, 576, 654, 800, 817
SduI GDGCHC 1 cut(s) 536
SexAI ACCWGGT 1 cut(s) 798
SfaNI GCATC 3 cut(s) 176, 926, 942
SinI GGWCC 2 cut(s) 796, 994
Sse9I AATT 8 cut(s) 175, 211, 606, 681, 723, 750, 832, 1002
SsiI CCGC 1 cut(s) 952
SspMI CTAG 1 cut(s) 986
StyD4I CCNGG 5 cut(s) 261, 574, 652, 798, 815
StyI CCWWGG 1 cut(s) 117
TaaI ACNGT 2 cut(s) 202, 812
TaqI TCGA 3 cut(s) 170, 365, 502
TasI AATT 8 cut(s) 175, 211, 606, 681, 723, 750, 832, 1002
TfiI GAWTC 5 cut(s) 113, 125, 332, 425, 584
Tru1I TTAA 2 cut(s) 605, 609
Tru9I TTAA 2 cut(s) 605, 609
TscAI CASTG 3 cut(s) 136, 205, 541
TseFI GTSAC 1 cut(s) 802
Tsp45I GTSAC 1 cut(s) 802
TspDTI ATGAA 1 cut(s) 86
TspGWI ACGGA 1 cut(s) 270
TspRI CASTG 3 cut(s) 136, 205, 541
Tth111I GACNNNGTC 1 cut(s) 800
VneI GTGCAC 1 cut(s) 532
VpaK11BI GGWCC 2 cut(s) 796, 994
XapI RAATTY 2 cut(s) 681, 832
XmnI GAANNNNTTC 1 cut(s) 583
XspI CTAG 1 cut(s) 986
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.